Native American Y Haplogroup C-P39 Sprouts Branches!

I am extremely pleased to provide an update on the Haplogroup C-P39 Native American Y DNA project. Marie Rundquist and I as co-administrators have exciting discoveries to share.

As it so happens, this announcement comes almost exactly on the 4th anniversary of the founding of this project at Family Tree DNA. We couldn’t celebrate in a better way!

Native American Y DNA Haplogroups

Haplogroup C is one of two core Native American male haplogroups. Of the two, haplogroup Q is much more prevalent, while haplogroup C is rare. Only some branches of both haplogroup Q and haplogroup C are Native American, with other branches of both haplogroups being Asian and European.

C-P39 is the Native American branch of haplogroup C, and because of its rarity, until now, very little was known. There were no known branches.

In February 2016, Marie Rundquist created a focused project testing plan to upgrade at least one man from each family line to the full 111 markers along with a Big Y test in order to determine if further differentiation could be achieved in the C-P39 haplogroup lineage.

Haplogroup C-P39 Sprouts Branches

In November 2016, Marie presented preliminary research findings at the International Genetic Genealogy Conference in Houston, Texas, with a final evaluation being completed and submitted to Family Tree DNA for review in March 2017. As a result, Marie provides the following press release:

April 29, 2017: Based on a recent “Big Y” DNA novel variant submission from the C-P39 Y DNA project, the Y Tree has been updated by Family Tree DNA scientists. With this latest update, in addition to the C-P39 SNP that distinguishes this haplogroup, there are now new, long-awaited, downstream SNPs and subclades, as reflected in the Y Tree that offer new avenues for research by members of this rare, Native American haplogroup. A summary of new C-P39 Y DNA project subclades follows:

  • North American Appalachian Region: C-P39+ C-BY1360+
  • North American Canada – Multiple Surnames: C-P39+ C-Z30765+
  • North American Canada – Multiple Surnames: C-P39+ C-Z30750+
  • North American Canada: Acadia (Nova Scotia): C-P39+ C-Z30750+
  • North American Canada: Acadia (Nova Scotia): C-P39+ C-Z30754+
  • North American Southwest Region: CP39+ C-Z30747+

The following SNP (BY18405+) was found to have been shared only by two C-P39 project members in the entire Big Y system, as reported here:

  • North American Canada Newfoundland: C-P39+ C-BY18405+
  • North American Canada: Gaspe, QC: C-P39+ C-BY18405+

The ancestors of two families represented in the study, one in the Pacific Northwest and another in the North American Southwest did not experience any mutations in the New World and Big Y results are within the current genetic boundaries of the C-P39 SNP haplogroup as noted.

The Family Tree DNA C-P39 Y DNA Project is managed by Roberta Estes, Administrator, Marie Rundquist, Co-Administrator, and Dr. David Pike, Project Advisor. The “Big Y” DNA test is a product of Family Tree DNA.

Reference: https://www.familytreedna.com/public/ydna_C-P39

The New Tree

The new C-P39 tree at Family Tree DNA is shown, below, including all the new SNPs below P39, a grand total of eight new branches on the C-P39 tree.

It’s just so beautiful to see this in black and white – well, green, black and white. It’s really an amazing accomplishment for citizen scientists to be contributing at this level to the field of genetics.

Beneath C-P39, several sub-branches develop.

  • BY1360 which is represented by a gentleman from Appalachia.
  • BY736 which is represented by two downstream SNPs that include the surnames of both King and Brooms from Canada.
  • Z30747 which is represented by a Garcia from the southwest US, following by downstream subgroup Z30750 represented by a Canadian gentleman, and SNP Z30754 represented by the Acadian Doucette family from Nova Scotia.

This haplotree suggests that the SNP carried by the gentleman from Appalachia is the oldest, with the other sub-branches descending from their common ancient lineage. As you might guess, this isn’t exactly what we had anticipated, but therein lies the thrill of discovery and the promise of science.

The Next Step

Just like with traditional genealogy, this discovery begets more questions. Now, testing needs to be done on additional individuals to see if we can further tease apart relationships and perhaps identify patterns to suggest a migration path. This testing will come, in part, from STR marker testing along with Big Y testing for some lines not yet tested at that level.

We’re also hopeful, of course, that anyone who carries haplogroup C-P39 or any downstream branch will join the C-P39 project. Collaboration is key to discovery.

Contributing

If you would like to donate to the C-P39 project general fund to play a critical role in the next steps of discovery, we would be eternally grateful. At this point, we need to fund at least 4 additional Big Y tests, plus several 111 marker upgrades, totaling about $3000. You can contribute to the project general fund at this link:

https://www.familytreedna.com/group-general-fund-contribution.aspx?g=Y-DNAC-P39

Thank you in advance – every little bit helps!

Kudos

I want to personally congratulate Marie for her hard work and dedication over the past year to bring this monumental discovery and tree update to fruition. It’s truly an incredible accomplishment representing countless hours of behind the scenes work.

Marie and I would both like to thank all of our participants, individuals who contributed funds to the testing, Dr. David Pike as a project advisor and, of course, Family Tree DNA, without whom none of this would be possible.

DNA Testing for Native Heritage

If you are male and have not yet Y DNA tested, but believe that you have a Native ancestor on your direct paternal (surname) line, please order at least the 37 marker test at Family Tree DNA. Your results and who you match will tell that story!

People with Native heritage on any ancestral line are encouraged to join the American Indian Project at Family Tree DNA. If you have tested elsewhere, you can download your results to Family Tree DNA for free.

For additional information about DNA testing for Native American heritage, please read Proving Native American Ancestry Using DNA.

Concepts – Percentage of Ancestors’ DNA

A very common question is, “How much DNA of an ancestor do I carry and how does that affect my ethnicity results?”

This question is particularly relevant for people who are seeking evidence of a particular ethnicity of an ancestor several generations back in time. I see this issue raise its head consistently when people take an ethnicity test and expect that their “full blood” Native American great-great-grandmother will show up in their results.

Let’s take a look at how DNA inheritance works – and why they might – or might not find the Native DNA they seek, assuming that great-great-grandma actually was Native.

Inheritance

Every child inherits exactly 50% of their autosomal DNA from each parent (except for the X chromosome in males.) However, and this is a really important however, the child does NOT inherit exactly half of the DNA of each ancestor who lived before the parents. How can this be, you ask?

Let’s step through this logically.

The number of ancestors you have doubles in each generation, going back in time.

This chart provides a summary of how many ancestors you have in each generation, an approximate year they were born using a 25 year generation and a 30 year generation, respectively, and how much of their DNA, on average, you could expect to carry, today. You’ll notice that by the time you’re in the 7th generation, you can be expected, on average, to carry 0.78% meaning less than 1% of that GGGGG-grandparent’s DNA.

Looking at the chart, you can see that you reach the 1% level at about the 6th generation with an ancestor probably born in the late 1700s or early 1800s.

It’s also worth noting here that generations can be counted differently. In some instances, you are counted as generation one, so your GGGGG-grandparent would be generation 8.

In general, DNA showing ethnicity below about 5% is viewed as somewhat questionable and below 2% is often considered to be “noise.” Clearly, that isn’t always the case, especially if you are dealing with continental level breakdowns, as opposed to within Europe, for example. Intra-continental (regional) ethnicity breakdowns are particularly difficult and unreliable, but continental level differences are easier to discern and are considered to be more reliable, comparatively.

If you want to learn more about how ethnicity calculations are derived and what they mean, please read the article Ethnicity Testing – A Conundrum.

On Average May Not Mean You

On average, each child receives half of the DNA of each ancestor from their parent.

The words “on average” are crucial to this discussion, because the average assumes that in fact each generation between your GGGGG-grandmother and you inherited exactly half of the DNA in each generation from their parent that was contributed by that GGGGG-grandmother.

Unfortunately, while averages are all that we have to work with, that’s not always how ancestral DNA is passed in each generation.

Let’s say that your GGGGG-grandmother was indeed full Native, meaning no admixture at all.

You can click to enlarge images.

Using the chart above, you can see that your GGGGG-grandmother was full native on all 20 “pieces” or segments of DNA used for this illustration. Those segments are colored red. The other 10 segments, with no color, were contributed by the father.

Let’s say she married a person who was not Native, and in every generation since, there were no additional Native ancestors.

Her child, generation 6, inherited exactly 50% of her DNA, shown in red – meaning 10 segments..

Generation 5, her grandchild, inherited exactly half of her DNA that was carried by the parent, shown in red – meaning 5 segments..

However, in the next generation, generation 4, that child inherited more than half of the Native DNA from their parent. They inherited half of their parent’s DNA, but the half that was randomly received included 3 Native segments out of a possible 5 Native segments that the parent carried.

In generation 3, that child inherited 2 of the possible 3 segments that their parent carried.

In generation 2, that person inherited all of the Native DNA that their parent carried.

In generation 1, your parent inherited half of the DNA that their parent carried, meaning one of 2 segments of Native DNA carried by your grandparent.

And you will either receive all of that one segment, part of that one segment, or none of that one segment.

In the case of our example, you did not inherit that segment, which is why you show no Native admixture, even though your GGGGG-grandmother was indeed fully Native..

Of course, even if you had inherited that Native segment, and that segment isn’t something the population reference models recognize as “Native,” you still won’t show as carrying any Native at all. It could also be that if you had inherited the red segment, it would have been too small and been interpreted as noise.

The “Received” column at the right shows how much of the ancestral DNA the current generation received from their parent.

The “% of Original” column shows how the percentage of GGGGG-grandmother’s DNA is reduced in each generation.

The “Expected” column shows how much DNA, “on average” we would expect to see in each generation, as compared to the “% of Original” which is how much they actually carry.

I intentionally made the chart, above, reflect a scenario close to what we could expect, on average. However, it’s certainly within the realm of possibility to see something like the following scenario, as well.

In the second example, above, neither you nor your parent or grandparent inherited any of the Native segments.

It’s also possible to see a third example, below, where 4 generations in a row, including you, inherited the full amount of Native DNA segments carried by the GG-grandparent.

Testing Other Relatives

Every child of every couple inherits different DNA from their parents. The 50% of their parents’ DNA that they inherit is not all the same. The three example charts above could easily represent three children of the GG-Grandparent and their descendants.

The pedigree chart below shows the three different examples, above.  The great-great-grandparent in the 4th generation who inherited 3 Native DNA segments is shown first, then the inheritance of the Native segments through all 3 children to the current generation.

Therefore, you may not have inherited the red segment of GGGGG-grandmother’s Native DNA, but your sibling might, or vice versa. As you can see in the chart above, one of your third cousins received 3 native segments from GGGGG-grandmother. but your other third cousin received none.

You can see why people are always encouraged to test their parents and grandparents as well as siblings. You never know where your ancestor’s DNA will turn up, and each person will carry a different amount, and different segments of DNA from your common ancestors.

In other words, your great-aunt and great-uncle’s DNA is every bit as important to you as your own grandparent’s DNA – so test everyone in older generations while you can, and their children if they are no longer available.

Back to Great-Great-Grandma

Going back to great-great-grandma and her Native heritage. You may not show Native ethnicity when you expected to see Native, but you may have other resources and recourses. Don’t give up!

Reason Resources and Comments
She really wasn’t Native. Genealogical research will help and mitochondrial DNA testing of an appropriate descendant will point the way to her true ethnic heritage, at least on her mother’s side.
She was Native, but the ethnicity test doesn’t show that I am. Test relatives and find someone descended from her through all females to take a mitochondrial test. The mitochondrial test will answer the question for her matrilineal line unquestionably.
She was partly, but not fully Native. This would mean that she had less Native DNA than you thought, which would mean the percentage coming to you is lower on average than anticipated. Mitochondrial DNA testing someone descended from her through all females to the current generation, which can be male, would reveal whether her mother was Native from her mother’s line.
She was Native, but several generations back in time. You or your siblings may show small percentages of Native or other locations considered to be a component of Native admixture in the absence of any other logical explanation for their presence, such as Siberian or Eastern Asian.

Using Y and Mitochondrial DNA Testing to Supplement Ethnicity Testing

When in doubt about ethnicity results, find an appropriately descended person to take a Y DNA test (males only, for direct paternal lineage) or a mitochondrial DNA test, for direct matrilineal results. These tests will yield haplogroup information and haplogroups are associated with specific world regions and ethnicities, providing a more definitive answer regarding the heritage of that specific line.

Y DNA reflects the direct male line, shown in blue above, and mitochondrial DNA reflects the direct matrilineal line, shown in red. Only males carry Y DNA, but both genders carry mitochondrial DNA.

For a short article about the different kinds of DNA and how they can help genealogists, please read 4 Kinds of DNA for Genetic Genealogy.

Ethnicity testing is available from any of the 3 major vendors, meaning Family Tree DNA, Ancestry or 23andMe. Base haplogroups are provided with 23andMe results, but detailed testing for Y and mitochondrial DNA is only available from Family Tree DNA.

To read about the difference between the two types of testing utilized for deriving haplogroups between 23andMe and Family Tree DNA, please read Haplogroup Comparisons between Family Tree DNA and 23andMe.

For more information on haplogroups, please read What is a Haplogroup?

For a discussion about testing family members, please read Concepts – Why DNA Testing the Oldest Family Members is Critically Important.

If you’d like to read a more detailed explanation of how inheritance works, please read Concepts – How Your Autosomal DNA Identifies Your Ancestors.

Jessica Biel – A Follow-up: DNA, Native Heritage and Lies

Jessica Biel’s episode aired on Who Do You Think You Are on Sunday, April 2nd. I wanted to write a follow-up article since I couldn’t reveal Jessica’s Native results before the show aired.

The first family story about Jessica’s Biel line being German proved to be erroneous. In total, Jessica had three family stories she wanted to follow, so the second family legend Jessica set out to research was her Native American heritage.

I was very pleased to see a DNA test involved, but I was dismayed that the impression was left with the viewing audience that the ethnicity results disproved Jessica’s Native heritage. They didn’t.

Jessica’s Ethnicity Reveal

Jessica was excited about her DNA test and opened her results during the episode to view her ethnicity percentages.

Courtesy TLC

The locations shown below and the percentages, above, show no Native ethnicity.

Courtesy TLC

Jessica was understandably disappointed to discover that her DNA did not reflect any Native heritage – conflicting with her family story. I feel for you Jessica.  Been there, done that.

Courtesy TLC

Jessica had the same reaction of many of us. “Lies, lies,” she said, in frustration.

Well Jessica, maybe not.

Let’s talk about Jessica’s DNA results.

Native or Lies?

I’ve written about the challenges with ethnicity testing repeatedly. At the end of this article, I’ll provide a reading resource list.

Right now, I want to talk about the misperception that because Jessica’s DNA ethnicity results showed no Native, that her family story about Native heritage is false. Even worse, Jessica perceived those stories to be lies. Ouch, that’s painful.

In my world view, a lie is an intentional misrepresentation of the truth. Let’s say that Jessica really didn’t have Native heritage. That doesn’t mean someone intentionally lied. People might have been confused. Maybe they made assumptions. Sometimes facts are misremembered or misquoted. I always give my ancestors the benefit of the doubt unless there is direct evidence of an intentional lie. And if then, I would like to try to understand what prompted that behavior. For example, discrimination encouraged many people of mixed ethnicity to “pass” for white as soon as possible.

That’s certainly a forgivable “lie.”

Ok, Back to DNA

Autosomal DNA testing can only reliably pick up to about the 1% level of minority DNA admixture successfully – minority meaning a small amount relative to your overall ancestry.

Everyone inherits DNA from ancestors differently, in different amounts, in each generation. Remember, you receive half of your DNA from each parent, but which half of their DNA you receive is random. That holds true for every generation between the ancestor in question and Jessica today.  Ultimately, more or less than 50% of any ancestor’s DNA can be passed in any generation.

However, if Jessica inherited the average amount of DNA from each generation, being 50% of the DNA from the ancestor that the parent had, the following chart would represent the amount of DNA Jessica carried from each ancestor in each generation.

This chart shows the amount of DNA of each ancestor, by generation, that an individual testing today can expect to inherit, if they inherit exactly 50% of that ancestor’s DNA from the previous generation. That’s not exactly how it works, as we’ll see in a minute, because sometimes you inherit more or less than 50% of a particular ancestor’s DNA.

Utilizing this chart, in the 4th generation, Jessica has 16 ancestors, all great-great-grandparents. On average, she can expect to inherit 6.25% of the DNA of each of those ancestors.

In the rightmost column, I’ve shown Jessica’s relationship to her Jewish great-great-grandparents, shown in the episode, Morris and Ottilia Biel.

Jessica has two great-great-grandparents who are both Jewish, so the amount of Jewish DNA that Jessica would be expected to carry would be 6.25% times two, or 12.50%. But that’s not how much Jewish DNA Jessica received, according to Ancestry’s ethnicity estimates. Jessica received only 8% Jewish ethnicity, 36% less than average for having two Jewish great-great-grandparents.

Courtesy TLC

Now we know that Jessica carries less Jewish DNA that we would expect based on her proven genealogy.  That’s the nature of random recombination and how autosomal DNA works.

Now let’s look at the oral history of Jessica’s Native heritage.

Native Heritage

The intro didn’t tell us much about Jessica’s Native heritage, except that it was on her mother’s mother’s side. We also know that the fully Native ancestor wasn’t her mother or grandmother, because those are the two women who were discussing which potential tribe the ancestor was affiliated with.

We can also safely say that it also wasn’t Jessica’s great-grandmother, because if her great-grandmother had been a member of any tribe, her grandmother would have known that. I’d also wager that it wasn’t Jessica’s great-great-grandmother either, because most people would know if their grandmother was a tribal member, and Jessica’s grandmother didn’t know that. Barring a young death, most people know their grandmother. Utilizing this logic, we can probably safely say that Jessica’s Native ancestor was not found in the preceding 4 generations, as shown on the chart below.

On this expanded chart, I’ve included the estimated birth year of the ancestor in that particular generation, using 25 years as the average generation length.

If we use the logic that the fully Native ancestor was not between Jessica and her great-great-grandmother, that takes us back through an ancestor born in about 1882.

The next 2 generations back in time would have been born in 1857 and 1832, respectively, and both of those generations would have been reflected as Indian on the 1850 and/or 1860 census. Apparently, they weren’t or the genealogists working on the program would have picked up on that easy tip.

If Jessica’s Native ancestor was born in the 7th generation, in about 1807, and lived to the 1850 census, they would have been recorded in that census as Native at about 43 years of age. Now, it’s certainly possible that Jessica had a Native ancestor that might have been born about 1807 and didn’t live until the 1850 census, and whose half-Native children were not enumerated as Indian.

So, let’s go with that scenario for a minute.

If that was the case, the 7th generation born in 1807 contributed approximately 0.78% DNA to Jessica, IF Jessica inherited 50% in each generation. At 0.78%, that’s below the 1% level. Small amounts of trace DNA are reported as <1%, but at some point the amount is too miniscule to pick up or may have washed out entirely.

Let’s add to that scenario. Let’s say that Jessica’s ancestor in the 7th generation was already admixed with some European. Traders were well known to marry into tribes. If Jessica’s “Native” ancestor in the 7th generation was already admixed, that means Jessica today would carry even less than 0.78%.

You can easily see why this heritage, if it exists, might not show up in Jessica’s DNA results.

No Native DNA Does NOT Equal No Native Heritage

However, the fact that Jessica’s DNA ethnicity results don’t indicate Native American DNA doesn’t necessarily mean that Jessica doesn’t have a Native ancestor.

It might mean that Jessica doesn’t have a Native ancestor. But it might also mean that Jessica’s DNA can’t reliably disclose or identify Native ancestry that far back in time – both because of the genetic distance and also because Jessica may not have inherited exactly half of her ancestor’s Native DNA. Jessica’s 8% Jewish DNA is the perfect example of the variance in how DNA is actually passed versus the 50% average per generation that we have to utilize when calculating expected estimates.

Furthermore, keep in mind that all ethnicity tools are imprecise.  It’s a new field and the reference panels, especially for Native heritage, are not as robust as other groups.

Does Jessica Have Native Heritage?

I don’t know the answer to that question, but here’s what I do know.

  • You can’t conclude that because the ethnicity portion of a DNA test doesn’t show Native ancestry that there isn’t any.
  • You can probably say that any fully Native ancestor is not with in the past 6 generations, give or take a generation or so.
  • You can probably say that any Native ancestor is probably prior to 1825 or so.
  • You can look at the census records to confirm or eliminate Native ancestors in many or most lines within the past 6 or 7 generations.
  • You can utilize geographic location to potentially eliminate some ancestors from being Native, especially if you have a potential tribal affiliation. Let’s face it, Cherokees are not found in Maine, for example.
  • You can potentially utilize Y and mitochondrial DNA to reach further back in time, beyond what autosomal DNA can tell you.
  • If autosomal DNA does indicate Native heritage, you can utilize traditional genealogy research in combination with both Y and mitochondrial DNA to prove which line or lines the Native heritage came from.

Mitochondrial and Y DNA Testing

While autosomal DNA is constrained to 5 or 6 generations reasonably, Y and mitochondrial DNA is not.

Of course, Ancestry, who sponsors the Who Do You Think You Are series, doesn’t sell Y or mitochondrial DNA tests, so they certainly aren’t going to introduce that topic.

Y and mitochondrial DNA tests reach back time without the constraint of generations, because neither Y nor mitochondrial DNA are admixed with the other parent.

The Y DNA follows the direct paternal line for males, and mitochondrial DNA follows the direct matrilineal line for both males and females.

In the Concepts – Who To Test article, I discussed all three types of testing and who one can test to discover their heritage, through haplogroups, of each family line.  Every single one of your ancestors carried and had the opportunity to pass on either Y or mitochondrial DNA to their descendants.  Males pass the Y chromosome to male children, only, and females pass mitochondrial DNA to both genders of their children, but only females pass it on.

I don’t want to repeat myself about who carries which kind of DNA, but I do want to say that in Jessica’s case, based on what is known about her family, she could probably narrow the source of the potential Native ancestor significantly.

In the above example, if Jessica is the daughter – let’s say that we think the Native ancestor was the mother of the maternal great-grandmother. She is the furthest right on the chart, above. The pink coloring indicates that the pink maternal great grandmother carries the mitochondrial DNA and passed it on to the maternal grandmother who passed it to the mother who passed it to both Jessica and her siblings.

Therefore, Jessica or her mother, either one, could take a mitochondrial DNA test to see if there is deeper Native ancestry than an autosomal test can reveal.

When Y and mitochondrial DNA is tested, a haplogroup is assigned, and Native American haplogroups fall into subgroups of Y haplogroups C and Q, and subgroups of mitochondrial haplogroups A, B, C, D, X and probably M.

With a bit of genealogy work and then DNA testing the appropriate descendants of Jessica’s ancestors, she might still be able to discern whether or not she has Native heritage. All is not lost and Jessica’s Native ancestry has NOT been disproven – even though that’s certainly the impression left with viewers.

Y and Mitochondrial DNA Tests

If you’d like to order a Y or mitochondrial DNA test, I’d recommend the Full Mitochondrial Sequence test or the 37 marker Y DNA test, to begin with. You will receive a full haplogroup designation from the mitochondrial test, plus matching and other tools, and a haplogroup estimate with the Y DNA test, plus matching and other tools.

You can click here to order the mitochondrial DNA, the Y DNA or the Family Finder test which includes ethnicity estimates from Family Tree DNA. Family Tree DNA is the only DNA testing company that performs the Y and mitochondrial DNA tests.

Further Reading:

If you’d like to read more about ethnicity estimates, I’d specifically recommend “DNA Ethnicity Testing – A Conundrum.

If you’d like more information on how to figure out what your ethnicity estimates should be, I’d recommend Concepts – Calculating Ethnicity Percentages.

You can also search on the word “ethnicity” in the search box in the upper right hand corner of the main page of this blog.

If you’d like to read more about Native American heritage and DNA testing, I’d  recommend the following articles. You can also search for “Native” in the search box as well.

How Much Indian Do I Have In Me?

Proving Native American Ancestry Using DNA

Finding Your American Indian Tribe Using DNA

Native American Mitochondrial Haplogroups

New Native American Mitochondrial DNA Haplogroups

At the November 2016 Family Tree DNA International Conference on Genetic Genealogy, I was invited to give a presentation about my Native American research findings utilizing the Genographic Project data base in addition to other resources. I was very pleased to be offered the opportunity, especially given that the 2016 conference marked the one year anniversary of the Genographic Project Affiliate Researcher program.

The results of this collaborative research effort have produced an amazing number of newly identified Native American mitochondrial haplogroups. Previously, 145 Native American mitochondrial haplogroups had been identified. This research project increased that number by 79% added another 114 haplogroups, raising the total to 259 Native American haplogroups.

Guilt by Genetic Association

Bennett Greenspan, President of Family Tree DNA, gave a presentation several years ago wherein he described genetic genealogy as “guilt by genetic association.” This description of genetic genealogy is one of the best I have ever heard, especially as it pertains to the identification of ancestral populations by Y and mitochondrial DNA.

As DNA testing has become more mainstream, many people want to see if they have Native ancestry. While autosomal DNA can only measure back in time relative to ethnicity reliably about 5 or 6 generations, Y and mitochondrial DNA due to their unique inheritance paths and the fact that they do not mix with the other parent’s DNA can peer directly back in time thousands of years.

Native American Mitochondrial DNA

Native American mitochondrial DNA consists of five base haplogroups, A, B, C, D and X. Within those five major haplogroups are found many Native as well as non-Native sub-haplogroups. Over the last 15 years, researchers have been documenting haplogroups found within the Native community although progress has been slow for various reasons, including but not limited to the lack of participants with proven Native heritage on the relevant matrilineal genealogical line.

In the paper, “Large scale mitochondrial sequencing in Mexican Americans suggests a reappraisal of Native American origins,” published in 2011, Kumar et al state the following:

For mtDNA variation, some studies have measured Native American, European and African contributions to Mexican and Mexican American populations, revealing 85 to 90% of mtDNA lineages are of Native American origin, with the remainder having European (5-7%) or African ancestry (3-5%). Thus the observed frequency of Native American mtDNA in Mexican/Mexican Americans is higher than was expected on the basis of autosomal estimates of Native American admixture for these populations i.e. ~ 30-46%. The difference is indicative of directional mating involving preferentially immigrant men and Native American women.

The actual Native mtDNA rate in their study of 384 completely sequenced Mexican genomes was 83.3% with 3.1% being African and 13.6% European.

This means that Mexican Americans and those south of the US in Mesoamerica provide a virtually untapped resource for Native American mitochondrial DNA.

The Genographic Project Affiliate Researcher Program

At the Family Tree DNA International Conference in November 2015, Dr. Miguel Vilar announced that the Genographic Project data base would be made available for qualified affiliate researchers outside of academia. There is, of course, an application process and aspiring affiliate researchers are required to submit a research project plan for consideration.

I don’t know if I was the first applicant, but if not, I was certainly one of the first because I wasted absolutely no time in submitting my application. In fact, my proposal likely arrived in Washington DC before Dr. Vilar did!

One of my original personal goals for genetic genealogy was to identify my Native American ancestors. It didn’t take long before I realized that one of the aspects of genetic genealogy where we desperately needed additional research was relative to Native people, specifically within Native language groups or tribes and from individuals who unquestionably know their ancestry and can document that their direct Y or mtDNA ancestors were Native.

Additionally, we needed DNA from pre-European-contact burials to ascertain whether haplogroups found in Europe and Africa were introduced into the Native population post-contact or existed within the Native population as a result of a previously unknown/undocumented contact. Some of both of these types of research has occurred, but not enough.

Slowly, over the years, additional sub-haplogroups have been added for both the Y and mitochondrial Native DNA. In 2007, Tamm et al published the first comprehensive paper providing an overview of the migration pathways and haplogroups in their landmark paper, “Beringian Standstill and the Spread of Native American Founders.” Other research papers have added to that baseline over the years.

beringia map

“Beringian Standstill and the Spread of Native American Founders” by Tamm et al

In essence, whether you are an advocate of one migration or multiple migration waves, the dates of 10,000 to 25,000 years ago are a safe range for migration from Asia, across the then-present land-mass, Beringia, into the Americas. Recently another alternative suggesting that the migration may have occurred by water, in multiple waves, following coastlines, has been proposed as well – but following the same basic pathway. It makes little difference whether the transportation method was foot or kayak, or both, or one or more migration events. Our interest lies in identifying which haplogroups arrived with the Asians who became the indigenous people of the Americas.

Haplogroups

To date, proven base Native haplogroups are:

Y DNA:

  • Q
  • C

Mitochondrial DNA

  • A
  • B
  • C
  • D
  • X

Given that the Native, First Nations or aboriginal people, by whatever name you call them, descended from Asia, across the Beringian land bridge sometime between roughly 10,000 and 25,000 years ago, depending on which academic model you choose to embrace, none of the base haplogroups shown above are entirely Native. Only portions, meaning specific subgroups, are known to be Native, while other subgroups are Asian and often European as well. The descendants of the base haplogroups, all born in Asia, expanded North, South, East and West across the globe. Therefore, today, it’s imperative to test mitochondrial DNA to the full sequence level and undergo SNP testing for Y DNA to determine subgroups in order to be able to determine with certainty if your Y or mtDNA ancestor was Native.

And herein lies the rub.

Certainty is relative, pardon the pun.

We know unquestionably that some haplogroups, as defined by Y SNPs and mtDNA full sequence testing, ARE Native, and we know that some haplogroups have never (to date) been found in a Native population, but there are other haplogroup subgroups that are ambiguous and are either found in both Asia/Europe and the Americas, or their origin is uncertain. One by one, as more people test and we obtain additional data, we solve these mysteries.

Let’s look at a recent example.

Haplogroup X2b4

Haplogroup X2b4 was found in the descendants of Radegonde Lambert, an Acadian woman born sometime in the 1620s and found in Acadia (present day Nova Scotia) married to Jean Blanchard as an adult. It was widely believed that she was the daughter of Jean Lambert and his Native wife. However, some years later, a conflicting record arose in which the husband of Radegonde’s great-granddaughter gave a deposition in which he stated that Radegonde came from France with her husband.

Which scenario was true? For years, no one else tested with haplogroup X2b4 that had any information as to the genesis of their ancestors, although several participants tested who descended from Radegonde.

Finally, in 2016, we were able to solve this mystery once and for all. I had formed the X2b4 project with Marie Rundquist and Tom Glad, hoping to attract people with haplogroup X2b4. Two pivotal events happened.

  • Additional people tested at Family Tree DNA and joined the X2b4 project.
  • Genographic Project records became available to me as an affiliate researcher.

At Family Tree DNA, we found other occurrences of X2b4 in:

  • The Czech Republic
  • Devon in the UK
  • Birmingham in the UK

Was it possible that X2b4 could be both European and Native, meaning that some descendants had migrated east and crossed the Beringia land bridge, and some has migrated westward into Europe?

Dr. Doron Behar in the supplement to his publication, “A Copernican” Reassessment of the Human Mitochondrial DNA Tree from its Root” provides the creation dates for haplogroup X through X2b4 as follows:

native-mt-x2b4

These dates would read 31,718 years ago plus or minus 11,709 (eliminating the numbers after the decimal point) which would give us a range for the birth of haplogroup X from 43,427 years ago to 20,009 years ago, with 31,718 being the most likely date.

Given that X2b4 was “born” between 2,992 and 8,186 years ago, the answer has to be no, X2b4 cannot be found both in the Native population and European population since at the oldest date, 8,100 years ago, the Native people had already been in the Americas between 2,000 and 18,000 years.

Of course, all kinds of speculation could be (and has been) offered, about Native people being taken to Europe, although that speculation is a tad bit difficult to rationalize in the Czech Republic.

The next logical question is if there are documented instances of X2b4 in the Native population in the Americas?

I turned to the Genographic Project where I found no instances of X2b4 in the Native population and the following instances of X2b4 in Europe.

  • Ireland
  • Czech
  • Serbia
  • Germany (6)
  • France (2)
  • Denmark
  • Switzerland
  • Russia
  • Warsaw, Poland
  • Norway
  • Romania
  • England (2)
  • Slovakia
  • Scotland (2)

The conclusion relative to X2b4 is clearly that X2b4 is European, and not aboriginally Native.

The Genographic Project Data Base

As a researcher, I was absolutely thrilled to have access to another 700,000+ results, over 475,000 of which are mitochondrial.

The Genographic Project tests people whose identity remains anonymous. One of the benefits to researchers is that individuals in the public participation portion of the project can contribute their own information anonymously for research by answering a series of questions.

I was very pleased to see that one of the questions asked is the location of the birth of the participant’s most distant matrilineal ancestor.

Tabulation and analysis should be a piece of cake, right? Just look at that “most distant ancestor” response, or better yet, utilize the Genographic data base search features, sort, count, and there you go…

Well, guess again, because one trait that is universal, apparently, between people is that they don’t follow instructions well, if at all.

The Genographic Project, whether by design or happy accident, has safeguards built in, to some extent, because they ask respondents for the same or similar information in a number of ways. In any case, this technique provides researchers multiple opportunities to either obtain the answer directly or to put 2+2 together in order to obtain the answer indirectly.

Individuals are identified in the data base by an assigned numeric ID. Fields that provide information that could be relevant to ascertaining mitochondrial ethnicity and ancestral location are:

native-mt-geno-categories

I utilized these fields in reverse order, giving preference to the earliest maternal ancestor (green) fields first, then maternal grandmother (teal), then mother (yellow), then the tester’s place of birth (grey) supplemented by their location, language and ethnicity if applicable.

Since I was looking for very specific information, such as information that would tell me directly or suggest that the participant was or could be Native, versus someone who very clearly wasn’t, this approach was quite useful.

It also allowed me to compare answers to make sure they made sense. In some cases, people obviously confused answers or didn’t understand the questions, because the three earliest ancestor answers cannot contain information that directly contradict each other. For example, the earliest ancestor place of birth cannot be Ireland and the language be German and the ethnicity be Cherokee. In situations like this, I omitted the entire record from the results because there was no reliable way to resolve the conflicting information.

In other cases, it was obvious that if the maternal grandmother and mother and tester were all born in China, that their earliest maternal ancestor was not very likely to be Native American, so I counted that answer as “China” even though the respondent did not directly answer the earliest maternal ancestor questions.

Unfortunately, that means that every response had to be individually evaluated and tabulated. There was no sort and go! The analysis took several weeks in the fall of 2016.

By Haplogroup – Master and Summary Tables

For each sub-haplogroup, I compiled, minimally, the following information shown as an example for haplogroup A with no subgroup:

native-mt-master-chart

The “Previously Proven Native” link is to my article titled Native American Mitochondrial Haplogroups where I maintain an updated list of haplogroups proven or suspected Native, along with the source(s), generally academic papers, for that information.

In some cases, to resolve ambiguity if any remained, I also referenced Phylotree, mtDNA Community and/or GenBank.

For each haplogroup or subgroup within haplogroup, I evaluated and listed the locations for the Genographic “earliest maternal ancestor place of birth” locations, but in the case of the haplogroup A example above, with 4198 responses, the results did not fit into the field so I added the information as supplemental.

By analyzing this information after completing a master tablet for each major haplogroup and subgroups, meaning A, B, C, D and X, I created summary tables provided in the haplogroup sections in this paper.

Family Tree DNA Projects

Another source of haplogroup information is the various mitochondrial DNA projects at Family Tree DNA.

Each project is managed differently, by volunteers, and displays or includes different information publicly. While different information displayed and lack of standardization does present challenges, there is still valuable information available from the public webpages for each mitochondrial haplogroup referenced.

Challenges

The first challenge is haplogroup naming. For those “old enough” to remember when Y DNA haplogroups used to be called by names such as R1b1c and then R1b1a2, as opposed to the current R-M269 – mitochondrial DNA is having the same issue. In other words, when a new branch needs to be added to the tree, or an entire branch needs to be moved someplace else, the haplogroup names can and do change.

In October and November 2016 when I extracted Genographic project data, Family Tree DNA was on Phylotree version 14 and the Genographic Project was on version 16. The information provided in various academic papers often references earlier versions of the phylotree, and the papers seldom indicate which phylotree version they are using. Phylotree is the official name for the mitochondrial DNA haplogroup tree.

Generally, between Phylotree versions, the haplogroup versions, meaning names, such as A1a, remain fairly consistent and the majority of the changes are refinements in haplogroup names where subgroups are added and all or part of A1a becomes A1a1 or A1a2, for example. However, that’s not always true. When new versions are released, some haplogroup names remain entirely unchanged (A1a), some people fall into updated haplogroups as in the example above, and some find themselves in entirely different haplogroups, generally within the same main haplogroup. For example, in Phylotree version 17, all of haplogroup A4 is obsoleted, renamed and shifted elsewhere in the haplogroup A tree.

The good news is that both Family Tree DNA and the Genographic project plan to update to Phylotree V17 in 2017. After that occurs, I plan to “equalize” the results, hopefully “upgrading” the information from academic papers to current haplogroup terminology as well if the authors provided us with the information as to the haplogroup defining mutations that they utilized at publication along with the entire list of sample mutations.

A second challenge is that not all haplogroup projects are created equal. In fact, some are entirely closed to the public, although I have no idea why a haplogroup project would be closed. Other projects show only the map. Some show surnames but not the oldest ancestor or location. There was no consistency between projects, so the project information is clearly incomplete, although I utilized both the public project pages and maps together to compile as much information as possible.

A third challenge is that not every participant enters their most distant ancestor (correctly) nor their ancestral location, which reduces the relevance of results, whether inside of projects, meaning matches to individual testers, or outside of projects.

A fourth challenge is that not every participant enables public project sharing nor do they allow the project administrators to view their coding region results, which makes participant classification within projects difficult and often impossible.

A fifth challenge is that in Family Tree DNA mitochondrial projects, not everyone has tested to the full sequence level, so some people who are noted as base haplogroup “A,” for example, would have a more fully defined haplogroup is they tested further. On the other hand, for some people, haplogroup A is their complete haplogroup designation, so not all designations of haplogroup A are created equal.

A sixth challenge is that in the Genographic Project, everyone has been tested via probes, meaning that haplogroup defining mutation locations are tested to determine full haplogroups, but not all mitochondrial locations are not tested. This removes the possibility of defining additional haplogroups by grouping participants by common mutations outside of haplogroup defining mutations.

A seventh challenge is that some resources for mitochondrial DNA list haplogroup mutations utilizing the CRS (Cambridge Reference Sequence) model and some utilize the RSRS (Reconstructed Sapiens Reference Sequence) model, meaning that the information needs to be converted to be useful.

Resources

Let’s look at the resources available for each resource type utilized to gather information.

native-mt-resources

The table above summarizes the differences between the various sources of information regarding mitochondrial haplogroups.

Before we look at each Native American haplogroup, let’s look at common myths, family stories and what constitutes proof of Native ancestry.

Family Stories

In the US, especially in families with roots in Appalachia, many families have the “Cherokee” or “Indian Princess” story. The oral history is often that “grandma” was an “Indian princess” and most often, Cherokee as well. That was universally the story in my family, and although it wasn’t grandma, it was great-grandma and every single line of the family carried this same story. The trouble was, it proved to be untrue.

Not only did the mitochondrial DNA disprove this story, the genealogy also disproved it, once I stopped looking frantically for any hint of this family line on the Cherokee rolls and started following where the genealogy research indicated. Now, of course this isn’t to say there is no Native IN that line, but it is to say that great-grandma’s direct matrilineal (mitochondrial) line is NOT Native as the family story suggests. Of course family stories can be misconstrued, mis-repeated and embellished, intentionally or otherwise with retelling.

Family stories and myths are often cherished, having been handed down for generations, and die hard.

In fact, today, some unscrupulous individuals attempt to utilize the family myths of those who “self-identify” their ancestor as “Cherokee” and present the myths and resulting non-Native DNA haplogrouip results as evidence that European and African haplogroups are Native American. Utilizing this methodology, they confirm, of course, that everyone with a myth and a European/African haplogroup is really Native after all!

As the project administrator of several projects including the American Indian and Cherokee projects, I can tell you that I have yet to find anyone who has a documented, as in proven lineage, to a Native tribe on a matrilineal line that does not have a Native American haplogroup. However, it’s going to happen one day, because adoptions of females into tribes did occur, and those adopted females were considered to be full tribal members. In this circumstance, your ancestor would be considered a tribal member, even if their DNA was not Native.

Given the Native tribal adoption culture, tribal membership of an individual who has a non-Native haplogroup would not be proof that the haplogroup itself was aboriginally Native – meaning came from Asia with the other Native people and not from Europe or Africa with post-Columbus contact. However, documenting tribal membership and generational connectivity via proven documentation for every generation between that tribally enrolled ancestor and the tester would be a first step in consideration of other haplogroups as potentially Native.

In Canada, the typical story is French-Canadian or metis, although that’s often not a myth and can often be proven true. We rely on the mtDNA in conjunction with other records to indicate whether or not the direct matrilineal ancestor was French/European or aboriginal Canadian.

In Mexico, the Caribbean and points south, “Spain” in the prevalent family story, probably because the surnames are predominantly Spanish, even when the mtDNA very clearly says “Native.” Many family legends also include the Canary Islands, a stopping point in the journey from Europe to the Caribbean.

Cultural Pressures

It’s worth noting that culturally there were benefits in the US to being Native (as opposed to mixed blood African) and sometimes as opposed to entirely white. Specifically, the Native people received head-right land payments in the 1890s and early 1900s if they could prove tribal descent by blood. Tribal lands, specifically those in Oklahoma owned by the 5 Civilized Tribes (Cherokee, Choctaw, Chickasaw, Creek and Seminole) which had been previously held by the tribe were to be divided and allotted to individual tribal members and could then be sold. Suddenly, many families “remembered” that they were of Native descent, whether they were or not.

Culturally and socially, there may have been benefits to being Spanish over Native in some areas as well.

It’s also easy to see how one could assume that Spain was the genesis of the family if Spanish was the spoken language – so care had to be exercised when interpreting some Genographic answers. Chinese can be interpreted to mean “China” or at least Asia, meaning, in this case, “not Native,” but Spanish in Mexico or south of the US cannot be interpreted to mean Spain without other correlating information.

Language does not (always) equal origins. Speaking English does not mean your ancestors came from England, speaking Spanish does not mean your ancestors came from Spain and speaking French does not mean your ancestors came from France.

However, if your ancestors lived in a country where the predominant language was English, Spanish or French, and your ancestor lived in a location with other Native people and spoke a Native language or dialect, that’s a very compelling piece of evidence – especially in conjunction with a Native DNA haplogroup.

What Constitutes Proof?

What academic papers use as “proof” of Native ancestry varies widely. In many cases, the researchers don’t make a case for what they use as proof, they simply state that they had one instance of A2x from Mexico, for example. In other cases, they include tribal information, if known. When stated in the papers, I’ve included that information on the Native American Mitochondrial Haplogroups page.

Methodology

I have adopted a similar methodology, tempered by the “guilt by genetic association” guideline, keeping in mind that both FTDNA projects and Genographic project public participants all provide their own genealogy and self-identify. In other words, no researcher traveled to Guatemala and took a cheek swab or blood sample. The academic samples and samples taken by the Genographic Project in the field are not included in the Genographic public data base available to researchers.

However, if the participant and their ancestors noted were all born in Guatemala, there is no reason to doubt that their ancestors were also found in the Guatemala region.

Unfortunately, not everything was that straightforward.

Examples:

  • If there were multiple data base results as subsets of base haplogroups previously known to be Native from Mexico and none from anyplace else in the world, I’m comfortable calling the results “Native.”
  • If there are 3 results from Mexico, and 10 from Europe, especially if the European results are NOT from Spain or Portugal, I’m NOT comfortable identifying that haplogroup as Native. I would identify it as European so long as the oldest date in the date ranges identifying when the haplogroup was born is AFTER the youngest migration date. For example, if the haplogroup was born 5,000 years ago and the last known Beringia migration date is 10,000 years ago, people with the same haplogroup cannot be found both in Europe and the Americas indigenously. If the haplogroup birth date is 20,000 years ago and the migration date is 10,000 years ago, clearly the haplogroup CAN potentially be found on both continents as indigenous.
  • In some cases, we have the reverse situation where the majority of results are from south of the US border, but one or two claim Spanish or Portuguese ancestry, which I suspect is incorrect. In this case, I will call the results Native so long as there are a significant number of results that do NOT claim Spanish or Portuguese ancestry AND none of the actual testers were born in Spain or Portugal.
  • In a few cases, the FTDNA project and/or Genographic data refute or at least challenge previous data from academic papers. Future information may do the same with this information today, especially where the data sample is small.

Because of ambiguity, in the master data table (not provided in this paper) for each base haplogroup, I have listed every one of the sub-haplogroups and all the locations for the oldest ancestors, plus any other information provided when relevant in the actual extracted data.

When in doubt, I have NOT counted a result as Native. When the data itself is questionable or unreliable, I removed the result from the data and count entirely.

I intentionally included all of the information, Native and non-Native, in my master extracted data tables so that others can judge for themselves, although I am only providing summary tables here. Detailed information will be provided in a series of articles or in an academic paper after both the Family Tree DNA data base and the Genographic data base are upgraded to Phylotree V17.

The Haplogroup Summary Table

The summary table format used for each haplogroup includes the following columns and labels:

  • Hap = Haplogroup as listed at Family Tree DNA, in academic papers and in the Genographic project.
  • Previous Academic Proven = Previously proven or cited as Native American, generally in Academic papers. A list of these haplogroups and papers is provided in the article, Native American Mitochondrial Haplogroups.
  • Academic Confirmed = Academic paper haplogroup assignments confirmed by the Genographic Project and/or Family Tree DNA Projects.
  • Previous Suspected = Not academically proven or cited at Native, but suspected through any number of sources. The reasons each haplogroup is suspected is also noted in the article, Native American Mitochondrial DNA Haplogroups.
  • Suspected Confirmed = Suspected Native haplogroups confirmed as Native.
  • FTDNA Project Proven = Mitochondrial haplogroup proven or confirmed through FTDNA project(s).
  • Geno Confirmed = Mitochondrial haplogroup proven or confirmed through the Genographic Project data base.

Color Legend:

native-mt-color-legend

Additional Information:

  • Possibly, probably or uncertain indicates that the data is not clear on whether the haplogroup is Native and additional results are needed before a definitive assignment is made.
  • No data means that there was no data for this haplogroup through this source.
  • Hap not listed means that the original haplogroup is not listed in the Genographic data base indicating the original haplogroup has been obsoleted and the haplogroup has been renamed.

The following table shows only the A haplogroups that have now been proven Native, omitting haplogroups proven not to be Native through this process, although the original master data table (not included here) includes all information extracted including for haplogroups that are not Native. Summary tables show only Native or potentially Native results.

Let’s look at the summary results grouped by major haplogroup.

Haplogroup A

Haplogroup A is the largest Native American haplogroup.

native-mt-hap-a-pie

More than 43% of the individuals who carry Native American mitochondrial DNA fall into a subgroup of A.

Like the other Native American haplogroups, the base haplogroup was formed in Asia.

Family Tree DNA individual participant pages provide participants with both a Haplogroup Frequency Map, shown above, and a Haplogroup Migration Map, shown below.

native-mt-migration

The Genographic project provides heat maps showing the distribution of major haplogroups on a continental level. You can see that, according to this heat map from when the Genographic Project was created, the majority of haplogroup A is found in the northern portion of the Americas.

native-mt-hap-a-heat

Additionally, the Genographic Project data base also provides a nice tree structure for each haplogroup, beginning with Mitochondrial Eve, in Africa, noted as the root, and progressing to the current day haplogroups.

native-mt-hap-a-tree-root

native-mt-hap-a-tree

Haplogroup A Projects

I enjoy the added benefit of being one of the administrators, along with Marie Rundquist, of the haplogroup A project at Family Tree DNA, as well as the A10, A2 and A4 projects. However, in this paper, I only included information available on the projects’ public pages and not information participants sent to the administrators privately.

The Haplogroup A Project at Family Tree DNA is a public project, meaning available for anyone with haplogroup A to join, and fully publicly viewable with the exception of the participant’s surname, since that is meaningless when the surname traditionally changes with every generation. However, both the results, complete with the Maternal Ancestor Name, and the map, are visible. HVR1 and HVR2 results are displayed, but coding region results are never available to be shown in projects, by design.

native-mt-hap-a-project

The map below shows all participants for the entire project who have entered a geographic location. The three markers in the Middle East appear to be mis-located, a result of erroneous user geographic location input. The geographic locations are selected by participants indicating the location of their most distant mitochondrial ancestor. All 3 are Spanish surnames and one is supposed to be in Mexico. Please disregard those 3 Middle Eastern pins on the map below.

native-mt-hap-a-project-map

Haplogroup A Summary Table

The subgroups of haplogroup A and the resulting summary data are shown in the table below.

native-mt-hap-a-chart-1

native-mt-hap-a-chart-2

native-mt-hap-a-chart-3

  • Total haplogroups Native – 75
  • Total haplogroups uncertain – 1
  • Total haplogroups probable – 1
  • Total new Native haplogroups – 38, 1 probable.
  • Total new Native haplogroups proven by FTDNA Projects – 9, 1 possibly
  • Total new Native haplogroups proven by Genographic Project – 35, 1 probable

Haplogroup B

Haplogroup B is the second largest Native American haplogroup, with 23.53% of Native participants falling into this haplogroup.

native-mt-hap-b-pie

The Genographic project provides the following heat map for haplogroup B4, which includes B2, the primary Native subgroup.

native-mt-hap-b-heat

The haplogroup B tree looks like this:

native-mt-hap-b-tree-root

native-mt-hap-b-tree

native-mt-hap-b-tree-2

B4 and B5 are main branches.

You will note below that B2 falls underneath B4b.

native-mt-hap-b-tree-3

Haplogroup B Projects

At Family Tree DNA, there is no haplogroup B project, but there is a haplogroup B2 project, which is where the majority of the Native results fall. Haplogroup B Project administrators have included a full project display, along with a map. All of the project participants are shown on the map below.

native-mt-hap-b-project-map

Please note that the pins colored other than violet (haplogroup B) should not be shown in this project. Only haplogroup B pins are violet.

Haplogroup B Summary Table

native-mt-hap-b-chart-1

native-mt-hap-b-chart-2

  • Total haplogroups Native – 63
  • Total haplogroups refuted – 1
  • Total new Native haplogroups – 43
  • Total new Native haplogroups proven by Family Tree DNA projects – 12
  • Total new Native haplogroups proven by Genographic Project – 41

Haplogroup C

Haplogroup C is the third largest Native haplogroup with 22.99% of the Native population falling into this haplogroup.

native-mt-hap-c-pie

Haplogroup C is primarily found in Asia per the Genographic heat map.

native-mt-hap-c-heat

The haplogroup C tree is as follows:

native-mt-hap-c-root

native-mt-hap-c-tree-1

native-mt-hap-c-tree-2

Haplogroup C Project

Unfortunately, at Family Tree DNA, the haplogroup C project has not enabled their project pages, even for project members.

When I first began compiling this data, the Haplogroup C project map was viewable.

native-mt-hap-c-project-map-world

Haplogroup C Summary Table

native-mt-hap-c-chart-1

native-mt-hap-c-chart-2

  • Total haplogroups Native – 61
  • Total haplogroups refuted – 2
  • Total haplogroups possible – 1
  • Total haplogroups probable – 1
  • Total new Native haplogroups – 8
  • Total new Native haplogroups proven by Family Tree DNA projects – 6
  • Total new Native haplogroups proven by Genographic Project – 5, 1 possible, 1 probable

Haplogroup D

Haplogroup D is the 4th largest, or 2nd smallest Native haplogroup, depending on your point of view, with 6.38% of Native participants falling into this haplogroup.

native-mt-hap-d-pie

Haplogroup D is found throughout Asia, into Europe and throughout the Americas.

native-mt-hap-d-heat

Haplogroups D1 and D2 are the two subgroups primarily found in the New World.

native-mt-hap-d-heat-d1

The haplogroup D1 heat map is shown above and D2 is shown below.

native-mt-hap-d-heat-d2

The Tree for haplogroup D is a subset of M.

native-mt-hap-d-tree-root

Haplogroup D begins as a subhaplogroup of M80..

native-mt-hap-d-tree-2

Haplogroup D Projects

D is publicly viewable, but shows testers last name, no ancestor information and no location, so I utilized maps once again.

native-mt-hap-d-project-map

Haplogroup D Summary Table

native-hap-d-chart-1

native-hap-d-chart-2

  • Total haplogroups Native – 50
  • Total haplogroups possibly both – 3
  • Total haplogroups uncertain – 2
  • Total haplogroups probable – 1
  • Total haplogroups refuted – 3
  • Total new Native Haplogroups – 25
  • Total new Native haplogroups proven by Family Tree DNA projects – 2
  • Total new Native haplogroups proven by Genographic Project – 22, 1 probably

Haplogroup X

Haplogroup X is the smallest of the known Native base haplogroups.

native-mt-hap-x-pie

Just over 3% of the Native population falls into haplogroup X.

The heat map for haplogroup X looks very different than haplogroups A-D.

native-mt-hap-x-heat

The tree for haplogroup X shows that it too is also a subgroup of M and N.

native-mt-hap-x-root

native-mt-hap-x-tree

Haplogroup X Project

At Family Tree DNA, the Haplogroup X project is visible, but with no ancestral locations displayed. I utilized the map, which was visible.

native-mt-hap-x-project-map

This map of the entire haplogroup X project tells you immediately that the migration route for Native X was not primarily southward, but east. Haplogroup X is found primarily in the US and in the eastern half of Canada.

Haplogroup X Summary Table

native-mt-hap-x-chart

  • Total haplogroups Native – 10
  • Total haplogroups uncertain, possible or possible both Native and other – 8
  • Total New Native haplogroups – 0

Haplogroup M

Haplogroup M, a very large, old haplogroup with many subgroups, is not typically considered a Native haplogroup.

The Genographic project shows the following heat map for haplogroup M.

native-mt-hap-m-heat

The heat map for haplogroup M includes both North and South America, but according to Dr. Miguel Vilar, Science Manager for the Genographic Project, this is because both haplogroups C and D are subsets of M.

native-mt-hap-m-migration

The haplogroup M migration map from the Genographic Project shows haplogroup M expanding across southern Asia.

native-mt-hap-m-root

The tree for haplogroup M, above, is abbreviated, without the various subgroups being expanded.

native-mt-hap-m1-tree

The M1 and M1a1e haplogroups shown above are discussed in the following section, as is M18b, below.

native-mt-hap-m18b-tree

The Haplogroup M Project

The haplogroup M project at Family Tree DNA shows the worldwide presence of haplogroup M and subgroups.

native-mt-hap-m-project-map

Native Presence

Haplogroup M was originally reported in two Native burials in the Americas. Dr. Ripan Malhi reported haplogroup M (excluding M7, M8 and M9) from two separate skeletons from the same burial in China Lake, British Columbia, Canada, about 150 miles north of the Washington State border, dating from about 5000 years ago. Both skeletons were sequenced separately in 2007, with identical results and are believed to be related.

While some researchers are suspicious of these findings as being incomplete, a subsequent paper in 2013, Ancient DNA-Analysis of Mid-Holocene Individuals from the Northwest Coast of North America Reveals Different Evolutionary Paths for Mitogenomes, which included Mahli as a co-author states the following:

Two individuals from China Lake, British Columbia, found in the same burial with a radiocarbon date of 4950+/−170 years BP were determined to belong to a form of macrohaplogroup M that has yet to be identified in any extant Native American population [24], [26]. The China Lake study suggests that individuals in the early to mid-Holocene may exhibit mitogenomes that have since gone extinct in a specific geographic region or in all of the Americas.

Haplogroup M Summary Table

native-mt-hap-m-chart

One additional source for haplogroup M was found in GenBank noted as M1a1e “USA”, but there were also several Eurasian submissions for M1a1e as well. However, Doron Behar’s dates for M1a1e indicate that the haplogroup was born about 9,813 years ago, plus or minus 4,022 years, giving it a range of 5,971 to 13,835 years ago, meaning that M1a1e could reasonably be found in both Asia and the Americas. There were no Genographic results for M1a1e. At this point, M1a1e cannot be classified as Native, but remains on the radar.

Hapologroup M1 was founded 23,679 years ago +-4377 years. It is found in the Genographic Project in Cuba, Venezuela and is noted as Native in the Midwest US. M1 is also found in Colorado and Missouri in the haplogroup M project at Family Tree DNA, but the individuals did not have full sequence tests nor was additional family information available in the public project.

The following information is from the master data table for haplogroup M potentially Native haplogroups.

Haplogroup M Master Data Table for Potentially Native Haplogroups

The complete master data tables includes all subhaplogroups of M, the partial table below show only the Native haplogroups.

native-mt-hap-m-chart-1

native-mt-hap-m-master-data-chart-2

Haplogroup M18b is somewhat different in that two individuals with this haplogroup at Family Tree DNA have no other matches.  They both have a proven connection to Native families from interrelated regions in North Carolina.

I initiated communications with both individuals who tested at Family Tree DNA who subsequently provided their genealogical information. Both family histories reach back into the late 1700s, one in the location where the Waccamaw were shown on maps in in the early 1700s, and one near the border of Virginia and NC. One participant is a member of the Waccamaw tribe today. A family migration pattern exists between the NC/VA border region and families to the Waccamaw region as well. An affidavit exists wherein the family of the individual from the NC/VA border region is sworn to be “mixed” but with no negro blood.

In summary:

  • Haplogroups M and M1 could easily be both Native as well as Asian/European, given the birth age of the haplogroup.
  • Haplogroup M1a1e needs additional results.
  • Haplogroup M18b appears to be Native, but could also be found elsewhere given the range of the haplogroup birth age. Additional proven Native results could bolster this evidence.
  • In addition to the two individuals with ancestors from North Carolina, M18b is also reported in a Sioux individuals with mixed race ethnicity

The Dark Horse Late Arrival – Haplogroup F

I debated whether I should include this information, because it’s tenuous at best.

The American Indian project at Family Tree DNA includes a sample of F1a1 full sequence result whose most distant matrilineal ancestor is found in Mexico.

Haplogroup F is an Asian haplogroup, not found in Europe or in the Americas.

native-mt-hap-f-heat

native-mt-hap-f-migration

Haplogroup F, according to the Genographic Project, expands across central and southern Asia.

native-mt-hap-f-root

native-mt-hap-f1a1-tree

According to Doron Behar, F1a1 was born about 10,863 years ago +- 2990 years, giving it a range of 7,873 – 13,853.

Is this Mexican F1a1 family Native? If not, how did F1a1 arrive in Mexico, and when? F1a1 is not found in either Europe or Africa.

In August, 2015, an article published in Science, Genomic evidence for the Pleistocene and recent population history of Native Americans by Raghaven et al suggested that a secondary migration occurred from further south in Asia, specifically the Australo-Melanesians, as shown in the diagram below from the paper. If accurate, this East Asian migration originating further south could explain both the haplogroup M and F results.

native-mt-nature-map

A second paper, published in Nature in September 2015 titled Genetic evidence for two founding populations of the Americas by Skoglund et al says that South Americans share ancestry with Australasian populations that is not seen in Mesoamericans or North Americans.

The Genographic project has no results for F1a1 outside of Asia.

I have not yet extracted the balance of haplogroup F in the Genographic project to look for other indications of haplogroups that could potentially be Native.

Haplogroup F Project

The haplogroup F project at Family Tree DNA shows no participants in the Americas, but several in Asia, as far south as Indonesia and also into southern Europe and Russia.

native-mt-hap-f-project-map

Haplogroup F Summary Table

native-mt-hap-f-chart

Haplogroup F1a1 deserves additional attention as more people test and additional samples become available.

Native Mitochondrial Haplogroup Summary

Research in partnership with the Genographic Project as well as the publicly available portions of the projects at Family Tree DNA has been very productive. In total, we now have 259 proven Native haplogroups. This research project has identified 114 new Native haplogroups, or 44% of the total known haplogroups being newly discovered within the Genographic Project and the Family Tree DNA projects.

native-mt-hap-summary

Acknowledgements

800 Articles Strong

800-strong

Today is something of a red-letter day. This is the 801st article published on this blog.

This blog, DNA-Explained, was christened on July 11, 2012 and will soon be 5 years old, as hard as that is to believe. In some ways, it feels like this blog has been around “forever” and in other ways, it feels like it’s very new, because there is always some interesting topic to write about.

Truthfully, I can’t believe I’ve written 800 articles. No wonder some of the letters are worn off of my keyboard. And it’s my second keyboard!

My original goal was one article per week, which would have been about 235 articles by now. I wasn’t sure I could accomplish that. It’s amazing what inspiration can do! I love genetic genealogy every bit as much today as I did then, if not more. What an incredibly exciting time to be alive with an unbelievable opportunity to participate in an unfolding field with new discoveries being made on an almost daily basis.

I had been considering a DNA blog when Spencer Wells, then Scientist in Residence at the National Geographic Society, suggested that I SHOULD author a blog. That encouragement was all it took to motivate me. Thanks so much Spencer for that final nudge!!!

spencer and me

Just 12 days after DNA-Explained’s launch, the Genographic 2.0 product was introduced and I was privileged to participate in that announcement.

I started writing articles in self-defense, truthfully, because I was receiving the same questions over and over again. I figured if I could write the answer once, I could then just point the next person with that same question to an answer that included graphics and illustrations and was a much better answer than I could provide in an e-mail.

Plus, repetitively recreating the same answer was a time-waster – and blogging to share publicly with the goal of helping lots of people learn seemed the perfect solution.

I had no idea, and I mean none, that DNA testing in the direct to consumer marketplace would explode like it has. I’m glad I started writing when I did, because there are ever-more people asking questions. That’s a good thing, because it means people are testing and learning what messages their DNA has for them.

Our DNA is the most personal record of our ancestors that we’ll ever have – and today more and more tools exist to interpret what those ancestors are telling us. We are still panning for gold on the frontier of science although we know infinitely more than we did a decade or 5 years ago, and we know less than we will 5 or 10 years from now. We are still learning every single day. That’s what makes this field so exciting, and infinitely personal.

Here’s part of what I said in my introductory article:

Genetic genealogy is a world full of promise, but it changes rapidly and can be confusing. People need to understand how to use the numerous tools available to us to unravel our ancestral history.

People also love to share stories. We become inspired by the successes of others, and ideas are often forthcoming that we would not have otherwise thought of.

In light of that, I’ve tried to include a wide variety of articles at every level so that there is something for everyone. I hope I’ve managed to make genetics interesting and shared some of my enthusiasm with you over the years.

In Celebration

To celebrate this 800 article-versary, I’m going to share a few things.

  • Article organization and how to find what you want
  • The 10 most popular articles of those 800
  • Two things people can do to help themselves
  • Articles I wish people would read
  • Questions asked most frequently

Then, I’m going to ask you what you’d like for me to write about in the future.

Articles Organization aka How To Find What You Want

Blogs allow you to group articles by both categories and tags, two ways of organizing your articles so that people and search engines can find them.

Each article is identified by categories. You can click on any of the categories, below, to see which articles fall into that category. These are also some of the keywords for the blog search feature.

I’ve also grouped articles by tags as shown on the sidebar of the blog. The larger text indicates tags with more articles.

800-tags

You can click on any of those as well (on the actual blog page) to view all the articles that fall into that tag group.

For example, one of my 52 Ancestor Stories would be tagged with “52 Weeks of Ancestors” but if it discussed Y DNA, that would be one of the categories selected.

At the end of every blog article, you can see the category or categories the article is posted under, tags and other pertinent information about that article.

800-end-of-article

The Top 10 Articles

  1. Proving Native American Ancestry Using DNA
  2. 4 Kinds of DNA for Genetic Genealogy
  3. Ethnicity Results – True or Not?
  4. Mythbusting – Women, Fathers and DNA
  5. Genealogy and Ethnicity DNA Testing – 3 Legitimate Companies
  6. How Much Indian Do I Have in Me???
  7. What is a Haplogroup?
  8. Thick Hair, Small Boobs, Shovel Shaped Teeth and More
  9. Ethnicity Testing and Results
  10. 23andMe, Ancestry and Selling Your DNA Information

The Two Things People Can Do To Help Themselves

  1. Search first.

Before asking a question, I wish people would try searching my blog for the answer. Using the search box in the upper right hand corner, the blog is fully key word searchable.

800-search

Furthermore, even if you can’t figure out the right key word to search, you can also find articles on my blog by searching for phrases using google.

2. Upload GEDCOM files.

Your DNA testing is only as good as the comparisons you can make, and the ancestors and ancestral links you can find. Please, please, PLEASE upload GEDCOM files to Family Tree DNA and GedMatch. If you don’t have a tree, you can create one at Family Tree DNA. Link your tree to your DNA results on Ancestry and share your results. 23andMe has no tree ability at this time.

The Articles I Wish People Would Read

In addition to some of the articles already listed in the top 10, I wish people would read:

Questions Asked Most Frequently

  • Questions relating to Native American heritage and testing.
  • Questions relating to ethnicity, especially when the results are unexpected or don’t seem to align with what is known or family oral history.
  • Overwhelmed newbies who receive results and don’t have any idea how to interpret what they’ve received, which is why I created the Help page.

The Future – What Articles Would You Like to See?

It’s your turn.

What topics would you like to see me cover in upcoming articles? Is there something in particular that you find confusing, or enticing, or exciting?

I’m not promising that I’ll write about every topic, and some may be combined, but articles are often prompted by questions and suggestions from readers.

And speaking of readers…

Thank You

A very big thank you to all of my subscribers and followers for making DNA-Explained so popular and such a success. You folks are amazing, infinitely giving and helpful. We really are a community!

thank-you

 

Native American and First Nations DNA Testing – Buyer Beware

Native DNA in Feathers

This week, a woman in North Carolina revealed that she descends from the extinct Beothuk tribe in Canada as a result of a DNA test from a Canadian DNA testing company. This has caused quite an uproar, in both genetic genealogy and Native American research communities, and has been resoundingly discredited by geneticists.

People’s motivation for wanting to know if they have Native heritage generally falls into the following categories:

  • Curiosity and a desire to confirm a family story
  • Desire to recover lost heritage
  • Desire to identify or join a tribe
  • Desire to obtain services provided to eligible tribal members, such as educational benefits
  • Desire to obtain benefits provided to eligible tribal members, such as a share of casino profits

Questions about DNA testing to reveal Native ancestry are the most common questions I receive and my Native DNA articles are the most visited on my website and blog.

Legitimate DNA Tests for Native Heritage

There are completely legitimate tests for Native ancestry, including the Y DNA and mitochondrial DNA tests for direct paternal (blue box genealogy line, below) and direct matrilineal lines (red circle genealogy line, below). Both Y and mitochondrial DNA have scientifically identified and confirmed haplogroups found only in Native Americans, as discussed in this article. Both Y and mitochondrial DNA at appropriate testing levels can identify a Native ancestor back in time thousands of years.

Y and mito

However, if the Native ancestor does not descend from the direct paternal or direct matrilineal lines, the only DNA test left is an autosomal test which tests all of your ancestral lines, but which can only reliably identify ancestral heritage for the past 5 or 6 generations in any of those lines due to recombination of DNA with the other parent in each generation. Autosomal tests provide you with percentage estimates of your ethnicity although they can vary widely between companies for various reasons. All three of these tests are available from Family Tree DNA as part of their normal product offering.

If you’d like to see an example of genealogy research combined with all three types of DNA testing for a Native Sioux man, please read about John Iron Moccasin.

Less Than Ethical DNA Tests for Native Heritage

Because of the desire within the consuming public to know more about their Native heritage, several specialty testing services have emerged to offer “Native American” tests. Recently, one, Accu-Metrics out of Canada has been highly criticized in the media for informing a woman that she was related to or descended from the extinct Beothuk tribe based on a match to a partial, damaged, mitochondrial sample from skeletal remains, now in housed in Scotland.

When you look at some of these sites, they spend a lot of time convincing you about the qualifications of the lab they use, but the real problem is not with the laboratory, but their interpretation of what those results mean to their clients – e.g. Beothuk.

Those of us who focus on Native American ancestry know unequivocally that “matching” someone with Native ancestry does NOT equate to being from that same tribe. In fact, we have people in the American Indian Project and various Native haplogroup projects who match each other with either Native Y or mitochondrial results who are tribally enrolled or descended from tribes from very different parts of the Americas, as far distant as Canada and South America.

Based on this 2007 paper, A Preliminary Analysis of the DNA and Diet of the Extinct Beothuk: A Systematic Approach to Ancient Human DNA, describing the analysis of the Beothuk remains, it appears that only the HVR1 region of the Beothuk skeletal remains were able to be partially sequenced. An HVR1 level only match between two people could be from thousands to tens of thousands of years ago.

According to Dr. Doron Behar’s paper, A ‘‘Copernican’’ Reassessment of the Human Mitochondrial DNA Tree from its Root, dating haplogroup formation, haplogroup C was formed about 24,000 years ago, give or take 5,000 years in either direction, and haplogroup X was formed about 32,000 years ago, give or take 12,000 years in either direction. There are individuals living in Europe and Asia, as well as the Americans who fall into various subgroups of haplogroup C and X, which are impossible to differentiate without testing beyond the HVR1 region. A match at the HVR1 level which only indicates C or X, without subgroups, could be from a very ancient common ancestor, back in Asia and does not necessarily indicate Native American heritage without additional testing. What this means is that someone whose ancestors have never lived outside of China, for example, would at the basic haplogroup level, C, match to the Beothuk remains because they shared a common ancestor 24,000 years ago.

Furthermore, many people are tribally enrolled whose mitochondrial or Y DNA would not be historically Native, because their tribal membership is not based on that ancestral line. Therefore, tribal membership alone is not predictive of a Native American Y or mitochondrial haplogroup. Matching someone who is tribally enrolled does not mean that your DNA is from that tribe, because their DNA from that line may not be historically Native either.

Tribes historically adopted non-Native people into the tribe, so finding a non-Native, meaning a European or African haplogroup in a tribal member is not unusual, even if the tribal member’s enrollment is based on that particular genealogical line. European or African DNA does not delegitimize their Native heritage or status, but finding a European or African haplogroup in a tribal member also does NOT mean that those haplogroups were historically Native, meaning pre-Columbian contact.

Worse yet, one company is taking this scenario a step further and is informing their clients that carry non-Native haplogroups that they have Native heritage because a group of their clients who “self-identified” as “Native,” meaning they believe their ancestor is Native, carry that haplogroup. The American myth of the “Indian Princess” is legendary and seldom do those stories pan out as accurate with DNA testing and traditional genealogical research. Basing one client’s identification as Native on another client’s family myth without corroboration is a mind-boggling stretch of logic. Most consumers who receive these reports never go any further, because they have achieved what they sought; “confirmation” of their Native heritage through DNA.

A match, even in the best of circumstances where the match does fall into the proven Native haplogroups does not automatically equal to tribal affiliation, and any company who suggests or says it does is substantially misleading their customers.

From the Accu-Metric site, the company that identified the woman as Beothuk:

Native American linkage is based on a sample comparison to a proven member of the group, which identifies specific tribal linkage.

New for 2016: We can also determine if you belong to the 56 Native tribes from Mexico.

The DNA results can be used in enrollment, disenrollment, claiming social benefits, or simply for a peace of mind. We understand the impact that this testing service has on the First Nation and Native American community and we try to use our expertise for the community’s overall interests.

From Dr. Steven Carr, a geneticist at Memorial University in St. John’s (Canada) who has studied the Beothuk:

We do not have enough of a database to identify somebody as being Beothuk, so if somebody is told [that] by a company, I think we call that being lied to.

I would certainly agree with Dr. Carr’s statement.

According to the 2007 Beothuk paper, the Beothuk mitochondrial DNA fell into two of the 5 typical haplogroups for Native American mitochondrial DNA, C and X. However, only portions or subgroups of those 5 haplogroups are Native, and all Native people fall someplace in those 5 haplogroup subgroups, as documented here.

The Beothuk remains would match, at the basic haplogroup level, every other Native person in haplogroup C or X across all of North and South America. In fact, the Beothuk remains match every other person world-wide at the basic haplogroup level that fall into haplogroups C or X.  It would take testing of the Beothuk remains at the full sequence level, which was not possible due to degradation of the remains, to be more specific.  So telling a woman that she matches the Beothuk was irresponsible at best, because those Beothuk remains match every other person in haplogroup C or X, Native or not.  Certainly, a DNA testing company knows this.

Accu-Metrics isn’t the only company stretching or twisting the truth for their own benefit, exploiting their clients. Dr Jennifer Raff, a geneticist who studies Native American DNA, discusses debunking what she terms pseudogenetics, when genetic information is twisted or otherwise misused to delude the unsuspecting. You can view her video here. About minute 48 or 49, she references another unethical company in the Native American DNA testing space.

Unfortunately, unethical companies are trying to exploit and take advantage of the Native people, of our ancestors, and ultimate of us, the consumers in our quest to find those ancestors.

Reputable DNA Testing

If you want to test for your Native heritage, be sure you understand what various tests can and cannot legitimately tell you, which tests are right for you based on your gender and known genealogy, and stay with a reputable testing company. I recommend Family Tree DNA for several reasons.

  • Family Tree DNA is the founding company in genetic genealogy
  • They have been in business 16 years
  • They are reputable
  • They are the only company to offer all three types of DNA tests
  • They offer matching between their clients whose DNA matches each other, giving you the opportunity to work together to identify your common link
  • They sponsor various free projects for customers to join to collaborate with other researchers with common interests

When evaluating tests from any other companies, if it sounds too good to be true, and no other company can seem to provide that same level of specificity, it probably is too good to be true. No company can identify your tribe through DNA testing. Don’t be a victim.

These three articles explain about DNA testing, and specifically Native DNA testing, and what can and cannot be accomplished.

4 Kinds of DNA for Genetic Genealogy

Proving Native American Ancestry Using DNA

Finding Your American Indian Tribe Using DNA

For other articles about Native American DNA testing, this blog is fully key-word searchable by utilizing the search box in the upper right hand corner.

John Iron Moccasin, The Story of a Sioux Man

Occasionally, the project administrators of the American Indian project are presented with a rare opportunity to test an individual who is either full-blooded Native or nearly so. Recently, a Native Sioux man, John Iron Moccasin, born Earl White Weasel, stepped forward.

In order to facilitate testing, project members and others contributed funds with the agreement that we could publish John’s results and story. Now that the original tests are complete and we are publishing his results, we would like to upgrade John’s Y markers to 111 (from 37) and add the Big Y test – so if you’re inclined to contribute to the American Indian Project for this advanced testing – you can do so by clicking here.

But first, perhaps you’d like to hear John’s story. The results of the research into John’s history, both genealogically and genetically are fascinating. I hope you’ll get a cup of coffee or tea and enjoy this journey. Come along – we’re going on an adventure to South Dakota and we’ll be visiting the Sioux people!

In the Beginning…

A few months ago, John Iron Moccasin was talking to his friend and told her that he would like to share not only his oral history, but his genealogy and genetic history, with his daughter. He didn’t know how to go about doing either, but that friend, Pam, did, and she turned to me.

John was born as Earl White Weasel on Eagle Butte Reservation in South Dakota. He then lived at Cherry Creek Reservation in South Dakota. After adoption, he relocated to Pine Ridge Reservation, Kyle Reservation and then Oglala Reservation.

Unlike many adoptees, John always knew the identity of his birth parents and has given permission to use both his birth and adopted surnames. He takes pride in both, as well as his heritage. However, since John’s genetic genealogy is connected only with his biological parents, that’s where this article will focus.

Both of John’s biological parents belonged to the Cheyenne Sioux tribe. His birth father was Timothy Urban White Weasel and his birth mother was Martha Hale.

John is tribally enrolled with the Cheyenne Sioux based on his birth parents. John’s card shows his “degree of blood” to be at least 15/16ths.

Let’s take a look at tracking both John’s maternal and paternal ancestry. Many people ask how to work with Native records, and this article will follow my step-by-journey with both John’s traditional genealogy as well as his genetic genealogy, tracking each line back in time. But first, let’s look at the history of the Sioux people.

The Sioux

The Sioux are groups of Native American tribes and First Nations peoples in North America. The term can refer to any ethnic group within the Great Sioux Nation or to any of the nation’s many language dialects. The Sioux comprise three major divisions based on language divisions: the Dakota, Lakota, and Nakota.

The Santee Dakota reside in the extreme east of the Dakotas, Minnesota and northern Iowa. The Yankton and Yanktonai Dakota reside in the Minnesota River area. They are considered to be the middle Sioux, and have in the past been erroneously classified as Nakota. The actual Nakota are the Assiniboine and Stoney of Western Canada and Montana. The Lakota, also called Teton are the westernmost Sioux, known for their hunting and warrior culture.

The Treaty of Fort Laramie in 1868 established the Great Sioux Reservation, shown below, much of which has been whittled away today.

Today, the Sioux maintain many separate tribal governments scattered across several reservations, communities, and reserves in North Dakota, South Dakota, Nebraska, Minnesota, and Montana in the United States; and Manitoba and southern Saskatchewan in Canada.

sioux-map-today

By User:Nikater – Own work by Nikater, submitted to the public domain. Background map courtesy of Demis, http://www.demis.nl., Public Domain, https://commons.wikimedia.org/w/index.php?curid=2309029

The Dakota are first recorded to have resided at the source of the Mississippi River during the seventeenth century. The source of the Mississippi trickled out of Lake Itasca in present day South Clearwater, Minnesota. On the map below, you can see that location as well as Eagle Butte, to the west (larger white circle in South Dakota), some 300 or more miles as the crow flies, where John Iron Moccasin was born. The third location, Wilsall, Montana, on further west (red balloon), is where the remains of the 12,500 year old Anzick Child were found with Clovis tools.

im-lake-itasca

By 1700 some Sioux had migrated to present-day South Dakota. John’s Native ancestors were born in North Dakota, South Dakota, Montana, Nebraska and reportedly, Canada.

Late in the 17th century, the Dakota entered into an alliance with French merchants. The French were trying to gain advantage in the struggle for the North American fur trade against the English, who had recently established the Hudson’s Bay Company.

The first recorded encounter between the Sioux and the French occurred when Radisson and Groseilliers reached what is now Wisconsin during the winter of 1659-60. Later visiting French traders and missionaries included Claude-Jean Allouez, Daniel Greysolon Duluth, and Pierre-Charles Le Sueur who wintered with Dakota bands in early 1700. In 1736 a group of Sioux killed Jean Baptiste de La Vérendrye and twenty other men on an island in Lake of the Woods. However, trade with the French continued until after the French gave up North America in 1763.

For the most part, Sioux contact with Europeans was very limited until in the 1800s, and then, it turned deadly in a series of “wars” as the Sioux tried to protect their land and way of life. Europeans were equally as determined to eradicate the Indians, take their land and eliminate their way of life – and ultimately – they succeeded by containing the Sioux on reservations.

Records, other than oral history in the Sioux tongue, didn’t begin until Europeans began keeping them, so our earliest genealogical records of the Sioux only reach back into the 1800s. Thankfully, genetic records can reach back infinitely into time.

Let’s visit John Iron Moccasin’s ancestors, beginning with John’s paternal line.

The White Weasel Line

John’s father was Timothy Urban White Weasel, born August 1, 1939 to Oscar White Weasel and his wife, Esther (also called Estella) Ward. Timothy died March 28, 2004 in Eagle Butte, Dewey County, SD, the same location where he was born.

im-white-weasel

John’s grandfather, Oscar White Weasel is listed as a farmer in the 1930 census in Ziebach County, South Dakota, in Township 8, district 59 as a full blood Sioux male with a note “74-5,” speaking Sioux, as is his wife, Esther, age 24. They have been married 5 years and have two children, Margie age 4 & 9/12 and Beatrice, age 2 & 5/12th. Oscar is a veteran.

Please note that you can click to enlarge any graphic.

im-1930-white-weasel

This means John’s grandfather was born about 1898 and his grandmother about 1906. It should be noted that many traditional Native people have only a general idea of when they were born.

The US Department of Veterans Affairs Death File shows that Oscar Weasel was born on Feb. 22, 1898 and died on February 12, 1979. His military service was from March 28, 1917 to May 12, 1919.

The 1940 census from the same location shows Oscar J. White Weasel, age 42, wife Esther M., age 38, both Indian, both born in South Dakota, both educated through 7th grade, with 5 children including baby Urban J. White Weasel, age 7/12th. They live in Cherry Creek in Ziebach County, SD in the same place they lived in 1935.

im-1940-white-weasel

The Rapid City, SD obituary index shows that two obituaries for Oscar exist.

Weasel, Oscar J. 80 12 Feb 1979 Fort Meade, SD BHN 14 Feb 1979 p.31

16 Feb 1979 p.5

BHN means that Oscar is buried in the Black Hills National Cemetery. Find-A-Grave shows that he is buried in Section C, site 455 and that he was a PFC in WWI.

im-oscar-white-weasel-stone

im-black-hillsThe Social Security Claims Index shows that Oscar’s wife was Esther Ward and their child that filed the claim is Beatrice Louise Janis.

The 1927 Indian Census of the Cheyenne River Sioux Agency provides a little more information.

Joseph, also known as Oscar White Weasel is listed as born in 1898 and with two numbers instead of an English name. 322986 and 328110. I suspect these are the governmental identification numbers assigned to his parents when they were paid from the settlement fund – although one of those numbers could he his. His wife is listed as born in 1903 and as Mrs. Joseph White Weasel, nee Esther Ward, and she has one number listed in place of English name, 359087. Their daughter Margie is listed as born in 1925 and has no number listed by her name. There are no additional White Weasel individuals listed.

The 1925 Indian Census (below) shows us that he is listed as Joseph with Oscar penciled in above the name, with the number 322986 beside his name – which is evidently his number.

im-1925-census-white-weasel

The numbers probably related to the numbers assigned to Indians on the Dawes Rolls resulting from the Dawes Act of 1887 which allotted tribal lands in severalty to individual tribal members in exchange for Native Americans becoming US citizens and giving up some forms of tribal self-government.

In the South Dakota 1925 census, Joseph White Weasel is listed as married in 1924 and as Catholic. The South Dakota Marriages lists them as having married on October 18, 1924 in Cherry Creek.

im-white-weasel-marriage

Many of the Native people were “converted” to Catholicism by missionaries. The French were Catholic and the traders in this region and throughout the Great Lakes were French.

The 1900 federal census (below) lists Joseph White Weasel, born in 1898 as the son of Charley White Weasel born in April of 1866 in South Dakota. They are living on the Cheyenne River Indian Reservation, district 48 in Dewey, County, South Dakota. Joseph’s mother is “Follows” and she was born in July of 1869 in Montana, as were both children. They have been married 12 years, had 5 children, and 2 are living. Joseph’s older brother is Wakes (probably Makes) Believe his (probably he’s) Running. Charley is listed as “Indian Police” and Follows is listed as “Ration Indian.” They have not attended school, cannot read or write and do not speak English.

im-1900-census-white-weasel

The bottom of the census document includes an area called “special inquiries relating to Indians.”

im-1900-census-special-inquiries-white-weasel

This entire family is listed as Sioux, with no white blood. The mother and father of both Charley and Follows are listed as Sioux as well. They are not polygamous and they lived in a fixed, as opposed to moveable, structure. In other words, a “house” of some sort, not a teepee.

Polygamy was considered a grave sin by most Christian religions, and clearly someone still practicing the Native ways, which includes both polygamy and living in teepees, was highly encouraged to abandon those practices.

Note in the Indian census as late as 1902, some households are still listed with wife 1 and wife 2. It’s impossible to tell which child was born to which wife.

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Also note that the Native name and English name may have nothing to do with each other. They are not always literal translations. Please also note that Follows Him, above, is not the same person as Follows.

Christianity, and specifically Catholicism, along with “civility,” meant taking English names and living in established locations in structures. These behaviors were strongly encouraged and then forced upon the Native people with the Indian Citizenship Act of 1924 when their children were sent to “boarding schools” to learn the white ways, renamed, and it became illegal to practice the Native ways, including spiritual practices, powwows and speaking their own language. These restrictions lasted until the Native American Languages Act of 1990 which once again allowed Native people to speak their own language and the 1978 American Indian Religious Freedom Act allowing Native people once again to hold events such as powwows and practice their own belief system.  Unfortunately, the half century plus between 1924 and 1978/1990 successfully eroded and destroyed much of the Native cultural heritage.

Follows continues to be listed in the Indian census documents. 1895 is shown below.

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The 1897 Indian census (below) shows Follows and White Weasel with Makes Believe he is Running and a new child, aged 2. This child is not yet named, which makes sense in the Indian culture because children are not named until they “earn” a name of some sort. In some tribes, names are changed as new names are earned.

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The family is also shown in the Indian Census of 1899 (below) where Joseph has been named, in 1900, in 1902 when Lucy has been born, in 1903, in 1904, in 1906 when Lucy is no longer with them, and in 1907.

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The June 30, 1909 Indian Census shows Follows, age 40, but White Weasel is gone and she is shown with both sons, below.

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The 1910 federal census shows a Louise Weasel on the Cheyenne River Indian Reservation, with sons Peter age 17 and Oscar, age 11. I don’t know if this is the same family with white names, or this is a different family. I suspect that Follows has been “renamed” Louise for the federal census document.

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The 1910 Indian census shows Follows with both boys again as well as in 1911, 1913, 1914, 1915, 1917.  In 1918, Follows is shown with only Joseph.

I cannot find either Follows or Joseph (Oscar) White Weasel in the 1920 census, although he was clearly living because he married in 1924. It’s unclear when Follows died.

The Ward Line

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John’s grandmother, Esther Ward is listed as Esther White Weasel born in 1904 on the 1945 South Dakota Census, with both of her parents born in South Dakota.

On the 1910 federal census, Esther Ward is 6 years old living with her father, Alfred Ward, age 32, married 13 years, and his wife Nellie age 28. They have another daughter, Mary, age 12 and (apparently) a son, Alec Chasing Hawk, age 2. Alec’s father is listed as having been born in Montana and mother South Dakota, white everyone else and their parents are listed as born in South Dakota – so Alec is a bit of an enigma. They also live with a man I would presume to be Alfred Ward’s’s father, although he could be Nellie’s father, as he is listed only as “father” but generally that is the relationship to the head of the household. Jerome Chasing Hawk, age 78, so born in about 1832, widowed, Sioux, a Ration Indian. However, we later discover that Alfred Ward’s father is Clarence “Roan Bear” Ward and his mother is Estella DuPris, so the identity of Jerome Chasing Hawk is quite a mystery.

Ration Indian means that they are receiving rations from the Bureau of Indian affairs, often in exchange for land traded by the tribe.

Alfred raises stock and both Alfred and Nellie can read and write, but Jerome cannot.

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In the special inquiries section, Alfred Ward is listed as ¾ Indian and ¼ white, married once, not living in polygamy, received an allotment in 1908 and is living on his own land.

Nellie is listed as full Indian, received an allotment in 1909 and has been married once.

Jerome Chasing Hawk is listed as full, married twice, not living in polygamy, and received an allotment in 1903. He is not living on his own land.

The 1900 federal census shows Chasing Hawk, a widower, as the father-in-law of Dirt Kettle, whose wife is Woman Eagle. Chasing Hawk is 68 and was born in May of 1832 in South Dakota. His father was born in an unknown location and his mother was born in North Dakota. He is a Ration Indian and does not read, write or speak English. In the special inquiries section, Chasing Hawk is noted with other name as “Cetan, unknown” and that he is full Native.

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I did not solve the mystery of Chasing Hawk’s relationship to this family.

If Alfred Ward is indeed ¼ white, then John Iron Moccasin is 1/32nd white, assuming all other ancestors were full Native.

The 1900 federal census shows Alfred Ward, age 22, with wife Pretty Voice, age 16 and daughter Irelia Ward, age 1.

Pretty Voice appears to be Nellie’s Native name.

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In the special inquiries section, Alfred is listed with both parents being Sioux, but listed as half white. Pretty Voice is listed as Sioux, all Indian with no white. He can speak English, she cannot. Alfred is shown in the photo below.

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On the 1925 Indian Census Roll, Alfred and Pretty Voice are both shown. He has number 246235 or 246285 next to his name and she has 248261 beside her name. They have 3 children.

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On the 1931 Indian Census Roll, Joseph White Weasel is listed with his wife, Esther, with their roll numbers and the identification numbers of their allotment, annuity and identification numbers.

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On the 1895 Indian census, Pretty Voice is listed as the child of Hump and White Calf is listed as Hump’s wife, although we will see in a minute why that may not mean that White Calf is Pretty Voice’s mother.

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This is a very interesting development, because Hump and White Calf are also in John Iron Moccasin’s mother’s line, as are Clarence Ward and Estella DuPris.

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The 1886 Indian Census shows Hump, age 45, with wife Beautiful Hail, age 26, and daughter Pretty Voice age 3 and Her Voice, age 2. This strongly suggests that Pretty Voice’s mother was Beautiful Hail and not White Calf.

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The 1891 Indian Census labeled “Sioux of different bands” shows Hump, age 43, his wife designated only as “Mrs.” age 21, With Pretty Voice, age 9, Sun age 6 and Hope or Hoop age 2.

The 1892 Indian Census shows that Hump, age 42, married to White Calf, with daughter Pretty Voice, age 11, Sun age 8 and Hope age 2. Her Voice is not with the family, so presumably has died.

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Pretty Voice is reported on another tree maintained by YanktonSiouxTribe, who indicates they are a professional genealogist, to be the daughter of Chief Hump, friend and mentor to Crazy Horse. YanktonSiouxTribe reports that Pretty Voice married Alfred Ward, son of Roan Bear also known as Clarence Ward and Estella Dupris, the daughter of Fred Dupris and Good Elk Woman whose photo is shown below.

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Good Elk Woman

In the 1895 Indian Census, Alfred Ward is shown living with his parents, Clarence Ward and Estelle Ward, ages 44 and 40, respectively. They would have been born in 1851 and 1855. Clarence and Estelle’s youngest son, Willie, is also John’s ancestor through his mother’s line, having married Hope (Dora) Hump.

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It’s interesting to note in reviewing the Indian census records that in the mid-1890s, many Native people did not have an English name. Some had both, but far less than half in this tribe. However, by the 1920 federal census, they all had white names.

The 1900 census shows us that Clarence Ward was born in July of 1850 in Nebraska and his parents were both born in South Dakota. He is listed as Missionary R and his wife is listed as a Ration Indian. The “R” is noted beside a number of occupations, so I would presume he is a missionary and the R may indicate “ration Indian” as well. They have been married 21 years and she has had 5 children, 4 of whom are living.

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In the special inquiries section, Clarence is listed as Sioux, as are his parents. Estella and her parents are also listed as Sioux, but she is listed as one half Native.

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In 1920, Clarence Ward was living, age 67, no occupation, wife Stella, age 64. Both were born in South Dakota and are living on the Cheyenne River Indian Reservation in SD.

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Clarence is reported to have died in 1933.

Stella, or Estella DuPris, was born in August 1854 to Frederick DuPris and Good Elk Woman and died on July 6, 1927. Stella married Clarence Ward (shown below), who was born in 1851 in Nebraska.

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In the 1886 Indian Census, Clarence is shown as 35, Estelle as 31 and Alfred as 9.

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The 1900 Federal census shows Clarence as a Missionary, Estelle as born in South Dakota, her father born in France and her mother born in South Dakota.

DuPris Line

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Stella’s father, Frederick Dupris, was born in 1813 in Quebec City, Quebec and died in 1898. He had 10 children with Good Elk Woman between 1845 and 1870. He died on June 16, 1898 in South Dakota. Good Elk Woman, also known as Mary Ann DuPris, died on February 13, 1900.

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Photo of Fred DuPris and his wife, Good Elk Woman and Son, Xavier Dupris, courtesy, South Dakota Historical Society.

In case there is any question about whether Fred DuPris was 100% white, the 1900 census lists his son, Fred Dupris as Sioux, father white, mother Sioux and he being one half Native. This, of course, indicates that Fred Sr. was all white.

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In the Indian Census of 1894, Good Elk Woman is listed as age 68 and is living with her daughter.

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Good Elk Woman was the daughter of One Iron Horn born about 1805 in South Dakota and Red Dressing born about 1810. Good Elk Woman was originally married to hereditary chief, Henry Makes Room and had a son, Henry Makes Room Junior.

The following information was provided by Calvin Dupree.

“The First Dupree Into South Dakota”

Frederick Dupuis came from Longueil, Quebec to Kaskaskia, Illinois and from there to the Cheyenne River area. One Dupuis brother, Pierre (known as Peter), went on up into Montana where he married an Assiniboin Sioux woman.

A French-Canadian, Fred Dupuis arrived at Fort Pierre in 1838 and was in employee of the American Fur Company under Pierre Choteau, Jr. Letters from the winter of 1861 were written to Charles Primeau from Fred Dupuis by M. C. Rousseau at the mouth of Cherry Creek. The letters were concerned with reports of the Indian bands and the number of buffalo robes Fred was sending in and a list of the materials he needed for trading and maintaining his small outpost at the mouth of Cherry Creek. The trader (Fred) was concerned that the buffalo were becoming scarce and that the Indians and their horses were “poor”.

By 1860, we must assume that Fred was married and busy with the affairs of a husband and father. He married a Minniconjou, Good Elk Woman, who became Mary Ann Dupuis. She had one son, Henry Makes Room, from a previous marriage who was adopted by Fred. Mary was the daughter of One Iron Horn and Red Dressing. Some elders in the family remember that Mary was from Cherry Creek. Mary and Fred had nine children. They were: Peter; Maggie (Fisherman); Esther (Ward); Edward; David Xavier; Alma (Blue Eyes); Fred, Jr.; Josephine (Vollin); Vetal; and Marcella (Carlin). “Not one of whom could speak English, with the exception of Edward, who was a student at Hampton, Va.”

After being an independent trader for some time (and probably as the buffalo dwindled and the Indians were put on reservations) Fred became a stock grower. He built the family home in a beautiful wooded flat on the north side of the Cheyenne River, thirty-five miles west of where it emptied into the Missouri. The patriarchal home was described as being 20 feet by 60 feet, and built of cottonwood logs. As each son or daughter married, a new small log house (called a tipi by the family) was built. These homes had dirt floor and gumbo roofs and were placed in a row near the main house. In addition there were usually a dozen tipis nearby, pitched by the full blood relatives of Mary Dupuis. The living arrangement was truly communal; the women had a large vegetable garden; the men worked the stock; all the cooking and eating was done in one cabin. One of the women baked all the bread, another cooked the meat and vegetables, and another made coffee and served the food. Three times a day 52 people ate together, along with any strangers or friends who might happen along.

The Dupuis home was known as a place for sharing good times and good food in the true Indian way. This was the era of government ration dispensing and all 52 of the family members collected their share which was hauled home in wagons from Fort Bennett, even though Old Fred was reputed to be wealthy with “several thousand head of cattle and 500 horses, a small herd of domesticated buffalo and a large amount of other property.”

The marriage of Marcella Dupuis, Old Fred’s youngest daughter, to Douglas F. Carlin, a non-Indian, of Pierre must have been a noteworthy event since newspapers from Deadwood and Pierre covered the event. Mr. Carlin was noted as the issue clerk at Cheyenne Agency. The ceremony was performed at the Dupuis home on the Cheyenne River with many important persons from the city, including the Pierre City Council, and unknown numbers of Sioux present. Forty fat steers were to be roasted. All the wedding gifts were put on exhibition after the supper, the most impressive being five hundred head of cattle and fifty ponies from Old Fred, father of the bride, and a decorated buffalo robe from sisters of the bride. The Sioux dancing continued for three days with the only interruption being a pause for more eating every three hours.

The Dupuis family’s contribution to saving the buffalo.

In 1883 (or possibly earlier) Old Fred and some of his sons and possibly Basil Clement (Claymore) went on a hunt for some buffalo calves in order to start a herd. By this time the great “surrounds” of the past were over and I can imagine that the desire to preserve at least a few of these animals, so necessary and so sacred to the Indian people, was strong. The group headed northwest from the Cheyenne River and was gone for many months and in Montana, or near Slim Buttes (reports differ), they located a small herd. They finally secured five calves (one report says nine), which were loaded into wagons brought along for that purpose. The calves were taken back to Cheyenne River.

By 1888 from this small start the Dupuis had nine pure-blood buffaloes. By the time of Old Fred’s death in 1898 the herd had grown considerably, and was purchased by James (Scotty) Philip of Fort Pierre. By 1918 (the herd) had increased to approximately 500 head. The State of South Dakota purchased 46 of these buffalo and transferred them to the State Game Park in Fall River County. Hearsay has it that Scotty Philip sold buffalo to other states and parks also, spreading the original Dupuis stock back into many areas where the buffalo once roamed free by the millions.

Old Fred died in 1898 at about age 80. Then, as now, a death was the occasion for sharing through a Give-Away of all the deceased’s belongings. From Aunt Molly Dupris Annis Rivers, Old Fred’s grand-daughter, I have heard the colorful story of how some of the Dupuis wealth was distributed. It is said that according to Lakota custom, any one who happened by was entitled to a gift and this even included a group of Crow Indians, traditional enemies of the Sioux since anyone can remember. The Crows were invited to join the other guests as they filed by a horse whose saddle bags had been filled with silver dollars. Each person took a silver dollar until they were gone; the next person in line was given the saddle, and the last person received the horse. And in this way, and probably by several other methods, Old Fred’s money and property were shared with the people. None of his oft mentioned wealth was inherited by any of his family.

Records indicate that Good Elk Woman, Mary Dupuis, died in 1900 at the home of her daughter, Mrs. Tom (Alma) Blue Eyes. One can only wonder about her life after Old Fred died, just as one wonders about her years of living, first as a child at Cherry Creek, then as a young wife of Makes Room and finally as Mary Ann Dupuis, mother of nine half French and half Lakota children. No stories about Mary have come down to me. Her life during the early time of tragedy and defeat for the Indian people cannot have been an easy one.

Old Fred and Mary, and many of their descendants, are buried in the Dupuis Cemetery on the hill above the river flat where their family home once was. Nearby is the old ”Buffalo Church”.

Old Fred and Mary may be gone, but South Dakota will not forget them. Dupree Creek runs into Rudy Creek and then into the Cheyenne River near the old home site, and the (town) of Dupree is located about 40 miles north of Cherry Creek where Old Fred carried on his fur trading. Just west of the Dupuis cemetery and the old church, in a draw filled with wild plums and chokecherries, the Dupree Spring (called the Circle P Spring, or Garrett Spring today) still furnishes clear, sweet water.

Imagine the hundreds of trips made to this spring, winter and summer, to haul water for the Dupuis family living down the hill by the river in the 1800’s.

The name, though changed from Dupuis to Dupris and in some cases to Dupree, has been carried all over South Dakota and to probably every state in the U.S. by their hundreds of descendants.

Calvin Dupree is the son of Adelia Fielder and Jonas E. Dupris; son of Sarah Red Horse and Frank Dupris; son of Harriet Cadotte and Xavier (David) Dupuis; son of Mary Ann Good Elk Woman and Frederick Dupuis. Calvin Dupree is presently a member of the faculty of Education at the University of Lethbridge in Alberta, Canada.

According to Suzanne DuPree, a descendant, Fred DuPris (in later generations spelled DePree), and Good Elk Woman are buried in the DuPris Memorial Cemetery on the hill above the river flat where their family one was once location, near the old “Buffalo Church.”

FindAGrave lists Fred DuPris’s birth date as September 5, 1819 and his death as July 16, 1898. His wife, Mary Ann, born as Good Elk Woman, is shown as being born in 1824 and passing over on February 13, 1900. The maps below are from FindAGrave.

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The Sioux Chief, Hump’s Line

John descends from Chief Hump twice, apparently through two different wives; Beautiful Hail and White Calf. John Iron Moccasin’s family information indicates that Hump had 4 wives: Good Voice/Good Woman, Brings Her, Stands As A Woman and Bessie/White Calf Woman. The census provides information about Beautiful Hail and White Calf, but we have no further information about Humps’s other two wives.

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Hump, also known as Thomas Hump, lived until December 11, 1908 where he died in Cherry Creek, SD.

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Photo courtesy of the South Dakota State Historical Society

Born in Montana, Hump became a leader of the Cherry Creek Band of Minneconjou Sioux. In 1876 he fought in the Battle of the Rosebud against Gen. Crook, shown below in the wood engraving below depicting the Sioux charging Colonel Royall’s attachment on June 17th.

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Hump also fought on Calhoun Hill in the Battle of the Little Big Horn with Crazy Horse, Gall and others against Custer and the 7th Calvary on June 25th where he received a bullet wound in his leg, according to the National Park Service.

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The Lakota Museum and Cultural Center tells us the following about Hump.

Etokeah, a Minniconjou Lakota war chief, was a great leader. He is especially known for his skills during the 19th Century Lakota-US Government battles. His exact birth date and facts of parentage were not recorded. However, he first came into public notice in 1866. Then, he led the charge against Captain William Fetterman’s soldiers outside Fort Phil Kearney in Wyoming.

Hump did not sign the Treaty of Fort Laramie in 1866. Because of his action, he was deemed a hostile or “non-treaty” chief by the US Government. He was a comrade-in-arms of Crazy Horse, Red Cloud and other great Sioux chiefs of the period. In 1876, he led his warriors into battle against Generals George Crook and George Custer.

After the defeat of the Sioux in the 1880s, he briefly lived in Canada. He eventually returned to the United States but remained hostile to the whites. In company with most of the Sioux, his band was intrigued by the Ghost Dance religion, which culminated in the massacre at Wounded Knee Creek in 1890.

Although Hump seems never to have become a true believer, he did lead his people in the Ghost Dance raids until early December of 1890. The US Army was alarmed by the Ghost Dance, and they sent emissaries to all of the major chiefs.

Captain Ezra Ewers – an old friend – was sent to speak with Hump. Ewers convinced Hump of the futility in armed resistance. At this point, Hump separated his band from the Dancers and led them to the Pine Ridge Agency.

As Hump was breaking camp, refugees from Sitting Bull’s group arrived and related how their leader had been killed during an arrest attempt. Sitting Bull’s people were eager to find allies as they sought revenge. Hump refused to help, and the refugees set out to join Big Foot near Wounded Knee Creek.

After the infamous massacre and subsequent events in 1890, Hump and several other Sioux chiefs went to Washington, D.C. They pleaded for fair treatment of their people.

Some of their requests were honored; however, the chiefs failed to gain concessions in other important areas. Reservation confinement continued, effectively ending the old way of life.

Hump died at Cherry Creek, South Dakota on the Cheyenne River Sioux Reservation in December 1908 at the age of 70. He is buried in the Episcopal Cemetery near there.

According to records provided by John Iron Moccasin’s family, Hump’s father was Iron Bull “TaTankaMaza”, and his mother was Ziti “Yellow Lodge”. Hump was born about 1848 when his father was 28 and his mother was 21.

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This photo was taken ca. 1879 by photographer by L.A. Huffman. The notation is that the photo is of Hump and his favorite wives. One of these women could well have been Beautiful Hail given that she appears to have had children in both 1882 and 1883 with Hump. He does look to be significantly older than the women.

Hump is shown with other Sioux leaders in this 1891 photograph.

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1891 Sioux Delegation LA-NA-DA-Kota

Front Row Seated; L to R: High Hawk, Fire Lightning, Little Wound, Two Strike, Young Man Afraid of His Horses, Spotted Elk (Oglala), Big Road; (2nd row standing) F.D. Lewis, He Dog, Spotted Horse, American Horse, Maj Gen Sword, Louis Shangreaux, Bat Pourier; (3rd row, standing) Dave Zephier, Hump, High Pipe, Fast Thunder, Rev. Charles Cook, and P.T. Johnson. Denver Public Library

In the 1900 federal census of the Cheyenne River Indian Reservation, district 48 in Sterling County, SD, the last census in which Hump was alive, he is shown on the census as having been married 20 years, born in April 1850 in Montana, with both of his parents born in the same place. He is a Ration Indian and he does not read, write or speak English. In the special inquiries section, he is listed as Sioux, his father as Sioux Cheyenne and his mother as Sioux. He is listed as entirely Native and in this census, is not listed as polygamous.

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His wife is listed on the next page as White Calf to whom he has been married for 20 years, so dating back to 1880. Of course, as suggested by the picture taken circa 1879 and the 1886 census in which Hump is married to 26 year old Beautiful Hail, White Calf was not his only wife. Given that Pretty Voice appeared in the census in 1876 with Beautiful Hail as a young child, I would presume that Beautiful Hail is Pretty Voice’s mother.

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Dora, who was born in 1891, is not shown living with Hump. I cannot find her elsewhere on the census. However, remember that Native people changed their names. Hope is listed as being born in July of 1889 in Montana.

In the 1917 Indian Census, Hope Hump is also listed as Dora, age 26, married to Willie Ward who was born in 1889. This shows us that Dora is Hope or Hoop Hump on the earlier census records.

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According to the 1900 census, Hope was born in July of 1889 in Montana, as were both of her parents. She does not read, write or speak English. She is 100% Sioux.

The following information was provided by http://files.usgwarchives.org/sd/ziebach/history/chap16-2.txt

Born in Montana in 1848 or 1850, Hump became a leader of the Cherry Creek band of Minneconjou Sioux.   In 1876 he fought in the Battle of the Rose bud against General George Crook and in the Battle of the Little Big Horn.

He later joined Sitting Bull’s band and other exiles in Canada.  Being considered American Indians, the exiles received no rations from the Canadian government. By 1881 the buffalo and other game were disappearing and the exiles returned to Fort Buford where they surrendered. They were taken to Fort Yates by steamboat. Later the Minneconjou under Hump and Fool Heart and the Sans Arc, led by Spotted Eagle and Circle Bear, were taken down the Missouri River to the Cheyenne River Agency, near their traditional camping grounds along the Cherry Creek and Cheyenne River.  They arrived at the Cheyenne River by May of 1882 and many of the Minneconjou settled near Cherry Creek, 50 miles west of the agency.

Hump and Big Foot became the most influential men on the Cheyenne River.  The Cherry Creek/Hump Band greatly opposed the land agreements of 1888 and 1889.  In 1890, the Ghost Dance found its greatest following in the Cherry Creek camps.

After Sitting Bull was killed on the Grand River, many of his followers fled south and camped a few miles above the junction of the Cherry Creek and Cheyenne River.  When the army at Fort Bennett moved to suppress the Ghost Dancing, Hump used his influence against the Ghost Dance. In the dead of winter he rode with two men from the garrison and two other scouts, 40 miles to persuade the Sitting Bull camp to surrender and move to Fort Bennett.  Those who did not surrender joined Spotted Elk, also known as Big Foot. When his band later fled toward Pine Ridge, they were met by the Army at Wounded Knee.

Hump was given 500 heifers for his service to the United States Government. These he turned loose, to share with his people. The heifers wandered near Leslie and many died of pinkeye.

Hump continued to work for his tribe until his death in 1908. He is buried in Cherry Creek.

HUMP

Told by John Hump

Hump (Thomas) was born in 1850 to Mashes His Nails/Iron Bull and Ziti/Mrs. Iron Bull (1827-1917) in Montana.

Hump’s brother, Little Crow, had been born in 1844. Hump’s sister, White Cow, married Fish (d. 1919) and had a son, James Fish (b. 1889) and a daughter. They lived on Rosebud.

Hump grew up in Montana. He had three or four wives, some of whom lived in Montana and were Crow.

While the Indians still roved in bands, he started to gather them together, to settle down and become ‘civilized’. Hump came down the Missouri River when the Army brought them to the Cheyenne River on boats. Their stock were driven over land.  Bertha Lyman Hump’s mother’s family came from Montana with Hump’s band.

Hump even joined the Army to work toward settling down. He was a scout from December of 1890 until June of 1891. He was discharged at Fort Bennett.

There were three Hump Flats. One east of Bridger, one by Iron Lightning and one across from Cherry Creek. All are so named because he lived on them. On the way to Montana for a visit, Hump camped with Iron Lightning on the Moreau River. At that time they chose their allotments. Iron Lightning community was later named for Iron Lightning after he moved there.

Hump had several wives. His son, by Good Voice/Good Woman, was Samuel Helper/ Stand by of Oglala, born in 1876.

Hump’s wife, White Calf/Bessie (d. 1915) was the mother of Pretty Voice/Nellie (b. 1882: Mrs. Alfred Ward); Important Woman/Sarah (b.1884: Mrs. Silas Yellow Owl); Spotted Bear who died in infancy; Dora (b.1891: Mrs. William Ward); Didn’t Drop/Nelson Hump, born in 1898 (no issue); William Miles Hump, born in 1900 and died in 1917 at Dupree, (no issue); and John Hump, born in 1904.

JOHN HUMP

John Hump was born at Cherry Creek, four years before his father’s death in 1908. Hump is buried at the Episcopal Cemetery in Cherry Creek.  John went to Carson Day School, Pierre Indian School and Rapid City Indian School.  In 1935 or 1936, he married Bertha Lyman, daughter of Ed Lyman. John transferred his heir ship lands from the Moreau River to Red Scaffold.

John and Bertha lived on the flat south of the (Cherry) creek, on her folks’ allotments. In 1954/1957 they moved north to their present home.  John went into the cattle business on the Rehab program. John and

Bertha’s sons, Duane and Darrell, now run the ranch.

Darrell is married to Alvina Runs After and Duane is married to Doris Halfred.

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The 1910 census taken at Cherry Creek station shows us that White Calf’s mother was Roan Hair, age 72, so born about 1838. She shows the birth of only one child.

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The special inquiries section tells us that she is Teton Sioux, full Native, married once, not polygamous, lived in an aboriginal dwelling and received her allotment in 1903.

Roan Hair is shown in the Indian census of the Cheyenne River Sioux in 1896 as the wife of Ragged, both age 56.

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Four years later, in 1901, they are shown again.

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Hump died on December 10, 1908 and is buried in the Episcopal Cemetery in Cherry Creek, SD.

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Hump’s Memorial at FindAGrave adds some additional information not found elsewhere.

Native American Chief. Sioux name “Etokeah.” Although very little is known about Hump’s early life, he eventually rose to become a Chief among the Miniconjou Sioux and was an active participant in the Red Cloud war. With Crazy Horse at the Rosebud Battle against George Crook, Hump led his Miniconjou Sioux, helping stop the column in their trek to meet Custer prior to the Little Big Horn. At the Little Big Horn, when the alarm was sounded, Hump jumped onto an unknown mount, and it which threw him to the ground. Hump rushed, mounted another horse and charged toward the soldiers. His horse was shot from under him and a bullet entered above the horse’s knee and went further into Hump’s hip. Hump was strained there due to the wound and did not participate in the main battle. Later, Hump went to Canada, and his band returned to the United States, the last of all the bands to return. On the reservation when other tribes had adopted white dress and housing, Hump’s band settled at Cherry Creek in South Dakota and maintained the old ways using lodges and traditional clothing. On the reservation when the authority of other chiefs wained, Hump continued to assert leadership over his band. Some said that Hump was feared by the whites even more than Sitting Bull. When the Ghost Dance religion surfaced among the Sioux, the military did not dare arrest Hump. Instead, they reassigned Captain Ezra Ewers, a trusted friend of the chief, to Fort Bennet in South Dakota. Ewers rode the 60 miles to Hump’s camp at Cherry Creek. Impressed with Ewer’s courage, Hump listened to his message and avoided the Ghost Dance religion. After the Wounded Knee Massacre, Hump along with other prominent Sioux went to Washington, DC pleading for a peaceful end to the tragedy. Interestingly enough, it was also Hump who taught the basic lessons of warfare to his better-known student, Crazy Horse. His grave is located on the west edge of the town of Cherry Creek.

This photo of Cherry Creek, probably in the early 1900s, shows both traditional teepees and more stationary buildings. This lends understanding to the special inquiries section of the census, and shows us what “fixed” dwellings look like as compared to “moveable.”

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The Hale Line

John’s mother was the daughter of Isabelle Ward and Robert Hale.

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South Dakota Marriage records show that Robert Clifford Hale, age 23, married Isabel Ward on May 3, 1946. Both lived in Cherry Creek, SD.

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Robert died on August 1, 2008. His photo and obituary are shown below.

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Death: Aug. 1, 2008 Sturgis Meade County South Dakota, USA
Robert “Bob” Clifford Hale, who lived in Cherry Creek, had the Lakota name Min A’ Kyan, which translates to Flies Over the Sea. While he may not have flown over the sea, he did ride the sea as a sailor in the U.S. Navy during World War II. Bob, at age 85, died Friday, Aug. 1, 2008, at the Fort Meade Veterans Hospital near Sturgis. He is survived by Larry (Delia) Hale, Theresa Hale, Herbert Hale and Cleo Hale, all of Cherry Creek, Martha (Erick) Hernandez of Chicago, Ill., Richard Hale of Rapid City, Connie (James) Bear Stops of Red Scaffold and Lavinia Hale-Eagle Chasing of Eagle Butte; grandchildren, Maude Hale, Denise and Richard Crow Ghost, Dawn Kills Crow, Angelic and Willard Demery of Cherry Creek, Amber and Alton Blacktail Deer Sr. of Manderson, Timothy Jr., Earl and Mary Iron Moccasin of Rosebud, Teno, Taun and Krista Bear Stops of Red Scaffold, Rhiana, Richard Jr. and Joshua Hale of Cherry Creek, Angel Prendergast and Aberham White Weasel of Rapid City, Maxine Flying By, Marsha Eagle Chasing of Eagle Butte, Sarah, Elizabeth, Mark and Posey Garter of Albuquerque, N.M., and Clinton and Kyle Harrison of Takini. Also surviving are his great great grandchildren, Morgan and Jasmine Hale, Eric Jarvis and Dewey Kills Crow, Kyra, Danieal and Alyssa Hayes, Adrienne and Royce Jr. Marrow Bone, Eric, Jarvis, Dewey, Drake and Autumn Kills Crow, Shantay Crow Ghost, Alton Blacktail Deer Jr., La’tia, Tyree and Lashae Bear Stops, D’Nica Ducheneaux, Tretyn Red Elk, Sage Bowker, Sarah Patryas, Jordan and Sierra Iron Moccasin, and Kleigh, Dawnelle and Deaconn Garter. Robert was preceded in death by his parents, Joseph and Ellen Hale; sisters, Claira Hale-Fritz, Myrtle Hale-Little Shield, Don’ta Black Tail Bear, Drazen Black Tail Bear, Mary Isabbella Kills Crow, Clifford Merle Hale; brothers, Martin and Wilson Hale; one daughter, Charmaine Hale Harrison; and his paternal grandparents. Funeral services for Robert were Saturday, Aug. 9, at the new Community Building in Cherry Creek. Ted Knife, Erick Hernandez and Elmer Zimmerman officiated. Hernandez read Matthew 7:7. Special music was provided by Buzzy Yellow Hawk, Daryl Whipple, the Tiospaye Singers, Michelle White Wolf and the Mennonite Singers. Harvey Eagle Horse played the Honor Song. Casketbearers were Bob’s grandsons, Joshua Hale, Taun Bear Stops, Timothy White Weasel Hr., Clinton Harrison, Posey Garter, Maris Reindall, Richard Hale Jr., Teno Bear Stops, Eric V. Kills Crow, Kyle Harrison, Mark Garter and Danny Hayes Sr. Honorary bears included all military veterans and all Bob’s other friends and relatives. Burial was at the UCC Cemetery in Cherry Creek under the direction of Oster Funeral home of Mobridge. Mobridge Tribune Wednesday, August 13, 2008

The US Department of Veteran’s Affairs BIRLS Death File lists Robert Hale’s birth and death dates and his service branch as Navy from July 24, 1942 to November 27, 1942.

The Social Security death index shows that Robert was born on Sept. 7, 1922.

I cannot find this family in 1920, 1930 or 1940 in the census, nor in the Indian census. It’s possible that the parents and siblings names are incorrect or nicknames.

Robert’s parents were given as Joseph Hale and Ellen in his obituary. John’s mother reports that Joseph Hale’s name was Joseph “Blows on Himself” and that this is the end of that line because they migrated from Canada on “the big trail.” I found nothing about this family at Ancestry or utilizing Google. It’s possible that the family was not living as a nuclear family as a recognizable unit.

The 1940 census shows a Joseph Hale, age 48, widowed, an Indian, as an inmate in the Davison County, South Dakota Jail, but we don’t know if this is the same Joseph Hale.  However, this is the only Joseph Hale in South Dakota, or for that matter, in that part of the country.

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This Joseph was widowed, an Indian and born on an Indian Reservation, so it may well be the correct Joseph. It would be interesting to see if any court records still exist relative to this case.

I found scanty information on the following individuals from the obituary listing them as siblings of Robert Clifford Hale.

  • Claira Hale – married Elmer Fritz on February 27, 1962 , born about 1926.
  • Mytrle Hale – Myrtle Faye Hale married Theophil Little Shield and died in SD at age 65.
  • Don’ta Black Tail Bear – nothing
  • Drazen Black Tail Bear – nothing
  • Mary Isabella Hale Kills Crow – nothing
  • Clifford Merle Hale – nothing
  • Martin Hale – if the same Martin, died in 1935 of appendicitis, age 20.
  • Wilson Hale born about 1921 married Eunice Eagle Horse. He died in 1950 in Ziebach County. In the 1940 census he is living with the Straight Head family which would make sense if his mother was deceased and his father was in jail.

The Second Ward Line

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John Iron Moccasin’s grandmother on his mother’s side was Isabella Ward, born in 1925 or 1927.

The 1930 Federal census shows Isabella Ward, age 5, living with her parents in Ziebach County, SD. Her mother, Dora is listed as a full blood and her father, William, a mixed blood, all born in South Dakota and Sioux.

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Her father is listed as a farmer.

We’ve already met Dora (Hope) Hump, daughter of Chief Hump and probably White Calf and William Ward, son of Clarence “Roan Bear” Ward and Estella Dupris.

DNA Results

Now for the most exciting part – the DNA results. Do John’s DNA results bear out his genealogy?

John’s tribal card says that he is at least 15/16th Native. That is accurate, given that he is 1/16th French on both his mother and father’s sides, from the same ancestor.

In percentages, for autosomal DNA, that translates into 6.25% white and 93.75% Native.

When I’m working with descendants of tribes located east of the Mississippi, I understand that they are very likely heavily admixed with (primarily) European males, and significantly so prior to 1800 and in most cases, prior to 1700. However, the Sioux are somewhat different. Except for occasional traders and missionaries, they essentially escaped the widespread influence of Europeans until the 1800s. With few exceptions, I would not expect to find earlier mixing with Europeans, meaning English, French or Spanish, or Africans.

Because of the history of the Sioux tribe, the sheer number of Sioux across a wide geography, and the lack of early European admixture, John’s DNA represents an opportunity to obtain a genetic view of a people not significantly admixed.

Endogamy

We know from John’s family tree that he shares at least 3 ancestors and possibly 4 on both his mother’s and father’s side of the family. Those ancestors are 4 generations up the tree from John.

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In most cases, one’s great-great-grandparents would each contribute, on average, 6.25% of your DNA. In John’s case, he received a double dose of the DNA of each of those ancestors. If John received the exact same DNA from those ancestors, from both sides, he would still only have 6.25 % of their DNA. This is very unlikely, because normally siblings share part of their parent’s DNA, but not all of it. Conversely, it would be very unlikely for John to inherit none of the same DNA from that ancestor from both lines. Therefore, it’s most likely that instead of 6.25% of the DNA from that each ancestor who is found twice at 4 generations, he would carry about 9.38% of their DNA, or about half a generation closer than one would expect.

And that goes for all 3 common ancestors. We’re not sure which of Hump’s wives gave birth to which children, so this could also apply to Hump’s wife, a 4th ancestor.

Furthermore, these individuals in the tribes are likely already very heavily inter-married and related to each other, long before any records. There were only a limited number of people to select as mates, and all of those people also descended from the same ancestors, who were part of a very small foundation population that migrated from Asia some 10,000 to 25,000 years ago, depending on which model you subscribe to.

Therefore, endogamy and pedigree collapse where one shares common known ancestors would be a phenomenon that has occurred since the time of Anzick Child, and before.

John’s Tests

We tested John’s DNA at Family Tree DNA where his Y, mitochondrial and autosomal DNA was tested. John’s Y DNA shows us the deep ancestry of the White Weasel line. The mitochondrial shows us the deep ancestry of Dora (Hope or Hoop) Hump, daughter of Hump, presumably through wife, White Calf.

John’s autosomal DNA shows us an overall ethnicity view, plus matches to autosomal cousins. Let’s see what we have.

Autosomal Results

John’s myOrigins results show that he is roughly 17% European and the rest a combination of Native and Asian that together represents 84%.

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One of the aspects that I find most interesting is that the portion of Europe that shows a genetic link is Finland, not France where 6.25% of John’s paper trail ancestry is from.

Finland is particularly interesting in light of the result of the Clovis Anzick Child burial found in Montana that dates from about 12,500 years ago. We have the Anzick Child’s results in the Family Tree DNA data base, compliments of both Felix Immanuel and Family Tree DNA.

The Anzick child’s myOrigins results are shown below.

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The Anzick Child’s DNA ethnic results are very similar to John’s. Anzick Child matches the reference population for Finland at 11%, where John matches at 17%.

Furthermore, John Iron Moccasin is one of 110 people in the data base today that actually match the Anzick Child’s DNA at contemporary levels.

The match threshold at Family Tree DNA today is:

  • No minimum number of shared cM required, but if the cM total is less than 20, then at least one segment must be 9cM or larger.
  • If the longest block of shared DNA is greater than 9cM, the match will show regardless of total shared cM or the number of matching segments.

Lowering the match threshold to 3cM, we can see several small segments that match between John and the Anzick Child.

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I downloaded their common matching segments.

Chromosome Start Location End Location centiMorgans (cM) # of Matching SNPs
1 4282649 5290332 2.56 500
2 98863262 101324606 1.69 600
2 112439588 114460466 1.71 500
2 169362301 170609544 2.27 500
3 8964806 10632877 3.03 600
3 14230971 16121247 2.83 600
3 46655067 53174054 1.28 1000
4 12866760 14721835 1.85 500
5 78642903 80323930 1.64 500
5 158757557 162829228 3.82 1000
6 34609507 36812814 2.88 600
6 127839067 130105402 2 500
7 76597648 78055762 2.84 500
7 99319352 101758792 2.05 600
8 10455449 12975017 2.68 700
8 30301880 34206702 3.45 799
9 26018352 27374204 2.37 500
9 104470303 106854637 3.76 777
10 71258510 72644677 1.46 600
10 102514460 106018240 2.65 800
10 110936823 113553555 3.83 700
11 32265994 34530393 3.35 700
11 91619854 94670011 3.71 800
11 102068510 103853340 1.76 500
12 27332778 29165805 1.66 500
12 96875639 99784589 2.74 700
13 55048728 58723000 1.66 600
13 78707414 80906921 1.34 500
14 22564888 24752111 3.59 800
14 68418807 70225737 1.65 500
14 76767325 78038237 1.71 500
16 12528330 14375990 5.49 659
18 33126219 35069488 1.37 500
19 8284870 13355259 7.87 1278
20 45913972 47494552 3.17 500

Their largest matching segments are on chromosome 19 for 7.87 cM and on 16 for 5.49 cM.

The genetic connection between the Anzick Child and John Iron Moccasin is evident. John’s tribe is descended from the same people as the Anzick Child who was buried in present day Montana. John’s ancestors, Hump, Roan Hair and Follows were all born in Montana, and the Sioux homelands stretched across this entire region.

This begs the question of whether John is simply lucky to have inherited these segments, or if they are found widely in the Native, particularly Sioux, population as a whole.

To help answer this question, I looked at John’s closest 4 matches along with the Anzick Child in the chromosome browser, compared to John’s DNA.

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At 5cM there is no overlap with John’s closest matches and the Anzick Child, whose DNA is shown in green, above. However, dropping the threshold to 3, below, shows overlap with Thomas’s closest match on chromosome 19 at 4.98 cM and other chromosomes in smaller amounts. This would suggest that perhaps the DNA that is the same as the Anzick Child’s does not repose in the entire tribal population.

im-match-compare-3cm

Let’s take a look another way.

John and the Anzick Child at GedMatch

At GedMatch, John matches the Anzick Child on slightly different segments than at Family Tree DNA. It’s not unusual for different vendors to produce slightly different results. In this case, the match on chromosome 16 is absent altogether, and there are larger segment matches on chromosomes 8 and 14 using a 5cM and 500 SNP threshold.  Chromosome 22 shows a match not present at Family Tree DNA.

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I was curious to see how many people matched John on his segments shared with the Anzick Child.

John matches a total of 2119 people at GedMatch at 5cM and 500 SNPs.

John’s results for his two largest segments, chromosome 16 (at FTDNA) and 19 were different. Chromosome 16, the smaller match, was generally unremarkable, but his chromosome 19 was a different story, carrying many names and surnames that I recognize.

Let’s take a look at the triangulation tool and see what we find there. We are looking for anyone who triangulates with both John and Anzick Child. This tool reports every triangulated match in excess of 5cM.

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Using the triangulation tool, no one triangulates, meaning matches both John and the Anzick child, on either chromosome 16 or 19. This suggests that all of John’s matches showing are on the “other” chromosome and that this chromosome segment is fairly rare.

If one of John’s parents were to test, we could identify which of John’s parents was matching Anzick, so we would know which side of John’s family these individuals are matching on these segments, assuming these matches are not identical by chance.

Out of curiosity, I triangulated Anzick Child’s kit to see if there were any triangulated groups. There were, but none that included John.

At GedMatch, let’s use the “Are Your Parents Related?” utility. We know that John’s parents are related, but are any of the segments that came from both parents the same segment that is found in John’s Anzick match? The match threshold at GedMatch for this tool is 7cM and 700 SNPs, so the only segment that would qualify would be this segment on chromosome 19, shown above in green.

19 8284870 13355259 7.87 1278

The “Are Your Parents Related?” tool at GedMatch shows the following results.

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According to GedMatch, this segment of chromosome 19 was not contributed by both of John’s parents, so this portion of the Anzick DNA is not found universally in the entire Native population in that region.

One last look at John’s DNA by comparing to the Ancient group contributed at GedMatch shows no segments 4cM or above that match with any ancient specimen other than the Clovis (Anzick) Child, including no match to the Paleo Eskimo in Greenland from 4,000 years ago and no match to Kennewick Man. The tiny orange bars represent matching segments at 400 SNPs and 4cM.

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John’s Mitochondrial DNA

John’s mitochondrial DNA comes directly from his matrilineal line, meaning from his mother, her mother, her mother, on up the tree until you run out of direct line mothers.

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In this case, that person winds up being Hump’s wife. We think that person is probably  White Calf, but it could be one of Hump’s other wives. We just don’t know for sure given that Hump was polygamous.

Mitochondrial DNA is passed intact in each generation, doesn’t get combined with the father’s DNA so it’s a direct line back in time.

Johns’s mitochondrial haplogroup is clearly Native, C4c1.

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Haplogroup C4c1 was originally reported in the Suswap by Ripan Malhi; in the Chippewa Creek and in Jasper House, Alberta Canada, in 2015 by Roberta Estes from the American Indian project.

At the HVR1 level, John has 62 exact matches, but he has no matches at the HVR2 or full sequence levels. This means that of the people who have tested at that level, he has more than 4 differences at the full sequence level. Translated, this means they don’t share common ancestors in hundreds to thousands of years.

Only 8 of John’s HVR1 matches have tested at the full sequence level, unfortunately.

Of those, the earliest ancestors are Spanish, indicating that they are probably from either the American southwest, or further south, and their haplogroup C ancestor was eventually associated with the Spanish. One is from New Mexico. One is from Michigan.

Few of John’s matches have entered the location of their most distant ancestor, but those who have provided that information are shown below at the HVR1 level, understanding that a common ancestor at that level could predate the migration into the Americas.

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Utilizing the information provided through the Genographic project, we find the following information about haplogroup C4c1.

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This provides very interesting geographic distribution information, but it also begs the question of how haplogroup C4c1 was found in Germany or Sweden. Of course, we are relying on participant-reported information and it’s certainly possible that two individuals misunderstood the directions. It’s also possible that one or both are legitimate. I have wondered for a long time about a link between the northern Scandinavian populations, especially subarctic, and the Native subarctic populations in North America.

According to Dr. Doron Behar in the supplement to his paper titled, “A Copernican” Reassessment of the Human Mitochondrial DNA Tree from its Root,” haplogroup C4c1 was born about 10,095 years ago with a standard deviation of 4550 years, meaning the range of time in which C4c1 was born in likely 5,545 to 14,645 years ago. Clearly, there is enough latitude in this date range for some C4c1 to be found in either Asia or Europe, and C4c1 to be found in the Americas as well. If this is indeed the case, one would expect for the variants of C4c1 found on the differing continents to contain a significant difference in mutations, exceeding the 4 mutations allowed for genealogical matching purposes at Family Tree DNA.

To date, there has been no ancient DNA recovered bearing this haplogroup.

Other Mitochondrial Results

Individuals descending from several of John’s maternal lines would be perfect candidates to test for the mitochondrial DNA of those lines. One must descend from these women through all females to the current generation:

  • Follows
  • Esther Ward – Nellie “Pretty Voice” – Beautiful Hail or White Calf
  • Ellen (wife of Joseph Hale)

Testing a female descended through Pretty Voice, mother of Esther Ward, would determine whether or not White Calf was the mother of Pretty Voice, or if it was another woman, probably Beautiful Hail.

John’s Y DNA

John inherited his Y DNA chromosome from Charley White Weasel.

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John’s Y haplogroup is Q-M242, a Native haplogroup.

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John tested to the 67 marker level, but has no matches at 67 markers. At 12, 25 and 37 markers, he matches a gentleman whose ancestor was from Fort Thomson, SD who also tested at 67 markers. That is John’s only match, so apparently John carries some unusual mutations in his Y DNA as well that are probably isolated to people from the Sioux tribe or their ancestors in the past a few hundred to thousands of years.

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On the map above, John’s match is shown and on the map below, John’s white balloon is shown where he was born in relation to that of his red balloon match.

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To obtain additional information about John’s Y DNA haplogroup, the Big Y test would need to be run on his sample. By running the Big Y, we could obtain a more granular haplogroup, meaning further down the tree, and we could also see who matches him more distantly, meaning further back in time. That information could well provide us with information indicating which groups of Native people John is most closely related to. That suggests a migration route or pathway and tells us about social interactions at some level hundreds to thousands of years in the past.

Anzick Child’s Y DNA haplogroup is Q-L54, a subgroup of Q-M242, shown on the haplotree below. You can also see that many subgroups below L54 have been discovered.

im-hap-q-tree

I strongly suspect that John’s haplogroup would be Q-L54 or a subgroup further downstream. I’m betting on a subgroup, meaning that mutations have occurred in John’s line that define a newer, younger haplogroup since the time that Anzick Child and John shared a common ancestor.

Other Y Line Results

I was hopeful that I would find results for John’s Ward or Hale line in the projects at Family Tree DNA, but I did not. I checked in the American Indian project for Hump, with the hope that one of his descendants has tested as well, but did not find that Hump is yet represented in the data base. Of course, anyone paternally descended from Hump’s father, Iron Bull or his father, Black Buffalo would carry the same Y DNA.

If anyone descends from these direct Y lines, please do let us know.

Summary

What we have been able to discover about John’s ancestry both through traditional genealogy and genetic genealogy has been both amazing and fascinating.

John now knows that he is connected to the Anzick Child, the Ancient One. John’s ancestors and Anzick’s were one and the same. Some 12,500 years later, John was born on the same land where his ancestors have literally lived “forever.”

Anzick has given John a wonderful gift, and John has given that gift to the rest of us. We continue to learn through both John and Anzick’s contributions. Thank you to both.

What’s Next?

I would very much like to upgrade John’s Y DNA to 111 markers and order a Big Y test while the holiday sale is in effect. If you would like to contribute to these tests of discovery, please donate to the American Indian project general fund at this link. If we raise more than we need for John’s tests, we have implemented an application process for other Native people. Every donation helps, and helps to build our knowledge base – so please contribute if you can.

Acknowledgements

My gratitude to the following people:

John Iron Moccasin for testing, providing family information and allowing us to work with and publish his results.

John’s mother, Martha Hale, for providing the original genealogical information, below.

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Johns’ friend, Pam, for bringing us this opportunity.

John’s wife, Carolyn, for coordinating information.

Family Tree DNA for testing and facilitating the Ancient DNA Project, the American Indian Project and various Native American haplogroup projects.

nat-geo-logoThe National Geographic Society Genographic Project for providing data base access to the project administrators of the American Indian Project as Affiliate Researchers

Project members and others for contributions to facilitate John’s testing.

My American Indian project co-administrators, Marie Rundquist and Dr. David Pike for their never-failing support.