Which DNA Test Should I Buy? And Why?

Which DNA test should I buy, and why?

I receive questions like this often. As a reminder, I don’t take private clients anymore, which means I don’t provide this type of individual consulting or advice. However, I’m doing the next best thing! In this article, I’m sharing the step-by-step process that I utilize to evaluate these questions so you can use the process too.

It’s important to know what questions to ask and how to evaluate each situation to arrive at the best answer for each person.

Here’s the question I received from someone I’ll call John. I’ve modified the wording slightly and changed the names for privacy.

I’m a male, and my mother was born in Charleston, SC. My maternal grandmother’s maiden name was Jones and a paternal surname was Davis. The family was supposed to have been Black, Dutch, Pennsylvania Dutch, and Scots-Irish…only once was I told I was 3/16 Indian, with Davis being 3/4 and Jones being full Indian.

Do I have enough reasonable information to buy a test, and which one?

Please note that it’s common for questions to arrive without all the information you need to provide a sound answer – so it’s up to you to ask those questions and obtain clarification.

Multiple Questions

There are actually multiple questions here, so let me parse this a bit.

  1. John never mentioned what his testing goal was.
  2. He also never exactly said how the paternal line of Davis was connected, so I’ve made an assumption. For educational purposes, it doesn’t matter because we’re going to walk through the evaluation process, which is the same regardless.
  3. John did not include a tree or a link to a tree, so I created a rudimentary tree to sort through this. I need the visuals and normally just sketch it out on paper quickly.
  4. Does John have enough information to purchase a test?
  5. If so, which test?

There is no “one size fits all” answer, so let’s discuss these one by one.

Easy Answers First

The answer to #4 is easy.

Anyone with any amount of information can purchase a DNA test. Adoptees do it all the time, and they have no prior information.

So, yes, John can purchase a test.

The more difficult question is which test, because that answer depends on John’s goals and whether he’s just looking for some quick information or really wants to delve into genealogy and learn. Neither approach is wrong.

Many people think they want a quick answer –  and then quickly figure out that they really want to know much more about their ancestors.

I wrote an article titled DNA Results – First Glances at Ethnicity and Matching for new testers, here.

Goals

Based on what John said, I’m going to presume his goals are probably:

  • To prove or disprove the family oral history of Black, Dutch, Pennsylvania Dutch (which is actually German,) Scots-Irish, and potentially Native American.
  • John didn’t mention actual genealogy, which would include DNA matches and trees, so we will count that as something John is interested in secondarily. However, he may need genealogy records to reach his primary goal.

If you’re thinking, “The process of answering this seemingly easy question is more complex than I thought,” you’d be right.

Ethnicity in General

It sounds like John is interested in ethnicity testing. Lots of people think that “the answer” will be found there – and sometimes they are right. Often not so much. It depends.

The great news is that John really doesn’t need any information at all to take an autosomal DNA test, and it doesn’t matter if the test-taker is male or female.

To calculate each tester’s ethnicity, every testing company compiles their own reference populations, and John will receive different results at each of the major companies. Each company updates their ethnicity results from time to time as well, and they will change.

Additionally, each company provides different tools for their customers.

The ethnicity results at different companies generally won’t match each other exactly, and sometimes the populations look quite different.

Normally, DNA from a specific ancestor can be found for at least 5 or 6 generations. Of course, that means their DNA, along with the DNA from all of your other ancestors is essentially combined in a communal genetic “pot” of your chromosomes, and the DNA testing company needs to sort it out and analyze your DNA for ethnicity.

DNA descended from ancestors, and their populations, further back in people’s trees may not be discerned at all using autosomal DNA tests.

A much more specific “ethnicity” can be obtained for both the Y-DNA line, which is a direct patrilineal line for men (blue arrow,) and the mitochondrial DNA line (pink arrows,) which is a direct matrilineal line for everyone, using those specific tests.

We will discuss both of those tests after we talk about the autosomal tests available from the four major genealogy DNA testing companies. All of these tools can and should be used together.

Let’s Start with Native American

Let’s evaluate the information that John provided.

John was told that he “was 3/16 Indian, with Davis being 3/4 and Jones being full Indian.”

We need to evaluate this part of his question slightly differently.

I discussed this in the article, Ancestral DNA Percentages – How Much of Them is in You?

First, we need to convert generations to 16ths.

You have two ancestors in your parent’s generation, four in your grandparents, and so forth. You have 16 great-great-grandparents. So, if John was 3/16th Native, then three of his great-great-grandparents would have been fully Native, or an equivalent percentage. In other words, six ancestors in that generation could have been half-Native. Based on what John said, they would have come from his mother’s side of the tree. John is fortunate to have that much information to work with.

He told us enough about his tree that we can evaluate the statement that he might be 3/16ths Native.

Here’s the tree I quickly assembled in a spreadsheet based on John’s information.

His father, at left, is not part of the equation based on the information John provided.

On his mother’s side, John said that Grandfather Davis is supposed to be three-quarters Native, which translates to 12/16ths. Please note that it would be extremely beneficial to find a Y-DNA tester from his Davis line, like one of his mother’s brothers, for example.

John said that his Grandmother Jones is supposed to be 100% Native, so 16/16ths.

Added together, those sum to 28/32, which reduces down to 14/16th or 7/8th for John’s mother.

John would have received half of his autosomal DNA from his mother and half from his non-Native father. That means that if John’s father is 100% non-Native, John would be half of 14/16ths or 7/16ths, so just shy of half Native.

Of course, we know that we don’t always receive exactly 50% of each of our ancestors’ DNA (except for our parents,) but we would expect to see something in the ballpark of 40-45% Native for John if his grandmother was 100% Native and his grandfather was 75%.

Using simple logic here, for John’s grandmother to be 100% Native, she would almost assuredly have been a registered tribal member, and the same if his grandfather was 75% Native. I would think that information would be readily available and well-known to the family – so I doubt that this percentage is accurate. It would be easy to check, though, on various census records during their lifetimes where they would likely have been recorded as “Indian.” They might have been in the special “Indian Census” taken and might be living on a reservation.

It should also be relatively easy to find their parents since all family members were listed every ten years in the US beginning with the 1850 census.

The simple answer is that if John’s grandparents had as much Native as reported, he would be more than 3/16th – so both of these factoids cannot simultaneously be accurate. But that does NOT mean neither is accurate.

John could be 7/8th or 40ish%, 3/16th or 18ish%, or some other percentage. Sometimes, where there is smoke, there is fire. And that seems to be the quandary John is seeking to resolve.

Would  Ethnicity/Population Tests Show This Much Native?

Any of the four major testing companies would show Native for someone whose percentage would be in the 40% or 18% ballpark.

The easiest ethnicities to tell apart from one another are continental-level populations. John also stated that he thinks he may also have Black ancestry, plus Dutch, Pennsylvania Dutch (German), and Scots-Irish. It’s certainly possible to verify that using genealogy, but what can DNA testing alone tell us?

How far back can we expect to find ethnicities descending from particular ancestors?

In this table, you can see at each generation how many ancestors you have in that generation, plus the percentage of DNA, on average, you would inherit from each ancestor.

All of the major DNA testing companies can potentially pick up small trace percentages, but they don’t always. Sometimes one company does, and another doesn’t. So, if John has one sixth-generation Native American ancestor, he would carry about 1.56% Native DNA, if any.

  • Sometimes a specific ethnicity is not found because, thanks to random recombination, you didn’t inherit any of that DNA from those ancestors. This is why testing your parents, grandparents, aunts, uncles, and siblings can be very important. They share your same ancestors and may have inherited DNA that you didn’t that’s very relevant to your search.
  • Sometimes it’s not found because the reference populations and algorithms at that testing company aren’t able to detect that population or identify it accurately, especially at trace levels. Every DNA testing company establishes their own reference populations and writes internal, proprietary ethnicity analysis algorithms.
  • Sometimes it’s not found because your ancestor wasn’t Native or from that specific population.
  • Sometimes it’s there, but your population is called something you don’t expect.

For example, you may find Scandinavian when your ancestor was from England or Ireland. The Vikings raided the British Isles, so while some small amount of Scandinavian is not what you expect, that doesn’t mean it‘s wrong. However, if all of your family is from England, it’s not reasonable to have entirely Scandinavian ethnicity results.

It’s also less likely as each generation passes by that the information about their origins gets handed down accurately to following generations. Most non-genealogists don’t know the names of their great-grandparents, let alone where their ancestors were from.

Using a 25-year average generation length, by the 4th generation, shown in the chart above, you have 16 ancestors who lived approximately 100 years before your parents were born, so someplace in the mid-1800s. It’s unlikely for oral history from that time to survive intact. It’s even less likely from a century years earlier, where in the 7th generation, you have 128 total ancestors.

The best way to validate the accuracy of your ethnicity estimates is by researching your genealogy. Of course, you need to take an ethnicity test, or two, in order to have results to validate.

Ethnicity has a lot more to offer than just percentages.

Best Autosomal Tests for Native Ethnicity

Based on my experience with people who have confirmed Native ancestry, the two best tests to detect Native American ethnicity, especially in smaller percentages, are both FamilyTreeDNA and 23andMe.

Click images to enlarge

In addition to percentages, both 23andMe and FamilyTreeDNA provide chromosome painting for ethnicity, along with segment information in download files. In other words, they literally paint your ethnicity results on your chromosomes.

They then provide you with a file with the “addresses” of those ethnicities on your chromosomes, which means you can figure out which ancestors contributed those ethnicity segments.

The person in the example above, a tester at FamilyTreeDNA, is highly admixed with ancestors from European regions, African regions and Native people from South America.

Trace amounts of Native American with a majority of European heritage would appear more like this.

You can use this information to paint your chromosome segments at DNAPainter, along with your matching segments to other testers where you can identify your common ancestors. This is why providing trees is critically important – DNA plus ancestor identification with our matches is how we confirm our ancestry.

This combination allows you to identify which Native (or another ethnicity) segments descended from which ancestors. I was able to determine which ancestor provided that pink Native American segment on chromosome 1 on my mother’s side.

I’ve provided instructions for painting ethnicity segments to identify their origins in specific ancestors, here.

Autosomal and Genealogy

You may have noticed that we’ve now drifted into the genealogy realm of autosomal DNA testing. Ethnicity is nice, but if you want to know who those segments came from, you’ll need:

  • Autosomal test matching to other people
  • To identify your common ancestor with as many matches as you can
  • To match at a company who provides you with segment information for each match
  • To work with DNAPainter, which is very easy

The great news is that you can do all of that using the autosomal tests you took for ethnicity, except at Ancestry who does not provide segment information.

Best Autosomal Test for Matching Other Testers

The best autosomal test for matching may be different for everyone. Let’s look at some of the differentiators and considerations.

If you’re basing a testing recommendation solely on database size, which will probably correlate to more matches, then the DNA testing vendors fall into this order:

If you’re basing that recommendation on the BEST, generally meaning the closest matches for you, there’s no way of knowing ahead of time. At each of the four DNA testing companies, I have very good matches who have not tested elsewhere. If I weren’t in all four databases, I would have missed many valuable matches.

If you’re basing that recommendation on which vendor began testing earliest, meaning they have many tests from people who are now deceased, so you won’t find their autosomal tests in other databases that don’t accept uploads, the recommended testing company order would be:

If you’re basing that recommendation on matches to people who live in other countries, the order would be:

Ancestry and 23andMe are very distant third/fourth because they did not sell widely outside the US initially and still don’t sell in as many countries as the others, meaning their testers’ geography is more limited. However, Ancestry is also prevalent in the UK.

If you’re basing that recommendation on segment information and advanced tools that allow you to triangulate and confirm your genetic link to specific ancestors, the order would be:

Ancestry does NOT provide any segment information.

If you’re basing that recommendation on unique tools provided by each vendor, every vendor has something very beneficial that the others don’t.

In other words, there’s really no clear-cut answer for which single autosomal DNA test to order. The real answer is to be sure you’re fishing in all the ponds. The fish are not the same. Unique people test at each of those companies daily who will never be found in the other databases.

Test at or upload your DNA to all four DNA testing companies, plus GEDmatch. Step-by-step instructions for downloading your raw data file and uploading it to the DNA testing companies who accept uploads can be found, here.

Test or Upload

Not all testing companies accept uploads of raw autosomal DNA data files from other companies. The good news is that some do, and it’s free to upload and receive matches.

Two major DNA testing companies DO NOT accept uploads from other companies. In other words, you have to test at that company:

Two testing companies DO accept uploads from the other three companies. Uploads and matching are free, and advanced features can be unlocked very cost effectively.

  • FamilyTreeDNA – free matching and $19 unlock for advanced features
  • MyHeritage – free matching and $29 unlock.for advanced features

I recommend testing at both 23andMe and Ancestry and uploading one of those files to both FamilyTreeDNA and MyHeritage, then purchasing the respective unlocks.

GEDmatch

GEDmatch is a third-party matching site, not a DNA testing company. Consider uploading to GEDmatch because you may find matches from Ancestry who have uploaded to GEDmatch, giving you access to matching segment information.

Other Types of DNA

John provided additional information that may prove to be VERY useful. Both Y-DNA and mitochondrial DNA can be tested as well and may prove to be more useful than autosomal to positively identify the origins of those two specific lines.

Let’s assume that John takes an autosomal test and discovers that indeed, the 3/16th Native estimate was close. 3/16th equates to about 18% Native which would mean that three of his 16 great-great-grandparents were Native.

John told us that his Grandmother Jones was supposed to be 100% Native.

At the great-great-grandparent level, John has 16 ancestors, so eight on his mother’s side, four from maternal grandmother Jones and four from his maternal grandfather Davis.

John carries the mitochondrial DNA of his mother (red boxes and arrows,) and her mother, through a direct line of females back in time. John also carries the Y-DNA of his father (dark blue box, at left above, and blue arrows below.)

Unlike autosomal DNA which is admixed in every generation, mitochondrial DNA (red arrows) is inherited from that direct matrilineal line ONLY and never combines with the DNA of the father. Mothers give their mitochondrial DNA to both sexes of their children, but men never contribute their mitochondrial DNA to offspring. Everyone has their mother’s mitochondrial DNA.

Because it never recombines with DNA from the father, so is never “watered down,” we can “see” much further back in time, even though we can’t yet identify those ancestors.

However, more importantly, in this situation, John can test his own mitochondrial DNA that he inherited from his mother, who inherited it from her mother, to view her direct matrilineal line.

John’s mitochondrial DNA haplogroup that will be assigned during testing tells us unquestionably whether or not his direct matrilineal ancestor was Native on her mother’s line, or not. If not, it may well tell us where that specific line originated.

You can view the countries around the world where Y-DNA haplogroups are found, here, and mitochondrial haplogroups, here.

If John’s mitochondrial DNA haplogroup is Native, that confirms that one specific line is Native. If he can find other testers in his various lines to test either their Y-DNA or mitochondrial DNA, John can determine if other ancestors were Native too. If not, those tests will reveal the origins of that line, separate from the rest of his genealogical lines.

Although John didn’t mention his father’s line, if he takes a Y-DNA test, especially at the Big Y-700 level, that will also reveal the origins of his direct paternal line. Y-DNA doesn’t combine with the other parent’s DNA either, so it reaches far back in time too.

Y-DNA and mitochondrial DNA tests are laser-focused on one line each, and only one line. You don’t have to try to sort it out of the ethnicity “pot,” wondering which ancestor was or was not Native.

My Recommendation

When putting together a testing strategy, I recommend taking advantage of free uploads and inexpensive unlocks when possible.

  • To confirm Native American ancestry via ethnicity testing, I recommend testing at 23andMe and uploading to FamilyTreeDNA, then purchasing the $19 unlock. The free upload and $19 unlock are less expensive than testing there directly.
  • For matching, I recommend testing at Ancestry and uploading to MyHeritage, then unlocking the MyHeritage advanced features for $29, which is less expensive than retesting. Ancestry does not provide segment information, but MyHeritage (and the others) do.

At this point, John will have taken two DNA tests, but is now in all four databases, plus GEDmatch if he uploads there.

  • For genealogy research on John’s lines to determine whether or not his mother’s lines were Native, I recommend an Ancestry and a MyHeritage records subscription, plus using WikiTree, which is free.
  • To determine if John’s mother’s direct matrilineal female line was Native, I recommend that John order the mitochondrial DNA test at FamilyTreeDNA.
  • When ordering multiple tests, or uploading at FamilyTreeDNA, be sure to upload/order all of one person’s tests on the same DNA kit so that those results can be used in combination with each other.

Both males and females can take autosomal and mitochondrial DNA tests.

  • To discover what he doesn’t know about his direct paternal, meaning John’s surname line – I recommend the Big Y-700 test at FamilyTreeDNA.

Only males can take a Y-DNA test, so women would need to ask their father, brother, or paternal uncle, for example, to test their direct paternal line.

  • If John can find a male Davis from his mother’s line, I recommend that he purchase the Big Y-700 test at FamilyTreeDNA for that person, or check to see if someone from his Davis line may have already tested by viewing the Davis DNA Project. Like with mitochondrial DNA, the Y-DNA haplogroup will tell John the origins of his direct Davis male ancestor – plus matching of course. He will be able to determine if they were Native, and if not, discover the origins of the Davis line.
  • For assigning segments to ancestors and triangulating to confirm descent from a common ancestor, I recommend 23andMe, MyHeritage, FamilyTreeDNA and GEDmatch, paired with DNAPainter as a tool.

Shopping and Research List

Here are the tests and links recommended above:

More Than He Asked

I realize this answer is way more than John expected or even knew to ask. That’s because there is often no “one” or “one best” answer. There are many ways to approach the question after the goal is defined, and the first “answer” received may be a bit out of context.

For example, let’s say John has 2% Native ancestry and took a test at a vendor who didn’t detect it. John would believe he had none. But a different vendor might find that 2%. If it’s on his mother’s direct matrilineal line, mitochondrial DNA testing will confirm, or refute Native, beyond any doubt, regardless of autosomal ethnicity results – but only for that specific ancestral line.

Autosomal DNA can suggest Native across all your DNA, but Y-DNA and mitochondrial DNA confirm it for each individual ancestor.

Even when autosomal testing does NOT show Native American, or African, for example, it’s certainly possible that it’s just too far back in time or has not been passed down during random recombination, but either Y-DNA or mitochondrial DNA will unquestionably confirm (or refute) the ancestry in question if the right person is tested.

This is exactly why I attempt to find a cousin who descends appropriately from every ancestor and provide testing scholarships. It’s important to obtain Y-DNA and mitochondrial DNA information for each ancestor.

Which Test Should I Order?

What steps will help you decide which test or tests to take?

  1. Define your testing goal.
  2. Determine if your Y-DNA or mitochondrial DNA will help answer the question.
  3. Determine if you need to find ancestors another generation or two back in time to get the most benefit from DNA testing. In our example, if John discovered that both of his grandparents were enrolled tribal members, that’s huge, and the tribe might have additional information about his family.
  4. Subscribe to Ancestry and MyHeritage records collections as appropriate to perform genealogical research. Additional information not only provides context for your family, it also provides you with the ability to confirm or better understand your ethnicity results.
  5. Extend your tree so that you can obtain the best results from the three vendors who support trees; Ancestry, FamilyTreeDNA, and MyHeritage. All three use trees combined with DNA tests to provide you with additional information.
  6. Order 23andMe and Ancestry autosomal DNA tests.
  7. Either test at or upload one of those tests to MyHeritage, FamilyTreeDNA, and GEDmatch.
  8. If a male, order the Big Y-700 DNA test. Or, find a male from your ancestral line who has taken or will take that test. I always offer a testing scholarship and, of course, share the exciting results!
  9. Order a mitochondrial DNA test for yourself and for appropriately descended family members to represent other ancestors. Remember that your father (and his siblings) all carry your paternal grandmother’s mitochondrial DNA. That’s often a good place to start after testing your own DNA.
  10. If your parents or grandparents are alive, or aunts and uncles, test their autosomal DNA too. They are (at least) one generation closer to your ancestors than you are and will carry more of your ancestors’ DNA.
  11. Your siblings will carry some of your ancestors’ DNA that you do not, so test them too if both of your parents aren’t available for testing.

Enjoy!!!

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WikiTree Connections, King Charles III, and DNA

By Copyright House of Lords 2022 / Photography by Annabel Moeller, CC BY 2.0, https://commons.wikimedia.org/w/index.php?curid=117865820

I’m not a royal-watcher, but you’d pretty much have to be dead to not be aware that King Charles III is being crowned this Saturday, May 6th.

Have you wondered if you’re related to Charles? Or someone else?

It’s easy to find out on WikiTree.

Go to King Charles’s profile, here.

Notice that under “DNA Connections,” a WikiTree user has entered the Y-DNA of the line of King Charles via an academic sample uploaded to mitoYDNA. That’s interesting!

Tsar Romanov and King Charles III both descend from a common ancestor and are first cousins twice removed (1C2R.) You can also see more about Nicholas Romanov II in the FamilyTreeDNA Discover tool under haplogroup R-M269, in Notable Connections.

Under WikiTree DNA Connections, I notice no one has entered King Charles’s mitochondrial DNA information. Of course, King Charles inherited his mtDNA from his mother, Queen Elizabeth II.

If you know of anyone who carries Queen Elizabeth’s mitochondrial DNA through her direct matrilineal ancestors, by all means, enter this information. If you don’t know how, you can click on help at the bottom of the page or click here. WikiTree has lots of truly helpful volunteers.

You can also enter your information if you’ve taken an autosomal, Y-DNA, or mitochondrial DNA test and are descended appropriately from the person represented in the profile.

Here’s an example from my ancestor, Phebe Cole’s profile. I entered where I tested, and my GEDmatch number.

You can add your DNA test information by clicking on the “Add” button in the top header, then DNA Test Information here.

WikiTree DNA Benefits

WikiTree is a wonderful place to:

  • Upload your DNA to the relevant profile, where it will be populated up the tree appropriately
  • Obtain DNA information, including haplogroups, about your ancestors
  • Discover cousins who descend from that ancestor and who have tested their DNA
  • Discover cousins who may not have tested yet, but might be willing

I use WikiTree regularly to fish for Y and mitochondrial DNA information about my ancestors and to see if I match cousins listed as descendants of a common ancestor.

WikiTree works in the opposite direction from the DNA testing vendors.

At the testing vendors, you find the match and then need to determine how they are related. At WikiTree, you check your ancestor and will find a list of cousins who descend from that ancestor and who have DNA tested. You already know at least one way that each person is related to you. Finding cousin matches by ancestor is part of my triangulation process.

Are You Related?

No known DNA testers or don’t match – no problem.

You can determine whether or not you’re genealogically related to any individual on Wikitree.

Just sign in to your account, and select the profile of the person you want to check.

Scroll very near the bottom or do a browser search for the words “your connection.”

Just click on “Your connection” or “Your genealogical relationship.”

Collaborate is Key

WikiTree is crowd-sourced, so be sure to verify your connection pathway results. If the path isn’t accurate, you can correct the inaccurate person or connection. We are all doing the genealogy community a HUGE favor by ensuring this collaborative tree is accurate.

If you’re unsure about a connection, check the sources and evidence for each generation. If you need information, contact the profile manager.

Add a comment, ask a question, add an image, or provide additional information and sources on any profile.

Ancestral Legacy

I regularly update my ancestors’ profiles with additional information when it becomes available. I appreciate everything others have shared with me over the years, and I want to be sure the information about my ancestors is as accurate as possible.

I don’t know about you, but I’m in this for the long game – for posterity. Leaving as much accurate information, including Y and mitochondrial DNA, is the very least I can do for my ancestors. After all, we wouldn’t be here without them.

So, are you related to King Charles? Is your distant cousin being crowned on Saturday?

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You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an email whenever I publish by clicking the “follow” button on the main blog page, here.

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I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Uploads

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DNA: In Search Of…Your Grandparents

Are you searching for an unknown relative or trying to unravel and understand unexpected results? Maybe you discovered that one or both of your parents is not your biological parent. Maybe one of your siblings might be a half-sibling instead. Or maybe you suddenly have an unexpected match that looks to be an unknown close relative, possibly a half-sibling. Perhaps there’s a close match you can’t place.

Or, are you searching for the identity of your grandparent or grandparents? If you’re searching for your parent or parents, often identifying your grandparents is a necessary step to narrow the parent-candidates.

I’ve written an entire series of “In Search of Unknown Family” articles, permanently listed together, here. They will step you through the search process and help you understand how to unravel your results. If you’re new, reading these, in order, before proceeding, would be a good idea.

Identifying a Grandparent

I saved this “grandparents” article for later in the series because you will need the tools and techniques I’ve introduced in the earlier articles. Identifying grandparents is often the most challenging of any of the relationships we’ve covered so far. In part because each of those four individuals occupies a different place in your tree, meaning their X, Y-DNA and mitochondrial DNA is carried by different, and not all, descendants. This means we sometimes have to utilize different tools and techniques.

If you’re trying to identify any of your four grandparents, females are sometimes more challenging than males.

Why?

Women don’t have a Y chromosome to test. This can be a double handicap. Female testers can’t test a Y chromosome, and maternal ancestors don’t have a Y chromosome to match.

Of course, every circumstance differs. You may not have a male to test for paternal lines either.

The maternal grandfather can be uniquely challenging, because two types of DNA, Y-DNA and mitochondrial DNA matching are immediately eliminated for all testers.

While I’ve focused on the maternal grandfather in this example, these techniques can be utilized for all four grandparents as well as for parents. At the end, I’ll review other grandparent relationships and additional tools you might be able to utilize for each one.

In addition to autosomal DNA, we can also utilize mitochondrial DNA, Y-DNA and sometimes X DNA in certain situations.

Testing, Tests and Vendors

As you recall, only men have a Y chromosome (blue arrow), so only genetic males can take a Y-DNA test. Men pass their Y chromosome from father to son in each generation. Daughters don’t receive a Y chromosome.

Everyone has their mother’s mitochondrial DNA (pink arrow.) Women pass their mitochondrial DNA to both sexes of their children, but only females pass it on. In the current generation, represented by the son and daughter, above, the mother’s yellow heart-shaped mitochondrial DNA is inherited by both sexes of her children. In the current generation, males and females can both test for their mother’s mitochondrial DNA.

Of course, everyone has autosomal DNA, inherited from all of their ancestral lines through at least the 5th or 6th generation, and often further back in time. Autosomal DNA is divided in half in each generation, as children inherit half of each parents’ autosomal DNA (with the exception of the X chromosome, which males only inherit from their mother.)

The four major vendors, Ancestry, 23andMe, FamilyTreeDNA and MyHeritage sell autosomal DNA tests, but only FamilyTreeDNA sells Y-DNA and mitochondrial DNA tests.

Only 23andMe and FamilyTreeDNA report X matching.

All vendors except Ancestry provide segment location information along with a chromosome browser.

You can read about the vendor’s strengths and weaknesses in the third article, here.

Ordering Y and Mitochondrial DNA Tests

If you’re seeking the identities of grandparents, the children and parents, above, can test for the following types of DNA in addition to autosomal:

Person in Pedigree Y-DNA Mitochondrial
Son His father’s blue star His mother’s pink heart
Daughter None Her mother’s pink heart
Father His father’s blue star His mother’s gold heart
Mother None Her mother’s pink heart

Note that none of the people shown above in the direct pedigree line carry the Y-DNA of the green maternal grandfather. However, if the mother has a full sibling, the green “Male Child,” he will carry the Y-DNA of the maternal grandfather. Just be sure the mother and her brother are full siblings, because otherwise, the brother’s Y-DNA may not have been inherited from your mother’s father. I wrote about full vs half sibling determination, here.

Let’s view this from a slightly different perspective. For each grandparent in the tree, which of the two testers, son or daughter, if either, carry that ancestor’s DNA of the types listed in the columns.

Ancestor in Tree Y-DNA Mitochondrial DNA Autosomal DNA X DNA
Paternal Grandfather Son Neither Son, daughter Neither
Paternal Grandmother Has no Y chromosome None (father has it, doesn’t pass it on to son or daughter) Son, daughter Daughter (son does not receive father’s X chromosome)
Maternal Grandfather Neither Neither Son, daughter Son, daughter (potentially)
Maternal Grandmother Has no Y chromosome Son, daughter Son, daughter Son, daughter (potentially)

Obtaining the Y-DNA and mitochondrial DNA of those grandparents from their descendants will provide hints and may be instrumental in identifying the grandparent.

FamilyTreeDNA

You’ll need to order Y-DNA (males only) and mitochondrial DNA tests separately from autosomal DNA tests. They are three completely different tests.

At FamilyTreeDNA, the autosomal DNA test is called Family Finder to differentiate it from their Y-DNA and mitochondrial DNA tests.

Their autosomal test is called Family Finder whether you order a test from FamilyTreeDNA, or upload your results to their site from another vendor (instructions here.)

I recommend ordering the Big Y-700 Y-DNA test if possible, and if not, the highest resolution Y-DNA test you can afford. The Big Y-700 is the most refined Y-DNA test available, includes multiple tools and places Big Y-700 testers on the Time Tree through the Discover tool, providing relatively precise estimates of when those men shared a common ancestor. If you’ve already purchased a lower-precision Y-DNA test at FamilyTreeDNA, you can easily upgrade.

I wrote about using the Discover tool here. The recently added Group Time Tree draws a genetic Y-DNA tree of Big-Y testers in common projects, showing earliest known ancestors and the date of the most recent common ancestor.

You need to make sure your Family Finder, mitochondrial DNA and Y-DNA (if you’re a male) tests are ordered from the same account at FamilyTreeDNA.

You want all 3 of your tests on the same account (called a kit number) so that you can use the advanced search features that display people who match you on combinations of multiple kinds of tests. For example, if you’re a male, do your Y-DNA matches also match you on the autosomal Family Finder test, and if so, how closely? Advanced matching also provides X matching tools.

X DNA is included in autosomal tests. X DNA has a distinct matching pattern for males and females which makes it uniquely useful for genealogy. I wrote about X DNA matching here.

If you upload your autosomal results to FamilyTreeDNA from another company, you’re only uploading a raw DNA file, not the DNA itself, so FamilyTreeDNA will need to send you a swab kit to test your Y-DNA and mitochondrial DNA. If you upload your autosomal DNA, simply sign in to your kit, purchase the Y-DNA and/or mitochondrial DNA tests and they will send you a swab kit.

If you test directly at FamilyTreeDNA, you can add any test easily by simply signing in and placing an order. They will use your archived DNA from your swab sample, as long as there’s enough left and it’s of sufficient quality.

Fish In All Ponds

The first important thing to do in your grandparent search is to be sure you’re fishing in all ponds. In other words, be sure you’ve tested at all 4 vendors, or uploaded files to FamilyTreeDNA and MyHeritage.

When you upload files to those vendors, be sure to purchase the unlock for their advanced tools, because you’re going to utilize everything possible.

If you have relatively close matches at other vendors, ask if they will upload their files too. The upload is free. Not only will they receive additional matches, and another set of ethnicity results, their results will help you by associating your matches with specific sides of your family.

Why Order Multiple Tests Now Instead of Waiting?

I encourage testers to order their tests at the beginning of their journey, not one at a time. Each new test from a vendor takes about 6-8 weeks from the time you initially order – they send the test, you swab or spit, return it, and they process your DNA. Of course, uploading takes far less time.

If you’re adding elapsed time, two autosomal tests (Ancestry and 23andMe), two uploads (FamilyTreeDNA and MyHeritage,) a Y-DNA and a mitochondrial DNA test, if all purchased serially, one after the other, means you’ll be waiting about 6-8 months.

Do you want to wait 6-8 months? Can you afford to?

Part of that answer has to do with what, exactly, you’re seeking.

A Name or Information?

Are you seeking the name of a person, or are you seeking information about that person? With grandparents, you may be hoping to meet them, and time may be of the essence. Time delayed may not be able to be recovered or regained.

Most people don’t just want to put a name to the person they are seeking – they want to learn about them. You will have different matches at each company. Even after you identify the person you seek, the people you match at each company may have information about them, their photos, know about their life, family, and their ancestors. They may be able and willing to facilitate an introduction if that’s what you seek.

One cousin that I assisted discovered that his father had died just 6 weeks before he made the connection. He was heartsick.

Having data from all vendors simultaneously will allow you to compile that data and work with it together as well as separately. Using your “best” matches at each company, augmented by both Y-DNA and mitochondrial DNA can make MUCH shorter work of this search.

Your Y-DNA, if you’re a male will give you insights into your surname line, and the Big-Y test now comes with estimates of how far in the past you share a common ancestor with other men that have taken the Big-Y test. This can be a HUGE boon to a male trying to figure out his surname line.

Y-DNA and mitochondrial DNA, respectively, will eliminate many people from being your mother or father, or your direct paternal or direct maternal line ancestor. Both provide insights into which population and where that population originated as well. In other words, it provides you lineage-specific information not available elsewhere.

Your Y-DNA and mitochondrial DNA can also provide critically important information about whether that direct line ancestor belonged to an endogamous population, and where they came from.

Strategies

You may be tempted to think that you only need to test at one vendor, or at the vendor with the largest database, but that’s not necessarily true.

Here’s a table of my closest matches at the 4 vendors.

Vendor Closest Maternal Closest Paternal Comments
Ancestry 1C, 1C1R Half 1C, 2C I recognized both of the maternal and neither of the paternal.
23andMe 2C, 2C 1C1R, half-gr-niece Recognized both maternal, one paternal
MyHeritage Mother uploaded, 1C Half-niece, half 1C Recognized both maternal, one paternal
FamilyTreeDNA Mother tested, 1C1R Parent/child, half-gr-niece Recognized all 4

To be clear, I tested my mother at FamilyTreeDNA before she passed away, but if I was an adoptee searching for my mother, that’s the first database she would be in. As her family, we were able to order the Family Finder test from her archived DNA after she had passed away. I then uploaded her DNA file to MyHeritage, but she’ll never be at either 23andMe or Ancestry because they don’t accept uploads and she clearly can’t test.

Additionally, being able to identify maternal matches by viewing shared matches with my mother separates out close matches from my paternal side.

Let’s put this another way, I stand a MUCH BETTER chance of unraveling this mystery with the combined closest matches of all 4 databases instead of the top ones from just one database.

I’m providing analysis methodologies for working with results from all of the vendors together, in case your answer is not immediately obvious. Taking multiple tests facilitates using all of these tools immediately, not months later. Solving the puzzle sooner means you may not miss valuable connection opportunities.

You may also discover that the door slams shut with some people, but another match may be unbelievably helpful. Don’t unnecessarily limit your possibilities.

Here’s the testing and upload strategy I recommend.

What When Ancestry 23andMe MyHeritage FamilyTreeDNA GEDmatch
Order autosomal test Initially Yes Yes Upload Upload Upload
Order Big-Y DNA test if male Initially Yes
Order mitochondrial DNA test Initially Yes
Upload free autosomal file From Ancestry or 23andMe Yes Yes Yes
Unlock Advanced Tools When upload file $29 $19 $9.95 month
Includes X Matching No Yes No Yes Yes
Chromosome Browser, segment location information No Yes Yes Yes Yes

When you upload a DNA file to a vendor site, only upload one file per site, per tester. Otherwise, multiple tests simply glom up everyone’s match list with multiple matches to the same person and can be very confusing.

  • One person took an autosomal test at a company that accepts uploads, forgot about it, uploaded a file from another vendor later, and immediately thought she had found her parent. She had not. She “found” herself.
  • Another person though she had found two sisters, but one person had uploaded their own file from two different vendors.

Multiple vendor sites reveal multiple close matches to different people which increase your opportunity to discover INFORMATION about your family, not just the identity of the person.

Match Ranges

Given that we are searching for an unknown maternal grandfather, your mother may not have had any (known) full siblings. The “best” match would be to a full or half siblings to your parents, or their descendants, depending on how old your grandparents would be.

Let’s take the “worst case” scenario, meaning there are no full siblings AND there are many possible generations between you and the people you may match.

Now, let’s look at DNAPainter’s Shared cM tool.

You’re going to be looking for someone who is either your mother’s half sibling on her father’s side, or who is a full sibling.

If your mother is adopted, it’s possible that she has or had full siblings. If your mother was born circa 1920, it’s likely that you will be matching the next generation, or two, or three.

However, if your mother was born later, you could be matching her siblings directly.

I’m going to assume half siblings for this example, because they are more difficult than full siblings.

Full sibling relationships for your mother’s siblings are listed at right. Your full aunt or uncle at top, then their descendant generations below.

At left, in red, are the half-sibling relationships and the matching amounts.

You can see that if you’re dealing with half 1C3R (half first cousin three times removed,) you may not match.

Therefore, in order to isolate matches, it’s imperative to test every relevant relative possible.

Who’s Relevant for DNA Testing?

Who is relevant to test If you’re attempting to identify your maternal grandfather?

The goal is to be able to assign matches to the most refined ancestor possible. In other words, if you can assign someone to either your grandmother’s line, or your grandfather’s line, that’s better than assigning the person to your grandparents jointly.

Always utilize the tests of the people furthest up the tree, meaning the oldest generations. Their DNA is less-diluted, meaning it has been divided fewer times. Think about who is living and might be willing to test.

You need to be able to divide your matches between your parents, and then between your grandparents on your mother’s side.

  • Test your parents, of course, and any of their known siblings, half or full.
  • If those siblings have passed away, test as many of their children as you can.
  • If any of your grandparents are living, test them
  • If BOTH of your grandparents on the same side aren’t available to test, test any, preferably all, living aunts or uncles.
  • If your maternal grandmother had siblings, test them or their descendants if they are deceased.
  • If your parents are deceased, test your aunts, uncles, full siblings and half-siblings on your mother’s side. (Personally, I’d test all half-siblings, not just maternal.)
  • Half-siblings are particularly valuable because there is no question which “side” your shared DNA came from. They will match people you don’t because they received part of your parent’s DNA that you did not.

Furthermore, shared matches to half-siblings unquestionably identify which parent those matches are through.

Essentially, you’re trying to account for all matches that can be assigned to your grandparents whose identities you know – leaving only people who descend from your unknown maternal grandfather.

Testing your own descendants will not aid your quest. There is no need to test them for this purpose, given that they received half of your DNA.

I wrote about why testing close relatives is important in the article Superpower: Your Aunts’ and Uncles’ DNA is Your DNA Too – Maximize Those Matches!

Create or Upload a Tree

Three of the four major vendors, plus GEDMatch, support and utilize family trees.

You’ll want to either upload or create a tree at each of the vendor sites.

You can either upload a GEDCOM file from your home computer genealogy software, or you can create a tree at one of the vendors, download it, and upload to the others. I described that process at Ancestry, here.

Goal

Your goal is to work with your highest matches first to determine how they are related to you, thereby eliminating matches to known lineages.

Assuming you’re only searching for the identity of one grandparent, it’s beneficial to have done enough of your genealogy on your three known grandparents to be able to assign matches from those lines to those sides.

Step 1 is to check each vendor for close matches that might fall into that category.

The Top 15 at Each Vendor

Your closest several autosomal matches are the most important and insightful. I begin with the top 15 autosomal results at each vendor, initially, which provides me with the best chance of meaningful close relationship discoveries.

Create a Spreadsheet or Chart

I hate to use that S word (spreadsheet), because I don’t want non-technical people to be discouraged. So, I’m going to show you how I set up a spreadsheet and you can simply create a chart or even draw this out on paper if you wish.

I’ve color-coded columns for each of my 4 grandparents. The green column is the target Maternal Grandfather whose identity I’m seeking.

I match our first example; Erik, at 417 cM. Based on various pieces of information, taken together, I’ve determined that I’m Erik’s half 1C1R. His 8 great-grandparent surnames, or the ones he has provided, indicate that I’m related to Eric on my paternal grandfather’s line.

You’ll want to record your closest matches in this fashion.

Let’s look at how to find this information and work with the tools at the individual vendors.

23andMe

Let’s start at 23andMe, because they create a potential genetic tree for you, which may or may not be accurate.

I have two separate tests at 23andMe. One is a V3 and one is a V4 test. I keep one in its pristine state, and I work with the second one. You’ll see two of “me” in the tree, and that’s why.

23andMe makes it easy to see estimated relationships, although they are not always correct. Generally, they are close, and they can be quite valuable.

Click on any image to enlarge

The maternal and paternal “sides” may not be positioned where genealogists are used to seeing them. Remember, 23andMe has no genealogy trees, so they are attempting to construct a genetic tree based on how people are related to you and to each other, with no prior knowledge. They do sometimes have issues with half-relationships, so I’d encourage you to use this tree to isolate people to the three grandparents you know.

In my case, I was able to determine the maternal and paternal sides easily based on known cousins. This is the perfect example of why it’s important to test known relatives from both sides of your family.

My paternal side, at right, in blue, was easy because I recognized my half-sister’s family, and because of known cousins who I recognized from having tested elsewhere. I’ve worked with them for years. The blue stars show people I could identify, mostly second cousins.

My maternal side is at left, in red. Normally, for genealogists, the maternal side is at right, and the paternal at left, so don’t make assumptions, and don’t let this positioning throw you.

I’m pretending I don’t know who my maternal grandfather is. I was able to identify my maternal grandmother’s side based on a known second cousin.

That leaves my target – my maternal grandfather’s line.

All of the matches to the left of the red circle would, by process of elimination, be on my maternal grandfather’s side.

The next step would be to figure out how the 5 people descending from my maternal grandfather’s line are related to each other – through which of their ancestors.

On the DNA Relatives match list, here’s what needs to be checked:

  • Do your matches share surnames with you or your ancestors?
  • Do they show surnames in common with each other?
  • Is there a common location?
  • Birth year which helps you understand their potential generation.
  • Did they list their grandparents’ birthplaces?
  • Did they provide a family tree link?
  • Do they also match each other using the Relatives in Common feature?
  • Do they triangulate, indicated by “DNA Overlap” in Relatives in Common?
  • Who else is on the Relatives in Common list, and what do they have in common with each other?
  • Looking at your Ancestry Composition compared with theirs, what are your shared populations, and are they relevant? If you are both 100% European, then shared populations aren’t useful, but if both people share the same minority ancestry, especially on the same segments, it may indeed be relevant – especially if it can’t be accounted for on the known sides of the family.

Reach out to these people and see what they know about their genealogy, if they have tested elsewhere, and if they have a genealogy tree someplace that you can view.

If they can tell you their grandparents’ names, birth and death dates and locations, you can check public sources like WikiTree, FamilySearch and Geni, or build trees for them. You can also use Newspaper resources, like Newspapers.com, NewspaperArchive and the newspapers at MyHeritage.

I added the top 15 23andMe matches into the spreadsheet I created.

You’ll notice that not many people at 23andMe enter surnames. However, if you can identify individuals from your 3 known lines, you can piggyback the rest by using Relatives in Common in conjunction with the genetic tree placement.

Be sure to check all the people that are connected to the target line in your genetic tree.

You’ll want to harvest your DNA segments to paint at DNAPainter if you don’t solve this mystery with initial reviews at each vendor.

Ancestry

Let’s move to Ancestry next.

At Ancestry, you’ll want to start with your closest matches on your match list.

Ancestry classifies “Close Matches” as anyone 200 cM or greater, which probably won’t reach as far down as the matches we’ll want to include.

Some of the categories in the Shared cM Chart from DNAPainter, above, don’t work based on ages, so I’ve eliminated those. I also know, for example, that someone who could fall in the grandparent/grandchild category (blue star,) in my case, does not, so must be a different relationship.

Second cousins, who share great-grandparents, can be expected to share about 229 cM of DNA on average, or between 41 and 592 cM. First cousins share 866 cM, and half first cousins share 449 cM on average.

I have 13 close matches (over 200 cM), but I’m including my top 15 at each vendor, so I added two more. You can always go back and add more matches if necessary. Just keep in mind that the smaller the match, the greater the probability that it came from increasingly distant generations before your grandparents. Your sweet spot to identify grandparents is between 1C and 2C.

I need to divide my close matches into 4 groups, each one equating to a grandparent. Record this on your spreadsheet.

You can group your matches at Ancestry using colored dots, which means you can sort by those groups.

You can also select a “side” for a match by clicking on “Yes” under the question, “Do you recognize them?”

Initially, you want to determine if this person is related to you on your mother’s or father side, and hopefully, through which grandparent.

Recently, Ancestry added a feature called SideView which allows testers to indicate, based on ethnicity, which side is “parent 1” and which side is “parent 2.” I wrote about that, here.

Make your selection, assuming you can tell which “side” of you descends from which parent based on ethnicity and/or shared matches. How you label “parent 1,” meaning either maternal or paternal, determines how Ancestry assigns your matches, when possible.

Using these tools, which may not be completely accurate, plus shared matches with people you can identify, divide your matches among your three known grandparents, meaning that the people you cannot assign will be placed in the fourth “unknown” column.

On my spreadsheet, I assign all of my closest matches to one of my grandparents. Michael is my first cousin (1C) and we share both maternal grandparents, so he’s not helpful in the division because he can’t be assigned to only one grandparent.

The green maternal grandfather is who I’m attempting to identify.

There are 4 people, highlighted in yellow, who don’t fall into the other three grandparent lines, so they get added to the green column and will be my focus.

I would be inclined to continue adding matches using a process known as the Leeds Method, until I had several people in each category. Looking back at the DNAPainter cM chart, at this point, we don’t have anyone below 200 cM and the matches we need might be below that threshold. The more matches you have to work with, the better.

At Ancestry, you cannot download your matches into a spreadsheet, nor can you work with other clustering tools such as Genetic Affairs, so you’ll have to build out your spreadsheet manually.

Check for the same types of information that I reviewed at 23andMe:

  • Review trees, if your matches have them, minimally recording the surnames of their 8 great-grandparents.
  • Review shared matches, looking for common names in the trees in recent generations.
  • View shared matches with people with whom you have a “Common Ancestor” indication, which means a ThruLine. You won’t have Thrulines with your target grandparent, of course, but Thrulines will allow you to place the match in one of the other columns. I wrote about ThruLines here, here and here.
  • ThruLines sometimes suggests ancestors based on other people’s trees, so be EXCEEDINGLY careful with potential ancestor suggestions. That’s not to say you should discount those suggestions. Just treat them as tree hints that may have been copy/pasted hundreds of times, because that’s what they are.

I make notes on each match so I can easily see the connection by scanning without opening the match.

Now, I have a total of 30 entries on my spreadsheet, 15 from 23and Me and 15 from Ancestry.

Why Not Use Autosclusters?

Even with vendors who allow or provide cluster tools, I don’t use an automated autocluster tool at this point. Autocluster tools often omit your closest matches because your closest matches would be in nearly half of all your clusters, which isn’t exactly informative. However, for this purpose, those are the very matches we need to evaluate.

After identifying groups of people that represent the missing grandparent, using our spreadsheet methodology, autoclusters could be useful to identify common surnames and even to compare the trees of our matches using AutoTree, AutoPedigree and AutoKinship. AutoClusters cannot be utilized at Ancestry, but is available through MyHeritage and at GEDmatch, or through Genetic Affairs for 23andMe and FamilyTreeDNA.

Next, let’s move to FamilyTreeDNA.

FamilyTreeDNA

FamilyTreeDNA is the only vendor that provides Family Matching, also known as “bucketing.” FamilyTreeDNA assigns your matches to either a paternal or maternal bucket, or both, based on triangulated matches with someone you’ve linked to a profile in your tree.

The key to Family Matching is to link known Family Finder matches to their profile cards in your tree.

Clicking on the Family Tree link at the top of your personal page allows you to link your matches to the profile cards of your matches.

FamilyTreeDNA utilizes these linked matches to assign those people, and matches who match you and those people, both, on at least one common segment, to the maternal or paternal tabs on your match list.

Always link as many known people as possible (red stars) which will result in more matches being bucketed and assigned to parents’ sides for you, even if neither parent is available to test.

I wrote about Triangulation in Action at FamilyTreeDNA, here.

You can see at the top of my match list that I have a total of 8000 matches of which 3422 are paternal, 1517 are maternal and 3 match on both sides. Full siblings, their (and my) children and their descendants will always match on both sides. People with endogamy across both parents may have several matches on both sides.

If your relevant parent has tested, always work from their test.

Because we are searching for the maternal grandfather, in this case, we can ignore all tests that are bucketed as paternal matches.

Given that we are searching for my maternal grandfather, I probably have not been able to link as many maternal matches, other than possibly ones from my maternal grandmother. This means that the maternal grandfather’s matches are not bucketed because there are no identified matches to link on that side of my tree.

If you sort by maternal and paternal tabs, you’ll miss people who aren’t bucketed, meaning they have no maternal or paternal icon, so I recommend simply scanning down the list and processing maternal matches and non-bucketed matches.

By being able to confidently ignore paternally bucketed matches and only processing maternal and non-assigned matches, this is equivalent to processing the first 48 total matches. If I were to only look at the first 15 matches, 12 were paternal and only 3 are maternal.

Using bucketing at FamilyTreeDNA is very efficient and saves a lot of work.

Omitting paternal matches also means we are including smaller matches which could potentially be from common ancestors further back in the tree. Or, they could be younger testers. Or simply smaller by the randomness of recombination.

FamilyTreeDNA is a goldmine, with 16 of 20 maternal matches being from the unknown maternal grandfather.

Next, let’s see what’s waiting at MyHeritage.

MyHeritage

MyHeritage is particularly useful if your lineage happens to be from Europe. Of course, if you’re searching for an unknown person, you probably have no idea where they or their ancestors are from. Two of my best matches first appeared at MyHeritage.

Of course, your matches with people who descend from your unknown maternal grandfather won’t have any Theories of Family Relativity, as that tool is based on BOTH a DNA match plus a tree or document match. However, Theories is wonderful to group your matches to your other three grandparents.

MyHeritage provides a great deal of information for each match, including common surnames with your tree. If you recognize the surnames (and shared matches) as paternal or maternal, then you can assign the match. However, the matches you’re most interested in are the highest matches without any surnames in common with you – which likely point to the missing maternal grandfather.

However, those people may, and probably do, have surnames in common with each other.

Of the matches who aren’t attributed to the other three grandparents, the name Ferverda arises again and again. So does Miller, which suggests the grandparent or great-grandparent couple may well be Ferverda/Miller.

Let’s continue working through the process with our spreadsheet and see what we can discover about those surnames.

Our 60 Results

Of the 60 total results, 15 from each vendor, a total of 24 cannot be assigned to other columns through bucketing or shared matches, so are associated with the maternal grandfather. Of course, Michael who descends from both of my maternal grandparents won’t be helpful initially.

Cheryl, Donald and Michael are duplicates at different vendors, but the rest are not.

Of the relevant matches, the majority, 12 are from FamilyTreeDNA, four each are from Ancestry and MyHeritage, and three are from 23andMe.

Of the names provided in the surname fields of matches, in matches’ trees in the first few generations, and the testers’ surnames, Ferverda is repeated 12 times, for 50% of the time. Miller is repeated 9 times, so it’s likely that either of those are the missing grandfather’s surname. Of course, if we had Y-DNA, we’d know the answer to that immediately.

Comparing trees of my matches, we find John Ferverda as the common ancestor between two different matches. John is the son of Hiram Ferverda and Eva Miller who are found in several trees.

That’s a great hint. But is this the breakthrough I need?

What’s Next?

The next step is to look for connections between the maternal grandmother, Edith Lore, who is known in our example, and a Ferverda male. He is probably one of the sons of Hiram Ferverda and Eva Miller. Do they lived in the same area? In close proximity? Do they attend the same church or school? Are they neighbors or live close to the family or some of their relatives? Does she have connections with Ferverda family members? We are narrowing in.

Some of Hiram and Eva’s sons might be able to be eliminated based on age or other factors, or at least be less likely candidates. Any of their children who had moved out of state when the child was conceived would be less likely candidates. Age would be a factor, as would opportunity.

Target testing of the Ferverda sons’ children, or the descendants of their children would (probably) be able to pinpoint which of their sons is more closely related to me (or my mother) than the rest.

In our case, indeed, John Ferverda is the son we are searching for and his descendant, Michael is the highest match on the list. Cheryl and Donald descend from John’s brother, which eliminates him as a candidate. Another tester descends from a third Ferverda son, which eliminates that son as well.

Michael, my actual first cousin with a 755 cM match at one vendor, and 822 cM at a second vendor, is shown by the MyHeritage cM Explainer with an 88% probability that he is my first cousin.

However, when I’m trying to identify the maternal grandfather, which is half of that couple, I need to focus one generation further back in time to eliminate other candidates.

The second and third closest matches are both Donald at 395 cM and Cheryl at 467 cM who also share the same Ferverda/Miller lineage and are the children of my maternal grandfather’s brother.

On the spreadsheet, I need to look at the trees of people who have both Ferverda and Miller, which brought me to both Cheryl and Donald, then Michael, which allowed me to identify John Ferverda, unquestionably, as my grandfather based on the cM match amounts.

Cheryl and Donald, who are confirmed full siblings, and my mother either have to be first cousins, or half siblings. Their match with mother is NOT in the half-sibling range for one sibling, and on the lower edge with the other. Mother also matches Michael as a nephew, not more distantly as she would if he were a first cousin once removed (1C1R) instead of a nephew.

Evaluating these matches combined confirms that my maternal grandfather is indeed John Ferverda.

What About X DNA?

The X chromosome has a unique inheritance path which is sometimes helpful in this circumstance, especially to males.

Women inherit an X chromosome from both parents, but males inherit an X chromosome from ONLY their mother. A male inherits a Y chromosome from his father which is what makes him male. Women inherit two X chromosomes, one from each parent, and no Y, which is what makes them female.

Therefore, if you are a male and are struggling with which side of your tree matches are associated with, the X chromosome may be of help.

Your mother passed her X chromosome to you, which could be:

  • Her entire maternal X, meaning your maternal grandmother’s X chromosome
  • Her entire paternal X, meaning your maternal grandfather’s X chromosome (which descends from his mother)
  • Some combination of your maternal grandmother and maternal grandfather’s chromosomes

One thing we know positively is that a male’s X matches are ALWAYS from their maternal side only, so that should help when dividing a male’s matches maternally or paternally. Note – be aware of potential pedigree collapse, endogamy and identical-by-chance matches if it looks like a male has a X match on his father’s side.

Unfortunately, the X chromosome cannot assist females in the same way, because females inherit an X from both parents. Therefore, they can match people in the same was as a male, but also in additional ways.

  • Females will match their paternal grandmother on her entire X chromosome, and will match one or both of their maternal grandparents on the X chromosome.
  • Females will NEVER match their paternal grandfather’s X chromosome because their father did not inherit an X chromosome from his father.
  • Males will match one or both of their maternal grandparents on their X chromosome.
  • Males will NEVER match their paternal grandparents, because males do not receive an X chromosome from their father.

The usefulness of X DNA matching depends on the inheritance path of both the tester AND their match.

When Can Y-DNA or Mitochondrial DNA Help with Grandparent Identification?

If you recall, I selected the maternal grandfather as the person to seek because no tester carries either the Y-DNA or mitochondrial DNA of their maternal grandfather. In other words, this was the most difficult identification, meaning that any of the other three grandparents would be, or at least could be, easier with the benefit of Y-DNA and/or mitochondrial DNA testing.

In addition to matching, both Y-DNA and mitochondrial DNA will provide testers with location origins, both continental and often much more specific locations based on where other testers and matches are from.

Y-DNA often provides a surname.

Let’s see how these tests, matches and results can assist us.

  • Paternal grandfather – If I was a male descended from John Ferverda paternally, I could have tested both my autosomal DNA PLUS my Y-DNA, which would have immediately revealed the Ferverda surname via Y-DNA. Two Ferverda men are shown in the Ferverda surname DNA project, above.

That revelation would have confirmed the Ferverda surname when combined with the high frequency of Ferverda found among autosomal matches on the spreadsheet.

  • Maternal grandmother – If we were searching for a maternal grandmother, both the male and female sibling testers (as shown in the pedigree chart) would have her mitochondrial DNA which could provide matches to relevant descendants. Mitochondrial DNA at both FamilyTreeDNA and 23andMe could also eliminate anyone who does not match on a common haplogroup, when comparing 23andMe results to 23andMe results, and FamilyTreeDNA to FamilyTreeDNA results at the same level.

At 23andMe, only base level haplogroups are provided, but they are enough to rule out a direct matrilineal line ancestor.

At FamilyTreeDNA, the earlier HVR1 and HVR2 tests provide base level haplogroups, while full sequence testing provides granular, specific haplogroups. Full sequence is the recommended testing level.

  • Paternal grandmother – If we were searching for a paternal grandmother, testers would, of course, need either their father to test his mitochondrial DNA, or for one of his siblings to test which could be used in the same way as described for maternal grandmother matching.

Summary

Successfully identifying a grandparent is dependent on many factors. Before you make that identification, it’s very difficult to know which are more or less important.

For example, if the grandparent is from a part of the world with few testers, you will have far fewer matches, potentially, than other lines from more highly tested regions. In my case, two of my four grandparents’ families, including Ferverda, immigrated in the 1850s, so they had fewer matches than families that have been producing large families in the US for generations.

Endogamy may be a factor.

Family size in past and current generations may be a factor.

Simple luck may be a factor.

Therefore, it’s always wise to test your DNA, and that of your parents and close relatives if possible, and upload to all of the autosomal databases. Then construct an analysis plan based on:

  • How you descend from the grandparent in question, meaning do you carry their X DNA, Y-DNA or mitochondrial DNA.
  • Who else is available to test their autosomal DNA to assist with shared matches and the process of elimination.
  • Who else is available to test for Y-DNA and/or mitochondrial DNA of the ancestor in question.

If you don’t find the answer initially, schedule a revisit of your matches periodically and update your spreadsheet. Sometimes DNA and genealogy is a waiting same.

Just remember, luck always favors the prepared!

Resources

You may find the following resource articles beneficial in addition to the links provided throughout this article.

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Thank you so much.

DNA Purchases and Free Uploads

Genealogy Products and Services

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DNA Black Friday is Here

Yes, I know it’s not Friday yet, but the DNA Black Friday sales have started, and sale dates are limited, so here we go.

These are the best prices I’ve ever seen at both FamilyTreeDNA and MyHeritage. If you’ve been waiting to purchase a DNA test for that special someone, there’s never been a better time.

Remember, to jump-start your genetic genealogy, test close or targeted relatives in addition to yourself:

  • Parents, or if both parents are not available, full and half-siblings
  • If neither parents nor siblings are available, your siblings’ descendants
  • Grandparents or descendants of your grandparents – aunts, uncles, or their descendants
  • Cousins descended from great-grandparents or other known ancestors
  • Y and mitochondrial DNA descendants of specific, targeted ancestors

For yourself, you’ll want to fish in all the ponds by taking an autosomal test or uploading a DNA file to each of the four vendors. Upload/download instructions are available here.

Everyone can test their own mitochondrial DNA to learn about your mother’s direct matrilineal line, and males can test their Y-DNA to unveil information about their patrilineal or surname line. Women, you can test your father’s, brother’s, or paternal uncle’s Y-DNA.

I’ve written a DNA explainer article, 4 Kinds of DNA for Genetic Genealogy, which you might find helpful. Please feel free to pass it on.

Vendor Offerings

FamilyTreeDNA

Free shipping within the US for orders of $79 or more

FamilyTreeDNA is the only major testing company that offers multiple types of tests, meaning Y-DNA, mitochondrial and autosomal. You can also get your toes wet with introductory level tests for Y DNA (37 and 111 marker tests), or you can go for the big gun right away with the Big Y-700.

This means that if you’ve purchased tests in the past, you can upgrade now. Upgrade pricing is shown below. Click here to sign on to your account to purchase an upgrade or additional product.

At FamilyTreeDNA, by taking advantage of autosomal plus Y-DNA and mitochondrial DNA, you will get to know your ancestors in ways not possible elsewhere. You can even identify or track them using your myOrigins painted ethnicity segments.

FamilyTreeDNA divides your Family Finder matches maternal and paternally for you if you create or upload a tree and link known testers. How cool is this?!!!

MyHeritage

The MyHeritage DNA test is on sale for $36, the best autosomal test price I’ve ever seen anyplace.

MyHeritage has a significant European presence and I find European matches at MyHeritage that aren’t anyplace else. MyHeritage utilizes user trees and DNA matches to construct Theories of Family Relativity that shows how you and your matches may be related.

Remember, you can upload the raw data file from the MyHeritage DNA test to both FamilyTreeDNA and GEDmatch for free.

Free shipping on 2 kits or more.

This sale ends at the end-of-day on Black Friday.

You can combine your DNA test with a MyHeritage records subscription with a free trial, here.

Ancestry

The AncestryDNA test is $59, here. With Ancestry’s super-size DNA database, you’re sure to get lots of matches and hints via ThruLines.

You can get free shipping if you’re an Amazon Prime member.

If you order an AncestryDNA test, you can upload the raw DNA file to FamilyTreeDNA, MyHeritage and GEDmatch for free. Unfortunately, Ancestry does not accept uploads from other vendors.

23andMe

The 23andMe Ancestry + Traits DNA test is $79, here. 23andMe is well known for its Ancestry Composition (ethnicity) results and one-of-a-kind genetic tree.

The 23andMe Ancestry + Traits + Health test is now $99, here.

You can get free shipping if you’re an Amazon Prime member.

If you order either of the 23andMe tests, you can upload the raw data file to FamilyTreeDNA, MyHeritage, and GEDmatch for free. Unfortunately, 23andMe does not accept uploads from other vendors.

Can’t Wait!!

This is always my favorite time of the year because I know that beginning soon, we will all be receiving lots of new matches from people who purchased or received DNA tests during the holiday season.

  • What can you do to enhance your genealogy?
  • Have you ordered Y and mitochondrial DNA tests for yourself and people who carry the Y and mitochondrial DNA of your ancestors?
  • Are you in all of the autosomal databases?
  • Who are you ordering tests for?

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Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an email whenever I publish by clicking the “follow” button on the main blog page, here.

You Can Help Keep This Blog Free

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Uploads

Genealogy Products and Services

My Book

Genealogy Books

Genealogy Research

DNA: In Search of…Full and Half-Siblings

This is the fifth article in our series of articles about searching for unknown close family members, specifically; parents, grandparents, or siblings. However, these same techniques can be applied by genealogists to identify ancestors further back in time as well.

Please note that if a family member has tested and you do NOT see their results, ask them to verify that they have chosen to allow matching and for other people to view them in their match list. That process varies at different vendors.

You can also ask if they can see you in their results.

All Parties Need to Test

Searching for unknown siblings isn’t exactly searching, because to find them, they, themselves, or their descendant(s) must have taken a DNA test at the same vendor where you tested or uploaded a DNA file.

You may know through any variety of methods that they exist, or might exist, but if they don’t take a DNA test, you can’t find them using DNA. This might sound obvious, but I see people commenting and not realizing that the other sibling(s) must test too – and they may not have.

My first questions when someone comments in this vein are:

  1. Whether or not they are positive their sibling actually tested, meaning actually sent the test in to the vendor, and it was received by the testing company. You’d be surprised how many tests are living in permanent residence on someone’s countertop until it gets pushed into the drawer and forgotten about.
  2. If the person has confirmed that their sibling has results posted. They may have returned their test, but the results aren’t ready yet or there was a problem.
  3. AND that both people have authorized matching and sharing of results. Don’t hesitate to reach out to your vendor’s customer care if you need help with this.

Sibling Scenarios

The most common sibling scenarios are when one of two things happens:

  • A known sibling tests, only to discover that they don’t match you in the full sibling range, or not at all, when you expected they would
  • You discover a surprise match in the full or half-sibling range

Let’s talk about these scenarios and how to determine:

  • If someone is a sibling
  • If they are a full or half-sibling
  • If a half-sibling, if they descend from your mother or father

As with everything else genetic, we’ll be gathering and analyzing different pieces of evidence along the way.

Full and Half-Siblings

Just to make sure we are all on the same page:

  • A full sibling is someone who shares both parents with you.
  • A half-sibling is someone who shares one parent with you, but not the other parent.
  • A step-sibling is someone who shares no biological parents with you. This situation occurs when your parent marries their parent, after you are both born, and their parent becomes your step-parent. You share neither of your biological parents with a step-sibling, so you share no DNA and will not show up on each other’s match lists.
  • A three-quarters sibling is someone with whom you share one parent, but two siblings are the other parent. For example, you share the same mother, but one brother fathered you, and your father’s brother fathered your sibling. Yes, this can get very messy and is almost impossible for a non-professional to sort through, if even then. (This is not a solicitation. I do not take private clients.) We will not be addressing this situation specifically.

Caution

With any search for unknown relatives, you have no way of knowing what you will find.

In one’s mind, there are happy reunions, but you may experience something entirely different. Humans are human. Their stories are not always happy or rosy. They may have made mistakes they regret. Or they may have no regrets about anything.

Your sibling may not know about you or the situation under which you, or they, were born. Some women were victims of assault and violence, which is both humiliating and embarrassing. I wrote about difficult situations, here.

Your sibling or close family member may not be receptive to either you, your message, or even your existence. Just be prepared, because the seeking journey may not be pain-free for you or others, and may not culminate with or include happy reunions.

On the other hand, it may.

Please step back and ponder a bit about the journey you are about to undertake and the possible people that may be affected, and how. This box, once opened, cannot be closed again. Be sure you are prepared.

On the other hand, sometimes that box lid pops off, and the information simply falls in your lap one day when you open your match list, and you find yourself sitting there, in shock, staring at a match, trying to figure out what it all means.

Congratulations, You Have a Sibling!

This might not be exactly what runs through your mind when you see that you have a very close match that you weren’t expecting.

The first two things I recommend when making this sort of discovery, after a few deep breaths, a walk, and a cup of tea, are:

  • Viewing what the vendor says
  • Using the DNAPainter Shared cM Relationship Chart

Let’s start with DNAPainter.

DNAPainter

DNAPainter provides a relationship chart, here, based on the values from the Shared cM Project.

You can either enter a cM amount or a percentage of shared DNA. I prefer the cM amount, but it doesn’t really matter.

I’ll enter 2241 cM from a known half-sibling match. To enter a percent, click on the green “enter %.”

As you can see, statistically speaking, this person is slightly more likely to be a half-sibling than they are to be a full sibling. In reality, they could be either.

Looking at the chart below, DNAPainter highlights the possible relationships from the perspective of “Self.”

The average of all the self-reported relationships is shown, on top, so 2613 for a full sibling. The range is shown below, so 1613-3488 for a full sibling.

In this case, there are several possibilities for two people who share 2241 cM of DNA.

I happen to know that these two people are half-siblings, but if I didn’t, it would be impossible to tell from this information alone.

The cM range for full siblings is 1613-3488, and the cM range for half-siblings is 1160-2436.

  • The lower part of the matching range, from 1160-1613 cM is only found in half-siblings.
  • The portion of the range from 1613-2436 cM can be either half or full siblings.
  • The upper part of the range, from 2436-3488 cM is only found in full siblings.

If your results fall into the center portion of the range, you’re going to need to utilize other tools. Fortunately, we have several.

If you’ve discovered something unexpected, you’ll want to verify using these tools, regardless. Use every tool available. Ranges are not foolproof, and the upper and lower 10% of the responses were removed as outliers. You can read more about the shared cM Project, here and here.

Furthermore, people may be reporting some half-sibling relationships as full sibling relationships, because they don’t expect to be half-siblings, so the ranges may be somewhat “off.”

Relationship Probability Calculator

Third-party matching database, GEDmatch, provides a Relationship Probability Calculator tool that is based on statistical probability methods without compiled user input. Both tools are free, and while I haven’t compared every value, both seem to be reasonably accurate, although they do vary somewhat, especially at the outer ends of the ranges.

When dealing with sibling matches, if you are in all four databases, GEDmatch is a secondary resource, but I will include GEDmatch when they have a unique tool as well as in the summary table. Some of your matches may be willing to upload to GEDmatch if the vendor where you match doesn’t provide everything you need and GEDmatch has a supplemental offering.

Next, let’s look at what the vendors say about sibling matches.

Vendors

Each of the major vendors reports sibling relationships in a slightly different way.

Sibling Matches at Ancestry

Ancestry reports sibling relationships as Sister or Brother, but they don’t say half or full.

If you click on the cM portion of the link, you’ll see additional detail, below

Ancestry tells you that the possible relationships are 100% “Sibling.” The only way to discern the difference between full and half is by what’s next.

If the ONLY relationship shown is Sibling at 100%, that can be interpreted to mean this person is a full sibling, and that a half-sibling or other relationship is NOT a possibility.

Ancestry never stipulates full or half.

The following relationship is a half-sibling at Ancestry.

Ancestry identifies that possible range of relationships as “Close Family to First Cousin” because of the overlaps we saw in the DNAPainter chart.

Clicking through shows that there is a range of possible relationships, and Ancestry is 100% sure the relationship is one of those.

DNAPainter agrees with Ancestry except includes the full-sibling relationship as a possibility for 1826 cM.

Sibling Matches at 23andMe

23andMe does identify full versus half-siblings.

DNAPainter disagrees with 23andMe and claims that anyone who shares 46.2% of their DNA is a parent/child.

However, look at the fine print. 23andMe counts differently than any of the other vendors, and DNAPainter relies on the Shared cM Project, which relies on testers entering known relationship matching information. Therefore, at any other vendor, DNAPainter is probably exactly right.

Before we understand how 23andMe counts, we need to understand about half versus fully identical segments.

To determine half or full siblings, 23andMe compares two things:

  1. The amount of shared matching DNA between two people
  2. Fully Identical Regions (FIR) of DNA compared to Half Identical Regions (HIR) of DNA to determine if any of your DNA is fully identical, meaning some pieces of you and your sibling’s DNA is exactly the same on both your maternal and paternal chromosomes.

Here’s an example on any chromosome – I’ve randomly selected chromosome 12. Which chromosome doesn’t matter, except for the X, which is different.

Your match isn’t broken out by maternal and paternal sides. You would simply see, on the chromosome browser, that you and your sibling match at these locations, above.

In reality, though, you have two copies of each chromosome, one from Mom and one from Dad, and so does your sibling.

In this example, Mom’s chromosome is visualized on top, and Dad’s is on the bottom, below, but as a tester, you don’t know that. All you know is that you match your sibling on all of those blue areas, above.

However, what’s actually happening in this example is that you are matching your sibling on parts of your mother’s chromosome and parts of your father’s chromosome, shown above as green areas

23andMe looks at both copies of your chromosome, the one you inherited from Mom, on top, and Dad, on the bottom, to see if you match your sibling on BOTH your mother’s and your father’s chromosomes in that location.

I’ve boxed the green matching areas in purple where you match your sibling fully, on both parents’ chromosomes.

If you and your sibling share both parents, you will share significant amounts of the same DNA on both copies of the same chromosomes, meaning maternal and paternal. In other words, full siblings share some purple fully identical regions (FIR) of DNA with each other, while half-siblings do not (unless they are also otherwise related) because half-siblings only share one parent with each other. Their DNA can’t be fully identical because they have a different parent that contributed the other copy of their chromosome.

Total Shared DNA Fully Identical DNA from Both Parents
Full Siblings ~50% ~25%
Half Siblings ~25% 0
  • Full siblings are expected to share about 50% of the same DNA. In other words, their DNA will match at that location. That’s all the green boxed locations, above.
  • Full siblings are expected to share about 25% of the same DNA from BOTH parents at the same location on BOTH copies of their chromosomes. These are fully identical regions and are boxed in purple, above.

You’ll find fully identical segments about 25% of the time in full siblings, but you won’t find fully identical segments in half-siblings. Please note that there are exceptions for ¾ siblings and endogamous populations.

You can view each match at 23andMe to see if you have any completely identical regions, shown in dark purple in the top comparison of full siblings. Half siblings are shown in the second example, with less total matching DNA and no FIR or completely identical regions.

Please note that your matching amount of DNA will probably be higher at 23andMe than at other companies because:

  • 23andMe includes the X chromosome in the match totals
  • 23andMe counts fully identical matching regions twice. For full siblings, that’s an additional 25%

Therefore, a full sibling with an X match will have a higher total cM at 23andMe than the same siblings elsewhere because not only is the X added into the total, the FIR match region is added a second time too.

Fully Identical Regions (FIR) and Half Identical Regions (HIR) at GEDmatch

At GEDMatch, you can compare two people to each other, with an option to display the matching information and a painted graphic for each chromosome that includes FIR and HIR.

If you need to know if you and a match share fully identical regions and you haven’t tested at 23andMe, you can both upload your DNA data file to GEDmatch and use their One to One Autosomal DNA Comparison.

On the following page, simply enter both kit numbers and accept the defaults, making sure you have selected one of the graphics options.

While GEDmatch doesn’t specifically tell you whether someone is a full or half sibling, you can garner additional information about the relationship based on the graphic at GEDmatch.

GEDMatch shows both half and fully identical regions.

The above match is between two full siblings using a 7 cM threshold. The blue on the bottom bar indicates a match of 7 cM or larger. Black means no match.

The green regions in the top bar indicate places where these two people carry the same DNA on both copies of their chromosome 1. This means that both people inherited the same DNA from BOTH parents on the green segments.

In the yellow regions, the siblings inherited the same DNA from ONE parent, but different DNA in that region from the other parent. They do match each other, just on one of their chromosomes, not both.

Without a tool like this to differentiate between HIR and FIR, you can’t tell if you’re matching someone on one copy of your chromosome, or on both copies.

In the areas marked with red on top, which corresponds to the black on the bottom band, these two siblings don’t match each other because they inherited different DNA from both parents in that region. The yellow in that region is too scattered to be significant.

Full siblings generally share a significant amount of FIR, or fully identical regions of DNA – about 25%.

Half siblings will share NO significant amount of FIR, although some will be FIR on very small, scattered green segments simply by chance, as you can see in the example, below.

This half-sibling match shares no segments large enough to be a match (7 cM) in the black section. In the blue matching section, only a few small green fragments of DNA match fully, which, based on the rest of that matching segment, must be identical by chance or misreads. There are no significant contiguous segments of fully identical DNA.

When dealing with full or half-siblings, you’re not interested in small, scattered segments of fully identical regions, like those green snippets on chromosome 6, but in large contiguous sections of matching DNA like the chromosome 1 example.

GEDmatch can help when you match when a vendor does not provide FIR/HIR information, and you need additional assistance.

Next, let’s look at full and half-siblings at FamilyTreeDNA

Sibling Matches at FamilyTreeDNA

FamilyTreeDNA does identify full siblings.

Relationships other than full siblings are indicated by a range. The two individuals below are both half-sibling matches to the tester.

The full range when mousing over the relationship ranges is shown below.

DNAPainter agrees except also gives full siblings as an option for the two half-siblings.

FamilyTreeDNA also tells you if you have an X match and the size of your X match.

We will talk about X matching in a minute, which, when dealing with sibling identification, can turn out to be very important.

Sibling Matches at MyHeritage

MyHeritage indicates brother or sister for full siblings

MyHeritage provides other “Estimated relationships” for matches too small to be full siblings.

DNAPainter’s chart agrees with this classification, except adds additional relationship possibilities.

Be sure to review all of the information provided by each vendor for close relationships.

View Close Known Relationships

The next easiest step to take is to compare your full or half-sibling match to known close family members from your maternal and paternal sides, respectively. The closer the family members, the better.

It’s often not possible to determine if someone is a half sibling or a full sibling by centiMorgans (cMs) alone, especially if you’re searching for unknown family members.

Let’s start with the simplest situation first.

Let’s say both of your parents have tested, and of course, you match both of them as parents.

Your new “very close match” is in the sibling range.

The first thing to do at each vendor is to utilize that vendor’s shared matches tool and see whether your new match matches one parent, or both.

Here’s an example.

Close Relationships at FamilyTreeDNA

This person has a full sibling match, but let’s say they don’t know who this is and wants to see if their new sibling matches one or both of their parents.

Select the match by checking the box to the left of the match name, then click on the little two-person icon at far right, which shows “In Common” matches

You can see on the resulting shared match list that both of the tester’s parents are shown on the shared match list.

Now let’s make this a little more difficult.

No Parents, No Problem

Let’s say neither of your parents has tested.

If you know who your family is and can identify your matches, you can see if the sibling you match matches other close relatives on both or either side of your family.

You’ll want to view shared matches with your closest known match on both sides of your tree, beginning with the closest first. Aunts, uncles, first cousins, etc.

You will match all of your family members through second cousins, and 90% of your third cousins. You can view additional relationship percentages in the article, How Much of Them is in You?.

I recommend, for this matching purpose, to utilize 2nd cousins and closer. That way you know for sure if you don’t share them as a match with your sibling, it’s because the sibling is not related on that side of the family, not because they simply don’t share any DNA due to their distance.

In this example, you have three sibling matches. Based on your and their matches to the same known first and second cousins, you can see that:

  • Sibling 1 is your full sibling, because you both match the same maternal and paternal first and second cousins
  • Sibling 2 is your paternal half-sibling because you both match paternal second cousins and closer, but not maternal cousins.
  • Sibling 3 is your maternal half-sibling because you both match maternal second cousins and closer, but not paternal cousins.

Close Relationships at Ancestry

Neither of my parents have tested, but my first cousin on my mother’s side has. Let’s say I have a suspected sibling or half-sibling match, so I click on the match’s name, then on Shared Matches.

Sure enough, my new match also matches my first cousin that I’ve labeled as “on my mother’s side.”

If my new match in the sibling range also matches my second cousins or closer on my father’s side, the new match is a full sibling, not a half-sibling.

Close Relationships at MyHeritage

Comparing my closest match provided a real surprise. I wonder if I’ve found a half-sibling to my mother.

Now, THIS is interesting.

Hmmm. More research is needed, beginning with the age of my match. MyHeritage provides ages if the MyHeritage member authorizes that information to be shared.

Close Relationships at 23andMe

Under DNA Relatives, click on your suspected sibling match, then scroll down and select “Find Relatives in Common.”

The Relatives in Common list shows people that match both of you.

The first common match is very close and a similar relationship to my closest match on my father’s side. This would be expected of a sibling. I have no common matches with this match to anyone on my mother’s side, so they are only related on my father’s side. Therefore they are a paternal half-sibling, not a full sibling.

More Tools Are Available

Hopefully, by now, you’ve been able to determine if your mystery match is a sibling, and if so, if they are a half or full sibling, and through which parent.

We have some additional tools that are relevant and can be very informative in some circumstances. I suggest utilizing these tools, even if you think you know the answer.

In this type of situation, there’s no such thing as too much information.

X Matching

X matching, or lack thereof, may help you determine how you are related to someone.

There are two types of autosomal DNA. The X chromosome versus chromosomes 1-22. The X chromosome (number 23) has a unique inheritance path that distinguishes it from your other chromosomes.

The X chromosome inheritance path also differs between men and women.

Here’s my pedigree chart in fan form, highlighting the ancestors who may have contributed a portion of their X chromosome to me. In the closest generation, this shows that I inherited an X chromosome from both of my parents, and who in each of their lines could have contributed an X to them.

The white or uncolored positions, meaning ancestors, cannot contribute any portion of an X chromosome to me based on how the X chromosome is inherited.

You’ll notice that my father inherited none of his X chromosome from any of his paternal ancestors, so of course, I can’t inherit what he didn’t inherit. There are a very limited number of ancestors on my father’s side whom I can inherit any portion of an X chromosome from.

Men receive their Y chromosome from their fathers, so men ONLY receive an X chromosome from their mother.

Therefore, men MUST pass their mother’s X chromosome on to their female offspring because they don’t have any other copy of the X chromosome to pass on.

Men pass no X chromosome to sons.

We don’t need to worry about a full fan chart when dealing with siblings and half-siblings.

We only need to be concerned with the testers plus one generation (parents) when utilizing the X chromosome in sibling situations.

These two female Disney Princesses, above, are full siblings, and both inherited an X chromosome from BOTH their mother and father. However, their father only has one X (red) chromosome to give them, so the two females MUST match on the entire red X chromosome from their father.

Their mother has two X chromosomes, green and black, to contribute – one from each of her parents.

The full siblings, Melody, and Cinderella:

  • May have inherited some portion of the same green and black X chromosomes from their mother, so they are partial matches on their mother’s X chromosome.
  • May have inherited the exact same full X chromosome from their mother (both inherited the entire green or both inherited the entire black), so they match fully on their mother’s X chromosome.
  • May have inherited the opposite X from different maternal grandparents. One inherited the entire green X and one inherited the entire black X, so they don’t match on their mother’s X chromosome.

Now, let’s look at Cinderella, who matches Henry.

This female and male full sibling match can’t share an X chromosome on the father’s side, because the male’s father doesn’t contribute an X chromosome to him. The son, Henry, inherited a Y chromosome instead from his father, which is what made them males.

Therefore, if a male and female match on the X chromosome, it MUST be through HIS mother, but could be through either of her parents. In a sibling situation, an X match between a male and female always indicates the mother.

In the example above, the two people share both of their mother’s X chromosomes, so are definitely (at least) maternally related. They could be full siblings, but we can’t determine that by the X chromosome in this situation, with males.

However, if the male matches the female on HER father’s X chromosome, there a different message, example below.

You can see that the male is related to the female on her father’s side, where she inherited the entire magenta X chromosome. The male inherited a portion of the magenta X chromosome from his mother, so these two people do have an X match. However, he matches on his mother’s side, and she matches on her father’s side, so that’s clearly not the same parent.

  • These people CAN NOT be full siblings because they don’t match on HER mother’s side too, which would also be his mother’s side if they were full siblings.
  • They cannot be maternal half-siblings because their X DNA only matches on her father’s side, but they wouldn’t know that unless she knew which side was which based on share matches.
  • They cannot be paternal half-siblings because he does not have an X chromosome from his father.

They could, however, be uncle/aunt-niece/nephew or first cousins on his mother’s side and her father’s side. (Yes, you’re definitely going to have to read this again if you ever need male-female X matching.)

Now, let’s look at X chromosome matching between two males. It’s a lot less complicated and much more succinct.

Neither male has inherited an X chromosome from their father, so if two males DO match on the X, it MUST be through their mother. In terms of siblings, this would mean they share the same mother.

However, there is one slight twist. In the above example, you can see that the men inherited a different proportion of the green and black X chromosomes from their common mother. However, it is possible that the mother could contribute her entire green X chromosome to one son, Justin in this example, and her entire black X chromosome to Henry.

Therefore, even though Henry and Justin DO share a mother, their X chromosome would NOT match in this scenario. This is rare but does occasionally happen.

Based on the above examples, the X chromosome may be relevant in the identification of full or half siblings based on the sexes of the two people who otherwise match at a level indicating a full or half-sibling relationship.

Here’s a summary chart for sibling X matching.

X Match Female Male
Female Will match on shared father’s full X chromosome, mother’s X is the same rules as chromosomes 1-22 Match through male’s mother, but either of female’s parents. If the X match is not through the female’s mother, they are not full siblings nor maternal half-siblings. They cannot have an X match through the male’s father. They are either full or half-siblings through their mother if they match on both of their mother’s side. If they match on his mother’s side, and her father’s side, they are not siblings but could be otherwise closely related.
Male Match through male’s mother, but either of female’s parents. If the X match is not through the female’s mother, they are not full siblings nor maternal half-siblings. They cannot have an X match through the male’s father. They are either full or half-siblings through their mother if they match on both or their mother’s side. If they match on his mother’s side, and her father’s side, they are not siblings but could be otherwise closely related. Both males are related on their mother’s side – either full or half-siblings.

Here’s the information presented in a different way.

DOES match X summary:

  • If a male DOES match a female on the X, he IS related to her through HIS mother’s side, but could match her on her mother or father’s side. If their match is not through her mother, then they are not full siblings nor maternal half-siblings. They cannot match through his father, so they cannot be paternal half-siblings.
  • If a female DOES match a female on the X, they could be related on either side and could be full or half-siblings.
  • If a male DOES match a male on the X, they ARE both related through their mother. They may also be related on their father’s side, but the X does not inform us of that.

Does NOT match X summary:

  • If a male does NOT match a female on the X, they are NOT related through HIS mother and are neither full siblings nor maternal half-siblings. Since a male does not have an X chromosome from his father, they cannot be paternal half-siblings based on an X match.
  • If a male does NOT match a male, they do NOT share a mother.
  • If a female does NOT match another female on the X, they are NOT full siblings and are NOT half-siblings on their paternal side. Their father only has one X chromosome, and he would have given the same X to both daughters.

Of the four autosomal vendors, only 23andMe and FamilyTreeDNA report X chromosome results and matching, although the other two vendors, MyHeritage and Ancestry, include the X in their DNA download file so you can find X matches with those files at either FamilyTreeDNA or GEDMatch if your match has or will upload their file to either of those vendors. I wrote step-by-step detailed download/upload instructions, here.

X Matching at FamilyTreeDNA

In this example from FamilyTreeDNA, the female tester has discovered two half-sibling matches, both through her father. In the first scenario, she matches a female on the full X chromosome (181 cM). She and her half-sibling MUST share their father’s entire X chromosome because he only had one X, from his mother, to contribute to both of his daughters.

In the second match to a male half-sibling, our female tester shares NO X match because her father did not contribute an X chromosome to his son.

If we didn’t know which parents these half-sibling matches were through, we can infer from the X matching alone that the male is probably NOT through the mother.

Then by comparing shared matches with each sibling, Advanced Matches, or viewing the match Matrix, we can determine if the siblings match each other and are from the same or different sides of the family.

Under Additional Tests and Tools, Advanced Matching, FamilyTreeDNA provides an additional tool that can show only X matches combined with relationships.

Of course, you’ll need to view shared matches to see which people match the mother and/or match the father.

To see who matches each other, you’ll need to use the Matrix tool.

At FamilyTreeDNA, the Matrix, located under Autosomal DNA Results and Tools, allows you to select your matches to see if they also match each other. If you have known half-siblings, or close relatives, this is another way to view relationships.

Here’s an example using my father and two paternal half-siblings. We can see that the half-siblings also match each other, so they are (at least) half-siblings on the paternal side too.

If they also matched my mother, we would be full siblings, of course.

Next, let’s use Y DNA and mitochondrial DNA.

Y DNA and Mitochondrial DNA

In addition to autosomal DNA, we can utilize Y DNA and mitochondrial DNA (mtDNA) in some cases to identify siblings or to narrow or eliminate relationship possibilities.

Given that Y DNA and mitochondrial DNA both have distinctive inheritance paths, full and half-siblings will, or will not, match under various circumstances.

Y DNA

Y DNA is passed intact from father to son, meaning it’s not admixed with any of the mother’s DNA. Daughters do not inherit Y DNA from their father, so Y DNA is only useful for male-to-male comparisons.

Two types of Y DNA are used for genealogy, STR markers for matching, and haplogroups, and both are equally powerful in slightly different ways.

Y DNA at FamilyTreeDNA

Men can order either 37 or 111 STR marker tests, or the BIg Y which provides more than 700 markers and more. FamilyTreeDNA is the only one of the vendors to offer Y DNA testing that includes STR markers and matching between men.

Men who order these tests will be compared for matching on either 37, 111 or 700 STR markers in addition to SNP markers used for haplogroup identification and assignment.

Fathers will certainly match their sons, and paternal line brothers will match each other, but they will also match people more distantly related.

However, if two men are NOT either full or half siblings on the paternal side, they won’t match at 111 markers.

If two men DON’T match, especially at high marker levels, they likely aren’t siblings. The word “likely” is in there because, very occasionally, a large deletion occurs that prevents STR matching, especially at lower levels.

Additionally, men who take the 37 or 111 marker test also receive an estimated haplogroup at a high level for free, without any additional testing.

However, if men take the Big Y-700 test, they not only will (or won’t) match on up to 700 STR markers, they will also receive a VERY refined haplogroup via SNP marker testing that is often even more sensitive in terms of matching than STR markers. Between these two types of markers, Y DNA testing can place men very granularly in relation to other men.

Men can match in two ways on Y DNA, and the results are very enlightening.

If two men match on BOTH their most refined haplogroup (Big Y test) AND STR markers, they could certainly be siblings or father/son. They could also be related on the same line for another reason, such as known or unknown cousins or closer relationships like uncle/nephew. Of course, Y DNA, in addition to autosomal matching, is a powerful combination.

Conversely, if two men don’t have a similar or close haplogroup, they are not a father and son or paternal line siblings.

FamilyTreeDNA offers both inexpensive entry-level testing (37 and 111 markers) and highly refined advanced testing of most of the Y chromosome (Big Y-700), so haplogroup assignments can vary widely based on the test you take. This makes haplogroup matching and interpretation a bit more complex.

For example, haplogroups R-M269 and I-BY14000 are not related in thousands of years. One is haplogroup R, and one is haplogroup I – completely different branches of the Y DNA tree. These two men won’t match on STR markers or their haplogroup.

However, because FamilyTreeDNA provides over 50,000 different haplogroups, or tree branches, for Big Y testers, and they provide VERY granular matching, two father/son or sibling males who have BOTH tested at the Big Y-700 level will have either the exact same haplogroup, or at most, one branch difference on the tree if a mutation occurred between father and son.

If both men have NOT tested at the Big Y-700 level, their haplogroups will be on the same branch. For example, a man who has only taken a 37/111 marker STR test may be estimated at R-M269, which is certainly accurate as far as it goes.

His sibling who has taken a Big Y test will be many branches further downstream on the tree – but on the same large haplogroup R-M269 branch. It’s essential to pay attention to which tests a Y DNA match has taken when analyzing the match.

The beauty of the two kinds of tests is that even if one haplogroup is very general due to no Big Y test, their STR markers should still match. It’s just that sometimes this means that one hand is tied behind your back.

Y DNA matching alone can eliminate the possibility of a direct paternal line connection, but it cannot prove siblingship or paternity alone – not without additional information.

The Advanced Matching tool will provide a list of matches in all categories selected – in this case, both the 111 markers and the Family Finder test. You can see that one of these men is the father of the tester, and one is the full sibling.

You can view haplogroup assignments on the public Y DNA tree, here. I wrote about using the public tree, here.

In addition, recently, FamilyTreeDNA launched the new Y DNA Discover tool, which explains more about haplogroups, including their ages and other fun facts like migration paths along with notable and ancient connections. I wrote about using the Discover tool, here.

Y DNA at 23andMe

Testers receive a base haplogroup with their autosomal test. 23andMe tests a limited number of Y DNA SNP locations, but they don’t test many, and they don’t test STR markers, so there is no Y DNA matching and no refined haplogroups.

You can view the haplogroups of your matches. If your male sibling match does NOT share the same haplogroup, the two men are not paternal line siblings. If two men DO share the same haplogroup, they MIGHT be paternal siblings. They also might not.

Again, autosomal close matching plus haplogroup comparisons include or exclude paternal side siblings for males.

Paternal side siblings at 23andMe share the same haplogroup, but so do many other people. These two men could be siblings. The haplogroups don’t exclude that possibility. If the haplogroups were different, that would exclude being either full or paternal half-siblings.

Men can also compare their mitochondrial DNA to eliminate a maternal relationship.

These men are not full siblings or maternal half-siblings. We know, unquestionably, because their mitochondrial haplogroups don’t match.

23andMe also constructs a genetic tree, but often struggles with close relative placement, especially when half-relationships are involved. I do not recommend relying on the genetic tree in this circumstance.

Mitochondrial DNA

Mitochondrial DNA is passed from mothers to all of their children, but only females pass it on. If two people, males or females, don’t match on their mitochondrial DNA test, with a couple of possible exceptions, they are NOT full siblings, and they are NOT maternal half-siblings.

Mitochondrial DNA at 23andMe

23andMe provides limited, base mitochondrial haplogroups, but no matching. If two people don’t have the same haplogroup at 23andMe, they aren’t full or maternal siblings, as illustrated above.

Mitochondrial DNA at FamilyTreeDNA

FamilyTreeDNA provides both mitochondrial matching AND a much more refined haplogroup. The full sequence test (mtFull), the only version sold today, is essential for reliable comparisons.

Full siblings or maternal half-siblings will always share the same haplogroup, regardless of their sex.

Generally, a full sibling or maternal half-sibling match will match exactly at the full mitochondrial sequence (FMS) level with a genetic distance of zero, meaning fully matching and no mismatching mutations.

There are rare instances where maternal siblings or even mothers and children do not match exactly, meaning they have a genetic distance of greater than 0, because of a mutation called a heteroplasmy.

I wrote about heteroplasmies, here.

Like Y DNA, mitochondrial DNA cannot identify a sibling or parental relationship without additional evidence, but it can exclude one, and it can also provide much-needed evidence in conjunction with autosomal matching. The great news is that unlike Y DNA, everyone has mitochondrial DNA and it comes directly from their mother.

Once again, FamilyTreeDNA’s Advanced Matching tool provides a list of people who match you on both your mitochondrial DNA test and the Family Finder autosomal test, including transfers/uploads, and provides a relationship.

You can see that our tester matches both a full sibling and their mother. Of course, a parent/child match could mean that our tester is a female and one of her children, of either sex, has tested.

Below is an example of a parent-child match that has experienced a heteroplasmy.

Based on the comparison of both the mitochondrial DNA test, plus the autosomal Family Finder test, you can verify that this is a close family relationship.

You can also eliminate potential relationships based on the mitochondrial DNA inheritance path. The mitochondrial DNA of full siblings and maternal half-siblings will always match at the full sequence and haplogroup level, and paternal half-siblings will never match. If paternal half-siblings do match, it’s happenstance or because of a different reason.

Sibling Summary and Checklist

I’ve created a quick reference checklist for you to use when attempting to determine whether or not a match is a sibling, and, if so, whether they are half or full siblings. Of course, these tools are in addition to the DNAPainter Shared cM Tool and GEDmatch’s Relationship Predictor Calculator.

FamilyTreeDNA Ancestry 23andMe MyHeritage GEDmatch
Matching Yes Yes Yes Yes Yes
Shared Matches Yes – In Common With Yes – Shared Matches Yes – Relatives in Common Yes – Review DNA Match Yes – People who match both or 1 of 2 kits
Relationship Between Shared Matches No No No Yes, under shared match No
Matches Match Each Other* Yes, Matrix No Yes, under “View DNA details,” then, “compare with more relatives” Partly, through triangulation Yes, can match any kits
Full Siblings Yes Sibling, implies full Yes Brother, Sister, means full No
Half Siblings Sibling, Uncle/Aunt-Niece/Nephew, Grandparent-Grandchild Close Family – 1C Yes Half sibling, aunt/uncle-niece-nephew No
Fully Identical Regions (FIR) No No Yes No Yes
Half Identical Regions (HIR) No No Yes No Yes
X matching Yes No Yes No Yes
Unusual Reporting or Anomalies No No, Timber is not used on close relationships X match added into total, FIR added twice No Matching amount can vary from vendors
Y DNA Yes, STRs, refined haplogroups, matching No High-level haplogroup only, no matching No No, only if tester enters haplogroup manually
Mitochondrial DNA Yes, full sequence, matching, refined haplogroup No High-level haplogroup only, no matching No No, only if tester enters haplogroup manually
Combined Tools (Autosomal, X, Y, mtDNA) Yes No No No No

*Autoclusters through Genetic Affairs show cluster relationships of matches to the tester and to each other, but not all matches are included, including close matches. While this is a great tool, it’s not relevant for determining close and sibling relationships. See the article, AutoClustering by Genetic Affairs, here.

Additional Resources

Some of you may be wondering how endogamy affects sibling numbers.

Endogamy makes almost everything a little more complex. I wrote about endogamy and various ways to determine if you have an endogamous heritage, here.

Please note that half-siblings with high cM matches also fall into the range of full siblings (1613-3488), with or without endogamy. This may be, but is not always, especially pronounced in endogamous groups.

As another resource, I wrote an earlier article, Full or Half Siblings, here, that includes some different examples.

Strategy

You have a lot of quills in your quiver now, and I wish you the best if you’re trying to unravel a siblingship mystery.

You may not know who your biological family is, or maybe your sibling doesn’t know who their family is, but perhaps your close relatives know who their family is and can help. Remember, the situation that has revealed itself may be a shock to everyone involved.

Above all, be kind and take things slow. If your unexpected sibling match becomes frightened or overwhelmed, they may simply check out and either delete their DNA results altogether or block you. They may have that reaction before you have a chance to do anything.

Because of that possibility, I recommend performing your analysis quickly, along with taking relevant screenshots before reaching out so you will at least have that much information to work with, just in case things go belly up.

When you’re ready to make contact, I suggest beginning by sending a friendly, short, message saying that you’ve noticed that you have a close match (don’t say sibling) and asking what they know about their family genealogy – maybe ask who their grandparents are or if they have family living in the area where you live. I recommend including a little bit of information about yourself, such as where you were born and are from.

I also refrain from using the word adoption (or similar) in the beginning or giving too much detailed information, because it sometimes frightens people, especially if they know or discover that there’s a painful or embarrassing family situation.

And, please, never, ever assume the worst of anyone or their motives. They may be sitting at their keyboard with the same shocked look on their face as you – especially if they have, or had, no idea. They may need space and time to reach a place of acceptance. There’s just nothing more emotionally boat-capsizing in your life than discovering intimate and personal details about your parents, one or both, especially if that discovery is disappointing and image-altering.

Or, conversely, your sibling may have been hoping and waiting just for you!

Take a deep breath and let me know how it goes!

Please feel free to share this article with anyone who could benefit.

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DNA: In Search of… New Series Launches

Today, I’m excited to announce a new series titled “DNA: In Search of…”

I receive queries every single day about how to search for either unknown parents or unknown grandparents using genetic genealogy.

While some of the techniques are the same when searching for these different people, others vary and depend on a combination of factors: Continue reading

Million Mito Project Team – Introduction and Progress Update

Let me introduce you to the Million Mito Project team.

Left to right, Goran Runfeldt, Dr. Paul Maier, me, and Dr. Miguel Vilar. And yes, I know we look kind of like a band😊. The Merry Mito Band maybe, except, trust me, I can’t sing.

Yes, we finally, finally got to meet in person recently, and let me tell you, that was one joyful meeting. I hadn’t realized that while I know everyone, not everyone else had met in person before.

We have been working for almost two years together via Zoom, but separately. Just 10 days after the Million Mito Project was announced, we went into Covid lockdown.

It’s difficult to work remotely on such a huge collaborative project, but we have been making inroads, albeit slower than we had initially hoped.

Complicating this was the merger of FamilyTreeDNA with myDNA in January of 2021, with Bennett Greenspan stepping down as the CEO in that process. Bennett greenlit the Million Mito project initially. (Thank you, Bennett!)

Thankfully, the new CEO, Dr. Lior Rauschberger continued that greenlight without hesitation as soon our team was able to inform him about this wonderful scientific project that was underway. (Thank you, Lior!)

I can’t tell you what a HUGE relief that was.

While all change is challenging, and complicated by the Covid landscape, life events, and geographic distance, that merger really was the right decision. Lior is committed to scientific research, discovery, and the genealogy marketspace. He’s looking to expand, not contract.

You’re probably wondering where we are now in the Million Mito process.

Million Mito Project Update

I’d like to provide a brief update.

  • We have an academic paper in the final stages of the submission process, but this paper is not the final tree. It is, however, something extremely cool and important to the history of womankind! I can’t say more until publication, but I’ll write an article when the paper is published.
  • The team hopes to work with a million samples between all sources including FamilyTreeDNA testers, research-consented Genographic samples, Genbank, and other academic samples. Not all samples from those sources are full mitochondrial sequences, or necessarily pass our QC checks.

If you haven’t yet taken a full sequence test, you can help reach the one million goal by ordering a mitochondrial DNA test at FamilyTreeDNA, here. If you tested at a lower level some years back, please sign on to your account and upgrade so you can be a part of this scientific frontier.

  • We discovered that the authors of Phylotree never documented the “recipe” for reconstructing the tree behind the scenes, so we can’t exactly use the recipe for Phylotree as the basis for constructing a future tree.
  • We have been in the process of writing phylogenetic software that arrives at a similar tree to use as a baseline reference structure in order to preserve as many of the current Phylotree haplogroup names as possible.

Hand curation and placement is possible for hundreds or a few thousand samples, but it’s not possible for large numbers. While phylogenetic software to do this kind of work has existed for a long time, it typically can’t handle huge trees like what we are building.

Phylogenetic methods also struggle with highly recurrent mutations, and rapid star-burst expansions that we see on the human trees. A phylogenetic problem of this magnitude requires lots of innovations to correctly interpret lineage history from complex mutations.

Automated software to handle very large numbers of sequences must be adapted or developed.

  • Furthermore, simply building upon an existing scaffold without automating the process does not provide an ongoing, sustainable procedure to discover where new dividing branches are discovered internally within the tree, versus at the tips. In other words, adding new branches based on common mutations is only easy when you’re simply appending a new haplogroup to an existing one.

For example, I might have a new haplogroup J1c2f1 derived from J1c2f. That’s easy. It’s another matter entirely if haplogroup J1 itself, high up in the tree, were broken into multiple new branches. Only automated software can “reconstruct” the tree regularly to discover new major branches as the results of more testers become available.

Challenges

Let me share some examples of the kinds of challenges that we’ve encountered. Not only are these interesting, but they are also educational.

These figures are from Paul Maier’s RootsTech presentation, which I strongly recommend that you view, here.

Mitochondrial DNA is both fascinating and habit-forming. The more you know, the more you want to know.

Let’s start with the basics. Haplogroups are defined by one or more mutations that everyone upstream does NOT have, and everyone downstream DOES have.

Pretty simple so far, right!

Haplogroup-Defining Mutations

Here’s an example of a nice simple mutation that is one of the multiple mutations that define haplogroup L1, near the base of the mitochondrial tree (Mitochondrial Eve) in the center. At location 3666, the “normal” value is G, but in this branch, the G in that position has been replaced by an A.

You can see that the other haplogroups shown in the circle by black dots don’t have the G-to-A mutation at location 3666, but the red dot locations do carry that mutation. Therefore, G3666A is one of the mutations that defines haplogroup L1. Haplogroups can be defined by only one unique mutation, or multiple mutations.

Multiple Haplogroup-Defining Mutations

Haplogroups with multiple mutations that define that specific haplogroup are candidates to be split into multiple branches forming new haplogroups at some point in the future when other people test who have:

  1. One or the other of those mutations if there are only two
  2. A subset of the mutations
  3. But not all of the mutations

Click on images to enlarge

For example, in the view of the public mitochondrial haplotree at FamilyTreeDNA which you can view here, you see that haplogroup L1 is defined by a total of 6 mutations. Someday, people may test that only have half (or a portion) of those mutations which would cause haplogroup L1 to split or branch into two separate haplogroups.

Unstable Mutations

Some mitochondrial locations are unstable, such as 16519C, along with a few other hypervariable locations. By unstable, I mean that they have mutated back and forth in the tree many times. The historical branching patterns of such unstable mutations can be difficult to decipher (the technical term is “saturation”), suggesting perhaps that they should not be the foundation for a new haplogroup.

Do we ignore those unstable locations entirely?

After discounting those well-known unstable locations, we still find some mutations, often in the HVR (hypervariable) regions that occur close to 100 times in the full tree.

This mutation at location 150 from C to T occurred four distinct times just in this small subset of haplogroup L. You can see the 4 locations I’ve bracketed with red boxes.

Is C150T stable enough to form a haplogroup? Multiple haplogroups? Should it be used high in the tree if this affects the complete downstream structure?

This same mutation occurs additional times further downstream in the tree, as well.

Reverse Mutations

Of course, some haplogroups are defined by reverse mutations, where the original mutation reverts back to its original state.

What about locations that have as many as 3 reverse mutations, which means that one location mutates back and forth 6 times in total? Kind of like a drunken sailor zigging and zagging along the street.

If we counted each mutation and reversal as a new haplogroup, we would have 6 new haplogroups based on this one single location in one parent haplogroup. Is that accurate, or should we ignore it altogether?

Here’s an example of one mutation and a corresponding back mutation.

In this scenario, the mutation of location 7055 from A to G occurred once in the formation of haplogroup L1. However, a back mutation took place, signified by the ! (exclamation mark) after the A, which is a defining mutation for haplogroup L1c3. All of the other L1c haplogroups still carry the A to G mutation, while L1c3 does not.

In some scenarios, the same location bounces back and forth. Should it still be counted as a haplogroup defining mutation, or is it simply “noise”?

Heteroplasmies

How do heteroplasmies play into this scenario?

Heteroplasmies occur when more than one value is discerned in an individual’s DNA at a specific location. Heteroplasmies do not define haplogroups, but they are reported in your personal results.

To be reported as a heteroplasmy, both values need to be detected at a level of over 20%. In the above scenario, if both G and A were found greater than 20% of the time, it would be counted at a heteroplasmy with a special notation.

For example, if G and A are both found more than 20% of the time, the notation would be R instead of either G or A. If the location was G7055, above, and G and A were both found above 20%, the notation would be G7055R.

However, if G was found 81% of the time or more, then it would be counted as G, which is “normal,” and if A was found 81% of the time or more, then the value would be reported as A, a mutation. If we see the normal state of G, then an A, then a G, is that a mutation and a back mutation? How many samples would need to contain that back mutation to count it as a mutation and not an aberration, an undetected borderline heteroplasmy slipping back and forth over the threshold, or simply noise?

Transitions Versus Transversions

There are two types of mutations, transitions and transversions, that probably should be weighted differently – but how differently, and why?

Some types of mutations occur more easily than others and are therefore more common. Paul explains this very well in his RootsTech video, but in a nutshell, transitions between T/C and A/G are much more common than transversions between A/C, G/T, C/G, and A/T. Therefore, transversions are noted with a small letter, shown above as T7624a.

In phylogenetics, the rarer mutation which is chemically less likely to occur (transversion) is weighted more heavily than the likelier mutations (transitions).

Insertions

Insertions are another type of challenge. Insertions happen when extra DNA is inserted at a specific location, kind of like the genetic equivalent of cutting in line.

In this graphic, we see that at location 5899, there’s an extension of .XC, written as 5899.XC. This means that at this location, you’ll find an unknown or varying number of additional Cs inserted. Paul showed several example sequences in the box at upper left. In some people who have this mutation, there are only one or two inserted Cs. In other people, there are several Cs, shown in the bottom two sequences.

You might recognize this as a phenomenon similar to Y DNA STRs which are short tandem repeats. Of course, we don’t use STRs for haplogroup identification in Y DNA. How should we handle insertions, especially multiple insertions, in building the Mitotree?

Deletions

We see deletions of DNA too, indicated by a small “d” after the location. In some cases, we find large deletions.

At location 8281, there is a 9 base-pair deletion (8281 through 8289) that is one of the haplogroup defining mutations for haplogroup L0a2. We find a 9 base-pair deletion in exactly the same location again within subclades of haplogroups B and U.

Is there something about this specific location that makes it more prone to deletions, and specifically a deletion of exactly 9 base pairs?

Seeking Answers

Of course, we’re seeking all of these answers.

The team has been writing code to create structural trees based on various scenarios and trying to determine which ones make the most sense, all factors considered.

The current official tree, meaning the 2016 Build 17 version of Phylotree, is based on about 8,000 samples. Working with one million versus 8,000 is a challenge that ramps exponentially, necessitating substantial computing power.

Working with 125 times more data provides amazing potential, but it has also introduced challenges that never had to be addressed before. It’s evident, to us at least, why Phylotree wasn’t updated after 2016. The tools simply don’t exist.

Sneak Peek

We fully expect hundreds if not thousands of new haplogroups to form. Today, Paul’s haplogroup is U5a2b2a which was formed about 5,000 years ago during the Bronze Age.

The haplogroup itself is useful to determine roughly where your ancestors were at that time, and often provide information about more recent population group history, but you need mitochondrial DNA matching to provide more genealogically useful information.

Paul’s test results show that he has 8 extra mutations, which means those mutations are in addition to his haplogroup-defining mutations. These extra mutations are what make genealogical matching so useful.

Paul has 16 full sequence matches that match him at a genetic distance of 3 mutations or less, although due to privacy restrictions at FamilyTreeDNA, we can’t see which matches share which mutations.

Given that Paul has 8 extra mutations, this means that it’s possible that one or more new haplogroups will be formed using some or all of those 8 extra mutations, and that those people who match him at a GD of 3 or less will very likely be members of a newly formed haplogroup.

Here’s a comparison of Paul’s haplogroup today, at left, with the newly created U5a2b2a branch and resulting subclades in a beta version of our experimental Mitotree, at right. This moves Paul’s new haplogroup, the pink node at right, from 5,000 to 500 years ago which is clearly within a genealogically relevant timeframe.

The single haplogroup, U5a2b2a, now has been expanded to 7 subgroups. If U5a2b2a is representative of the expansion capability of the entire tree, that’s a 7-fold increase.

Of Paul’s 16 matches, those with the same new haplogroup are those where he needs to focus his genealogical research.

Where Are We?

This is not a commitment, but we expect to release a sneak preview of the new Mitotree this year.

If you have extra or missing mutations, especially in the coding region, you and your close matches may very well receive a new, expanded haplogroup.

Highly refined haplogroups will improve the ability to use mitochondrial DNA for genealogical purposes – similar to what the Big Y-700 SNP testing and the expanded haplotree have done for Y DNA.

Like with Y DNA, you’ll want to use your new haplogroup in combination with genealogical trees.

The more people that test, the more success stories emerge, and the more people that WILL test. Just think what would happen if everyone who took a Y or autosomal DNA test also took a mitochondrial DNA test. We’d be bulldozing through brick walls every day.

I don’t know about you, but I have so many women in my trees with no parents. I need more tools and can hardly wait.

Resources

The new Mitotree is fueled by the Million Mito Project which is fueled by full sequence DNA testing, so please purchase yours today.

And yes, in case you were wondering, the new Mitotree will be free and public, just like the existing Mitochondrial DNA Tree and Y DNA Tree are at FamilyTreeDNA today.

You can read more about the Million Mito project here and here.

You can watch Paul’s Million Mito RootsTech presentation, here.

Paul, Miguel and I will be co-presenting Mitochondrial DNA Academy on Saturday, April 23, during the ECCGC Conference which you can read about here and register here.

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Top Ten RootsTech 2022 DNA Sessions + All DNA Session Links

The official dates of RootsTech 2022 were March 3-5, but the sessions and content in the vendor booths are still available. I’ve compiled a list of the sessions focused on DNA, with web links on the RootsTech YouTube channel

YouTube reports the number of views, so I was able to compile that information as of March 8, 2022.

I do want to explain a couple of things to add context to the numbers.

Most speakers recorded their sessions, but a few offered live sessions which were recorded, then posted later for participants to view. However, there have been glitches in that process. While the sessions were anticipated to be available an hour or so later, that didn’t quite happen, and a couple still aren’t posted. I’m sure the presenters are distressed by this, so be sure to watch those when they are up and running.

The Zoom rooms where participants gathered for the live sessions were restricted to 500 attendees. The YouTube number of views does not include the number of live viewers, so you’ll need to add an additional number, up to 500.

When you see a number before the session name, whether recorded or live, that means that the session is part of a series. RootsTech required speakers to divide longer sessions into a series of shorter sessions no longer than 15-20 minutes each. The goal was for viewers to be able to watch the sessions one after the other, as one class, or separately, and still make sense of the content. Let’s just say this was the most challenging thing I’ve ever done as a presenter.

For recorded series sessions, these are posted as 1, 2 and 3, as you can see below with Diahan Southard’s sessions. However, with my live session series, that didn’t happen. It looks like my sessions are a series, but when you watch them, parts 1, 2 and 3 are recorded and presented as one session. Personally, I’m fine with this, because I think the information makes a lot more sense this way. However, it makes comparisons difficult.

This was only the second year for RootsTech to be virtual and the conference is absolutely HUGE, so live and learn. Next year will be smoother and hopefully, at least partially in-person too.

When I “arrived” to present my live session, “Associating Autosomal DNA Segments With Ancestors,” my lovely moderator, Rhett, told me that they were going to livestream my session to the RootsTech page on Facebook as well because they realized that the 500 Zoom seat limit had been a problem the day before with some popular sessions. I have about 9000 views for that session and more than 7,400 of them are on the RootsTech Facebook page – and that was WITHOUT any advance notice or advertising. I know that the Zoom room was full in addition. I felt kind of strange about including my results in the top ten because I had that advantage, but I didn’t know quite how to otherwise count my session. As it turns out, all sessions with more than 1000 views made it into the top ten so mine would have been there one way or another. A big thank you to everyone who watched!

I hope that the RootsTech team notices that the most viewed session is the one that was NOT constrained by the 500-seat limited AND was live-streamed on Facebook. Seems like this might be a great way to increase session views for everyone next year. Hint, hint!!!

I also want to say a huge thank you to all of the presenters for producing outstanding content. The sessions were challenging to find, plus RootsTech is always hectic, even virtually. So, I know a LOT of people will want to view these informative sessions, now that you know where to look and have more time. Please remember to “like” the session on YouTube as a way of thanking your presenter.

With 140 DNA-focused sessions available, you can watch a new session, and put it to use, every other day for the next year! How fun is that! You can use this article as your own playlist.

Please feel free to share this article with your friends and genealogy groups so everyone can learn more about using DNA for genealogy.

Ok, let’s look at the top 10. Drum roll please…

Top 10 Most Viewed RootsTech Sessions

Session Title Presenter YouTube Link Views
1 1. Associating Autosomal DNA Segments With Ancestors Roberta Estes (live) https://www.youtube.com/watch?v=_IHSCkNnX48

 

~9000: 1019 + 500 live viewers + 7,400+ Facebook
2 1. What to Do with Your DNA Test Results in 2022 (part 1 of 3) Diahan Southard https://www.youtube.com/watch?v=FENAKAYLXX4 7428
3 Who Is FamilyTreeDNA? FamilyTreeDNA – Bennett Greenspan https://www.youtube.com/watch?v=MHFtwoatJ-A 2946
4 2. What to Do with Your DNA Test Results in 2022 (part 2 of 3) Diahan Southard https://www.youtube.com/watch?v=mIllhtONhlI 2448
5 Latest DNA Painter Releases DNAPainter Jonny Perl (live) https://www.youtube.com/watch?v=iLBThU8l33o 2230 + live viewers
6 DNA Painter Introduction DNAPainter – Jonny Perl https://www.youtube.com/watch?v=Rpe5LMPNmf0 1983
7 3. What to Do with Your DNA Test Results in 2022 (part 3 of 3) Diahan Southard https://www.youtube.com/watch?v=hemY5TuLmGI 1780
8 The Tree of Mankind Age Estimates Paul Maier https://www.youtube.com/watch?v=jjkL8PWAEwk 1638
9 A Sneak Peek at FamilyTreeDNA Coming Attractions FamilyTreeDNA (live) https://www.youtube.com/watch?v=K9sKqNScvnE 1270 + live viewers

 

10 Extending Time Horizons with DNA Rob Spencer (live) https://www.youtube.com/watch?v=wppXD1Zz2sQ 1037 + live viewers

 

All DNA-Focused Sessions

I know you’ll find LOTS of goodies here. Which ones are your favorites?

  Session Presenter YouTube Link Views
1 Estimating Relationships by Combining DNA from Multiple Siblings Amy Williams https://www.youtube.com/watch?v=xs1U0ohpKSA 201
2 Overview of HAPI-DNA.org Amy Williams https://www.youtube.com/watch?v=FjNiJgWaBeQ 126
3 How do AncestryDNA® Communities help tell your story? | Ancestry® Ancestry https://www.youtube.com/watch?v=EQNpUxonQO4 183

 

4 AncestryDNA® 201 Ancestry – Crista Cowan https://www.youtube.com/watch?v=lbqpnXloM5s

 

494
5 Genealogy in a Minute: Increase Discoveries by Attaching AncestryDNA® Results to Family Tree Ancestry – Crista Cowan https://www.youtube.com/watch?v=iAqwSCO8Pvw 369
6 AncestryDNA® 101: Beginner’s Guide to AncestryDNA® | Ancestry® Ancestry – Lisa Elzey https://www.youtube.com/watch?v=-N2usCR86sY 909
7 Hidden in Plain Sight: Free People of Color in Your Family Tree Cheri Daniels https://www.youtube.com/watch?v=FUOcdhO3uDM 179
8 Finding Relatives to Prevent Hereditary Cancer ConnectMyVariant – Dr. Brian Shirts https://www.youtube.com/watch?v=LpwLGgEp2IE 63
9 Piling on the chromosomes Debbie Kennett https://www.youtube.com/watch?v=e14lMsS3rcY 465
10 Linking Families With Rare Genetic Condition Using Genealogy Deborah Neklason https://www.youtube.com/watch?v=b94lUfeAw9k 43
11 1. What to Do with Your DNA Test Results in 2022 Diahan Southard https://www.youtube.com/watch?v=FENAKAYLXX4 7428
12 1. What to Do with Your DNA Test Results in 2022 Diahan Southard https://www.youtube.com/watch?v=hemY5TuLmGI 1780
13 2. What to Do with Your DNA Test Results in 2022 Diahan Southard https://www.youtube.com/watch?v=mIllhtONhlI 2448
14 DNA Testing For Family History Diahan Southard https://www.youtube.com/watch?v=kCLuOCC924s 84

 

15 Understanding Your DNA Ethnicity Estimate at 23andMe Diana Elder

 

https://www.youtube.com/watch?v=xT1OtyvbVHE 66
16 Understanding Your Ethnicity Estimate at FamilyTreeDNA Diana Elder https://www.youtube.com/watch?v=XosjViloVE0 73
17 DNA Monkey Wrenches Katherine Borges https://www.youtube.com/watch?v=Thv79pmII5M 245
18 Advanced Features in your Ancestral Tree and Fan Chart DNAPainter – Jonny Perl https://www.youtube.com/watch?v=4u5Vf13ZoAc 425
19 DNA Painter Introduction DNAPainter – Jonny Perl https://www.youtube.com/watch?v=Rpe5LMPNmf0 1983
20 Getting Segment Data from 23andMe DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=8EBRI85P3KQ 134
21 Getting segment data from FamilyTreeDNA DNA matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=rWnxK86a12U 169
22 Getting segment data from Gedmatch DNA matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=WF11HEL8Apk 163
23 Getting segment data from Geneanet DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=eclj8Ap0uK4 38
24 Getting segment data from MyHeritage DNA matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=9rGwOtqbg5E 160
25 Inferred Chromosome Mapping: Maximize your DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=tzd5arHkv64 688
26 Keeping track of your genetic family tree in a fan chart DNAPainter – Jonny Perl https://www.youtube.com/watch?v=W3Hcno7en94 806

 

27 Mapping a DNA Match in a Chromosome Map DNAPainter – Jonny Perl https://www.youtube.com/watch?v=A61zQFBWaiY 423
28 Setting up an Ancestral Tree and Fan Chart and Exploring Tree Completeness DNAPainter – Jonny Perl https://www.youtube.com/watch?v=lkJp5Xk1thg 77
29 Using the Shared cM Project Tool to Evaluate DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=vxhn9l3Dxg4 763
30 Your First Chromosome Map: Using your DNA Matches to Link Segments to Ancestors DNAPainter – Jonny Perl https://www.youtube.com/watch?v=tzd5arHkv64 688
31 DNA Painter for absolute beginners DNAPainter (Jonny Perl) https://www.youtube.com/watch?v=JwUWW4WHwhk 1196
32 Latest DNA Painter Releases DNAPainter (live) https://www.youtube.com/watch?v=iLBThU8l33o 2230 + live viewers
33 Unraveling your genealogy with DNA segment networks using AutoSegment from Genetic Affairs Evert-Jan Blom https://www.youtube.com/watch?v=rVpsJSqOJZI

 

162
34 Unraveling your genealogy with genetic networks using AutoCluster Evert-Jan Blom https://www.youtube.com/watch?v=ZTKSz_X7_zs 201

 

 

35 Unraveling your genealogy with reconstructed trees using AutoTree & AutoKinship from Genetic Affairs Evert-Jan Blom https://www.youtube.com/watch?v=OmDQoAn9tVw 143
36 Research Like a Pro with DNA – A Genealogist’s Guide to Finding and Confirming Ancestors with DNA Family Locket Genealogists https://www.youtube.com/watch?v=NYpLscJJQyk 183
37 How to Interpret a DNA Network Graph Family Locket Genealogists – Diana Elder https://www.youtube.com/watch?v=i83WRl1uLWY 393
38 Find and Confirm Ancestors with DNA Evidence Family Locket Genealogists – Nicole Dyer https://www.youtube.com/watch?v=DGLpV3aNuZI 144
39 How To Make A DNA Network Graph Family Locket Genealogists – Nicole Dyer https://www.youtube.com/watch?v=MLm_dVK2kAA 201
40 Create A Family Tree With Your DNA Matches-Use Lucidchart To Create A Picture Worth A Thousand Words Family Locket Genealogists – Robin Wirthlin https://www.youtube.com/watch?v=RlRIzcW-JI4 270
41 Charting Companion 7 – DNA Edition Family Tree Maker https://www.youtube.com/watch?v=k2r9rkk22nU 316

 

42 Family Finder Chromosome Browser: How to Use FamilyTreeDNA https://www.youtube.com/watch?v=w0_tgopBn_o 750

 

 

43 FamilyTreeDNA: 22 Years of Breaking Down Brick Walls FamilyTreeDNA https://www.familysearch.org/rootstech/session/familytreedna-22-years-of-breaking-down-brick-walls Not available
44 Review of Autosomal DNA, Y-DNA, & mtDNA FamilyTreeDNA  – Janine Cloud https://www.youtube.com/watch?v=EJoQVKxgaVY 77
45 Who Is FamilyTreeDNA? FamilyTreeDNA – Bennett Greenspan https://www.youtube.com/watch?v=MHFtwoatJ-A 2946
46 Part 1: How to Interpret Y-DNA Results, A Walk Through the Big Y FamilyTreeDNA – Casimir Roman https://www.youtube.com/watch?v=ra1cjGgvhRw 684

 

47 Part 2: How to Interpret Y-DNA Results, A Walk Through the Big Y FamilyTreeDNA – Casimir Roman https://www.youtube.com/watch?v=CgqcjBD6N8Y

 

259
48 Big Y-700: A Brief Overview FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=IefUipZcLCQ 96
49 Mitochondrial DNA & The Million Mito Project FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=5Zppv2uAa6I 179
50 Mitochondrial DNA: What is a Heteroplasmy FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=ZeGTyUDKySk 57
51 Y-DNA Big Y: A Lifetime Analysis FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=E6NEU92rpiM 154
52 Y-DNA: How SNPs Are Added to the Y Haplotree FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=CGQaYcroRwY 220
53 Family Finder myOrigins: Beginner’s Guide FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=VrJNpSv8nlA 88
54 Mitochondrial DNA: Matches Map & Results for mtDNA FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=YtA1j01MOvs 190
55 Mitochondrial DNA: mtDNA Mutations Explained FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=awPs0cmZApE 340

 

56 Y-DNA: Haplotree and SNPs Page Overview FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=FOuVhoMD-hw 432
57 Y-DNA: Understanding the Y-STR Results Page FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=gCeZz1rQplI 148
58 Y-DNA: What Is Genetic Distance? FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=qJ6wY6ILhfg 149
59 DNA Tools: myOrigins 3.0 Explained, Part 1 FamilyTreeDNA – Paul Maier https://www.youtube.com/watch?v=ACgY3F4-w78 74

 

60 DNA Tools: myOrigins 3.0 Explained, Part 2 FamilyTreeDNA – Paul Maier https://www.youtube.com/watch?v=h7qU36bIFg0 50
61 DNA Tools: myOrigins 3.0 Explained, Part 3 FamilyTreeDNA – Paul Maier https://www.youtube.com/watch?v=SWlGPm8BGyU 36
62 African American Genealogy Research Tips FamilyTreeDNA – Sherman McRae https://www.youtube.com/watch?v=XdbkM58rXIQ 153

 

63 Connecting With My Ancestors Through Y-DNA FamilyTreeDNA – Sherman McRae https://www.youtube.com/watch?v=xbo1XnLkuQU 200
64 Join The Million Mito Project FamilyTreeDNA (Join link) https://www.familysearch.org/rootstech/session/join-the-million-mito-project link
65 View the World’s Largest mtDNA Haplotree FamilyTreeDNA (Link to mtDNA tree) https://www.familytreedna.com/public/mt-dna-haplotree/L n/a
66 View the World’s Largest Y Haplotree FamilyTreeDNA (Link to Y tree) https://www.familytreedna.com/public/y-dna-haplotree/A link
67 A Sneak Peek at FamilyTreeDNA Coming Attractions FamilyTreeDNA (live) https://www.youtube.com/watch?v=K9sKqNScvnE 1270 + live viewers

 

68 DNA Upload: How to Transfer Your Autosomal DNA Data FamilyTreeDNA -Katy Rowe https://www.youtube.com/watch?v=CS-rH_HrGlo 303
69 Family Finder myOrigins: How to Compare Origins With Your DNA Matches FamilyTreeDNA -Katy Rowe https://www.youtube.com/watch?v=7mBmWhM4j9Y 145
70 Join Group Projects at FamilyTreeDNA FamilyTreeDNA link to learning center article) https://www.familysearch.org/rootstech/session/join-group-projects-at-familytreedna link

 

71 Product Demo – Unraveling your genealogy with reconstructed trees using AutoKinship GEDmatch https://www.youtube.com/watch?v=R7_W0FM5U7c 803
72 Towards a Genetic Genealogy Driven Irish Reference Genome Gerard Corcoran https://www.youtube.com/watch?v=6Kx8qeNiVmo 155

 

73 Discovering Biological Origins in Chile With DNA: Simple Triangulation Gonzalo Alexis Luengo Orellana https://www.youtube.com/watch?v=WcVby54Uigc 40
74 Cousin Lynne: An Adoption Story International Association of Jewish Genealogical Societies https://www.youtube.com/watch?v=AptMcV4_B4o 111
75 Using DNA Testing to Uncover Native Ancestry Janine Cloud https://www.youtube.com/watch?v=edzebJXepMA 205
76 1. Forensic Genetic Genealogy Jarrett Ross https://www.youtube.com/watch?v=0euIDZTmx5g 58
77 Reunited and it Feels so Good Jennifer Mendelsohn https://www.youtube.com/watch?v=X-hxjm7grBE 57

 

78 Genealogical Research and DNA Testing: The Perfect Companions Kimberly Brown https://www.youtube.com/watch?v=X82jA3xUVXk 80
79 Finding a Jewish Sperm Donor Kitty Munson Cooper https://www.youtube.com/watch?v=iKRjFfNcpug 164
80 Using DNA in South African Genealogy Linda Farrell https://www.youtube.com/watch?v=HXkbBWmORM0 141
81 Using DNA Group Projects In Your Family History Research Mags Gaulden https://www.youtube.com/watch?v=0tX7QDib4Cw 165
82 2. The Expansion of Genealogy Into Forensics Marybeth Sciaretta https://www.youtube.com/watch?v=HcEO-rMe3Xo 35

 

83 DNA Interest Groups That Keep ’em Coming Back McKell Keeney (live) https://www.youtube.com/watch?v=HFwpmtA_QbE 180 plus live viewers
84 Searching for Close Relatives with Your DNA Results Mckell Keeney (live) https://www.familysearch.org/rootstech/session/searching-for-close-relatives-with-your-dna-results Not yet available
85 Top Ten Reasons To DNA Test For Family History Michelle Leonard https://www.youtube.com/watch?v=1B9hEeu_dic 181
86 Top Tips For Identifying DNA Matches Michelle Leonard https://www.youtube.com/watch?v=-3Oay_btNAI 306
87 Maximising Messages Michelle Patient https://www.youtube.com/watch?v=4TRmn0qzHik 442
88 How to Filter and Sort Your DNA Matches MyHeritage https://www.youtube.com/watch?v=fmIgamFDvc8 88
89 How to Get Started with Your DNA Matches MyHeritage https://www.youtube.com/watch?v=JPOzhTxhU0E 447

 

90 How to Track DNA Kits in MyHeritage` MyHeritage https://www.youtube.com/watch?v=2W0zBbkBJ5w 28

 

91 How to Upload Your DNA Data to MyHeritage MyHeritage https://www.youtube.com/watch?v=nJ4RoZOQafY 82
92 How to Use Genetic Groups MyHeritage https://www.youtube.com/watch?v=PtDAUHN-3-4 62
My Story: Hope MyHeritage https://www.youtube.com/watch?v=qjyggKZEXYA 133
93 MyHeritage Keynote, RootsTech 2022 MyHeritage https://www.familysearch.org/rootstech/session/myheritage-keynote-rootstech-2022 Not available
94 Using Labels to Name Your DNA Match List MyHeritage https://www.youtube.com/watch?v=enJjdw1xlsk 139

 

95 An Introduction to DNA on MyHeritage MyHeritage – Daniel Horowitz https://www.youtube.com/watch?v=1I6LHezMkgc 60
96 Using MyHeritage’s Advanced DNA Tools to Shed Light on Your DNA Matches MyHeritage – Daniel Horowitz https://www.youtube.com/watch?v=Pez46Xw20b4 110
97 You’ve Got DNA Matches! Now What? MyHeritage – Daniel Horowitz https://www.youtube.com/watch?v=gl3UVksA-2E 260
98 My Story: Lizzie and Ayla MyHeritage – Elizbeth Shaltz https://www.youtube.com/watch?v=NQv6C8G39Kw 147
99 My Story: Fernando and Iwen MyHeritage – Fernando Hermansson https://www.youtube.com/watch?v=98-AR0M7fFE 165

 

100 Using the Autocluster and the Chromosome Browser to Explore Your DNA Matches MyHeritage – Gal Zruhen https://www.youtube.com/watch?v=a7aQbfP7lWU 115

 

101 My Story : Kara Ashby Utah Wedding MyHeritage – Kara Ashby https://www.youtube.com/watch?v=Qbr_gg1sDRo 200
102 When Harry Met Dotty – using DNA to break down brick walls Nick David Barratt https://www.youtube.com/watch?v=8SdnLuwWpJs 679
103 How to Add a DNA Match to Airtable Nicole Dyer https://www.youtube.com/watch?v=oKxizWIOKC0 161
104 How to Download DNA Match Lists with DNAGedcom Client Nicole Dyer https://www.youtube.com/watch?v=t9zTWnwl98E 124
105 How to Know if a Matching DNA Segment is Maternal or Paternal Nicole Dyer https://www.youtube.com/watch?v=-zd5iat7pmg 161
106 DNA Basics Part I Centimorgans and Family Relationships Origins International, Inc. dba Origins Genealogy https://www.youtube.com/watch?v=SI1yUdnSpHA 372
107 DNA Basics Part II Clustering and Connecting Your DNA Matches Origins International, Inc. dba Origins Genealogy https://www.youtube.com/watch?v=ECs4a1hwGcs 333
108 DNA Basics Part III Charting Your DNA Matches to Get Answers Origins International, Inc. dba Origins Genealogy https://www.youtube.com/watch?v=qzybjN0JBGY 270
109 2. Using Cluster Auto Painter Patricia Coleman https://www.youtube.com/watch?v=-nfLixwxKN4 691
110 3. Using Online Irish Records Patricia Coleman https://www.youtube.com/watch?v=mZsB0l4z4os 802
111 Exploring Different Types of Clusters Patricia Coleman https://www.youtube.com/watch?v=eEZBFPC8aL4 972

 

112 The Million Mito Project: Growing the Family Tree of Womankind Paul Maier https://www.youtube.com/watch?v=cpctoeKb0Kw 541
113 The Tree of Mankind Age Estimates Paul Maier https://www.youtube.com/watch?v=jjkL8PWAEwk 1638
114 Y-DNA and Mitochondrial DNA Testing Plans Paul Woodbury https://www.youtube.com/watch?v=akymSm0QKaY 168
115 Finding Biological Family Price Genealogy https://www.youtube.com/watch?v=4xh-r3hZ6Hw 137
116 What Y-DNA Testing Can Do for You Richard Hill https://www.youtube.com/watch?v=a094YhIY4HU 191
117 Extending Time Horizons with DNA Rob Spencer (live) https://www.youtube.com/watch?v=wppXD1Zz2sQ 1037 + live viewers
118 DNA for Native American Ancestry by Roberta Estes Roberta Estes https://www.youtube.com/watch?v=EbNyXCFfp4M 212
119 1. Associating Autosomal DNA Segments With Ancestors Roberta Estes (live) https://www.youtube.com/watch?v=_IHSCkNnX48

 

~9000: 1019 + 500 live viewers + 7,400+ Facebook
120 1. What Can I Do With Ancestral DNA Segments? Roberta Estes (live) https://www.youtube.com/watch?v=Suv3l4iZYAQ 325 plus live viewers

 

121 Native American DNA – Ancient and Contemporary Maps Roberta Estes (live) https://www.youtube.com/watch?v=dFTl2vXUz_0 212 plus 483 live viewers

 

122 How Can DNA Enhance My Family History Research? Robin Wirthlin https://www.youtube.com/watch?v=f3KKW-U2P6w 102
123 How to Analyze a DNA Match Robin Wirthlin https://www.youtube.com/watch?v=LTL8NbpROwM 367
124 1. Jewish Ethnicity & DNA: History, Migration, Genetics Schelly Talalay Dardashti https://www.youtube.com/watch?v=AIJyphGEZTA 82

 

125 2. Jewish Ethnicity & DNA: History, Migration, Genetics Schelly Talalay Dardashti https://www.youtube.com/watch?v=VM3MCYM0hkI 72
126 Ask us about DNA Talking Family History (live) https://www.youtube.com/watch?v=kv_RfR6OPpU 96 plus live viewers
127 1. An Introduction to Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=WNhErW5UVKU

 

183
128 2. An Introduction to Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=CRpQ8EVOShI 110

 

129 Common Problems When Doing Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=hzFxtBS5a8Y 68
130 Cross Visual Phasing to Go Back Another Generation Tanner Blair Tolman https://www.youtube.com/watch?v=MrrMqhfiwbs 64
131 DNA Basics Tanner Blair Tolman https://www.youtube.com/watch?v=OCMUz-kXNZc 155
132 DNA Painter and Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=2-eh1L4wOmQ 155
133 DNA Painter Part 2: Chromosome Mapping Tanner Blair Tolman https://www.youtube.com/watch?v=zgOJDRG7hJc 172
134 DNA Painter Part 3: The Inferred Segment Generator Tanner Blair Tolman https://www.youtube.com/watch?v=96ai8nM4lzo

 

100
135 DNA Painter Part 4: The Distinct Segment Generator Tanner Blair Tolman https://www.youtube.com/watch?v=Pu-WIEQ_8vc 83
136 DNA Painter Part 5: Ancestral Trees Tanner Blair Tolman https://www.youtube.com/watch?v=dkYDeFLduKA 73
137 Understanding Your DNA Ethnicity Results Tanner Blair Tolman https://www.youtube.com/watch?v=4tAd8jK6Bgw 518
138 What’s New at GEDmatch Tim Janzen https://www.youtube.com/watch?v=AjA59BG_cF4

 

515
139 What Does it Mean to Have Neanderthal Ancestry? Ugo Perego https://www.youtube.com/watch?v=DshCKDW07so 190
140 Big Y-700 Your DNA Guide https://www.youtube.com/watch?v=rIFC69qswiA 143
141 Next Steps with Your DNA Your DNA Guide – Diahan Southard (live) https://www.familysearch.org/rootstech/session/next-steps-with-your-dna Not yet available

Additions:

142  Adventures of an Amateur Genetic Genealogist – Geoff Nelson https://www.familysearch.org/rootstech/session/adventures-of-an-amateur-genetic-genealogist     291 views

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FamilyTreeDNA Keynote, RootsTech Wrap + Special Show Pricing Still Available

Am I ever whipped. My two live Sessions that were actually a series of three classes each took place on Friday. Yes, that means I presented 6 sessions on Friday, complete with a couple of Zoom gremlins, of course. It’s the nature of the time we live in.

RootsTech tried something new that they’ve never done before. The Zoom class sessions were restricted to 500 attendees each. RootsTech was concerned about disappointed attendees when the room was full and they couldn’t get in, so we live-streamed three of my sessions to Facebook in addition to the 500 Zoom seats.

As of this evening, 6,800 of you have viewed the Facebook video, “Associating Autosomal DNA Segments With Ancestors.” I’m stunned, and touched. Thank you, thank you. Here’s the Facebook link, and here’s the RootsTech YouTube link.

My afternoon sessions, “What Can I DO With Ancestral DNA Segments?” can be viewed here at RootsTech or here on YouTube.

I must admit, I’m really, REALLY looking forward to being together again because RootsTech without the socializing and in-person Expo Hall just isn’t the same. Still, be sure to take a virtual walk through the Expo Hall, here. There’s lots of content in the vendors” booths and it will remain available for all of 2022, until the beginning of RootsTech 2023..

Between prep for my classes and presenting, I didn’t have a lot of time to watch other sessions, but I was able to catch the FamilyTreeDNA keynote and their 2022 Product Sneak Peek. Both were quite worthwhile.

However, I just realized that FamilyTreeDNA’s special show pricing promo codes are still valid for the next two days.

 Special Prices Are Still Available

Every single test that FamilyTreeDNA offers, including UPGRADES, is on sale right now by using special RootsTech promo codes. These prices are good for two more days, through March 7th, so if you want to purchase a Y DNA test, mitochondrial, or Family Finder autosomal test, or upgrade, click here to see the prices only available at RootsTech (and to you through my blog.) It’s not too late, but it will be soon.

To order, click here to sign on or place your order.

FamilyTreeDNA’s Keynote

FamilyTreeDNA’s keynote was titled FamilyTreeDNA: 22 Years of Breaking Down Brick Walls.

I really enjoyed this session, in part because I’ve been a part of the genetic genealogy revolution and evolution from the beginning. Not only that, but I know every single person they interviewed for this video, and have for years. If you’ve been participating in genetic genealogy for some time, you’ll know many of these people too. For a minute, it was almost as good as visiting in person.

I’m going to share a few highlights from the session, but I’m also going to include information NOT in the video. I was one of the early project administrators, so I’ve been along for the ride for just a few months shy of 22 years.

FamilyTreeDNA was the first US company to enter the DNA testing space, the first to offer Y DNA testing, and the only one of the early companies that remains viable today. FamilyTreeDNA was the result of Bennett Greenspan’s dream – but initially, he was only dreaming small. Just like any other genealogist – he was dreaming about breaking down a brick wall which he explains in the video.

I’m so VERY grateful that Bennett had that dream, and persisted, because it means that now millions of us can do the same – and will into the future.

Bennett tells this better than anyone else, along with his partner, Max Blankfeld.

“Some people were fascinated,” Bennett said.

Yep, that’s for sure! I certainly was.

“Among the first genetic genealogists in the world.”

“Frontier of the genetic genealogy revolution.”

Indeed, we were and still are. Today’s genetic genealogy industry wouldn’t even exist were it not for FamilyTreeDNA and their early testers.

I love Max Blankfeld’s story of their first office, and you will too.

This IS the quintessential story of entrepreneurship.

In 2004, when FamilyTreeDNA was only four years old, they hosted the very first annual international project administrator’s conference. At that time, it was believed that the only people that would be interested in learning at that level and would attend a DNA conference would be project administrators who were managing surname and regional projects. How times have changed! This week at RootsTech, we probably had more people viewing DNA sessions than people that had tested altogether in 2004. I purchased kit number 30,087 on December 28, 2004, and kit 50,000 a year later on New Year’s Eve right at midnight!

In April 2005, Nat Geo partnered with FamilyTreeDNA and founded the Genographic Project which was scheduled to last for 5 years. They were hoping to attract 100,000 people who would be willing to test their DNA to discover their roots – and along with that – our human roots. The Genographic Project would run for an incredible 15 years.

In 2005 when the second Project Administrator’s conference was held at the National Geographic Society headquarters in Washington DC, I don’t think any of us realized the historic nature of the moment we were participating in.

I remember walking from my hotel, ironically named “Helix,” to that iconic building. I had spent my childhood reading those yellow magazines at school and dreaming of far-away places. As an adult, I had been a life-long subscriber. Never, in my wildest dreams did I imagine ever visiting Nat Geo and walking the marble Explorer’s Hall with the portraits of the founders and early explorers hanging above and keeping a watchful eye on us. We would not disappoint them.

That 100,000 participation goal was quickly reached, within weeks, and surpassed, leading us all to walk the road towards the building that housed the Explorer’s Hall, Explorers’ in Residence, and so much more.

We were all explorers, pioneers, adventurers seeking to use the DNA from our ancestors in the past to identify who they were. Using futuristic technology tools like a mirror to look backward into the dim recesses of the past.

The archaeology being unearthed and studied was no longer at the ends of the earth but within our own bodies. The final frontier. Reaching out to explore meant reaching inward, and backward in time, using the most progressive technology of the day.

Most of the administrators in attendance, all volunteers, were on a first-name basis with each other and also with Max, Bennett, and the scientists.

Here, Bennett with a member of the science team from the University of Arizona describes future research goals. Every year FamilyTreeDNA has improved its products in numerous ways.

Today, that small startup business has its own ground-breaking state-of-the-art lab. More than 10,000 DNA projects are still administered by passionate volunteer administrators who focus on what they seek – such as the history of their surname or a specific haplogroup. Their world-class lab allows FamilyTreeDNA to focus on research and science in addition to DNA processing. The lab allows constant improvement so their three types of genetic genealogy products, Y, mitochondrial and autosomal DNA.

Those three types of tests combine to provide genealogical insights and solutions. The more the science improves, the more solutions can and will be found.

If you watch the video, you’ll see 6 people who have solved particularly difficult and thorny problems. We are all long-time project administrators, all participate on a daily basis in this field and community – and all have an undying love for both genealogy and genetic genealogy.

You’ll recognize most of these people, including yours truly.

  • I talk about my mother’s heritage, unveiled through mitochondrial DNA.
  • Rob Warthen speaks about receiving a random phone call from another genealogist as his introduction to genetic genealogy. Later, he purchased a DNA test for his girlfriend, an adoptee, for Christmas and sweetened the deal by offering to “go where you’re from” for vacation. He didn’t realize why she was moved to tears – that test revealed the first piece of information she had ever known about her history. DNA changed her and Rob’s life. He eventually identified her birth parents – and went on to found both DNAAdoption.org and DNAGedcom.
  • Richard Hill was adopted and began his search in his 30s, but it would be DNA that ended his search. His moving story is told in his book, Finding Family: My Search for Roots and the Secrets in My DNA.
  • Mags Gaulden, professional genealogist and founder of Grandma’s Genes and MitoYDNA.org tells about her 91-year-old adopted client who had given up all hope of discovering her roots. Back in the 1950s, there was literally nothing in her client’s adoption file. She was reconciled to the fact that “I would never know who I was.” Mags simply could not accept that and 2 years later, Mags found her parents’ names.

  • Lara Diamond’s family was decimated during the holocaust. Lara’s family thought everyone in her grandfather’s family had been killed, but in 2013, autosomal DNA testing let her to her grandfather’s aunt who was not killed in the holocaust as everyone thought. The aunt and first cousin were living in Detroit. Lara went from almost no family to a family reunion, shown above. She says she finally met “people who look like me.”
  • Katherine Borges founded ISOGG.org, the International Society of Genetic Genealogy in 2005, following the first genetic genealogy conference in late 2004 where she realized that the genealogy community desperately needed education – beginning with DNA terms. I remember her jokingly standing in the hallway saying that she understood three words, “a, and and the.” While that’s cute today, it was real at that time because DNA was a foreign language, technology, and concept to genealogy. In fact, for years we were banned from discussing the topic on RootsWeb. The consummate genetic genealogist, Katherine carries DNA kits in her purse, even to Scotland!

Bennett says that he’s excited about the future, for the next generation of molecular scientific achievements. It was Bennett that greenlit the Million Mito project. Bennett’s challenge as a genetic genealogy/business owner was to advance the science that led to products while making enough money to be able to continue advancing the science. It was a fine line, but Max and Bennett navigated those waters quite well.

Apparently, Max, Bennett, and the FamilyTreeDNA customers weren’t the only people who believe that.

In January 2021, myDNA acquired and merged with FamilyTreeDNA. Max and Bennett remain involved as board members.

Dr.Lior Rauchberger, CEO of myDNA which includes FamilyTreeDNA

Dr. Lior Rauchberger, the CEO of the merged enterprise believes in the power of genetics, including genetic genealogy, and is continuing to make investments in FamilyTreeDNA products – including new features. There have already been improvements in 2021 and in the presentation by Katy Rowe, the Product Manager for the FamilyTreeDNA products, she explains what is coming this year.

I hope you enjoyed this retrospective on the past 22 years and are looking forward to crossing new frontiers, and breaking down those brick walls, in the coming decades.

Sneak Peek at FamilyTreeDNA – New Features and Upcoming Releases

You can watch Katy Rowe’s Sneak Peek video about what’s coming, here.

Of course, while other companies need to split their focus between traditional genealogy research records and DNA, FamilyTreeDNA does not. Their only focus is genetics. They plan to make advances in every aspect of their products.

FamilyTreeDNA announced a new Help Center which you can access, here. I found lots of short videos and other helpful items. I had no idea it existed.

In 2021, customers began being able to order a combined Family Finder and myDNA test to provide insights into genealogy along with health and wellness

Wellness includes nutrition and fitness insights.

Existing customers either are or will be able to order the myDNA upgrade to their existing test. The ability to upgrade is being rolled out by groups. I haven’t had my turn yet, but when I do, I’ll test and let you know what I think. Trust me, I’m not terribly interested in how many squats I can do anymore, because I already know that number is zero, but I am very interested in nutrition and diet. I’d like to stay healthy enough to research my ancestors for a long time to come.

FamilyTreeDNA announced that over 72,000 men have taken the Big Y test which has resulted in the Y DNA tree of mankind surpassing 50,000 branches.

This is utterly amazing when you consider how far we’ve come since 2002. This also means that a very high number of men, paired with at least one other man, actually form a new branch on the Y haplotree.

The “age” of tester’s Y DNA haplogroups is now often within the 500-year range – clearly genealogical in nature. Furthermore, many leaf-tip haplogroups as defined by the Big Y SNPs are much closer than that and can differentiate between branches of a known family. The Big Y-700 is now the go-to test for Y DNA and genealogy.

Of course, all these new branches necessitate new maps and haplogroup information. These will be released shortly and will provide users with the ability to see the paths together, which is the view you see here, or track individual lines. The same is true for mitochondrial DNA as well.

Y DNA tree branch ages will be forthcoming soon too. I think this is the #1 most requested feature.

On the Mitochondrial DNA side of the house, the Million Mito project has led to a significant rewrite of the MitoTree. As you know, I’m a Million Mito team member.

Here’s Dr. Paul Maier’s branch, for example. You can see that in the current version of the Phylotree, there is one blue branch and lots of “child” branches beneath that. Of course, when we’re measuring the tree from “Eve,” the end tip leaf branches look small, but it’s there that our genealogy resides.

In the new version, yet to be released, there is much more granularity in the branches of U5a2b2a.

To put this another way, in today’s tree, haplogroup U5a2b2a is about 5,000 years old, but the newly defined branches bring the formation of Paul’s (new) haplogroup into the range of about 500 years. Similar in nature to the Y DNA tree and significantly more useful for genealogical purposes. If you have not taken a mitochondrial DNA full sequence test, please order one now. Maybe your DNA will help define a new branch on the tree plus reveal new information about your genealogy.

Stay tuned on this one. You know the Million Mito Project is near and dear to my heart.

2022 will also see much-needed improvements in the tree structure and user experience, as well as the matches pages.

There are a lot of exciting things on FamilyTreeDNA’s plate and I’m excited to see these new features and functions roll out over the next few months.

Just the Beginning

The three days of RootsTech 2022 may be over, but the content isn’t.

In fact, it’s just the beginning of being able to access valuable information at your convenience. The vendor booths will remain in the Expo Hall until RootsTech 2023, so for a full year, plus the individual instructor’s sessions will remain available for three years.

In a few days, after I take a break, I’ll publish a full list of DNA sessions, along with links for your convenience.

Thank You Shout Outs

I want to say a HUGE thank you to RootsTech for hosting the conference and making it free. I specifically want to express my gratitude to the many, many people working diligently behind the scenes during the last year, and frantically during the past three days.

Another huge thank you to the speakers and vendors whose efforts provide the content for the conference.

And special thanks to you for loving genealogy, taking your time to watch and learn, and for reading this blog.

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DNA from 459 Ancient British Isles Burials Reveals Relationships – Does Yours Match?

In December 2021, two major papers were released that focused on the ancient DNA of burials from Great Britain. The paper, A high-resolution picture of kinship practices in an Early Neolithic tomb by Fowler et al provided a genetic analysis of 35 individuals from a Cotswold Neolithic burial who were found to be a multi-generational family unit. In Large-scale migration into Britain during the Middle to Late Bronze Age by Patterson et, the authors generated genome-wide data for 793 ancient burials from the British Isles and continental Europe to determine who settled Great Britain, from where, and when.

Of course, the very first thing genealogists want to know is, “Am I related?”

If we are related, it’s far too distant for the reach of autosomal DNA, but Y DNA and mitochondrial DNA might just be very interesting. If you haven’t yet tested your mother’s line mitochondrial DNA for males and females both, and paternal line Y DNA for males only, you’re in luck because you can purchase those tests here.

These two papers combined provide a significant window into the past in Great Britain; England, Scotland, Wales, and nearby islands.

First, let’s take a look at the Cotswold region.

The Cotswolds

Ancient DNA was retrieved from a cairn burial in the Cotswolds, a hilly region of Southwest England.

By Saffron Blaze – Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=15675403

Even today, the paused-in-time stone houses, fences, and ancient gardens harken back to earlier times.

By Peter K Burian – Own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=70384620

Stunningly beautiful and historically important, the Cotswolds is a protected landscape that includes Neolithic burial chambers (3950-2450 BCE), Bronze and Iron Age forts, Roman villas, and eventually, the Celtic pathway known as Fosse Way.

The Hazelton North Long-Tomb Burial Site

The Fowler paper explores the kinship practices and relationships between the Cotswolds burials.

Click to enlarge images

The North Hazelton site was endangered due to repeated plowing in a farmer’s field. Excavation of the tomb occurred in 1981. A book was published in 1990 with a pdf file available at that link. The photo from 1979 on page 3 shows that the burial cairn only looks to be a slight rise in the field.

You can see on the map below from the UK Megalithic site map that there are many other locations in close proximity to the Hazelton North site, some with similarly arranged burials.

The paper’s authors state that there are 100 long cairns within 50 km of Hazelton North, and one only 80 meters away. Excavation in those tombs, along with archaeological evaluation would be needed to determine the ages of the cairns, if burial practices were the same or similar, and if any of the individuals were related to each other or the individuals in the North Hazelton cairn. In other words, were these separate cemeteries of an extended family, or disconnected burial grounds of different groups of people over time.

While the North Hazelton site no longer exists, as it was entirely excavated, on the same page, you can see photos before excavation, along with the main chamber which now resides in the Corinium Museum in Cirencester, just a few kilometers away.

The Fowler team analyzed 35 individuals who lived about 5,700 years ago, at least 100 years after cattle and cereal cultivation was introduced to Britain along with the construction of megalithic monuments. Stonehenge, the most well-known megalith, is located about 90 miles away and is estimated to be about 5,100 years old. The burials from Stonehenge indicate that they were primarily Early European Farmers (EEF) from Anatolia who first moved to Iberia, then on to Britain.

The remains analyzed in this paper were excavated from the Hazelton North Megalithic long-cairn type tomb.

The tomb was built between 5,695 and 5,650 years ago, with the stonework of the north passage collapsing and sealing off the north chamber between 5,660 and 5,630 years ago. All burials stopped in this location about 5,620 years ago, so the site was only in use for about 80 years.

The tomb seems to have been built with multiple passages in anticipation of planned burials by genealogical association. The arrangement of burials was determined by kinship, at least until the passage wall of the North chamber collapsed. The southern and northern chambers each housed two females’ descendants, respectively. While the male progenitor was significant in that this entire tomb was clearly his family tomb, the arrangement of the burials within the chambers suggests that the women were socially significant in the community, and to their families as well.

Osteological analysis reveals at least 41 individuals, 22 of whom were adults. Strontium isotope analysis indicates that most of the individuals had spent time in their childhood at least 40 km away. Authors of a 2015 paper interpret this to mean that the population as a whole was not sedentary, meaning that they may have moved with their livestock from place to place, perhaps based on seasons. Of course, this also calls into question what happened if an individual died while the group was not in the location of the burial cairn.

Of those individuals, 27 people were part of a 5-generation family with many interrelationships.

Of the 15 intergenerational genetic transmissions, all were through men, meaning every third, fourth or fifth generation individual was connected to the original patriarch through only males, suggesting that patrilineal descent determined who was buried in a Neolithic tomb. This also tells us that patrilineal social practices were persistent.

26 of 35 people with genetic data were male. Male burials in other Cotswold tombs outnumber females 1.6 to 1. The remains of some women must have been treated differently.

No adult lineage daughters were present in the tomb, although two infant daughters were, suggesting that adult daughters were out-married, outside of either the community or this specific family lineage. They would have been buried in their husband’s tomb, just as these women were buried here.

The male progenitor reproduced with 4 females, producing 14 adult sons who were buried in the tomb. All four females were buried in the tomb, in two chambers, suggesting that women, at least high-status women were buried with their partners and not in their father’s tomb.

The lineages of two of those women were buried in the same half of the tomb over all generations, suggesting maternal lineages were socially important.

The burials included four men who did not descend from the male progenitors of the clan lineage but DID descend from women who also had children with the progenitors. The authors state that this suggests that the progenitor men adopted the four children of their mates into their lineage, but it also raises the possibility that the progenitor men were not aware that those four men were not their descendants.

Multiple reproductive partners of men were not related to each other, but multiple reproductive partners of women were.

Eight individuals found within the tomb were not closely related to the main lineages. This could mean that they were partners of men who did not reproduce, or who had only adult daughters. It could also mean they were socially important, but not biologically related to either each other nor the tomb’s family members whose DNA was sampled.

Of those who are related, inbreeding had been avoided meaning the parents of individuals were not related to each other based on runs of homozygosity (ROH).

Some of the remains from the north chamber had been gnawed by scavengers, apparently before burial, and three cremations were buried at the entrance including an infant, a child, and an adult. This might answer the question of what happened if someone died while the group was away from the burial site.

Individuals in the north tomb exhibited osteoarthritis typical of other burials in southern England, and signs of nutritional stress in childhood.

The south chamber burials were more co-mingled and dispersed among neighboring compartments.

In the Guardian article, World’s oldest family tree revealed in 5,700-year-old Cotswolds tomb, a genetic pedigree chart was drawn based on the burials, their relationship to each other, and burial locations.

As discussed in this PNAS paper, Megalithic tombs in western and northern Neolithic Europe were linked to a kindred society, other Neolithic tomb burials in Europe were also reflective of a kinship system.

The question remains, where did the Cotswold settlers come from? Who were they descended from and related to? The second paper provides insights to that question.

Who Migrated into Britain, and When?

Patterson et al tell us that their DNA analysis of 793 individuals increased the data from the Middle (1550-1150 BCE) to Late Bronze (1150-750 BCE) and Iron Age (70-BCE-43CE) in Britain by 12-fold, and from Western and Central Europe by 3.5 times.

They also reveal that present-day people from England and Wales carry more ancestry derived from Early European Farmers than people from the Early Bronze Age.

The DNA contributed from Early European Farmers (EEF) increased over time in people in the southern portion of Britain and Wales, which includes the Cotswold region, but did not increase in northern Britain (Scotland,) nor in Kent. Specifically, from 31% in the Early Bronze Age to 34% in the Middle Bronze Age to 35% in the Late Bronze Age to 38% in the Iron Age.

While the EEF DNA increased over time in the Southwest area of Britain, it decreased in other regions. This means that the increase could not be explained by migration from northern continental Europe in the medieval period because those early migrants carried even less Early European Farmer ancestry than the inhabitants of Southwest Britain. Therefore, if those two populations had admixed, the results would be progressively lower EEF in Southwest Britain, not higher.

To fully evaluate this data, the team sequenced earlier samples from both Britain and mainland Europe in addition to the Cotswold burials, targeting 1.2 million SNP locations.

In addition to DNA sequencing, they also utilized radiocarbon dating to confirm the age of the remains.

Results for low-coverage individuals, meaning those with less than 30,000 SNPs scanned at least once, were removed from the data set.

123 individuals were identified as related to each other from 48 families within the third degree. Third-degree relatives share approximately 12.5% of their DNA and would include first cousins, great-grandparents/children, granduncles/aunts, half uncles/aunts/nieces/nephews.

Lactase persistence, the ability to digest the lactose in milk was significantly higher in this population than in either the rest of Britain or Central and Western Europe by a factor of 5 or greater.

The DNA of the Cotswold burial groups and others found from this early timeframe in Southwest Britain and Wales is most similar to ancient burials from France.

A Eupedia megalithic culture page shows a map of various major megalithic sites in both Europe and the British Isles.

Based on charts in Figure 4 of the paper, the location in Europe with the highest percentage of EEF about 4300 years ago (2300 BCE) was the Iberian Peninsula – Spain and Portugal, a location that neighbors France. Lactase persistence began increasing about that time and dramatically rose about 3500 years ago (1500 BCE.)

Y DNA haplogroup R-L21/M529 went from 0% in the Neolithic era (3950-2450 BCE,) or about 5950-4450 years ago) in Britain to 90% in all of Britain in the Early Bronze Era (2450-1550 BCE or 4450-3550 years ago), then dropped slowly to about 70% in the Iron Age in Western England and Wales, then 50% in western Britain and Wales and 20% in Central and Eastern Britain in the Modern Era.

You can read more about this research in this Phys.org article: Geneticists’ new research on ancient Britain contains insights on language, ancestry, kinship, milk, and more about Megalithic burials in France in this Smithsonian Magazine article: Europe’s Megalithic Monuments Originated in France and Spread by Sea Routes, new Study Suggests.

Are You Connected?

The paper authors made the resequenced Y DNA and mitochondrial DNA information available for analysis.

Of course, we all want to know if we are connected with these people, especially if our families have origins in the British Isles.

The R&D team at FamilyTreeDNA downloaded the Y DNA and mitochondrial DNA sequences and linked them to mapped locations. They also correlated samples to Y DNA and mitochondrial DNA haplogroups and linked them to their respective public trees here and here. The Y DNA sometimes contained additional SNP information which allowed a more granular haplogroup to be assigned.

I want to specifically thank Goran Runfeldt, head of R&D, for making this valuable information available and useful for genealogists by downloading, reformatting, and mapping the data, and Michael Sager, phylogeneticist in the FamilyTreeDNA lab, for reanalyzing the Y DNA results and refining them beyond the papers.

Now, let’s get to the best part.

The Map

This map shows the locations of 459 ancient British Isles burials included in the papers, both in the Cotswolds and throughout the rest of Great Britain.

There are significantly more mitochondrial DNA haplogroups represented than Y DNA. Of course, everyone, males and females both have mitochondrial DNA, so everyone can test, but only males carry Y DNA.

The next map shows the distribution of the base mitochondrial haplogroups.

  • H=light green (181 samples)
  • U=rust (70 samples)
  • K=burgundy (68 samples)
  • J=yellow (46 samples)
  • T=dark green (43 samples)
  • V=grey (16 samples)
  • X=dark teal (9 samples)
  • I=orange (6 samples)
  • W=purple (6 samples)
  • N=brown (2 samples)

The most common mitochondrial haplogroup found is H which is unsurprising given that H is the most common haplogroup in Europe as well.

It’s interesting to note that there is no clear haplogroup distribution pattern for either Y DNA or mitochondrial  DNA, with the exception of the North Hazelton burials themselves as outlined in the paper.

There were only three ancient major Y DNA haplogroups discovered.

  • R=green (179 samples)
  • I=gold (50 samples)
  • G=blue (5 samples)

225 total samples were female and had no Y chromosome. A few male Y chromosomes were not recoverable.

Of course, some samples on the maps fall directly beneath other samples, so it’s difficult to discern multiple samples from the same location.

For that, and for more granular haplogroups, we need to refer to the data itself.

How to Use the Data

Each sample is identified by:

  • A sample ID from the papers
  • Sex
  • Location with a google map link.
  • Age calibrated to BCE, before current era, which means roughly how many years before about the year 1 that someone lived. To determine approximately how long ago one of these people lived, add 2000 to the BCE date. For example, 3500 BCE equates to 5500 years ago.
  • Y DNA haplogroup for male samples where recoverable, linked to FamilyTreeDNA’s public Y DNA haplotree.
  • Mitochondrial DNA haplogroup for all but 2 samples where mitochondrial results were not recoverable, linked to FamilyTreeDNA’s public mitochondrial DNA haplotree.

If you have tested your full sequence mitochondrial DNA, you can use the browser search function (ctrl+F) on a PC to search for your haplogroup. For example. Searching for haplogroup H61 produces 5 results. Click on the sample locations to view where they were found. Are they in close proximity to each other? In the same burial?

Four were found at the same location in the Channel Islands, and one in Kent. Where is your ancestor from?

For Y DNA, you can search for your haplogroup, but if you’ve taken the Big Y test and don’t find your specific haplogroup, you might want to use the Y DNA tree to search for successive upstream haplogroups to see where your closest ancient match might be found. Of course, if you’re haplogroup G, it’s pretty easy to just take a look without searching for each individual haplogroup. Just search for “G-“.

For each sample, be sure to click on the haplogroup name itself to view its location on the tree and where else in the world this haplogroup is found. Let’s look at a couple of examples.

Sample: I26628 (Female)
Location: Channel Islands, Alderney, Longis Common
Age: 756-416 calBCE
mtDNA: H61

Mitochondrial haplogroup H61, above, is fairly rare and currently found sparsely in several countries including England, Germany, Hungary, Belarus, Ireland, Netherlands, the UK, and France. The flags indicate the location of FamilyTreeDNA testers’ earliest known ancestor of their mitochondrial, meaning direct matrilineal, line.

Click on the haplogroup link to view the results in the Y or mtDNA trees.

Next, let’s look at a Y DNA sample.

Sample: I16427 (Male)
Location: Channel Islands, Guernsey, Vale, Le Déhus
Age: 4234-3979 calBCE
Y-DNA: I-M423
mtDNA: X2b-T226C

Haplogroup I-M423 itself is found most frequently in Germany, Poland, Ukraine, Scotland and Ireland, but note that it also has 648 downstream branches defined. You may match I-M423 by virtue of belonging to a downstream branch.

Do you match any of these ancient samples, and where were your ancestors from?

Sample: I26630 (Male)
Location: Channel Islands, Alderney, Longis Common
Age: 749-403 calBCE
mtDNA: H61

Sample: I16430 (Female)
Location: Channel Islands, Alderney, Longis Common
Age: 337-52 calBCE
mtDNA: H61

Sample: I16505 (Female)
Location: Channel Islands, Alderney, Longis Common
Age: 174-45 calBCE
mtDNA: H61

Sample: I26629 (Female)
Location: Channel Islands, Alderney, Longis Common
Age: 170 calBCE – 90 calCE
mtDNA: U5a1b1

Sample: I16437 (Female)
Location: Channel Islands, Guernsey, Vale, Le Déhus
Age: 4241-4050 calBCE
mtDNA: K1b1a1

Sample: I16444 (Male)
Location: Channel Islands, Guernsey, Vale, Le Déhus
Age: 4228-3968 calBCE
Y-DNA: I-FT376000
mtDNA: J1c1b1

Sample: I16429 (Male)
Location: Channel Islands, Guernsey, Vale, Le Déhus
Age: 3088-2914 calBCE
mtDNA: K1

Sample: I16425 (Female)
Location: Channel Islands, Guernsey, Vale, Le Déhus
Age: 3083-2912 calBCE
mtDNA: K1a4a1

Sample: I16438 (Male)
Location: Channel Islands, Guernsey, Vale, Le Déhus
Age: 2567-2301 calBCE
Y-DNA: I-L623
mtDNA: J1c8

Sample: I16436 (Male)
Location: Channel Islands, Herm, The Common
Age: 3954-3773 calBCE
Y-DNA: I-CTS7213
mtDNA: HV

Sample: I16435 (Male)
Location: Channel Islands, Herm, The Common
Age: 3646-3527 calBCE
mtDNA: H

Sample: I16597 (Male)
Location: England, Bedfordshire, Broom Quarry
Age: 404-209 calBCE
Y-DNA: R-DF49
mtDNA: H1-C16355T

Sample: I21293 (Female)
Location: England, Bedfordshire, Broom Quarry
Age: 425-200 BCE
mtDNA: J1c1b

Sample: I11151 (Male)
Location: England, Bedfordshire, Broom Quarry
Age: 379-197 calBCE
Y-DNA: R-FT44983
mtDNA: K1a-T195C!

Sample: I11150 (Male)
Location: England, Bedfordshire, Broom Quarry
Age: 381-197 calBCE
Y-DNA: R-FT335377
mtDNA: H15a1

Sample: I19047 (Male)
Location: England, Cambridgeshire, Babraham Research Campus (ARC05), ARES site
Age: 1-50 CE
Y-DNA: R-M269
mtDNA: H2a

Sample: I19045 (Male)
Location: England, Cambridgeshire, Marshall’s Jaguar Land Rover New Showroom (JLU15)
Age: 388-206 calBCE
Y-DNA: G-S23438
mtDNA: U4a2

Sample: I19046 (Male)
Location: England, Cambridgeshire, Marshall’s Jaguar Land Rover New Showroom (JLU15)
Age: 383-197 calBCE
Y-DNA: R-P312
mtDNA: H1t

Sample: I19044 (Male)
Location: England, Cambridgeshire, Marshall’s Jaguar Land Rover New Showroom (JLU15)
Age: 381-199 calBCE
Y-DNA: R-FT50512
mtDNA: K1a-T195C!

Sample: I11152 (Male)
Location: England, Cambridgeshire, Over
Age: 355-59 calBCE
Y-DNA: G-Z16775
mtDNA: U3a1

Sample: I11149 (Male)
Location: England, Cambridgeshire, Teversham (Marshall’s) Evaluation
Age: 733-397 calBCE
Y-DNA: R-Z156
mtDNA: V

Sample: I11154 (Female)
Location: England, Cambridgeshire, Trumpington Meadows
Age: 743-404 calBCE
mtDNA: H5a1

Sample: I13729 (Female)
Location: England, Cambridgeshire, Trumpington Meadows
Age: 512-236 calBCE
mtDNA: H1ag1

Sample: I11153 (Male)
Location: England, Cambridgeshire, Trumpington Meadows
Age: 405-209 calBCE
Y-DNA: R-FGC33066
mtDNA: H3b

Sample: I13727 (Female)
Location: England, Cambridgeshire, Trumpington Meadows
Age: 389-208 calBCE
mtDNA: T1a1

Sample: I13728 (Male)
Location: England, Cambridgeshire, Trumpington Meadows
Age: 381-179 calBCE
Y-DNA: R-P312
mtDNA: T2a1a

Sample: I13687 (Female)
Location: England, Cambridgeshire, Trumpington Meadows
Age: 368-173 calBCE
mtDNA: W1c

Sample: I11156 (Male)
Location: England, Cambridgeshire, Whittlesey, Bradley Fen
Age: 382-200 calBCE
Y-DNA: R-CTS8704
mtDNA: J1c3

Sample: I11997 (Male)
Location: England, Cambridgeshire, Whittlesey, Bradley Fen
Age: 377-197 calBCE
Y-DNA: R-FGC36434
mtDNA: X2b-T226C

Sample: I16620 (Female)
Location: England, Co. Durham, Hartlepool, Catcote
Age: 340 BCE – 6 CE
mtDNA: H1bs

Sample: I12790 (Female)
Location: England, Cornwall, Newquay, Tregunnel
Age: 400-100 BCE
mtDNA: H2a1

Sample: I12793 (Male)
Location: England, Cornwall, Newquay, Tregunnel
Age: 400-100 BCE
Y-DNA: R-L21
mtDNA: H2a1

Sample: I12792 (Female)
Location: England, Cornwall, Newquay, Tregunnel
Age: 400-100 BCE
mtDNA: H2a1

Sample: I16387 (Male)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
Y-DNA: R-P312
mtDNA: N/A

Sample: I16456 (Female)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
mtDNA: T1a1’3

Sample: I16455 (Male)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
Y-DNA: R-Z290
mtDNA: T1

Sample: I16386 (Female)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
mtDNA: T1a1

Sample: I16458 (Male)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
Y-DNA: R-L21
mtDNA: T2c1d-T152C!

Sample: I16457 (Female)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
mtDNA: T1a1

Sample: I16450 (Male)
Location: England, Cornwall, Newquay, Trethellan Farm
Age: 300 BCE – 100 CE
Y-DNA: R-FT32396
mtDNA: T1a1

Sample: I16424 (Female)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 2285-2036 calBCE
mtDNA: R1b

Sample: I6769 (Male)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 754-416 calBCE
Y-DNA: R-BY168376
mtDNA: H6a1b2

Sample: I16380 (Male)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
Y-DNA: R-ZP298
mtDNA: U4b1a1a1

Sample: I16388 (Female)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
mtDNA: J1c1

Sample: I16440 (Male)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
Y-DNA: R-P312
mtDNA: T2c1d-T152C!

Sample: I16441 (Female)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
mtDNA: J1c2e

Sample: I16442 (Female)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
mtDNA: U4b1a1a1

Sample: I16439 (Female)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
mtDNA: T2c1d-T152C!

Sample: I12772 (Male)
Location: England, Cornwall, Padstow, St. Merryn, Harlyn Bay
Age: 800 BCE – 43 CE
Y-DNA: G-CTS2230
mtDNA: T2c1d-T152C!

Sample: I16453 (Male)
Location: England, Cornwall, St. Mawes, Tregear Vean
Age: 800-1 BCE
Y-DNA: I-M253
mtDNA: U5a2a1

Sample: I16454 (Male)
Location: England, Cornwall, St. Merryn, Constantine Island
Age: 1381-1056 calBCE
Y-DNA: R-Z290
mtDNA: U5b2b2

Sample: I20997 (Male)
Location: England, Cumbria, Ulverston, Birkrigg Common
Age: 2450-1800 BCE
Y-DNA: R-A286
mtDNA: X2b4a

Sample: I12776 (Female)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 1918-1750 calBCE
mtDNA: U4a2c

Sample: I12774 (Male)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 758-416 calBCE
Y-DNA: R-P312
mtDNA: H10b

Sample: I12771 (Male)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 513-210 calBCE
Y-DNA: R-FT5780
mtDNA: U5b2a2a

Sample: I12778 (Male)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 381-203 calBCE
Y-DNA: R-DF5
mtDNA: H4a1a2

Sample: I3014 (Female)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 377-177 calBCE
mtDNA: H

Sample: I12775 (Male)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 361-177 calBCE
Y-DNA: R-BY9405
mtDNA: U5a1b1e

Sample: I12770 (Female)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 390-171 calBCE
mtDNA: H3b1b1

Sample: I12779 (Female)
Location: England, Derbyshire, Brassington, Carsington Pasture Cave
Age: 370-197 calBCE
mtDNA: T2b4c

Sample: I20620 (Female)
Location: England, Derbyshire, Fin Cop
Age: 382-204 calBCE
mtDNA: T2a1b1

Sample: I20627 (Female)
Location: England, Derbyshire, Fin Cop
Age: 376-203 calBCE
mtDNA: V2b

Sample: I20623 (Female)
Location: England, Derbyshire, Fin Cop
Age: 400-150 BCE
mtDNA: V2b

Sample: I20624 (Male)
Location: England, Derbyshire, Fin Cop
Age: 356-108 calBCE
Y-DNA: R-M269
mtDNA: U2e1a1

Sample: I20622 (Male)
Location: England, Derbyshire, Fin Cop
Age: 357-60 calBCE
Y-DNA: I-Y3713
mtDNA: T2c1d1

Sample: I20634 (Male)
Location: England, Derbyshire, Fin Cop
Age: 400-50 BCE
Y-DNA: R-M269
mtDNA: K2b1a1a

Sample: I20630 (Male)
Location: England, Derbyshire, Fin Cop
Age: 400-50 BCE
Y-DNA: R-L21
mtDNA: H1au1b

Sample: I20632 (Male)
Location: England, Derbyshire, Fin Cop
Age: 400-50 BCE
Y-DNA: R-P310
mtDNA: V2b

Sample: I20621 (Female)
Location: England, Derbyshire, Fin Cop
Age: 400-50 BCE
mtDNA: T2c1d1

Sample: I20631 (Female)
Location: England, Derbyshire, Fin Cop
Age: 400-50 BCE
mtDNA: V2b

Sample: I20628 (Male)
Location: England, Derbyshire, Fin Cop
Age: 351-52 calBCE
Y-DNA: R-DF13
mtDNA: I2a

Sample: I20626 (Male)
Location: England, Derbyshire, Fin Cop
Age: 346-53 calBCE
Y-DNA: I-P222
mtDNA: H7b

Sample: I20625 (Male)
Location: England, Derbyshire, Fin Cop
Age: 343-49 calBCE
Y-DNA: R-P310
mtDNA: T1a1

Sample: I27382 (Male)
Location: England, Dorset, Long Bredy, Bottle Knap
Age: 774-540 calBCE
Y-DNA: R-BY116228
mtDNA: H1

Sample: I27383 (Female)
Location: England, Dorset, Long Bredy, Bottle Knap
Age: 750-411 calBCE
mtDNA: U4c1

Sample: I27381 (Female)
Location: England, Dorset, Long Bredy, Bottle Knap
Age: 748-406 calBCE
mtDNA: U4c1

Sample: I20615 (Female)
Location: England, Dorset, Worth Matravers, Football Field
Age: 100 BCE – 50 CE
mtDNA: H1i

Sample: I22065 (Male)
Location: England, East Riding of Yorkshire, Burstwick
Age: 351-55 calBCE
Y-DNA: R-P312
mtDNA: H

Sample: I22052 (Female)
Location: England, East Riding of Yorkshire, East Coast Pipeline (field 16)
Age: 344-52 calBCE
mtDNA: U2e2a1a

Sample: I22060 (Male)
Location: England, East Riding of Yorkshire, East Coast Pipeline (field 9)
Age: 343-1 calBCE
Y-DNA: R-BY154824
mtDNA: H4a1a3a

Sample: I0527 (Female)
Location: England, East Riding of Yorkshire, East Riding, North Ferriby, Melton Quarry
Age: 400-100 BCE
mtDNA: U2e1

Sample: I0525 (Female)
Location: England, East Riding of Yorkshire, Melton
Age: 100 BCE – 50 CE
mtDNA: U2e1e

Sample: I7629 (Male)
Location: England, East Riding of Yorkshire, North Ferriby, Melton Quarry
Age: 1201-933 calBCE
Y-DNA: R-DF13
mtDNA: H17

Sample: I5503 (Female)
Location: England, East Riding of Yorkshire, Nunburnholme Wold
Age: 334-42 calBCE
mtDNA: U5b1c2

Sample: I5502 (Male)
Location: England, East Riding of Yorkshire, Nunburnholme Wold
Age: 196-4 calBCE
Y-DNA: R-FT96564
mtDNA: H3

Sample: I11033 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 717-395 calBCE
mtDNA: H2a3b

Sample: I14100 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 409-229 calBCE
Y-DNA: R-DF13
mtDNA: J1c9

Sample: I12412 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 387-205 calBCE
mtDNA: K1c1a

Sample: I5507 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 387-206 calBCE
mtDNA: H2a3b

Sample: I5506 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 358-111 calBCE
mtDNA: K1c1a

Sample: I5504 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: T1a1

Sample: I5505 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-L21
mtDNA: V16

Sample: I14103 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H53

Sample: I5510 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: K1c1a

Sample: I13755 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I5509 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-PH4760
mtDNA: K1c1a

Sample: I13758 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-L2
mtDNA: H2a3b

Sample: I14107 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-CTS6919
mtDNA: K1c1a

Sample: I13760 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-DF13
mtDNA: H2a3b

Sample: I13751 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I13754 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-P312
mtDNA: U5b2b3

Sample: I13757 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: T2c1d1a

Sample: I13756 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: K1c1a

Sample: I13753 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-Z251
mtDNA: H2a3b

Sample: I14099 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I14101 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I14105 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-P312
mtDNA: H2a3b

Sample: I14102 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-FT84170
mtDNA: K1c1a

Sample: I14108 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: V2a

Sample: I14104 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-DF13
mtDNA: H

Sample: I13759 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-BY3865
mtDNA: H2a3b

Sample: I11034 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I12411 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I12415 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: J1c9

Sample: I12413 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-BY50764
mtDNA: H2a3b

Sample: I12414 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
mtDNA: H2a3b

Sample: I5508 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-BY11863
mtDNA: J1c9

Sample: I5511 (Male)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 400-50 BCE
Y-DNA: R-DF63
mtDNA: J1c9

Sample: I13752 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 346-53 calBCE
mtDNA: J1c9

Sample: I14106 (Female)
Location: England, East Riding of Yorkshire, Pocklington (Burnby Lane)
Age: 176 calBCE – 6 calCE
mtDNA: K1c1a

Sample: I18606 (Male)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 10)
Age: 1919-1742 calBCE
Y-DNA: R-DF13
mtDNA: K1b1a1

Sample: I19220 (Female)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 10)
Age: 1894-1695 calBCE
mtDNA: H3g1

Sample: I14326 (Female)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 13)
Age: 3074-2892 calBCE
mtDNA: H1c

Sample: I22056 (Female)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 16)
Age: 391-201 calBCE
mtDNA: H4a1a3a

Sample: I22055 (Female)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 16)
Age: 391-201 calBCE
mtDNA: K1b1a1c1

Sample: I14327 (Male)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 16)
Age: 340-47 calBCE
Y-DNA: R-BY41416
mtDNA: H5

Sample: I22064 (Female)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 16)
Age: 105 calBCE – 64 calCE
mtDNA: H4a1a3a

Sample: I22057 (Female)
Location: England, East Riding of Yorkshire, Thornholme, East Coast Pipeline (field 16)
Age: 104 calBCE – 65 calCE
mtDNA: H2a1k

Sample: I22062 (Male)
Location: England, East Riding of Yorkshire, Thornholme, Town Pasture
Age: 50 calBCE – 116 calCE
Y-DNA: R-BY23382
mtDNA: K1a-T195C!

Sample: I12931 (Male)
Location: England, Gloucestershire, Bishop’s Cleeve, Cleevelands
Age: 50-200 CE
Y-DNA: I-L160
mtDNA: H6a2

Sample: I12927 (Male)
Location: England, Gloucestershire, Bishop’s Cleeve, Cleevelands
Age: 50-200 CE
Y-DNA: R-PR1289
mtDNA: U5b3b1

Sample: I12932 (Female)
Location: England, Gloucestershire, Bishop’s Cleeve, Cleevelands
Age: 50-200 CE
mtDNA: H1bs

Sample: I12791 (Male)
Location: England, Gloucestershire, Bourton-on-the-water, Greystones Farm
Age: 200-1 BCE
Y-DNA: I-BY17900
mtDNA: H1e1a

Sample: I12785 (Male)
Location: England, Gloucestershire, Bourton-on-the-water, Greystones Farm
Age: 200-1 BCE
Y-DNA: R-DF21
mtDNA: J1c1b2

Sample: I12926 (Male)
Location: England, Gloucestershire, Fairford, Saxon Way
Age: 400-100 BCE
Y-DNA: R-L21
mtDNA: H2a2a2

Sample: I21392 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: 3710–3630 calBCE
Y-DNA: I-M284
mtDNA: J2b1a

Sample: I12439 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
Y-DNA: I-Y3709
mtDNA: K1b1a

Sample: I30304 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
Y-DNA: I-L1195
mtDNA: K1b1a

Sample: I13888 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
mtDNA: K1b1a

Sample: I21388 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
Y-DNA: I-Y3709
mtDNA: U8b1b

Sample: I13892 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: 3910–3630 calBCE
Y-DNA: I-Y3709
mtDNA: T2e1

Sample: I30334 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
mtDNA: K1a3a1

Sample: I21390 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: 3950–3630 calBCE
mtDNA: U8b1b

Sample: I30300 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
Y-DNA: I-Y3709
mtDNA: N1b1b

Sample: I13899 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North chamber
Age: N/A
Y-DNA: I-Y3712
mtDNA: U3a1

Sample: I13893 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North entrance
Age: 3650–3380 calBCE
Y-DNA: I-Y3709
mtDNA: K1a4

Sample: I13897 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North entrance
Age: 3500–3340 calBCE
Y-DNA: I-Y3712
mtDNA: V

Sample: I13898 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North entrance
Age: 3700–3530 calBCE
Y-DNA: I-Y3709
mtDNA: K1a3a1

Sample: I12437 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, North entrance
Age: 3790–3510 calBCE
Y-DNA: I-Y3709
mtDNA: K1a3a1

Sample: I21389 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: 3720-3520 calBCE
Y-DNA: I-Y3709
mtDNA: H1

Sample: I30311 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: N/A
Y-DNA: I-Y3709
mtDNA: U5b1-T16189C!-T16192C!

Sample: I21387 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: N/A
mtDNA: K1d

Sample: I12440 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: N/A
Y-DNA: I-Y3709
mtDNA: K2b1

Sample: I30302 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: N/A
mtDNA: K2b1

Sample: I13889 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: N/A
mtDNA: K1b1a1d

Sample: I13896 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber
Age: N/A
mtDNA: J1c1b1

Sample: I21395 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber, south entrance
Age: N/A
Y-DNA: I-Y3709
mtDNA: J1c1b1

Sample: I13891 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber, south passage
Age: N/A
Y-DNA: I-Y3709
mtDNA: U5b1-T16189C!-T16192C!

Sample: I12438 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South chamber, south passage
Age: N/A
Y-DNA: I-L1195
mtDNA: W5

Sample: I30293 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, South entrance
Age: N/A
mtDNA: U5b1-T16189C!

Sample: I30332 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South entrance
Age: N/A
Y-DNA: I-CTS616
mtDNA: N/A

Sample: I21385 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South entrance
Age: N/A
Y-DNA: I-FT344600
mtDNA: K1d

Sample: I13895 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South entrance
Age: N/A
Y-DNA: I-Y3709
mtDNA: U8b1b

Sample: I30301 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South entrance
Age: N/A
Y-DNA: I-Y3712
mtDNA: U5a2d

Sample: I20818 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South entrance, south passage
Age: 3970–3640 calBCE
Y-DNA: I-Y3712
mtDNA: J1c1

Sample: I13890 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South passage
Age: N/A
Y-DNA: I-L1193
mtDNA: T2e1

Sample: I21393 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South passage
Age: N/A
Y-DNA: I-L1195
mtDNA: K1b1a

Sample: I20821 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South passage
Age: N/A
Y-DNA: I-Y3709
mtDNA: H5

Sample: I30299 (Male)
Location: England, Gloucestershire, Hazleton North Long Cairn, South passage
Age: N/A
Y-DNA: I-Y3709
mtDNA: K2b1

Sample: I21391 (Female)
Location: England, Gloucestershire, Hazleton North Long Cairn, Uncertain
Age: N/A
mtDNA: K1b1a1

Sample: I12786 (Male)
Location: England, Gloucestershire, Lechlade-on-Thames, Lechlade Memorial Hall/Skate Park
Age: 2289-2052 calBCE
Y-DNA: R-DF13
mtDNA: J1c2

Sample: I12935 (Male)
Location: England, Gloucestershire, Lechlade-on-Thames, Lechlade Memorial Hall/Skate Park
Age: 2200-1900 BCE
Y-DNA: R-DF21
mtDNA: H1ah2

Sample: I12783 (Male)
Location: England, Gloucestershire, Lechlade-on-Thames, Lechlade Memorial Hall/Skate Park
Age: 783-541 calBCE
Y-DNA: R-DF21
mtDNA: J1c5

Sample: I12787 (Female)
Location: England, Gloucestershire, Lechlade-on-Thames, Lechlade Memorial Hall/Skate Park
Age: 539-387 calBCE
mtDNA: H2a2a1

Sample: I13717 (Female)
Location: England, Hampshire, Barton-Stacey Pipeline
Age: 398-208 calBCE
mtDNA: U5a1a1

Sample: I16611 (Male)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 401-208 calBCE
Y-DNA: R-Z16539
mtDNA: H1c

Sample: I17261 (Male)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 372-175 calBCE
Y-DNA: R-DF63
mtDNA: R0a

Sample: I20987 (Male)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 450-1 BCE
Y-DNA: R-DF63
mtDNA: U5b2b3

Sample: I20985 (Female)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 450-1 BCE
mtDNA: U4a3a

Sample: I17262 (Female)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 357-57 calBCE
mtDNA: T2b

Sample: I20983 (Female)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 450-1 BCE
mtDNA: H3b-G16129A!

Sample: I20986 (Female)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 450-1 BCE
mtDNA: HV0-T195C!

Sample: I20982 (Male)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 450-1 BCE
Y-DNA: R-L20
mtDNA: J1c3

Sample: I20984 (Female)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 450-1 BCE
mtDNA: H1j6

Sample: I16609 (Male)
Location: England, Hampshire, Middle Wallop, Suddern Farm
Age: 341-46 calBCE
mtDNA: J1c2e

Sample: I16612 (Female)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 658-397 calBCE
mtDNA: H3

Sample: I17267 (Female)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 450-100 BCE
mtDNA: V

Sample: I20988 (Male)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 450-100 BCE
Y-DNA: I-Y3713
mtDNA: T2b19

Sample: I17264 (Male)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 450-100 BCE
Y-DNA: R-BY4297
mtDNA: U2e1f1

Sample: I20990 (Female)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 362-171 calBCE
mtDNA: J1c1b1a

Sample: I17266 (Female)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 355-60 calBCE
mtDNA: U5b1b1-T16192C!

Sample: I20989 (Male)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 354-59 calBCE
Y-DNA: R-P312
mtDNA: K1c1

Sample: I16613 (Male)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 351-54 calBCE
mtDNA: J1b1a1

Sample: I17263 (Female)
Location: England, Hampshire, Nether Wallop, Danebury
Age: 346-52 calBCE
mtDNA: J1c1c

Sample: I17260 (Male)
Location: England, Hampshire, Stockbridge, New Buildings
Age: 800-400 BCE
Y-DNA: R-S1051
mtDNA: U5a1a2a

Sample: I17259 (Male)
Location: England, Hampshire, Stockbridge, New Buildings
Age: 725-400 calBCE
Y-DNA: I-S16030
mtDNA: H5a1

Sample: I17258 (Female)
Location: England, Hampshire, Stockbridge, New Buildings
Age: 542-396 calBCE
mtDNA: K1a2

Sample: I19042 (Female)
Location: England, Hampshire, Winnall Down
Age: 715-48 calBCE
mtDNA: T2b33

Sample: I19043 (Female)
Location: England, Hampshire, Winnall Down
Age: 400-100 BCE
mtDNA: J1c1

Sample: I19037 (Female)
Location: England, Hampshire, Winnall Down
Age: 400-100 BCE
mtDNA: J1b1a1b

Sample: I19040 (Female)
Location: England, Hampshire, Winnall Down
Age: 400-100 BCE
mtDNA: H1m

Sample: I14742 (Male)
Location: England, Kent, Cliffs End Farm
Age: 1011-860 calBCE
Y-DNA: R-P312
mtDNA: H1-T16189C!

Sample: I14377 (Female)
Location: England, Kent, Cliffs End Farm
Age: 1014-836 calBCE
mtDNA: U5b1b1d

Sample: I14864 (Female)
Location: England, Kent, Cliffs End Farm
Age: 983-816 calBCE
mtDNA: T2b

Sample: I14862 (Female)
Location: England, Kent, Cliffs End Farm
Age: 982-812 calBCE
mtDNA: H1

Sample: I14865 (Female)
Location: England, Kent, Cliffs End Farm
Age: 967-811 calBCE
mtDNA: H

Sample: I14861 (Male)
Location: England, Kent, Cliffs End Farm
Age: 912-808 calBCE
Y-DNA: R-FGC23071
mtDNA: V

Sample: I14358 (Male)
Location: England, Kent, Cliffs End Farm
Age: 912-807 calBCE
Y-DNA: R-L21
mtDNA: H3

Sample: I14379 (Female)
Location: England, Kent, Cliffs End Farm
Age: 903-807 calBCE
mtDNA: T2c1d-T152C!

Sample: I14745 (Female)
Location: England, Kent, Cliffs End Farm
Age: 900-798 calBCE
mtDNA: X2b

Sample: I14743 (Male)
Location: England, Kent, Cliffs End Farm
Age: 779-524 calBCE
Y-DNA: R-L151
mtDNA: I4a

Sample: I14381 (Female)
Location: England, Kent, Cliffs End Farm
Age: 727-400 calBCE
mtDNA: U5b2b1a1

Sample: I14857 (Female)
Location: England, Kent, Cliffs End Farm
Age: 719-384 calBCE
mtDNA: H3an

Sample: I14747 (Female)
Location: England, Kent, Cliffs End Farm
Age: 514-391 calBCE
mtDNA: H3

Sample: I14378 (Female)
Location: England, Kent, Cliffs End Farm
Age: 400-208 calBCE
mtDNA: I2

Sample: I14858 (Female)
Location: England, Kent, Cliffs End Farm
Age: 396-207 calBCE
mtDNA: J1c1

Sample: I14380 (Male)
Location: England, Kent, Cliffs End Farm
Age: 387-203 calBCE
Y-DNA: R-FTB53005
mtDNA: T2e1

Sample: I14860 (Female)
Location: England, Kent, Cliffs End Farm
Age: 386-198 calBCE
mtDNA: X2b-T226C

Sample: I14859 (Male)
Location: England, Kent, Cliffs End Farm
Age: 377-203 calBCE
Y-DNA: R-P312
mtDNA: H7d3

Sample: I14866 (Male)
Location: England, Kent, Cliffs End Farm
Age: 372-197 calBCE
Y-DNA: I-BY152642
mtDNA: H1at1

Sample: I14863 (Female)
Location: England, Kent, Cliffs End Farm
Age: 360-201 calBCE
mtDNA: U5b1b1-T16192C!

Sample: I13714 (Male)
Location: England, Kent, East Kent Access Road
Age: 1533-1417 calBCE
Y-DNA: R-CTS6919
mtDNA: H1c8

Sample: I19915 (Female)
Location: England, Kent, East Kent Access Road
Age: 1519-1422 calBCE
mtDNA: K1c1

Sample: I19913 (Female)
Location: England, Kent, East Kent Access Road
Age: 1408-1226 calBCE
mtDNA: J1c2e

Sample: I13710 (Male)
Location: England, Kent, East Kent Access Road
Age: 1411-1203 calBCE
Y-DNA: R-DF63
mtDNA: I4a

Sample: I13711 (Male)
Location: England, Kent, East Kent Access Road
Age: 1048-920 calBCE
Y-DNA: R-BY28644
mtDNA: H61

Sample: I13712 (Male)
Location: England, Kent, East Kent Access Road
Age: 1011-916 calBCE
Y-DNA: R-DF13
mtDNA: U5b2b3a

Sample: I13713 (Male)
Location: England, Kent, East Kent Access Road
Age: 1055-837 calBCE
Y-DNA: R-L21
mtDNA: H1c

Sample: I19872 (Female)
Location: England, Kent, East Kent Access Road
Age: 403-209 calBCE
mtDNA: H13a1a1

Sample: I13732 (Male)
Location: England, Kent, East Kent Access Road
Age: 401-208 calBCE
Y-DNA: R-A7835
mtDNA: U5b2c1

Sample: I19873 (Male)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
Y-DNA: R-BY3616
mtDNA: U5b2b

Sample: I13615 (Male)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
Y-DNA: R-DF13
mtDNA: H1c

Sample: I19907 (Female)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
mtDNA: U2e1a1

Sample: I19910 (Female)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
mtDNA: U4a2

Sample: I19911 (Male)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
Y-DNA: R-DF13
mtDNA: K1a4a1

Sample: I19874 (Female)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
mtDNA: H1ax

Sample: I19908 (Female)
Location: England, Kent, East Kent Access Road
Age: 400-200 BCE
mtDNA: K2b1a

Sample: I13731 (Male)
Location: England, Kent, East Kent Access Road
Age: 393-206 calBCE
Y-DNA: R-DF13
mtDNA: U5a1a1g

Sample: I13730 (Male)
Location: England, Kent, East Kent Access Road
Age: 390-202 calBCE
Y-DNA: R-S5668
mtDNA: H1bb

Sample: I19914 (Female)
Location: England, Kent, East Kent Access Road
Age: 387-200 calBCE
mtDNA: H3g1

Sample: I19909 (Male)
Location: England, Kent, East Kent Access Road
Age: 381-197 calBCE
Y-DNA: R-BY9003
mtDNA: T1a1-C152T!!

Sample: I19912 (Female)
Location: England, Kent, East Kent Access Road
Age: 368-173 calBCE
mtDNA: H1bs

Sample: I13616 (Female)
Location: England, Kent, East Kent Access Road
Age: 356-49 calBCE
mtDNA: H1b1-T16362C

Sample: I19870 (Female)
Location: England, Kent, East Kent Access Road
Age: 200-1 BCE
mtDNA: T1a1

Sample: I19869 (Female)
Location: England, Kent, East Kent Access Road
Age: 175 calBCE – 8 calCE
mtDNA: T1a1

Sample: I1774 (Male)
Location: England, Kent, Isle of Sheppey, Neats Court
Age: 1879-1627 calBCE
Y-DNA: R-M269
mtDNA: U4b1a2

Sample: I13716 (Female)
Location: England, Kent, Margetts Pit
Age: 1391-1129 calBCE
mtDNA: H11a

Sample: I13617 (Female)
Location: England, Kent, Margetts Pit
Age: 1214-1052 calBCE
mtDNA: H

Sample: I18599 (Female)
Location: England, Kent, Sittingbourne, Highsted
Age: 43 calBCE – 110 calCE
mtDNA: H

Sample: I3083 (Male)
Location: England, London, River Thames, Putney Foreshore
Age: 387-201 calBCE
Y-DNA: R-P310
mtDNA: R

Sample: I16463 (Male)
Location: England, North Yorkshire, Cockerham, Elbolton Cave
Age: 4000-3500 BCE
Y-DNA: I-L1195
mtDNA: H4a1a2

Sample: I16403 (Male)
Location: England, North Yorkshire, Cockerham, Elbolton Cave
Age: 1600-1350 BCE
Y-DNA: R-DF13
mtDNA: K2a

Sample: I16394 (Male)
Location: England, North Yorkshire, Grassington, 3 Barrow Sites
Age: 2400-1600 BCE
Y-DNA: R-P297
mtDNA: K1c1

Sample: I16395 (Female)
Location: England, North Yorkshire, Grassington, 3 Barrow Sites
Age: 2400-1600 BCE
mtDNA: U5b1

Sample: I16396 (Female)
Location: England, North Yorkshire, Grassington, 3 Barrow Sites
Age: 2400-1600 BCE
mtDNA: K1a4a1

Sample: I16400 (Male)
Location: England, North Yorkshire, Grassington, 3 Barrow Sites
Age: 2400-1500 BCE
Y-DNA: R-Z290
mtDNA: U3a1

Sample: I3035 (Male)
Location: England, North Yorkshire, Ingleborough Hill, Fox Holes Cave
Age: 4000-3500 BCE
Y-DNA: R-A7208
mtDNA: H5a1

Sample: I12936 (Female)
Location: England, North Yorkshire, Raven Scar Cave
Age: 1090-900 BCE
mtDNA: J1c5f

Sample: I16469 (Male)
Location: England, North Yorkshire, Raven Scar Cave
Age: 1090-900 BCE
Y-DNA: R-P312
mtDNA: H3-T152C!

Sample: I16467 (Male)
Location: England, North Yorkshire, Raven Scar Cave
Age: 1090-900 BCE
Y-DNA: R-M269
mtDNA: U5a1g1

Sample: I16459 (Unknown sex)
Location: England, North Yorkshire, Raven Scar Cave
Age: 1090-900 BCE
mtDNA: H

Sample: I19587 (Male)
Location: England, North Yorkshire, Scorton Quarry
Age: 195 calBCE – 7 calCE
Y-DNA: G-L140
mtDNA: K2a

Sample: I14097 (Male)
Location: England, North Yorkshire, Scorton Quarry
Age: 162 calBCE – 26 calCE
Y-DNA: R-P310
mtDNA: H66a1

Sample: I14096 (Male)
Location: England, North Yorkshire, Scorton Quarry
Age: 101 calBCE – 59 calCE
Y-DNA: R-FTA11009
mtDNA: H4a1a2a

Sample: I20583 (Male)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 387-201 calBCE
Y-DNA: R-BY175423
mtDNA: K1a4a1

Sample: I20582 (Female)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 368-165 calBCE
mtDNA: H10

Sample: I21272 (Male)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 400-100 BCE
Y-DNA: R-S5488
mtDNA: V

Sample: I21276 (Female)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 400-100 BCE
mtDNA: K1a4a1

Sample: I21277 (Male)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 400-100 BCE
Y-DNA: R-DF13
mtDNA: K1a4a1

Sample: I21274 (Female)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 400-100 BCE
mtDNA: K1a4a1

Sample: I21275 (Female)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 400-100 BCE
mtDNA: K1a4a1

Sample: I21271 (Female)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 400-100 BCE
mtDNA: W1c

Sample: I20584 (Female)
Location: England, Oxfordshire, Stanton Harcourt, Gravelly Guy
Age: 355-54 calBCE
mtDNA: K1a4a1

Sample: I14808 (Female)
Location: England, Oxfordshire, Thame
Age: 401-209 calBCE
mtDNA: H1

Sample: I14802 (Female)
Location: England, Oxfordshire, Thame
Age: 393-206 calBCE
mtDNA: X2d

Sample: I14807 (Male)
Location: England, Oxfordshire, Thame
Age: 391-204 calBCE
Y-DNA: R-DF49
mtDNA: T1a1

Sample: I14804 (Female)
Location: England, Oxfordshire, Thame
Age: 387-201 calBCE
mtDNA: H1o

Sample: I14806 (Female)
Location: England, Oxfordshire, Thame
Age: 386-198 calBCE
mtDNA: H1bb

Sample: I14800 (Male)
Location: England, Oxfordshire, Thame
Age: 382-197 calBCE
Y-DNA: R-Z253
mtDNA: J2b1

Sample: I14803 (Male)
Location: England, Oxfordshire, Thame
Age: 370-175 calBCE
Y-DNA: R-P312
mtDNA: H2a1

Sample: I14801 (Female)
Location: England, Oxfordshire, Thame
Age: 362-163 calBCE
mtDNA: X2b-T226C

Sample: I14809 (Male)
Location: England, Oxfordshire, Thame
Age: 358-108 calBCE
Y-DNA: R-P312
mtDNA: V7

Sample: I2446 (Female)
Location: England, Oxfordshire, Yarnton
Age: 2454-2139 calBCE
mtDNA: K1b1a1

Sample: I2448 (Male)
Location: England, Oxfordshire, Yarnton
Age: 1500-1000 BCE
Y-DNA: R-DF63
mtDNA: U8a2

Sample: I20585 (Female)
Location: England, Oxfordshire, Yarnton
Age: 800-400 BCE
mtDNA: K1c1

Sample: I21180 (Male)
Location: England, Oxfordshire, Yarnton
Age: 396-209 calBCE
Y-DNA: R-DF13
mtDNA: H7a1

Sample: I19209 (Male)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
mtDNA: H

Sample: I19211 (Male)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
Y-DNA: R-L21
mtDNA: H1

Sample: I20589 (Male)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
Y-DNA: R-Z52
mtDNA: V

Sample: I20586 (Male)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
Y-DNA: R-L21
mtDNA: J2b1a

Sample: I21178 (Female)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
mtDNA: T2b3-C151T

Sample: I21182 (Male)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
Y-DNA: R-BY15941
mtDNA: J1c2

Sample: I21181 (Male)
Location: England, Oxfordshire, Yarnton
Age: 400-200 BCE
Y-DNA: R-DF13
mtDNA: H3

Sample: I20587 (Male)
Location: England, Oxfordshire, Yarnton
Age: 389-208 calBCE
Y-DNA: R-DF63
mtDNA: K1a2a

Sample: I19207 (Male)
Location: England, Oxfordshire, Yarnton
Age: 382-205 calBCE
Y-DNA: R-M269
mtDNA: H

Sample: I21179 (Female)
Location: England, Oxfordshire, Yarnton
Age: 381-201 calBCE
mtDNA: T2b

Sample: I20588 (Male)
Location: England, Oxfordshire, Yarnton
Age: 366-197 calBCE
Y-DNA: G-BY27899
mtDNA: V

Sample: I19210 (Female)
Location: England, Oxfordshire, Yarnton
Age: 355-118 calBCE
mtDNA: H1cg

Sample: I3019 (Male)
Location: England, Somerset, Cheddar, Totty Pot
Age: 4000-2400 BCE
Y-DNA: R-P310
mtDNA: H4a1a-T195C!

Sample: I16591 (Male)
Location: England, Somerset, Christon, Dibbles Farm
Age: 408-232 calBCE
Y-DNA: R-Z290
mtDNA: H13a1a1

Sample: I11148 (Female)
Location: England, Somerset, Christon, Dibbles Farm
Age: 407-211 calBCE
mtDNA: U6d1

Sample: I13685 (Female)
Location: England, Somerset, Christon, Dibbles Farm
Age: 400-208 calBCE
mtDNA: U5a1b1e

Sample: I11147 (Female)
Location: England, Somerset, Christon, Dibbles Farm
Age: 392-204 calBCE
mtDNA: U5a1b1e

Sample: I16592 (Male)
Location: England, Somerset, Christon, Dibbles Farm
Age: 387-199 calBCE
Y-DNA: R-FGC19329
mtDNA: U5a1b1e

Sample: I17014 (Male)
Location: England, Somerset, Christon, Dibbles Farm
Age: 381-179 calBCE
Y-DNA: R-DF63
mtDNA: U5b1b1d

Sample: I17015 (Female)
Location: England, Somerset, Christon, Dibbles Farm
Age: 380-197 calBCE
mtDNA: H2a2a1

Sample: I17016 (Male)
Location: England, Somerset, Christon, Dibbles Farm
Age: 377-178 calBCE
Y-DNA: R-BY3231
mtDNA: U2e1a1

Sample: I17017 (Female)
Location: England, Somerset, Christon, Dibbles Farm
Age: 196 calBCE – 5 calCE
mtDNA: U5b1-T16189C!

Sample: I19653 (Male)
Location: England, Somerset, Ham Hill
Age: 400-200 BCE
Y-DNA: R-L151
mtDNA: H1n6

Sample: I19856 (Female)
Location: England, Somerset, Ham Hill
Age: 400-200 BCE
mtDNA: R2’JT

Sample: I19654 (Female)
Location: England, Somerset, Ham Hill
Age: 400-200 BCE
mtDNA: H1c3a

Sample: I19652 (Female)
Location: England, Somerset, Ham Hill
Age: 395-205 calBCE
mtDNA: J1c2a2

Sample: I19656 (Male)
Location: England, Somerset, Ham Hill
Age: 387-198 calBCE
Y-DNA: R-DF13
mtDNA: H5’36

Sample: I16593 (Female)
Location: England, Somerset, Ham Hill
Age: 382-197 calBCE
mtDNA: H7b

Sample: I13680 (Male)
Location: England, Somerset, Ham Hill
Age: 366-176 calBCE
Y-DNA: R-L21
mtDNA: U5a2a1

Sample: I19655 (Female)
Location: England, Somerset, Ham Hill
Age: 400-100 BCE
mtDNA: H1c3a

Sample: I19855 (Male)
Location: England, Somerset, Ham Hill
Age: 400-100 BCE
Y-DNA: R-L21
mtDNA: H1ak1

Sample: I19854 (Female)
Location: England, Somerset, Ham Hill
Age: 400-100 BCE
mtDNA: J1c2a2

Sample: I11993 (Female)
Location: England, Somerset, Ham Hill
Age: 400-100 BCE
mtDNA: J1c2a2

Sample: I11994 (Female)
Location: England, Somerset, Ham Hill
Age: 400-100 BCE
mtDNA: U5a2c3a

Sample: I19657 (Female)
Location: England, Somerset, Ham Hill
Age: 356-59 calBCE
mtDNA: H5s

Sample: I21315 (Male)
Location: England, Somerset, Ham Hill
Age: 173 calBCE – 5 calCE
Y-DNA: R-M269
mtDNA: T1a1’3

Sample: I13684 (Female)
Location: England, Somerset, Meare Lake Village West
Age: 541-391 calBCE
mtDNA: W1-T119C

Sample: I11146 (Male)
Location: England, Somerset, Meare Lake Village West
Age: 400-200 BCE
Y-DNA: R-P310
mtDNA: J1c1c

Sample: I13682 (Male)
Location: England, Somerset, Mells Down, Kingsdown Camp
Age: 793-544 calBCE
Y-DNA: R-BY168376
mtDNA: H5a1

Sample: I6748 (Male)
Location: England, Somerset, Mendip, Hay Wood Cave
Age: 3956-3769 calBCE
mtDNA: H

Sample: I11145 (Male)
Location: England, Somerset, North Perrott, North Perrott Manor
Age: 166 calBCE – 14 calCE
Y-DNA: R-Z251
mtDNA: H1q

Sample: I11144 (Male)
Location: England, Somerset, North Perrott, North Perrott Manor
Age: 149 calBCE – 65 calCE
Y-DNA: R-A9857
mtDNA: H5’36

Sample: I5365 (Female)
Location: England, Somerset, Priddy
Age: 103 calBCE – 107 calCE
mtDNA: U5a1b1e

Sample: I11995 (Female)
Location: England, Somerset, South Cadbury, Cadbury Castle
Age: 742-399 calBCE
mtDNA: H2a5

Sample: I21303 (Female)
Location: England, Somerset, South Cadbury, Cadbury Castle
Age: 153 calBCE – 25 calCE
mtDNA: H2a5

Sample: I21302 (Male)
Location: England, Somerset, South Cadbury, Cadbury Castle
Age: 46 calBCE – 117 calCE
Y-DNA: R-DF13
mtDNA: K1a-T195C!

Sample: I6776 (Male)
Location: England, Somerset, Storgoursey, Wick Barrow
Age: 2400-2000 BCE
Y-DNA: R-P312
mtDNA: R

Sample: I21306 (Male)
Location: England, Somerset, Tickenham, Diamond Cottage
Age: 2200-1400 BCE
Y-DNA: R-BY31082
mtDNA: H1an1

Sample: I21305 (Male)
Location: England, Somerset, Weston-super-Mare, Grove Park Road
Age: 800 BCE – 100 CE
Y-DNA: R-DF13
mtDNA: H1

Sample: I16596 (Male)
Location: England, Somerset, Worlebury
Age: 400-50 BCE
mtDNA: H3b-G16129A!

Sample: I13681 (Male)
Location: England, Somerset, Worlebury
Age: 400-50 BCE
mtDNA: H3b-G16129A!

Sample: I11143 (Male)
Location: England, Somerset, Worlebury
Age: 352-53 calBCE
Y-DNA: R-FT5780
mtDNA: H3b-G16129A!

Sample: I13726 (Male)
Location: England, Somerset, Worlebury
Age: 351-52 calBCE
Y-DNA: R-BY23964
mtDNA: H13a1a1

Sample: I11991 (Male)
Location: England, Somerset, Worlebury
Age: 349-50 calBCE
Y-DNA: R-DF13
mtDNA: H3b-G16129A!

Sample: I11992 (Male)
Location: England, Somerset, Worlebury
Age: 343-50 calBCE
Y-DNA: R-DF13
mtDNA: H3b-G16129A!

Sample: I11142 (Male)
Location: England, Somerset, Worlebury
Age: 197-44 calBCE
Y-DNA: R-PR1289
mtDNA: H3b-G16129A!

Sample: I16619 (Male)
Location: England, Sussex, Brighton, Bevendean
Age: 361-106 calBCE
mtDNA: H49

Sample: I16617 (Female)
Location: England, Sussex, Brighton, Black Rock
Age: 777-516 calBCE
mtDNA: H4a1a1a

Sample: I16615 (Female)
Location: England, Sussex, Brighton, Coldean Lane, Varley Hall
Age: 1259-912 calBCE
mtDNA: K1c1

Sample: I14543 (Female)
Location: England, Sussex, Brighton, Ditchling Road
Age: 2450-1600 BCE
mtDNA: K1a4a1g

Sample: I16616 (Female)
Location: England, Sussex, Brighton, Mile Oak
Age: 1410-1227 calBCE
mtDNA: H13a1a1

Sample: I14552 (Male)
Location: England, Sussex, Brighton, Moulsecoomb
Age: 92 calBCE – 110 calCE
Y-DNA: R-P312
mtDNA: J1c2

Sample: I14553 (Male)
Location: England, Sussex, Brighton, Roedean Crescent
Age: 1954-1749 calBCE
Y-DNA: R-S15808
mtDNA: H5c

Sample: I14551 (Female)
Location: England, Sussex, Brighton, Slonk Hill
Age: 514-234 calBCE
mtDNA: H6a1a

Sample: I7632 (Male)
Location: England, Sussex, Brighton, Slonk Hill
Age: 391-203 calBCE
Y-DNA: R-CTS4528
mtDNA: H1

Sample: I14550 (Female)
Location: England, Sussex, Brighton, Slonk Hill
Age: 700 BCE – 900 CE
mtDNA: H3-T152C!

Sample: I16618 (Female)
Location: England, Sussex, Brighton, Surrendon Road
Age: 787-544 calBCE
mtDNA: K1a4

Sample: I14549 (Female)
Location: England, Sussex, Brighton, Woodingdean
Age: 401-208 calBCE
mtDNA: H1

Sample: I27379 (Male)
Location: England, Sussex, North Bersted
Age: 174-51 calBCE
Y-DNA: R-FGC56332
mtDNA: H7d

Sample: I27380 (Male)
Location: England, Sussex, Westbourne, ‘Racton Man’
Age: 2453-2146 cal BCE
Y-DNA: R-Z290
mtDNA: H3k1

Sample: I2611 (Male)
Location: England, Tyne and Wear, Blaydon, Summerhill
Age: 3092-2905 calBCE
Y-DNA: R-L21
mtDNA: U5a2d1

Sample: I14837 (Female)
Location: England, West Yorkshire, Dalton Parlours
Age: 381 calBCE – 6 calCE
mtDNA: K1a4a1c

Sample: I14347 (Male)
Location: England, West Yorkshire, Wattle Syke
Age: 371-176 calBCE
Y-DNA: R-DF23
mtDNA: K2a

Sample: I14348 (Female)
Location: England, West Yorkshire, Wattle Syke
Age: 368-173 calBCE
mtDNA: U3a1c

Sample: I14353 (Male)
Location: England, West Yorkshire, Wattle Syke
Age: 349-51 calBCE
Y-DNA: R-L21
mtDNA: U5b2a1a1

Sample: I14352 (Female)
Location: England, West Yorkshire, Wattle Syke
Age: 193-6 calBCE
mtDNA: K2a

Sample: I14351 (Female)
Location: England, West Yorkshire, Wattle Syke
Age: 193-6 calBCE
mtDNA: K2a

Sample: I14359 (Male)
Location: England, West Yorkshire, Wattle Syke
Age: 200 BCE – 100 CE
mtDNA: J1c1

Sample: I14360 (Female)
Location: England, West Yorkshire, Wattle Syke
Age: 151 calBCE – 62 calCE
mtDNA: J1c1

Sample: I14200 (Male)
Location: England, Wiltshire, Amesbury Down
Age: 2470-2239 calBCE
Y-DNA: R-L151
mtDNA: K1b1a

Sample: I2565 (Male)
Location: England, Wiltshire, Amesbury Down
Age: 2456-2146 calBCE
Y-DNA: R-L21
mtDNA: W1-T119C

Sample: I2419 (Female)
Location: England, Wiltshire, Amesbury Down
Age: 2393-2144 calBCE
mtDNA: H1

Sample: I2598 (Male)
Location: England, Wiltshire, Amesbury Down
Age: 2139-1950 calBCE
Y-DNA: R-P310
mtDNA: H

Sample: I19287 (Female)
Location: England, Wiltshire, Amesbury Down
Age: 761-422 calBCE
mtDNA: K1b1a

Sample: I16602 (Female)
Location: England, Wiltshire, Amesbury Down
Age: 734-403 calBCE
mtDNA: H1aq

Sample: I16600 (Male)
Location: England, Wiltshire, Amesbury Down
Age: 713-381 calBCE
Y-DNA: R-P310
mtDNA: T2b1

Sample: I16599 (Male)
Location: England, Wiltshire, Amesbury Down
Age: 411-208 calBCE
Y-DNA: R-DF13
mtDNA: T2b1

Sample: I16601 (Female)
Location: England, Wiltshire, Amesbury Down
Age: 343-43 calBCE
mtDNA: H17

Sample: I21309 (Male)
Location: England, Wiltshire, Battlesbury Bowl
Age: 354-57 calBCE
Y-DNA: R-FGC33840
mtDNA: X2b-T226C

Sample: I21307 (Male)
Location: England, Wiltshire, Battlesbury Bowl
Age: 346-52 calBCE
Y-DNA: R-P310
mtDNA: H7d

Sample: I21310 (Female)
Location: England, Wiltshire, Battlesbury Bowl
Age: 386 calBCE – 58 calCE
mtDNA: U4c1

Sample: I21311 (Female)
Location: England, Wiltshire, Battlesbury Bowl
Age: 336-49 calBCE
mtDNA: H16-T152C!

Sample: I21308 (Male)
Location: England, Wiltshire, Battlesbury Bowl
Age: 356 calBCE – 110 calCE
Y-DNA: R-P312
mtDNA: J1c1b

Sample: I21313 (Male)
Location: England, Wiltshire, Casterley Camp
Age: 354-57 calBCE
Y-DNA: R-P312
mtDNA: H3g

Sample: I21312 (Male)
Location: England, Wiltshire, Casterley Camp
Age: 343-51 calBCE
Y-DNA: R-BY129194
mtDNA: J1b1a1

Sample: I21314 (Female)
Location: England, Wiltshire, Casterley Camp
Age: 342-51 calBCE
mtDNA: V23

Sample: I16595 (Female)
Location: England, Wiltshire, Longbridge Deverill, Cow Down
Age: 387-204 calBCE
mtDNA: T2b9

Sample: I12608 (Female)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 1055-904 calBCE
mtDNA: H3ap

Sample: I12614 (Female)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 1100-800 BCE
mtDNA: K1a1b1

Sample: I12612 (Female)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 1100-800 BCE
mtDNA: U1a1a

Sample: I12611 (Female)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 1100-800 BCE
mtDNA: I2

Sample: I12613 (Female)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 1100-800 BCE
mtDNA: H1

Sample: I12624 (Female)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 900-800 BCE
mtDNA: H3

Sample: I12610 (Male)
Location: England, Wiltshire, Potterne, Blackberry Field
Age: 765-489 calBCE
Y-DNA: R-M269
mtDNA: J1c1

Sample: I19858 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 1532-1431 calBCE
Y-DNA: R-Z290
mtDNA: J2b1a

Sample: I19857 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 1518-1425 calBCE
Y-DNA: R-L617
mtDNA: J2b1a

Sample: I19859 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 1504-1403 calBCE
Y-DNA: I-S2497
mtDNA: H3

Sample: I19860 (Female)
Location: England, Wiltshire, Rowbarrow
Age: 1503-1401 calBCE
mtDNA: T2b21

Sample: I19867 (Female)
Location: England, Wiltshire, Rowbarrow
Age: 780-541 calBCE
mtDNA: H3-T16311C!

Sample: I19861 (Female)
Location: England, Wiltshire, Rowbarrow
Age: 779-541 calBCE
mtDNA: U2e2a1c

Sample: I13688 (Female)
Location: England, Wiltshire, Rowbarrow
Age: 775-516 calBCE
mtDNA: H1-C16239T

Sample: I19868 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 771-476 calBCE
Y-DNA: R-DF13
mtDNA: T2e1a

Sample: I19862 (Female)
Location: England, Wiltshire, Rowbarrow
Age: 767-423 calBCE
mtDNA: H5a1f

Sample: I13689 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 753-411 calBCE
Y-DNA: R-BY4297
mtDNA: K1a3a

Sample: I13690 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 750-408 calBCE
mtDNA: H1b3

Sample: I19863 (Male)
Location: England, Wiltshire, Rowbarrow
Age: 460-382 calBCE
Y-DNA: R-DF13
mtDNA: N1a1a1a2

Sample: I4949 (Male)
Location: England, Wiltshire, Winterbourne Monkton, North Millbarrow
Age: 3624-3376 calBCE
Y-DNA: I-M284
mtDNA: T2b

Sample: I8582 (Female)
Location: Isle of Man, Rushen, Strandhall
Age: 2195-1973 calBCE
mtDNA: H2a1e1

Sample: I12312 (Male)
Location: Scotland, Argyll and Bute, Isle of Ulva, Ulva Cave
Age: 3751-3636 calBCE
Y-DNA: I-P214
mtDNA: K1a-T195C!

Sample: I12314 (Female)
Location: Scotland, Argyll and Bute, Oban, Carding Mill Bay II
Age: 3647-3533 calBCE
mtDNA: T2b

Sample: I12313 (Female)
Location: Scotland, Argyll and Bute, Oban, Carding Mill Bay II
Age: 3700-3350 BCE
mtDNA: T2b

Sample: I12317 (Male)
Location: Scotland, Argyll and Bute, Oban, Carding Mill Bay II
Age: 3629-3377 calBCE
Y-DNA: I-A8742
mtDNA: H5

Sample: I2658 (Male)
Location: Scotland, Argyll and Bute, Oban, Macarthur Cave
Age: 4000-3700 BCE
mtDNA: W1-T119C

Sample: I3137 (Male)
Location: Scotland, Argyll and Bute, Oban, Raschoille Cave
Age: 3800-3000 BCE
Y-DNA: I-S2599
mtDNA: HV0-T195C!

Sample: I3139 (Female)
Location: Scotland, Argyll and Bute, Oban, Raschoille Cave
Age: 3800-3000 BCE
mtDNA: H45

Sample: I16498 (Female)
Location: Scotland, East Lothian, Broxmouth
Age: 750-404 calBCE
mtDNA: H2a1

Sample: I2692 (Female)
Location: Scotland, East Lothian, Broxmouth
Age: 727-396 calBCE
mtDNA: H2a1

Sample: I16422 (Male)
Location: Scotland, East Lothian, Broxmouth
Age: 364-121 calBCE
Y-DNA: R-L151
mtDNA: H3-T152C!

Sample: I2695 (Male)
Location: Scotland, East Lothian, Broxmouth
Age: 364-121 calBCE
Y-DNA: R-P312
mtDNA: H2a1

Sample: I2694 (Female)
Location: Scotland, East Lothian, Broxmouth
Age: 361-110 calBCE
mtDNA: H1ak1

Sample: I2696 (Female)
Location: Scotland, East Lothian, Broxmouth
Age: 355-55 calBCE
mtDNA: U5a2b4a

Sample: I16503 (Male)
Location: Scotland, East Lothian, Broxmouth
Age: 349-51 calBCE
Y-DNA: R-Z30597
mtDNA: H1ak1

Sample: I16416 (Male)
Location: Scotland, East Lothian, Broxmouth
Age: 346-51 calBCE
Y-DNA: R-Z30597
mtDNA: H3-T152C!

Sample: I2693 (Male)
Location: Scotland, East Lothian, Broxmouth
Age: 197 calBCE – 1 calCE
Y-DNA: R-P310
mtDNA: H3-T152C!

Sample: I16504 (Male)
Location: Scotland, East Lothian, Broxmouth
Age: 42 calBCE – 116 calCE
Y-DNA: R-DF13
mtDNA: H1as

Sample: I16448 (Female)
Location: Scotland, East Lothian, Innerwick, Thurston Mains
Age: 2337-2138 calBCE
mtDNA: K1b1a1

Sample: I5471 (Female)
Location: Scotland, East Lothian, Innerwick, Thurston Mains
Age: 2269-1985 calBCE
mtDNA: H1c3a

Sample: I2413 (Female)
Location: Scotland, East Lothian, Innerwick, Thurston Mains
Age: 2114-1900 calBCE
mtDNA: H1a1

Sample: I16499 (Male)
Location: Scotland, East Lothian, North Berwick, Law Road
Age: 337-43 calBCE
Y-DNA: R-ZP18
mtDNA: I2a

Sample: I16495 (Female)
Location: Scotland, East Lothian, North Berwick, Law Road
Age: 196 calBCE – 3 calCE
mtDNA: H6a1a8

Sample: I16418 (Male)
Location: Scotland, East Lothian, North Berwick, Law Road
Age: 97 calBCE – 107 calCE
Y-DNA: I-L1195
mtDNA: U5a1d2a

Sample: I16413 (Female)
Location: Scotland, East Lothian, North Berwick, Law Road
Age: 44 calBCE – 117 calCE
mtDNA: H6a1a8

Sample: I2569 (Male)
Location: Scotland, Eweford Cottages
Age: 2140-1901 calBCE
Y-DNA: R-P312
mtDNA: K1a3a

Sample: I3567 (Male)
Location: Scotland, Highland, Applecross
Age: 173 calBCE – 8 calCE
Y-DNA: R-FT221759
mtDNA: J1c3b

Sample: I3566 (Male)
Location: Scotland, Highland, Applecross
Age: 170 calBCE – 10 calCE
Y-DNA: R-L21
mtDNA: H13a1a

Sample: I3568 (Male)
Location: Scotland, Highland, Applecross
Age: 42 calBCE – 119 calCE
Y-DNA: R-A277
mtDNA: H7a1

Sample: I19286 (Male)
Location: Scotland, Highland, Embo
Age: 3331-3022 calBCE
Y-DNA: I-M170
mtDNA: J1c1

Sample: I2824 (Male)
Location: Scotland, Isle of Harris, Northton
Age: 41 calBCE – 121 calCE
Y-DNA: R-M269
mtDNA: H13a1a

Sample: I2656 (Male)
Location: Scotland, Longniddry, Grainfoot
Age: 1283-940 calBCE
Y-DNA: R-P312
mtDNA: H2a2a2

Sample: I2983 (Female)
Location: Scotland, Orkney, Bu
Age: 399-207 calBCE
mtDNA: U2e2a1c

Sample: I2982 (Male)
Location: Scotland, Orkney, Bu
Age: 395-207 calBCE
Y-DNA: R-Z16400
mtDNA: H7a1

Sample: I2799 (Male)
Location: Scotland, Orkney, Howe of Howe
Age: 152 calBCE – 22 calCE
Y-DNA: R-DF49
mtDNA: H1

Sample: I2629 (Male)
Location: Scotland, Orkney, Isbister
Age: 3350-2350 BCE
Y-DNA: I-L161
mtDNA: J1c1b

Sample: I2796 (Male)
Location: Scotland, Orkney, Point of Cott
Age: 3706-3536 calBCE
Y-DNA: I-FGC7113
mtDNA: H3

Sample: I5474 (Female)
Location: Scotland, Scottish Borders, Cumledge (Auchencraw Park)
Age: 151 calBCE – 77 calCE
mtDNA: K1a26

Sample: I2699 (Male)
Location: Scotland, South Uist, Hornish Point
Age: 159 calBCE – 26 calCE
mtDNA: V10

Sample: I16412 (Male)
Location: Scotland, Stirling, Coneypark Cairn (Cist 1)
Age: 2134-2056 calBCE
Y-DNA: I-CTS616
mtDNA: R

Sample: I27384 (Male)
Location: Scotland, West Lothian, House of Binns
Age: 90 calBCE – 110 calCE
Y-DNA: R-L21
mtDNA: H2a2a1g

Sample: I27385 (Male)
Location: Scotland, West Lothian, House of Binns
Age: 43 calBCE – 117 calCE
Y-DNA: R-L1066
mtDNA: T2b19

Sample: I16475 (Male)
Location: Wales, Clwyd, Dinorben
Age: 550-1 BCE
Y-DNA: R-P312
mtDNA: X2b

Sample: I16514 (Female)
Location: Wales, Clwyd, Dinorben
Age: 550-1 BCE
mtDNA: HV0

Sample: I16410 (Female)
Location: Wales, Clwyd, Dinorben
Age: 550-1 BCE
mtDNA: T2b

Sample: I16479 (Unknown sex)
Location: Wales, Conwy, Llandudno, Little Ormes Head, Ogof Rhiwledyn
Age: 1500-1100 BCE
mtDNA: H

Sample: I16491 (Male)
Location: Wales, Denbighshire, Llanferres, Orchid Cave
Age: 2876-2680 calBCE
Y-DNA: I-L1195
mtDNA: U5b2b

Sample: I6771 (Female)
Location: Wales, Glamorgan, Llantwit Major, Llanmaes
Age: 169 calBCE – 2 calCE
mtDNA: U4b1a

Sample: I16471 (Female)
Location: Wales, Glamorgan, Llantwit Major, Llanmaes
Age: 200 BCE – 50 CE
mtDNA: H2a

Sample: I16405 (Male)
Location: Wales, Glamorgan, RAF St Athan
Age: 397-205 calBCE
Y-DNA: R-DF13
mtDNA: K1a-T195C!

Sample: I5440 (Male)
Location: Wales, Glamorgan, St. Fagan’s
Age: 1500-1322 calBCE
Y-DNA: R-L151
mtDNA: K1c1

Sample: I2574 (Female)
Location: Wales, North Wales, Llandudno, Great Orme
Age: 1417-1226 calBCE
mtDNA: U5a1a2b

Sample: I16476 (Female)
Location: Wales, West Glamorgan, Gower Peninsula, Port Eynon, Culver Hole Cave
Age: 1600-1200 BCE
mtDNA: H24

Sample: I16488 (Male)
Location: Wales, West Glamorgan, Gower Peninsula, Port Eynon, Culver Hole Cave
Age: 1201-1015 calBCE
Y-DNA: R-L21
mtDNA: U5a1b1

_____________________________________________________________

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