Category Archives: Family Tree DNA

Y-DNA Haplogroup O – When and How Did It Get to the Americas?

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Y-DNA Haplogroup O has been found in male testers descended from a Native American ancestor, or in Native American tribes in the Americas – but sometimes things are more complex than they seem. The story of when and how haplogroup O arrived in the Americas is fascinating – and not at all what you might think.

Introduction

The concept of Native American heritage and indigenous people can be confusing. For example, European Y-DNA haplogroup R is found among some Native American men. Those men may be tribal members based on their mother’s line, or their haplogroup R European Y-DNA may have been introduced either through adoption practices or traders after the arrival of Europeans.

There is unquestionable genetic evidence that the origin of Haplogroup R in the Americas was through colonization, with no evidence of pre-contact indigenous origins.

Y-DNA testing and matching, specifically the Big Y-700 test, with its ability to date the formation of haplogroups very granularly, has successfully identified the genesis of Y-DNA haplogroups and their movement through time.

We’ve spent years trying to unravel several instances of Native American Y-DNA Haplogroup O and their origins. Native American, in this context, means that men with haplogroup O are confirmed to be Native American at some point in documented records. This could include early records, such as court or probate records, or present-day members of tribes. There is no question that these men are recognized as Native American in post-contact records or are tribal members, or their descendants.

What has not been clear is how and when haplogroup O entered the Native American population of these various lineages, groups, or tribes. In other words, are they indigenous? Were they here from the earliest times, before the arrival of colonists, similar to Y-DNA haplogroups C and Q?

This topic has been of great interest for several years, and we have been waiting for additional information to elucidate the matter, which could manifest in several ways:

  1. Ancient pre-contact DNA samples of haplogroup O in the Americas, but none have been found.
  2. Current haplogroup O testers in Native American peoples across the North and South American continents, forming a connecting trail genetically, geographically, and linearly through time. This has not occurred.
  3. Big-Y DNA matches within the Americas between Haplogroup O Native American lines unrelated in a genealogical timeframe whose haplogroup formation pre-dates European contact. This has not occurred.
  4. Big-Y DNA matches between Haplogroup O men whose haplogroups were formed in the Americas after the Beringian migration and expansion that scientists agree occurred at least 12-16K years ago, and possibly began earlier. Earlier human lineages, if they existed, may not have survived. A later Inuit and Na-Dené speaker circumpolar migration occurred 4-7K years ago. This has not occurred.
  5. Big-Y DNA matches with men whose most recent common ancestor haplogroup formation dates connect them with continental populations in other locations, outside of North and South America. This would preclude their presence in the Americas after the migrations that populated the Americas. This has occurred.

The Beringian migration took place across a now-submerged land bridge connecting the Chutkin Peninsula in Russia across the Bering Strait with the Seward Peninsula in Alaska.

By Erika Tamm et al – Tamm E, Kivisild T, Reidla M, Metspalu M, Smith DG, et al. (2007) Beringian Standstill and Spread of Native American Founders. PLoS ONE 2(9): e829. doi:10.1371/journal.pone.0000829. Also available from PubMed Central., CC BY 2.5, https://commons.wikimedia.org/w/index.php?curid=16975303

Haplogroup O is clearly Native American in some instances, meaning that it occurs in men who are members of or descend from specific Native American tribes or peoples. One man, James Revels, is confirmed in court records as early as 1656. However, ancestors of James Revels fall into category #5, as their upstream parental haplogroup is found in the Pacific islands outside the Americas after the migration period.

Based on available evidence, the introduction of haplogroup O appears to be post-contact. Therefore, haplogroup O is not indigenous to the Americans in the same sense as haplogroups Q and C that are found widespread throughout the Americas in current testers who are tribal members, descendants of tribal members, and pre-contact ancient DNA as mapped in the book, DNA for Native American Genealogy.

Ancient DNA

Haplogroup C is found in both North and South America today, as are these ancient DNA locations.

Haplogroup Q is more prevalent than Haplogroup C, and ancient DNA remains are found throughout North and South America before colonization.

No ancient DNA for Haplogroup O has been discovered in the Americas. We do find contemporary haplogroup O testers in regional clusters, which we will analyze individually.

Let’s take a look at what we have learned recently.

Wesley Revels’ Lineage

Wesley Revels was the initial Y-DNA tester whose results identified Haplogroup O as Native American, proven by a court record. That documentation was critical, and we are very grateful to Wesley for sharing both his information and results.

Wesley’s ancestor, James Revels, was Native American, born about 1656 and bound to European planter, Edward Revell. James was proven in court to be an Accomack “Indian boy” from “Matomkin,” age 11 in 1667. James was bound, not enslaved, until age 24, at which time he was to be freed and receive corn and clothes.

James had died by 1681 when he was named several times in the Accomack County records as both “James, an Indian” and “James Revell, Indian,” in reference to his estate. James lived near Edward Revell, his greatest creditor and, therefore, administrator of his estate, and interacted with other Indian people near Great Matompkin Neck. Marie Rundquist did an excellent job of documenting that here. Additional information about the Revels family and Matomkin region can be found here.

The location where Edward Revell lived, Manokin Hundred, was on the water directly adjacent the Great Matomkin (now Folly Creek) and Little Matomkin Creeks, inside the Metomkin Inlet. The very early date tells us that James Revels’s paternal ancestor was in the colonies by 1656 and probably born about 1636, or perhaps earlier.

Lewis and Revels men are later associated with the Lumbee Tribe, now found in Robeson and neighboring counties in North Carolina. The Lewis line descends from the Revels lineage, as documented by Marie and Wesley. Other men from this line have tested and match on lower-level STR markers, but have not taken the much more granular and informative Big-Y test.

Until recently, the men who matched Wesley Revels closely on the Big-Y test were connected with the Revels line and/or the Lumbee.

Wesley has a 37-marker STR match to a man with a different surname who had not tested beyond that level, in addition to several 12-marker STR matches to men from various locations. Men who provided known ancestral or current locations include one from Bahrain, two from the Philippines, and three from China. Those men have not taken the Big-Y, and their haplogroups are all predicted from STR results to O-M175 which was formed in Asia about 31,000 years ago.

12-marker matches can reach thousands of years back in time. Unless the matches share ancestors and match at higher levels, 12-marker matches are only useful for geographic history, if that. The Big Y-700 test refines haplogroup results and ages from 10s of thousands of years to (generally) within a genealogically relevant timeframe, often within a couple hundred years.

One of Wesley’s STR matches, Mr. Luo, has taken a Big Y-700 test. Mr. Luo descends directly from Indonesia in the current generation and is haplogroup O-CTS716, originating about 244 BCE, or 2244-ish years ago. Mr. Luo does not match Wesley on the Big-Y test, meaning that Wesley and Mr. Luo have 30 or more SNP differences in their Big-Y results, which equates to about 1,500 years. The common ancestor of Wesley Revels and Mr. Luo existed more than 1,500 years ago in Indonesia. It’s evident that Mr. Luo is not Native American, but his location is relevant in a broader analysis.

There is no question that Wesley’s ancestor, James Revels, was Native American based on the court evidence. There is also no question that the Revels’ paternal lineage was not in the Americas with the Native American migration group 12-16K years ago.

The remaining question is how and when James Revels’ haplogroup O ancestor came to be found on the Atlantic seaboard in the early/mid 1600s, only a few years after the founding of Jamestown.

The results of other Haplogroup O men may help answer this question.

Mr. Lynn

Another haplogroup O man, Mr. Lynn, matches Wesley on STR markers, but not on the Big-Y test.

Mr. Lynn identified his Y-DNA line as Native American, although he did not post detailed genealogy. More specifically, we don’t know if Mr. Lynn identified that he was Native on his paternal line because he matches Wesley, or if the Native history information was passed down within his family, or from genealogical research. Mr. Lynn could also have meant generally that he was Native, or that he was Native “on Dad’s side,” not specifically his direct patrilineal Y-line.

Based on Mr. Lynn’s stated Earliest Known Ancestor (EKA) and additional genealogical research performed, his ancestor was John Wesley Lynn (born approximately 1861, died 1945), whose father was Victor Lynn. John’s death certificate, census, and his family photos on Ancestry indicate that he was African American. According to his death certificate, his father, Victor Lynn, was born in Chatham Co., NC, just west of Durham.

Family members are found in Baldwin Township, shown above.

I did not locate the family in either the 1860 or 1870 census. In 1860, the only Lynn/Linn family in Chatham County was 50-year-old Mary Linn and 17-year-old Jane, living with her, presumably a daughter. Both are listed as “mulatto” (historical term) with the occupation of “domestic.” They may or may not be related to John Wesley Lynn.

In 1870, the only Linn/Lynn in Chatham County is John, black, age 12 or 13 (so born in 1857 or 1858), farm labor, living with a white family. This is probably not John Wesley Lynn given that he is found with his mother in 1880 and the ages don’t match.

In 1880. I find Mary Lynn in Chatham County, age 48, single, black, with daughter Eliza Anne, 20, mulatto, sons John Wesley, 14 so born about 1866, and Charles 12, both black. Additionally, she is living with her nieces and nephews, Cephus, black, 12, Lizzie, 7, mulatto, Malcom, 4, mulatto, William H, 3, mulatto (I think, written over,) and John age 4, mulatto. The children aged 12 and above are farm labor.

In 1880, I also find Jack Lynn, age 28, black, married with 3 children, living beside William Lynn, 25, also married, but with no children.

Trying to find the family in 1870 by using first name searches only, I find no black Mary in 1870 or a mulatto Mary with a child named Jack or any person named Cephus by any surname. I don’t find Jack or any Lynn/Linn family in Chatham County.

The 1890 census does not exist.

In the 1900 census, I find Wesley Lynn in Chatham County, born in January of 1863, age 37, single, a boarder working on the farm of John Harris who lives beside Jack Lynn, age 43, born in April of 1857. Both Lynn men are black. I would assume some connection, given their ages, possibly or probably brothers.

In 1940, John Wesley Lynn, age 74, negro (historical term), is living beside Victor Lynn, age 37, most likely his son.

I could not find Victor Lynn, John Wesley Lynn’s father in any census, so he was likely deceased before 1880 but after 1867, given that Mary’s son Charles Lynn was born in 1868, assuming Mary’s children had the same father. The fact that Mary was listed as single, not married nor widowed suggests enslavement, given that enslaved people were prohibited from legally marrying.

About the only other assumption we can make about Victor Sr. is that he was probably born about 1832 or earlier, probably in Chatham County, NC based on John Wesley’s death certificate, and he was likely enslaved.

Subclades of Haplogroup O

Both the Revels and Lynn men are subclades of haplogroup O and both claim Native heritage – Wesley based on the Revels genealogy and court documents, and Mr. Lynn based on the Native category he selected to represent his earliest known paternal ancestor at FamilyTreeDNA.

Both men have joined various projects, including the American Indian Project, which provides Marie and me, along with our other project co-administrators, the ability to work with and view both of their results at the level they have selected.

How Closely Related Are These Haplogroup O Men?

How closely related are these two men?

By Viajes_de_colon.svg: Phirosiberiaderivative work: Phirosiberia (talk) – Viajes_de_colon.svg, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=8849049

  • Do the haplogroups of the Revels men and Mr. Lynn converge in a common ancestor in a timeframe BEFORE colonialization, meaning before Columbus “discovered” the Caribbean islands when colonization and the slave trade both began?
  • Do the haplogroups converge on North or South American soil or elsewhere?
  • Is there anything in the haplogroup and Time Tree information that precludes haplogroup O from being Native prior to the era of colonization?
  • Is there anything that confirms that a haplogroup O male or males were among the groups of indigenous people that settled the Americas sometime between 12 and 26 thousand years ago? Or even a later panArctic or circumpolar migration wave?

Haplogroup O is well known in East Asia, Indonesia, and the South Pacific.

Another potential source of haplogroup O is via Madagascar and the slave trade.

The Malagasy Roots Project has several haplogroup O individuals, including the Lynn and Revels men, who may have joined to see if they have matches. We don’t know why the various haplogroup O men in the project joined. Other haplogroup O men in the project may or may not have proven Malagasay heritage.

Information provided by the project administrators is as follows:

The people of Madagascar have a fascinating history embedded in their DNA. 17 known slave ships came from Madagascar to North America during the Transatlantic Slave Trade. As a result, we find Malagasy DNA in the African American descendants of enslaved people, often of Southeast Asian origin. One of the goals of this project is to discover the Malagasy roots of African Americans and connect them with their cousins from Madagascar. Please join us in this fascinating endeavor. mtDNA Haplogroups of interest include: B4a1a1b – the “Malagasy Motif”, M23, M7c3c, F3b1, R9 and others Y-DNA Haplogroups include: O1a2 – M50, O2a1 – M95/M88, O3a2c – P164 and others

Resources:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987306/  http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1199379/  http://mbe.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=19535740  http://www.biomedcentral.com/1471-2156/15/77  http://www.biomedcentral.com/1471-2164/10/605

The Malagasy group only has one other man who is haplogroup O and took the Big-Y test, producing haplogroup O-FTC77008. Of course, we don’t know if he has confirmed Madagascar ancestry, and his haplogroup is quite distant from both Revels and Lynn in terms of when his haplogroup was formed.

Viewing the Malagasy Project’s Group Time Tree, above, the common ancestor between those three men lived about 28K BCE, or 30,000 years ago.

Haplogroup O Project Group Time Tree

The Haplogroup O Project Time Tree provides a better representation of haplogroup O in general given that it has a much wider range of samples.

On this tree, I’ve labeled the haplogroup formation dates, along with the Revels/Lewis line which descends from O-FT45548. This haplogroup includes one additional group member whose surname is locked, as he hasn’t given publication permission. The haplogroup formation date of 1766 occurs approximately 85 years after James Revel’s birth, so is attributable to some, but not all of his descendants. At least one descendant falls into the older Haplogroup O-BY60500.

The common ancestor of all three, meaning Revels, Lewis, and the man whose name is locked and does not know his genealogy, is haplogroup O-BY60500, born about 1741.

Their ancestral haplogroup before that, O-FT11768, is much older.

Two Filipino results are shown on and descending from the parent branch of O-FT11768, formed about 3183 BCE, or about 5183 years ago. This tells us that the ancestors of all these men were in the same place, most likely the Philippines, at that time.

3183 BCE (5180 years ago) is well after the Native American migration into the Americas.

Discover Time Tree

Obviously, not every tester joins a project, so now I’m switching to the Discover Time Tree which includes all Y-DNA haplogroup branches. Their common haplogroup, O-FT11768, has many branches, not all of which are shown below. I’m summarized unseen branch locations at bottom left.

Expanding the Time Tree further to view all of the descendant haplogroups of O-FT11768, we see that this was a major branch with many South Pacific results, including the branch of O-FT22410, bracketed in red, which has three members.

One is Mr. Lynn whose feather indicates Native American as his EKA country selection, one is a man whose ancestor is from Singapore, and one is an unknown individual who did not enter his ancestor’s country of origin.

Geography

Wesley’s STR match list, which can reflect matches further back in time than the Big-Y test, includes islands near Singapore. This geography aligns with what is known about haplogroup O.

The distance between this Asian region and continental America, 9000+ miles distant by air, is remarkable and clearly only navigable at that time by ship, meaning ships with experienced crew, able to navigate long distances with supplies and water.

We know that in 760 CE, about 1240 years ago, Mr. Lynn’s haplogroup O-F24410 was formed someplace in the South Pacific – probably in Malaysia or a nearby island. This region, including the Philippines, is home to many haplogroup O men. The majority of haplogroup O is found in Asia, the South Pacific, and Diaspora regions.

We know that Hawaii was populated by Polynesian people about 1600 years ago, prior to the age of colonization. Hawaii is almost 7000 miles from Singapore.

Here’s the challenge. How did these haplogroup O men get from the South Pacific to Virginia? Mr. Lynn and the Singapore tester share a common ancestor about 1240 years ago, or 760 CE.

There is no known or theorized Native American settlement wave across Beringia as late as 760 CE. We know that the parent haplogroup was someplace near Singapore in approximately 760 CE.

Two Filipino men and the Revels’ ancestors were in the same location in the Pacific Islands 5180 years ago. How did they arrive on the Eastern Shore in Virginia, found in the Native population, either in or before 1656 when James Revels was born?

What happened in the 3500 years between those dates that might explain how James Revel’s ancestor made that journey?

Academic Papers

In recent years, there has been discussion of possible shoreline migration routes along the Russian coast, Island hopping along Alaska, Canada, and what is now the US, known as the Kelp Highway or Coastal Migration Route – but that has yet to be proven.

Even if that is the case, and it’s certainly a possibility, how did this particular group of men get from the Pacific across the continent to the Atlantic shore in such a short time, leaving no telltale signs along the way? The Coastal Migration Theory hypothesis states that this migration occurred from 12-16 thousand years ago, and then expanded inland over the next 3-5K years. They could not have expanded eastward until the glaciers receded. Regardless, the parent haplogroup and associated ancestors are still found in the Philippines and South Pacific 5000 years ago – after that migration and expansion had already occurred.

The conclusion of the paper is that there is no strong evidence for a Pacific shoreline migration. Regardless, that’s still thousands of years before the time range we’re observing.

We know that the Lynn ancestor was with men from Indonesia in 760 CE, and the Revels ancestor was with men from the Pacific Islands, probably the Philippines, 5180 years ago. They couldn’t have been in two places at the same time, so the ancestors of Revels and Lynn were not in the Americas then.

A 2020 paper shows that remains from Easter Island (Rapa Nui) show Native American DNA, and suggests that initial contact occurred between the two cultures about 1200 CE, or about 800 years ago, but there is not yet any pre-contact or post-contact ancient Y-DNA found in the Americas that shows Polynesian DNA. Furthermore, the hypothesis is that the DNA found on Easter Island came from the Americas, not vice versa. The jury is still out, but this does show that trans-Pacific contact between the two cultures was taking place 800 years ago, at least two hundred years pre-European contact.

Australasian migration to South America is also suggested by one set of remains found in Brazil dating from more than 9000 years ago, but there have been no other remains found indicating this heritage, either in Brazil, or elsewhere in the Americas.

Based on the Time Tree dates of the Haplogroup O testers in our samples, we know they were in the Islands of Southeast Asia after this time period. Additionally, there are no Australia/New Zealand matches.

The Spanish

The Spanish established an early trade route between Manila and Acapulco beginning in 1565. Consequently, east Asian men left their genetic signature in Mexico, as described in this paper.

Historians estimate that 40-129K immigrants arrived from Manilla to colonial Mexico between 1565 and 1815, with most being enslaved upon arrival. Approximately one-third of the population in Manilla was already enslaved. Unfortunately, this paper focused only on autosomal genome-wide results and did not include either Y-DNA, nor mitochondrial. However, the paper quantifies the high degree of trade, and indicates that the Philippines and other Asian population haplotypes are still prevalent in the Mexican population.

In 2016, Dr. Miguel Vilar, the lead scientist with the National Geographic Genographic project lectured in Guam about the surprising Native American DNA found in the Guam population and nearby islands. He kindly provided this link to an article about the event.

Guam was colonized by Spain. In the image from the Boxer Codex, above, the local Chamorro people greet the Manila Galleon in the Ladrones Islands, as the Marianas were called by the Spanish, about 1590.

Native Hawaiians descend from Polynesian ancestors who arrived in the islands about 400 CE, or about 1600 years ago. Captain Cook, began the age of European contact in Hawaii in 1778.

Five Possibilities

There are five possible origins of haplogroup O in the Americas.

  • Traditional migration across Beringia with the known migrations, estimated to have occurred about 12-16K years ago.
  • A Kelp Highway Coastal Migration which may have occurred about 12-16K years ago and dispersed over the next 3-5K years.
  • Circumpolar migration – specifically Inuit and Na-Dene speakers, about 4-6K years ago.
  • Post-contact incorporation from the Pacific Islands resulting from shipping trade on colonial era ships sometime after 1565.
  • Post-contact incorporation from Madagascar resulting from the importation of humans who may or may not have been enslaved upon arrival.

Do we have any additional evidence?

Other Haplogroup O DNA

From my book, DNA for Native American Genealogy:

Testers in haplogroup O-BY60500 and subclade O-FT45548 have proven Native American heritage.

We have multiple confirmed men from a common ancestor who is proven to be an enslaved Accomack “Indian boy,” James Revell, born in 1656, “belonging to the Motomkin” village, according to the Accomack County, Virginia court records. These men tested as members of haplogroup O-F3288 initially, after taking the Big Y-500 test. However, upgrading to the Big Y-700 produced more granular results and branches reflecting mutations that occurred since their progenitor was born in 1656.

Unfortunately, other than known descendants, these men have few close Y-DNA or Big Y-700 matches.

Without additional men testing from different unrelated lines, or ancient haplogroup O being discovered, we cannot confirm that this haplogroup O male’s ancestor was not introduced into the Matomkin Tribe in some way post-contact. Today, one descendant from this line is a member of the Lumbee Tribe.

However, that isn’t the end of the haplogroup O story.

The Genographic Project data shows one Haplogroup O Tlingit tribal member from Taku, Alaska, along with several testers from Mexico that indicate their paternal line is indigenous. Some people from Texas identify their paternal line as Hispanic.

Another individual indicates they were born on the Fountain Indian Reserve, in British Columbia and speaks the St’at’imcets language, an interior branch of Coastal Salish.

Haplogroup O has been identified as Native American in other locations as well.

Much of the information about Haplogroup O testers was courtesy of the Genographic Project, meaning we can’t contact those people to request upgraded tests, and we can’t obtain additional information in addition to what they provided when they tested. As an affiliate researcher, I’m very grateful to the National Geographic Society’s Genographic project for providing collaborative data.

When the book was published, the Discover Time Tree had not yet been released. We have additional information available today, including the dates of haplogroup formation.

FamilyTreeDNA Haplotree and Discover

The FamilyTreeDNA Haplotree (not to be confused with the Discover Time Tree) shows 10 people at the O-M175 level in Mexico, 10 people in the US report Native American heritage, 2 in Jamaica, and one each in Peru, Panama, and Cuba. There’s also one tester from Madagascar.

Altogether, this gives us about 35 haplogroup O males in the Americas, several with Native heritage.

Please note that I’ve omitted Hawaii in this analysis and included only North and South America. The one individual selecting Native Hawaiian (Kanaka Maoli) is in haplogroup O-M133.

Let’s look at our three distinct clusters.

Cluster 1 – Pacific Northwest – Alaska and Canada

We have a cluster of three individuals along the Pacific Coast in Alaska and Canada who have self-identified as Native, provided a tribal affiliation, and, in some cases, the spoken language.

How might haplogroup O have arrived in or near Vancouver, Washington? We know that James Cook “discovered” Hawaii in 1778, naming it the Sandwich Islands. By 1787, a female Hawaiian died en route to the Pacific Northwest, and the following year, a male arrived. Hawaii had become a provisioning stop, and the Spanish took Hawaiians onto ships as replacement workers.

Hawaiian seamen, whalers, and laborers began intermarrying with the Native people along the West Coast as early as 1811. Their presence expanded from Oregon to Alaska. Migration and intermarriage along the Pacific coast began slowly, but turned into a steady stream 30 years later when we have confirmed recruitment and migration of Hawaiian people

In 1839, John Sutter recruited a small group of 10 Hawaiians to travel with him to the then-Mexican colony of Alta, California.

By the mid-1800s, hundreds of Hawaiians lived in Canada and California. In 1847, it was reported that 10% of San Francisco’s residents were Hawaiian. Some of those people integrated with the Native American people, particularly the Miwok and Maidu. The village of Verona, California was tri-lingual: Hawaiian, a Native language, and English, and is today the Sacramento-Verona Tribe.

This article provides a history of the British Company who administered Fort Vancouver, near Vancouver, Washington, that included French-Canadians, Native Americans and Hawaiians. In 1845, 119 Hawaiians were employed at the fort. One of the 119, Opunuia, had signed on as an “engagé,” meaning some type of hired hand or employee, with the Hudson Bay Company for three years, after which he would be free to return home to Honolulu or establish himself in the Oregon Country. He married a woman from the Cascade Tribe.

The descendants of the Hawaiian men and Native women were considered tribal members. In most tribes, children took the tribal status and affiliation of the mother.

The Taku and Sitka, Alaska men on the map are Tlingit, and the man from British Columbia is from the Fountain Indian Reserve.

Hawaiian recruitment is the most likely scenario by which haplogroup O arrived in the tribes of the Pacific Northwest. In that sense, haplogroup O is indeed Native American but not indigenous to that region. The origins of haplogorup O in the Pacific Northwest are likely found in Hawaii, where it is indigenous, and before that, Polynesia – not due to a Beringian crossing.

Cluster 2 – Mexico

We find a particularly interesting small cluster of 4 haplogroup O individuals in interior Mexico.

In the 1500s, Spain established a trade route between Mexico and Manilla in the Philippines.

In 1564, four ships left Mexico to cross the Pacific to claim Guam and the Philippines for King Philip II of Spain. The spice trade, back and forth between Mexico and the Philippines began the following year and continued for the next 250.

Landings occurred along the California coast and the western Mexican coastline. The majority of the galleon crews were Malaysian and Filipino who were paid less than the Spanish sailors. Slaves, including people from the Marianas were part of the lucrative cargo.

One individual in Texas reports haplogroup O and indicates their paternal ancestors were Hispanic/Native from Mexico. A haplogroup O cluster claiming Native heritage is found near Zacatecas, Fresnillo and San Luis Potosi in central Mexico. Additionally, mitochondrial haplogroup F, also Asian, is found there as well. Acapulco is the lime green pin.

An additional haplogroup O tester with Native heritage is found in Lima, Peru.

Haplogroup O men are found in Panama, Jamaica and Cuba, but do not indicate the heritage of their paternal ancestral line. None of these men have taken Big-Y tests, and some may well have arrived on the slave ships from Madagascar, especially in the Caribbean. This source attributes some enslaved people in Jamaica to Hawaiian voyages.

I strongly suspect that the Mexican/Peru grouping in close proximity to the Pacific coastline is the result of the Manilla-Mexico 250-year trade route. The Spanish also plied those waters regularly. Big Y testing of those men would help flesh-out their stories – when and how haplogroup O arrived in the local population.

Cluster 3 – East Coast

At first glance, the East Coast grouping of men with a genetic affinity to the people of the Philippines and Indonesia seems more difficult to explain, but perhaps not.

On the East Coast, we have confirmed reports of whalers near Nantucket as early as 1765 utilizing crewmen from Hawaii, known then as the Sandwich Islands, Tahiti, and the Cape Verde Islands off of Africa. A thorough review of early literature might well reveal additional information about early connections with the Sandwich Islands, and in particular, sailors, crew, or enslaved people.

The Spanish and French were the first to colonize the Philippines by the late 1500s. They had discovered the Solomon Islands, Melanesia, and other Polynesian Islands, and by the early 1600s, the Dutch were involved as well.

The Encyclopedia Britanica further reports that Vasco Balboa first sailed into the Pacific in 1513 and seven years later, Ferdinand Magellan rounded the tip of South America. The Spanish followed, establishing a galley trade between Manila, in the Philippines and Acapulco in western Mexico.

While I found nothing specific stating that the earliest voyages brought men from the Philippines and Oceania back to their European home ports with them, we know that early European captains on exploratory voyages took Native people from the east coast of the Americas on their return journey, so there’s nothing to preclude them from doing the same from the Pacific. The early explorers stayed for months among the Oceanic Native peoples. If they were short on sailors for their return voyage, Polynesian men filled the void.

We know that the Spanish took slaves as part of their trade. We know that the ships in the Pacific took sailors from the islands. If the men themselves didn’t stay in the locations they visited, it’s certainly within the realm of possibility that they fathered children with local, Native women. Furthermore, given that the slave trade was lucrative, it’s also possible that some Pacific Island slaves were taken not as crew but with the intention of being sold into bondage. Other men may have escaped the ships and hidden among the Native Tribes along the eastern seaboard.

Fishing in Newfoundland and exploration in what would become the US was occurring by 1500, so it’s certainly possible that some of the indigenous people from Indonesia and the Philippines were either stranded, sold to enslavers, escaped, or chose to join the Native people along the coastline in North America. Ships had to stop to resupply rations and take on fresh water.

We know that by the mid-1600s, James Revels, whose father carried haplogroup O, had been born on the Atlantic coast of Virginia or Maryland, probably on the Delmarva Peninsula, short for Delaware, Maryland, Virginia, where the Accomac people lived.

There are other instances of haplogroup O found along the east coast.

On the eastern portion of the haplogroup O map from the book, DNA for Native American Genealogy, we find the following locations:

  • Hillburn, NY – man identified as “Native American Black.”
  • Chichester County, PA – Genographic tester identified the location of his earliest known ancestor – included here because O is not typically found in the states.
  • Accomack County, VA – Delmarva peninsula – James Revels lineage
  • Robeson County, NC – Lewis and Revels surname associated with the Lumbee
  • Chatham County, NC – Lynn ancestor’s earliest known location
  • Greene County, NC – enslaved Blount ancestor’s EKA in 1849

The genesis of Mr. Blount’s enslaved ancestor is unclear. Fortunately, he took a Big Y-700 test.

Mr. Blount’s only Big-Y match is to a man from the United Arab Emirates (UAE), but the haplogroup history includes Thailand, which is the likely source of both his and his UAE matches’ ancestors at some point in time. Their common ancestor was in Thailand in 336 CE, almost 1700 years ago.

All surrounding branches of haplogroup O on the Time Tree have Asian testers, except for the one UAE gentleman.

The Blount Haplogroup O-FTC77008 does not connect with the common ancestral haplogroup of Lynn and Revels, so these lineages are only related someplace in Oceana prior to O-F265, or more about  30,000 years ago. Their only commonality other than their Asian origins is that they arrived on the East Coast of the Americas.

We know that the Spanish were exploring the Atlantic coastline in the 1500s and were attempting to establish colonies. In 1566, a Spanish expedition reached the Delmarva Peninsula. This spit of land was contested and changed hands several times, belonging variously to the Spanish, Dutch, and British by 1664.

Furthermore, we also know that the ships were utilizing slave labor. One of the Spanish ships wrecked in the waters off North Carolina near Hatteras or Roanoke Island before the Lost Colony was abandoned on Roanoke Island in 1587. The Croatan Indians reported that in memorable history, several men, some of whom were reported to be slaves, had survived the wreck and “disappeared” into the hinterlands – clearly running for their lives.

These men, if they survived, would have been incorporated into the Native population as there were no other settlements at the time. Variations of this scenario may have played out many times.

James Revels’ ancestor could have arrived on any ship, beginning with exploration and colonization in the early 1500s through the mid-1650s.

By the time the chief bound the Indian boy who was given the English name James to Edward Revell, James’s Oceanic paternal ancestor could have been 4, 5 or 6 generations in the past – or could have been his father.

The Accomack was a small tribe, loosely affiliated with the Powhatan Confederacy along the Eastern Shore. By 1700, their population had declined by approximately 90% due to disease. A subgroup, the Gingaskins, intermarried with African Americans living nearby. After Nat Turner’s slave rebellion of 1831, they were expelled from their homelands.

The swamps near Lumberton in Robeson County, NC, became a safe haven for many mixed-race Native, African, and European people. The swamps protected them, and they existed, more or less undisturbed, for decades. Revels and Lewis descendants are both found there.

Many Native Americans were permanently enslaved alongside African people – and within a generation or so, their descendants knew they were Native and African, but lost track of which ancestors descended from which groups. Life was extremely difficult back then. Generations were short, and enslaved people were moved from place to place and sold indiscriminately, severing their family ties entirely, including heritage stories.

Returning to the Discover Time Tree Maps

Wesley Revels has STR matches with several men from Indonesia, China, and the Philippines. It would be very helpful if those men would upgrade to the Big Y-700 so that we can more fully complete the haplogroup O branches of the Time Tree.

The common Revels/Lewis ancestor, accompanied by two descendant men on different genetic branches from the Philippines, was born about 5180 years ago. There is no evidence to suggest Haplogroup O-FT11768 was born anyplace other than in the Philippines.

How did the descendant haplogroups of O-FT45548 (Revels, Lewis, and an unnamed man) and O-F22410 (Lynn) arrive in Virginia or anyplace along the Atlantic seaboard?

Hawaii wasn’t settled until about 1600 years ago. We know Hawaiians integrated with the Pacific Coast Native tribes in the 1800s, but James Revels was in Virginia in 1656..

We know that the Spanish established a mid-1500s trade route between Manila and Acapulco, leaving their genetic signature in western Mexico.

None of these events fit the narrative for the Revels or the Lynn paternal ancestor.

Furthermore, the Revels and Lynn lines do not connect on North American soil, as both descend from the same parent haplogroup, O-FT11768, 5180 years ago in the Philippines. This location and history suggest a connection with the Spanish galleon trade era. The haplogroup formation clearly predates that trade, which means those men were still in the Philippines, not already living on the American continents. Therefore, the descendants of the haplogroup O-FT11768 arrived in Virginia and North Carolina sometime after that haplogroup formation 5100 years ago.

The Lynn ancestor connects with a man from Singapore in 760 CE, or just 1240 years ago. A descendant of haplogroup O-F22410 arrived in North Carolina sometime later.

It does not appear, at least not on the surface, that there is a connection through Madagascar, although we can’t rule that out without additional testers. If the connection is through Madagascar, then their ancestors were likely transported from Indonesia to Madagascar, then as enslaved people from Madagascar to the Atlantic colonies to be sold. However, James Revels was not enslaved. He was clearly Native and bound to a European plantation owner, who did, in fact, free him as agreed and subsequently loaned him money.

Based on the dates involved, and when we know they were in Oceania, an arrival along the west coast, followed by a quick migration across the country to a peninsula of land in the Atlantic, is probably the least likely scenario. There is also no historical or ancient haplogroup O DNA found anyplace between the west and east coasts, nor in the Inuit or Na-Dene speakers. The Navajo, who speak the Na-Dené language, migrated to the Southwest US around 1400 CE, but haplogroup O has not been found among Na-Dené speakers.

It’s a long way from Singapore and the Philippines to Madagascar, so while the coastal migration scenario is not impossible, it’s also not probable, especially given what we know about the Spanish Pacific trade that existed profitably for 250 years.

However, one haplogroup O subgroup arrived in the UAE by some methodology after 336 CE.

It’s entirely possible, indeed probable, that haplogroup O arrived in the Americas for various reasons, on different paths, in different timeframes.

Haplogroup O was found in people in the Americas after colonization had begun. There has been no ancient Haplogroup O DNA discovered, and there’s evidence indicating that these instances of haplogroup O could not have arrived in any of the known Beringia migrations nor the theorized Coastal or Kelp migration. We know the East Coast Cluster is not a result of the West Coast 19th-century migration because James Revels was in court one hundred and fifty years before the Hawaiians were living among the Native people along the Pacific coastline.

There’s nothing to indicate that the Mexican group that likely arrived beginning in the mid-1500s for the next 250 years as a result of the Indonesian trade route migrated to the east coast, or vice versa. That’s also highly unlikely.

The most likely scenario is that Mr. Lynn’s, Mr. Blount’s, and James Revels’ ancestors were brought on trade ships, either as sailors or enslaved men. They may not have stayed, simply visited. They may each have arrived in a completely different scenario, meaning Mr. Blount’s ancestors could have been enslaved arrivals from Madagascar, Mr. Lynn’s from Indonesia, and Mr. Revel’s as a crew member on a Spanish ship. We simply don’t know.

James Revels’ descendants were Native through his mother’s tribe, as confirmed in the 1667 court records. However, the Revels and Lynn lineages weren’t Native as a result of their paternal haplogroup O ancestors crossing Beringia into the Americas with Native American haplogroups Q and C. Instead, the Lynn and Revels migration story is quite different. Their ancestors arrived by ship. The journey was long, perilous, and far more unique than we could have imagined, taking them halfway around the world by water.

Timeline

There’s a lot of information to digest, so I’ve compiled a timeline incorporating both genetic and historical information for easy reference.

  • 30,000 years ago (28,000 BCE) – haplogroup O-F265, common Asian ancestor  of Mr. Blount, the Revels/Lewis group, Mr. Lynn, and an unknown Big-Y tester in the Malagasy group project
  • 12,000-16,000 years ago – Indigenous Americans arrived across now-submerged Beringia
  • 12,000-16,000 years ago – possible Coastal Migration route may have facilitated a secondary source of indigenous arrival along the Pacific coastline of the Americas
  • 4000-7000 years ago – circumpolar migration arrival of Inuit and Na-Dené speakers found in the Arctic polar region and the Navajo in the Southwest who migrated from Alaska/Canada about 1400 CE
  • 5180 years ago (3180 BCE) – haplogroup O-FT11768, the common ancestor of Mr. Lynn and the Revels/Lewis group with many subgroups in the Philippines, Hawaii, Singapore, Brunei, China, Sumatra, and Thailand
  • 2244 years ago (244 BCE) – haplogroup O-CTS716, the common ancestor of Wesley Revels and Mr. Luo from Indonesia
  • The year 336 CE, 1684 years ago – haplogroup O-FTC77008, the common ancestor of Mr. Blount, UAE tester and a man from Thailand
  • 400 CE, 1600 years ago  –  Hawaii populated by Polynesian people
  • 760 CE, 1240 years ago – haplogroup O-F22410, common ancestor of Mr. Lynn with a Singapore man
  • 1492 CE, 528 years ago – Columbus begins his voyages to the “New World,” arriving in the Caribbean
  • By 1504 CE – European fishing began off of Newfoundland
  • 1565 – Spain claimed Guam and the Philippines
  • 1565 – Spanish trade between Manilla and Acapulco begins and continues for 250 years, until 1815, using crews of men from Guam, the Philippines, and enslaved people from the Marianas.
  • 1565 – St. Augustine (Florida) was founded by the Spanish as a base for trade and conquest along the eastern seaboard
  • 1566 – A Spanish expedition reached the Delmarva peninsula intending to establish a colony, but bad weather thwarted that attempt.
  • 1585-1587 – voyages of discovery by the English and the Lost Colony on Roanoke Island, North Carolina
  • 1603 – English first explored the Delmarva Peninsula, home to the Accomac people, now Accomack County, VA, where James Revels’s court record was found in 1667
  • 1607 – Jamestown, Virginia, founded by the English
  • 1608 – Colonists first arrived on the Delmarva Peninsula and allied with Debedeavon, whom they called the “laughing King” of the Accomac people. At that time, the Accomac had 80 warriors. Debedeavon was a close friend to the colonists and saved them from a massacre in 1622. He died in 1657.
  • 1620 – The Mayflower arrived near present-day Provincetown, Massachusetts
  • 1631-1638 – Dutch West India Company established a colony on the Delmarva Peninsula, but after conflicts, it was destroyed by Native Americans in 1638. The Swede’s colony followed, and the region was under Dutch and Swedish control until it shifted to British control in 1664
  • 1656 – Birth of James Revels, confirmed in a 1667 court record stating that he was an Accomack “Indian boy” from “Matomkin,” judged to be age 11, bound to Edward Revell. This location is on the Delmarva Peninsula.
  • 1741 CE –  Haplogroup O-BY60500 formation date that includes all of the Revels and Lewis testers who descend from James Revels born in 1656
  • 1765 – Whalers near Nantucket using crewmen from Hawaii (Sandwich Islands), Tahiti, and the Cape Verde Islands off of Africa
  • 1766 CE – Formation date for haplogroup O-FT45548, child haplogroup of O-BY60500, for some of the Lewis and Revels men who all descend from James Revels born in 1656
  • 1778 – Captain Cook makes contact with Hawaiian people
  • 1787 – The first male arrived in the Pacific Northwest from Hawaii
  • 1811 – Hawaiian seamen begin intermarrying with Native American females along the Pacific shore, eventually expanding their presence from Oregon to Alaska
  • 1839 – John Suter recruits Hawaiian men to travel with him to California
  • 1845 – Hawaiians employed by Fort Vancouver, with some marrying Native American women

Conclusions

It’s without question that James Revels was Native American very early in the settlement of the Delmarva Peninsula, now Accomack County, Virginia, but his common ancestor with Filipino men 5100 years ago precludes his direct paternal ancestor’s presence in the Americas at that time. In other words, his Revel male ancestor did not arrive in the Beringian indigenous migration 12,000-16,000 years ago. His ancestor likely arrived post-contact, based on a combination of both historical and genetic evidence.

Haplogroup O is not found in the Arctic Inuit nor the Na-Dene speakers, precluding a connection with either group, and has never been found in ancient DNA in the Americas.

Haplogroup O in the Revels lineage is most likely connected with the Spanish galleon trade with the Philippines and the early Spanish attempts to colonize the Americas.

The source of Haplogroup O in the Pacific Northwest group is likely found in the recruitment of Hawaiian men in the early/mid-1800s.

The Mexican Haplogroup O group likely originated with the Manilla/Mexico Spanish galleon trade.

The source of the Blount Haplogroup O remains uncertain, other than to say it originated in Thailand thousands of years ago and is also found in the UAE. The common Blount, UAE, and Thailand ancestor’s haplogroup dates to 336 CE, so they were all likely in or near Thailand at that date, about 1687 years ago.

What’s Next?

Science continuously evolves, revealing new details as we learn more, often clarifying or shifting our knowledge. Before the Discover tool provided haplogroup ages based on tests from men around the world, we didn’t have the necessary haplogroup origin and age data to understand the genesis of haplogroup O in the Americas. Now, we do, but there is invariably more to learn.

New evidence is always welcome and builds our knowledge base. Haplogroup O ancient DNA findings would be especially relevant and could further refine what we know, depending on the location, dates of the remains, who they match, and historical context.

Additional Big Y-700 tests of haplogroup O men, especially those with known genealogy or ancestor location, including Madagascar, would be very beneficial and allow the haplogroup formation dates to be further refined.

If you are a male with haplogroup O, please consider upgrading to the Big Y-700 test, here.

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Étienne Hebert (c1625-c1670): Two French Brothers & Their Ancient Ancestors – 52 Ancestors #413

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In the book, Les vielles familles d’Yamachiche: vingt-trois généalogies, v. 4 published in 1908 in Ontario, we discover that Étienne Hebert is one of two brothers who came from France and settled in Acadia, now Nova Scotia. Étienne married Marie Gaudet and Antoine Hebert married Genevieve LeFranc.

We know that Étienne and Antoine were brothers because in the 2nd marriage record for Jean-Jacques Hébert (1681-?) to Marguerite Leprince on April 27, 1734, at Saint-Charles-les-Mines, they were granted a dispensation from a 3rd degree consanguine relationship. The only overlap in their two family trees would be the parents of Étienne and Antoine Hebert.

Thank goodness for those church records.

Origins

Stephen A. White provided the following information about Étienne.

HÉBERT, Étienne, came from France with his wife Marie Gaudet, according to nine depositions: one from his grandson Jean Hébert (Doc. inéd., Vol. III, p. 11), one from Pierre Trahan, husband of his granddaughter Madeleine Comeau (ibid., p. 8), one from Pierre and Madeleine’s son Pierre Trahan (ibid., pp. 110-111) and one from their nephews Sylvestre and Simon Trahan (ibid., p. 30), two from husbands of Étienne’s great-granddaughters (ibid., Vol. II, p. 182; Vol. III, p. 90), one from a great-great-grandson (ibid., Vol. III, pp. 93-94), and two from husbands of his great-great-granddaughters (ibid., pp. 45, 92-93). Seven of these depositions name his wife as Marie Gaudet; only those of the two Pierre Trahans, father and son, do not.

Lucy LeBlanc Consentino documents these priceless depositions here.

Parents

There have been several proposed and presumed parents of Étienne and Antoine Hebert. None are proven, and some have been disproven. I’m not going to recount each theory here. I’ll briefly mention the most common ones and strongly suggest that anyone tempted to assign parents for these men consult existing resources and arguments first.

Tim Hebert’s website is no longer online, but you can view it here at Wayback Machine. Tim did an exceptional job documenting the various theories and Hebert descendants.

It has been said that possibly the brothers were from south of Loudon (LaChaussee, Martaize, etc.), however, since Charles Menou d’Aulnay’s family had land in that vicinity. If he recruited settlers from that area, there is a chance they came from there, but there is no proof of where they (or most other) Acadians came from. The linguistic studies by Genevieve Massignon tried to say that they were from the Loudon area, but perhaps she was focusing too much. It is probably true that they came from western France. But the lack of documentation in the Loudon region means that perhaps we’re looking in the wrong place. Michael Poirier has suggested they came from west of Loudon at the coast … near Baie de Bourgneuf.

He bases this on:
– the location of the monastery of the Assumption (on the island Chauvet), which was regularly attended by Richelieu and was the property of his brother, Alphonse.
– Port-Royal and the church of St Jean-Baptiste
– salt-water marshes in the area were drained … much like the dyke system utilized in Acadia
– it was a zone surrounded by Protestants and enclosing Catholics

Genevieve Massignon (1921-1966) argues that a number of familial alliances existed among the first Acadian settlers PRIOR to their arrival from France, pointing to a common French origin. She believes they lived in the Acadian Governor d’Aulnay’s seigneury in France near Loudun (comprised of the villages of Angliers, Aulnay, Martaizé, and La Chausée). The Hébert family was allied with the Gaudets through Étienne’s marriage to Marie. Marie’s sister Francoise was also allied with the Leblanc family through her marriage to Daniel. Evidence of their marriages in France is found in the Belle-Isle-en-Mer declarations in 1767. Moreover, a certain Jean Gaudet was censistaire in 1634 on land at Martaizé (Vienne) in the Seigneurie owned by the mother of Acadian governor Charles d’Aulnay. However, Massignon’s research failed to find any relevant baptismal or marriage records.

Another couple, Jacques Hebert and Marie Juneau have been debunked as parents, based on the date of their marriage and analysis by Stephen White. Jacques was found in Acadia 30 years before Étienne and Antoine, then moved into mainland Canada. It’s unlikely that his two sons would be found in Acadia and not near or with him. Not to mention the depositions that state that Étienne and Antoine were born in France.

Another parent candidate was Louis Habert who is generally considered to have been the first permanent settler in Canada, arriving in 1604. He married Marie Rolet in Paris in 1602 but wasn’t known to live in Acadia. Spelling variations of this family name include Hebert, Harbert, Herbert, Herbot, Harbelot, and others. You can read more about this at FamilySearch here.

One source stated that Stephen White reported that Etienne Hebert arrived on the ship, La Verge in 1648. Karen Theriot Reader, upon further examination, determined that the page given as the source does not in fact provide that information, nor elsewhere by White.

However, the Verve did arrive in 1648, chartered by Emmanuel LeBorgne, Sieur of Coudray, to transport supplies. No passenger list exists, and several ships arrived in Acadia over the years.

In a letter to Tim Hebert, Stephen White stated that their parents are “unknown.” No birth records have been found, and White found none of the proposed parents convincing or even probable.

We simply don’t know when and where Étienne and Antoine were born. It’s fair to say it was in France because families weren’t imported until 1636. The Hebert brothers were born in the 1620s. They would have been teenagers or young men in 1636.

What Was Happening in Acadia?

Warm up your tea or coffee, ‘cause this is a fascinating tale.

Acadia was truly the frontier and constantly caught in the middle in a tug of war between France and England for control of both the land and resources, along with the people.

Settlement in Acadia began in 1604, but we’re joining this history 28 years later.

In 1632, control of Acadia passed from the English back to the French, who immediately launched voyages transporting traders and workers, some of whom became settlers. Their initial goal wasn’t settlement, though, but trading posts.

Port Royal is shown on Champlain’s 1632 map.

Isaac de Razilly was a French noble sea captain and knight who convinced his cousin, Cardinal Richelieu, chief minister to the King of France, that colonizing and establishing fur trade with Acadia was a profitable business venture. As a bonus that probably sounded attractive to Richelieu, they could convert and baptize the Native people, too.

Razilly’s 1632 voyage on the L’Esperance a Dieu included about 300 people, mostly men with possibly 12-15 women. A French newspaper report from that time states that a third ship from Rochelle joined the other two. A mason, baker, nailmaker-blacksmith, joiners, gunsmiths, sawyers, laborers, and soldiers signed up.

In 1640, notarial records in La Rochelle, France, show many contracts of engagement for workers in Acadia, although most of those people aren’t shown in the 1671 census, meaning they either died or returned to France when their engagement was over. In 1640, at least 25 men and 5 women signed up.

Couillard-Despres in “Les Gouvernors” states that 63 men arrived on the Saint Clement in 1642 to assist Charles LaTour.

After Razilly’s death in 1635, his cousin, Charles de Menou d’Aulnay, de Charnisay prepared to take over the administration of Acadia. By this time, there were 44 inhabitants at Le Have, Razily’s base of operation. Sometime between 1635 and 1640, d’Aulnay moved the settlement to Port Royal, but the men who had married Native American women likely did not move with him.

However, Charles La Tour, who had lived in Acadia since he was 17 and was married to a Mi’kmaq woman, had other plans. His father, Claude, obtained a grant for Nova Scotia from the English king, and Charles was appointed Governor, serving from 1631-1642. In essence, the LaTour father-son duo had outsmarted d’Aulnay.

Workers still continued to arrive. The 1636 passenger list of the St. Jehan, including occupations and some location origins, still exists.

d’Aulnay and La Tour began as competitors, with LaTour working out of Cap Sable and the St. John River area with traders, and d’Aulnay, who moved the Acadian settlement from La Have to Port Royal, beginning cultivation. Given where we find Étienne Hebert living, he likely arrived with d’Aulnay.

However, the competition between those men soon became animosity, then open warfare, with both men claiming to be in charge of all of Acadia.

If you think there was no drama in a relatively unpopulated area, just try to keep this next bit straight.

In 1640, after LaTour’s Mi’kmaq wife died, he married a French Huguenot woman, Françoise-Marie Jacquelin, who had powerful connections.

In 1642, d’Aulnay had LaTour, a Huguenot, charged with treason against France. LaTour’s well-connected wife traveled to France to advocate on behalf of her husband, returning with a warship for him to defend himself.

Perhaps this was a bit hasty.

In the Spring of 1643, La Tour led a party of English mercenaries against the French Acadian colony at Port-Royal. His 270 Puritan and Huguenot troops killed three men, burned a mill, slaughtered cattle, and seized 18,000 livres worth of furs.

Apparently, LaTour was a traitor after all, at least from the French perspective.

LaTour then traveled to Boston seeking reinforcements from the English, and while he was gone, d’Aulnay seized all of his possessions and outposts, including Fort LaTour.

Are you keeping track of this? I think the score was 3 to 3 here, with a Hail Mary pass underway. Get the popcorn.

LaTour may have been traveling to Boston, but his wife, Françoise-Marie, had remained at home and was not about to relinquish Fort LaTour without a fight.

In the ensuing battle, Françoise-Marie, at the ripe old age of 23, defended Fort LaTour in the Battle of St. John for three days, using the warship. D’Aulnay lost 33 men but on the fourth day, was able to capture the fort. LaTour’s men were hung at the gallows as Françoise-Marie was forced to watch with a rope around her neck, just in case she got any bright ideas. She was clearly not a woman to be trifled with.

Françoise-Marie was not hung, but Nicolas Denys recorded in his journal that she died three weeks later as a prisoner in captivity. The cause remains unknown, but it’s safe to say that her death was a volley in war. 

After learning that his wife had died, his possessions confiscated, and his men killed, LaTour sought refuge in Quebec City. He did not return to Acadia for several years, but return he would – eventually.

For the time being, d’Aulnay was firmly in control, but that only lasted a few years.

In 1650, d’Aulnay drowned when his canoe overturned, which provided the opening LaTour had been waiting for. LaTour sailed to France, obtained royal favor, his property restored, and returned to Acadia as governor in 1653, accompanied by several new colonists, including Philippe Mius d’Entremont, 1st Baron of Pobomcoup.

It was about this time, around 1650, that Étienne Hebert married Marie Gaudet. Perhaps they hoped that living near her parents, a dozen miles upriver, would be more peaceful and less exposed to attack and conflict.

LaTour had remained a widower since his wife’s death defending Fort LaTour in 1645, but in 1653, he married…wait for it… d’Aulnay’s widow, Jeanne Motin. It was not a marriage in name only, as they had five children. Some said they married to heal the rift between the warring d’Aulnay and LaTour camps, some think it was simply a marriage of convenience for both, and others feel it was LaTour’s final victory over d’Aulnay. However, Jeanne was no shrinking violet because she evicted Nicolas Denys when he attempted to exploit d’Aulnay’s death by setting up trading posts at St. Ann and St. Peters.

LaTour wasn’t off the hook, though, because in an odd sort of way, d’Aulnay still managed to be a thorn in LaTour’s side – even from beyond the grave.

Along with d’Aulnay’s property and wife came his substantial debts to Emmanuel Le Borgne, his main financier from La Rochelle. There were two sides to this story because, as part of the deal, La Bourg and other seigneurs were supposed to recruit and transport new settlers to Acadia and care for them by building communal resources like mills and bake-ovens, but they didn’t.

It appears that the Acadians and their French sponsors were both relatively unhappy. The French did not live up to their end of the bargain by building mills and ovens, and consequently, the Acadians resisted paying taxes. Everyone resented the English, but the English needed the Acadian settlers to work the land. And, of course, the land passed back and forth between the French and English from time to time, punctuated by skirmishes and outright attacks.

Acadia, for an Atlantic peninsula of land with few people, was drama-central.

By 1653, it was estimated that there were 45-50 households at Port Royal and La Have, which provides us an estimate of 300-350 people, including 60 single men. Étienne Hebert was lucky to find a bride, any bride.

In 1654, Port Royal was still small, with approximately 270 residents, as estimated by pioneer Nicholas Denys. Denys was a French prisoner at Port Royal who had been responsible for recruiting volunteers for the 1632 Razilly expedition of 300 men from Rochelle, France. They landed at La Hève near modern Bridgewater, the eventual site of the Gaudet village. This location was near the upper reaches of the tidal portion of the Riviere du Dauphine, and their boat probably could not progress further.

Denys did us the favor of describing Port Royal in 1653:

There are numbers of meadows on both shores, and two islands which possess meadows, and which are 3 or 4 leagues from the fort in ascending. There is a great extent of meadows which the sea used to cover, and which the Sieur d’Aulnay had drained. It bears now fine and good wheat, and since the English have been masters of the country, the residents who were lodged near the fort have for the most part abandoned there houses and have gone to settle on the upper part of the river. They have made their clearings below and above this great meadow, which belongs at present to Madame de La Tour. There they have again drained other lands which bear wheat in much greater abundance than those which they cultivated round the fort, good though those were. All the inhabitants there are the ones whome Monsieur le Commandeur de Razilly had brought from France to La Have; since that time they have multiplied much at Port Royal, where they have a great number of cattle and swine.

The commentary about the French settling on the upper part of the river may be very important for the Hebert family because that’s exactly where they are found.

Denys also recorded that Robert Sedgewick of Boston had been ordered by Robert Cromwell to attack New Holland (New York). As Sedgewick prepared, a peace treaty was signed between the English and the Dutch. Since he was “all dressed up with nowhere to go,” he attacked Acadia in August 1654 and destroyed most of the settlements, including Port Royal, La Have, and the Saint John River village. Sedgewick left the area but appointed an Acadian council with Guillaume Trahan in charge. Some of the French may have returned to France at this point.

Denys doesn’t say if Sedgewick burned the upper river homesteads and farms or if he was satisfied with torching Port Royal. Living 12-14 miles away in the out-country may have been the saving grace of the Hebert and Gaudet families. Or, their homesteads and farms may have been destroyed, too. Certainly, if not burned out, they were devastated by Acadia falling to the English.

Acadia was back under English rule and would remain so until being returned, again, to the French in 1667.

After Sedgewick captured Acadia for the English, LaTour went to London to regain his property, again. Being a Protestant would have worked in his favor, as well as having led the English in raids against Port Royal in 1643.

In 1656, Cromwell granted property to two Englishmen and LaTour, but LaTour sold his share to the Englishmen and moved to Cap Sable, on the southern end of the peninsula, to attempt to live the rest of his life in peace.

We don’t know positively that the Hebert brothers were in Acadia at this time, but it’s almost assured. They had probably been in Acadia for between 10 and 30 years. If White is correct, they had resided in Acadia for eight years. Windows of immigration existed, but generally only when the French were in charge, although France imported settlers to other nearby parts of New France. The French were not imported directly into Acadia when the English ruled.

In 1666, France stopped sending colonists, ostensibly for fear of depopulating the mother-country. However, the English were still arriving in the colonies to escape religious prosecution and for economic reasons. Therefore, the Acadians were exposed to at least some English settlers, probably spoke and understood at least a little English, and established some level of trade with the English colonies along the Eastern seaboard.

By Mikmaq – Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=1351882

Given the 1671 census and the ages of his children, we know Étienne was married by 1651 and that his wife’s parents also settled in Acadia.

Life in Acadia always seemed to be contentious and apparently, in no small part, dangerous.

Étienne was probably in his mid to late 40s when he died, about 1670. He clearly didn’t die of old age, but probably as a result of hunting, fishing, or farming – some accident. Or, perhaps, there was a skirmish. It seemed like there was always some sort of skirmish, but a simple act of daily living such as fishing carried the risk of drowning.

The Catholic church records don’t exist, if they even had a priest at that time, so we don’t know when Étienne died. We can rest assured that, if possible, he was buried in the parish cemetery, now the Garrison Cemetery in Annapolis Royal, beside the fort and the Catholic church.

The First Acadian Census

Even though Acadia was officially returned to France in 1667, it didn’t actually happen right away. In 1670, the English surrendered the fort at Port Royal, apparently without incident. The new French governor arrived, bringing with him another 60 settlers and 30 soldiers. The new governor ordered a census, thankfully. He likely needed to know how many people would be paying taxes.

The first Acadian census was taken in 1671, documenting between 240 and 350  Acadian residents (depending which count you utilize) in 68 households in Port Royal and one household each in three other locations. Historians know some residents in settlements weren’t counted, and neither were Acadian/Native American families living with the Native people. Estimates of the entire Acadian population reach as high as 500.

Étienne was already deceased, but we can tell quite a bit from his widow’s census record, transcribed here by Lucy LeBlanc Consentino.

Marie Gaudet, widow of Étienne Hebert, 38. She has 10 children, two married children: Marie 20, Marguerite 19; Emmanuel 18, not yet married, Étienne 17, Jean 13, Francoise 10, Catherine 9, Martine 6, Michel 5, Antoine 1, 4 cattle, 5 sheep and 3 arpents of cultivated land.

This tells us that Etienne and Marie were married in about 1650, or maybe somewhat earlier. Their eldest living child was age 20. Étienne was probably about 25 years old when he married, so I’d estimate his birth year as 1625, give or take a few years. It appears that Marie Gaudet and her daughter, Marie Hebert, and her husband, Michel de Forest, and their families were probably living either on the same farm or even in the same house.

Marie’s youngest child was age 1, so we know that Étienne died sometime between 1669 and 1671.

His brother, Antoine Hebert is listed three houses away as a 50-year-old cooper, so he was born about 1621.

Hebert and Gaudet Allied Families

It’s clear that the Hebert family was somehow allied with the Gaudet family as early as 1650 when their children married. It’s possible that they married in France, or Acadia.

What we do know is that these two families lived in close proximity on the Riviere de Dauphine, now the Annapolis River.

This 1733 map at the Nova Scotia Archives is based on the 1707 census route and shows about a mile and a half or two miles distance between the Hebert and Gaudet homesteads – 57 years after Étienne Hebert and Marie Gaudet married.

Etienne Hebert lived along Bloody Creek, where the Hebert Village is found, courtesy of MapAnnapolis, below.

We know where Etienne, Marie, and their family lived and at least something about their life – but what else can we unearth?

The Hebert DNA Story

Eventually, the answer to where the Hebert brothers originated in France will be told through their Y-DNA, passed directly from father to son through the generations without ever being admixed with the mother’s DNA, or divided.

The Hebert family is well-represented in the Acadian AmerIndian Project with three Big-Y testers showing the same haplogroup. Haplogroup R-BY31006 was born about 1650, almost exactly when Étienne and his brother were marrying and having children near Fort Royal.

Click to enlarge any image

Two present-day project members descend from Étienne, and one descends from Étienne’s brother, Antoine. They have the same high-resolution haplogroup, so we know that their father had the same mutation that he gave to both sons. How I wish some Hebert men from France could test, but DNA testing for genealogy is illegal there.

Unfortunately, no other contemporary man of any surname is close to our Hebert cluster. The haplogroup ancestor upstream of R-BY31006 is the parent haplogroup R-BY31008 that occurred about 245 BCE, or 2245 years ago. The descendants of that man are also found in England, Norway, and Scotland, in addition to our Hebert men in France.

That’s quite interesting.

But there’s something even more interesting.

Ancient DNA

Looking at Ancient Connections in Discover, I note that one of the Hebert Ancient Connections was found in France and has been placed into haplogroup R-Z31644. I wonder what the connection is. Let’s take a look at that haplogroup.

The TimeTree shows us that nine ancient DNA samples are found on different haplogroup branches of R-Z31644, of which only one is found in Metz, France, and the rest in the British Isles. It’s unclear exactly what this means. Only the French sample and three others in England and Ireland are found in the current era, meaning after 1 CE. This was clearly prior to the Battle of Hastings in 1066, after which an influx of French settled in England.

Eight ancient DNA results are found in England, but none share a common ancestor earlier than 4300 years ago. Notably, one English burial from about 2000-2300 years ago shares a common ancestor with the Metz, France remains about 4000 years ago. The eight English remains, and our Metz guy descend from a common ancestor about 4300 years ago.

Did Étienne’s ancestors descend from the ancient sample at Metz? Maybe the study provides more clues.

According to the study’s authors:

The Sablon district, which is located in the southern part of the city of Metz, was, during the Gallo-Roman period, a huge necropolis where both inhumations and cremations are found. Towards the end of the 19th century, the exploitation of the sandpits enabled the uncovering of sarcophagi (stone), cists (brick and tile), coffins (wood) and vats (lead).

These characterise the new burial practices developed during late Antiquity. [Spans from about the 3rd to the 6th or 7th centuries.]

The largest funerary space spans almost a kilometre, on either side of the via Scarponensis (portion of the Reims/Metz road).

The Sablon area can be compared to the Collatina necropolis close to Rome by its chaotic organisation, although at a different scale

Looking at a map of Metz helps put this in context.

It’s unclear exactly where along this route the burials were discovered beginning in the late 1800s. They extend for more than a kilometer on both sides of the road in the Sablon neighborhood of Metz.

The Sablon neighborhood extends from near the old city center along the main artery that crosses railroad tracks that appear to sever the original road into the city.

Does the history of Metz tell us who lived there and what was occurring during this time? Indeed, it does.

Metz is located at the confluence of the Moselle and Seille rivers, near the junction of France, Germany, and Luxembourg. The original inhabitants were Celtic. The town was known as the “city of Mediomatrici,” a fortified city of the tribe by the same name.

The Mediomatrici village evolved into a Gallo-Celtic city after Julius Caesar conquered the Gauls in 52 BCE.

Named Divodurum Mediomatricum by the Romans, present-day Metz was integrated into the Roman empire in the first century CE, after which it was colloquially referred to as the Holy Village.

The historic district has kept part of the Gallo-Roman city with Divodurum’s Cardo Maximus, then called Via Scarponensis. Today, this is Trinitaires, Taison, and Serpenoise streets in the old city center, and the Decumanus Maximus, which is En Fournirue and d’Estrées streets. The Roman Forum was located at the Cardo and Decumanus intersection and is the Saint-Jacques Square today, as shown below.

By Alice Volkwardsen at German Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=10681319

The ancient burial occurred between 432 and 551 CE, as calculated from a molar and was found in a very large Gallo-Roman necropolis, more than a kilometer long, located on both sides along the old Roman road.

This cityscape shows Divodurum Mediomatricum in the second century CE, capital of the Mediomatrici, ancestor city of present-day Metz. The original Roman amphitheater is shown at far left, and the living quarters are located within the city walls, protecting them from attack. A wonderful summary of archaeological findings can be found here.

Today the the Centre Pompidou-Metzocation is found at the site of the original large Roman amphitheater. This amphitheater held upwards of 25,000 people and was the largest and most consequential amphitheater outside of Rome.

Rome’s influence ended when the city was attacked, pillaged and burned by the Huns on April 7, 451, then passed into the hands of the Franks about 50 years later. By 511, Metz was the capital of the Kingdom of Austrasia.

How Does the Metz Burial Connect to England?

How do the dots between Metz and the British Isles connect, given that the common ancestor of our Metz burial and the British Isles burials has descendants scattered throughout the British Isles and in Metz?

The Celts first migrated to the British Isles about 1000 BCE, or about 3000 years ago, so this ancient French man and the other ancient burials in the British Isles make sense. Their common ancestor lived 4300 years ago in Europe. The closest common ancestor of our Metz man and any English burial occurred 4000 years ago, 1000 years before the earliest Celtic migrations across the English Channel.

This man from Metz lived 1500 or 1600 years ago and shares an ancestor with several ancient British men in addition to our Hebert line and was likely Celtic..

Of course, not every Celtic man left Europe. Many stayed and eventually integrated with whoever the next conquering army was. That ensured survival. Metz was a prize to be won, controlled over the centuries by many masters.

We don’t know if this specific Celtic man buried along the Gallo-Roman Road was a direct ancestor to our Hebert line, but if not, they were assuredly related and shared common ancestors. The descendants of haplogroup R-BY31008 are unquestionably the ancestors of our Hebert line.

Back to Étienne

Étienne’s Y-DNA has identified his ancestors as Celtic some 4000 years, or 200 generations ago.

More recently, his Y-DNA confirmed his connection to Antoine Hebert, and the church records of both of their descendants confirmed them as brothers.

Depositions given by Étienne’s grandchildren, spouses of grandchildren, great-grandchildren, nieces, and nephews confirm that Étienne was born in France, but, unfortunately, does not say where. This information alone debunked some of his parent candidates.

We find no suggestion of his parents in Acadia, although that’s not impossible. Many people died and never made it into existing records. The Hebert brothers likely arrived together as young men. Antoine may have married in France, as his wife’s surname is not found in Acadia. Of course, her father could have died and left no record. Étienne’s wife’s family lives next to the Heberts in Acadia, but we don’t know if Étienne and Marie Gaudet married in France or after arrival in Acadia.

How well did Étienne remember France? Did he look over his slice of countryside along the Riviere du Dauphine, with its dikes holding the tidal river at bay, and think of similar dikes constructed by his ancestors in France?

What about his parents?

Did they die, or did he sail away, knowing he and his brother would never see them or their siblings again?

Did their family shrink into tiny dots on the horizon, waving from the wharf, then disappear forever?

Did the brothers leave because they wanted to, or did they leave perhaps because they had no family left? Often, orphans had few options in their home country, and any opportunity was welcomed.

Did Étienne marry Marie Gaudet in Acadia, or did they marry someplace in France, then two Hebert boys immigrating to the new land with the Gaudet family?

In one way, we know so much – that Étienne matches an ancient Celtic burial in Metz who died about 1500 years ago, with whom he shared a common ancestor about 4000 years ago – yet we can’t identify Étienne’s parents. At least not today, but hope springs eternal. Two years ago, we didn’t know this.

Hopefully, one day, DNA testing for genealogy will be available to men in France. Our answers lie in Hebert men in some small French village, probably along a river that was once a highway of history.

Acknowledgments

I’m incredibly grateful to the Hebert men who have taken the Big Y-700 DNA test at FamilyTreeDNA, and to FamilyTreeDNA, because without those tests and the Discover tool that includes ancient DNA connections, we would never be able to peer beyond the mists of time into their deep ancestry.

As more men test and more academic studies and ancient DNA results are added to the Discover database, we’ll continue to learn more. The Big-Y DNA test is the gift that just keeps on giving.

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Ancestry’s ThruLines Are a Hot Mess Right Now – But Here Are Some Great Alternatives

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Right now, ThruLines at Ancestry is one hot mess.

Aside from the inherent frustration, especially over a holiday weekend when many people had planned to work on their genealogy, I’d like to say, “don’t panic.”

I don’t have any inside information about what’s going on at Ancestry, and I’ve attempted to make contact through their support page with no luck. They make talking to a person exceedingly difficult; plus, it’s a holiday weekend, and they are probably inundated.

Regardless, I have an idea of what is happening. Ancestry has been in the midst of recalculating “things,” perhaps in relation to their other changes, which I’ll write about separately in a few days.

In any event, Ancestry SURELY MUST KNOW there’s a significant problem because I imagine thousands of their customers are screaming right about now. Adding another voice won’t be helpful.

Symptoms

  • You may not have ThruLines at all.
  • If you do have ThruLines, don’t trust the information, or more to the point, don’t trust that it’s in any way complete.

I have two tests at Ancestry, both connected to different trees so that my matches and Thrulines are calculated separately for each test.

Test One

My first Ancestry test is connected to my primary tree. I’ve been amassing Thrulines cousins ever since the feature was released. I have hundreds of cousin matches descended from some of my more prolific ancestors.

Additionally, my sister’s grandchildren have tested, as have other close relatives who have connected their tests to their trees.

Today, those people are still showing on my match list, but are NOT showing as matches in ThruLines. None of them. Most of my ThruLines ancestors are showing zero matches, and the rest are only showing very few. Ancestors who had hundreds before now have 2, for example.

Here’s an example with my cousin, Erik.

My grandfather, William George Estes, shown in Erik’s tree, above, is his great-grandfather. Erik is my half first cousin, once removed, and we share 417 cM over 16 segments.

Yet, looking at my ThruLine for William George Estes, neither he nor my other cousins are shown as matches. Same for William George’s parents, and so forth.

ThruLines is VERY ill right now.

Test Two

My second DNA test at Ancestry is even worse. There are no ThruLines calculated, even though my DNA is tree-attached, and I had ThruLines previously.

I see this message now, and I can’t even begin to tell you how irritating this is – in part because it suggests the problem is my fault. It’s clearly not. My tree hasn’t changed one bit. I’m not alone, either. I’ve seen other people posting this same message.

And yes, if you’re thinking that there is absolutely no excuse for this – you’re right.

However, outrage isn’t good for us and won’t help – so let’s all do something else fun and productive instead.

Productive Genealogy Plans

Here are some productive suggestions.

At MyHeritage:

At FamilyTreeDNA:

  • Build your haplogroup pedigree chart by locating people through different companies descended from each ancestor in your tree through the appropriate line of descent, and see if they have or will take a Y-DNA or mtDNA test.
  • Tests are on sale right now, and there’s no subscription required at FamilyTreeDNA for anything.
  • Check Y-DNA and mtDNA tests to see if there are new matches and if you share a common ancestor.

At 23andMe:

  • Check for new matches and triangulation.
  • Check to see if 23andMe has added any of your new matches to your genetic tree.

Remember, the parental sides are typically accurate, but the exact placement may not be, and 23andMe deals poorly with half-relationships. It’s certainly still worth checking though, because 23andMe does a lot of heavy lifting for you.

DNAPainter

For me, the most productive thing to do this weekend would be to copy the segment information from new matches with whom I can identify common ancestors at FamilyTreeDNA, MyHeritage and 23andMe – the vendors who provide segment data – and paint those segments to DNAPainter.

Not only does DNAPainter allow me to consolidate my match data in one place, DNAPainter provides the ability for me to confirm ancestors through triangulation, and to assign unknown matches to ancestors as well.

As you can see, I’ve successfully assigned about 90% of my segments to an ancestor, meaning I’ve confirmed descent from that ancestor based on my autosomal matches’ descent from that same ancestor – preferably through another child. Will new matches propel me to 91%? I hope so.

What percentage can you or have you been able to assign?

If you need help getting started, or ideas, I’ve written about DNAPainter several times and provided a compiled resource library of those articles, here.

Have fun!!!

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23andMe and GlaxoSmithKline Partnership Ends, Sparking Additional Layoffs

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23andMe has been slimming down. In April, they announced they were cutting about 75 jobs in their therapeutics division, equating to about 9% of their workforce, and now they have cut another 71 employees in response to the end of the five-year GSK partnership.

GenomeWeb reported the earlier and most recent 23andMe layoffs, along with a 6% revenue dip, here. 23andMe is a publicly held company and reported a net loss of $104.6 million.

In 2018, 23andMe partnered with GSK, GlaxoSmithKline, a British drug company, to jointly develop drugs based on the genomic profiles of their customers who choose to participate in this type of research. You may have noticed that 23andMe asks a wide variety of questions that genealogy testing companies typically don’t, and they also report on health and traits.

At the onset of the partnership, GSK made a $300 million equity investment in 23andMe. If you need to cure insomnia, you can read the SEC filing, here.

The original partnership was to last four years and could be extended for an additional 5th year, which it was, landing another 50 million dollars in the 23andMe coffers.

According to the press release by 23andMe and this 2020 blog article, the partnership has been successful, adding more than 40 genetically validated drug discovery programs to the GSK portfolio, making me wonder why the partnership was not extended.

Customers

The 23andMe page for medical professionals states that they have more than 12 million customers worldwide.

23and Me has stated several times that about 80% of their customers opt-in to research, which means that their de-identified DNA sequences are made available to both 23andMe and their selected partners for research purposes.

Accordingly, about 8 million people have opted-in to research.

If you’re doing the math, that means that:

  • 23andMe received $29.17 for each of their 12 million customers

Viewed another way:

  • 23andMe received $43.75 for each of their 8 million customers who are opted-in for research

Attempting to Increase Revenues

In the past several months, 23andMe has attempted to staunch the corporate blood flow by:

Neither of these moves have been well-received by genealogists.

Purchase Price

23andMe sells two types of tests. One is for both health and ancestry, and the second is for ancestry, aka genealogy, only.

  • The 23andMe Health and Ancestry test is currently priced at $229. The yearly membership costs an additional $69, for a total of $298, but the membership is currently free during the first year. That’s a lot for an autosomal test that only buys you up to 5000 matches.
  • The 23andMe ancestry-only test is $119, but comes with restrictions, including the 1500 match limit.

For comparison purposes, this article shows how many matches I have at each vendor.

If you want more than 1500 matches, you MUST PURCHASE the Health and Ancestry test, not the lower-cost genealogy-only test, plus the additional membership.

This is a very difficult pill to swallow (pardon the pun.) None of the other DNA testing companies limit your matches or charge for matching, and their prices right now for their autosomal tests are as follows:

Subscription aka Membership

In order to entice customers into purchasing subscriptions, called memberships, 23andMe allows up to 5000 matches instead of 1500. 23andMe has also limited additional features, taking them away from their original customers and putting them behind the subscription paywall.

In October 2020, when they implemented subscriptions, called memberships, along with these changes, they reduced their customers’ original match limit from 2000 to 1500. Of course, to receive more matches, you could purchase a new test and subscribe. No thank you.

In another attempt to throttle services to earlier customers, there were initially no ethnicity updates for people in October of 2020 who had tested on V2, V3 or V4 chips, although following public outcry, they reversed that position for at least the V3 and V4 customers. No other DNA testing company excludes customers from ethnicity updates. 

One cannot perform other functions, such as sort or filter by haplogroup on their site, unless you purchase the Health and Ancestry test, plus a membership. You can, however, download your matches and sort/filter that way..

What’s Next for 23andMe?

23andMe says they are now actively pursuing new big pharma partners.

I hope they can find their way forward. While I don’t often find relevant matches at 23andMe anymore, and I have an issue with their subscription policy, especially removing features from existing customers, they do have a pool of 12 million-ish people. These matches certainly help many people, especially because their health customers probably won’t have tested elsewhere.

Having said that, I can’t help but wonder how many of those 12 million are the same person multiple times because they’ve had to purchase multiple tests. I’ve purchased three for myself over the years, and I’m not purchasing a fourth – but I digress.

  • 23andMe is still a good site for matching, especially for adoptees or people seeking unknown family members. You can also see how your matches match each other. You just never know where that critical match is going to pop up.
  • 23andMe provides painted ethnicity chromosome segments, along with FamilyTreeDNA. In my opinion, they are the top two vendors for ethnicity accuracy.
  • 23andMe and FamilyTreeDNA both report X-DNA matching, which can be very useful.
  • 23andMe is still the only vendor to construct a genetic tree – and yes – I know it’s not always completely accurate. Still, their tree creation is innovative and automated – based on how you match people and how they match each other. For adoptees and people seeking parents or grandparents, it’s essential because they start with nothing.
  • 23andMe doesn’t allow customers to upload or create a family tree, so you can’t view the family tree of your matches to find a common ancestor. You can include a link to your online family tree in your Enhanced Profile under Settings, but many people never see this, or aren’t genealogists.

Unfortunately, 23andMe is not focused on genealogy – at all. Their focus has always been medicine and health. From their perspective, genealogists are candidates to opt-in for genetic research, but that doesn’t mean genealogists can’t still benefit – even if we don’t opt-in, don’t purchase the more expensive $229 Health and Ancestry test, and don’t purchase their membership.

If you’re interested in more recent relatives, 23andMe is great because the 1500 match limit won’t impact you at all. Closer relatives will cluster at the top of your match list.

If you’re looking for matches that descend from more distant ancestors, you may find it worthwhile to purchase the more expensive test and the membership, at least for one year.

Filtering/Sorting Restriction Workaround 

While there’s no way around the 1500 or 5000 match limit, except that 23andMe won’t roll someone off of your match list if you’ve communicated with them, or tried to, there is a workaround for the restrictive filtering.

I check my matches periodically, sorting by the newest matched relatives. I also download my match list occasionally. I find it easier to review the information in spreadsheet format because I can search for surnames, locations, haplogroups and other information much more easily than online, especially given the restrictive filters.

However, when you download your match list, that information is downloaded as well.

Be sure to record notes on each match at 23andMe when you discover relevant information by clicking on the match and scrolling to the very bottom of the page. Your notes at 23andMe are downloaded onto the spreadsheet along with the rest of their information.

The instructions for downloading your match list, which is NOT the same as downloading your DNA file, are contained in this article. Give it a try!

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Haplogroups: DNA SNPs Are Breadcrumbs – Follow Their Path

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Recently a reader asked some great questions.

If Y-DNA is unchanged, then why isn’t the Y-DNA of every man the same today? And if it’s not the same, then how do we know that all men descend from Y-Adam? Are the scientists just guessing?

The scientists aren’t guessing, and the recent scientific innovations behind how this works is pretty amazing, so let’s unravel these questions one at a time.

The first thing we need to understand is how Y-DNA is inherited differently from autosomal DNA, and how it mutates.

First, a reminder that:

  • Y-DNA tests the Y chromosome passed from father to son in every generation, unmixed with any DNA of the mother. This article focuses on Y-DNA.
  • Mitochondrial DNA tests the mitochondria passed from mothers to all of their children, but is only passed on by the females, unmixed with the DNA of the father. This article also pertains to mitochondrial SNPS, but we will cover that more specifically later in another article.
  • Autosomal DNA is passed from both parents to their children. Each child inherits half of each parent’s autosomal DNA.

Let’s look at how this works.

Autosomal vs Y-DNA Inheritance

Click on image to enlarge

Autosomal DNA, shown here with the green (male) and pink (female) images, divides in each generation as it’s passed from the parent to their child. Each child inherits half of each parent’s autosomal DNA, meaning chromosomes 1-22. For this discussion, each descendant shown above is a male and has a Y chromosome.

This means that in the first generation, which would be the great-grandfather, about 700,000 locations of his green autosomal DNA are tested for genealogy purposes.

His female partner (pink) also has about 700,000 locations. During recombination, they each contribute about 350,000 SNPs (Single Nucleotide Polymorphisms) of autosomal DNA to their child. Their offspring then has a total of 700,000 SNPs, 350,000 green and 350,000 pink contributed by each parent.

This process is repeated for each child, whether male or female (with the exception of the X chromosome, which is beyond the scope of this article), but each child does not receive exactly the same half of their parents’ autosomal DNA. Recombination is random.

In the four generations shown above, the green autosomal DNA of generation one, the great-grandfather, has been divided and recombined three times. The original 700,000 locations of great-grandfather’s green DNA has now been whittled down to about 87,500 locations of his green DNA.

Y-DNA in the Same Generation

Looking now at the blue Y-DNA at left, the Y-DNA remains the same in each generation with the exception of one mutation approximately every two or three generations.

As you can see in the chart, in the exact same number of generations, the Y-DNA of each male, which he inherited from his father:

  • Never recombines with any DNA from the mother
  • Never divides and gets smaller in subsequent generations
  • Remains essentially unchanged in each generation

The key word here is “essentially.”

Y-DNA

The Y chromosome consists of about 59 million locations or SNPs of DNA. STR tests, Short Tandem Repeats, which are essentially insertions and deletions, test limited numbers of carefully curated markers selected for the fact that they mutate in a genealogically relevant timeframe. These markers are combined in panels of either 67 or 111 marker tests available for purchase at FamilyTreeDNA today, or historically 12, 25, 37, 67, and 111 marker panels. The STR test was the original Y-DNA test for genealogy and is still used as an introductory test or to see if a male matches a specific line, or not.

From the STR tests, in addition to matching, FamilyTreeDNA can reliably predict a relatively high-level haplogroup, or genetic clan, based on the frequency of the combinations of those marker values in specific STR locations.

SNPs are much more reliable than STRs, which tend to be comparatively unstable, mutating at an unreliable rate, and back mutating, which can be very disconcerting for genealogy. We need reliable consistency to be able to assign a male tester to a specific lineage with confidence. We can, however, find genealogically relevant matches that may be quite important, so I never disregard STR tests or testers. STR tests aren’t relevant for deeper history, nor can they reliably discern a specific lineage within a surname. SNP tests can and do.

The Big Y-700 SNP test gives us that and more, along with the earlier Big Y-500 test which scanned about 30 million locations. The Big Y-700 is a significant improvement; men can upgrade from the Big Y-500 or STR tests.

The Big Y-700 test scans about 50 million Y-DNA locations, known as the gold standard region, for all mutations. It reports 700 or more STR markers for matching, but more importantly, it scans for all SNP mutations in those 50 million locations.

All mutations are confirmed by at least five positive repeat scans and are then assigned a haplogroup name if found in two or more men.

Y-DNA Testing

If Y-DNA remained exactly the same, then the Y-DNA of men today would be entirely indistinguishable from each other – essentially all matching humankind’s first common ancestor. With no changes, Y-DNA would not be useful for genealogy. We need inherited mutations to be able to compare men and determine their level of relatedness to each other.

Fortunately, Y-DNA SNPs do mutate. Y-DNA is never divided or combined, so it stays essentially the same except for occasional mutations which are inherited by the following generations.

Using SNP markers scanned in the Big Y test, one new mutation happens on the average of every two or three generations. Of course, that means that sometimes there are no mutations for a few generations, and sometimes there are two mutations between father and son.

What this does, though, very effectively, is provide a trail of SNP mutations – breadcrumbs essentially – that we can use for matching, AND for tracking our mutations, which equate to ancestors, back in time.

Estes Male Breadcrumb Trail

I’ve tested several Estes men of known lineage, so I’m going to use this line as an example of how mutations act as breadcrumbs, allowing us to track our ancestors back in time and across the globe.

Multiple cousins in my Estes line have taken the Big Y-700 test.

My closest male cousin matches two other men on a unique mutation. That SNP has been named haplogroup R-ZS3700.

We know, based on our genealogy, that this mutation occurred in Virginia and is found in the sons of Moses Estes born in 1711.

How do we know that?

We know that because three of Moses’s descendants have tested and all three of those men have the same mutation, R-ZS3700, and none of the sons of Moses’s brothers have that mutation.

I’ve created a chart to illustrate the Estes pedigree chart, and the haplogroups assigned to those men. So, it’s a DNA pedigree chart too. This is exactly what the Big-Y DNA test does for us.

In the red-bordered block of testers, you can see the three men that all have R-ZS3700 (in red), and all are sons of Moses born in 1711. I have not typed the names of all the men in each generation because, for purposes of this illustration, names aren’t important. However, the concept and the fact that we have been able to connect them genealogically, either before or because of Y-DNA testing, is crucial.

Directly above Moses born in 1711, you can see his father Abraham born in 1647, along with Moses’ brothers at right and left; John, Richard, Sylvester, and Elisha whose descendants have taken the Big Y-700 test. Moses’s brothers’ descendants all have haplogroup R-BY490 (in blue), but NOT R-ZS3700. That tells us that the mutation responsible for R-ZS3700 happened between Abraham born in 1647, and Moses born in 1711. Otherwise, Moses’s brothers would have the mutation if his father had the mutation.

Moses’s descendants also have R-BY490, but it’s NOT the last SNP or haplogroup in their lineage. For Moses’s descendants, R-ZS3700 occurred after R-BY490.

You can see haplogroup R-BY490 boxed in blue.

We know that Moses and his father, Abraham, both have haplogroup R-BY490 because all of Abraham’s sons have this haplogroup. Additionally, we know that Abraham’s father, Silvester also had haplogroup R-BY490.

How do we know that?

Abraham’s brother, Richard’s descendant, tested and he has haplogroup R-BY490.

However, Silvester’s father, Robert born in 1555 did NOT have R-BY490, so it formed between him and his son, Silvester.

How do we know that?

Robert’s other son, Robert born in 1603 has a descendant who tested and has haplogroup R-BY482, but does NOT have R-BY490 or R-ZS3700.

All of the other Eates testers also have R-BY482, blocked in green, in addition to R-BY490, so we know that the mutation of R-BY490 developed between Robert born in 1555 and his son, Silvester born in 1600, because his other son’s descendant does not have it.

Looking at only the descent of the haplogroups, in order, we have

  • R-BY482 (green) found in Robert born in 1555 and all of his descendants.
  • R-BY490 (blue) found in Silvester born in 1600 and all of his descendants, but not his brother
  • R-ZS3700 (red) found in Moses born in 1711 and all of his descendants, but not his brothers

If we had Estes men who descend from the two additional documented generations upstream of Robert born in 1555, we might discover when R-BY482 occurred, but to date, we don’t have any additional testers from those lines.

Now that we understand the genesis of these three haplogroups in the Estes lineage, what else can we discover through our haplogroup breadcrumbs?

The Discover Reports

By entering the haplogroup in the Discover tool, either on the public page, here, or clicking on Discover on your personal page at FamilyTreeDNA if you’ve taken the Big-Y test, you will see several reports for your haplogroup.

I strongly suggest reviewing each category, because they cumulatively act as chapters to the book of your haplogroup story, but we’re going to skip directly to the breadcrumbs, which is called the Ancestral Path.

The Ancestral Path begins with your haplogroup in Line 1 then lists the first upstream or parent haplogroup in Line 2. In this case, the haplogroup I entered is R-ZS3700.

You can see the estimated age of the haplogroup, meaning when it formed, at about 1700 CE. Moses Estes who was born in 1711 is the first Estes man to carry haplogroup R-ZS3700, so that’s extremely close.

Line 2, R-BY490 occurred or was born about 1650, and we know that it actually occurred between Robert and Silvester born in 1600, so that’s close too.

Scanning down to Line 3, R-BY482 is estimated to have occurred about 1500 CE, and we know for sure it had occurred by 1555 when Robert was born.

We see the parent haplogroup of R-BY487 on Line 4, dating from about 750 CE. Of course, if more men test, it’s possible that more haplogroups will emerge between BY482 and BY487, forming a new branch. Given the time involved, those men wouldn’t be expected to carry the Estes surname, as surnames hadn’t yet been adopted in that timeframe.

Moving down to Line 9, we see R-ZP18 from 2250 BCE, or about 4250 years ago. Looking at the right column, there’s one ancient sample with that haplogroup. The location of ancient samples anchors haplogroups definitively in a particular location at a specific time.

Haplogroup by haplogroup, step by step, we can follow the breadcrumbs back in time to Y-Adam, the first homo sapiens male known to have descendants today, meaning he’s the MRCA, or most recent common ancestor for all men.

Neanderthals and Denisovans follow, but their Y-DNA is only available through ancient samples. They have no known direct male survivors, but someday, maybe someone will test and their Y-DNA will be found to descend from Neanderthals or Denisovans.

Now that we know when those haplogroups occurred, how did our ancestors get from Africa 232,000 years ago to Kent, England, in the 1400s? What path did they take?

The new Globetrekker tool answers that question.

The Breadcrumb Trail

In Globetrekker, each haplogroup’s location is placed by a combination of testers’ results, their identified earliest known ancestor (EKA) country and location, combined with ancient samples, climatic factors like glaciers and sea levels, and geographic features. You can read about Globetrekker here and here.

To view the Globetrekker tool, you must sign it to an account that has taken the Big Y test. It’s a tool exclusively provided for Big-Y testers.

You can click at the bottom of your Globetrekker map to play the animated video.

Beginning in Africa, our ancestors began their journey with Y-Adam, then migrated through the Near East, South Asia, East Asia, then west through central Asia into Europe. The Estes ancestors crossed the English Channel and migrated around what is now England before settling in Deal, on the east coast.

Clicking on any haplogroup provides a description of that haplogroup and how it was placed in that location.

Enabling the option for ancient DNA shows those locations as well, near the haplogroups they represent when the animation is playing.

Clicking on the shovel icon explains about that particular ancient DNA sample, what is known, and how it relates to the haplogroup it’s connected to by a dotted line on the map.

Pretty cool, huh!!

End to End

As you can see from this example, Big Y results are an end-to-end tool.

We can use the Big Y-700 haplogroups very successfully for recent genealogy – assigning testers to specific lines in a genealogy timeframe. Some haplogroups are so specific that, without additional information, we can place a man in his exact generation, or within a generation or two.

Not shown in my Estes pedigree chart is an adoptee with a different surname, of course. We know that he descends from Moses’s line because he carries haplogroup R-ZS3700, but we are still working on the more recent generations using autosomal DNA to connect him accurately.  If more of Moses’s descendants tested, we could probably place him very specifically. Without the Big Y-700 test, he wouldn’t know his biological surname or that he descends from Moses. That’s a HUGE breakthrough for him.

There’s more about the Estes line to learn, however.

If our Estes cousins tested their brothers, uncles or other Estes males in their line, they would likely receive a more refined haplogroup that’s relevant only to that line.

Using Big-Y test results, we can place men within a couple of generations and identify a common ancestor, even when all men within a haplogroup don’t know their genealogical lineage. Using those same test results, we can follow the breadcrumbs all 50 steps back in time more than 230,000 years to Y-Adam.

End to end, the Big-Y test coupled with breadcrumbs in Discover, Globetrekker, and other amazing tools is absolutely the most informative and powerful test available to male testers for their paternal line genealogy.

These amazing innovations tracking more than 50,000 haplogroups across the globe answer the original questions about how we know.

The more people who take or upgrade to the Big Y-700 test, the more haplogroup branches will be added, and the more refined the breadcrumbs, ages, and maps will become. In other words, there’s still more to learn.

Test if you haven’t, and check back often for new matches and breadcrumbs, aka updates.

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Globetrekker – A New Feature for Big Y Customers From FamilyTreeDNA

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FamilyTreeDNA recently released Globetrekker, a great new feature for Big Y customers as part of the Discover tools. You can read about the Discover tools, here.

What Is Globetrekker?

Globetrekker is a new mapping feature that maps your Y-DNA ancestral migration path from Y-Adam in Africa born about 200,000 years ago to where your direct paternal ancestors are found most recently based on:

  • The earliest known ancestor (EKA) locations of you, your matches and other testers
  • Ancient DNA samples
  • Various geographic criteria including elevation, migration corridors, sea levels, and glaciers.

This data-driven model also includes sea levels over time and some climate factors, such as glaciation. Clearly, our ancestors needed access to clean water, food and an environment where they weren’t going to freeze to death. If they had to choose between migrating along a lower level coastal region, or heading straight across the high mountains into the unknown, it’s more likely that they took the lower elevation coastal route with assured food.

Globetrekker displays the “most likely” corridors for you to review.

While you only see your Y-DNA line initially, the map includes 48,000 migration paths for all haplogroups spread across each continent. If you’ve taken the Big Y test, you can view any of the haplogroups in Discover.

And, there’s an integrated tree browser, too.

You can read FamilyTreeDNA’s blog article, written by Goran Runfeldt, head of R&D, here.

Please Note

  • Everyone must sign into their own account to use the new Globetrekker tool. To use the rest of the Discover features, everyone can use the public version of the tool, but Globetrekker is for Big Y customers only, which is why you need to sign in. You’ll also receive more information in other categories, such as Notable and Ancient Connections, if you access Discover through your account. The free public version is limited.
  • If you’re a project administrator and you normally view your project members’ results through your project (with member-granted authorization, of course) you can’t do that yet with Globetrekker.
  • This means that every tester has to sign on using their own kit number and password. FamilyTreeDNA is working on Group Administrator access, so don’t despair if you normally depend on your volunteer administrator to handle things for you and explain. It’s coming.
  • The migration map includes only pre-Columbian migrations. In other words, if your EKA is not Native American and is brick-walled in the US, you won’t see it on the map. You’ll see your closest haplogroup location before about 1500.
  • These routes will change over time with additional testers whose results will shift and refine the paths.

Best Thing You Can Do

The best things you can do, aside from taking (or upgrading to) a Big Y-700 test are:

  • Complete your earliest known ancestor (EKA) information.
  • Be SURE to include a country AND a location of origin because that’s the data Globetrekker draws from.
  • If your cousins test too, you may be assigned a new, more refined haplogroup, so recruit people. If you don’t know anyone specific, looking at your STR matches is a good resource to find candidates.

Adding Your EKA

To add your EKA and their geographic location, sign in to your account and click on your name, which will display a menu.

Select Account Settings.

Select Genealogy, then Earliest Known Ancestors, then complete the information, including Country, which assigns the flag, among other things. Click on update location to complete or change this location.

Search or place the pin in the correct location. Then click Save.

There are three very important pieces of EKA information that need to be completed to reap all the benefits of the Matches Map, Discover, the Time Tree, the Group Time Tree that includes ancestors, and Globetrekker.

  1. EKA Name and birth/death date
  2. Country of Origin field using the dropdown (Please note Native American entries for proven Native ancestors/haplogroups)
  3. Ancestral Location for specific locations for the Matches Map

While you’re here, enter your direct matrilineal ancestor’s information too – that’s your mother’s mother’s mother’s line, which you’ll need for mitochondrial DNA..

Then, click the orange Save button at the bottom of the page.

Your map location will also appear on your STR Matches Map. You may find relevant matches there, even if they haven’t taken the Big Y test.

There’s immense power in collaboration.

I often reach out to STR panel (12-111 markers) matches and men with the same or similar surnames, asking if they will consider upgrading to the Big Y, sometimes providing testing scholarships. The only way to obtain the most refined haplogroup possible and the most accurate migration path is for multiple people in the same lineage to test AND complete the location information.

Now that we’ve completed our housekeeping, let’s look at Globetrekker.

Globetrekker Quick Test Drive

I’ll be writing about Globetrekker results in detail soon, but for right now, let’s just take a quick spin.

Click on any image to enlarge

Sign in to your account and click on the Discover Haplogroup Reports under Y-DNA Results and Tools.

You’ll see your Haplogroup Story, of course, and on the left side, you’ll see the Globetrekker link. Click on Globetrekker.

It Takes Two to Tango

Please note the introduction at the top of the Globetrekker page, and don’t get drawn into the beautiful map without reading this part first, along with the Release Announcement, Caveats, and Survey. Please take the survey after you’ve used Globetrekker.

Click on image to enlarge

  • In order to RECEIVE a detailed haplogroup, it takes at least two people with the variant (mutation) that is then named and becomes the same haplogroup. This is why we recommend that men ask a cousin from the same paternal line to test, or even a father/brother/uncle.
  • To MAP the location of a haplogroup on Globetrekker, it takes at least two people with the same haplogroup who have selected a location. Looking at my cousin’s results, I had already entered his EKA and location, but apparently his Big Y matches have not, so there are not two men with R-ZS3700 who have locations specified. I need to contact his matches.

Be sure to enter all of your EKA info. If your cousins have tested, they need to enter their information as well.

  • Globetrekker cannot use results for the mapping function without locations.
  • Globetrekker cannot use non-Native American haplogroups that are recorded with a location in the Americas. Globetrekker does provide Native American mapping in North and South America when the haplogroup is Native and a location is provided.
  • Globetrekker CAN utilize coordinates in the Americas, but a country of origin in Europe or elsewhere pre-Columbus. Globetrekker defaults to the country of origin. Please make sure this information is accurate and not just a guess or oral history.

Locations or at least countries need to be as accurate as possible. If there are only two men with a specific haplogroup, for example, and one enters England and the other enters France, Globetrekker tries to plot the location of that haplogroup someplace in the middle. In this circumstance, probably neither person is happy – both complaining about inaccuracy. Yet another reason why it’s a good thing to help your fellow genealogists.

Therefore, if you notice that you have a Big Y match on either your Big Y match list, or your STR (12-111 panel) matches, and they don’t have an EKA and country listed, with a location displayed on the matches map, PLEASE email them and ask nicely if they will add that info. You can send them a link to this article to explain why providing that information is critically important for them AND the people they match, just like your information is crucial to them. Without location data, Globetrekker paths can’t be calculated correctly, and sometimes not at all. The more data, the greater the accuracy.

After you enter your EKA information and after Big Y results are back, it will be a week or so before Discover and Globetrekker are up to date. Discover is updated weekly, and if a new haplogroup is added, Globetrekker will be up to date the following week.

Drum Roll Please…..

Here it is. The new highly refined Globetrekker migration map. It’s a beauty!

Your end-of-line haplogroup, or the closest one that can be calculated, will be shown in orange. In this case, it’s R-BY490 (circa 1650 CE) because the location of R-ZS3700 (circa 1700 CE) can’t be calculated.

On the map, you can see the various haplogroups that are upstream of haplogroup R-BY490, meaning parent haplogroups.

The path from Y-Adam in Africa is mapped, with the color changing to represent the birth of each major haplogroup in the migration path.

For example, I clicked on the pin for haplogroup CF, which expanded that haplogroup to CF-P143 and showed information about how the haplogroup pin was located on the map – plus the age and sea level difference at the time.

Scroll down on the map until you see the play button. Clicking on that button animates the migration path, beginning with Y-Adam, then progressing to the most current pre-Columbian migration.

In this case, I paused the video at the formation of haplogroup R1.

Notice the glaciation that both forms and recedes. Clearly, your ancestors weren’t living there during glaciation, but humans moved into those areas after the glaciers thawed and retreated.

You may be surprised at the path your ancient ancestors took, so I encourage you to spend some time with this map, reviewing the approximate path and your parental haplogroups with an open mind.

A legend is located in the far right upper corner to help explain the map details, including Ocean Currents and the various sea level colors.

Notice Doggerland, in dark green, which was a land mass when some haplogroups arrived in what is now the British Isles. Doggerland flooded sometime between 6500 and 6200 BCE, or about 8500 years ago, so it’s sea today. In other coastal locations, some previous land areas are covered by water today. Note the Baltic above, for example. Truthfully, that explains a lot. I knew about Doggerland but not about many of the other coastal regions around the world.

Pay close attention to what’s happening on the map. I noticed that my red pin for the current haplogroup is found in Deal, England, but so is an earlier haplogroup, so the later pin obscures the earlier pin. I enlarged the map and paused the video at 1400 CE so the red pin doesn’t form yet, then clicked on haplogroup R-Z290 that arrived from across the English Channel.

The R-Z290 pin location tells me that my Estes male ancestors arrived from continental Europe around 4650 years ago. My assumption (there’s that word again) had been that the original Estes ancestors arrived, then stayed right in Deal, a coastal village very near Dover, the closest point to the European mainland. According to Globetrekker, that wasn’t at all what happened.

I was initially somewhat skeptical, but then looking at all of the upstream haplogroups, I realized that those 17 haplogroups upstream of R-BY490 had to get into the other parts of the British Isles somehow – and my ancestor clearly descends from those men.

Could my ancestors have crossed back over to the European mainland at some point, then recrossed into Deal? Yes, of course, but without any genetic or other evidence, that’s speculation ONLY, with nothing at all to support it. In other words, that speculation would be based on what I believed all these years and nothing more.

The data-driven genetic scientific evidence tells us that our Estes ancestor arrived in what is today England about 4500 years ago. As you can see, there are a total of 17 points in England that have been reliably placed, not just one or two that might be open to speculation. Additionally, we have ancient DNA evidence.

Notice the functions at the top of the map. Turn on Ancient Connections. You’ll see the little shovels appear when their timeframe and location are relevant to the map migration, then disappear when it isn’t.

Pause the map again, and click on the shovel to display relevant information about the archaeology dig that produced Y-DNA results of sufficient quality to be included. Those ancient samples often anchor haplogroups in a known place at a specific time.

While you’re enjoying different views, try the other options at the top of the Globetrekker map.

Integrated Tree Browser

Scroll down beneath the map to view the integrated tree browser.

This is VERY cool because the tree browser moves in tandem with the map above.

You can see that the migration map shows R-BY487, and on the timeline below, R-BY487 is showing at the top, along with the downstream haplogroups.

R-BY482 (circa 1500 CE), R-BY490 (circa 1650 CE), and R-ZS3700 (circa 1700 CE) are all Estes surname haplogroups. Prior to that, R-BY487 (circa 750 CE) has no associated surname. Surnames hadn’t been adopted yet, but we know approximately where they were living just the same. We can now reference the appropriate historical period in England to determine what was happening when they lived there.

Why the Big Y?

The Big Y test does five things extremely well:

  1. Scans millions of locations on the Y chromosome looking for mutations that, when compared with other Big Y testers, places men conclusively on their branch, and sometimes on their twig and leaf of the Y-DNA haplotree. Men carrying previously undiscovered mutations from the same line establish a newly named haplogroup.
  2. Unambiguously matches testers with men who descend from a common ancestor. SNPs, the mutations measured in the Big Y test are not subject to back-mutations and other occasional instabilities that plague the STR markers in the 12-111 panel tests.
  3. Provides matching to both STR and SNP markers, allowing genealogical connections to men who have taken either type of test. Some people who have taken STR tests have either chosen not to upgrade (yet) or may have passed away. With the Big Y test, those legacy tests, some of which are more than 20 years old, are still useful.
  4. Provides an estimated date of when the common ancestor lived.
  5. Reaches reliably back in time, before the age of surnames, allowing testers to peer into the past based on a combination of genetics and history.

In other words, the Big Y test provides the best of both worlds, genealogy for close surname matches and anthropology for ancient matching and migration.

Lots to Explore

Globetrekker results are available to men who took either the Big Y-500 or the Big Y-700. Those who took the Big Y-500 can upgrade for significantly more refinement and potentially new haplogroups. Men who have not yet tested, or who just ordered one of the STR panels can upgrade to learn about your matches, your haplogroup, and the migration path through history your ancestor trod to arrive where your EKA lived.

I’m looking forward to reviewing all of the kits I manage that have taken the Big Y test. Let me know what you think about your Globetrekker results, and be sure to complete the survey and let FamilyTreeDNA know too.

If you’d like to learn more about your Big Y results, be sure to check out both Discover and Globetrekker. Discover is public, but Big Y testers will receive more information. Globetrekker is for Big Y customers only.

Remember, both will change as more people test and new results come in, so check back often.

The FamilyTreeDNA Big Y Facebook Group

A few weeks ago, FamilyTreeDNA introduced their FamilyTreeDNA Big Y Group on Facebook. As of today, just shy of 8000 people have joined. You do have to agree to follow the rules, but you don’t need to have taken a Big Y test. Lots of people join to learn, including many women who manage Y-DNA tests for family members or people who just want to understand more about one of the three types of tests for genetic genealogy.

You’re welcome to join too, here.

The Summer Sale

Several people have asked when the Big Y or the upgrades would be on sale. The summer sale runs from August 1-31, and all Y-DNA tests and upgrades are included, here.

If you’ve already taken one of the STR panel tests, or the Big Y-500, the Big Y-700 is less expensive when you upgrade. Just sign in to your account and click on the orange Add Ons and Upgrades button at the top right of your page, then on “Upgrades.”

Click here to purchase or upgrade.

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FamilyTreeDNA’s New Big-Y Group on Facebook

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FamilyTreeDNA recently created a private Big Y Facebook group.

This group is different than others because it’s focused solely on the Big Y product and how to use it for genealogy, and it’s run by FamilyTreeDNA. Additionally, several knowledgeable community members are administrators and moderators. (Full disclosure – I’m a moderator.)

The new group has been around for only a few days, and already there are some very interesting postings.

For example, I asked earlier in the day how people go about recruiting men, especially from other countries, to take a Big Y test. Everyone who lives in a diaspora region wants to know where their ancestors are from. We want to connect with our homeland, find their communities, and, if we are lucky, walk in their footsteps.

In the past four hours, there have been 13 very well-thought-out responses that include several ideas and examples. I encourage you to join and participate. Here’s the link to the Big Y group, and here’s the link to that posting. Perhaps you have an idea to add, you need ideas, or both.

You don’t have to be a male or to have taken a Big Y test to join, but you do have to answer questions. Please don’t invite your friends to join because people must request to join themselves to be able to answer the mandatory questions.

Anyone who does not answer all of the questions will not be approved. We are trying to restrict admission to non-trolls and people who are actually interested in the topic at hand to assure a good experience for everyone.

I’m very excited about this new educational opportunity. It’s unusual for a vendor to create a resource where you are interacting with their staff, so take a Big Y test, or purchase one for your toughest lineage, and join the Big Y Group. Please either use this direct link to join, or be sure you are joining the group with the image, above, because there are a couple with similar names.

We are already having fun, and collaboration is often the key to success!

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Which DNA Test Should I Buy? And Why?

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Which DNA test should I buy, and why?

I receive questions like this often. As a reminder, I don’t take private clients anymore, which means I don’t provide this type of individual consulting or advice. However, I’m doing the next best thing! In this article, I’m sharing the step-by-step process that I utilize to evaluate these questions so you can use the process too.

It’s important to know what questions to ask and how to evaluate each situation to arrive at the best answer for each person.

Here’s the question I received from someone I’ll call John. I’ve modified the wording slightly and changed the names for privacy.

I’m a male, and my mother was born in Charleston, SC. My maternal grandmother’s maiden name was Jones and a paternal surname was Davis. The family was supposed to have been Black, Dutch, Pennsylvania Dutch, and Scots-Irish…only once was I told I was 3/16 Indian, with Davis being 3/4 and Jones being full Indian.

Do I have enough reasonable information to buy a test, and which one?

Please note that it’s common for questions to arrive without all the information you need to provide a sound answer – so it’s up to you to ask those questions and obtain clarification.

Multiple Questions

There are actually multiple questions here, so let me parse this a bit.

  1. John never mentioned what his testing goal was.
  2. He also never exactly said how the paternal line of Davis was connected, so I’ve made an assumption. For educational purposes, it doesn’t matter because we’re going to walk through the evaluation process, which is the same regardless.
  3. John did not include a tree or a link to a tree, so I created a rudimentary tree to sort through this. I need the visuals and normally just sketch it out on paper quickly.
  4. Does John have enough information to purchase a test?
  5. If so, which test?

There is no “one size fits all” answer, so let’s discuss these one by one.

Easy Answers First

The answer to #4 is easy.

Anyone with any amount of information can purchase a DNA test. Adoptees do it all the time, and they have no prior information.

So, yes, John can purchase a test.

The more difficult question is which test, because that answer depends on John’s goals and whether he’s just looking for some quick information or really wants to delve into genealogy and learn. Neither approach is wrong.

Many people think they want a quick answer –  and then quickly figure out that they really want to know much more about their ancestors.

I wrote an article titled DNA Results – First Glances at Ethnicity and Matching for new testers, here.

Goals

Based on what John said, I’m going to presume his goals are probably:

  • To prove or disprove the family oral history of Black, Dutch, Pennsylvania Dutch (which is actually German,) Scots-Irish, and potentially Native American.
  • John didn’t mention actual genealogy, which would include DNA matches and trees, so we will count that as something John is interested in secondarily. However, he may need genealogy records to reach his primary goal.

If you’re thinking, “The process of answering this seemingly easy question is more complex than I thought,” you’d be right.

Ethnicity in General

It sounds like John is interested in ethnicity testing. Lots of people think that “the answer” will be found there – and sometimes they are right. Often not so much. It depends.

The great news is that John really doesn’t need any information at all to take an autosomal DNA test, and it doesn’t matter if the test-taker is male or female.

To calculate each tester’s ethnicity, every testing company compiles their own reference populations, and John will receive different results at each of the major companies. Each company updates their ethnicity results from time to time as well, and they will change.

Additionally, each company provides different tools for their customers.

The ethnicity results at different companies generally won’t match each other exactly, and sometimes the populations look quite different.

Normally, DNA from a specific ancestor can be found for at least 5 or 6 generations. Of course, that means their DNA, along with the DNA from all of your other ancestors is essentially combined in a communal genetic “pot” of your chromosomes, and the DNA testing company needs to sort it out and analyze your DNA for ethnicity.

DNA descended from ancestors, and their populations, further back in people’s trees may not be discerned at all using autosomal DNA tests.

A much more specific “ethnicity” can be obtained for both the Y-DNA line, which is a direct patrilineal line for men (blue arrow,) and the mitochondrial DNA line (pink arrows,) which is a direct matrilineal line for everyone, using those specific tests.

We will discuss both of those tests after we talk about the autosomal tests available from the four major genealogy DNA testing companies. All of these tools can and should be used together.

Let’s Start with Native American

Let’s evaluate the information that John provided.

John was told that he “was 3/16 Indian, with Davis being 3/4 and Jones being full Indian.”

We need to evaluate this part of his question slightly differently.

I discussed this in the article, Ancestral DNA Percentages – How Much of Them is in You?

First, we need to convert generations to 16ths.

You have two ancestors in your parent’s generation, four in your grandparents, and so forth. You have 16 great-great-grandparents. So, if John was 3/16th Native, then three of his great-great-grandparents would have been fully Native, or an equivalent percentage. In other words, six ancestors in that generation could have been half-Native. Based on what John said, they would have come from his mother’s side of the tree. John is fortunate to have that much information to work with.

He told us enough about his tree that we can evaluate the statement that he might be 3/16ths Native.

Here’s the tree I quickly assembled in a spreadsheet based on John’s information.

His father, at left, is not part of the equation based on the information John provided.

On his mother’s side, John said that Grandfather Davis is supposed to be three-quarters Native, which translates to 12/16ths. Please note that it would be extremely beneficial to find a Y-DNA tester from his Davis line, like one of his mother’s brothers, for example.

John said that his Grandmother Jones is supposed to be 100% Native, so 16/16ths.

Added together, those sum to 28/32, which reduces down to 14/16th or 7/8th for John’s mother.

John would have received half of his autosomal DNA from his mother and half from his non-Native father. That means that if John’s father is 100% non-Native, John would be half of 14/16ths or 7/16ths, so just shy of half Native.

Of course, we know that we don’t always receive exactly 50% of each of our ancestors’ DNA (except for our parents,) but we would expect to see something in the ballpark of 40-45% Native for John if his grandmother was 100% Native and his grandfather was 75%.

Using simple logic here, for John’s grandmother to be 100% Native, she would almost assuredly have been a registered tribal member, and the same if his grandfather was 75% Native. I would think that information would be readily available and well-known to the family – so I doubt that this percentage is accurate. It would be easy to check, though, on various census records during their lifetimes where they would likely have been recorded as “Indian.” They might have been in the special “Indian Census” taken and might be living on a reservation.

It should also be relatively easy to find their parents since all family members were listed every ten years in the US beginning with the 1850 census.

The simple answer is that if John’s grandparents had as much Native as reported, he would be more than 3/16th – so both of these factoids cannot simultaneously be accurate. But that does NOT mean neither is accurate.

John could be 7/8th or 40ish%, 3/16th or 18ish%, or some other percentage. Sometimes, where there is smoke, there is fire. And that seems to be the quandary John is seeking to resolve.

Would  Ethnicity/Population Tests Show This Much Native?

Any of the four major testing companies would show Native for someone whose percentage would be in the 40% or 18% ballpark.

The easiest ethnicities to tell apart from one another are continental-level populations. John also stated that he thinks he may also have Black ancestry, plus Dutch, Pennsylvania Dutch (German), and Scots-Irish. It’s certainly possible to verify that using genealogy, but what can DNA testing alone tell us?

How far back can we expect to find ethnicities descending from particular ancestors?

In this table, you can see at each generation how many ancestors you have in that generation, plus the percentage of DNA, on average, you would inherit from each ancestor.

All of the major DNA testing companies can potentially pick up small trace percentages, but they don’t always. Sometimes one company does, and another doesn’t. So, if John has one sixth-generation Native American ancestor, he would carry about 1.56% Native DNA, if any.

  • Sometimes a specific ethnicity is not found because, thanks to random recombination, you didn’t inherit any of that DNA from those ancestors. This is why testing your parents, grandparents, aunts, uncles, and siblings can be very important. They share your same ancestors and may have inherited DNA that you didn’t that’s very relevant to your search.
  • Sometimes it’s not found because the reference populations and algorithms at that testing company aren’t able to detect that population or identify it accurately, especially at trace levels. Every DNA testing company establishes their own reference populations and writes internal, proprietary ethnicity analysis algorithms.
  • Sometimes it’s not found because your ancestor wasn’t Native or from that specific population.
  • Sometimes it’s there, but your population is called something you don’t expect.

For example, you may find Scandinavian when your ancestor was from England or Ireland. The Vikings raided the British Isles, so while some small amount of Scandinavian is not what you expect, that doesn’t mean it‘s wrong. However, if all of your family is from England, it’s not reasonable to have entirely Scandinavian ethnicity results.

It’s also less likely as each generation passes by that the information about their origins gets handed down accurately to following generations. Most non-genealogists don’t know the names of their great-grandparents, let alone where their ancestors were from.

Using a 25-year average generation length, by the 4th generation, shown in the chart above, you have 16 ancestors who lived approximately 100 years before your parents were born, so someplace in the mid-1800s. It’s unlikely for oral history from that time to survive intact. It’s even less likely from a century years earlier, where in the 7th generation, you have 128 total ancestors.

The best way to validate the accuracy of your ethnicity estimates is by researching your genealogy. Of course, you need to take an ethnicity test, or two, in order to have results to validate.

Ethnicity has a lot more to offer than just percentages.

Best Autosomal Tests for Native Ethnicity

Based on my experience with people who have confirmed Native ancestry, the two best tests to detect Native American ethnicity, especially in smaller percentages, are both FamilyTreeDNA and 23andMe.

Click images to enlarge

In addition to percentages, both 23andMe and FamilyTreeDNA provide chromosome painting for ethnicity, along with segment information in download files. In other words, they literally paint your ethnicity results on your chromosomes.

They then provide you with a file with the “addresses” of those ethnicities on your chromosomes, which means you can figure out which ancestors contributed those ethnicity segments.

The person in the example above, a tester at FamilyTreeDNA, is highly admixed with ancestors from European regions, African regions and Native people from South America.

Trace amounts of Native American with a majority of European heritage would appear more like this.

You can use this information to paint your chromosome segments at DNAPainter, along with your matching segments to other testers where you can identify your common ancestors. This is why providing trees is critically important – DNA plus ancestor identification with our matches is how we confirm our ancestry.

This combination allows you to identify which Native (or another ethnicity) segments descended from which ancestors. I was able to determine which ancestor provided that pink Native American segment on chromosome 1 on my mother’s side.

I’ve provided instructions for painting ethnicity segments to identify their origins in specific ancestors, here.

Autosomal and Genealogy

You may have noticed that we’ve now drifted into the genealogy realm of autosomal DNA testing. Ethnicity is nice, but if you want to know who those segments came from, you’ll need:

  • Autosomal test matching to other people
  • To identify your common ancestor with as many matches as you can
  • To match at a company who provides you with segment information for each match
  • To work with DNAPainter, which is very easy

The great news is that you can do all of that using the autosomal tests you took for ethnicity, except at Ancestry who does not provide segment information.

Best Autosomal Test for Matching Other Testers

The best autosomal test for matching may be different for everyone. Let’s look at some of the differentiators and considerations.

If you’re basing a testing recommendation solely on database size, which will probably correlate to more matches, then the DNA testing vendors fall into this order:

If you’re basing that recommendation on the BEST, generally meaning the closest matches for you, there’s no way of knowing ahead of time. At each of the four DNA testing companies, I have very good matches who have not tested elsewhere. If I weren’t in all four databases, I would have missed many valuable matches.

If you’re basing that recommendation on which vendor began testing earliest, meaning they have many tests from people who are now deceased, so you won’t find their autosomal tests in other databases that don’t accept uploads, the recommended testing company order would be:

If you’re basing that recommendation on matches to people who live in other countries, the order would be:

Ancestry and 23andMe are very distant third/fourth because they did not sell widely outside the US initially and still don’t sell in as many countries as the others, meaning their testers’ geography is more limited. However, Ancestry is also prevalent in the UK.

If you’re basing that recommendation on segment information and advanced tools that allow you to triangulate and confirm your genetic link to specific ancestors, the order would be:

Ancestry does NOT provide any segment information.

If you’re basing that recommendation on unique tools provided by each vendor, every vendor has something very beneficial that the others don’t.

In other words, there’s really no clear-cut answer for which single autosomal DNA test to order. The real answer is to be sure you’re fishing in all the ponds. The fish are not the same. Unique people test at each of those companies daily who will never be found in the other databases.

Test at or upload your DNA to all four DNA testing companies, plus GEDmatch. Step-by-step instructions for downloading your raw data file and uploading it to the DNA testing companies who accept uploads can be found, here.

Test or Upload

Not all testing companies accept uploads of raw autosomal DNA data files from other companies. The good news is that some do, and it’s free to upload and receive matches.

Two major DNA testing companies DO NOT accept uploads from other companies. In other words, you have to test at that company:

Two testing companies DO accept uploads from the other three companies. Uploads and matching are free, and advanced features can be unlocked very cost effectively.

  • FamilyTreeDNA – free matching and $19 unlock for advanced features
  • MyHeritage – free matching and $29 unlock.for advanced features

I recommend testing at both 23andMe and Ancestry and uploading one of those files to both FamilyTreeDNA and MyHeritage, then purchasing the respective unlocks.

GEDmatch

GEDmatch is a third-party matching site, not a DNA testing company. Consider uploading to GEDmatch because you may find matches from Ancestry who have uploaded to GEDmatch, giving you access to matching segment information.

Other Types of DNA

John provided additional information that may prove to be VERY useful. Both Y-DNA and mitochondrial DNA can be tested as well and may prove to be more useful than autosomal to positively identify the origins of those two specific lines.

Let’s assume that John takes an autosomal test and discovers that indeed, the 3/16th Native estimate was close. 3/16th equates to about 18% Native which would mean that three of his 16 great-great-grandparents were Native.

John told us that his Grandmother Jones was supposed to be 100% Native.

At the great-great-grandparent level, John has 16 ancestors, so eight on his mother’s side, four from maternal grandmother Jones and four from his maternal grandfather Davis.

John carries the mitochondrial DNA of his mother (red boxes and arrows,) and her mother, through a direct line of females back in time. John also carries the Y-DNA of his father (dark blue box, at left above, and blue arrows below.)

Unlike autosomal DNA which is admixed in every generation, mitochondrial DNA (red arrows) is inherited from that direct matrilineal line ONLY and never combines with the DNA of the father. Mothers give their mitochondrial DNA to both sexes of their children, but men never contribute their mitochondrial DNA to offspring. Everyone has their mother’s mitochondrial DNA.

Because it never recombines with DNA from the father, so is never “watered down,” we can “see” much further back in time, even though we can’t yet identify those ancestors.

However, more importantly, in this situation, John can test his own mitochondrial DNA that he inherited from his mother, who inherited it from her mother, to view her direct matrilineal line.

John’s mitochondrial DNA haplogroup that will be assigned during testing tells us unquestionably whether or not his direct matrilineal ancestor was Native on her mother’s line, or not. If not, it may well tell us where that specific line originated.

You can view the countries around the world where Y-DNA haplogroups are found, here, and mitochondrial haplogroups, here.

If John’s mitochondrial DNA haplogroup is Native, that confirms that one specific line is Native. If he can find other testers in his various lines to test either their Y-DNA or mitochondrial DNA, John can determine if other ancestors were Native too. If not, those tests will reveal the origins of that line, separate from the rest of his genealogical lines.

Although John didn’t mention his father’s line, if he takes a Y-DNA test, especially at the Big Y-700 level, that will also reveal the origins of his direct paternal line. Y-DNA doesn’t combine with the other parent’s DNA either, so it reaches far back in time too.

Y-DNA and mitochondrial DNA tests are laser-focused on one line each, and only one line. You don’t have to try to sort it out of the ethnicity “pot,” wondering which ancestor was or was not Native.

My Recommendation

When putting together a testing strategy, I recommend taking advantage of free uploads and inexpensive unlocks when possible.

  • To confirm Native American ancestry via ethnicity testing, I recommend testing at 23andMe and uploading to FamilyTreeDNA, then purchasing the $19 unlock. The free upload and $19 unlock are less expensive than testing there directly.
  • For matching, I recommend testing at Ancestry and uploading to MyHeritage, then unlocking the MyHeritage advanced features for $29, which is less expensive than retesting. Ancestry does not provide segment information, but MyHeritage (and the others) do.

At this point, John will have taken two DNA tests, but is now in all four databases, plus GEDmatch if he uploads there.

  • For genealogy research on John’s lines to determine whether or not his mother’s lines were Native, I recommend an Ancestry and a MyHeritage records subscription, plus using WikiTree, which is free.
  • To determine if John’s mother’s direct matrilineal female line was Native, I recommend that John order the mitochondrial DNA test at FamilyTreeDNA.
  • When ordering multiple tests, or uploading at FamilyTreeDNA, be sure to upload/order all of one person’s tests on the same DNA kit so that those results can be used in combination with each other.

Both males and females can take autosomal and mitochondrial DNA tests.

  • To discover what he doesn’t know about his direct paternal, meaning John’s surname line – I recommend the Big Y-700 test at FamilyTreeDNA.

Only males can take a Y-DNA test, so women would need to ask their father, brother, or paternal uncle, for example, to test their direct paternal line.

  • If John can find a male Davis from his mother’s line, I recommend that he purchase the Big Y-700 test at FamilyTreeDNA for that person, or check to see if someone from his Davis line may have already tested by viewing the Davis DNA Project. Like with mitochondrial DNA, the Y-DNA haplogroup will tell John the origins of his direct Davis male ancestor – plus matching of course. He will be able to determine if they were Native, and if not, discover the origins of the Davis line.
  • For assigning segments to ancestors and triangulating to confirm descent from a common ancestor, I recommend 23andMe, MyHeritage, FamilyTreeDNA and GEDmatch, paired with DNAPainter as a tool.

Shopping and Research List

Here are the tests and links recommended above:

More Than He Asked

I realize this answer is way more than John expected or even knew to ask. That’s because there is often no “one” or “one best” answer. There are many ways to approach the question after the goal is defined, and the first “answer” received may be a bit out of context.

For example, let’s say John has 2% Native ancestry and took a test at a vendor who didn’t detect it. John would believe he had none. But a different vendor might find that 2%. If it’s on his mother’s direct matrilineal line, mitochondrial DNA testing will confirm, or refute Native, beyond any doubt, regardless of autosomal ethnicity results – but only for that specific ancestral line.

Autosomal DNA can suggest Native across all your DNA, but Y-DNA and mitochondrial DNA confirm it for each individual ancestor.

Even when autosomal testing does NOT show Native American, or African, for example, it’s certainly possible that it’s just too far back in time or has not been passed down during random recombination, but either Y-DNA or mitochondrial DNA will unquestionably confirm (or refute) the ancestry in question if the right person is tested.

This is exactly why I attempt to find a cousin who descends appropriately from every ancestor and provide testing scholarships. It’s important to obtain Y-DNA and mitochondrial DNA information for each ancestor.

Which Test Should I Order?

What steps will help you decide which test or tests to take?

  1. Define your testing goal.
  2. Determine if your Y-DNA or mitochondrial DNA will help answer the question.
  3. Determine if you need to find ancestors another generation or two back in time to get the most benefit from DNA testing. In our example, if John discovered that both of his grandparents were enrolled tribal members, that’s huge, and the tribe might have additional information about his family.
  4. Subscribe to Ancestry and MyHeritage records collections as appropriate to perform genealogical research. Additional information not only provides context for your family, it also provides you with the ability to confirm or better understand your ethnicity results.
  5. Extend your tree so that you can obtain the best results from the three vendors who support trees; Ancestry, FamilyTreeDNA, and MyHeritage. All three use trees combined with DNA tests to provide you with additional information.
  6. Order 23andMe and Ancestry autosomal DNA tests.
  7. Either test at or upload one of those tests to MyHeritage, FamilyTreeDNA, and GEDmatch.
  8. If a male, order the Big Y-700 DNA test. Or, find a male from your ancestral line who has taken or will take that test. I always offer a testing scholarship and, of course, share the exciting results!
  9. Order a mitochondrial DNA test for yourself and for appropriately descended family members to represent other ancestors. Remember that your father (and his siblings) all carry your paternal grandmother’s mitochondrial DNA. That’s often a good place to start after testing your own DNA.
  10. If your parents or grandparents are alive, or aunts and uncles, test their autosomal DNA too. They are (at least) one generation closer to your ancestors than you are and will carry more of your ancestors’ DNA.
  11. Your siblings will carry some of your ancestors’ DNA that you do not, so test them too if both of your parents aren’t available for testing.

Enjoy!!!

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Comparing DNA Results – Different Tests at the Same Testing Company

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Several people have asked about different tests at the same DNA testing company. They wondered if matching is affected, meaning whether your matches are different if you have two different tests at the same company. Specifically, they asked if you are better off purchasing a test AT a DNA testing vendor that allows uploads, rather than uploading a test from a different vendor. Does it make a difference to the tester or their matches? Do they have the same matches?

These are great questions, and the answer isn’t conclusive. It varies based on several factors.

Having multiple tests at the same DNA testing company can occur in three ways:

  • The same person tests twice at the same DNA testing company.
  • The same person tests once at the DNA testing company and uploads a test from a different testing company. Only two of the primary four DNA testing companies accept uploads from other vendors – FamilyTreeDNA and MyHeritage.
  • The same person uploads two different files from other DNA testing companies to the DNA testing company in question. For example, the DNA company could be FamilyTreeDNA and the two uploaded DNA files could be from either MyHeritage, 23andMe or Ancestry.

All DNA testing companies allow users to download their raw DNA data files. This enables the tester to upload their DNA file to the vendors who accept uploaded files. Both FamilyTreeDNA and MyHeritage provide matching for free, but advanced tools require a small unlock fee of $19 and $29, respectively.

Testing Company Accepts Uploads from Other Companies Download Upload Instructions
23andMe No Instructions here
Ancestry No Instructions here
FamilyTreeDNA Yes, some Instructions here
MyHeritage Yes, some Instructions here

I wrote about developing a DNA testing and transfer/upload strategy, here, and about which companies accept which tests, here.

Not all DNA files are created equal. Therefore, not all files from vendors are compatible with other vendors for various reasons.

Multiple Tests at the Same DNA Testing Company

I have at least two tests at each of the four major vendors. I did this for research purposes, meaning to write articles to share with you.

If you actually test twice at a vendor, meaning purchase two separate tests and take them yourself, you will have two test results at that testing company. At some companies, specifically 23andMe, if you purchase a new test through their “upgrade” procedure, you won’t have two tests, just the newer one.

However, if you’re testing at the DNA testing company, and also uploading, I generally don’t recommend more than one test at each vendor. All it really does is clog up people’s match lists with no or little additional benefit. At 23andMe, with their restrictions on the size of your match list, if everyone had two tests, the effective match limit would be half of their stated limit of about 1500 matches for earlier testers and about 5000 for current testers with subscriptions.

So, in essence, I’m telling you to “do as I say, not as I do.” We all have better things to do with our money rather pay for the same test twice. If you haven’t tested your Y-DNA or mitochondrial DNA, that’s much more beneficial than two autosomal tests at one vendor.

Chips and Chip Evolution

Before we begin the side-by-side comparison, let’s briefly discuss DNA testing chips and how they work.

Each DNA testing company purchases DNA processing equipment. Illumina is the big dog in this arena. Illumina defines the capacity and structure of each chip. In part, how the testing companies use that capacity, or space on each chip, is up to each company. This means that the different testing companies test many of the same autosomal DNA SNP locations, but not all of the same locations.

Furthermore, the individual testing companies can specify a number of “other” locations to be included on their chip, up to the chip maximum size limit. The testing companies who offer Y-DNA or mitochondrial DNA haplogroups from autosomal tests use part of their chip array space for selected known haplogroup-defining SNP locations. This does NOT mean that Y-DNA or mitochondrial DNA is autosomal, just that the testing company used part of their chip array space to target these SNPs in your genome. Of course, for your most refined haplogroup and Y-DNA or mitochondrial DNA matching, you have to take those specific tests at FamilyTreeDNA .

This means that each testing company includes and reports many of the same, but also some different SNP locations when they scan your DNA.

In the lab, after your DNA is extracted from either your saliva or the cheek swab, it’s placed on this array chip which is then placed in the processing equipment.

There are several steps in processing your DNA. Each DNA location specified on the chip is scanned and read multiple times, and the results are recorded. The final output is the raw DNA results file that you see if/when you download your raw DNA file.

Here’s an example from my file. The RSID is the reference SNP cluster ID which is the naming convention used for specific SNPs. It’s not relevant to you, but it is to the lab, along with the chromosome number and position, which is in essence the address on the chromosome.

In the Result column, your file reports one nucleotide (T, A, C or G) that you inherited from each parent at each tested position. They are not listed in “parent order” because your DNA is not organized in that fashion. There’s no way for the lab to know which nucleotide came from which parent, unless they are the same, of course. You can read about nucleotides, here.

When you upload your raw DNA file to a different DNA testing company (vendor), they have to work with a file that isn’t entirely compatible with the files they generate, or the other files uploaded from other DNA testing companies.

In addition to dealing with different file formats and contents from multiple DNA vendors, companies change their own chips and file structure from time to time. In some cases, it’s a forced change by the chip manufacturer. Other times, the vendors want to include different locations or make improvements. For example, with 23andMe’s focus on health, they probably add new medically related SNP locations regularly. Regardless of why, some DNA files include locations not included in other files and are not 100% compatible.

Looking at the first few entries in my example file above, let’s say that the testing vendor included the first ten positions, but an uploaded file from another company did not. Or perhaps the chip changed, and a different version of the company’s own file contains different positions.

DNA testing companies have to “fill in the blanks” for compatibility, and they do this using a technique called imputation. Illumina forced their customers to adopt imputation in 2017 when they dropped the capacity of their chip. I was initially quite skeptical, but imputation has worked surprisingly well. Some of the matching differences you will see when comparing the results of two different DNA files is a result of imputation.

I wrote about imputation in an early article here. Please note the companies have fixed many issues with imputation and improved matching greatly, but the concepts and imputation processes still apply. The downloaded raw data files are your results BEFORE imputation, meaning that it’s up to any company where you upload to process your raw file in the same way they would process a file that they generated. A lot goes on behind the scenes when you upload a file to a DNA testing company.

At both 23andMe and Ancestry, you know that all of your matches tested there, meaning they did not upload a file from another testing company. You don’t know and can’t tell what chip was utilized when your matches tested. The only way to determine a chip testing version, aside from knowing the date or remembering the chip version from when you tested, is to look at the beginning of the raw data download file, although not all files contain that information.

Ok, now that you understand the landscape, let’s look at my results at each company.

23andMe

I tested twice at 23andMe on two different chip versions, V3 and V4, which tested some different locations of my DNA. Neither of these chips is the current version. I originally tested twice to evaluate the differences between the two test versions which you can read about, here.

23andMe named their ethnicity results Ancestry Composition.

They last updated my V3 test’s Ancestry Composition results on July 28, 2021.

The percentages are shown at left, and the country locations are highlighted at right for my 23andMe V3 test.

Click to enlarge any graphic

The 23andMe V4 test was also updated for the last time on July 28, 2021.

The ethnicity results differ substantially between the two chip versions, even though they were both updated on the same date.

In October of 2020, in an effort to “encourage” their customers to pay for a new test on their V5 chip, 23andMe announced that there would be no ethnicity updates on older tests. So, I really don’t know for sure when my tests were actually updated. Just note how different the results are. It’s also worth mentioning that 23andMe does not show trace amounts on their map, so even though my Indigenous American results were found, they aren’t displayed on the map.

Indigenous is, however, shown in yellow on their DNA Chromosome Painting.

No other testing company restricts updates, penalizing their customers who purchased earlier versions of tests.

Matches at 23andMe

23andMe limits your matches to about 1500 unless you have purchased the current test, including health AND pay for an annual $69 subscription which buys you about 5000 matches. I have not purchased this test.

Your number of actual matches displayed/retained is also affected by how many people you have communicated with, or at least initiated communications with. 23andMe does not roll those people off of your match list.

I have 1803 matches on both of my tests, meaning I’ve reached out to about 300 people who would have otherwise been removed from my match list. 23andMe retains your highest matches, deleting lower matches after you reach the maximum match threshold.

I’ve randomly evaluated several of the same matches at each vendor, at least five maternal and five paternal, separated by a blank row. I wanted to determine whether they match me on the same number of centimorgans, meaning the same amount of DNA, on both tests, and the same number of segments.

Match 23and Me V3 23and Me V4
Patricia 292 cM – 12 segments Same as V3
Joe 148 cM, 8 segments Same
Emily 73 cM, 4 segs 72 cM, 4 seg
Roland 27 cM, 1 seg Same
Ian 62 cM, 4 seg Same
Stacy 469 cM, 16 segments 482 cM, 16 segments
Harold 134 cM, 6 segments Same
Dean 69 cM, 3 seg Same
Carl 95 cM, 4 seg Same
Debbie 83 cM, 4 seg 84 cM, 4 seg

As you can see, the matches are either exact or xclose.

Please note that bolded matches are also found at another company. I will include a summary table at the end comparing the same match across multiple vendors.

23and Me Summary

The 23andMe V3 and V4 match results are very close. Since the match limit is the same, and the results are so close between tests, they are essentially identical in terms of matching.

The ethnicity results are similar, but the V4 test reflects a broader region. Italian baffles me in both versions.

Ethnicity should never be taken at face value at any DNA testing company, especially with smaller percentages which could be noise or a combination of other regions which just happens to resemble Italy, in my case.

I don’t know what type of comparison the current chip would yield since I suspect it has more medical and less genealogical SNPs on board.

Reprocessing Tests

This is probably a good place to note that it’s very expensive for any company to update their customer’s ethnicity results because every single customer’s DNA results file must be completely rerun. Note that this does not mean their DNA itself is retested. The output raw data file is reprocessed using a new algorithm.

Rerunning means reprocessing that specific portion of every test, meaning the vendors must rent “time in the cloud.” We are talking millions of dollars for each run. I don’t know how much it costs per test, but think about the expense if it takes $1 to rerun each test in the vendor’s database. Ancestry has more than 20 million tests.

While we, as consumers, are always chomping at the bit for new and better ethnicity results – the testing companies need to be sure it really is “better,” not just different before they invest the money to reprocess and update results.

This is probably why 23andMe decided to cease updating older kits. The newer tests require a subscription which is recurring revenue.

The same is true when DNA testing companies need to rematch their entire user base. This happens when the criteria for matching changes. For example, Ancestry purged a large number of matches for all of their customers back in 2020. While match algorithm changes necessitate rematching, with associated costs, this change also provided Ancestry with the huge benefit of eliminating approximately half of their customer’s matches. This freed up storage space, either physically in their data center or space rented in the cloud, representing substantial cost-savings.

How long can a DNA testing company reasonably be expected to continue investing in a product which never generates additional revenue but for which the maintenance and reinvestment costs never end?

Ancestry and MyHeritage both hope to offset the expenses of maintaining their customer’s DNA tests and providing free updates by selling subscriptions to their record services. 23andMe wants you to purchase a new test and a yearly subscription. FamilyTreeDNA wants you to purchase a Big Y-DNA and mitochondrial DNA test.

OK, now let’s look at my matches at Ancestry.

Ancestry

I’ve taken two Ancestry tests, V1 and V2. There were some differences, which I wrote about here and here. V2 is no longer the current chip.

Except for 23andMe who wants their customers to purchase their most current test, the other companies no longer routinely announce new chip versions. They just go about their business. The only way you know that a vendor actually changed something is when the other companies who accept uploads suddenly encounter an issue with file formats. It always takes a few weeks to sort that out.

My Ancestry V1 test’s ethnicity results don’t show my Native American ethnicity.

Ancestry results were updated in June 2022

However, my V2 results do include Native American ethnicity.

Matches at Ancestry

I have many more matches on my V1 test at Ancestry because I took steps to preserve my smaller matches when Ancestry initiated its massive purge in 2020. I wrote about that here and here.

Ancestry’s SideView breaks matches down into maternal, paternal, and unassigned based on your side selection. You tell Ancestry which side is which. You may be able to determine which “side” is maternal or paternal either by your ethnicity or shared matches. While SideView is not always accurate, it’s a good place to begin.

Match Category Ancestry V1 Test Ancestry V2 Test
Maternal 15,587 15,116
Paternal 42,247 41,870
Both 2 2
Unassigned 48,999 4,127
Total 106,835 61,115

Ancestry either displays all your matches or your matches by side, which I used to compile the table above. I suspect that Ancestry is not assigning any of the smaller preserved matches to “sides” based on the numbers above.

Ancestry implemented a process called Timber that removes DNA that they feel is “too matchy,” meaning you match enough people in this region that they think it’s a pileup region for you personally, and therefore not useful. In some cases, enough DNA is removed causing that person to no longer be considered a match because they fall beneath the match threshold. I am not a fan of Timber.

Your match amount shown is AFTER Timber has removed those segments. Unweighted shared DNA is your pre-Timber match amount.

You can view the Unweighted shared DNA by clicking on the amount of shared DNA on your match list.

You can read Ancestry’s Matching White Paper, here.

Let’s take a look at my matches. I’ve listed both weighted and unweighted where they are different.

Match Ancestry V1 Ancestry V2
Michael 755 cM, 35 seg 737 cM, 33 seg
Edward 66 cM, 4 seg (unweighted 86 cM) 65 cM, 4 seg (unweighted 86 cM)
Tom 59 cM, 3 seg (unweighted 63) Same
Jonathon 43 cM, 4 seg, (unweighted 52 cM) Same
Matthew 20 cM, 2 seg (unweighted 35 cM) Same
Harold 132 cM, 7 seg 135 cM, 6 seg
Dean 67 cM, 4 seg (unweighted 78 cM) 66 cM, 4 seg (unweighted 78 cM)
Debbie 93 cM, 5 seg Same
Valli 142 cM, 3 seg Same
Jared 20 cM, 1 seg (unweighted 22 cM) Same

Timber only removes DNA when the match is under 90 cM. Almost every match under 90 cM has some DNA removed.

Ancestry Summary

The results of the two Ancestry tests are very close.

In some circumstances, no DNA is removed by Timber, so the unweighted is the same as the weighted. However, in other cases, a significant amount is removed. 15 cM of Matthew’s 35 cM was removed by Timber, reducing his total to 20 cM.

Remember that Ancestry does not show shared matches unless they are greater than 20 cM, which is different than any other DNA testing company.

At one point, Ancestry was selling a health test that was also a genealogy test. That test utilized a different chip that is not accepted for uploads by other vendors. The results of that test might well be different that the “normal” Ancestry tests focused on genealogy. The Ancestry health test is no longer offered.

Companies that Accept Uploads

DNA testing companies that accept uploaded DNA files from other DNA testing companies need to process the uploaded file, just like a file that is generated in their own lab. Of course, they must deal with the differences between uploaded files and their own file format. The processing includes imputation and formulates the uploaded file so that it works with the tools that they provide for their customers, including ethnicity (by whatever name they use) matching, family matching (bucketing), advanced matching, the match matrix, triangulation, AutoClusters, Theories of Family Relativity, and other advanced tools.

Of course, the testing company accepting uploads can only work with the DNA locations provided by the original DNA testing company in the uploaded file.

Matching and some additional tools are free to uploaders, but advanced tools require an inexpensive unlock.

FamilyTreeDNA

I took a test at FamilyTreeDNA, plus uploaded a copy of both of my Ancestry DNA files.

FamilyTreeDNA named their population (ethnicity) test myOrigins and the current version is V3. I wrote about the rollout and comparison in September of 2020, here.

My DNA test taken at FamilyTreeDNA, above, reveals Native American segments that match reference populations found both in North and South America and the Caribbean Islands.

At FamilyTreeDNA, my Ancestry V1 uploaded file results show Native American population matches only in North America.

Interestingly, my Ancestry V1 file processed AT Ancestry did not reveal Native American ancestry, but the same file uploaded to and processed at FamilyTreeDNA did show Native American results, reflecting the difference between the vendors’ internal algorithms and reference populations utilized.

My myOrigins results from my Ancestry V2 uploaded file at FamilyTreeDNA also include my North American Native American segments. The V2 test also showed Native American ethnicity at Ancestry, so clearly something changed in Ancestry’s algorithm, locations tested, and/or reference populations between V1 and V2.

Fortunately, FamilyTreeDNA provides both chromosome painting and a population download file so I can match those Native segments with my autosomal matches to identify which of my ancestors contributed those specific segments.

One of my Native segments is shown in pink on Chromosome1. My mother has a Native segment in exactly the same location, so I know that this segment originated with my mother’s ancestors.

I downloaded the myOrigins population segment file and painted my results at DNAPainter, along with the matches where I can identify our common ancestor. This allowed me to pinpoint the ancestral line that contributed this Native segment in my maternal line. You can read about using DNAPainter, here.

FamilyTreeDNA Matches

I have significantly more matches at FamilyTreeDNA on their test than on either of my Ancestry tests that I uploaded. However, nearly the same number are maternally or paternally assigned through Family Matching, with the remainder unassigned. You can read about Family Matching here.

Match Category FamilyTreeDNA Test Ancestry V1 at FamilyTreeDNA Ancestry V2 at FamilyTreeDNA
Paternal 3,479 3,572 3,422
Maternal 1,549 1,536 1,477
Both 3 3 3
All 8,154 6,397 6,579

Family matching, aka bucketing, automatically assigns my matches as maternal and paternal by linking known relatives to their place in my tree.

I completed the following match chart using my original test taken at FamilyTreeDNA, plus the same match at FamilyTreeDNA for both of my Ancestry tests.

In other words, Cheryl matched me at 467 cM on 21 segments on the original test taken at FamilyTreeDNA. She matched me on 473 cM and 21 segments on my Ancestry V1 test uploaded to FamilyTreeDNA and on 483 cM and 22 segments on the Ancestry V2 test uploaded to FamilyTreeDNA.

Match FamilyTreeDNA Ancestry V1 at FTDNA Ancestry V2 at FTDNA
Cheryl 467 cM, 21 seg 473 cM, 21 seg 483 cM, 22 seg
Patricia 195 cM, 11 seg 189 cM, 11 seg 188 cM, 11 seg
Tom 77 cM, 4 seg 71 cM, 4 seg 76 cM, 4 seg
Thomas 72 cM, 3 seg 71 cM, 3 seg 74 cM, 3 seg
Roland 29 cM, 1 seg 35 cM, 2 seg 35 cM, 2 seg
Rex 62 cM, 4 seg 55 cM, 3 seg 57 cM, 3 seg
Don 395 cM, 18 seg 362 cM, 15 seg 398 cM, 18 seg
Ian 64 cM, 4 seg 56 cM, 4 seg 64 cM, 4 seg
Stacy 490 cM, 18 seg 494 cM, 15 seg 489 cM, 14 seg
Harold 127 cM, 5 cM 133 cM, 6 seg 143 cM, 6 seg
Dean 81 cM, 4 seg 75 cM, 3 seg 83 cM, 4 seg
Carl 103 cM, 4 seg 101 cM, 4 seg 102 cM, 4 seg
Debbie 99 cM, 5 seg 97 cM, 5 seg 99 cM, 5 seg
David 373 cM, 16 seg 435 cM, 19 seg 417 cM, 18 seg
Amos 176 cM, 7 seg 177 cM. 8 seg 177 cM, 7 seg
Buster 387 cM, 15 seg 396 cM, 16 seg 402 cM, 17 seg
Charlene 461 cM, 21 seg 450 cM, 21 seg 448 cM, 20 seg
Carol 65 cM, 6 seg 64 cM, 6 seg 65 cM, 6 seg

I have tested many of my cousins at FamilyTreeDNA and encouraged others to test or upload. I’ve attempted to include enough people so that I can have common matches at least at one other DNA testing company for comparison.

FamilyTreeDNA Summary

The matches are relatively close, with a few being exact.

Interestingly, some of the segment counts are different. In most cases, this results from one segment being broken into multiple segments by one or more of the tests, but not always. In the couple that I checked, the entire segment seems to descend from the same ancestral couple, so the break is likely a result of not all of the same DNA locations being tested, plus the limits of imputation.

MyHeritage

I have two tests at MyHeritage. One taken at MyHeritage, and an uploaded file from FamilyTreeDNA.

MyHeritage displays both ethnicity results and Genetic Groups which maps groups of people that you match. I left the Genetic Groups setting at the highest confidence level. Shifting it to lower displays additional Genetic Groups, some of which overlap with or are within ethnicity regions.

My test taken at MyHeritage, above, shows several ethnicities and Genetic Groups, but no Native American.

My FamilyTreeDNA kit processed at MyHeritage shows the same ethnicity regions, one additional Genetic Group, plus Native American heritage in the Amazon which is rather surprising given that I don’t show Native in North American regions where I’m positive my Native ancestors lived.

MyHeritage Matching

At MyHeritage, I compared the results of the test I took with MyHeritage, and a test I uploaded from FamilyTreeDNA. Fewer than half of my matches can be assigned to a parent via shared matching.

Matches MyHeritage Test FamilyTreeDNA at MyHeritage
Paternal 4,422 6,501
Maternal 2,660 3,655
Total 13,233 16,147

I have rounded my matches at MyHeritage to the closest cM.

Match MyHeritage Test FamilyTreeDNA at MyHeritage
Michael 801 cM, 32 seg 823 cM, 31 segments
Cheryl 467 cM, 23 seg 477 cM, 23 seg
Roland No match 28 cM, 1 seg
Patty 156 cM, 9 seg 151 cM, 9 seg
Rex 43 cM, 4 seg 53 cM, 3 seg
Don 369 cM, 16 seg 382 cM, 17 seg
 
David 449 cM, 17 seg 460 cM, 17 seg
Charlene 454 cM, 23 seg 477 cM, 24 seg
Buster 408 cM, 15 seg 410 cM, 16 seg
Amos 183 cM, 8 seg Same
Carol 78 cM, 6 seg 87 cM, 7 seg

MyHeritage Summary

I was surprised to discover that Roland had no match with the MyHeritage test, but did with the FamilyTreeDNA test. I wonder if this is a searching or matching glitch, especially since both companies use the same chip. 28 cM in one segment is a reasonably large match, and even if it was divided in two, it would still be over the matching threshold. I know this is a valid match because Roland triangulates with me and several cousins, I’m positive of our common ancestor, and he also matches me at both FamilyTreeDNA and 23andMe.

Other than that, the matches are reasonably close, with one being exact.

Your Matches Aren’t Everyplace

I unsuccessfully searched for someone who was a match to me in all four databases. Ancestry does not permit match downloads, so I had to search manually. People don’t always use the same names in different databases.

Surprisingly, I was unable to find one match who is in all of the databases. Many people only suggest testing at Ancestry because they have the largest database, but if you look at the following comparison chart that I’ve created, you’ll see that 16 of 26 people, or 62% were not at Ancestry. Conversely, many people were at Ancestry and not elsewhere. I could not find five maternal and five paternal matches at Ancestry that I could identify as matches in another database. 40% were not elsewhere.

If you think for one minute that it doesn’t matter for genealogy if you’re in all four major databases, please reconsider. It surely does matter.

Every single vendor has matches that the others don’t. Substantial, important matches. I have found first and second-cousin matches in every database that weren’t elsewhere.

Many of the original testers have passed away and can’t test again. My mother can never test at either 23andMe or Ancestry, but she is at both FamilyTreeDNA and MyHeritage because I could upgrade her kit at FamilyTreeDNA after she died. I uploaded her to MyHeritage. Of course, because she is a generation closer to our ancestors, she has many valuable matches that I don’t.

Each vendor provides either an email address or a messaging platform for you to contact your matches. Don’t be discouraged if they don’t answer. Just today, I received a reply that was years in the making.

Genealogists hope for immediate gratification, but we are actually in this for the long game. Play it with every tool at your disposal.

The Answer

Does it matter if you test at a DNA testing company, or upload a file?

I know this was a very long answer to what my readers hoped was a simple yes or no question.

There is no consistent answer at either FamilyTreeDNA or MyHeritage, the two DNA testing companies that accept uploads. Be sure you’re in both databases. My closest two matches that I did not test were found at MyHeritage. Here’s a direct link to upload at MyHeritage.

Of the vendors, those two should be the closest to each other because they are both processed in the GenebyGene lab, but again, the actual chip version, when the test was originally taken, and each vendor’s internal processing will result in differences. Neither the original test at the DNA testing company nor the uploaded files have consistently higher or lower matches. Neither type of test or upload appears to be universally more or less accurate. Differences in either direction seem to occur on a match-by-match basis. Many are so close as to be virtually equivalent, with a few seemingly random exceptions. Of course, we always have to consider Timber.

If you upload, unlock the advanced features at both FamilyTreeDNA and MyHeritage.

If you upload to a DNA testing company, you may discover in the future that some features and functions will only be available to original testers.

Personally, if I had the option, I would test at the company directly simply because it eliminates or at least reduces the possibility of future incompatibilities – with the exception of 23andMe which has chosen to not provide consistent updates to older tests. I’m incredibly grateful I didn’t test my mother or now deceased family members at 23andMe, and only there. I would be heartsick, heartbroken, and furious.

Our DNA is an extremely valuable resource for our genealogy. It’s the gift that truly keeps on giving, day after day, even when other records don’t exist. Be sure you and your family members are in each database one way or another, and test your Y-DNA (for males) and mitochondrial DNA (for everyone) to have a complete arsenal at your disposal.

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What Is a Sibling Anyway? Full, Half, Three-Quarters, Step, Adopted, Donor-Conceived & Twins

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I’ve seen the term sibling used many different ways, sometimes incorrectly.

When referring to their own siblings, people usually use the term brother or sister, regardless of whether they are talking about a full, half or step-sibling. It’s a term of heart or description. It’s often genealogists who are focused on which type of sibling. As far as I’m concerned, my brother is my brother, regardless of which type of brother. But in terms of genetics, and genealogy, there’s a huge difference. How we feel about our sibling(s) and how we are biologically related are two different things.

Let’s cover the various types of siblingship and how to determine which type is which.

  • Full Siblings – Share both parents
  • Half-Siblings – Share only one parent
  • Three-Quarter Siblings – It’s complicated
  • Adopted Siblings
  • Donor-Conceived
  • Step-Siblings – Share no biological parent
  • Twins – Fraternal and Identical

Full Siblings

Full siblings share both parents and share approximately 50% of their DNA with each other.

You can tell if you are full siblings with a match in various ways.

  1. You share the same fairly close matches on both parents’ sides. For example, aunts or uncles or their descendants.

Why do I say close matches? You could share one parent and another more distant relative on the other parent’s side. Matching with close relatives like aunts, uncles or first cousins at the appropriate level is an excellent indicator unless your parents or grandparents are available for testing. If you are comparing to grandparents, be sure to confirm matches to BOTH grandparents on each side.

  1. Full siblings will share in the ballpark of 2600 cM, according to DNAPainter’s Shared cM Tool.

Keep in mind that you can share more or less DNA, hence the range. It’s also worth noting that some people who reported themselves as full siblings in the Shared cM project were probably half siblings and didn’t realize it.

  1. Full siblings will share a significant amount of fully identical regions (FIR) of DNA with each other, meaning they share DNA at the same DNA address from both parents, as illustrated above. Shared DNA with each other inherited from Mom and Dad are blocked in green. The fully identical regions, shared with both parents, are bracketed in purple. You can’t make this determination at FamilyTreeDNA, MyHeritage or Ancestry, but you can at both 23andMe and GEDmatch.

At GEDmatch, the large fully green areas in the chromosome browser “graphics and positions” display indicates full siblings, where DNA is shared from both parents at that location.

I wrote about the details of how to view fully identical regions (FIR) versus half identical regions (HIR) in the article, DNA: In Search of…Full and Half-Siblings.

  1. If your parents/grandparents have tested, you and your full sibling will both match both parents/grandparents. Yes, I know this sounds intuitive, but sometimes it’s easy to miss the obvious.

At FamilyTreeDNA, you can use the matrix tool to see who matches each other in a group of people that you can select. In this case, both siblings are compared to the father, but if the father isn’t available, a close paternal relative could substitute. Remember that all people who are 2nd cousins or closer will match.

  1. At Ancestry, full siblings will be identified as either “brother” or “sister,” while half-siblings do not indicate siblingship. Half-siblings are called “close family” and a range of possible relationships is given. Yes, Ancestry, is looking under the hood at FIR/HIR regions. I have never seen a full sibling misidentified as anything else at Ancestry. Unfortunately, Ancestry does not give customers access to their matching chromosome segment location data.
  2. Y-DNA of males who are full siblings will match but may have some slight differences. Y-DNA alone cannot prove a specific relationship, with very rare exceptions, but can easily disprove a relationship if two males do not match. Y-DNA should be used in conjunction with autosomal DNA for specific relationship prediction when Y-DNA matches.
  3. Y-DNA testing is available only through FamilyTreeDNA, but high-level haplogroup-only estimates are available through 23andMe. Widely divergent haplogroups, such as E versus R, can be considered a confirmed non-match. Different haplogroups within the same base haplogroup, such as R, but obtained from different vendors or different testing levels may still be a match if they test at the Big Y-700 level at FamilyTreeDNA.
  4. Mitochondrial DNA, inherited matrilineally from the mother, will match for full siblings (barring unusual mutations such as heteroplasmies) but cannot be used in relationship verification other than to confirm nonmatches. For both Y-DNA and mitochondrial DNA, it’s possible to have a lineage match that is not the result of a direct parental relationship.
  5. Mitochondrial DNA testing is available only through FamilyTreeDNA, but haplogroup-only estimates are included at 23andMe. Different base haplogroups such as H and J can be considered a non-match.
  6. A difference in ethnicity is NOT a reliable indicator of half versus full siblings.

Half-Siblings

Half-siblings share only one parent, but not both, and usually share about 25% of their DNA with each other.

You will share as much DNA with a half-sibling as you do some other close matches, so it’s not always possible for DNA testing companies to determine the exact relationship.

Referencing the MyHeritage cM Explainer tool, you can see that people who share 1700 cM of DNA could be related in several ways. I wrote about using the cM Explainer tool here.

Hints that you are only half-siblings include:

  1. At testing vendors, including Ancestry, a half-sibling will not be identified as a sibling but as another type of close match.
  2. If your parents or grandparents have tested, you will only match one parent or one set of grandparents or their descendants.
  3. You will not have shared matches on one parent’s side. If you know that specific, close relatives have tested on one parent’s side, and you don’t match them, but your other family members do, that’s a very big hint. Please note that you need more than one reference point, because it’s always possible that the other person has an unknown parentage situation.
  4. At 23andMe, you will not show fully identical regions (FIR).
  5. At GEDmatch, you will show only very minimal FIR.

Scattered, very small green FIR locations are normal based on random recombination. Long runs of green indicate that significant amounts of DNA was inherited from both parents. The example above is from half-siblings.

  1. At FamilyTreeDNA and 23andMe, most men who share a mother will also share an X chromosome match since men only inherit their X chromosome from their mother. However, it is possible for the mother to give one son her entire X chromosome from her father, and give the other son her entire X chromosome from her mother. Therefore, two men who do share a mother but don’t have an X chromosome match could still be siblings. The X is not an entirely reliable relationship predictor. However, if two men share an entire X chromosome match, it’s very likely that they are siblings on their mother’s side, or that their mothers are very close relatives.

Three-Quarter Siblings

This gets a little more complicated.

Three-quarter siblings occur when one parent is the same, and the other parents are siblings to each other.

Let’s use a real-life example.

A couple marries and has children. The mother dies, and the father marries the mother’s sister and has additional children. Those children are actually less than full siblings, but more than half-siblings.

Conversely, a woman has children by two brothers and those children are three-quarter siblings.

These were common situations in earlier times when a man needed a female companion to raise children and women needed a male companion to work on the farm. Neither one could perform both childcare and the chores necessary to earn a living in an agricultural society, and your deceased spouse’s family members were already people you knew. They already loved your children too.

Neither of these situations is historically unusual, but both are very difficult to determine using genetics alone, even in the current generation.

Neither X-DNA nor mitochondrial DNA will be helpful, and Y-DNA will generally not be either.

Unfortunately, three-quarter siblings’ autosomal DNA will fall in the range of both half and full siblings, although not at the bottom of the half-sibling range, nor at the top of the full sibling range – but that leaves a lot of middle ground.

I’ve found it almost impossible to prove this scenario without prior knowledge, and equally as impossible to determine which of multiple brothers is the father unless there is a very strong half-sibling match in addition.

The DNA-Sci blog discusses this phenomenon, but I can’t utilize comparison screenshots according to their terms of service.

Clearly, what we need are more known three-quarter siblings to submit data to be studied in order to (possibly) facilitate easier determination, probably based on the percentage frequency distribution of FIR/HIR segments. Regardless, it’s never going to be 100% without secondary genealogical information.

Three-quarter siblings aren’t very common today, but they do exist. If you suspect something of this nature, really need the answer, and have exhausted all other possibilities, I recommend engaging a very experienced genetic genealogist with experience in this type of situation. However, given the random nature of recombination in humans, we may never be able to confirm using any methodology, with one possible exception.

There’s one possibility using Y-DNA if the parents in question are two brothers. If one brother has a Y-DNA SNP mutation that the other does not have, and this can be verified by testing either the brothers who are father candidates or their other known sons via the Big Y-700 test – the father of the siblings could then be identified by this SNP mutation as well. Yes, it’s a long shot.

Three-quarter sibling situations are very challenging.

Step-siblings, on the other hand, are easy.

Step-Siblings

Step-siblings don’t share either parent, so their DNA will not match to each other unless their parents are somehow related to each other. Please note that this means either of their parents, not just the parents who marry each other.

One child’s parent marries the other child’s parent, resulting in a blended family. The children then become step-siblings to each other.

The terms step-sibling and half-sibling are often used interchangeably, and they are definitely NOT the same.

Adopted Siblings

Adopted siblings may not know they are adopted and believe, until DNA testing, that they are biological siblings.

Sometimes adopted siblings are either half-siblings or are otherwise related to each other but may not be related to either of their adoptive parents. Conversely, adopted siblings, one or both, may be related to one of their adoptive parents.

The same full and half-sibling relationship genetic clues apply to adopted siblings, as well as the tools and techniques in the In Search of Unknown Family series of articles.

Donor-Conceived Siblings

Donor-conceived siblings could be:

  • Half-siblings if the donor is the same father but a different mother.
  • Half-siblings if they share an egg donor but not a father.
  • Full siblings if they are full biological siblings to each other, meaning both donors are the same but not related to the woman into whom the fertilized egg was implanted, nor to her partner, their legal parents.
  • Not biologically related to each other or either legal parent.
  • Biologically related to one or both legal parents when a family member is either an egg or sperm donor.

Did I cover all of the possible scenarios? The essence is that we literally know nothing and should assume nothing.

I have known of situations where the brother (or brothers) of the father was the sperm donor, so the resulting child or children appear to be full or three-quarters siblings to each other. They are related to their legal father who is the mother’s partner. In other words, in this situation, the mother’s husband was infertile, and his brother(s) donated sperm resulting in multiple births. The children from this family who were conceived through different brothers and had very close (half-sibling) matches to their “uncles'” children were very confused until they spoke with their parents about their DNA results.

The same techniques to ascertain relationships would be used with donor-conceived situations. Additionally, if it appears that a biological relationship exists, but it’s not a full or half-sibling relationship, I recommend utilizing other techniques described in the In Search of Unknown Family series.

Twins or Multiple Birth Siblings

Two types of twin or multiple birth scenarios exist outside of assisted fertilization.

Fraternal twins – With fraternal or dizygotic twins, two eggs are fertilized independently by separate sperm. Just view this as one pregnancy with two siblings occupying the same space for the same 9 months of gestation. Fraternal twins can be male, female or one of each sex.

Fraternal twins are simply siblings that happen to gestate together and will match in the same way that full siblings match.

Please note that it’s possible for two of a woman’s eggs to be fertilized at different times during the same ovulation cycle, potentially by different men, resulting in twins who are actually half-siblings.

A difference in ethnicity is NOT a reliable indicator of fraternal or identical twins. Submitting your own DNA twice often results in slightly different ethnicity results.

Identical twins – Identical or monozygotic twins occur when one egg is fertilized by one sperm and then divides into multiple embryos that develop into different children. Those children are genetically identical since they were both developed from the same egg and sperm.

Two of the most famous identical twins are astronauts Mark and Scott Kelly.

Identical twins are the same sex and will look the same because they have the same DNA, except for epigenetic changes, but of course external factors such as haircuts, clothes and weight can make identical twins physically distinguishable from each other.

DNA testing companies will either identify identical twins as “self,” “identical twin” or “parent/child” due to the highest possible shared cM count plus fully matching FIR regions.

For identical twins, checking the FIR versus HIR is a positive identification as indicated above at GEDmatch with completely solid green FIR regions. Do not assume twins that look alike are identical twins.

Siblings

Whoever thought there would be so many kinds of siblings!

If you observe the need to educate about either sibling terminology or DNA identification methodologies, feel free to share this article. When identifying relationships, never assume anything, and verify everything through multiple avenues.

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Thank you so much.

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