Family Tree DNA Sale – Week 3 – The Cyber Monday Version

ftdna week3

DNA testing is the gift that gives twice.  Once to the person you give the kit or upgrade to and once to you, presuming you’re related to the recipient.

Actually, that’s not quite the whole story – it’s the gift that keeps on giving – forever.

If you’re testing Y DNA, who knows what descendants of a common ancestor hundreds of years ago will come along and want to know about the story told by that ancestor’s DNA.  And that information can only be found by testing direct paternal line descendants of that man today.  Everyone descended from that man but not on a direct paternal (surname) line needs someone who does carry that surname to test.

Same story for mitochondrial DNA – except fewer people have tested, so it makes those tests even more valuable.  They document the direct matrilineal line – your mother’s mother’s mother’s line on up the line until you run out of mothers.

And autosomal DNA – well – that’s like winning at the cousin slot machine.  When your results come back, the cousins just come pouring in.  It’s jackpot day!

Family Tree DNA’s holiday sale continues, with new weekly discounts arriving every Monday.  If you are a customer and don’t received your coupon in an e-mail, just sign on to your account and your package awaits you on your home page.

Like always, share this week’s coupon codes that you’re not using and which tests they apply to in the comments below – and look for something you need.  If you use one, please place a note on that comment.  Enjoy and click here to redeem coupons and to order kits and upgrades.

Irene Charitas (c1665-c1694) and Her Aching Mother’s Soul, 52 Ancestors #100

For a very long time, and on most online trees, Irene Charitas is listed as the wife of Johann Michael Mueller who was born about 1655 in Zollikoffen, Switzerland and who died in 1695 in Steinwenden, Germany.  Her last name is listed as Charitas, but it isn’t.  Charitas is Irene’s middle name.

At this time in history, in Germany and the Germanic speaking Protestant regions of Europe, females were given two names, a first “saints” name and a second name by which they were typically called.  Irene is quite unusual for a Saint’s name and Charitas is very unusual for a middle name.  So unusual in fact that I’ve only seen it one other time, ever.

Charitas is a Latin word meaning charity and “for the love of God.”  Charitas, or charity, is one of the 7 virtues.

Steinwenden 5

We don’t know where Irene was born, but what we do know is that we first find her as Johann Michael Mueller’s wife in Steinwenden, Germany, shown above and below, when she gives birth to the first child recorded to this couple in the church records in 1685.  Could this couple have lived there, or had children elsewhere, previously?  Of course.  Could they have had other children that were baptized in a different church?  Yes.  Michael was born in 1655, so if Irene was his age, they could have married by about 1673-1675 and had another 5 children or so before they appear in the Steinwenden records.  But did they?  We’ll never know for sure, but there is no evidence today to suggest such.

Steinwenden 6

Unfortunately, the only part of the original Steinwenden church that survives today is the bell tower.

Steinwenden 4

Sometime in the early 1680s, the Mueller family arrived in Steinwenden from the Zollikoffen area near Bern, Switzerland. It’s likely that Irene’s family was among the same immigrant group – but we just don’t know and to the best of my knowledge, no research has been done on that topic.  Furthermore, the Johann Michael Mueller family could have made an intermittent stop along the way that we are unaware of.  In other words, Michael could have married Irene Charitas anyplace between Zollikoffen and Steinwenden.

I’m not quite sure how Charitas became her last name on the internet.  Perhaps it’s an assumption based on the fact that her middle name is an unfamiliar name and someone assumed it was her last name.  In any event, it’s been that way for years now and I’m hopeful that records from the actual church can help reduce or eliminate this misinformation.  I’m currently in the process of having the church records retranslated by a professional German genealogist, just to be sure.

When our cousin, the Reverend Richard Miller visited the church in Steinwenden in 1996, the church historians and a German genealogist prepared a summary of the church records involving Johann Michael Mueller, shown below.

In all of the birth records of children born to Michael, Irene Charitas was his wife, and Charitas was not her birth name.  If the child born in 1685 was their first, then Irene Charitas was likely born about 1665, give or take a couple years in either direction.

Steinwenden 7

Recently, Richard sent me the original record of Johann Michael Mueller’s birth from the Steinwenden church.  It’s the second to last entry, below.

Miller 1792 birth steinwenden

Needless to say, I can’t read this, on two fronts, the language and the script, which is why I’m having this and the other records retranslated.

The next we hear of Irene is a church record for a confirmation of Irene Charitas Schlosser, a daughter of Conrad Schlosser, of Steinwinden.  Often, children were named after their godparents with the idea that the Godparents were relatives and they were the appointed relatives responsible for the religious education of the child – and whether spoken or unspoken, it was also expected that if the parents died, the Godparents would raise the children – or at least the one(s) named for them.  Unfortunately, in the age of marginal medical care, no antibiotics and an era where every pregnancy was high risk, that happened all too often.  So, it appears that Conrad Schlosser’s daughter was named for Michael Mueller’s wife, Irene Charitas.

It’s likely that Irene was in some way related to Conrad.  She could have been his sister or aunt or a favorite cousin.  Or, Conrad could have been related to Johann Michael Mueller.  One way or another Conrad trusted Irene enough to name his daughter after her, making Irene Charitas Mueller the first in line to raise her namesake should something happen to Conrad and his wife.  Additional research on the Schlosser family church records is in order.

Steinwenden 1

The first record on this transcriptions says that Jacob Ringeisen of Schweitz was “serving for his cousin” Michael Muller.

In other words, even though the daughter was named for Irene, Michael’s wife, Irene wasn’t present, possibly due to pregnancy herself, and apparently neither was Michael.  However, Michael’s cousin in essence represented Michael and the couple’s commitment at the baptism.

Of course, now this makes me ask just how Michael and Jacob were cousins, and was it through marriage via Irene Charitas?  It looks like we may have yet another family connection hint.  So often in these old church records there is so much more buried in the details that is missed if all you get is a translation of the actual “event.”

In genealogy, always, always, more questions.

Irene Charitas’ life was short.  She probably died before she was 30.  There are no more known records of her, at least not directly.

What we do know is that the last child in these church records is born to Irene and Michael in 1692.  This is the only one of the six children she bore that lived.  This is an incredibly sad story that seems to stretch beyond just “bad luck.”   

Child Birth Death Age at Death
Johann Nicholas Muller June 5, 1685 June 6, 1685 1 day
Johann Abraham Muller July 9, 1688 1696 Less than 6 months
Samuel Muller April 30, 1687 April 30, 1687 Shortly after birth
Catherine Barbara Muller June 7, 1688 June 21, 1691 3 years, 2 weeks
Eva Catherine Muller April 24, 1691 June 29, 1691 2 months
Johann Michael Muller October 5, 1692 1771 78 years

Looking at these children’s deaths, I find the month of June, 1691 particularly heartbreaking.  Clearly, something contagious was occurring and both of Irene’s children died, 8 days apart.  I wonder if the church records reflect a rash of deaths within the village.  Just 11 months later, she would bear her 6th child.  I bet those months between June of 1691 and May of 1692 were living Hell for Irene, between the sorrow and grief of losing her children and the uncertainly of the one she was carrying.

Fortunately for me, Johann Michael Mueller, the second, born in 1692, named after his father, did live, as he is my ancestor.

Johann Michael Mueller Sr. died in Steinwenden in 1695, just three years later. For some reason, from 1692 to 1695, there were no more children born to Johann Michael Mueller and Irene Charitas – nor to Johann Michael Mueller and anyone else.

Why is this important?  Because another rumor that has been rampant over the years is that Johann Michael Mueller was married to Anna Loysa Regina and that she was the mother of Johann Michael Mueller.  At least I was able to figure out where this information originated.

On September 29, 1695, Anna Loysa Regina married Jacob Stutzman in Steinwenden, although I have not seen the original record myself.  She is noted at that time as being the widow of Michael Mueller.

For Anna Loysa Regina to be the window of Michael Mueller, that means that Irene Charitas died sometime after giving birth to Michael Jr. on October 5, 1692 and sometime before Michael Sr.’s death on January 31, 1695, just 2 years and 3 months later, allowing Michael Sr. enough time to remarry after Irene’s death and before his own.  Remarriage often didn’t take much time actually, given that most people already knew each other through church and it was simply a matter of taking stock of the available spouses and making a choice from the willing and most compatible selection.  No, it was not about love but one would hope it was at least about like and that love evolved.  Regardless, marriage was a practical matter of survival as men and women needed each other’s assistance in the daily activities of living and raising children.  Michael would have had a small child who needed a mother.

Michael and Anna Loysa Regina must not have been married long, because there are no children recorded to she and Michael, but she did go on to have children with Jacob Stutzman, one as late as 1706.  Jacob Stutzman Jr. born in 1706, and Johann Michael Mueller Jr., born in 1692, step-brothers of a sort, would in 1727 immigrate to America together.

If all of these records have been accurately translated, Irene Charitas probably died about 1694, possibly in childbirth.  In the natural order of things, March or April 1694 would be about 18 months after Michael was born, representing the typical spacing between children.  Why no record of her death exists in the church records is a mystery.  Perhaps we need to look again, and maybe in the surrounding church records as well.

Cemetery plots in Germany, as is customary in Europe, are reused.  In some cases, they continue within the family, with generation upon generation (pardon the pun) being buried in the same location.  In other cases, the grave is considered “abandoned” if no one pays upkeep, and the site is reused at the discretion of the church.  Gravesites that aren’t abandoned are still reused, but generally by the family and perhaps not as quickly as abandoned graves.  While this is very foreign to those of us in the US, if Europeans did not employ some “recycling” burial strategy, the entire continent would be blanketed with cemeteries and there would no room for the living.

Being someone who wonders about everything, I asked at a Dutch church during a European visit in 2014 about what happened if there were still bones in the grave when they set about burying the next person.  I’m glad I asked, because I then discovered that those little buildings in or near cemeteries weren’t what I thought.  I assumed they were the gardener’s or sextant’s shed, containing things like shovels, lawnmowers, etc.  Well, I was wrong.  Those little buildings are ossuaries containing the bones of the former inhabitants of graves.  The photo below is the ossuary in Wolsum, the Netherlands.

Ossuary Wolsum

This is truly the final resting place until the bones turn to dust, generally stacked something like cordwood with similar types of bones stacked with like bones on shelves.  Yes, seriously.  Once moved from the grave to the ossuary, the bones are not kept together as a “person.”  This photo is an ossuary in Hallstatt, Austria.

Ossuary Austria

From a DNA perspective, these ossuaries, found in almost all cemeteries, are just torture to me, because I can just see the DNA of my ancestral lines in that ossuary, all mixed in with the DNA of the other families…which are probably mine as well, given that these people married their neighbors in the community for generations.  There they are, my ancestors and their DNA, right in front of me, but entirely anonymous and completely unidentifiable.  If we knew who they were, we could obtain the Y and mtDNA lineage of every family in the village, including mine!

The bones in the ossuaries are just waiting to finish turning to dust – a process that takes longer than they are allowed to rest in the ground.  So a grave in Europe is not a place of perpetual rest, it’s a temporary resting point but not the last stop on the journey.  I just can’t help but think what a wonderful scientific study it would be to analyze the bones in an ossuary and compare the results to the DNA of the current village inhabitants, and those descendants who moved away.  And yes, you know I’d be in the front of the line, volunteering.  You could reconstruct an entire village in the 1700s or maybe 1800s from their DNA – maybe even further back.  You could tell who settled there, where they were from originally… you could learn so much.  But back to reality….

Not only do we not know where Irena Charitas and her infant children were buried, their dust assuredly shares that location today with several subsequent generations of Germans, most likely not her descendants because her only known descendant immigrated to America in 1727 with his Stutzman step-brother.  Irene Charitas’ son Johann Michael Mueller, Jr. never knew his mother or father, never remembered seeing his mother’s face, beaming down at him, so joyous that he was alive.  He had no memory of her loving touch.  He was raised by his step-mother and her subsequent husband, Jacob Stutzman, after both of Michael’s parents died by the time he was three.

There was no happy ending for Irene Charitas.  In fact – it seems that her entire adult lifetime was filled with serial grief, except for those few brief months when she and baby Michael both lived.  Irene Charitas’ grief was caused by the births and deaths of 5 children in 5 years, followed by her own death not long after her 6th child was born and survived.  Then, the terrible irony.  When a child finally lives, she herself succumbs.

I can only imagine the excitement Irene felt about her first pregnancy, followed by the shattering death of the baby.  Surely, she would have told herself that it wouldn’t happen again.  It was a first birth, probably difficult.  The second one would be easier.  Just a few months later, she became pregnant again and full of hope, only to have her dreams shattered again with the death of that child.  And then again…and again and again, year after year after year.  Just five years after that first baby died, she was pregnant for her sixth child.  I wonder if she started out in dread when she discovered she was pregnant again, never allowing herself to be excited, to plan, to hope for that baby to live.  I could understand how she might feel that way after 5 dead babies in 5 years.  I know how frightened I was when I was pregnant for my third child after my second child died.

And then the baby lived but she died.  Oh, the horrible irony.  Poor Irene.  In death, leaving behind her one child that lived.  She must have fought the grim reaper with every ounce of her being until the very end.  But it wasn’t enough.  It just wasn’t enough.

I hope that Irene Charitas was able to see, from afar, her son, Johann Michael Mueller Jr. growing up strong, being raised by his step-mother and step-father in a pious pietist home and that it helped sooth her aching mother’s soul.

Facebook – A Sense of Connection…and Betrayal

First of all, let me say I’m a fan of Facebook.  Yes, it has warts and there are aspects I dislike, but overall, I feel it gives us a sense of connection that is otherwise impossible to have in our geographically dispersed world.  In our hectic world today, most of us no longer live down the block or next door to our parents, siblings, aunts and uncles.  We may not even know our cousins, aside from their names.  Families are no longer nuclear or close by.

When I was younger, the Christmas letter was what connected people across the years – and each one was hand written in the Christmas card.  Later, when computers and word processing debuted, one letter was written, several copies printed and a copy put into each card.  People got offended due to the impersonal nature of the letter.  Times were changing.

Today, the Christmas card letter is nearly obsolete, in any format.  I haven’t sent cards in years.  (I know, bad cousin, bad cousin.)  I actually received an annual card and letter from someone I had no idea who was, for years.  And by that time I was too embarrassed to ask who they were and how we were connected.

So I was extremely glad when Facebook came along, because it allows us to have a sense of connection and continuity with our family, and friends….along with people who want to “friend” us who are unknown to us.  Yes, unfortunately, the world of “stranger danger” has come along with the connectivity for both children (who aren’t supposed to be on Facebook) and adults.

Facebook is also a wonderful way to get to know your new cousins, often found through genealogy and genetic genealogy.  It’s a lot more personal than an occasional e-mail or letter – and you get a much more balanced perspective of their life – their interests and who they are.

However, the way Facebook inherently works, or doesn’t, can sometimes lead to hurt feelings.  When you’re friends with someone on Facebook, you expect that they will see all of your posts and you will see all of theirs.  Right???  Wrong.  And that’s exactly what leads to hurt feelings.

Twice now within the past month, someone who I’m Facebook friends with has posted something expressing dismay and hurt feelings about what didn’t happen.  They had posted something serious, asking for prayers, and they did not hear from many of the people they expected to hear from.  That’s hurtful – especially if you think it’s intentional.  But, don’t get your knickers in a knot just yet – because it’s probably NOT an act of neglect or worse yet, a slap-in-the-face type betrayal from your family and friends. It’s all about how Facebook works, or doesn’t.

Facebook is not like mailing a letter or sending an e-mail directly to someone.  You can have some level of expectation that they received the letter or e-mail, although not 100% – but that’s not the case with Facebook.  Facebook SELECTS posts to display on friends newsfeeds.

How does Facebook do that and which posts?  Who knows – but they do.  In both cases mentioned above, the people later expressed how hurt they were that no one or few replied.  I went back and looked in my feed, and neither had been posted to my timeline.

I looked at their postings on their page, and sure enough, there they were.  I felt awful, and so did other people who also replied that they had never seen the prayer requests.  Just think how many people would have never mentioned how hurt they were and just suffered in silence.  The rest of us would have never known and they would have thought they were slighted.  This may sound trivial, but it’s certainly not when the topic at hand is something this serious.  If they had understood how Facebook works, and doesn’t, it would have helped a great deal and avoided those unnecessary hurt feelings.

How Facebook Works – and Doesn’t

So, how does Facebook posting work?  I’ve received permission to use my friend, Janine, as an example.  Janine and I have several common interests, genealogy, genetic genealogy, NASCAR and photography.  Suffice it to say, most of her posts are of interest to me.

Let’s take a look at some options you can check to see if you’re receiving the maximum about of information from another person – or maybe to tone it down if you’re receiving too much.

This assumes that you are Facebook friends with someone.  All of the setting we’re going to review are accessed from their page.

To see their page, click on their name or enter their name in the search box and then click on their name.

facebook profile

Following People

facebook friends

By clicking on the “Folllowing” box, “See First” gives you the option to always see this person’s posts at the top of your feed if you would like.  I don’t think that this means ALL of their posts – but when Facebook decides to put their post on your news feed, it will be at the top.

Unfollow

The small “unfollow” option at the bottom can be a lifesaver – but in a bit of a different way.

If you find that someone is posting things you don’t really want to see – you can click on “Unfollow Lisa.”  This does NOT unfriend her – so you’ll still be friends – and she won’t know that you unfollowed her.  Unfollow just means her posts won’t be included on your newsfeed.  And you can undo it quickly by the click of a button.

I refer to this as the “political and religious filter.”  Unfortunately, I wish there really was a way to filter out only content of a specific type, but there isn’t, so if you unfollow someone, their posts will never be placed in your timeline.

Why do people unfollow others?  Mostly, because they have a difference of opinion on something and the person on the receiving end doesn’t want to see those specific types of posts.  These types of postings seems to increase exponentially during the political season or after something happens that is contentious and elicits strong feelings in either or both directions.  Like what?  Well, judging by today’s postings on my newsfeed, those topics would include refugees, abortion, gun control and of course the ever popular political candidates.   No, nothing controversial there.

Yes, I have opinions on all of these.  No, you will never know what they are unless you are a very close friend or a very close family member.  You will never find my opinions on volatile or controversial subjects on Facebook.  First, I don’t want to start a war.  Second, I fully realize that no one really wants to see any more of this stuff and third, none of it is going to change anyone’s mind about anything.

If you have doubts, just think Thanksgiving table when Uncle Joe and Uncle Ted had the “discussion” about Protestant vs Catholicism, or racism, or whatever that difficult topic was at your house.  That was the quietest Thanksgiving ever…until my mother jumped up and said, “Amen” to close the topic and asked who wanted pie.  The rest of us were greatly relieved and Mom had a little “talking to” with both of them later, forbidding them from ever doing that again at Thanksgiving.  Facebook is no different except Mom isn’t around to solve things anymore and to send the offenders to the adult version of timeout, which, on Thanksgiving afternoon was in front of the TV glued to football.

Worse yet, much of what is posted online as fact isn’t.  http://www.snopes.com is a good resource to figure out what is fact and what is fiction.

One of the best ways to get yourself quickly unfollowed by me is to participate in what I consider to be manipulative behavior.

facebook manipulation

Telling me that if I don’t do something it means that I “don’t love Jesus,” God and country, my children, or something similar is the best way EVER to assure that I’ll never do what you want.  This is pure and simple manipulation and I don’t play that game – no matter what anyone thinks it means.

Asking is fine – that’s “please share.”  Telling or subtly threatening is not.  Threatening includes inferring a negative interpretation of what my noncompliant behavior means to you (see above) – and by inference anyone else reading my timeline and seeing that I did not do what you wanted.  Sorry, no one gets to paint my world except me.  Most people intensely dislike other people trying to manipulate their behavior, whether they say anything out loud or not.  Worse yet, if you do pass it on by “sharing,” then you’re the one twisting the arms of your Facebook friends.

Often these things get passed on by well-meaning but naïve people.  Sometimes the graphics harbor malware like a highly contagious flu bug – which is why the original person launched it in the first place – intending to take advantage of people who will, by virtue of guilt or arm twisting, participate in this new rendition of the old-fashioned chain letter that tells you if you don’t send a dollar to the last person on the list within 48 hours that someone in your family will die within 10 days.  Oh yes, and pass the letter on to 10 more people because in just another 10 mailings, if no one breaks the chain, you’ll receive $10,000,000,000 in dollar bills.  So not only can you prevent your family members death, you’ll get rich in the process!  Yeah, right.  How did that work out for you?

So, if you have a Facebook friend who is over-posting, posting things you don’t want to see or attempting to twist your arm, you can unfollow them without causing a ruckus and without unfriending them.  However, if you unfollow them, you will NEVER see a post from them – which may or may not be what you wanted to achieve.  You can always check their page from time to time.

If you don’t want to be unfollowed, you might considered not posting about topics that are volatile, emotional, controversial or manipulative in nature.

Friends

facebook friends2

Next, check the “Friends” status.  You can see that I have Janine marked as “get notifications” and “close friend.”

Get notifications means that I will receive notifications of comments on her postings.  If I turn “get notifications” off, I’ll only see replies to her posts that I’ve commented on or things I’ve posted to her feed.  Since she and I have lots of interests in common, I have “get notifications” selected.

Facebook says that “Close friends get priority over people who you don’t designate as close friends.”  When you share something on your timeline, one of your choices is to share with “close friends.”  I tried to verify this but did not see this designation.  What Facebook doesn’t say is whether or not close friends receive all of our postings, although based on personal experience, it appears that there is nothing you can do to receive all postings from someone.  Facebook says you “will see more of them in your newsfeeds.”

Conversely, by selecting “acquaintances,” according to Facebook, “you’ll see less of their feeds.”  I don’t know if that means less than if you select nothing or less than “close friends.”

“Family member” used to be a category, but is no longer.  My daughter-in-law who posts pictures of my grandchildren used to be designated as a family member.  Today, she is listed as a close friend and with “get notifications,” but I still don’t see all of her postings.  I check her page periodically to see which of my grandchildren’s photos I’ve missed.  Bah humbug Facebook.

The default is that nothing is checked.

Unfriend

The “Friend” box also holds the dreaded “Unfriend” button, at the bottom.  Unfriend means that person can no longer see what you post and vice versa.  You, and they, can see in a number of ways if you and they are still friends or not.

So, if you unfriend someone, they will, or can, know about it.  They are not notified but if they check, it’s obvious.  I think I’ve only ever unfriended two people who were clearly toxic.  But then, I’m extremely restrictive about who I become Facebook friends with because spammers and scammers abound.

The only way to undo an unfriend is by sending that person a friend request again.

Blocking People

This brings us to the topic of blocking people.

facebook dots

If you click on the three little dots, there are more options, including block.

Block means just that – entirely.  They can’t send you a friend request and you won’t ever see their posts anyplace they post.  They won’t see what you post either – in essence, neither of you exists in the others world.  So if you and they are in a common group or have a common friend or family member – you won’t see their posts or comments and they won’t see yours.  Sometimes, this means that what you do see doesn’t make sense because posts made by them or replies from them are missing and are referenced by other people, leaving you scratching your head.

Other Options

Just out of curiosity, I tried “Poke.”  It just sent Janine a note saying I poked her and asks if she wants to poke me back.  Yes, some programmer had far too much time on their hands one day it seems.

facebook friendship view

The “See Friendship” tab shows things that we’ve done together.  It’s kind of like a diary.  You can see here that Janine posted something on my timeline about fabric.  This can actually be very useful if I can manage to remember that it’s Janine who sent me something in particular that I’m hunting for.  You know, like the recipe I want to use this week for Thanksgiving and can’t find.

Oddities

facebook timeline

One last thing.  On Facebook, things change and aren’t always where you think they might be.  One day, I looked at my page and realized that posts people have posted to my timeline weren’t in the regular feed, but were on the left below all kinds of other things.  I’ve never seen this since, but just suffice it to say, keep your eyes open and look around.  The only thing that is constant is change – in life and on Facebook.

Private Messages

Check for private messages periodically.  The message icon is at the top of your page, next to the people icon which is where you see who has requested to be your friend.  If people message you, they presume you received, and read, the message and they are going to be hurt or offended if you don’t reply.

facebook private messages

There used to be a separate inbox that was relatively hidden where messages from people who weren’t Facebook friends ended up, but I believe that second inbox has been removed and all of your messages from friends and those not your Facebook friends are combined into one message box now.

In Summary

I hope this little spin through Facebook has helped a bit.  I hate to see something that can be such a positive tool to build communities and relationships cause hurt feelings because people don’t understand how to utilize their options to maximize their chances of getting what they want out of Facebook.  Just remember, no matter what you select, it’s Facebook and they get to choose.

Why?  Facebook is free, although I should put “free” in quotes because you’re doling out a lot of informatoin about yourself that is useful and valuable to marketers.  If you doubt this for a moment, just google some product and then watch ads for that product show up in your Facebook feed for weeks afterwards.  I wish they had an “I already bought it” button.  I saw children’s Mavis halloween costumes for weeks!

Facebook has never given us any indication they won’t change how things work, and they do change things often, and without notice.  So, stay vigilant, stay flexible, and don’t assume that your cousin is blantantly and willfully ignoring you – because there is a good chance they aren’t.  It’s just Facebook.

Living the Life You Only Hoped For

Thanksgiving is hard for some folks.  Life didn’t turn out exactly as they hoped or planned.

It’s easy for me to sometimes get tied up in the melancholy.  Thanksgiving when I was younger was a festive time on the farm.  The kitchen was overflowing onto tables in the living room. Aunts, uncles, siblings, lots of kids, sometimes foster children, boyfriends, girlfriends…the house was full. Mom and I were cooking and everyone brought a dish to pass.  It never occurred to me that one day those times would only be a memory.

It’s not like that now.  All of those people are gone, including my siblings.  In fact there are only a handful of people alive now who experienced those days and most of them are scattered to the winds.

So, I have to actively think of things to be thankful for at Thanksgiving.  Obviously, I’m thankful for my family, my children, their spouses, grandchildren and grandpuppies who do live close by.  And I’m really thankful that my husband likes to cook – and so are my kids!!!

Then, last night, on Facebook, I saw this inspirational saying by http://www.ibelieve.com.

thankful

That is just spot on.  I have never thought about things quite like this before.

And of course, my thoughts immediately turned to genetic genealogy.

Twenty years ago, DNA testing didn’t exist nor did we have any clue that it might.

Fifteen years ago, Bennett Greenspan and Max Blankfeld were just starting Family Tree DNA.  They are today the only one of the early testing companies still in business and the only one to offer a full complement of DNA tests for genealogy.  Am I ever thankful for them and their success.

Ten years ago, we thought we had come a long way because we could test males Y chromosomes to 25 or 37 markers and the female line mitochondrial DNA.  I don’t recall whether we were doing full sequence testing yet a decade ago.

Five years ago, autosomal DNA testing had just been introduced and we were ecstatic.  Little did we know it would open the floodgates.

And today, the genetic genealogy world is one I couldn’t even have dreamed of.  I wonder what the next 5 years holds.

Indeed, times have changed dramatically, and for all we’ve lost through the natural processes of life, we’ve gained incredibly.  Not only have we gained new relatives and immediate family through birth and marriage and birth…but we’ve gained the tools to get to know our distant relatives.

By distant, I mean both in terms of miles and ancient.  The new relatives who live distantly we now get to know through social media like Facebook.  One of the ways we find those new relatives is through genealogy and sometimes, DNA testing.  I’ve become very close to some of the people I’ve met through genealogy.

But I also mean distant as in distant or ancient ancestors, my great-great-great-great-great grandfather Estes.  My most distant Estes ancestor was Nicholas Ewstas born in 1495 in Deal, Kent, England.  Today, through the magic of DNA testing, I know what his entire Y chromosome looked like, through his descendants.  I know that many of us today probably share small portions of his autosomal DNA.  I know how to identify his descendants by matching them to his Y chromosome results.  I know where in the world he came from, before Kent.  I know how his ancestors got from Africa to Europe and then to England, at least roughly.

Furthermore, the more people who test, the more direct Y and mtDNA relatives I can find to complete my DNA pedigree chart.  The more I can learn about these distant ancestors, by meeting more of my distant relatives in this lifetime.  The more people who test, the more ancestors available for all of us to find!!!

My biggest regret is that I didn’t know about DNA testing back in the day – that I can’t go back and swab those aunts and uncles.  I wouldn’t make that mistake today.  I now carry swab kits in my purse.  And yes, those of you who know me know I’m dead serious.  I would test all of them for autosomal DNA, Y and mtDNA if those lines had not already been tested and posted publicly for other descendants to find.

Indeed, I am extremely fortunate to find myself living in a time of miracles I didn’t even know enough to hope for.  I am very thankful.

thankful 2

Genographic Project Publications 2015

Nat Geo logoAt the 2015 Family Tree DNA Conference, Dr. Miguel Vilar was kind enough to offer to send me the list of papers published to date as a result of the Genographic Project.  He mentioned that there are 5 additional papers in the final stages of publication, so the total for the end of 2015 will be 60 papers.  Quite an accomplishment!

Below are the titles and references plus short descriptions of the major findings.  Thank you Dr. Vilar and National Geographic.

2007

  1. Behar, D. M., Rosset, S., Blue-Smith, J., Balanovsky, O., Tzur, S., Comas, D., Mitchell, R. J., Quintana-Murci, L., Tyler-Smith, C., Wells, R. S., and The Genographic Consortium. 2007. The Genographic Project public participation mitochondrial DNA database. PLoS Genetics 3: 1083-1095.
  • This paper establishes Genographic’s database as the new standard mtDNA data repository and reports a new “Nearest Neighbor” statistical method for improved haplogroup classification, presenting learned experience from the public part of the project. It also makes publicly available a portion of the Genographic database, a process that will continue throughout project duration. This technical paper has been crucial in establishing the project’s importance in the scientific community.

2008

  1. Gan, R. J., Pan, S. L., Mustavich, L. F., Qin, Z. D., Cai, X. Y., Qian, J., Liu, C. W., Peng, J. H., Li, S. L., Xu, J. S., Jin, L., Li, H., and The Genographic Consortium. 2008. Pinghua population as an exception of Han Chinese’s coherent genetic structure. Journal of Human Genetics 53: 303-313.
  • The Han Chinese are the largest ethnic group in the world with more than 1.3 billion people, comprising 19 percent of the world population. Chinese is the language spoken by this ethnic group, which can be classified into 10 major dialects. This paper focuses on studying the genetic structure of the people speaking one of these dialects, the Pinghua people. When the genetic structure of Pinghua people was compared to the rest of the Han Chinese populations, it was observed that Pinghua populations did not directly descend from Han Chinese, who originated in the north, but from other southern populations. Thus, from a genetic point of view, the Pinghua populations represent an exception to the rest of Han Chinese populations. These results can be explained if ancestral populations of Pinghua people were not replaced by Han Chinese population, but if they assimilated the Han Chinese language and culture.
  1. Zalloua, P. A., Xue, Y., Khalife, J., Makhoul, N., Debiane, L., Platt, D. E., Royyuru, A. K., Herrera, R. J., Soria Hernanz, D. F., Blue-Smith, J., Wells, R. S., Comas, D., Bertranpetit, J., Tyler-Smith, C., and The Genographic Consortium. 2008. Y-chromosomal diversity in Lebanon is structured by recent historical events. American Journal of Human Genetics 82: 873-882.
  • Lebanon is a small country in the Middle East inhabited by almost 4 million people from a wide variety of ethnicities and religions. The results of this paper indicate that male genetic variation within Lebanon is strongly structured by religion. This unusual situation can be accounted for by two major known historical migrations into Lebanon. The Islamic expansion from the Arabian Peninsula beginning in the 7th century introduced genetic lineages typical of the Arabian peninsula into Lebanese Muslims, while the crusader activity in the 11th-13th centuries introduced Western European lineages into Lebanese Christians.
  1. Behar, D. M., Villems, R., Soodyall, H., Blue-Smith, J., Pereira, L., Metspalu, E., Scozzari, R., Makkan, H., Tzur, S., Comas, D., Bertranpetit, J., Quintana-Murci, L., Tyler-Smith, C., Wells, R. S., Rosset, S., and The Genographic Consortium. 2008. The dawn of human matrilineal diversity. American Journal of Human Genetics 82: 1130-1140.
  • African genetic diversity is unlike that found anywhere else in the world. This paper seeks to make sense of some of the most fundamental questions surrounding our earliest ancestors on the continent. Where specifically did we originate in Africa? Was it from a single group or the result of many? When do we first see African lineages appear outside of Africa? About 350 novel mitochondrial whole-genome sequences were included — doubling the existing published dataset — and the paper presented a new tree of African mtDNA diversity, reporting many novel African lineages for the first time. This paper provides an age estimate for the earliest split of humans in East Africa as one group headed south and was subsequently isolated. It explains that all humans came from a single population that split into two groups, shows that more than 99 percent of all living humans descend from one of these two groups, and suggests historical reasons for why genetic mixture did not exist between these ancient populations. It also presents evidence for the emergence of these early lineages into the Middle East and the origins of the two major non-African groups, M and N, respectively. The paper received considerable media attention — approximately 275 articles — including substantial pieces in the Economist and on CNN/BBC online.
  1. Behar, D. M., Blue-Smith, J., Soria-Hernanz, D. F., Tzur, S., Hadid, Y., Bormans, C., Moen, A., Tyler-Smith, C., Quintana-Murci, L., Wells, R. S., and The Genographic Consortium. 2008. A novel 154-bp deletion in the human mitochondrial DNA control region in healthy individuals. Human Mutation 29: 1387-1391.
  • This paper describes a novel deletion of 154 base pairs within the control region of the human mitochondrial genome that was originally identified in an anonymous Japanese public participant. It was demonstrated that this deletion is a heritable character since it was transmitted from the participant’s mother to her two sons. This is the first time that such a large deletion located in this specific portion of the control region has been observed to not have negative effects in the health of the carriers. The identification of this large heritable deletion in healthy individuals challenges the current view of the control region as playing a crucial role in the replication and regulation of the mitochondrial genome. It is anticipated that this finding will lead to further research on the reported samples in an attempt to increase our understanding of the role of specific sequences within the control region for mtDNA replication. Finally, this paper illustrates the importance of creating a large database of human genetic variation in order to discover rare genetic variants that otherwise would remain unidentified. The discovery of such rare mtDNA haplotypes will be important to identifying the relative power of adaptive and non-adaptive forces acting on the evolution of the mtDNA genome.
  1. Parida, L., Melé, M., Calafell, F., Bertranpetit, J., and The Genographic Consortium. 2008. Estimating the ancestral recombinations graph (ARG) as compatible networks of SNP patterns. Journal of Computational Biology 15: 1133-1153.
  • Traditionally the nonrecombinant, maternally inherited (mtDNA) and paternally inherited (Y chromosome) genomes have been widely used for phylogenetic and evolutionary studies in humans. However, these two genomes only represent 1 percent of the total genetic variation within an individual, and sampling just these two loci is inadequate to reconstruct with any precision the time-depth and pattern of human evolution. The scope of this paper is to elaborate on a mathematical algorithm that includes recombination patterns among human populations. This approach will allow us to use the rest of the recombining genome to reconstruct more accurately the patterns of human migration.
  1. Rossett, S., Wells, R. S., Soria-Hernanz, D. F., Tyler-Smith, C., Royyuru, A. K., Behar, D. M., and The Genographic Consortium. 2008. Maximum-likelihood estimation of site-specific mutation rates in human mitochondrial DNA from partial phylogenetic classification. Genetics 180: 1511-1524.
  • This paper presents novel algorithms to estimate how frequently each base pair of the hypervariable region of the mtDNA changes. Implementations of these algorithms will help to better investigate functionality in the mtDNA and improve current classification of mtDNA haplogroups.
  1. Zalloua, P. A., Platt, D. E., El Sibai, M., Khalife, J., Makhoul, N., Haber, M., Xue, Y., Izaabel, H., Bosch, E., Adams, S. M., Arroyo, E., López-Parra, A. M., Aler, M., Picornell, A., Ramon, M., Jobling, M. A., Comas, D., Bertranpetit, J., Wells, R. S., Tyler-Smith, C., and The Genographic Consortium. 2008. Identifying genetic traces of historical expansions: Phoenician footprints in the Mediterranean. American Journal of Human Genetics 83: 633-642.
  • The Phoenicians gave the world the alphabet and a love of the color purple, and this study shows that they left some of their genes as well. The paper shows that as many as one in 17 men in the Mediterranean basin may have a Phoenician as a direct male-line ancestor, using a novel analytical method for detecting the subtle genetic impact of historical population migrations. Its first application has been to reveal the genetic legacy of the Phoenicians, an intriguing and mysterious first-millennium B.C. trading empire. From their base in present-day Lebanon, the Phoenicians expanded by sea throughout the Mediterranean, founding colonies as far as Spain and North Africa, where their most powerful city, Carthage, was located. The world’s first “global capitalists,” the Phoenicians controlled trade throughout the Mediterranean basin for nearly a thousand years until their conquest by Rome in the 2nd century B.C. Over the ensuing centuries, much of what was known about this enigmatic people was lost or destroyed. This paper received substantial international and domestic press coverage, including an article in The New York Times.

2009

  1. Parida, L., Javed, A., Melé, M., Calafell, F., Bertranpetit, J., and The Genographic Consortium. 2009. Minimizing recombinations in consensus networks for phylogeographic studies. BMC Bioinformatics 10: Article S72.
  • This paper implements a new mathematical model to identify recombination spots in human populations to infer ancient recombination and population-specific recombination on a portion of the X chromosome. The results support the widely accepted out-of-Africa model of human dispersal, and the recombination patterns were capable of detecting both continental and population differences. This is the first characterization of human populations based on recombination patterns.
  1. El-Sibai, M., Platt, D. E., Haber, M., Xue, Y., Youhanna, S. C., Wells, R. S., Izaabel, H., Sanyoura, M. F., Harmanani, H., Ashrafian Bonab, M., Behbehani, J., Hashwa, F., Tyler-Smith, C., Zalloua, P. A., and The Genographic Consortium. 2009. Geographical structure of the Y-chromosomal genetic landscape of the Levant: A coastal-inland contrast. Annals of Human Genetics 73: 568-581.
  • This paper examines the male-specific phylogeography of the Levant and its surroundings. The Levant lies in the eastern Mediterranean region, south of the mountains of south Turkey and north of the Sinai Peninsula. It was found that the Levantine populations cluster together when considered against a broad Middle-East and North African background. However, within Lebanon there is a coastal-inland (east-west) pattern in the diversity and frequency of several Y haplogroups. This pattern is likely to have arisen from differential migrations, with different lineages introduced from the east and west.

2010

  1. Haak, W., Balanovsky, O., Sanchez, J. J., Koshel, S., Zaporozhchenko, V., Adler, C. J., Der Sarkissian, C. S. I., Brandt, G., Schwarz, C., Nicklisch, N., Dresely, V., Fritsch, B., Balanovska, E., Villems, R., Meller, H., Alt, K. W., Cooper, A., and The Genographic Consortium. 2010. Ancient DNA from European Early Neolithic farmers reveals their Near Eastern affinities. PLoS Biology 8: Article e1000536.
  • The nature and speed of the Neolithic transition in Europe is a matter of continuing debate. In this paper, new genetic analyses based on ancient human remains from the earliest farming culture in Central Europe known as the Linear Pottery Culture (5,500-4,900 years ago) indicate a shared genetic maternal affinity with modern-day Near East and Anatolia, and therefore they likely came from the Middle East. However, these lineages from the earliest agriculturalists were also distinct from the current genetic lineages observed in European populations, indicating that major demographic events continued in Europe during the Neolithic. These results point out the importance of using ancient DNA to better understand past demographic events.
  1. Melé, M., Javed, A., Pybus, M., Calafell, F., Parida, L., Bertranpetit, J., and The Genographic Consortium. 2010. A new method to reconstruct recombination events at a genomic scale. PLoS Computational Biology 6: Article e1001010.
  • A chromosomal recombination event creates a junction between two parental sequences. These recombinant sequences are transmitted to subsequent generations, and recombination is one of the main forces molding human genetic diversity. However, the information about genetic relationships among populations given by these events is usually overlooked due to the analytical difficulty of identifying the history of recombination events. This paper validates and calibrates the IRiS software for inferring the history of recombination events, allowing the creation of novel recombinational “markers” known as recotypes, which can be analyzed in a similar way to standard mutational markers.
  1. Qin, Z., Yang, Y., Kang, L., Yan, S., Cho, K., Cai, X., Lu, Y., Zheng, H., Zhu, D., Fei, D., Li, S., Jin, L., Li, H., and The Genographic Consortium. 2010. A mitochondrial revelation of early human migrations to the Tibetan Plateau before and after the Last Glacial Maximum. American Journal of Physical Anthropology 143: 555-569.
  • The Tibetan Plateau was long considered one of the last areas to be populated by modern humans. Recent archaeological, linguistic and genetic findings have challenged this view. In this paper, maternal lineages of 562 individuals from nine different regions within Tibet have been analyzed to further investigate the timing and routes of entry of humans into the plateau. The maternal diversity in Tibet primarily reflects northern East Asian ancestry, likely reflecting a population expansion from this region into the plateau prior to the Last Glacial Maximum (LGM) ~18,000 years ago. In addition, the highest diversity was concentrated in the southern part of the plateau, indicating that this region probably acted as a population refugium during the LGM and the source of a post-LGM expansion within the plateau.
  1. Zhadanov, S. I., Dulik, M. C., Markley, M., Jennings, G. W., Gaieski, J. B., Elias, G., Schurr, T. G., and The Genographic Project Consortium. 2010. Genetic heritage and native identity of the Seaconke Wampanoag tribe of Massachusetts. American Journal of Physical Anthropology 142: 579-589.
  • The biological ancestry of the Seaconke Wampanoag tribe, a group of Native American clans in southern Massachusetts, reflects the genetic consequences of epidemics and conflicts during the 16th century that decimated their population, reducing them from an estimated 12,000 individuals at the beginning of the century to less than 400 at the end. The majority of the paternal and maternal lineages in present-day Seaconke Wampanoag, however, belong to West Eurasian and African lineages, revealing the extensive interactions with people from different ancestries that settled the region during the past four centuries.

2011

  1. Adler, C. J., Haak, W., Donlon, D., Cooper, A., and The Genographic Consortium. 2011. Survival and recovery of DNA from ancient teeth and bones. Journal of Archaeological Science 38: 956-964.
  • The recovery of genetic material from ancient human remains depends on the sampling methods used as well as the environment where the human material was preserved. The results presented in this study quantify the damage caused to ancient DNA by various methods of sampling teeth and bones. The negative impact is minimized if very low drill speeds are used during DNA extraction, increasing both the quantity and quality of material recovered. In addition, the mtDNA content of tooth cementum was five times higher than other commonly used methods, making this component the best place to sample ancient DNA. These conclusions will help to guide future sampling of DNA from ancient material.
  1. Haber, M., Platt, D. E., Badro, D. A., Xue, Y., El-Sibai, M., Ashrafian Bonab, M., Youhanna, S. C., Saade, S., Soria-Hernanz, D. F., Royyuru, A., Wells, R. S., Tyler-Smith, C., Zalloua, P. A., and The Genographic Consortium. 2011. Influences of history, geography, and religion on genetic structure: The Maronites in Lebanon. European Journal of Human Genetics 19: 334-340.
  • Cultural patterns frequently leave genetic traces. The aim of this study was to explore the genetic signature of the establishment of religious communities in a region where some of the most influential world religions originated, using the Y chromosome as an informative male-lineage marker. The analysis shows that the religions in Lebanon were adopted within already distinguishable communities. Differentiation appears to have begun before the establishment of Islam and Christianity, dating to the Phoenician period, and isolation continued during the period of Persian domination. Religious affiliation served to reinforce the genetic signatures of pre-existing population differentiation.
  1. Martínez-Cruz, B., Ziegle, J., Sanz, P., Sotelo, G., Anglada, R., Plaza, S., Comas, D., and The Genographic Consortium. 2011. Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes. Investigative Genetics 2: Article 13.
  • This paper presents a robust and accurate Y-chromosome multiplex assay that can genotype in a single reaction 121 markers distinguishing most of the haplogroups and subhaplogroups observed in European populations. The assay was >99 percent accurate in assigning haplogroups, minimizing sample handling errors that can occur with several independent TaqMan reactions.
  1. Jota, M. S., Lacerda, D. R., Sandoval, J. R., Vieira, P. P. R., Santos-Lopes, S. S., Bisso-Machado, R., Paixão-Cortes, V. R., Revollo, S., Paz-y-Miño, C., Fujita, R., Salzano, F. M., Bonatto, S. L., Bortolini, M. C., Tyler-Smith, C., Santos, F. R., and The Genographic Consortium. 2011. A new subhaplogroup of Native American Y-chromosomes from the Andes. American Journal of Physical Anthropology (published online Sept. 13, 2011.)
  • Almost all Y chromosomes in South America fall into a single haplogroup, Q1a3a. This paper presents a new single nucleotide polymorphism (SNP) in the Q1a3a lineage that is specific to Andean populations, allowing more accurate inferences of the population history of this region. This novel marker is estimated to be ~5,000 years old, consistent with an ancient settlement of the Andean highlands.
  1. Yan, S., Wang, C. C., Li, H., Li, S. L., Jin, L., and The Genographic Consortium. 2011. An updated tree of Y-chromosome Haplogroup O and revised phylogenetic positions of mutations P164 and PK4. European Journal of Human Genetics 19: 1013-1015.
  • Y-chromosome Haplogroup O is the dominant Y-chromosome lineage in East Asians, carried by more than a quarter of all males on the world. This study revises the haplogroup O phylogeny, using several recently discovered markers. The newly generated tree for this haplogroup will lead to a more detailed understanding of the population history of East Asia.
  1. Yang, K., Zheng, H., Qin, Z., Lu, Y., Farina, S. E., Li, S., Jin, L., Li, D., Li, H., and The Genographic Consortium. 2011. Positive selection on mitochondrial M7 lineages among the Gelong people in Hainan. Journal of Human Genetics 56: 253-256.
  • The Gelong people migrated in the last 1,000 years from Guizhou province in southern China to Hainan island (the hottest province in China). The genetic structure of the Gelong people showed a clearly sex-biased pattern of admixture with the indigenous Hainan population (Hlai people), with 30.7 percent of the maternal lineages being of Hainan origin in contrast to 4.9 percent of the paternal lineages. This striking pattern is partially explained through the action of selection on the M7 Hainan autochthonous maternal lineages, leading to their expansion in the admixed population. This may be due to some selective advantage provided by the M7 lineages in the tropical Hainan climate. Future whole mtDNA genome sequencing of these M7 lineages may reveal their functional relevance and the mechanism involved in human adaptation to tropical climates.
  1. Balanovsky, O., Dibirova, K., Dybo, A., Mudrak, O., Frolova, S., Pocheshkhova, E., Haber, M., Platt, D., Schurr, T., Haak, W., Kuznetsova, M., Radzhabov, M., Balaganskaya, O., Druzhinina, E., Zakharova, T., Soria Hernanz, D. F., Zalloua, P., Koshel, S., Ruhlen, M., Renfrew, C., Wells, R. S., Tyler-Smith, C., Balanovska, E., and The Genographic Consortium. 2011. Parallel evolution of genes and languages in the Caucasus region. Molecular Biology and Evolution 28: 2905-2920.
  • The Caucasus region harbors some of the highest linguistic diversity on Earth, leading to the moniker “The Mountain of Languages.” To investigate the forces that may have molded Caucasian linguistic patterns, the Genographic team studied Y-chromosome variation in 1,525 men from 14 populations in the Caucasus. The Y-chromosome lineages found in the Caucasus originated in the Near East and were introduced to the Caucasus in the late Upper Paleolithic or early Neolithic periods. This initial settlement was followed by a high degree of population isolation due to the mountainous terrain. Comparisons between the genetic and linguistic trees showed a striking correspondence between the topology and divergence times for the two, revealing a parallel evolution of genes and languages in the Caucasus in the past few millennia. This high degree of correspondence between genetic and linguistic patterns has not been seen in other regions of the world.
  1. Gaieski, J. B., Owings, A. C., Vilar, M. G., Dulik, M. C., Gaieski, D. F., Gittelman, R. M., Lindo, J., Gau, L., Schurr, T. G., and The Genographic Consortium. 2011. Genetic ancestry and indigenous heritage in a Native American descendant community in Bermuda. American Journal of Physical Anthropology 146: 392-405.
  • Bermuda is an isolated group of islands in the middle of the Atlantic settled during the 17th century by Western Europeans along with African and Native American slaves. The pattern of Y-chromosome and mitochondrial DNA diversity was studied in 111 members of a “native” community on St. David’s Island. Two-thirds of the paternal lineages are of European origin, while two-thirds of the mitochondrial DNA lineages are African. In contrast to other English-speaking communities in the Americas, however, the majority of St. David’s maternal lineages appear to derive from central and southern Africa, regions that historically were controlled by Portuguese slave traders. It is likely that the English settlers of Bermuda obtained slaves from these Portuguese sources. Despite genealogical records and oral traditions indicating significant arrivals of Native Americans as labor force, the proportion of Native American lineages was less than 2 percent on both the paternal and maternal sides. This study gives new insights into the complex history of colonization and migration in the Caribbean.
  1. Cai, X., Qin, Z., Wen, B., Xu, S., Wang, Y., Lu, Y., Wei, L., Wang, C., Li, S., Huang, X., Jin, L., Li, H., and The Genographic Consortium. 2011. Human Migration through bottlenecks from Southeast Asia into East Asia during Last Glacial Maximum revealed by Y chromosomes. PLoS ONE 6: e24282. doi:10.1371/journal.pone.0024282
  • The number and timing of the initial migrations to East Asia remain unresolved. This paper studied the Y-chromosome diversity in Mon-Khmer (MK)- and Hmong-Mien (HM)-speaking populations who are believed to be the source populations of other East Asians. The pattern of diversity for the O3a3b-M7 and O3a3c1-M117 lineages among MK, HM and other East Asian populations suggests an early unidirectional diffusion from Southeast Asia northward into East Asia around the time of the Last Glacial Maximum (~18,000 years ago). The ancestral population sizes of these first colonizers are believed to have gone through drastic reductions due to the barriers imposed by the geographic conditions (mountains and jungle) and the colder climate at the time of the migration. This “serial bottleneck” effect has left a distinctive genetic pattern in the present-day populations of East Asia, revealing their past demographic history.
  1. Melé, M., Javed, A., Pybus, M., Zalloua, P., Haber, M., Comas, D., Netea, M. G., Balanovsky, O., Balanovska, E., Jin, L., Yang, Y., Pitchappan, R. M., Arunkumar, G., Parida, L., Calafell, F., Bertranpetit, J., and The Genographic Consortium. 2011. Recombination gives a new insight in the effective population size and the history of the Old World human populations. Molecular Biology and Evolution (published online Sept. 1, 2011.) doi:10.1093/molbev/msr213
  • The IRiS method (described in paper 12) was used to assess the patterns of recombination on the X chromosome in 30 populations from Africa, Europe and Asia. The results suggest that the ancestors of non-African populations first left Africa in a single coastal migration across the Bad-el-Mandeb strait rather than through the Sinai Peninsula. The method allowed the team to estimate that sub-Saharan ancestral population sizes were four times greater than those in populations outside of Africa, while Indian ancestral sizes were the greatest among Eurasians. These results suggest that Indian populations played a major role in the expansions of modern humans to the rest of the world.
  1. Javed, A., Melé, M., Pybus, M., Zalloua, P., Haber, M., Comas, D., Netea, M. G., Balanovsky, O., Balanovska, E., Jin, l., Yang, Y., Arunkumar, G., Pitchappan, R., Bertranpetit, J., Calafell, F., Parida, L., and The Genographic Consortium. 2011. Recombination networks as genetic markers in a human variation study of the Old World. Human Genetics (first published online Oct. 18, 2011.)
  • An expanded analysis of the recombination dataset published in abbreviated form in paper 24, analyzing three additional populations. The conclusions outlined in paper 24 are bolstered through the more thorough presentation of the results.

2012

  1. Behar DM, Harmant C, Manry J, van Oven M, Haak W, Martinez-Cruz B, Salaberria J, Oyharçabal B, Bauduer F, Comas D, Quintana-Murci L; Genographic Consortium. 2012. The Basque paradigm: genetic evidence of a maternal continuity in the Franco-Cantabrian region since pre-Neolithic times. American Journal of Human Genetics 9;90(3):486-93.
  • This study focus on the maternal genetic diversity of Basques, the last European population to have kept a pre-Indo European language, to increase knowledge of the origins of the Basque people and, more generally, on the role of the Franco-Cantabrian refuge in the post-glacial repopulation of Europe. The maternal ancestry of 908 Basque and non-Basque individuals from the Great Basque Country and adjacent regions were studied plus 420 complete mtDNA genomes within haplogroup H. The results identified six mtDNAhaplogroups autochthonous to the Franco-Cantabrian region and, more specifically, to Basque-speaking populations. Further, expansion of these haplogroups were estimated at ~4,000 ybp  with a separation from the general European gene pool to have happened  ~8,000 ybp predating the Indo-European arrival to the region. Thus, the results clearly support the hypothesis of a partial genetic continuity of contemporary Basques with the indigenous Paleolithic settlers of their homeland.
  1. Martínez-Cruz B, Harmant C, Platt DE, Haak W, Manry J, Ramos-Luis E, Soria-Hernanz DF, Bauduer F, Salaberria J, Oyharçabal B, Quintana-Murci L, Comas D; the Genographic Consortium. Evidence of pre-Roman tribal genetic structure in Basques from uniparentally inherited markers. Molecular Biology and Evolution (published online March 12, 2012) doi: 10.1093/molbev/mss091.
  • Basques have received considerable attention from anthropologists, geneticists and linguists during the last century due to the singularity of their language and to other cultural and biological characteristics. Despite the multidisciplinary efforts performed to address the questions of the origin, uniqueness and heterogeneity of Basques, the genetic studies performed up to now have suffered from a weak study-design where populations are not analyzed in an adequate geographic and population context. To address the former questions and to overcome these design limitations, uniparental genomes (Y chromosome and mitochondrial DNA) of ~900 individuals from 18 populations were analyzed, including those where Basque is currently spoken and surrounding populations where Basque might have been spoken in historical times. Results situate Basques within the western European genetic landscape, although with less external influences than other Iberians and French populations. In addition, the genetic heterogeneity and structure observed in the Basque region results from pre-Roman tribal structure related to geography and is linked to the increased complexity of emerging societies during the Bronze Age. The rough overlap of tribal and current dialect limits supports the notion that the environmental diversity in the region has played a recurrent role in cultural differentiation and ethnogenesis at different time periods.
  1. Kang, L., Lu, Y., Wang, C., Hu, K., Chen, F., Liu, K., Li, S., Jin, L., Li, H., and The Genographic Consortium. 2012. Y-chromosome O3 Haplogroup diversity in Sino-Tibetan populations reveals two migration routes into the Eastern Himalayas. Annals of Human Genetics 76: 92–99.
  • This paper further explores the question of how Himalayas was populated by studying the genetic diversity of the paternal lineages of two ethnic groups from the eastern Himalayas: the Luoba and Deng. These two Sino-Tibetan speaking groups exhibited a distinct genetic composition indicating different genetic origins. The paternal diversity of the Louba people indicates past gene flow from Tibetans as well as from western and north Eurasian people. In contrast, Deng exhibited lineages similar to most of Sino-Tibetans from the east. The overall lowest diversity observed in the eastern Himalayas suggests that this area was the end point of two migratory routes of Sino-Tibetans from north China around 2,000-3,000 years ago. These date estimates also agrees with the historical records.
  1. Lu, Y., Wang, C., Qin, Z., Wen, B., Farina, S. E., Jin, L., Li, H., and The Genographic Consortium. 2012. Mitochondrial origin of the matrilocal Mosuo people in China. Mitochondrial DNA 23: 13–19
  • The Mosuo people currently live around the Lugu Lake on the border of the Yunan and Sichuan provinces of China and they are the last matrilocal population in the main land of the country. To investigate the maternal history of this ethnic group, partial genetic sequences of the mitochondria (a maternally inherited genome) were studied among Mosuo people and other larger surrounding ethnic groups. Groups with matrilocal traditions are expected to exhibited a lower mitochondrial genetic diversity because the movement of these genomes are reduced since woman remain within families after marriage. However, the results presented here did not reflect these expectations indicating that Mouso may have started practicing matrilocality long time ago, at least after the Paleolithic Age. In contrast to previous studies that showed a clear relationship between Mouso and Naxi people based on just mtDNA haplogroup frequencies, the network analyses presented here indicated clear clusters of individual sequences between Mouso and Pumi lineages. The genetic resemblance between these two group are concordant with other evidences from cultural and language studies. These results indicate that simply comparing haplogroups frequencies among ethnic groups may lead to erroneous conclusions and analyses comparing mtDNA sequences are better suitable for exploring genetic relationship among ethnic groups.
  1. Haber M, Platt DE, Ashrafian Bonab M, Youhanna SC, Soria-Hernanz DF, Martínez-Cruz B, Douaihy B, Ghassibe-Sabbagh M, Rafatpanah H, Ghanbari M, Whale J, Balanovsky O, Wells RS, Comas D, Tyler-Smith C, Zalloua PA; The Genographic Consortium. 2012. Afghanistan’s Ethnic Groups Share a Y-Chromosomal Heritage Structured by Historical Events. PLoS ONE 7(3): e34288. doi:10.1371/journal.pone.0034288
  • This study focus on how Afghanistan’s ethnic groups relate to each others and with other populations from neighboring countries. The results presented indicated that major genetic differences among Afghanistan’s ethnic groups are relatively recent. The different modern ethnic groups share a genetic heritage probably formed during the Neolithic in the founding of the early farming communities. However, differentiation among the ethnic groups likely started during the Bronze Age driven by the establishment of the first civilizations. Later migrations and invasions to the region, gave the Afghans a unique genetic diversity in Central Asia.
  1. Schurr, T. G., Dulik, M. C., Owings, A. C., Zhadanov, S. I., Gaieski, J. B., Vilar, M. G., Ramos, J., Moss, M. B., Natkong, F. and The Genographic Consortium. 2012. Clan, language, and migration history has shaped genetic diversity in Haida and Tlingit populations from Southeast Alaska. American Journal of Physical Anthropology. (published online May 1, 2012) doi: 10.1002/ajpa.22068.
  • This manuscript gives new insights about the genetics of the linguistically distinctive Haida and Tlingit tribes of Southeast Alaska. More espcifically, this paper study the role that Southeast Alaska may have played in the early colonization of the Americas; the genetic relationships of Haida and Tlingit to other indigenous groups in Alaska and Canada; the relationship between linguistic and genetic data for populations assigned to the Na-Dene linguistic family; the possible influence of matrilineal clan structure on patterns of genetic variation in Haida and Tlingit populations; and the impact of European entry into the region on the genetic diversity of these indigenous communities. The analysis indicates that, while sharing a ‘northern’ genetic profile, the Haida and the Tlingit are genetically distinctive from each other.  In addition, Tlingit groups themselves differ across their geographic range, in part due to interactions of Tlingit tribes with Athapaskan and Eyak groups to the north.  The data also reveal a strong influence of maternal clan identity on mtDNA variation in these groups, as well as the significant influence of non-native males on Y-chromosome diversity.  These results yield new details about the histories of the Haida and Tlingit tribes in this region.
  1. Dulik, M. C., Owings, A. C., Zhadanov, S. I., Gaieski, J. B., Vilar, M. G., Schurr, T. G., and The Genographic Consortium. 2012. Y-chromosome analysis of native North Americans reveals new paternal lineages and genetic differentiation between Eskimo-Aleut and Dene speaking populations. Accepted for publication in April in PNAS.
  • The genetic origins of the linguistically diverse Native Americans and when they reached the Americas are questions that have been explored during the last several decades. This study provides new information to these questions by increasing the number of populations sampled and the genetic resolution used in the analyses Here, it is tested whether there is any correlation between genetic diversity from paternally inherited Y-chromosomes and native populations speaking the two distinctive linguistic families: Eskimo-Aleut and Na-Dene. The results indicate that the Y chromosome genetic diversity among the first Native American was greater than previously shown in other publications. In addition, the Eskimo-Aleut and Na-Dene speaking populations showed clear genetic differences between then.  The disparities in language, culture and genetic diversity between these two populations likely reflect the outcome of two migrations that happened after the initial settlement of people into the Americas.
  1. Martinez-Cruz B, Ioana M, Calafell F, Arauna LR, Sanz P, Ionescu R, Boengiu S, Kalaydjieva L, Pamjav H, Makukh H, Plantiga T, van der Meer JWM, Comas D, Netea M, The Genographic Consortium. 2012. Y-chromosome analysis in individuals bearing the Basarab name of the first dynasty of Wallachian kings. PLoS ONE 7(7): e41803
  • The most famous Transylvanian prince is Vlad III from the Basarab royal dynasty, also commonly known as Dracula. The ethnic origins of the Basarab is intensively debated among historians and it is unclear of whether they are descendants of the Cuman people (an admixed Turkic people that reached Romania from the East in the 11th century) or of Vlach people (local Romanians). This paper investigated the Y chromosome of 29 Romanian men carrying the surname Basarab and in order to identify their genetic origin the data was compared with four Romanian and other surrounding populations. Different Y-chromosome haplogroups were found within the individuals bearing the Basarab name, indicating that not all these individuals can be direct biological descendants of the Basarab dynasty. In addition, all these haplogroups are common in Romania and other Central and Eastern European populations. The Basarab group exhibited closer genetic distances with other Romanian populations. These results together with the absence of Eastern Asian paternal lineages in the Basarab men can be interpreted as a lack of evidence for a Cuman origin of this royal dynasty, although it cannot be positively ruled out. As a final conclusion, it seems that the Basarab dynasty was successful in spreading its name beyond the spread of its genes.
  1. Rebala K, Martínez-Cruz B, Tönjes A, Kovacs P, Stumvoll M, Lindner I, Büttner A, Wichmann H-E, Siváková D, Soták M, Quintana-Murci L, Szczerkowska Z, Comas D, The Genographic Consortium. 2012. Contemporary paternal genetic landscape of Polish and German populations: from early medieval Slavic expansion to post-World War II resettlements. European Journal of Human Genetics 21(4): 415-422
  • One of the most outstanding phenomena in the Y-chromosomal diversity in Europe concerns the sharp genetic border identified between the ethnically /linguistically defined Slavic (from Poland) and German populations (from Germany). The Polish paternal lineages also reveal great degree of homogeneity in spite of a relatively large geographic area seized by the Polish state. Two main explanations have been proposed to explain the phenomena: (i) Massive human resettlements during and shortly after the World War II, and (ii) an early medieval Slavic migrations that displayed previous genetic heterogeneity. In order to answer these questions, 1,156 individuals from several Slavic and German populations were analyzed, including Polish pre-war regional populations and an autochthonous Slavic population from Germany. This study demonstrates for the first time that the Polish paternal lineages were unevenly distributed within the country before the forced resettlements of millions of people during and shortly after the WWII. Finally, the coalescence analyses support hypothesis that the early medieval Slavic expansion in Europe was a demographic event rather than solely a linguistic spread of the Slavic language.
  1. Arunkumar G, Soria-Hernanz DF, Kavitha VJ, Arun VS, Syama A, Ashokan KS, Gandhirajan KT, Vijayakumar K, Narayanan M, Jayalakshmi M, Ziegle JS, Royyuru AK, Parida L, Wells RS, Renfrew C, Schurr TG, Smith CT, Platt DE, Pitchappan R; Genographic Consortium. 2012. Population differentiation of southern Indian male lineages correlates with agricultural expansions predating the caste system. PLoS ONE. 7(11): e50269
  • Previous studies that pooled Indian populations from a wide variety of geographical locations, have obtained contradictory conclusions about the processes of the establishment of the Varna caste system. This study investigates the origin of the caste system by genotyping 1,680 Y chromosomes representing 12 tribal and 19 non-tribal (caste) populations from the Dravidian-speaking Tamil Nadu state in the southernmost part of India. 81% of Y chromosome were autochthonous Indian haplogroups (H-M69, F-M89, R1a1-M17, L1-M27, R2-M124, and C5-M356; 81% combined) with a shared genetic heritage dating back to the late Pleistocene (10-30 Kya). Results show a strong evidence for genetic structure, and coalescent analyses suggest that the stratification was established 4-6 thousand years ago, with little admixture took place during the last several millennia. The overall Y-chromosomal patterns, the time depth of population diversifications and the period of differentiation are best explained by the emergence of agricultural technology in South Asia. These results highlight the utility of detailed local genetic studies within India, without prior assumptions about the importance of Varna rank status for population grouping, to obtain new insights into the relative influences of past demographic events for the population structure of the whole of modern India.

2013

  1. Badro DA, Douaihy B, Haber M, Youhanna SC, Salloum A, Ghassibe-Sabbagh M, Johnsrud B, Khazen G, Matisoo-Smith E, Soria-Hernanz DF, Wells RS, Tyler-Smith C, Platt DE, Zalloua PA, The Genographic Consortium. 2013. Y-chromosome and mtDNA genetics reveal significant contrasts in affinities of Modern Middle Eastern populations with European and African populations. PLoS ONE 8(1):e54616
  • The Middle East was a funnel of human expansion out of Africa, a staging area for the Neolithic Agricultural Revolution, and the home to some of the earliest world empires. In addition, post LGM expansions into the region and subsequent population movements have created a striking genetic mosaic in the region. In this study 5,174 mtDNA and 4,658 Y-chromosome samples were investigated. Lebanon’s mtDNA showed a very strong association to Europe, while Yemen shows very strong affinity with Egypt and North and East Africa. Previous Y-chromosome results showed a Levantine coastal-inland contrast marked by Y-haplogroups J1 and J2, and a very strong North African component was evident throughout the Middle East. Neither of these patterns were observed in the mtDNA. While J2 has penetrated into Europe, the pattern of Y-chromosome diversity in Lebanon does not show the widespread affinities with Europe, as indicated by the mtDNA data. Lastly, while each population shows evidence of historic expansions that now define the Middle East, Africa, and Europe, most Middle Eastern populations show distinctive mtDNA and Y-haplogroup characteristics that suggest long standing settlements with relatively little impact from other populations.
  1. WANG, C.-C., YAN, S., QIN, Z.-D., LU, Y., DING, Q.-L., WEI, L.-H., LI, S.-L., YANG, Y.-J., JIN, L., LI, H. and the Genographic Consortium. 2013. Late Neolithic expansion of ancient Chinese revealed by Y chromosome haplogroup O3a1c-002611. Journal of Systematics and Evolution, 51: 280–286.
  • Y chromosome haplogroup O3-M122 is the most prevalent haplogroup in East Asia, and provides an ideal tool for dissecting primary dispersals of the East Asians. In this study, we identified 508 individuals with haplogroup O3a1c-002611 out of 7801 males from 117 East and Southeast Asian populations, typed at two newly discovered downstream Y-SNP markers and ten commonly used Y-STRs. STR diversity shows a general south-to-north decline, which is consistent with the prehistorically northward migration of the other O3-M122 lineages. The northward migration of haplogroup O3a1c-002611 started about 13 thousand years ago (KYA). The expansions of subclades F11 and F238 in ancient Han Chinese began about 5-7 KYA immediately after the separation between the ancestors of the Han Chinese and Tibeto-Burman.
  1. DENG, Q.-Y., WANG, C.-C., WANG, X.-Q., WANG, L.-X., WANG, Z.-Y., WU, W.-J., LI, H. and the Genographic Consortium. 2013. Genetic affinity between the Kam-Sui speaking Chadong and Mulam people. Journal of Systematics and Evolution, 51: 263–270
  • The origins of Kam-Sui speaking Chadong and Mulam people have been controversial subjects in ethnic history studies and other related fields. Here, we studied Y chromosome (40 informative SNPs and 17 STRs in a non-recombining region) and mtDNA (hypervariable segment I and coding region single nucleotide polymorphisms) diversities in 50 Chadong and 93 Mulam individuals. The Y chromosome and mtDNA haplogroup components and network analyses indicated that both Chadong and Mulam originated from the admixture between surrounding populations and the indigenous Kam-Sui populations. The newly found Chadong is more closely related to Mulam than to Maonan, especially in the maternal lineages.
  1. LI, D.-N., WANG, C.-C., YANG, K., QIN, Z.-D., LU, Y., LIN, X.-J., LI, H. and the Genographic Consortium. 2013. Substitution of Hainan indigenous genetic lineage in the Utsat people, exiles of the Champa kingdom. Journal of Systematics and Evolution, 51: 287–294
  • The Utsat people do not belong to one of the recognized ethnic groups in Hainan, China. In the present study, we typed paternal Y chromosome and maternal mitochondrial (mt) DNA markers in 102 Utsat people to gain a better understanding of the genetic history of this population. High frequencies of the Y chromosome haplogroup O1a*-M119 and mtDNA lineages D4, F2a, F1b, F1a1, B5a, M8a, M*, D5, and B4a exhibit a pattern similar to that seen in neighboring indigenous populations. Cluster analyses (principal component analyses and networks) of the Utsat, Cham, and other ethnic groups in East Asia indicate that the Utsat are much closer to the Hainan indigenous ethnic groups than to the Cham and other mainland southeast Asian populations. These findings suggest that the origins of the Utsat likely involved massive assimilation of indigenous ethnic groups. During the assimilation process, the language of Utsat has been structurally changed to a tonal language; however, their Islamic beliefs may have helped to keep their culture and self-identification.
  1. Der Sarkissian C, Balanovsky O, Brandt G, Khartanovich V, Buzhilova A, Koshel S, Zaporozhchenko V, Gronenborn D, Moiseyev V, Kolpakov E, Shumkin V, Alt KW, Balanovska E, Cooper A, Haak W, The Genographic Consortium. 2013. Ancient DNA reveals prehistoric gene-flow from Siberia in the complex human population history of North East Europe. PLoS Genetics 9(2): e1003296
  • Archaeological, anthropological, and genetic research of Northeastern European populations have revealed a series of influences from Western and Eastern Eurasia. While genetic data from modern-day populations is commonly used to make inferences about origins and past migrations, ancient DNA provides a powerful tool by giving a snapshot of the past genetic diversity. This study generated and analyzed 34 mitochondrial genotypes from the skeletal remains of three Mesolithic and the Early Metal Age (7,500 and 3,500 years ago) sites in northwest Russia. Comparisons of genetic data from ancient and modern-day populations revealed significant changes in the makeup of North East Europeans through time. Mesolithic foragers showed high frequencies and diversity of haplogroup U (U2e, U4, U5a), commonly observed in hunter-gatherers from Iberia to Scandinavia. In contrast, the presence of mitochondrial DNA haplogroups C, D, and Z in Early Metal Age individuals suggested genetic influx from central/eastern Siberia. This genetic dissimilarities between prehistoric and modern-day North East Europeans/Saami suggests a strong influence of post-Mesolithic migrations from Western Europe and subsequent population replacement/extinctions. This work demonstrated how ancient DNA can improve our understanding of human population movements across Eurasia.
  1. Brotherton P, Haak W, Templeton J, Brandt G, Soubrier J, Jane Adler C, Richards SM, Sarkissian CD, Ganslmeier R, Friederich S, Dresely V, van Oven M, Kenyon R, Van der Hoek MB, Korlach J, Luong K, Ho SY, Quintana-Murci L, Behar DM, Meller H, Alt KW, Cooper A, The Genographic Consortium. 2013. Neolithic mitochondrial haplogroup H genomes and the genetic origins of Europeans. Nature Communications 4:1764
  • Haplogroup H dominates present-day Western European mitochondrial DNA variability (>40%), yet was less common (~19%) among Early Neolithic farmers (~5450 BC) and virtually absent in Mesolithic hunter-gatherers. This project investigated maternal population history of modern Europeans by sequencing 39 complete haplogroup H mitochondrial genomes from ancient remains; and comparing this ‘real-time’ genetic data with cultural changes taking place between the Early Neolithic (~5450 BC) and Bronze Age (~2200 BC) in Central Europe. Results revealed that the current diversity and distribution of haplogroup H were largely established by the Mid Neolithic (~4000 BC), but with substantial genetic contributions from later pan-European cultures such as the Bell Beakers expanding out of Iberia in the Late Neolithic (~2800 BC). Newly dated haplogroup H genomes enabled the reconstruction of the evolutionary history of the haplogroup, and revealed a mutation rate 45% higher than previous estimates.
  1. LI, D.-N., WANG, C.-C., LU, Y., QIN, Z.-D., YANG, K., LIN, X.-J., LI, H. and the Genographic Consortium. 2013. Three phases for the early peopling of Hainan Island viewed from mitochondrial DNA. Journal of Systematics and Evolution, 51: 671–680
  • Hainan, an island linking mainland East Asia and Southeast Asia, lay in one of the routes of early migration to East Asia. Here, we have analyzed mitochondrial DNA control-region and coding-region sequence variations in 566 Hlai individuals from all five subgroups, Ha, Gei, Zwn, Moifau, and Jiamao. Our results suggest three phases for the peopling of Hainan. The first phase represents the initial settlement of the island as part of the African dispersal approximately 50 000 years ago. The second phase reflects colonization events from mainland Asia before the Last Glacial Maximum, which was recorded by wide distributed lineages, such as F*, B4a, and D4a.The third phase reflects population expansions under lineages F1b, M7b, and R9b after the Last Glacial Maximum and Neolithic migrations in and out of Hainan Island. Selection also started to play a role during the last phase.
  1. Elhaik E, Greenspan E, Staats S, Krahn T, Tyler-Smith C, Xue Y, Tofanelli S, Francalacci P, Cucca F, Pagani L, Jin L, Li H, Schurr TG, Greenspan B, Spencer Wells R, The Genographic Consortium. 2013. The GenoChip: a new tool for genetic anthropology. Genome Biology & Evolution 5(5): 1021-1031
  • The Genographic Project is an international effort aimed at charting human migratory history. The first phase of the project was focused on haploid DNA markers (Y-chromosome and mtDNA), while the current phase focuses on markers from across the entire genome using the newly created GenoChip. GenoChip was designed to enable higher resolution research into outstanding questions in genetic anthropology. It includes ancestry informative markers obtained for over 450 human populations, an ancient human (Saqqaq), and two archaic hominins (Neanderthal and Denisovan) and it was designed to identify all known Y-chromosome and mtDNA haplogroups. The chip was also carefully vetted to avoid inclusion of medically relevant markers. To demonstrate its capabilities, we compared the FST distributions of GenoChip SNPs to those of two commercial arrays. Although all arrays yielded similarly shaped FST distributions, the GenoChip autosomal and X-chromosomal distributions had the highest mean FST, attesting to its ability to discern subpopulations. In summary, the GenoChip is a dedicated genotyping platform for genetic anthropology. With an unprecedented number of approximately 12,000 Y-chromosomal and approximately 3,300 mtDNA SNPs and over 130,000 autosomal and X-chromosomal SNPs with no health, medical, or phenotypic relevance, the GenoChip is a useful tool for genetic anthropology and human population genetics.
  1. Boattini A, Martinez-Cruz B, Sarno S, Harmant C, Useli A, Sanz P, Yang-Yao D, Manry J, Ciani G, Luiselli D, Quintana-Murci L, Comas D, Pettener D; The Genographic Consortium. 2013. Uniparental markers in Italy reveal a sex-biased genetic structure and different historical strata. PLoS ONE 8(5): e65441
  • Italy played an important role in the history of human settlements and movements of Southern Europe and the Mediterranean. Populated since Paleolithic times, the complexity of human movements during the Neolithic, the Metal Ages and the most recent history of the two last millennia, shaped the pattern of the modern Italian genetic structure. With the aim of disentangling this pattern, this project analyzed the haploid markers in ∼900 individuals from across the Italian peninsula, Sardinia and Sicily. Results show a sex-biased pattern, indicating different demographic histories for males and females. Besides the genetic outlier position of Sardinians, a North West-South East Y-chromosome structure appeared through continental Italy, likely a result of historical and demographic events. In contrast, mitochondrial (maternal) diversity is distributed homogeneously in accordance with older pre-historic events, as was the presence of an Italian Refugium during the last glacial period in Europe.
  1. Sandoval JR, Lacerda DR, Jota MS, Salazar-Granara A, Vieira PP, Acosta O, Cuellar C, Revollo S, Fujita R, Santos FR, The Genographic Consortium. 2013. The genetic history of indigenous populations of the Peruvian and Bolivian Altiplano: the legacy of the Uros. PLoS ONE 8(9): e73006
  • Since pre-Columbian times, different cultures established themselves around the Titicaca and Poopo Lakes. Yet by the time of Spanish colonization, the Inca Empire and the Aymara and Quechua languages were dominant in the region. This study focused on the pre-Columbian history of the Altiplano populations, particularly the Uros, which claim to be directly descend from the first settlers of the Andes. Results indicate that the Uros populations stand out among others in the Altiplano, while appearing more closely related to the Aymara and Quechua from Lake Titicaca and surrounding regions, than to the Amazon Arawaks. Moreover, the Uros populations from Peru and Bolivia are genetically differentiated from each other, indicating a high heterogeneity in this ethnic group. Lastly, the results support the distinctive ancestry for the Uros populations of Peru and Bolivia, likely derived from ancient Andean lineages, but further complicated by a partial replacement during more recent farming expansion, and the establishment of complex civilizations in the Andes, such as the Inca.
  1. Brandt G, Haak W, Adler CJ, Roth C, Szécsényi-Nagy A, Karimnia S, Möller-Rieker S, Meller H, Ganslmeier R, Friederich S, Dresley V, Nicklish N, Pickrell JK, Siroko F, Reich D, Cooper A, Alt KW, The Genographic Consortium 2013. Ancient DNA Reveals Key Stages in the Formation of Central European Mitochondrial Genetic Diversity. Science 342, no.6155: 257-261.
  • Genographic project scientists, in collaboration with archeologists from Germany, successfully sequenced and analyzed DNA from 364 individuals that lived in Central Europe between 5,500 and 1,500 BC. What they found was that the shift in the frequency of DNA lineages closely matched the changes and appearances of new Central European cultures across time. In other words, the people who lived in Central Europe 7,000 years ago had different DNA lineages than those that lived there 5,000 years ago, and again different to those that lived 3,500 years ago. Central Europe was dynamic place during the Bronze age, and the genetic composition of the people that lived there demonstrates that. Ultimately, Central Europe is a melting pot of genetic lineages from different prehistoric cultures that lived there at different periods of time, each new one partially replacing the one before it.

2014

  1. Clarke AC, Prost S, Stanton JL, W, White TJ, Kaplan ME, Matisoo-Smith EA, and The Genographic Consortium. 2014. From cheek swabs to consensus sequences: an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes. BMC Genomics 15:68
  • Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA genomes (of humans and other animals). Despite the increasing use of NGS technologies and a better appreciation of their importance in answering biological questions, there remain significant obstacles to the successful implementation of NGS-based projects, especially for new users. Here we present an ‘A to Z’ protocol for obtaining complete human mitochondrial (mtDNA) genomes – from DNA extraction to consensus sequence. Although designed for use on humans, this protocol could also be used to sequence small, organellar genomes from other species, and also nuclear loci. This protocol includes DNA extraction, PCR amplification, fragmentation of PCR products, barcoding of fragments, sequencing using the 454 GS FLX platform, and a complete bioinformatics pipeline (primer removal, reference-based mapping, output of coverage plots and SNP calling). All steps in this protocol are designed to be straightforward to implement, especially for researchers who are undertaking next-generation sequencing for the first time. The molecular steps are scalable to large numbers (hundreds) of individuals and all steps post-DNA extraction can be carried out in 96-well plate format. Also, the protocol has been assembled so that individual ‘modules’ can be swapped out to suit available resources.
  1. Elhaik E, Tatarinova T, Chebotarev D, Piras, IS, Calo CM, De Montis A, Atzori M, Marini M, Tofanelli S, Francalacci P, et al. 2014. Geographic population structure analysis of worldwide human populations infers their biogeographical origins. Nature Communications 5:3513
  • Here we describe the Geographic Population Structure (GPS) algorithm and demonstrate its accuracy with three data sets using 40,000–130,000 SNPs. GPS placed 83% of worldwide individuals in their country of origin. Applied to over 200 Sardinians villagers, GPS placed a quarter of them in their villages and most of the rest within 50 km of their villages. GPS’s accuracy and power to infer the biogeography of worldwide individuals down to their country or, in some cases, village, of origin, underscores the promise of admixture-based methods for biogeography and has ramifications for genetic ancestry testing.
  1. Sarkissian C, Brotherton P, Balanovsky O, Templeton JEL, Llamas B, Soubrier J, Moiseyev V, Khartanovich V, Cooper A, Haak W, The Genographic Consortium. (2014) Mitochondrial Genome Sequencing in Mesolithic North East Europe Unearths a New Sub-Clade within the Broadly Distributed Human Haplogroup C1. PLoS ONE 9(2): e87612. doi:10.1371/journal.pone.0087612
  • The human mitochondrial haplogroup C1 has a broad global distribution but is extremely rare in Europe today. Recent ancient DNA evidence has demonstrated its presence in European Mesolithic individuals. Three individuals from the 7,500 year old Mesolithic site of Yuzhnyy Oleni Ostrov, Western Russia, could be assigned to haplogroup C1 based on mitochondrial hypervariable region I sequences. In order to obtain high-resolution data and shed light on the origin of this European Mesolithic C1 haplotype, we target-enriched and sequenced the complete mitochondrial genome of one Yuzhnyy Oleni Ostrov C1 individual. The updated phylogeny of C1 haplogroups indicated that the Yuzhnyy Oleni Ostrov haplotype represents a new distinct clade, provisionally coined “C1f”. No haplotype closely related to the C1f sequence could be found in the large current database of ancient and present-day mitochondrial genomes. Hence, we have discovered past human mitochondrial diversity that has not been observed in modern-day populations so far.
  1. Vilar, M. G., Melendez, C., Sanders, A. B., Walia, A., Gaieski, J. B., Owings, A. C., Schurr, T. G. and The Genographic Consortium (2014) Genetic diversity in Puerto Rico and its implications for the peopling of the Island and the West Indies. Am. J. Phys. Anthropol., 155: 352–368
  • Puerto Ricans are genetic descendants of pre-Columbian peoples, as well as peoples of European and African descent through 500 years of migration to the island. To infer these patterns of pre-Columbian and historic peopling of the Caribbean, we characterized genetic diversity in 326 individuals from the southeastern region of Puerto Rico and the island municipality of Vieques. We sequenced the mitochondrial DNA (mtDNA) control region of all of the samples and the complete mitogenomes of 12 of them to infer their putative place of origin. In addition, we genotyped 121 male samples for 25 Y-chromosome single nucleotide polymorphism and 17 STR loci. Approximately 60% of the participants had indigenous mtDNA haplotypes (mostly from haplogroups A2 and C1), while 25% had African and 15% European haplotypes. None of the male participants had indigenous Y-chromosomes, with 85% of them instead being European/Mediterranean and 15% sub-Saharan African in origin. These results attest to the distinct, yet equally complex, pasts for the male and female ancestors of modern day Puerto Ricans.

2015

  1. Kushniarevich A, Utevska O, Chuhryaeva M, Agdzhoyan A, Dibirova K, Uktveryte I et al. (2015) Genetic Heritage of the Balto-Slavic Speaking Populations: A Synthesis of Autosomal, Mitochondrial and Y-Chromosomal Data. PLoS ONE 10(9): e0135820
  • Here, we characterize genetic variation in all extant ethnic groups speaking Balto-Slavic languages by analyzing mitochondrial DNA (n = 6,876), Y-chromosomes (n = 6,079) and genome-wide SNP profiles (n = 296), within the context of other European populations. We also reassess the phylogeny of Slavic languages within the Balto-Slavic branch of Indo-European. We find that genetic distances among Balto-Slavic populations, based on autosomal and Y-chromosomal loci, show a high correlation (0.9) both with each other and with geography, but a slightly lower correlation (0.7) with mitochondrial DNA and linguistic affiliation. The data suggest that genetic diversity of the present-day Slavs was predominantly shaped in situ, and we detect two different substrata: ‘central-east European’ for West and East Slavs, and ‘south-east European’ for South Slavs. A pattern of distribution of segments identical by descent between groups of East-West and South Slavs suggests shared ancestry or a modest gene flow between those two groups, which might derive from the historic spread of Slavic people
  1. ArunKumar, GaneshPrasad, Tatiana V. Tatarinova, Jeff Duty, Debra Rollo, Adhikarla Syama, Varatharajan Santhakumari Arun, Valampuri John Kavitha et al (2015) Genome-wide signatures of male-mediated migration shaping the Indian gene pool.” Journal of human genetics. Accepted online May 21, 2105
  • Multiple questions relating to contributions of cultural and demographical factors in the process of human geographical dispersal remain largely unanswered. India, a land of early human settlement and the resulting diversity is a good place to look for some of the answers. In this study, we explored the genetic structure of India using a diverse panel of 78 males genotyped using the GenoChip. Their genome-wide single-nucleotide polymorphism (SNP) diversity was examined in the context of various covariates that influence Indian gene pool. Admixture analysis of genome-wide SNP data showed high proportion of the Southwest Asian component in all of the Indian samples. Hierarchical clustering based on admixture proportions revealed seven distinct clusters correlating to geographical and linguistic affiliations. Convex hull overlay of Y-chromosomal haplogroups on the genome-wide SNP principal component analysis brought out distinct non-overlapping polygons of F*-M89, H*-M69, L1-M27, O2a-M95 and O3a3c1-M117, suggesting a male-mediated migration and expansion of the Indian gene pool. Lack of similar correlation with mitochondrial DNA clades indicated a shared genetic ancestry of females. We suggest that ancient male-mediated migratory events and settlement in various regional niches led to the present day scenario and peopling of India.
  1. Arunkumar, GaneshPrasad, Lan‐Hai Wei, Valampuri John Kavitha, Adhikarla Syama, Varatharajan Santhakumari Arun, Surendra Sathua, Raghunath Sahoo et al. (2015) A late Neolithic expansion of Y chromosomal haplogroup O2a1‐M95 from east to west. Journal of Systematics and Evolution (2015). Accepted online March 31, 2015
  • The origin and dispersal of Y-Chromosomal haplogroup O2a1-M95, distributed across the Austro Asiatic speaking belt of East and South Asia, are yet to be fully understood. Various studies have suggested either an East Indian or Southeast Asian origin of O2a1-M95. We addressed the issue of antiquity and dispersal of O2a1-M95 by sampling 8748 men from India, Laos, and China and compared them to 3307 samples from other intervening regions taken from the literature. Analyses of haplogroup frequency and Y-STR data on a total 2413 O2a1-M95 chromosomes revealed that the Laos samples possessed the highest frequencies of O2a1-M95 (74% with >0.5) and its ancestral haplogroups (O2*-P31, O*-M175) as well as a higher proportion of samples with 14STR-median haplotype (17 samples in 14 populations), deep coalescence time (5.7 ± 0.3 Kya) and consorted O2a1-M95 expansion evidenced from STR evolution. All these suggested Laos to carry a deep antiquity of O2a1-M95 among the study regions. A serial decrease in expansion time from east to west: 5.7 ± 0.3 Kya in Laos, 5.2 ± 0.6 in Northeast India, and 4.3 ± 0.2 in East India, suggested a late Neolithic east to west spread of the lineage O2a1-M95 from Laos.
  1. Martínez-Cruz, Begoña, Isabel Mendizabal, Christine Harmant, Rosario de Pablo, Mihai Ioana, Dora Angelicheva, Anastasia Kouvatsi et al. (2015) Origins, admixture and founder lineages in European Roma. European Journal of Human Genetics. Accepted online September 16, 2015
  • The Roma, also known as ‘Gypsies’, represent the largest and the most widespread ethnic minority of Europe. We performed a high-resolution study of the uniparental genomes of 753 Roma and 984 non-Roma hosting European individuals. Roma groups show lower genetic diversity and high heterogeneity compared with non-Roma samples as a result of lower effective population size and extensive drift, consistent with a series of bottlenecks during their diaspora. We found a set of founder lineages, present in the Roma and virtually absent in the non-Roma, for the maternal (H7, J1b3, J1c1, M18, M35b, M5a1, U3, and X2d) and paternal (I-P259, J-M92, and J-M67) genomes. This lineage classification allows us to identify extensive gene flow from non-Roma to Roma groups, whereas the opposite pattern, although not negligible, is substantially lower (up to 6.3%). Finally, the exact haplotype matching analysis of both uniparental lineages consistently points to a Northwestern origin of the proto-Roma population within the Indian subcontinent.
  1. Jada Benn Torres, Miguel G. Vilar, Gabriel Torres, Jill B. Gaieski, Ricardo Bharath Hernandez, Zoila E. Browne, Marlon Stevenson, Wendell Walters, Theodore G. Schurr, and The Genographic Consortium (2015) Genetic Diversity in the Lesser Antilles and Its Implications for the Settlement of the Caribbean Basin. PLoS One. Accepted for publication October 1, 2015
  • Historical discourses about the Caribbean often chronicle West African and European influence to the general neglect of indigenous people’s contributions to the contemporary region. Consequently, demographic histories of Caribbean people prior to and after European contact are not well understood.  Although archeological evidence suggests that the Lesser Antilles were populated in a series of northward and eastern migratory waves, many questions remain regarding the relationship of the Caribbean migrants to other indigenous people of South and Central America and changes to the demography of indigenous communities post-European contact.  To explore these issues, we analyzed mitochondrial DNA and Y-chromosome diversityin 12 unrelated individuals from the First Peoples Community in Arima, Trinidad, and 43 unrelated Garifuna individuals residing in St. Vincent.  In this community-sanctioned research, we detected maternal indigenous ancestry in 42% of the participants, with the remainder having haplotypes indicative of African and South Asian maternal ancestry.  Analysis of Y-chromosome variation revealed paternal indigenous American ancestry indicated by the presence of haplogroup Q-M3 in 28% of the male participants from both communities, with the remainder possessing either African or European haplogroups.  This finding is the first report of indigenous American paternal ancestry among indigenous populations in this region of the Caribbean.  Overall, this study illustrates the role of the region’s first peoples in shaping the genetic diversity seen in contemporary Caribbean populations.

Family Tree DNA Thanksgiving Week Sale Coupons

2015 ftdna week 2

Week 2 of Family Tree DNA’s sale is here.  Existing customers should receive an e-mail announcing their weekly coupon, or you can view your coupon by signing in to your account and looking for the Mystery Reward featured prominently at the top of your account.2015 Mystery Gift

Coupons can be used in addition to the sale prices, shown below, for products.

2015 ftdna sale prices

Please list your coupon discounts available, if you’re not going to use them, for others to use in the comments of each week’s blog article about the sale and sharing.  You can also request something specific.

Here are a few from accounts that I manage.  I used 4 coupons myself last week – so Happy Holidays to me and my ancestors I’m going to discover a little more about. If you’ve been wanting to test a family member or upgrade, now might be a good time.

Each coupon can only be used once (so first come, first serve on shared codes) and expires the following Monday when the next coupon is issued.

Coupon Type Discount Code
$5 off Y37, Y67 or Y111 R8SHOBHCDNS
$5 off Y37, Y67 or Y111 R8Q4VRCGLY9
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$25 off Big Y R816GSLS0IC
$5 off mtDNA R86VDXCM6SF
$10 off mtDNA R83J02G4021
$10 off mtDNA R8MAFLM4MY4
$5 off Family Finder R8FKW1Y6UAV

Click here to order or upgrade tests and redeem coupons.

Phebe (c1747-c1803), Is Jotham Brown’s Wife Zopher Johnson’s Daughter?, 52 Ancestors #99

I love her name, Phoebe.  There have been other Phoebe’s in the family line – her granddaughter, Phoebe Crumley, assuredly named for her.  Nine generations later, we have another Phoebe, but her parents had never heard of Phoebe Brown or Phoebe Crumley.  I like to think that they were somehow influenced by forces unseen to select a beautiful family name.

We don’t know a lot about the original Phoebe, Jotham Brown’s wife, whose name was also spelled Phebe, Pheby and Feby.  For example, we don’t know who her parents were.

There has been speculation for years based on the close relationship of the Brown and Johnson families in Frederick County, Virginia and Greene County, Tennessee, that Phebe was the daughter of Zopher Johnson the Elder.  I wish she was, because I think Zopher is a really cool name and someone else has already done a lot of quality research on this family who was originally found near Philadelphia, but alas, I really don’t think so.  Let’s take a look at Phoebe’s life and see how the evidence stacks up.

We know that Phoebe was living in Virginia in 1768, because on the 1850 census, her daughter, Jane Brown Cooper gave Virginia as her birth location.

We also know that Zopher Johnson, “the Elder” who is Phoebe’s speculative father, was living at the Forks of the Delaware between 1754-1762 when his son, Zopher Johnson, Sr., was born.

Zopher Johnson the Elder clearly did moved to Virginia sometime before 1781 when he was on the tax list in Frederick County, VA.  This is also the first location where we find Zopher Johnson living in the same location as the Jotham Brown family.  They are both found on the 1782 tax list.  We also know that Jotham Brown was living in Hampshire County in 1778 and remained there until about 1781.

Spring Gap Road with Mountain

This land in Hampshire County is still very remote and wooded today as shown by Google Maps street view.  I can’t even imagine how forbidding it looked 237 years ago when the first settlers were obtaining land grants.

Spring Gap Hamphire Co

Spring Gap and Bethel Road where they cross the creek feeding into Little Cacapon River.  This is the area where Jotham and Phebe Brown owned land.

In 1778, Jotham Brown would have been about 38 years old and Phebe maybe a few years younger.  Given that their oldest daughter, Jane was born in 1768, and is believed to be their oldest child, Jotham would have married Phoebe about 1767 – wherever they were both living at the time.

The problem is that we don’t know where they living in 1767, although it’s likely they were living in Virginia given that their daughter was born there a year later in 1768.

Had Zopher Johnson moved to someplace in Virginia by 1767?  If so, where?  He wasn’t in Hampshire County, or at least not that we can tell, although most Hampshire County records are missing, but Virginia land grants for this area exist.

Conversely, was Jotham Brown living in Pennsylvania in 1767?  There are no records that indicate that’s the case – but it’s possible.

However, in order for Zopher Johnson’s daughter to marry Jotham Brown, they had to be in the same location at the same time.  You can’t court someone who doesn’t live nearby – at least not then in Virginia.

By 1781/1782, both families had moved to Frederick County, VA.  Was this move coordinated or pre-planned or simply circumstantial?

The 1782 tax records tell us that Jotham Brown and Phoebe had 8 children, which could mean that they had been married roughly as long as 16 years, about 1766, which correlates with their the known birth year of Jane in 1768.

There was a strong Quaker presence in Frederick County, and the Crumley and Babb families into which the Brown family would eventually marry years later in Greene County, TN were both Quaker.  Did the Johnsons and Browns live near the Quaker families on Apple Pie Ridge in Frederick County?

Catherine Crumley 2

It’s likely, because, in 1787, when Berkeley County, West Virginia was formed from Frederick County, the Johnson family is found in Berkeley County.  So was William Crumley whose land actually spanned the states, with the state line running between the fence and the white barn below.  This area was heavily Quaker, but not to the exclusion of others.

William Crumley 6

By 1790, Zopher Johnson had moved to Greene County, Tennessee.  Within a few years, William Crumley’s son, William (the second), moved to Greene County, TN as well.

However, Jotham and Phoebe Brown didn’t stay long in Frederick County.  They were gone by 1783 when they bought land in Botetourt County, VA on Brush Creek (below) where they lived until Jotham died sometime between March of 1797 when he and Phoebe sold a plot of land and May of 1800 when Phoebe and Jotham’s eleven children sold Jotham’s remaining land in preparation to move to Greene County, TN.  It’s that deed that tells us the names of all of Jotham and Phoebe’s children, at least the ones that were living at that time.  We don’t know of any that died, but in that time and place, for all of your children to live to adulthood would be remarkable indeed.

brush creek mountain from the creek

By this time, Phoebe’s oldest daughter, Jane, had married Cristopher Cooper and Phoebe probably accompanied them when they moved to Greene County.  Of Phoebe’s eleven children, nine moved to Greene County and two moved to Kentucky – so certainly Phoebe went someplace and didn’t stay in Montgomery County alone with no family and no land. That would have been a death sentence.

By this time, the Johnson and Crumley families were already established in Greene County.  The Brown/Cooper family would join the Johnson and Crumley families in the Cross Anchor area.  Was this planned, and if so, as a function of knowing each other in Frederick County…or was there something more – an already established family tie other than Sylvanus having married Ruth Johnson in Montgomery County?  If Phoebe was Zopher’s “the Elder’s” daughter, Ruth would have been Sylvanus’s first cousin.  This marriage does tell us that at least some of the Johnson family was also in Montgomery County.

William Crumley (the second) had helped to establish the Methodist Church in Greene County, TN in 1797.  At least two of Phoebe Brown’s sons-in-law were Presbyterian as both Christopher Cooper and William Stapleton signed a religious petition in Botetourt County in 1785 to establish a Reformed Church of Scotland.  Zopher the Elder’s son, Zopher Sr., is buried in the Kidwell Cemetery in Greene County which was a Methodist churchyard at one time, and some of his descendants married Presbyterian, so although we don’t know Zopher the Elder’s religious conviction, it doesn’t appear to be Quaker.  He’s not mentioned in any Quaker church records.

We know that Phoebe Brown was still alive in 1802 when she witnessed the sale of land by her daughter Jane and Jane’s husband, Christopher Cooper in Montgomery County.  By December 1803, Jane and Christopher were purchasing land in Greene County, and it’s very likely that Phoebe was along with them with her two youngest, unmarried children.  By this time, Phoebe would have been less than 60 years old, probably about 55 given that she had her last child about 1790 – not elderly by any means.

Cooper land

We don’t know when Phoebe passed away, but assuming she did not die before leaving Montgomery County, she would be buried on her daughter, Jane’s land in what is now referred to as the “Old Cooper Cemetery.”  Cousin Stevie Hughes set a beautiful marker so that it will never be lost in the underbrush again.

There are other tidbits and hints too – but those tidbits don’t speak favorably about Phoebe being the daughter of Zopher Johnson.

Phoebe and Jotham Brown had the following children, according to Stevie Hughes excellent research:

  • Jane Brown, born 1768 in Virginia, married Christopher Cooper on October 20, 1786 in Montgomery County, VA.  She died between 1856 and 1859 and is buried in the Old Cooper cemetery on Spider Stines Road near the Cross Anchor area in Greene County, on the land where she had lived.

Old Cooper burial stone

  • Sylvanus Brown born about 1771, married Ruth Johnson, daughter of Moses Johnson, a son of Zopher Johnson, in 1794 in Montgomery County, VA.  They moved to Greene County in 1805 and lived on Smith’s Fork, also known as Tillman’s Fork, very close to William Crumley who also lived on Tillman’s Fork.  In fact, four of Sylvanus’ children married Crumleys.
  • David Brown born about 1773 married Nancy Craig in 1795 in Montgomery County.  He also came to Greene County where he served in the War of 1812.
  • John Brown was born about 1774, married Elizabeth Wilson and migrated from Montgomery County to Lincoln County, KY by 1802.
  • Esther Brown born about 1775 married John Willis in 1793 in Montgomery County.  She was in Montgomery County in 1818 when John died and in 1820, but is believed to have migrated to Greene County by 1830.
  • Elizabeth Brown born about 1780 married Joshua Wilson in Lincoln County, KY in 1802.  She apparently moved there with her brother John.
  • Mary Brown born about 1780 married William Stapleton in Montgomery County.  They were in Greene County by 1812.  The family migrated to Hawkins Co., TN and then to Lee Co., VA where Mary died in 1843 and is buried in the Roberts Cemetery.  They lived close to sister Lydia Brown and her husband, William Crumley the Third.

Mary Brown Stapleton gravestone

  • Jotham Brown Jr. was born October 2, 1783 and moved with his family to Greene County where he married Margaret “Peggy” Maloney and served in the War of 1812.  He lived on the Waters of Lick Creek.  There is a Malone Cemetery on the land by the Union Church where this couple may be buried.  This is very near Tilman’s Fork.

malone cemetery

  • Mercy Brown born about 1784 married William Babb in 1807 in Greene County,  eventually settling in Hawkins County, TN..  The Babb family had lived near the Crumley family in Frederick County, VA and were Quakers.
  • William Brown was born about 1789 and married Martha Blair in 1811.  He likely died young with only one son and possibly a daughter.
  • Lydia Brown, the youngest child, was born about 1790 in Montgomery County, VA.  She would have come with her mother and siblings to Greene County where she married William Crumley (the third) on October 1, 1807.  There has been a lot of discussion, along with mitochondrial DNA testing, to determine whether or not Lydia died before 1817 or whether she lived beyond 1817.  While this may seem trivial, it isn’t, at least not to me, because my ancestor, Phebe Crumley was born to William Crumley (the third) and his wife in March of 1818.  One William Crumley married in October of 1817 to one Elizabeth Johnson.  William Crumley the Second and his son, William Crumley the Third were both living in Greene County at that time.  Suffice it to say that based on mitochondrial DNA testing, it appears that William Crumley (the third) was not the William Crumley who married Elizabeth Johnson in 1817.

With all of these children, if one’s father was named Zopher, wouldn’t you think one child, or one grandchild would be named Zopher?  Well, there isn’t, except for a son of Sylvanus but then Sylvanus married a granddaughter of Zopher Johnson….so that isn’t unexpected.  No other children or grandchildren named anyone Zopher.

To me, this is fairly damning evidence – but it isn’t everything.

Several weeks ago, I set a “honey pot” trap on Ancestry.com.  That means I intentionally added Zopher Johnson to my family tree.

Ancestry Zopher Johnson

This would cause anyone who matched my DNA and also had Zopher Johnson in their tree to pop up as a shakey leaf DNA match.  Now, clearly, some of these could be valid in that Sylvanus Brown married a Johnson granddaughter of Zopher – so I could legitimately match those descendants who legitimately have Zopher in their tree.  I could certainly match them through the Brown side.

Needless to say, I have to be exceedingly careful when I evaluate those matches – well, I would be – if I had any matches to descendants of Zopher Johnson.  At Family Tree DNA, I don’t have any Zopher matches either except for people from my own line who have Zopher in their tree as Phoebe’s father.  What I’m looking for are matches to people with whom I share no other common ancestor.

We recently found a man who descends from Zopher Johnson’s son and whose family did not intermarry with any Crumley lines.  He graciously tested, and there are no matches between any of the known Crumley descendants of Phoebe and Jotham Brown.  I have several cousins who have tested.

There are no matches to our Zopher Johnson descendant by any of the people who descend from Phoebe, Jotham Brown’s wife.

There are two things to consider – the distance of the proposed relationship and that you can’t prove a negative.

This relationship is quite a ways back in the tree, if it exists, but still, you could expect the Johnson descendant match to someone since there are several cousins who have tested.  For example, Zopher is 7 generations back from me, counting my father as generation 1, but others descended from this line are as much as 2 generations closer.

Unfortunately, we can prove, genetically that Phoebe DOES descend from Zopher Johnson (if she does), but we can never prove that she DOES NOT descend from Zopher Johnson – at least not this far removed generationally.  You simply can’t prove a negative in this case.  At this point in time, there are no matches and it doesn’t look like Phoebe is Zopher Johnson’s daughter.

We do have the mitochondrial DNA information for Phoebe, Jotham Brown’s wife, but since Zopher Johnson the Elder’s wife is unknown and there are no known daughters, there is no one available who descends from Zopher Johnson’s wife to test.

In time, there could be DNA matches that contradict the combined genealogical, location and autosomal DNA match implications so far.  But I have a feeling that’s going to be a very, very long wait.  I really don’t think that Phoebe, the wife of Jotham Brown, is the daughter of Zopher Johnson the Elder, although I’d certainly welcome any unknown information of any kind proving or even hinting otherwise.

Regardless of who her parents were, Phoebe moved a lot for a pioneer woman.  She married about 1767 and had a child someplace in Virginia in 1768.  She was first found in Hampshire County in 1778 with husband Jotham Brown.  Just a few years later, in 1781, and with a family, they moved across several mountains to Frederick County, VA where they only stayed for a year or two.  Then, in 1783, probably quite pregnant, Phoebe headed off to Botetourt County, the part that would become Montgomery County, VA where they lived on Brush Creek.  She continued to have children until about 1790 when Phoebe would have been in her early/mid-40s.  You would think by then the family would have been quite established, especially since her older children were marrying and settling nearby in Montgomery County, but that wasn’t the case.  After Jotham’s death sometime between 1797 and 1800, the entire family picked up once again and moved on, most of them to Greene County, TN.

In just under 25 years, from 1778 to 1802, Phoebe lived in 4 different frontier locations, all of which required exceedingly difficult work to clear the lands and build cabins to make them inhabitable.  This would be hard enough without children, but she had at least 11, in anything but optimal conditions, plus any she buried along the wagon trail or on those lands where they lived.  Those children, she would have had to leave behind when they moved on.

Phoebe was no shrinking violet, no wall-flower and clearly wasn’t afraid of hard work. I can’t help but wonder if she delivered her own babies, then rested for a bit before going to chop firewood to fix supper.

From today’s perspective of a nice heated house with no drafts, watching the snow fall, I can’t even imagine the privations of Phoebe’s life.  Yet, she not only survived, she thrived as did her children…who had children…who had children…who eventually had me.  I think when I want to complain about something, I’ll stop and think about Phoebe first.  After all, there is a part of her in me…and I’m doubting seriously if Phoebe, the woman who survived unknown challenges and created a family life on four frontiers, was very tolerant of whiners and complainers.

Update:  I’ve never had to update an article before I hit the publish button, but there’s a first time for everything.  I’m adding this tidbit with the hope that it will ring a bell with someone and produce actual verifiable information.

Recently, the Jotham Brown line had a Y match to a Sylvanus Brown/Esther Dayton family from Long Island, NY who was found there in the early 1700s.  Sylvanus is such an unusual name that along with the Y DNA match, it’s quite compelling.  We know they do share a common Brown ancestor, we just don’t know where or when.

In addition, another long-time researcher tells me that the Cooper family was already established in Montgomery County when Jotham Brown and Phebe moved there in 1783.  Jotham and Phebe’s daughter, Jane, married Christopher Cooper, son of James, whose will was contested, and whose brother was named…Sylvanus.  So we have two families that include the very unusual name of Sylvanus meeting (again?) in Montgomery County, VA.

According to “Annals of Southwest Virginia”, Christopher and John Cooper were the first to acquire land on Brush Creek of Little River (Feb. and Nov., 1782). Jotham acquired land there August 20, 1783.  Moses Johnson acquired 200 acres on Brush Creek August 20, 1783, the same day Jotham Brown acquired his land.  James King (another Long Island and New Jersey surname) acquired 300 acres on Brush Creek September 2, 1782, so he was there early with Christopher Cooper.

Furthermore, the Zopher Johnson line that went to Illinois carries a story that Zopher Johnson Jr. (the grandson of Zopher Johnson the Elder) had an inheritance on Long Island but never pursued it due to lack of money.  True?  We don’t know, but that’s a very odd location for oral history out of Illinois.

Is this coincidence?  We don’t know, but if anyone has any information about the Johnson, Brown or Cooper families that can unite them on Long Island (or elsewhere) or provide an explanation for what is today, circumstantial evidence, I would be exceedingly grateful.