Mitotree Q&A for Everyone

I recently presented Mitotree Webinar – What It Is, How We Did It, and What Mitotree Means to You at Legacy Family Tree Webinars. It’s still free to view through June 13th, and after that, it’s available in the webinar library with a subscription. The 31-page syllabus is also a subscription feature.

Thank you to all 1000+ of you who attended and everyone else who has since watched the webinar – or will now.

We had a limited amount of time for Q&A at the end, so Geoff, our host, was kind enough to send me the list of questions from the Chat, and I’m doing the Q&A here. But keep in mind, please, that I’m assuming when I answer that you’ve watched the webinar or are familiar with how the new Mitotree and tools work.

That said, I think this Q&A can help everyone who is interested in mitochondrial DNA. Your genealogy gift from your mother and her female lineage.

Just a quick reminder that the mitochondrial DNA test tracks your direct matrilineal line only, meaning your mother’s mother’s mother’s line on up your tree until you run out of mothers. Of course, our goal is always to break through that brick wall.

This is a wonderful opportunity, because, unlike autosomal DNA, mitochondrial DNA is not admixed with the DNA of the other parent, so it’s a straight line look back directly up your mother’s female line.

Aha Moment!

Geoff said at the end that he had an aha moment during the webinar. Both males and females have mitochondrial DNA inherited from their mother, so we think of testing our own – but forget to obtain the mitochondrial DNA of our father. Testing your father’s mitochondrial DNA means obtaining your paternal grandmother’s mitochondrial DNA, so test your father to learn about his mother’s maternal line.

And it’s Father’s Day shortly.

Q&A

I’ve combined and summarized similar questions to make this short and sweet. Well, as short and sweet as I can make anything!

  • Can I benefit from Discover even if I don’t have a full sequence test?

You can benefit from the free FamilyTreeDNA Discover tool with any haplogroup, even a partial haplogroup. Be sure to click the down arrow and select mtDNA before entering the haplogroup if you’re using the public version.

However, to gain the most advantage from your test results and Discover, and to receive your closest matches, you need the full sequence test, called the mtFull, which you can purchase here. If you took one of the lower-level “Plus” tests, years ago, click here to sign in and upgrade or check your account to see if you have the full sequence test.

  • What benefits do I receive if I click through to Discover from my account versus using the public version of Discover?

Click any image to enlarge

If you click through to Discover directly from your FamilyTreeDNA account, you will receive features and additional information that are not available in the free, public version of Discover.

You’ll receive additional Notable Connections and up to 30 Ancient Connections based on how many are available and relevant for you.

You’ll also be able to view the Match Time tree, showing your matches, their earliest known ancestors, and where they fit in your haplogroup and haplotype cluster. In this example, two EKAs hinted at a common lineage, which turned out to be accurate after I did some digging.

I think the Match Time Tree is indispensable – the best thing since sliced bread!

The Scientific Details report is also customized for you with your Haplotype Cluster and your private variants.

  • Will a child and their mother always have the same haplogroup?

Yes, but if one of them has a mutation that the other doesn’t, or a heteroplasmy, they may be in a different haplotype cluster.

Also, they both need to have taken the full sequence test. Otherwise, the one who did not take the full sequence test will only have a partial haplogroup until they upgrade.

We will talk more about edge cases in Q&A on down the list.

Great question. Sign in to your account.

In the Maternal Line Ancestry section, which is mitochondrial DNA, check to see if both the Plus and Full boxes are pink. If so, you have taken both and you’ll have a new Mitotree haplogroup and haplotype cluster.

If the “Full” box is grey, you can either click there or at the top where it says “Add Ons and Upgrades” to upgrade to the full sequence test.

  • Why is it called the Million Mito Project? What were you counting?

When we first launched the project, we hoped for a million full sequence samples to build the initial tree. After removing duplicates, such as parent/child, partial sequence samples such as HVR1/2, unreliable samples from PhyloTree, and including FamilyTreeDNA  testers and academic samples, we had between one-third and half a million samples when we launched. The Mitotree and Discover are growing with new testers and groups of samples from archaeological studies, academic samples, and other publicly available resources, following quality analysis, of course.

  • Is there a way to confirm that I submitted an mtDNA to the Mito Tree project? I think I submitted my mom’s when you first started, but my husband recently tested, and I don’t remember if we opted him in at that time.

The science team at FamilyTreeDNA  is using all of the full sequence tests in the construction of the Mitotree, so you don’t need to do anything special.

  • Do or can haplotype F numbers (haplotype clusters) ever become haplogroups?

The answer is maybe. (I know – I’m sorry!)

If you have private variants in addition to your haplotype cluster, then yes, those are haplogroup seeds.

This is my result and I have no additional private variants left to use.

If you don’t have any private variants, or mutations, left over, then no, you won’t receive a new haplogroup for this reason. However, if for some reason the haplogroup splits upstream, you might receive a new haplogroup in the future due to that split.

In addition to the webinar, I wrote about haplotype clusters in the article, Mitochondrial DNA: What is a Haplotype Cluster and How Do I Find and Use Mine?

  • How can mitochondrial DNA and the Mitotree be useful for breaking down genealogy in various parts of the world?

There are two aspects to mitochondrial DNA testing.

The first is to connect genealogically, if possible. To do that, you’ll be paying attention to your matches EKAs (earliest known ancestors), their trees, and their locations. You may well need to do some genealogy digging and build out some trees for others.

The second aspect is to learn more about that lineage before you can connect genealogically. Where did they come from? Do they share a haplogroup with any Ancient Connections, and what cultures do they share? Where did they come from most recently in the world, and where do the breadcrumbs back in time lead?

I wrote about this in the article, New Mitotree Haplogroups and How to Utilize Them for Genealogy.

Sometimes, DNA testing of any type is simply a waiting game until the right person tests and matches you. That’s one reason it bothers me so much to see people “not recommend” mitochondrial DNA testing. We all need more testers so we can have more matches.

  • When will Globetrekker for mtDNA be available?

I don’t know and neither does the team. The Mitotree is still being refined. For example, we are adding thousands of samples to the tree right now from multiple locations around the world. I probably wouldn’t expect Globetrekker until the tree is officially out of Beta, and no, I don’t know when that will happen either. It’s difficult to know when you’re going to be “finished” with something that has never been done before.

While it’s not Globetrekker, you do have the Matches Map to work with, and the Migration Map in Discover, which also shows the locations of your Ancient Connections.

  • During the webinar, Roberta mentioned that her ancestor is German, but she discovered her ancestors were Scandinavian. Can you expand about the “event” that explained this unexpected discovery.

In my case, the church records for the tiny village where my ancestor lived in Germany begin right after the 30 Years’ War, which was incredibly destructive. Looking at Swedish troop movements in Germany, the army of Gustavus Adolphus of Sweden marched through the region with more than 18,000 soldiers. Women accompanied the baggage trains, providing essential, supportive roles and services to the soldiers and military campaign. I’ll never know positively, of course, but given that the majority of my full sequence matches are in Scandinavia, mostly Sweden, and not in Germany, it’s a reasonable hypothesis.

People often receive surprises in their results, and the history of the region plays a big role in the stories of our ancestors.

You don’t know what you don’t know, until you test and follow the paths ahd hints revealed.

  • Why do I have fewer matches in the HVR2 region than the HVR1 region?

Think of the mitochondria as a clock face.

The older (now obsolete) HVR1 test tested about 1000 locations, from about 11-noon and the HVR2/3 region tested another 1000 locations, from about noon-1 PM. The full sequence test tests the full 16,569 locations of the entire mitochondria.

Each level has its own match threshold. So, if you have one mutation at either the HVR1 or HVR2/3 level, combined, you are not considered a match. For example, you can match 10 people at the HVR1 level, and have a mutation in the HVR2 level that 4 people don’t share, so you’ll only match 6 people at the HVR2 level.

If you have one mutation in the HVR1 region, you won’t match anyone in either the HVR1 or HVR1/HVR2 regions.

At the full sequence level, you can have three mutation differences (GD 3) and still be considered a match.

So, the short answer is that you probably have a mutation that some of your matches at the HVR2 level don’t have.

In addition to matches on your Matches page, you will (probably) have haplogroup matches that aren’t on your match list, so check Discover for those.

  • I have HVR1/HVR2 matches, but none at the full sequence level. Why?

It’s possible that none of your matches have tested at that level.

You have no mutations in the HVR1/2 region, or you would not be a match. If your HVR1/2 matches have tested at the full sequence level, then you have more than 3 mutations difference in the coding region.

  • Why do I match people at the full sequence level but not HVR1/2?

The match threshold at the HVR1/2 level is 1, so if you have one mismatch, you’re not listed as a match. However, at the full sequence level, the GD (genetic distance) is 3 mismatches. This tells me you have a mismatch in the HVR1 region, which also precludes HVR2 matching, but less than 4 mutations total. Click on the little “i” button above each match level on the matches page.

  • Why don’t all of my matches show on the Match Time Tree?

Only full sequence matches can show on the Match Time Tree, because they are the only testers who can receive a full haplogroup.

  • How does a heteroplasmy interfere with mtDNA research?

Heteroplasmies, where someone carries two different nucleotides at the same location in different mitochondrial in their body, are both extremely fascinating and equally as frustrating.

Heteroplasmies can interfere with your matching because you might have a T nucleotide in a specific location, which matches the reference model, so no mutation – like 16362T. Your mother might have a C in that location, so T16362C, which is a mutation from T to C. Your aunt or sister might have both a T and a C, which means she is shown with letter Y, so 16362Y, which means she has more than 20% of both. All three of you probably have some of each, but it’s not “counted” as a heteroplasmy unless it’s over 20%.

The challenge is how to match these people with these different values accurately, and how heteroplasmies should “count” for matching.

I wrote about this in the article What is a Heteroplasmy and Why Do I Care?

Bottom line is this – if you are “by yourself” and have no matches, or you don’t match known relatives exactly, suspect a heteroplasmy. If you ask yourself, “What the heck is going on?” – rule out a heteroplasmy. Check out my article and this heteroplasmy article in the FamilyTreeDNA help center.

  • Someone asked about the X chromosome and may have been confusing it with mitochondrial DNA. The X chromosome is not the same as mitochondrial DNA.

The confusion stems from the fact that both are associated with inheritance from the maternal line. Everyone inherits their mitochondrial DNA from their mother. Men inherit their X chromosome ONLY from their mother, because their father gives them a Y chromosome, which makes them a male. Females inherit an X chromosome from both parents. And yes, there are medical exceptions, but those are unusual.

I wrote about this in the article, X Matching and Mitochondrial DNA is Not the Same Thing.

  • How do you determine the location of the last mutation? A tester and their aunt are from one country, and another man in the same haplogroup is from another country, but he has tested only the HVR1/HVR2 level.

There are really two answers here.

First, you can’t really compare your full sequence new Mitotree haplogroup with a partial haplogroup based on only the HVR1/2 test. Chances are very good that if he upgraded to a full sequence test, he would receive a more complete haplogroup, and one that might be near the tester’s haplogroup, but perhaps not the same.

For example, my full sequence haplogroup is J1c2f. I have matches with people who only tested at the HVR1/HVR2 level, but they can only be predicted to haplogroup J, with no subgroup, because they are missing about 14,000 locations that are included in the full sequence test.

Using the Discover Compare feature, comparing haplogroup J to J1c2f clearly shows that the mutations that define haplogroup J1c2f happened long after the mutation(s) that define haplogroup J.

You can use other Discover tools such as the Match Time Tree (if you click through from your account), the Time Tree, the Ancestral Path and the Classic Tree to see when the various haplogroups were born.

  • My mother took the full sequence test in 2016, so should I look for an upgrade now? She is deceased so can’t retest.

First, I’m sorry for your loss, but so glad you have her DNA tests.

The good news is that you ordered the full sequence right away, so you don’t need to worry about an upgrade failing later. In this case, there is no upgrade because the full sequence tests all 16,569 locations.

Additionally, had you needed an upgrade, or wanted to do a Family Finder test, for example, FamilyTreeDNA stores the DNA vials for future testing, so you could potentially run additional tests.

And lastly, since we’re talking mitochondrial DNA, which you inherit from your mother with no admixture from your father, your mtDNA should match hers exactly, so you could test in proxy for her, had she not already tested.

  • Has anything changed in Native American haplogroups?

Absolutely. About 75% of testers received a new haplogroup and that includes people with Native American matrilineal ancestors.

For example, my Native ancestor was haplogroup A2f1a, formed about 50 CE and is now A2f1a4-12092, formed about 1600 CE, so has moved 2 branches down the tree and about 1500 years closer. My ancestor was born about 1683. Her descendant has 58 full sequence matches, 22 in the same haplogroup, and 16 people in their haplotype cluster.

I’m so excited about this, because it helps provide clarity about her ancestors and where they were before she entered my genealogy by marrying a French settler.

  • Are mtDNA mutations the same or similar to autosomal SNPs?

A SNP is a single nucleotide polymorphism, which means a single variation in a specific location. So yes, a mutation is a change in a nucleotide at a genetic location in Y-DNA, autosomal DNA, or mitochondrial DNA.

  • Can we filter or sort our matches by haplotype on our match page?

Not yet. Generally, your closest matches appear at or near the top of your match list. Of course, you can use the Discover Match Time Tree and you can download your matches in a CSV file. (Instructions are further down in Q&A.)

  • Is there a way to make it more obvious that the EKA should be in their matrilineal line? There are so many men as EKAs!

So frustrating. The verbiage has been changed and maybe needs to be revised again, but of course, that doesn’t help with the people who have already entered males. We know males aren’t the source of mitochondrial DNA.

When I see males listed as an EKA, I send the match a pleasant note. I’m not sure they make the connection between what they entered and what is being displayed to their matches. If they have included or linked to a tree, I tell them who, in their tree, is their mtDNA EKA.

I’ve written about how to correctly add an Earliest Known Ancestor. I’ll update that article and publish again so that you can forward those instructions to people with no EKA, or male EKAs.

  • I love learning about my ancient connections. I have a new match due to the updates, who is from a neighboring area to my great-great-great-grandmother.

I love, love, LOVE Ancient Connections. They tell me who my ancestors were before I have any prayer of identifying them individually. Then I can read up on the culture from which they sprang.

I’ve also had two situations where Ancient Connections have been exceptionally useful.

One is an exact haplogroup match to my ancestor, and the burial was in a necropolis along the Roman road about 3-4 km outside the medieval “city” where my ancestor lived.

In a second case, there were two villages in different parts of the same country, hundreds of miles apart, and one burial from about 200 years before my ancestor lived was found about 10 km from one of those villages. While this isn’t conclusive, it’s certainly evidence.

  • What does the dashed line on the Time Tree mean?

Dashed lines on the time tree can mean two things.

The red dashed line, red arrow above, is the haplogroup formation date range and correlates to the dates at the top of Time Tree, not show in this screen shot. You can also read about those dates and how they are calculated on the Scientific Details tab in Discover.

The brown dashed lines, green arrow above, connect an ancient sample to its haplogroup, but the sample date is earlier than the estimated haplogroup.

At first this doesn’t make sense, until you realize that ancient samples are sometimes carbon dated, sometimes dated by proximity to something else, and sometimes dated based on the dates of the cemetery or cultural dig location.

Archaeological samples can also be contaminated, or have poor or low coverage. In other words, at this point in time, the samples are listed, but would need to be individually reviewed before shifting the haplogroup formation date. Haplogroup formation dates are based on present day testers.

  • A cousin and I have been mtDNA tested. What might be gained by testing our other six female cousins/10 or so male cousins?

Probably not much, so here’s how I would approach this.

I would test one cousin who descends from another daughter of the EKA, if possible. This helps to sift out if a haplogroup-defining mutation has occurred.

If you or that cousin has private variants left over after their haplotype cluster is formed,  testing a second person from that line may well results in a new haplogroup formation for that branch.

I absolutely would ask every single one of those cousins to take an autosomal test, however, because you never know what tools the future will bring, and we want to leverage every single segment of DNA that our ancestors carried. Testing cousins in the only way to find those.

  • In the Mitotree, I am grouped in a haplogroup that, according to the Mitotree Match Time Tree, branched off only about 200 years ago and has four mtDNA testers in it, including me. In fact, my earliest known maternal line ancestor I found using pen-and-paper genealogy was indeed born around 230 years ago and is also the known maternal ancestor for one of these three testers – confirming the Mitotree grouping is correct. But the other two matches in this haplogroup are completely unknown to me. Unfortunately, they do not have a tree online, and they did not respond to several messages. Is there any way to find out more about them using the new Mitotree tools?

First of all, this is great news. Having said that, I share your frustration. However, you’re a genealogist. Think of yourself as a sleuth.

I’d start by emailing them, but in this case, you already have. Tell them what you know from your line and ask if their line is from the same area? End with a question for them to answer. Share tidbits from Discover – like Ancient Connections maybe. Something to peak their interest.

Next, put on your sleiuh hat. I’d google their name and email address, and check Facebook and other social media sites. I’d check to see if they match me, or any cousins who have tested, on an autosomal test. If they do match autosomally, use shared matching and the matrix tool. If they are an autosomal match, I’d also check other testing sites to see if they have a tree there.

  • One webinar attendee is haplogroup H1bb7a+151 and is frustrated because they only have eight matches and don’t understand how to leverage this.

Of course, without knowing more, I can’t speak to what they have and have not done, and I certainly understand their frustration. However, in mitochondrial and Y-DNA, you really don’t want thousands of matches. It’s not autosomal. You want close, good matches, and that’s what the Mitotree plus haplotype clusters provide.

Your personal goals also make a lot of difference.

For me, I wanted to verify what I think I know – and received a surprise. I also want to go further back if possible. Then, I want to know the culture my ancestors came from.

First, step through every single one of Discover’s 13 tools and READ EVERY PAGE – not skim. These are chapters in your free book about your ancestor.

Their haplogroup was formed about 1200, so all of those matches will be since that time. The Ancient Connections tell me it’s probably British, maybe Irish – but they will see more from their account than I can see on the public version of Discover.

The Time Tree shows me one haplotype cluster, which is where the tester’s closest matches will probably be, barring a mutation or heteroplasmy.

Looking at the matches, e-mail people, look for common locations in their trees, and see if any of them are also autosomal matches using the Advanced Matching tool.

Looking at the 10 success story examples I used, one man was able to connect 19 of his matches into three groups by doing their genealogy for them. This doesn’t work for everyone, but it will never work if we don’t make the attempt.

  • An attendee would like to search on the Earliest Known Ancestor’s (EKA’s) name field.

I would like that too. You can search on surnames, but that’s often not terribly useful for mitochondrial DNA. The Match Time Tree shows the EKA for all full sequence testers.

In the upper right hand corner of your Matches page, there’s an “Export CSV” file link. Click there to download in a spreadsheet format. The EKA is a column in that file, along with both the new Mitotree haplogroup and haplotype F number, and it’s very easy to do a sort or text search from there.

  • Several questions about why people have so many more autosomal matches than either Y-DNA or mitochondrial.

There are several considerations.

First, autosomal testing became very popular, often based on ethnicity. There are many times more autosomal testers than there are either Y or mitochondrial.

Second, if you look back just six generations, you have 64 lineages. Y-DNA and mtDNA tests one line each and you don’t have to figure out which line. It also reaches back much further in time because it’s not admixed, so nothing washes out or rolls off in each generation like with autosomal.

Third, the Y-DNA and mitochondrial DNA tests are very specific and granular.

More is not necessarily better. You’re looking for refinement – and mitochondrial is just one line. No confusion. Think how happy you’d be if your autosomal matches weren’t all jumbled together and could be placed into 64 neat little baskets. Think how much time we spend sorting them out by shared matches and other criteria. Both Y-DNA and mitochondrial is already sorted out.

I’ve broken through several brick walls with unrecombined Y-DNA and mitochondrial DNA that could never be touched with autosomal – especially older lines where autosomal DNA is either gone or negligible.

  • You mentioned a Facebook group where I can ask questions about mitochondrial DNA?

The mitochondrial DNA Facebook group is the FamilyTreeDNA mtDNA Group, here.

  • To the webinar attendee who came to see me more than 20 years ago at Farmington Hills, Michigan, at one of my first, if not the first, genetic genealogy presentation – thank you!

Thank you for attending then when I really had no idea if ANYONE would come to hear about this new DNA “thing” for genealogy. I remember how nervous I was. And thank you for sticking around, continuing to research, and saying hello now!

Closing Comment

Mitochondrial DNA testing is different than autosomal, of course. It’s often the key to those females’ lines with seemingly insurmountable brick walls.

I attempt to collect the mitochondrial DNA of every ancestor. I trace “up the tree” to find people to test who descend from those ancestors through all women to the current generation, which can be males.

To find testers, I shop:

  • Autosomal matches at FamilyTreeDNA
  • Projects at FamilyTreeDNA
  • WikiTree
  • FamilySearch
  • Ancestry DNA matches
  • Ancestry Thrulines
  • Ancestry trees
  • MyHeritage DNA matches, where ther are a lot more European testers
  • MyHeritage Theories of Family Relativity
  • MyHeritage Cousin Finder
  • Relatives at RootsTech during the month before and after RootsTech when it’s available
  • Facebook Genealogy and family groups that appear relevant

When I find an appropriately descended person, I ask if they have already taken either the Y-DNA or mitochondrial DNA test, whichever one I’m searching for at that moment. If yes, hurray and I ask if they will share at least their haplogroup. If they haven’t tested, I tell them I’m offering a testing scholarship.

I will gladly explain the results if they will share them with me. Collaboration is key and a rising tide lifts all ships.

My mantra in all of this is, “You don’t know what you don’t know, and if you don’t test, you’ll never know.” I’ve missed testing opportunities that I desperately wish I hadn’t, so test your DNA and find testers to represent your ancestors.

I hope you enjoyed the webinar. It’s not too late to watch.

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The Mystery of the Blue Fugates and Smiths: A Study in Blue Genes and Pedigree Collapse

The story of the Blue Fugates, an Appalachian family, is quite interesting, from a genetic perspective, a genealogical perspective, and a genetic genealogy perspective.

Who Are the Blue Fugates?

Martin Fugate, supposedly an orphan from France, and his bride, Elizabeth Smith, who had married by 1840, have long been attributed as the progenitors of the Blue Fugate Family of Troublesome Creek, in and around Perry County, Kentucky.

Their descendants were known as “The Blue Fugates” and also “The Blue People of Kentucky” because some of their children and descendants carried a recessive autosomal genetic trait, Methemoglobinemia.

Methemoglobinemia causes the skin to appear blue due to an oxygen deficiency in the red blood cells. Some people only exhibit this characteristic, or even just blue tinges in their fingernails and lips, when they are cold or agitated, such as when infants cry. Yet others are very, very blue.

Inheritance

In order for someone to exhibit the autosomal recessive trait of blueness due to Methemoglobinemia, they must inherit a copy of the gene from BOTH PARENTS. That’s why this trait is so rare.

  • If the parents have only one copy each, they are carriers and will not have the condition themselves.
  • If one parent carries either one or two copies, and the other parent does NOT carry a copy, their offspring CANNOT carry two copies of the mutation and will not be blue.
  • If both parents carry a copy, and both parents pass their copy on to their offspring, the offspring will probably exhibit some level of blueness – from just a tinge when they are cold, ill or or upset, to very, very blue.

I’m not a physician, so I’m not delving into the medical specifics of Methemoglobinemia, but suffice it to say that levels of 10-20% of methemoglobin in the blood produce blue skin, higher levels can produce more severe medical conditions, and levels beneath that may not be visually detectible.

What’s important for the genealogy aspect of this story is that both parents must carry a copy AND pass their copy on for the condition to express in their offspring.

We’ve learned a lot since the 1800s when this was first observed in various members of the Fugate family in Perry County, KY, and since the 1960s when this phenomenon was first studied in the Fugate family and their descendants. To be clear, there are also references to the blue Combs and blue Ritchies in and around Perry County – but the common factor is that they have ancestors that descend from the Fugate family AND the Smith family ancestors, both.

During my research, I’ve proven some of what was initially accepted as fact was incorrect – and I’d like to correct the record. Bonus points too, because it’s just such a great genealogy story!

My Interest

I’ve been inordinately interested in the Fugate family for a long time – but not because of their famous blueness.

The Fugate family has been found for more than 225 years alongside my Cook, Claxton, Campbell, and Dobkins families. First, in Russell County, VA, where Josiah Fugate was granted land along Sword’s Creek in 1801 that adjoined Harry Smith, Richard Smith, and others, including my brick-wall ancestor, Joel Cook. Keep in mind that we have never discovered the birth surname of Joel’s wife or Joel’s parents.

Joel’s daughter, Sarah, married James Claxton about 1799 or 1800 in Russell County, and in February of 1802, James Claxton and Zachariah Fugate, among others, were ordered to view and lay out a new road. They were clearly neighbors, living on the same road, and knew each other well. We don’t know who James’ parents were either.

The Fugates first lived adjacent to the Cook, Riley, Stephens, and Claxton families on Mockason Creek in Russell County, then later migrated with the same group of families to Claiborne County where they lived along the Powell River near the Lee County, VA line, and are very closely associated with the Dobkins and Campbell lines.

Sometime between 1802 and 1805, several Russell County families moved 110 miles down the mountain range and settled together on the Powell River in Claiborne County, TN.  About the same time, others from the same cluster moved to what would eventually become Perry County, KY.

In 1805, the Fugates were ordered as road hands on the north side of Wallen’s Ridge in Claiborne County, the part that would become Hancock County in the 1840s, along with James Claxton and several Smiths.

In 1808, James Claxton witnessed a deed to Henley Fugate and John Riley.

The unsubstantiated family rumor, repeated as fact but with no source, has always been that William Fugate married the sister of my John Campbell. If that were true, tracking the Fugates would help me track my Campbells – yet another brick wall. Hence, my early interest in the Fugate family. Until now, I’ve never solved any part of that puzzle.

In 1827, in Claiborne County, Henry Cook, road overseer, is assigned John Riley, Henly Fugate, William Fugate, Fairwick Claxton (son of James who had died in 1815), and others. These families continued to be allied, living close to each other.

In 1842, William Fugate (1799-1855), born to William Fugate and Sarah Jane Stephens in Russell County, is involved in the estate of John Campbell, born about 1772, who had died in 1838. John Campbell was the husband of Jane “Jenny” Dobkins, daughter of Jacob Dobkins (1751-1835).

William Fugate of Claiborne County signed a deposition in 1851 saying he came to Claiborne County, TN, in 1826. Claiborne County is rugged terrain, located on the south side of the Cumberland Gap, where Virginia, Tennessee, and Kentucky intersect.

In 1853, both William Fugate and Jehiel Fugate are neck-deep in lawsuits surrounding the estate of Jacob Dobkins, who died in 1835, lived on Powell River, and whose daughters married John Campbell and his brother George Campbell

I recently discovered that this William Fugate was born about 1799 in Russell County, VA, and according to his son’s death certificate, William’s wife was Nancy Riley, which makes a lot of sense, given the proximity of these families. I must admit, I’m glad to solve this, but I’m also disappointed that he wasn’t married to John Campbell’s sister.

So, why does any of this matter in the Blue Fugate story?

In part, because I knew decades ago that Martin Fugate, of the Kentucky Blue Fugates, was not an orphan from France who had somehow made his way to the eastern shores of Maryland, then to Perry County, KY by 1820 when he supposedly received a land grant. That land grant date doesn’t square with Martin’s birth year of 1820 either, nor his marriage about 1840, both of which are substantiated by the census.

You can see from the information gleaned from Russell County that the Fugate family was there well before 1800. In fact, a Martin Fugate is shown on the 1789 tax list and other Fugates were there earlier, as early as 1771, according to extracted Russell County records in the book “The Fugate Family of Russell County, Virginia” by David Faris. The Fugate descendants continued to press on westward from there. Fugate, unlike Smith, Cook, and even Campbell, is not a common surname.

“Orphan” stories are often early ways that people said “I don’t know”, without saying, “I don’t know where he came from”, so they speculated and said “maybe he was an orphan.” Then that speculation was eventually passed on as fact.

That might have been happening in Perry County in the 1960s, but in Claiborne County in the 1980s, family members were telling me, “Martin waren’t no orphan,” and would roll their eyes and sigh with great exasperation. You could tell this was far from the first time they had had to combat that story. To be clear, the Fugate family lived down along Little Sycamore Creek with my Estes, Campbell and other ancestral families. In the 1980s, I was finding the oldest people possible and talking to them.

Some records in Russell County, where the Fugates of Perry County, KY, and the Fugates of Claiborne County, TN, originated, did and do exist, so could have been researched in the 1960s, but you would have had to know where to look. No one back then knew that the Perry County Fugates originated in Russell County, so they wouldn’t have known to look there. Research wasn’t easy. If they had known to look in Russell County, they would have had to travel there in person to review records. Early records exist in Perry County, too, but in the 1960s, not even the census was available, and people simply didn’t remember back to the early to mid-1800s.

Truthfully, no one would ever have doubted those early stories that had been handed down. They were revered, in all families, and treated as gospel. Those stories were the only connection they had to their ancestors – and the generations inbetween who passed them on. Nope, no one was going to question what Grandpa or Uncle Joe said.

So, in the 1960s, when the Blue Fugates in Perry and adjacent Breathitt County, KY were first studied by Dr. Cawein and his nurse, Ruth Pendergrass, they gathered oral family history and constructed a family pedigree from that information. They documented who was blue from first-hand eye-witness accounts – which would only have stretched back into the late 1800s, best case.

It probably never occurred to anyone to validate or verify earlier information that was provided. Plus, it would have been considered rude. After all, they weren’t genealogists, and they were trying to solve a medical mystery. The information they collected did not conflict with what was known about the disease and how it was transmitted, so they had no reason to doubt its historical accuracy.

The Mystery of the Blue Fugates?

The Blue Fugates were a family renowned for their blue skin – at least some of them had blue skin. That’s part of what makes this story so interesting.

Originally, it was believed that only one progenitor couple was involved, Martin Fugate and his wife, Elizabeth Smith, but now we know there were two. Maybe I should say “at least two.”

Martin Fugate and his bride, Elizabeth Smith, whose first known child was born in 1841, according to the 1850 census, are progenitors of the Blue Fugate Family of Troublesome Creek, but they aren’t the only progenitors.

Martin was not shown in the Perry County, KY 1840 census, but two Zachariah Fugates are present, 8 Fugate families are found in neighboring Breathitt County, more than a dozen in Russell County and surrounding counties in Virginia, and four, including two William Fugates, in Claiborne County, TN. The younger of the two lived next door to John Dobkins, son of deceased Jacob Dobkins.

Martin Fugate (c1820-1899) of Perry County and his second cousin, Zachariah Fugate (1816-1864), who each married a Smith sister, are both progenitors of the Blue Fugates through their common ancestor, their great-grandfather, Martin Fugate, who was born in 1725 and died in 1803 in Russell County, VA.

Obviously, if Martin (c1820-1899) had a Fugate second cousin who also lived in Perry County, Martin wasn’t an orphan. That knowledge is due to more recently available information, like census and other data – and that’s part of what I want to correct.

In 1948, Luke Combs, from Perry County, KY, took his sick wife to the hospital, but Luke’s blueness caused the medical staff to focus on him instead, thinking he was experiencing a medical emergency. He wasn’t. His skin was just blue. In 1974, Dr Charles H. Behlen II said, ‘Luke was just as blue as Lake Louise on a cool summer day.’ The Blue Fugates were “discovered” by the rest of the world, thanks to Luke, but they were nothing new to local people, many of whom did not welcome the notoriety.

In the 1960s, hematologist Madison Cawein III, with the assistance of Ruth Pendergrass, studied 189 members of the extended Fugate family, treated their symptoms, and published his findings. He included a pedigree chart, but not everyone was keen on cooperating with Dr. Cawein’s research project.

The Fugate family history collected for the study was based on two things:

  • Personal knowledge of who respondents knew was blue
  • Remembered oral history beyond the reach of personal knowledge.

That remembered oral history reported that Martin Fugate and Elizabeth Smith’s youngest son, Zachariah Fugate (born in 1871), married his mother’s (older) sister, Mary Smith, (born about 1820), and had a family. I’ve added the dates and information in parentheses, or they would have immediately known that marriage was impossible. Or, more directly, even if they married when Zachariah was 14, Mary would have been 70 years old, and they were certainly not going to produce offspring. This is the second piece of information I want to correct. That marriage never happened, although people were accurate that:

  • Martin Fugate and his wife, Elizabeth Smith, did have a son named Zachariah Fugate
  • One Zachariah Fugate did marry Mary Smith, sister of Elizabeth Smith

It’s just that they were two different Zachariah Fugates, born 75 years apart. Same name confusion strikes again.

I constructed this census table of Martin Fugate with Elizabeth Smith, and Zachariah Fugate with Mary Smith. They lived next door to each other in Perry County – and it seemed that every family reused the same “honoring” names for their children – and had been doing such for generations.

In the 1960s, when the information was being compiled for Dr. Cawein, the census and other documents that genealogists rely on today were not readily available.

Furthermore, genetically, for the mystery Dr. Cawein was attempting to solve, it didn’t really matter, because it was still a Smith female marrying a Fugate male. I know that it made no difference today, but he wouldn’t have known that then. To track down the source of the blueness, he needed to identify who was blue and as much about their ancestors as possible.

The Zachariah Fugate (1816-1864) who married Elizabeth Smith’s sister, Mary Smith, was Martin Fugate’s second cousin by the same name, Zachariah. Both Martin (c1820-1899) and his second cousin, Zachariah (c1816-1864), married to Smith sisters, had blue children, which helps cement the fact that the responsible genes were passed down through BOTH the Fugate and Smith lines, and weren’t just random mutations or caused by environmental or other factors.

Proof

In case you’re wondering exactly how I confirmed that Martin and Zachariah did indeed marry Elizabeth and Mary Smith – their children’s birth and death records confirmed it. These records correlate with the census.

Unlike most states, Kentucky has some pre-1900 birth and death records.

Wilson Fugate’s birth in February, 1855 was recorded, naming both of his parents, Martin Fugate and Elizabeth Smith.

Martin Fugate and Elizabeth Smith’s son, Henley or Hendley, died in 1920, and his death certificate gave the names of both parents. Betty is a nickname for Elizabeth.

On the same page with Wilson Fugate’s birth, we find a birth for Zachariah Fugate and Mary Smith, too.

Hannah Fugate was born in December 1855.

Zachariah Fugate and Mary Smith’s son, Zachariah died in 1921, and his death certificate gives his parents as Zach Fugate and Polly Smith, a nickname for Mary.

There are more death records for children of both sets of parents.

Both couples, Martin Fugate and Elizabeth Smith, and Zachariah Fugate and Mary Smith, are progenitors of the Blue Fugate family.

Of Martin’s 10 known children, 4 were noticeably “blue” and lived long, healthy lives. At least two of Zachariah’s children were blue as well.

Some people reported that Martin, himself, had deep blue skin. If so, then both of his parents would have carried that genetic mutation and passed it to him.

Unfortunately, color photography didn’t exist when Martin (c1820-1899), lived, so we don’t know for sure. For Martin’s children to exhibit blue skin, they would have had to inherit a copy of the gene from both parents, so we know that Martin’s wife, Elizabeth, also inherited the mutation from one of her parents. Ditto for Zachariah Fugate and Mary Smith. The chances of two families who both carry such a rare mutation meeting AND having two of their family members marry are infinitesimally small.

Dr. Cawein’s Paper

In 1964, Dr. Cawein published his findings, but only with a pedigree chart with no names. What was included was an explanation about how remote and deep the hills and hollows were, and that out-migration was almost impossible, explaining the propensity to marry cousins.

Legend:

  • Measured – Found to have elevated methemoglobin
  • Measured – Found to have decreased methemoglobin
  • Not measured – Reported to be “blue”
  • Measured – Found to be normal

Cawein further stated that data was collected by interviewing family members who personally knew the individual in question and could say if they were actually blue.

Cawein erroneously reported that “Martin Fugate was an orphan born about 1800, landed in Maryland, obtained a land grant in Perry County, KY in 1820, and married a local gal. From 1820 to about 1930, the population consisted of small, isolated groups living in creek valleys and intermarriage was quite common.” Bless his heart.

Later, geneticist Ricky Lewis wrote about the Blue Fugates, sharing, among other things, the provenance of that “blue” family photo that circulates on the internet, revealing that it is a composite that was assembled and colorized back in 1982. She also erroneously stated that, “after extensive inbreeding in the isolated community—their son married his aunt, for example—a large pedigree of “blue people” of both sexes arose.” Bless her heart too.

Dr. Lewis is incorrect that their son married his aunt – but she’s right that intermarriage between the families is responsible for the blue descendants. In colonial America, and elsewhere, cousin marriages were fairly common – everyplace. You married who you saw and knew. You saw your family and neighbors, who were generally your extended family. No left-handed apology needed.

Pedigree collapse, sharing the same ancestors in multiple places in your tree, is quite common in genealogy, as is endogamy among isolated populations.

Today, things have changed somewhat. People move into and out of an area. The younger generation moves away a lot more and has for the past 100+ years. Most people know their first cousins, but you could easily meet a second or third cousin and never know you were related.

While early stories reported that Martin Fugate (c1820-1899) was an orphan from France, mysteriously appearing in Kentucky around 1820, later genealogical evidence as well as genetic research proves that Martin Fugate was actually born about 1820, in Russell County, VA and his ancestors, over several generations, had followed the typical migration path across Virginia into Kentucky.

We’ve also proven that Martin’s son, Zachariah (born 1871) was not the Zachariah who married Elizabeth Smith’s sister, Mary, who was 50 years old when Zachariah was born.

What else do we know about these families?

The Back Story

Compared to the Smith story, the Fugate story was “easy.”

Don’t laugh, but I spent several days compiling information and charting this in a way I could see and understand in one view.

I hesitate to share this, but I’m going to because it’s how I think. I also put together a very basic Fugate tree at Ancestry, here. Many children and siblings are missing. I was just trying to get this straight in my mind.

Click to enlarge any image

This spreadsheet is color-coded:

  • The text of each lineage has a specific color. For example, Fugates are blue.
  • Some people (or couples) are found in multiple descendants’ lines and are duplicated in the tree. Duplicated people also have a cell background color. For example, Mahala Richey (Ritchey, Ritchie) is highlighted yellow. James and Alexander Richey have green text and apricot background because they are duplicated.
  • The generation of parents who had blue children is marked with black boxes and the label “Blue Kids.”
  • Only the blue kids for this discussion are listed below those couples.
  • The bluest person was Luna Fugate (1886-1964).
  • While Luna’s husband, John Stacey, also descended from the Smith/Combs line, only one of their children expressed the blue trait. That child’s lips turned blue when they cried. John and Luna were actually related in three ways. Yes, my head hurts.
  • The last known “blue” person was Luna Fugate’s great-grandchild, whose name I’ve obfuscated.

Ok, let’s start with the blue Fugates on our spreadsheet. You’ll probably want to follow along on the chart.

Martin Fugate (1725-1803) and wife Sarah, had several children, but only two, the ones whose grandchildren married Smith sisters are known to have had blue children.

On our chart, you can see that Martin (1725-1803) is blue, and so is Son 1, William Fugate and Sarah Stephens, along with Son 2, Benjamin Fugate and Hannah Devers. Both William and Benjamin are mentioned in Martin’s estate in 1803 in Russell County, VA.

Two generations later, Martin Fugate (c1820-1899) and Elizabeth Smith had four blue children, and Zachariah Fugate (c1816-1864) and Mary Smith had at least two blue children. Furthermore, Zachariah Fugate’s sister, Hannah (1811-1877), married James Monroe Richie.

The Richey’s are green, and you can see them on both the left and right of the chart. Hannah’s husband descended from the same Richey line that Elizabeth Smith did. It was no surprise when their child, Mahala Ritchie (1854-1922), married Levi Fugate, to whom she was related three ways, they became the parents of a blue child. Their daughter, Luna Fugate, was known as “the Bluest of the Blue Fugates.”

Mahala Ritchie (1854-1922) could have inherited her blue gene (or genes) from either her mother Hannah Fugate, or her father, James Monroe Ritchie, or both. We don’t know if Hannah was blue or not.

We do know that Mahala married Levi Fugate, her third cousin through the Fugate line, and her third and fourth cousin also through the Richie and Grigsby lines, respectively. This is the perfect example of pedigree collapse.

You can see the purple Grigsby lines in the center and to the right of the pedigree chart too, with Benjamin Grigsby, highlighted in blue, being common to both lineages.

Zachariah Fugate (1816-1864) and Mary Smith had at least two blue sons, but I am not tracking them further. Suffice it to say that Blue John married Letha Smith, his first cousin, the granddaughter of Richard Smith and Nancy Elitia Combs. Lorenzo, “Blue Anze”, married a Fugate cousin, so it’s no surprise that Zachariah and Mary were also progenitor couples of the Blue Fugates.

Martin’s son, Levi Fugate, married Mahala Ritchie, mentioned above, and had Luna Fugate who would have been personally known to Dr. Cawein. Luna, pictured above, at left, was known as the bluest of the Blue Fugates.

Luna married John Stacey who some thought wasn’t related to Luna, so it was confusing why they had one child that was slightly blue. However, John turns out to be Luna’s second cousin, third cousin once removed and first cousin once removed through three different lines. His great-grandparents were Richard Smith and Nancy Combes. Since one of their children had a slight blue tinge, John, while not visibly blue himself, clearly carried the blue gene.

Where Did the Blue Gene Come From?

The parents of Elizabeth Smith and Mary Smith were Richard Smith and Nancy (Eletia) Combs. His Smith ancestors include both the Richeys and Caldwells.

James Richey (1724-1888) married Margaret Caldwell (1729-1802) and his father, Alexander Richey (1690-1749) married Jeanne Caldwell (1689-1785). While the Caldwell females weren’t closely related, Jeanne was the daughter of Joseph Alexander Caldwell (1657-1730) and Jane McGhie, and Margaret Caldwell (1729-1802) was the great-granddaughter of that couple. The Caldwells are shown in magenta, with both Richey/Caldwell couples shown as duplicates. The Richey are highlighted in apricot, and the Caldwell’s with a light grey background. It was difficult to show how these lines connect, so that’s at the very top of the pedigree chart.

When just viewing the Smith-Combs line, it’s easier to view in the Ancestry pedigree.

The Smith, Richey, Combs, Grigsby, and Caldwell lines are all repeated in different locations in the trees, such as with Hannah Fugate’s husband. These repeated ancestors make it almost impossible for us to determine where in the Smith ancestral tree that blue gene originated.

We don’t know which of these ancestral lines actually contributed the blue gene.

Can We Figure Out Where the Blue Gene Came From?

How could we potentially unravel this mystery?

We know for sure that the blue gene in the Fugate side actually descends from Martin Fugate who was born in 1725, or his wife, Sarah, whose surname is unknown, because their two great-grandchildren, Martin (c1820-1899) and Zachariah (1816-1864) who both married Smith sisters had blue children. For those two intervening generations between Martin Fugate (1725-1803) and those two great-grandsons, that blue gene was quietly being passed along, just waiting for a blue Fugate gene carrier to meet another blue gene carrier. They found them in the Smith sisters.

None of Martin (1725-1803) and Sarah’s other children were known to have had any blue children or descendants. So either they didn’t carry the blue gene, or they didn’t marry someone else who did – that we know of.

We can’t tell on the Smith side if the blue gene descends from the Smith, Richey, Grigsby or Caldwell ancestors, or maybe even an unknown ancestor.

How can we narrow this down?

If a Fugate in another geographic location married someone from one of these lineages, say Grigsby, for example, and they had blue offspring, and neither of them shared any of the other lineages, then we could narrow the blue gene in the Smith line to the Grigsby ancestor.

Unfortunately, in Perry and surrounding counties in Kentucky, that would be almost impossible due to intermarriage and pedigree collapse. Even if you “think you know” that there’s no connection through a third line, given the deep history and close proximity of the families, the possibility of unknown ancestry or an unexpected parent is always a possibility.

Discover

While the blue gene is not connected to either Y-DNA or mitochondrial DNA, we do have the Fugate’s Y-DNA haplogroup and the Smith sisters’ mitochondrial DNA.

Y-DNA

The Big Y-700 haplogroup for the Martin Fugate (c1820-1899) line is R-FTA50432, which you can see, here..

You can see the Blue Fugate Family by clicking on Notable Connections.

If you’re a male Fugate descendant who descends from anyone other than Martin Fugate (c1820-c1899), and you take a Big Y test, you may well discover a new haplogroup upstream of Martin (c1820-1899) that represents your common Fugate ancestor.

If you descend from Martin, you may find youself in either of the two haplogroups shown for Martin’s descendants, or you could split the line to form a new haplogroup.

We don’t have the mitochondrial DNA of Martin Fugate (c1820-1899), which would be the mitochondrial DNA of his mother, Nancy Noble. We also don’t have the the mtDNA of Mary (Polly) Wells, the mother of Zachariah Fugate (c1816-1864). If you descend from either of these women in a direct matrilineal line, through all women, please take a mitochondrial DNA test and reach out. FamilyTreeDNA will add it as a Notable Connection.

We do, however, have the mitochondrial DNA of Elizabeth and Mary Smith

Mitochondrial DNA of Elizabeth and Mary Smith

The mitochondrial DNA of both Elizabeth and Mary Smith follows their mother’s line – Nancy Combs through Nancy (Eletia?) Grigsby. Nancy’s mother is unknown, other than the possible first name of Margaret.

Nancy Grigsby’s descendant is haplogroup K1a61a1, which you can see here.

The Blue Fugates show under Notable Connections.

The Smith sisters’ haplogroup, K1a61a1, tells us immediately that their ancestor is European, eliminating other possibilities.

The time tree on Discover is quite interesting

Haplogroup K1a61a1 was formed about the year 1400. Descendants of this haplogroup are found in the UK, Scotland, England, several unknown locations, and one person who selected Native American, which is clearly in error. Haplogroup K is not Native American.

By focusing on the haplotype clusters, identified by the F numbers in the elongated ovals, our tester may be able to identify the mother of Nancy Grigsby, or upstream lineages that they can work back downstream to find someone who married Thomas Grigsby.

This story is far from over. In fact, a new chapter may just be beginning.

If you’re a Fugate, or a Fugate descendant, there’s still lots to learn, even if autosomal DNA is “challenging,” to say the least, thanks to pedigree collapse. Testing known females lineages can help us sort which lines are which, and reveal their hidden stories.

Other resources if you want to read more about the Fugates: The Blue People of Troublesome Creek, Fugates of Kentucky: Skin Bluer than Lake Louise, Those Old Kentucky Blues: An Interrupted Case Study, and Finding the Famous Paintings of the Blue People of Kentucky.

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Leave No Stone Unturned, No Ancestor Behind: 10 Easy Steps to Capture DNA Clues

There’s a lot, a whole lot that DNA testing can tell you. Not just your own tests, but the genetic information carried by your relatives that you do not.

Recently, I’ve been reviewing my brick walls, which led me to realize there are several ancestors who are missing their mitochondrial DNA and/or Y-DNA  results. I need these to learn more about my ancestors that can’t be revealed any other way – and to break down those pesky brick walls.

I’ve solved two mysteries recently, one thanks to a Big Y-700 test, and a second very unexpectedly thanks to mitochondrial DNA – both thanks to cousins who tested. These revelations were very encouraging, especially since there’s no way other than DNA for me to break through these brick walls. The mitochondrial test had been sitting there, waiting for what seemed like forever until just the right other person tested.

I am in the process of unlocking several brick-walled ancestors by providing testing scholarships to people who are appropriately descended from known ancestors in those lines.

Don’t leave information on the table. If I were to tell you there even MIGHT be a book available about your family, you’d overturn Heaven and Earth to find it – but you don’t need to do that. All you need to do is order DNA tests for cousins.

All cousins can provide useful autosomal DNA results, but you do need to find appropriate cousins for Y-DNA and mitochondrial DNA testing.

I’m sharing the steps for how I accomplish this! You’ll be amazed at what’s out there – and someone may already have tested!

Take Advantage of the Holidays

I’m sharing NOW because it’s the holidays and you’re likely to gather with people you don’t see any other time – and because the best sale of the year for both Y-DNA and mitochondrial DNA lasts from now through the end of the year.

These two factors combined mean strike while the iron is hot.

Prices for new tests and bundles are at an all-time low.

If you or your relatives have already taken a lower-level test, now is the time to upgrade to either the Big Y-700 or the mtFull Sequence test.

Step 1 – Test Yourself and Your Known Family

If you’re a male, order both the Big Y-700 test and mitochondrial DNA tests.

Be sure to click on “See More” for more useful tools.

When you receive your results, be sure to click on all of the tabs in your results, and do the same by clicking through to Discover from your account. Discover has 13 more goodies for you to help with your genealogy.

Both your personal page and Discover are essentially chapters of your own personal book about your DNA results. 25 very interesting chapters, to be precise, that are uniquely you.

I’ve written about understanding Y-DNA results here, and mitochondrial results here. My book, Complete Guide to FamilyTreeDNA, covers both along with Discover.

Discover provides robust information for Y-DNA haplogroups. If you’ve taken a Big Y-700 test, you’ll want to click through from your page to receive additional, personalized and more robust information than is available through the free public Discover tool. That said, the public version of Discover is an amazing tool for everyone.

After the new Mitotree is released for mitochondrial DNA, mitochondrial haplogroups will be available in Discover too.

I can’t even begin to stress how important these tools are – in particular the Time Tree, the Group Time Tree for members of group projects, and the Match Time Tree for your own matches.

Who Can Test For What?

Once you’ve tested yourself, you will want to take a look in your pedigree chart at branches further up your tree to see who can be tested to represent specific ancestors.

Let’s begin with my father’s side.

A mother contributes her mitochondrial DNA to all of her children, so your father carries the mitochondrial DNA of his mother.

If you’re a female, and your father is available to test, you’ll want to test BOTH his mitochondrial DNA and Y-DNA, because there’s no way for you to obtain that information from your own test. Females don’t have a Y chromosome, and men don’t pass on their mitochondrial DNA.

If you’re a male, you can test your own mitochondrial DNA and Y-DNA, but you’ll need to test your father’s mitochondrial DNA to obtain his mother’s. You might still want to test your father’s Y-DNA, however, because you may discover a personal family haplogroup. How cool is that??!! Your own tiny branch on the tree of mankind!

Your father’s mitochondrial DNA provides you with mitochondrial matches and haplogroup information for your paternal grandmother – in this case, Ollie Bolton.

If your father and his siblings can’t test, then all of the children of your paternal aunts carry your paternal grandmother’s mitochondrial DNA.

If they have no children or they can’t test, then the children of Ollie Bolton’s mother, Margaret Claxton/Clarkson all carry her mitochondrial DNA, and the children of Ollie’s sisters continue the line of descent through all daughters to the current generation.

The male children of Joseph “Dode” Bolton and Margaret Claxton carry his Y-DNA. Fortunately, that’s not one of our missing haplogroups.

Yes, you may have to climb up your tree and climb down various branches to find a testing candidate.

One of the reasons I’m using this example is because, while I have a high-level haplogroup for my grandmother, Ollie Bolton, we need a full sequence tester – and I’m offering a mitochondrial DNA testing scholarship for anyone descending from Margaret Claxton (or her direct female ancestors) through all females to the current generation, which can be male.

Ok, now let’s switch to the maternal side of your tree.

On the other side of your tree, your maternal grandfather or your mother’s brothers will provide the Y-DNA of your mother’s father’s line. Your mother’s uncles or their sons will provide your grandfather’s Y-DNA line, too. In this case, that’s John Whitney Ferverda, who carries the Y-DNA of his father, Hiram Bauke Ferverda/Ferwerda.

Your maternal grandfather or his siblings will provide the mitochondrial DNA of their mother, Evaline Louise Miller.

If they are deceased or can’t test, for mitochondrial DNA, look to the children of Evaline Miller’s daughters or their descendants through all females to the current generation, which can be male.

And yes, in case you’re wondering, I do need Evaline Miller’s mitochondrial line too and am offering a scholarship.

You might have noticed that I’ve been inching my way up my tree. All of my immediate relatives have passed over already, so I’m now looking for testers that I don’t know but who I’m related to.

If you’re seeing family members anytime soon, figure out if their Y-DNA, mitochondrial DNA, or autosomal DNA would be useful for your common genealogy. Take advantage of the opportunity.

Next, you’ll want to figure out which ancestors need haplogroups and locate appropriate cousins.

Step 2 – Identify Ancestors Who Need Haplogroups

Peruse your tree to determine which of your ancestors you need haplogroup information for. To make it easy, on my computer, but never in a public tree anyplace, I store the haplogroup of my ancestor as a “middle name” so I can easily see which ones I have and which ones I need. Sometimes, I have a high-level haplogroup and either need a new tester or someone to upgrade.

Sometimes, I have one tester from a line but need a second for confirmation.

In this example, I’m not missing confirmation on any Y-DNA haplogroups (although I am further upstream on different lines,) but I do need four different mitochondrial DNA lineages.

For easy reference, make a list of all of the lines you can’t confirm with two testers from different children of the same ancestor.

You just might get lucky and discover that someone has already tested!

Step 3 – Check FamilyTreeDNA Projects

Check FamilyTreeDNA Projects to see if someone has already tested to represent those ancestors on your list.

Click here for the Group Project Search. It’s located at the very bottom of the main FamilyTreeDNA page in the footer.

I’m going to use Estes as an example since I’m the volunteer administrator of that project and am very familiar with the lineages.

I’m searching for projects that include the surname Estes.

The projects displayed on the list are projects where the volunteer administrators listed Estes as a possible surname of interest. It doesn’t mean those projects will be of interest to everyone or every line with that surname, but evaluate each project listed.

You probably want the surname project, but if there’s not a surname project for your surname, try alternate spellings or consider checking other projects.

You can see at the bottom that 384 people of both sexes by the surname of Estes have tested at FamilyTreeDNA.

Now, let’s look at the Estes project. Note that not everyone with the Estes surname has joined the Estes project.

I’ve clicked on the “Estes” link which takes me to an additional information page where I can read a description and click to view the project.

For the Estes project, you do not have to join to view the results. Nor does your surname have to be Estes. All Estes descendants of any line are welcome. Everyone can benefit from the Advanced Matching within project feature to see who else you match within the project by selecting a wide range of individual and combined filters.

Click on the Project Website link shown in the search results.

If you’re searching for a male Estes ancestor, you’ll want to review the project’s Y-DNA Results and the Group Time Tree, for sure, and possibly the Map as well.

Let’s pretend I’m trying to determine if anyone has tested who descends from my ancestor, Abraham Estes, the founding Estes ancestor in Virginia who arrived in the mid-1600s.

In the Estes project, the volunteer administrator has divided the Estes male participants by sons of Abraham, the immigrant. Only three are shown here, but there are several.

Some of the participants have completed their Earliest Known Ancestor information, in the red box. Sometimes people don’t think to update these when they make breakthroughs.

If you descend from Abraham’s son, Sylvester, three men have taken the Big Y-700. That’s the test results you need.

If you descend from Abraham’s son, Abraham, no project participants have taken the Big-Y test to represent that line, although six people have tested, so that’s great news. Maybe you can offer an upgrade scholarship to one or some of those men.

In other words, to establish the haplogroup for that lineage, at least two men need to test or upgrade to the Big Y-700, preferably through two different sons of the common ancestor. A new, more defining haplogroup is often formed every two or three generations for Y-DNA.

Your genetic pedigree chart looks a lot like your genealogy pedigree chart.

Click any image to enlarge

The project Group Time Tree shows selected groups of men who have taken Big Y tests, along with their Earliest Known Ancestor, if they’ve provided the information. This is one of the reasons why the Big Y-700 is so critically important to genealogy. The time granularity is amazing and can answer the question of whether men by the same surname descend from the same common ancestor – and when.

If you’ve taken a Family Finder autosomal test at FamilyTreeDNA, or uploaded an autosomal file from another vendor, you may match one of these men or another male that descends from the Estes line if they, too, have taken an autosomal test.

This same process applies to mitochondrial DNA, but generally surname projects aren’t (as) relevant for mitochondrial DNA since the surname changes every generation. However, sometimes other projects, such as the Acadian AmerIndian Project are quite beneficial if you have Acadian ancestry, or a geographic or regional project like the French Heritage Project, or something like the American Indian Project.

Another great way to find testers is by utilizing your Family Finder test.

Step 4 – Family Finder at FamilyTreeDNA

The next step is to see if you match anyone with the surname you’re searching for by using your autosomal test results, so select your Family Finder Matches.

At FamilyTreeDNA you’ll want to search your matches by the surname you seek. This surname search lists any tester who has that surname, or anyone who has entered that surname in their surname list. Please note that this search does NOT read ancestors in your matches’ trees. You’ll still need to view trees.

Reviewing the 32 Estes Family Finder matches reveals several men, but one man with the Estes surname has already taken a Y-DNA 25-marker test, so he would be an excellent candidate to offer a Big Y-700 upgrade scholarship. If he’s not interested or doesn’t respond, there are several more men to contact.

Click on your match’s name to display the profile card, along with the Earliest Known Ancestors, both Y-DNA and mitochondrial DNA haplogroups if they have tested, and the assigned haplogroup based on their testing level.

Craft an email and offer a testing scholarship. This will help both of you. I’ll provide a sample email at the end of this article.

If you match a female with an Estes surname, her father, brother, uncle or cousin may either have already tested or be willing.

If you match someone who has a different surname, that means they have an Estes surname in their surname list and may know a potential tester. If your match has a tree, click to check.

I’ve found that matching through a company where you’ve both tested is the easiest way to encourage someone to take an additional test, but certainly, it’s not the only way.

Step 5 – WikiTree

WikiTree is a quick and easy way to see if anyone has taken Y-DNA or mitochondrial DNA test that should reflect a particular ancestor’s Y-DNA or mitochondrial DNA.

I just googled “Moses Estes 1711-1787 WikiTree” and clicked to view.

Each ancestor includes both Y-DNA and mitochondrial DNA information, in addition to people who descend from that ancestor through only autosomal lines.

In this case, two men have provided their Y-DNA results that pertain to Moses Estes. They have tested at different levels, which is why they have different haplogroups. That doesn’t mean either is “wrong,” one is just more refined than the other. You can correlate their kit number with the Estes surname project. People often don’t update their haplogroup information at WikiTree when it’s updated at FamilyTreeDNA.

Please note that if the genealogy is wrong, either at WikiTree or individually, the haplogroup may not reflect the appropriate lineage for the ancestor. Check to be sure that there’s no conflict showing between two testers for the same ancestor. For example, the same ancestor clearly can’t have two different base haplogroups, like E and R. The Discover Compare tool can help you evaluate if two haplogroups are in the same part of the Y-DNA tree.

When possible, it’s always best to test a close family member to represent your lineage even if someone else has already tested.

Scan down the list of autosomal testers for that ancestor to see if there’s someone with the Estes surname.

WikiTree provides additional tools to find descendants.

Sign in to WikiTree. You’ll see the ID of the profile you’re viewing – in this case – Estes-167. Click the down arrow and select “Descendants.”

This view shows all descendants through five generations, but you can click on DNA Descendants to see only Y-DNA descendants, X-DNA, or mitochondrial DNA descendants for female ancestors.

You may find people who are living and have added themselves who you can contact to offer a DNA testing scholarship.

Step 6 – MyHeritage

At MyHeritage, you can also search your DNA matches by surname.

Click on “Review DNA Match” to view more detail, including locations. Look to see if you have a Theory of Family Relativity Match which suggests how you may be related. That’s golden!

There’s no Y-DNA information at MyHeritage, BUT, you can search by surname and view DNA matches that either carry that surname or have that surname in their tree as an ancestor.

I have a total of 75 “Estes” matches, and other than the kits that I manage, searching through my matches shows:

  • Two Estes men connected to the same small tree, but that’s OK, I’m a genealogist!

  • One Estes male match with a Theory of Family Relativity. My lucky day!

You can contact your match easily through the MyHeritage messaging system and offer a DNA testing scholarship at FamilyTreeDNA. You may also want to share your email address.

MyHeritage customers may not be familiar with Y-DNA or mitochondrial DNA testing, so you might want to share this article about the 4 Kinds of DNA for Genealogy.

MyHeritage testers can also upload their DNA file to FamilyTreeDNA for free to receive autosomal matches plus a complimentary mid-range Y-DNA haplogroup. This free haplogroup is not even close to the detailed resolution of a Big Y-700 test, but it’s something, and it may well be an enticing first step for people who are only familiar with autosomal testing.

Step 7 – At Ancestry

At Ancestry, select DNA Matches and then search by surname.

You can search by the surname of the tester, which is very useful, or by people who have Estes in their trees.

I started with the surname Estes, because it’s the most straightforward and I may find a perfect male candidate for Y-DNA. If someone’s “screen name” doesn’t show as Estes, they won’t appear in the results of this search. In other words, if your Ancestry screen name is “robertaestes” you won’t show in this search, but “Roberta Estes” will.

For mitochondrial DNA, you would want to search for the surname in your matches’ trees. Unfortunately, you cannot search for the specific ancestor in someone’s tree, at least not directly.

Of my 19 Estes surname matches, ten are males, and of them:

  • Three have unlinked trees
  • Three have very small linked trees, but I can work on extending those if need be
  • Three have public linked trees AND a common ancestor, which means ThruLines

I can review which ancestor we share by clicking on my match’s name

The Estes side of this man’s tree has only one person and is marked “private,” but Ancestry has suggested common ancestors based on other people’s trees. (Yes, I know trees are dicey, but bear with me.)

It’s also worth mentioning that you can be related through multiple lines. I share surnames from Acadian lines with this man, but that really doesn’t matter here because I’m only using autosomal matching to find an Estes male.

Click on “View Relationship” to see our common Estes ancestor’s ThruLine.

The ThruLine shows how Ancestry thinks we’re related on the Estes line.

I can also click on “View ThruLines” to see all Thrulines for John R. Estes, which shows four additional males, some of which did NOT appear in the Estes surname search, and some of which don’t appear further up the tree. In other words, check all Estes ThruLine ancestor generations.

Don’t rely solely on Ancestry’s surname search.

Go directly to your ThruLines on the DNA menu.

Ancestry only reaches back seven generations, which for me is Moses Estes and Luremia Combs. Moses has 95 matches, but he has been given some incorrect children. Again, for this purpose, it doesn’t matter. Within all ThruLine matches, I found three Estes males who all descend through John R. Estes. Check every generation.

However, Luremia Combs shows promise for mitochondrial DNA descendants. Unfortunately, only two of her daughters are represented in ThruLines, and both of their descendants descend through Luremia’s grandsons. That’s too bad, because I need Luremia’s mitochondrial DNA line.

It’s easy to message your Ancestry matches. You may want to mention that they can upload their DNA file to FamilyTreeDNA for free where they will receive more matches and males will receive a complimentary mid-level Y-DNA haplogroup.

Please note that, in general, ThruLines need to be evaluated very carefully and are prone to errors, especially if you accept Ancestry’s suggestions of ancestors instead of carefully building out your own tree. Regardless, you can still find Estes cousin matches in your match list and by using ThruLines to find people that do not show up in an “Estes” match search.

Step 8 – At 23andMe

At 23andMe, you can search for anyone who either has the Estes surname or has included that surname in their “Family surnames” list. Keep in mind that your matches at 23andMe are restricted to either 1500 if you don’t have a subscripition, or about 4500 if you do have a subscription.

On my match list, I have two males with the Estes surname.

23andMe provides a mid-level Y-DNA haplogroup. You can’t use this to confirm the lineage when comparing with FamilyTreeDNA, especially given that 23andMe provides no genealogy or user-provided tree, but it is a clue.

Both Estes men at 23andMe have Y-DNA haplogroup R-CTS241. You could use this in some cases to potentially eliminate these matches at 23andMe. For example, if men in your lineage in the Estes project are in haplogroup R and your 23andMe matches are showing as haplogroup E, or any other base haplogroup, their common ancestor is tens of thousands of years ago.

Comparing the 23andMe haplogroup, which in this case is about 4500 years old, to contemporary testers who have taken the Big Y-700, which reaches within a few generations, isn’t terribly useful. These matches are extremely useful to identify individuals to reach out to for further information and potentially offer a Y-DNA testing scholarship at FamilyTreeDNA.

Remember, this also applies to females who have included Estes in their family surnames, given that they may have Estes male relatives.

By clicking to view your match, you can see if they have provided Family Background information, including a link to a family tree someplace.

Sometimes, there’s great information here, and other times, nothing.

You can’t verify this lineage without genealogy information.

I suggest leaving a genealogy-focused message, including where they can see your tree in addition to your Estes connection. Also include your e-mail.

You may want to say that if they descend appropriately, you have a Y-DNA or mitochondrial DNA testing scholarship, or you may want to wait to see how they descend. You can also ask if they have already taken a Y-DNA or mitochondrial DNA test at FamilyTreeDNA.

Step 9 – FamilySearch and Relatives at RootsTech

We’re getting ready for RootsTech 2025 which takes place in March. In the month or so before the last two RootsTechs, FamilySearch provided an absolutely wonderful tool called “Relatives at RootsTech.”

I’ve written about this several times, but essentially, you can see, by ancestor, other people who are registered both in-person and virtually for RootsTech, and how they descend.

Here’s an example.

In both years, I’ve found several people who descended from common ancestors AND were very willing to take the relevant DNA test. That’s a huge win-win for everyone.

The best part is that because these people have freshly registered for RootsTech, the reply rate is almost 100%.

I’ll write about this as soon as RootsTech makes it available this year. Fingers crossed that they do!

Step 10 – Social Media

Social media wouldn’t be my first choice to find DNA testers, but I have found perfectly willing cousins this way. You may be less successful on Facebook or other social media platforms, but if you’re striking out elsewhere, there’s absolutely no downside to trying.

You can enter a surname and search on Facebook, but I prefer to do a Google search like “Estes genealogy on Facebook” or even just “Estes genealogy,” which will produce far more widespread information, some of which may be irrelevant.

That Facebook Google search provided the names of two groups. People join groups because they have an interest, and I’ve had good luck in Facebook genealogy groups.

A Search of “Estes” on Facebook itself, then selecting “people” provided a list of Estes Facebook users.

I’ve had far better luck by joining a group that is focused on Estes genealogy, or even a county genealogy group that includes Estes families, than individuals. People who join any Estes group or project likely have an interest in that surname.

If you have a common surname, or there’s a park named after your surname, like Estes Park, you’ll probably want to focus by using Google searches for Estes genealogy.

The Descendants of Abraham Estes Facebook group has 222 members, of whom at least 31 are males with the Estes surname. Facebook just might be an underestimated resource.

If there isn’t a genealogy-focused group for your surname, you might want to consider starting one and encouraging people to join.

It can’t hurt, and it just might help. Before you start reaching out to random people on Facebook, please do a privacy checkup – I wrote about how, here.

Sale Prices

Remember, the sale prices at FamilyTreeDNA for new tests and upgrades last through year-end.

In my experience, it’s best to test as soon as someone agrees. You never know what will happen otherwise. I’ve had people pass away before they could swab. And yes, we’ve done funeral home swabs, too.

There’s no one-size-fits-all, but here’s a rough draft contact letter.

Potential Contact Letter

You’ll want to include several critical pieces of information.

Essentially:

  • Introduce yourself
  • Say their full name on their test AND the testing company in the title of an email. I manage many tests and if I receive an email that says, “Hi, can you tell me how we match” without telling me which person they match, I can’t even begin to answer.
  • Explain your genealogy connection
  • State your purpose in writing
  • Explain how a specific test will help them too
  • Offer to answer questions

Be sure to modify this letter to reflect your own voice and circumstances. You don’t want this to read like a form letter.

Dear cousin (insert their full name here,)

It was so nice to find our DNA match at <company name> (or we share a common ancestor, or appropriate circumstance.) (If you are managing someone else’s kit, say the name of who they match and explain that you manage their DNA kit.)

I descend from (ancestor plus birth and death date) who lived in Halifax County, Virginia and was married to (spouse.) You can view my tree at (insert link that does not require a subscription for viewing unless you match them on that platform. I use MyHeritage because everyone can view their trees)

I would very much like to confirm that our line descends from Abraham Estes (or relevant information meaning your reason for wanting them to test.)

Given that my surname is x (or I’m a female), we need to test the Y-DNA of a male who is descended from (ancestor) through all males to the current generation. (Or mitochondrial DNA descended through females to the current generation which can be male.)

FamilyTreeDNA provides this testing and shows who you match on that specific line using the Y chromosome (mitochondrial DNA).

This testing may connect us with earlier ancestors. Genetics can be used to determine when we share common Estes ancestors with others who test, where we come from overseas, and when. Even if we match ancient DNA samples that may tell us where our ancestors lived before surnames. In other words, where did we come from?

(Include a nice paragraph, but not a book about your ancestral lineage here.)

I have a DNA testing scholarship for someone from this line and you are the perfect candidate. I would like to take advantage of the current sales. If you’re interested, I only need two things from you.

First, permission so that I can order (or upgrade) and pay for the test, and second, an address where to send the test (unless it’s an upgrade). (If it’s an upgrade at FamilyTreeDNA, they can use a stored sample or will sent them a new kit if there’s not enough DNA.)

If you have any questions, please let me know. I’m very excited that we may be able to learn more about our heritage.

Please email me at xxx or call me at xxx if you have questions.

Your name

I know one person who offers to review results over Zoom. Someone else stresses that the tester’s email is attached to their test and they are always in control of their results. Another person asks them to join a project they manage to assure that they can follow their matches over time.

Customize this communication in your own voice and to fit the circumstances of each match.

It’s just me, but since I’m ordering while the tests are on sale, unless the person uploads their DNA file from another vendor, I add on a Family Finder test too and explain why. You never know if they will match you or another cousin, and they may have that match that eventually breaks down the next brick wall. Shared matches are powerful evidence and it’s a lot easier to add that test on now than try to contact them again later.

You Don’t Know What You Don’t Know

Which ancestors do you need Y-DNA or mitochondrial DNA results for? Methodically check each line.

There’s so much to learn. Don’t leave information on the table by virtue of omission.

Leave no stone unturned!

You don’t know what you don’t know.

Who’s waiting out there for you?

____________________________________________________________

Share the Love!

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If you haven’t already subscribed (it’s free,) you can receive an e-mail whenever I publish by clicking the “follow” button on the main blog page, here.

You Can Help Keep This Blog Free

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase your price but helps me keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Uploads

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Great News – Both e-Pub and Print Version of “The Complete Guide to FamilyTreeDNA” Now Available Worldwide  

  • Anyone, anyplace, can order the full-color, searchable, e-pub version of The Complete Guide to FamilyTreeDNA – Y-DNA, Mitochondrial, Autosomal and X-DNA from the publisher, Genealogical.com, here.
  • Customers within the US can order the black and white print book from the publisher, here.
  • Customers outside the US can order the print book from their country’s Amazon website. The publisher does not ship print books outside the US due to customs, shipping costs, and associated delays. They arranged to have the book printed by an international printer so that it can be shipped directly to Amazon for order fulfillment without international customers incurring additional expenses and delays. If you ordered the book previously from Amazon and a long delivery time was projected, that should be resolved now and your book should be arriving soon.

Comprehensive

This book is truly comprehensive and includes:

  • 247 pages
  • More than 267 images
  • 288 footnotes
  • 12 charts
  • 68 tips
  • Plus, an 18-page glossary

To view the table of contents, click here. To order, click here.

Thank you, everyone, for your patience and your support.

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Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an e-mail whenever I publish by clicking the “follow” button on the main blog page, here.

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I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase your price but helps me keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

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Complete Guide to FamilyTreeDNA Released in Hardcopy

Just what many of you have been waiting for! The hardcopy print version of the Complete Guide to FamilyTreeDNA has just been released.

As shown in the table of contents below, The Complete Guide to FamilyTreeDNA contains lots of logically organized information! It includes basic education about genetic genealogy and how it works, instructions on using the FamilyTreeDNA tests and tools, plus an extensive glossary.

Enjoy!

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Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an e-mail whenever I publish by clicking the “follow” button on the main blog page, here.

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I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase your price but helps me keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

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Announcing: The Complete Guide to FamilyTreeDNA; Y-DNA, Mitochondrial, Autosomal and X-DNA

I’m so very pleased to announce the publication of my new book, The Complete Guide to FamilyTreeDNA – Y-DNA, Mitochondrial, Autosomal and X-DNA.

For the first time, the publisher, Genealogical.com, is making the full-color, searchable e-book version available before the hardcopy print version, here. The e-book version can be read using your favorite e-book reader such as Kindle or iBooks.

Update: The hardcopy version was released at the end of May and is available from the publisher in the US and from Amazon internationally.

This book is about more than how to use the FamilyTreeDNA products and interpreting their genealogical meaning, it’s also a primer on the four different types of DNA used for genealogy and how they work:

  • Autosomal DNA
  • Mitochondrial DNA
  • Y-DNA
  • X-DNA

There’s a LOT here, as shown by the table of contents, below

This book is chocked full of great information in one place. As an added bonus, the DNA glossary is 18 pages long.

I really hope you enjoy my new book, in whatever format you prefer.

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Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an e-mail whenever I publish by clicking the “follow” button on the main blog page, here.

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I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase your price but helps me keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

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Why Don’t Our Y-DNA Haplogroups Match?

I’ve been asked this question several times recently, and the answer is resoundingly, “it depends.” There are several reasons why Y-DNA haplogroups might not match and most of them aren’t “bad.”

How Haplogroups Work

Haplogroups are the 79,000+ branches of the Y-DNA phylogenetic tree which you can view here, along with countries where those haplogroups are found. You can think of haplogroups as genetic clans of either closely or distantly related men. Major haplogroup branches have unique letters assigned. Downstream or younger haplogroups are designated by a letter-number sequence that is always preceded by the main haplogroup letter.

Image courtesy FamilyTreeDNA

Major haplogroups were formed tens of thousands of years ago, with more recent haplogroups added as they’ve been discovered. Haplogroups are discovered and added every day thanks to the Big Y-700 test. You can read more about that process, here.

As you look at the pie chart above, you’ll notice that haplogroup R represents about half the men who have tested and has several major subbranches. Every haplogroup R man belongs to all of the branches above his own that lead back to the root of haplogroup R.

Using haplogroup R, which is R-M207, its identifying SNP, as an example, it immediately splits into two branches: R-M173, which has 37,000+ more branches, and R-M479, which has 313 branches. My Estes men fall into a haplogroup several steps beneath R-M173, but they are still members of haplogroups R-M173 and R-M207, even though their descendant haplogroup is R-BY490, which was formed by a mutation that occurred 20,000 years later.

Haplogroup R-M173, then, in turn, leads back to Y-Adam, the first man to have lived and has descendants today.

As we approach the question of why haplogroups of two men might differ, we will review tools to use and how to interpret your findings to reach the appropriate answer for your situation.

What is Your Goal?

You may be looking for a very specific answer, or this may be a more general question.

  • If you’re evaluating closely related men who have different haplogroup assignments, not matching can be very disconcerting. Breathe. There are several perfectly legitimate reasons why they may not match, and we have easy, free analysis tools.
  • If you’re looking at your Y-DNA match list at FamilyTreeDNA, you may or may not match other men closely, but you do “match” at some level if they are on your match list. You may see several different haplogroups in your match list. How closely you match those men is a different question.
  • If you’re looking at autosomal results at FamilyTreeDNA, you may see haplogroups listed for males. You may or may not “match” the haplogroup of men with the same surname. What does this mean, and why don’t you match? Your autosomal match may have nothing to do with your paternal line, or it may be because of your paternal line.

We will cover all of these scenarios.

Where Did You Both Test?

  • Are you comparing apples and apples?
  • Did you both test at the same company?
  • Did you both take the same type or level of test?

These factors all make a difference.

Which Test Did You Take?

There are four types of tests that will provide males with some level of Y-DNA haplogroup.

Autosomal Tests – Some companies include a few Y-DNA location probes in their autosomal test, meaning that they test a few haplogroup-specific Y-DNA locations. LivingDNA, 23andMe, and FamilyTreeDNA’s Family Finder test provide a mid-level Y-DNA haplogroup to customers. The haplogroup that can be determined from these tests depends on a variety of factors, including the vendor, the probes they selected for their chip, the test version, and if that location is successfully read in the test.

Note that FamilyTreeDNA supports autosomal uploads from MyHeritage and Ancestry who do not provide Y-DNA haplogroups to customers, but who do test some Y-DNA locations. Therefore you can upload your autosomal test from those companies to FamilyTreeDNA for free and receive at least a cursory Y-DNA haplogroup.

FamilyTreeDNA is currently processing all of its Family Finder tests, followed by tests uploaded from other vendors, to provide all genetic male testers with a Y-DNA haplogroup at some level. Different vendors and test versions test different Y-DNA SNPs, so your mileage may vary. Y-DNA haplogroups are a free benefit at FamilyTreeDNA.

STR Tests – At FamilyTreeDNA, you can purchase both Y-37 and Y-111 STR (short tandem repeat) Y-DNA tests that provide matching at the number of locations you purchased, plus a predicted haplogroup based on those results. These haplogroup predictions are accurate but are often relatively far back in time.

If you match someone on STR tests, your match may be very recent or before the advent of surnames. For a more specific haplogroup, you need to purchase the Big Y-700 test, which provides at least 700 STR match locations but, more importantly, sequences the entire gold-standard region of the Y-chromosome for the most precise haplogroup and matching possible.

  • When viewing matches of two men who ONLY took STR tests, STR marker matches are more important for genealogy than haplogroups because the haplogroups were formed thousands of years ago.
  • When viewing matches on the Big Y-700 test, haplogroup matching is much more specific and reliable than STR matches because the mutations (SNPs – single nucleotide polymorphisms) that form haplogroups are much more stable than STRs which mutate unpredictably, including back mutations.

SNP Confirmation Tests – Historically, FamilyTreeDNA customers could purchase individual SNPs to confirm a haplogroup, or SNP packs or bundles to do the same for a group of SNPs. With the advent of both the Family Finder haplogroup assignments, and the Big Y-700, these individual tests are no longer necessary or advantageous and are being discontinued.

Big Y-700 Test – At FamilyTreeDNA, the Big Y-700 test provides the most granular and specific haplogroup possible, most often well within a genealogical timeframe. You may be able to tell, based on previously undiscovered mutations, that two people are brothers or father and son, or, depending on who else has tested and when mutations formed, testers may match further back in time. Here’s an example of using the results from multiple testers in the Estes DNA Surname Project.

You can also match men who took the Big Y-500 test which is less specific than the Big Y-700. In the now-obsolete Big Y-500 test, a smaller portion of the Y chromosome was sequenced and testers only received about 500 STR locations. The Big Y-700 test has been enriched to provide a wider range of more specific information. Men who originally took the Big Y-500, then upgraded to the Big Y-700, will very probably have a new haplogroup assignment based on the expanded coverage and increased resolution of the Big Y-700 test. The Big Y-700 ferrets out lineages that the Big Y-500 simply could not, and continues to provide additional value as more men test, which facilitates the formation of new haplogroups.

What Do You Mean by Match?

Matching doesn’t mean you have to have the exact same haplogroup. A perfectly valid match can have a different haplogroup because one haplogroup is more specific or refined than the other. Matching exactly as a result of a predicted STR haplogroup is much less useful than matching closely on a much more recent Big Y-700 haplogroup.

Not all haplogroups are created equal.

I know this is a bit confusing, so let’s look at real-life examples to clarify.

STR to STR or Autosomal to Autosomal Haplogroup Match

Two males might match exactly on a mid-range Family Finder autosomal haplogroup or on a STR-predicted haplogroup like R-M269, which is about 6350 years old.

This haplogroup “match,” even though it might be exact, does not confirm a close match and really only serves to eliminate some other haplogroups and confirm that a closer match is possible. For example, R-M269 men don’t match someone in haplogroup J or E. You may or may not share a surname. You may or may not still “match” if you both upgrade to the Big Y-700.

In this case, a father/son pair would match exactly, as would two men with different surnames whose common ancestor lived 6000 years ago.

Note that if you’re comparing autosomal-derived haplogroups across different vendor platforms, or even different DNA testing chip versions on the same platform, you may see two different haplogroups. Different vendors test different locations. Please note that second cousins and closer will always match on autosomal DNA, but relationships further back than that may not. Y-DNA very reliably reaches far beyond the capabilities of autosomal DNA due to the fact that it is never mixed with the DNA of the other parent – so it never divides or is watered down in time. When comparing two autosomally-generated haplogroups of men who are supposed to be closely related, always check their autosomal match results too.

Use the free Discover Tool to find various categories of information about any haplogroup, including its age. Take a look at R-M269 here.

Using Discover to Compare Haplogroups

You can always use the Discover tool to compare two haplogroups.

Go to Discover (or click through if you’re signed on to your FamilyTreeDNA Y-DNA page), then enter the first haplogroup you’d like to compare.

Click search to view information about that haplogroup.

On the menu bar, at left, click on Compare.

Add the second haplogroup.

I’m selecting E-M35, a completely different branch of the phylogenetic tree.

R-M269 was formed about 6350 years ago, while E-M35 was formed about 25,000 years ago. Their common ancestor was formed about 65,000 years ago. Clearly, these two paternal lineages are not related in anything close to a genealogical timeframe.

These two men would never match on an STR test, but could easily match on an autosomal test on any line OTHER than their direct paternal line.

Now let’s compare two haplogroups that are more closely related.

Haplogroup R-M222 is very common in Ireland, so let’s see how closely related it is to R-M269 which is very common in western Europe.

We see that R-M222 descends from R-M269, so there is no “other haplogroup” involved.

R-M222 was formed about 2100 years ago, around 4250 years after R-M269 was formed.

There are 17 steps between R-M222 and R-M269.

The bottom block shows the lineage from R-M269 back to Y-Adam.

How cool is this??!!

Big Y-700 to Autosomal or STR Haplogroup Comparison

Joe took the Big Y-700 test and discovered that he’s haplogroup R-BY177080.

Joe noticed that his son, who had initially taken an STR test, had been assigned haplogroup R-M269. Then, his son took a Family Finder test and his haplogroup changed to R-FGC8601.

Joe was confused about why he and his son’s haplogroups didn’t match.

First, let’s check Family Finder to confirm the parent/child relationship. Joe’s son is clearly his son.

So why doesn’t Joe’s son’s haplogroup match Joe’s haplogroup? And why did Joe’s son’s haplogroup change?

Joe’s son had not taken a Big Y-700 DNA test, so Joe’s son’s R-M269 haplogroup was initially predicted from his STR test.

Joe’s son’s updated haplogroup, R-FGC8601 was generated by the Family Finder test. Think of this as a bonus. If you’re a male and haven’t yet, you’ll soon receive an email telling you that you’ve received a Family Finder Y-DNA haplogroup. It’s your lucky day!

Family Finder haplogroups always replace STR predicted haplogroups since they are always more specific than predicted STR haplogroups. Big Y-700 haplogroups always replace STR-generated haplogroup predictions and Family Finder haplogroups because they are the most specific.

Let’s compare these results using Discover.

Joe’s son’s original predicted haplogroup was R-M269.

Discover Compare shows us that Joe’s Big Y-700 Haplogroup, R-BY177080, is a descendant of R-M269.

So, they actually do “match,” just several branches further up the tree

Joe’s son’s more precise Family Finder haplogroup was assigned as R-FGC8601.

Discover Compare shows us that Joe’s Big Y-700 haplogroup also descends from R-FGC8601.

You can see that the haplogroup generated by Family Finder is more precise by about 4700 years and improves that comparison.

R-M269 was formed about 6350 years ago, but R-FGC8601 was formed about 1700 years ago.

Joe’s Big Y-700 haplogroup, R-BY177080 was formed about the year 1900, improving the family haplogroup by another 1600 years or so.

Joe’s son’s Family Finder haplogroup moved down the haplotree 21 branches and 4650 years, for free! If Joe’s son were to upgrade to the Big Y-700, they might very well be assigned a new haplogroup that, for the time being, only they share.

Of course, Family Finder doesn’t provide Y-DNA matching so you still need the Y-DNA tests for that important aspect of genealogy.

Big Y to Big Y Comparison

In our next example, a group of men, including a father and son or other very close relative may take the Big Y-700 test and have different haplogroups. If you’re saying, “Whoa Nelly,” hear me out.

George took a Big Y-700 test and discovered that he is haplogroup R-FGC43597. His son and grandsons tested, and they are haplogroup R-FTC50269. What happened? Shouldn’t they all match George?

On George’s Big Y-700 block tree, you can see that a mutation, R-FTC50269, occurred between George and his son. George doesn’t have it, but his son does.

A haplogroup isn’t “named” until there are two men with the same mutation in the same lineage. Therefore, when George’s son initially tested, he would have been assigned to the same haplogroup as George, R-FGC43697, but with one extra variant, or mutation.

Of course, that extra mutation was passed from George’s son to both of his grandsons, so when the first grandson tested, the new haplogroup, R-FTC50269 was assigned as a result of that mutation. Now, George has one haplogroup and his son and grandsons have a different haplogroup, one branch downstream.

Using Discover to check the haplogroup ages and path, we find that indeed, these haplogroups are only one step apart.

Checking Family Finder results can always verify that the match is close or as close as you expected.

Haplogroup Assignments

Haplogroup assignments range from good to better to best.

Good Better Best
STR predicted Yes – but further back in time
SNP Packs (now obsolete) Between good and better
Family Finder autosomal Yes – generally midrange between STR predicted and the Big Y-700
Big Y-500 (need to upgrade) Usually between better and best
Big Y-700 The best – usually within a genealogically relevant timeframe unless your DNA is rare

Where Are You?

Older haplogroups, such as the STR-predicted haplogroups are useful for:

  • Eliminating some potential matches
  • Identifying where that haplogroup originated at that specific point in time. In other words, where your ancestor lived when that haplogroup was born.

If your Y-DNA matches another Y-DNA tester at FamilyTreeDNA, your haplogroups will fall someplace on the same haplogroup branch, although they may be thousands of years apart. STR-predicted haplogroups are “older,” meaning they range in age from about 6500 years to tens of thousands of years ago. They can tell you where the haplogroup originated at that time.

Autosomal haplogroups will be newer, or more recent, than STR-predicted haplogroups, but still (sometimes significantly) older than the Big Y-700 haplogroups..

FamilyTreeDNA provides Y-DNA haplogroups for free for every biological male who either takes the FamilyTreeDNA Family Finder test or uploads an autosomal result from either Ancestry or MyHeritage. Soon, 23andMe uploads will be resumed as well. This means that you will be able to view other men with a similar surname in your Family Finder results and:

  • Rule them out as a paternal line match.
  • Check your STR matches if they have taken a Y-DNA test
  • Check your Big Y-700 test for matches if both men have taken a Big Y test.
  • Encourage your matches to take a Big Y-700 test so you can see how closely you match on your paternal line.
  • Use the Discover Compare and other tools to reveal more information.

Family Finder haplogroups are relatively new, so currently, all new Family Finder testers are receiving haplogroups. Older Family Finder tests are being processed and will be followed by autosomal tests uploaded from other vendors. Haplogroups from autosomal tests are confirmed and will be newer, or more recent, than STR-predicted haplogroups.

The only test that can bring your haplogroup to current, meaning the most refined, recent, personal haplogroup, is the Big Y-700 test. Without taking the Big Y-700 test, you’ll forever be stuck with an older, less informative haplogroup branch. The Big Y-700 allows us to reliably sort families into lineages based on branching mutations.

The Big Y-700 haplogroup is:

  • The most detailed and granular possible.
  • Determined by sequencing the Y chromosome.
  • A test of discovery that continues to provide additional value as more men test and new haplogroups are formed.

Big Y-700 haplogroups generally fall into a genealogically useful timeframe and can be very recent.

The Discover tool and Time Tree provide a wealth of information about your ancestors, including locations, migration paths, ancient DNA, and more.

You Don’t Know What You Don’t Know

Now that you understand how to compare and interpret haplogroup matches, what additional information can you learn?

I always encourage Y-DNA matches to upgrade to the Big Y-700. Why? You don’t know what you don’t know. The article, Bennett Greenspan: Meet My Extended Family & Discover Extraordinary Deep Heritage illustrates the benefits of the Big Y-700 for all matches. Upgrading 12-marker matches is exactly how he made his big breakthrough.

The Big Y-700 test answers many questions beyond simply matching by using Discover and the Group Time Tree.

  • Where were your ancestors?
  • Who do you match, and who were their ancestors?
  • Genetically and genealogically, how do your surname matches fit together?
  • Where were your matches’ ancestors, and when?
  • Which ancient DNA results do you match, and where were they located?
  • What is the history of locations where your ancestors were found along their journey?
  • How closely or distantly are you related to other Big Y-700 matches?
  • Can your matches’ information break down your paternal line brick wall, or at least move it back a few generations?

Where are your Y-DNA results along the spectrum of useful haplogroup information? Do you or your matches need to upgrade? Click here to upgrade or order a Big Y-700 test.

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Bennett Greenspan: Meet My Extended Family & Discover Extraordinary Deep Heritage

“My ancestors are in my soul. I can’t get them out of my mind.”

Bennett Greenspan

“And yes, I brake for cemeteries.”

Bennett Greenspan gave an incredibly interesting presentation at the 15th International Genetic Genealogy Conference held by FamilyTreeDNA in November 2023. Since his retirement in January 2021, he has been able to focus on his genealogy. Once a genealogist, always a genealogist.

Bennett said some things I hadn’t thought about, and now I’m viewing Y-DNA matches with a different perspective – based on how he’s using his results.

Ever since I met him, Bennett’s focus has been to use genetics to unravel his complex Jewish heritage.

The questions that drive Bennett are the same ones that motivate most genealogists:

  1. Who are we?
  2. Where did we come from?
  3. Where were we before we were there?
  4. How did my ancestors get there?

Bennett “lost his family lines” before the mid-1800s due to his Jewish heritage, exacerbated in the 1930s by the devastation wrought by the Holocaust. Families were either killed or scattered to survive. It has been through Y-DNA in particular that he has been able to establish unquestionable and confirmed connections with other Greenspan men, sometimes by similar but different surnames, like Green, and sometimes with other surnames entirely.

When Bennett first started down this path, he tested more than 62 men before actually finding one a decade later that matched his Y-DNA. Bennet commented that it was “a little frustrating.”

Persistence is the key, and sometimes, genealogy is a waiting game, but that’s small comfort to genealogists during that unproductive waiting period.

Eventually, Bennett reassembled his family, at least somewhat, but it was a long journey. Here’s Bennett’s incredible story, including surprises, as he tells it.

Bennett discovered genealogy at age 12 and, like many genealogists, created a pedigree chart by talking to his family.

I love the mark-outs. How many of us still have our first chart with its edits?

This is the young Bennett Greenspan, whose interest in genealogy would one day unlock secrets for all of us!

It was a long way from a decade with no matches to finding his genetic kin in Ukraine.

The Big Y-700 Time Tree shows Bennett’s lineage in Ukraine, but stepping back in time, some descendants of his ancestors are found in adjacent locations.

Bennett was passionately discussing his matches on the time tree and in the Greenspan project, so I visited the Greenspan DNA Project, where the earliest known ancestors of Bennett’s Big Y matches are shown on the Group Time Tree.

Bennett’s closest matches are shown as descendants of haplogroup J-ZS1718. He has additional matches who are not in the Greenspan project. Since this is the Group Time Tree, it only displays the people in that project, along with their earliest known ancestors, Isaac and Usher Greenspan.

12-Marker Matches

Bennett never fails to amaze me. He said something very important and profound about 12-marker matches that I really hadn’t thought about – at least not this way.

As a community, we are often guilty of discounting 12-marker matches, those that don’t match us at 25-markers or above, or with different surnames, as “too far back in time” or otherwise irrelevant. I always look at the names and earliest known ancestors of 12-marker matches, because that person may have tested back in the day when fewer markers were available. But if I don’t recognize something, I move on.

However, Bennett said that, ”Y-12 matches reach back to a common ancestor. 12-marker matches are not a quirk. They are related to you, just further back in time. You share a common ancestor with them, someplace. They may be more distant, but they are still your close matches.”

I’ve been in too much of a hurry for a quick win, and ignoring the (apparently not so) obvious.

Determining when and where their ancestors lived also paves the way to discover yours. Your Y-DNA and theirs were in the same place at the same time.

Of Bennett’s 171 12-marker matches, 107 have upgraded to the Big Y, probably mostly due to his encouragement. This benefits both them and Bennett by fleshing out the history of that entire group of men, including how they got to where they are found in the first available records. The Time Tree shows when Big Y testers shared a common ancestor, and based on Earliest Known Ancestor (EKA) locations, where. This provides further information about the lives of ancestors before contemporary records – in other words – people that we can never identify by name. It’s a window into ancestors before surnames.

Bennett notes that testers need to know their ancestral village or location to be most useful within the project, and of course, they need to enter their EKA information. Location information is how the Migration Map, Matches Map, and Discover tools, including the Time Tree, are built.

What Happened in Spain?

Bennett’s ancestors and those of his 12-marker matches are found in Spain, and as Bennett says, “One son stayed and one left about the year 296.”

While we have no idea of their names, based on the Time Tree combined with the cluster of earliest known ancestors, we know that they were in Spain, and when.

Their family story is revealed in the bifurcation of the tree found beneath haplogroup J-L823, formed about 296 CE. One line stayed in Spain, and Bennett’s line migrated to eastern Europe where that man’s descendants, including Bennett’s family, are found in the Russian Federation, Belarus, Poland, Lithuania, Sweden, Slovakia, Ukraine, Germany, Romania, the Czech Republic, and other eastern European locations. The closer to you in the tree and in time, the more relevant to your more recent ancestral story.

However, Bennett’s deeper ancestry, the migration of his ancestors to Spain, was only revealed by testing those more distantly related men. Those same men could well have been ignored entirely because they only matched at 12 markers.

According to Bennett, “Y-12 markers are important because these are the men most closely related to you in a database of 1 million men.”

How incredibly profound. How much have I been cavalierly overlooking?

How does this actually apply to Bennett’s results?

Bennett’s Spanish Matches

Bennett has the following STR panel matches who indicate that their EKA are from Spain. You can see that they match Bennett on a variety of panels.

  • X = yes, match
  • No = no match
  • Blank = not tested at that level.

In the Big Y GD column, the genetic distance (GD) is displayed as 15/660 where 15 is the number of mismatches, or the cumulative genetic distance ABOVE the 111 panel, and 660 is the number of STR markers above 111 with results.

The Big Y-500 test guaranteed a minimum of 500 total STR markers, and the Big Y-700 guarantees a minimum of 700 total STR markers, plus multiple scans of the balance of the Y chromosome for SNP mutations that define haplogroups. Testers don’t receive the same number of markers because the scan technology sometimes doesn’t read a specific location.

Tester 12 25 37 67 111 Big Y Test Big Y GD Big Y Match Haplogroup
AA X X X No No Yes 15/660 No J-FTD8826
DT X X No No X Yes 17/664 No J-FTE50318
JG X X No No
AR No No X X No No
ELR X X X No No
EL X X Yes 17/666 No J-FTE50318
GC X X X X No No
JC X No No
JLG X X No No No Yes 14/662 No J-FTE23540
MF X X No X No Yes 15/665 No J-FTD91126
MT X X X X No No
BE X X X X X Yes 20/664 No J-BY1795
DR X X X X X Yes 16/660 No J-FTC87344
EC X X X X X Yes 15/665 No J-FTC87344
GM X X No No No Yes 16/650 No J-FTD28153
GM X X X X No Yes 17/664 No J-FTD11019
LS X X No No No Yes 18/666 No J-FTD28153
NE X X X X X Yes 23/597 No J-BY1795
NC X No No
RR X X X No X Yes 22/659 No J-BY1795
TT X X X X X Yes 16/647 No J-FTC87344
XG X X X No No Yes 17/523 No J-BY167283
JA X X No No No Yes 15/646 No J-FTD11019

Of those 23 Spanish matches, sixteen have upgraded to Big Y tests, 14 of which are Big Y-700s, resulting in nine different haplogroups, all of which are descendants of Haplogroup J-L823. How cool is that?

The “Nos” in the Big Y Match Column aren’t mistakes. That’s right – none of these men match Bennett on the Big Y test, meaning they had more than a 30 mutation difference between them and Bennett on the Big Y test.

At first glance, you’d think that Bennett would have been disappointed, but that’s not the case at all! In fact, it was the information provided by these distant Spanish matches that provided Bennett with the information that his line had split sometime around the year 296 CE, with one branch remaining in Spain and his branch migrating to Eastern Europe, where he has lots of matches.

DNA Plus History

What was happening in Spain or the Iberian peninsula that involved the Jewish people about that time? Historical records exist of Jews living in that region before the fall of the Second Temple in about 70 CE, including records of Jews being expelled from Rome in 139 for their “corrupting influence.”

Furthermore, the Ancient DNA Connections for haplogroup J-L823, the most recent common ancestor (MRCA) for all of those branches, includes connections to multiple burials from:

  • Lebanon
  • Iran
  • Rome (from 1-400 CE)
  • Turkey
  • Jordan

Clearly, Bennett’s ancestor was in the Iberian peninsula around or before 296 CE. One branch stayed, winding up in Spain, and one headed for Europe.

Without these matches, some who didn’t match above the 12 or 25 marker level, how would Bennett have EVER known that his Jewish ancestors left the Middle East for Spain in the early years? How would he have known they migrated from Spain to Eastern Europe, and how would he have known that his line did not migrate directly from the Levant to Eastern Europe in the 9th century?

Big Y matches are typically within about 1500 years, but non-matches are still INCREDIBLY valuable. Without them, you can’t completely assemble your family story.

I noticed on the Time Tree that in Bennett’s Eastern European line, one of his ancestor’s brother lineages includes the Katzenellenbogen Rabbinic Lineage derived from ancient DNA samples.

Bennett’s successes have resulted from contacting his matches and encouraging upgrades. So how did he do it? What’s the magic sauce?

Contacting Matches

How to contact matches successfully is a question I see often. In fact, FamilyTreeDNA recently wrote about that in an article, here.

Bennett’s methodology for contacting his matches to encourage an upgrade is that he sends an email explaining why he’s encouraging them to upgrade, followed by a 2nd email three days later.

Bennett tells the recipient that we are at an inflection point in time. “It’s winter, the wind is blowing hard, and many of the leaves are gone.”

In other words, we need to cast the net wider and capture what we can, while we can. Unfortunately, many early testers have died, and with them, chapters of history are perishing.

Collaboration is key. In addition to encouraging upgrades, Bennett also offers Zoom calls to these groups of men to explain the results if they are interested.

What a GREAT idea! I need to begin offering that as well.

Upgrade Request

Bennett reaches out to his matches at various levels, but he expects his closer STR matches, meaning at the 67 and 111 marker level with the fewest mismatches, to match him on a Big Y-700 test and connect someplace between 300-600 years ago, which helps everyone flesh out their tree.

Bennett’s email:

Hello <name>,

Since you have already made a sizable investment in your Y-DNA, you now know that we come from the dominant male Middle Eastern group (Haplogroup J) of men who <subject here>.

What’s really neat is that our Y-DNA has recently been found in an archaeological site in Northwestern Jordan dated to about 4200 years ago. I know this because I upgraded to the Big Y, which tests SNPs, looking at several million locations on the Y chromosome of each man.

One academic customer recently compared this new technology as the difference between looking into space with binoculars versus the Hubble Telescope.

I don’t know if you are familiar with your list of matches at the highest level you’ve tested for, either Y-67 or Y-111. If you are, you should recognize my name and the names of others who have taken the Big Y test.

You’ll see what you’ll gain by letting me upgrade your test for you and determining whether you are related to my line – probably between about 200 years and 500 years.

This might be the second time that I have written to you on this matter; can I presume if I don’t hear from you that you’re not really interested in the Y-DNA subject anymore?

Can I run the test so that I can see how closely we are related – at my expense? (Of course, you get to see how closely related we are, too).

Please reply to me and say “yes.” You don’t even have to put a 🙂 if you don’t want to.

I started this company and this industry over 20 years ago. I predict that you will be happy with the history of YOU that this upgrade will uncover.

Best,

Bennett Greenspan

As you can see, this email can easily be personalized further and adapted to matches at the 37, 25, and 12 marker levels – or even Family Finder matches, now that intermediate-range haplogroups are being reported.

What’s Next?

I’m going back to every one of the kits I sponsored or that represent descendants of one of my ancestors to review their matches again – focusing not just on the closest matches with common surnames, but also on locations – and specifically at lower matching levels. I’ll also be checking their Family Finder matches for male surname matches, or similar surnames.

As is evident from Bennett’s tests, an entire mine of diamonds is out there, just waiting to be unearthed by a Big Y test.

And to think that some people have been advising people to ignore 12-marker matches out-of-hand because they are “entirely irrelevant.” They aren’t – for two reasons.

  1. First, some early testers only tested to that level
  2. Second, because of the deeper history that Big Y tests from those matches will uncover

You can view your Y-DNA matches, upgrade your own Y-DNA test, or order a Big Y-700 test if you haven’t yet tested by clicking here. What’s your next step?

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FamilyTreeDNA Provides Y DNA Haplogroups from Family Finder Autosomal Tests

Big News! FamilyTreeDNA is delivering holiday gifts early!

Y DNA haplogroups are beginning to be delivered as a free benefit to men who took the Family Finder test at FamilyTreeDNA. This is the first wave of a staggered rollout. Haplogroup results will be delivered to several thousand people at a time, in batches, beginning today.

This is no trivial gift and includes LOTS of information that can be used in various ways for your genealogy. Please feel free to share this article. The new Family Finder haplogroups are another reason to take a Family Finder test and to encourage other family members to do so as well.

How is this Even Possible?

Clearly, Y DNA is not autosomal DNA, so how is it possible to obtain a Y DNA or mitochondrial DNA haplogroup from an autosomal test? Great question!

Many autosomal DNA processing chips include a limited number of targeted Y and mitochondrial DNA SNP locations. Generally, those locations are haplogroup predictive, which is how haplogroup information can be obtained from an autosomal DNA test.

Compared to the actual Y DNA and mitochondrial DNA tests, only a small fraction of the information is available through autosomal tests. Only the full sequence mitochondrial DNA test or the Big Y-700 test will provide you with the full story, including your most refined haplogroup, additional information, and matching with other customers.

Having said that, haplogroups obtained from Family Finder provide important clues and genealogical information that will hopefully whet recipients’ appetites for learning even more.

Delivery Schedule

This first group of men to receive haplogroup results consists of testers who have purchased the Family Finder test since March 2019 when the most recent chip was put into production.

FamilyTreeDNA will be rolling haplogroups out in batches of a few thousand each day until everyone’s is complete, in the following order:

  • Family Finder tests purchased since March 2019 (their V3 chip)
  • Family Finder tests purchased between the fall of 2015 to March 2019 (their V2 chip)
  • Family Finder tests purchased from 2010 to the fall of 2015 (their V1 chip)
  • Autosomal uploads from other vendors for customers who have unlocked the advanced Family Finder features for $19

Uploaded DNA Files from Other Vendors

After the results are available for all males who have tested at FamilyTreeDNA, haplogroups will begin to be rolled out to customers who uploaded autosomal DNA files from other companies, meaning MyHeritage, Vitagene, 23andMe, and Ancestry.

To receive haplogroups for files uploaded from other vendors, the Family Finder advanced tool unlock must have been (or can be) purchased for $19. In addition to haplogroups, the unlock also provides access to the chromosome browser, myOrigins (ethnicity), Chromosome painting for myOrigins ethnicity, and ancient Origins.

Both MyHeritage and Vitagene tests are performed in the Gene by Gene lab. Those “uploads” are actually a secure business-to-business transaction, so the file integrity is assured.

Ancestry and 23andMe DNA files are downloaded from those companies, then uploaded to FamilyTreeDNA. Some people build “composite” files in the format of these companies, so FamilyTreeDNA has no way to assure that the original DNA upload file hasn’t been modified and it is a legitimate, unmodified, file from either 23andMe or Ancestry. Hence, in some situations, they are treated differently.

Both Ancestry and 23andMe utilize different chips than FamilyTreeDNA, covering different SNPs. Those results may vary slightly from results available from native FamilyTreeDNA tests, and will also vary from each other. In other words, there’s no consistency, and therefore haplogroup accuracy cannot be confirmed.

Haplogroups resulting from tests performed in the FamilyTreeDNA lab will be visible to matches and on the SNP pages within projects. They will also be used in both Discover and the haplotree statistics. This includes Family Finder plus MyHeritage and Vitagene DNA file uploads.

Tests performed elsewhere will receive haplogroups that will only be visible to the user, or a group administrator viewing a kit within a project. They will not be visible to matches, used in trees or for statistics.

At their recent conference, FamilyTreeDNA provided this slide during an update about what to expect from Family Finder haplogroups.

Today, only Y DNA haplogroups are being provided, but after the new mitochondrial tree is available, customer haplogroups are updated, and MitoDiscover (my name, not theirs) is released, FamilyTreeDNA is planning to provide mitochondrial DNA haplogroups for Family Finder customers as well. The current haplogroup estimate is late 2024 or even into 2025.

Unfortunately, some of Ancestry’s DNA files don’t include mitochondrial DNA SNPs, so some customers who’ve uploaded Ancestry files won’t receive mitochondrial haplogroups.

STR Haplogroups to be Updated

All FamilyTreeDNA customers who have taken Y DNA STR tests, meaning 12, 25, 37, 67, or 111 markers, receive predicted haplogroups. Often, the Family Finder extracted results can provide a more refined haplogroup.

When that is possible, STR test predicted haplogroup results will be updated to the more refined Family Finder haplogroup.

Furthermore, while STR results are quite reliably predicted, Family Finder results are SNP-confirmed.

Notification

When your Family Finder test has received a haplogroup or your STR-derived haplogroup has been updated, you’ll receive an email notification with a link to a short, less than 2-minute video explaining what you’re receiving.

You can also expect emails in the following days with links to additional short videos. If you’d like to watch the videos now, click here.

You can also check your results, of course. If you should have received an email and didn’t, check your spam folder, and if it’s not there, notify FamilyTreeDNA in case your email has managed to get on the bounce list.

Group project administrators will receive notifications when a haplogroup is updated for a member in a project that they manage. This doesn’t just apply to Family Finder haplogroup updates for STR results – notifications will arrive when Big Y haplogroups are updated, too.

Emails about haplogroup updates will include both the old and the updated haplogroup.

Haplogroups may change as other testers receive results, forming a new haplogroup. The Big Y-700 test is evergreen, meaning as the Y tree grows, testers’ results are updated on an ongoing basis.

New View

Let’s take a look at what customers will receive.

In one of my surname projects, one male has taken a Family Finder test, but not the Y DNA test.

Several other men in that same paternal line, who are clearly related (including his brother), have taken Y DNA tests – both STR and the Big Y-700.

We have men who have taken the Big Y-700 test, STR tests only (no Big Y), and one with only a Family Finder test, so let’s compare all three, beginning with the man who has taken a Family Finder test but no Y DNA tests.

He has now been assigned to haplogroup I-BY1031, thanks to his Family Finder test.

Before today, because he has not taken a Y DNA test, he had no haplogroup or Y DNA Results section on his personal page.

Today, he does. Of course, he doesn’t have STR results or matches, but he DOES have confirmed SNP results, at least part way down the tree.

He can view these results on the Haplotree & SNPs tab or in Discover. Let’s look at both.

Haplotree & SNPs

By clicking on the Haplotree & SNPs link, you can view the results by variants (mutations,) as shown below, or by countries, surnames, or recommended projects for each haplogroup.

Of course, as more Family Finder results are rolled out, the more names and countries will appear on the Haplotree.

Recommended Projects

It’s easy to determine which haplogroup projects would be a good fit for people with these new haplogroups to join.

Just view by Recommended Projects, then scan up that column above the selected haplogroup. You can even just click right there to join. It’s that easy!

Results still won’t show on the public project page, because these testers don’t have STR results to display. Perhaps this will encourage additional testing in order to match with other men.

Download SNP Results

If you’re interested, you can download your SNP results in spreadsheet format.

I’m only showing four of my cousin’s positive SNPs, but FamilyTreeDNA was able to extract 358 positive SNPs to assign him to haplogroup I-BY1031.

Are Family Finder Haplogroups Better Than STR Test Predicted Haplogroups?

How do Family Finder haplogroups stack up against STR-predicted haplogroups?

Viewing the Y DNA results of related cousins who have taken STR tests, but not the Big Y-700, we see that their Y DNA haplogroup was predicted as I-M253.

We also know that the haplogroup determined by the Big Y-700 for this line is I-BY73911.

How can we use this information beneficially, and what does it mean?

Discover

Family Finder haplogroups can access Discover, which I wrote about, here.

Clicking on the Discover link takes you to your haplogroup story.

Let’s look at the new Family Finder Haplogroup Story for this tester.

Haplogroup I-BY1031 is about 3100 years old and is found in England, Sweden, the US, and 14 other countries. Of course, as more Family Finder haplogroups are provided to customers, this information will change for many haplogroups, so check back often.

Of course, you’ll want to review every single tab, which are chapters in your ancestral story! The Time Tree shows your haplogroup age in perspective to other haplogroups and their formation, and Ancient Connections anchors haplogroups through archaeology.

You can share any Discover page in several ways. This is a good opportunity to excite other family members about the discoveries revealed through DNA testing and genealogy. Prices are reduced right now with the Holiday Sale, too, so it’s a great time to gift someone else or yourself.

Compare – How Good is Good?

Ok, so how much better is the Family Finder haplogroup than the STR-predicted haplogroup, and how much better is the Big Y-700 haplogroup than the other two?

I’ll use the Discover “Compare” feature to answer these questions.

First, let’s compare the STR-predicted haplogroup of I-M253 to the Family Finder haplogroup of I-BY1031.

I clicked on Compare and entered the haplogroup I wish to compare to I-BY1031.

I-M253 I-BY1031 I-BY73199
Haplogroup Source STR Predicted Family Finder Big Y-700
Formation Year 2600 BCE 1100 BCE 1750 CE
Age – Years ago 4600 3100 270
Era Stone Age, Metal Age Metal Age Modern
Ancestral Locations England, Sweden, Germany, UK, +100 Sweden, England, US, +14 Netherlands
Tested Descendants 26,572 121 2
Branches 6779 69 0 – this is the pot-of-gold end leaf on the branch today

I created this chart to compare the major features of all three haplogroups.

The STR-predicted haplogroup, I-M253, takes you to about 2,600 BCE, or about 4,600 years ago. The Family Finder haplogroup shifts that significantly to about 1100 BCE, or 3100 years ago, so it’s about 1500 years more recent. However, the Big Y haplogroup takes you home – from 3100 years ago to about 270 years ago.

Notice that there’s a LOT of room for refinement under haplogroup I-M253. A Big Y tester might wind up on any of those 6779 branches, and might well be assigned to a newly formed branch with his test. The Family Finder haplogroup, I-BY1031, which was, by the way, discovered through Big Y testing, moved the autosomal test taker forward 1500 years where there are 121 descendants in 69 branches. The Big Y-700 test is the most refined possible, moving you directly into a genealogically relevant timeframe with a very specific location.

I-M253 is found in over 100 countries, I-BY1031 in 17 and I-BY73199 is found only in one – the Netherlands.

Based on confirmed genealogy, the common ancestor of the two men who have Big Y-700 haplogroup I-BY73199 was a man named Hendrik Jans Ferwerda, born in 1806 in the Netherlands, so 217 years ago. Of course, that haplogroup itself could have been born a generation or two before Hendrik. We simply won’t know for sure until more men test. More testers refine the haplotree, haplogroup ages, and refine our genealogy as well.

Haplogroup Comparison and Analysis

Let’s look at the Discover “Compare” feature of the three haplogroups from my family line from the Netherlands. Please note that your results will differ because every haplogroup is different, but this is a good way to compare the three types of haplogroup results and an excellent avenue to illustrate why testing and upgrades are so important.

The haplogroup ages are according to the Discover Time Tree.

Y-Adam to Haplogroup I1 I-M253 STR Haplogroup  to I-BY1031 Family Finder Haplogroup I-BY1031 Family Finder Haplogroup to BY73199 Big Y Haplogroup
Y-Adam (haplogroup A-PR2921) lived about 234,000 years ago
A0-T
A1
A1b
CT
CF
F
GHIJK
HIJK
IJK
IJ
I
M170
Z2699
L840
I1 I1
I-M253 lived about 4600 years ago
DF29
Z58
Z2041
Z2040
Z382
Y3643
Y2170
FT92441
FT45372
PH1178
BY1031 I-BY1031 lived about 3100 years ago
FT230048
BY65928
BY61100
I-BY73199 lived about 270 years ago

 All of the base haplogroups in the first column leading to Haplogroup I span the longest elapsed time, about 230,000 years, from Y-Adam to I-M253, the STR-predicted haplogroup, but are the least relevant to contemporary genealogy. They do tell us where we came from more distantly.

The second column moves you about 1500 years forward in time to the Family Finder confirmed haplogroup, reducing the location from pretty much everyplace in Europe (plus a few more locations) with more than 6700 branches, to 69 branches in only 17 countries.

With the fewest haplogroups, the third column spans the most recent 2800 years, bringing you unquestionably into the genealogically relevant timeframe, 270 years ago, in only one country where surnames apply.

If we had more testers from the Netherlands or nearby regions, there would probably be more branches on the tree between BY1031 and BY73199, the Big Y-700 haplogroup.

The second column is clearly an improvement over the first column which gets us to I-M253. The Family Finder upgrade from I-M253 to BY1031 provides information about our ancestors 3000-4500 years ago, where they lived and culturally, what they were doing. Ancient Connections enhance that understanding.

But the third column moves into the modern area where surnames are relevant and is the holy grail of genealogy. It’s a journey to get from Adam to the Netherlands in one family 270 years ago, but we can do it successfully between Family Finder and the Big Y-700.

Family Finder Matching

Given that these new haplogroups result from Family Finder, how do these results show in Family Finder matching? How do we know if someone with a haplogroup has taken a Y DNA test or if their haplogroup is from their Family Finder test?

  • All Family Finder haplogroups will show in the results for people who tested at FamilyTreeDNA as soon as they are all rolled out
  • All MyHeritage and Vitagene uploads, because they are processed by the Gene by Gene lab, will be shown IF they have purchased the unlock.
  • No Ancestry or 23andMe haplogroups will be shown to Family Finder matches

To determine whether or not your matches’ haplogroups result from a Y DNA test or a Family Finder haplogroup, on your Family Finder match page, look just beneath the name of your matches.

The first man above received the Family Finder haplogroup. You can see he has no other tests listed. The second man has taken the Big Y-700 test. You can see that he has a different haplogroup, and if you look beneath his name, you’ll see that he took the Big Y-700 test.

For other men, you may see the 67 or 111 marker tests, for example, so you’ll know that they are available for Y-DNA matching. That may be important information because you can then visit the appropriate surname project to see if they happen to be listed with an earliest known ancestor.

After the rollout is complete, If you have a male Family Finder match with no haplogroup shown, you know that:

  • They did not test at FamilyTree DNA
  • If they uploaded from MyHeritage or Vitagene, they did not unlock the advanced Family Finder features
  • Or, they tested at either 23andMe or Ancestry, and uploaded their results

You can always reach out to your match and ask.

How to Use This Information

There are several great ways to utilize this new information.

I have a roadblock with my Moore line. Moore is a common surname with many, many origins, so I have autosomal matches to several Moore individuals who may or may not be from my Moore line.

I do know the base haplogroup of my Moore men, but I do not have a Big Y, unfortunately, and can’t upgrade because the tester is deceased. (I wish I had ordered the Big Y out the gate, but too late now.)

As soon as the results are complete for all of the testers, I’ll be able, by process of elimination to some extent, focus ONLY on the testers who fall into Family Finder haplogroup of my Moore cousins, or at least haplogroup close for Ancestry or 23andMe upload customers. In other words, I can eliminate the rest.

I can then ask the men with a similar haplogroup to my proven Moore cousins for more information, including whether they would be willing to take a Y DNA test.

  • Second, as soon as the Family Finder processing is complete, I will know that all male Family Finder matches and uploads from MyHeritage and Vitagene that have paid for the unlock will have haplogroups displayed on the Family Finder Match page. Therefore, if there’s a male Moore with no haplogroup, I can reach out to see where they tested and if a haplogroup has been assigned, even if it’s from Ancestry or 23andMe and isn’t displayed in Family Finder.

If so, and they share the haplogroup with me, I’ll be able to include or exclude them. If included, I can then ask if they would consider taking a Y DNA test.

  • Third, for lines I don’t yet have Y DNA testers for, I can now peruse my matches, and my cousins’ matches for that line. See items one and two, above. Even if they don’t reply or agree to Y DNA testing, at least now I have SOME haplogroup for that missing line.

Discover will help me flesh out the information I have, narrow regions, find projects, look at ancient DNA for hints, and more.

  • Fourth, the haplogroups themselves. I don’t know how many million tests FamilyTreeDNA has in their database, but if we assume that half of those are male, some percentage won’t have taken a Y DNA test at all. We’ll be able to obtain Y-DNA information for lines where there may be no other living descendant. I have at least one like that. He was the end of the surname line and is deceased, with no sons.

I’m literally ecstatic that I’ll be able to obtain at least something for that line. If it’s anything like my example Netherlands lineage, the Family Finder haplogroup may be able to point me to an important region of Europe – or maybe someplace else very unexpected.

The Bottom Line

Here’s the bottom line. You don’t know what you don’t know – and our ancestors are FULL of surprises.

I can’t even begin to tell you how MUCH I’m looking forward to this haplogroup rollout.

To prepare, I’m making a list of my genealogical lines:

  • If the paternal line, meaning surname line, is represented by any match in any database
  • If that line is represented by a known person in the FamilyTreeDNA database and by whom
  • If they or someone from that line has joined a surname or other FamilyTreeDNA project, and if so, which one
  • If they’ve taken a Y DNA test, and what kind – watch STR results for an updated haplogroup
  • If they’ve taken a Family Finder test – my cousin is a good example of a known individual whose kit I can watch for a new haplogroup
  • Old and new haplogroup, if applicable

If my only relative from that line is in another vendor’s database, I’ll ask if they will upload their file to FamilyTreeDNA – and explain why by sharing this article. (Feel free to do the same.) A Y DNA haplogroup is a good incentive, and I would be glad to pay for the unlock at FamilyTreeDNA for cousins who represent Y and mitochondrial DNA lines I don’t already have.

One way I sweeten the pie is to offer testing scholarships to select lines where I need either the Y DNA or mitochondrial DNA of relevant ancestors. It’s a good thing these haplogroups are being rolled out a few thousand at a time! I need to budget for all the scholarships I’ll want to offer.

I feel like I won the lottery, and FamilyTreeDNA is giving me a free haplogroup encyclopedia of information about my ancestors through my cousins – even those who haven’t taken Y DNA tests. I can’t even express how happy this makes me.

What lines do you want to discover more about, and what is your plan? Tests are on sale now if you need them!

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Which DNA Test Should I Buy? And Why?

Which DNA test should I buy, and why?

I receive questions like this often. As a reminder, I don’t take private clients anymore, which means I don’t provide this type of individual consulting or advice. However, I’m doing the next best thing! In this article, I’m sharing the step-by-step process that I utilize to evaluate these questions so you can use the process too.

It’s important to know what questions to ask and how to evaluate each situation to arrive at the best answer for each person.

Here’s the question I received from someone I’ll call John. I’ve modified the wording slightly and changed the names for privacy.

I’m a male, and my mother was born in Charleston, SC. My maternal grandmother’s maiden name was Jones and a paternal surname was Davis. The family was supposed to have been Black, Dutch, Pennsylvania Dutch, and Scots-Irish…only once was I told I was 3/16 Indian, with Davis being 3/4 and Jones being full Indian.

Do I have enough reasonable information to buy a test, and which one?

Please note that it’s common for questions to arrive without all the information you need to provide a sound answer – so it’s up to you to ask those questions and obtain clarification.

Multiple Questions

There are actually multiple questions here, so let me parse this a bit.

  1. John never mentioned what his testing goal was.
  2. He also never exactly said how the paternal line of Davis was connected, so I’ve made an assumption. For educational purposes, it doesn’t matter because we’re going to walk through the evaluation process, which is the same regardless.
  3. John did not include a tree or a link to a tree, so I created a rudimentary tree to sort through this. I need the visuals and normally just sketch it out on paper quickly.
  4. Does John have enough information to purchase a test?
  5. If so, which test?

There is no “one size fits all” answer, so let’s discuss these one by one.

Easy Answers First

The answer to #4 is easy.

Anyone with any amount of information can purchase a DNA test. Adoptees do it all the time, and they have no prior information.

So, yes, John can purchase a test.

The more difficult question is which test, because that answer depends on John’s goals and whether he’s just looking for some quick information or really wants to delve into genealogy and learn. Neither approach is wrong.

Many people think they want a quick answer –  and then quickly figure out that they really want to know much more about their ancestors.

I wrote an article titled DNA Results – First Glances at Ethnicity and Matching for new testers, here.

Goals

Based on what John said, I’m going to presume his goals are probably:

  • To prove or disprove the family oral history of Black, Dutch, Pennsylvania Dutch (which is actually German,) Scots-Irish, and potentially Native American.
  • John didn’t mention actual genealogy, which would include DNA matches and trees, so we will count that as something John is interested in secondarily. However, he may need genealogy records to reach his primary goal.

If you’re thinking, “The process of answering this seemingly easy question is more complex than I thought,” you’d be right.

Ethnicity in General

It sounds like John is interested in ethnicity testing. Lots of people think that “the answer” will be found there – and sometimes they are right. Often not so much. It depends.

The great news is that John really doesn’t need any information at all to take an autosomal DNA test, and it doesn’t matter if the test-taker is male or female.

To calculate each tester’s ethnicity, every testing company compiles their own reference populations, and John will receive different results at each of the major companies. Each company updates their ethnicity results from time to time as well, and they will change.

Additionally, each company provides different tools for their customers.

The ethnicity results at different companies generally won’t match each other exactly, and sometimes the populations look quite different.

Normally, DNA from a specific ancestor can be found for at least 5 or 6 generations. Of course, that means their DNA, along with the DNA from all of your other ancestors is essentially combined in a communal genetic “pot” of your chromosomes, and the DNA testing company needs to sort it out and analyze your DNA for ethnicity.

DNA descended from ancestors, and their populations, further back in people’s trees may not be discerned at all using autosomal DNA tests.

A much more specific “ethnicity” can be obtained for both the Y-DNA line, which is a direct patrilineal line for men (blue arrow,) and the mitochondrial DNA line (pink arrows,) which is a direct matrilineal line for everyone, using those specific tests.

We will discuss both of those tests after we talk about the autosomal tests available from the four major genealogy DNA testing companies. All of these tools can and should be used together.

Let’s Start with Native American

Let’s evaluate the information that John provided.

John was told that he “was 3/16 Indian, with Davis being 3/4 and Jones being full Indian.”

We need to evaluate this part of his question slightly differently.

I discussed this in the article, Ancestral DNA Percentages – How Much of Them is in You?

First, we need to convert generations to 16ths.

You have two ancestors in your parent’s generation, four in your grandparents, and so forth. You have 16 great-great-grandparents. So, if John was 3/16th Native, then three of his great-great-grandparents would have been fully Native, or an equivalent percentage. In other words, six ancestors in that generation could have been half-Native. Based on what John said, they would have come from his mother’s side of the tree. John is fortunate to have that much information to work with.

He told us enough about his tree that we can evaluate the statement that he might be 3/16ths Native.

Here’s the tree I quickly assembled in a spreadsheet based on John’s information.

His father, at left, is not part of the equation based on the information John provided.

On his mother’s side, John said that Grandfather Davis is supposed to be three-quarters Native, which translates to 12/16ths. Please note that it would be extremely beneficial to find a Y-DNA tester from his Davis line, like one of his mother’s brothers, for example.

John said that his Grandmother Jones is supposed to be 100% Native, so 16/16ths.

Added together, those sum to 28/32, which reduces down to 14/16th or 7/8th for John’s mother.

John would have received half of his autosomal DNA from his mother and half from his non-Native father. That means that if John’s father is 100% non-Native, John would be half of 14/16ths or 7/16ths, so just shy of half Native.

Of course, we know that we don’t always receive exactly 50% of each of our ancestors’ DNA (except for our parents,) but we would expect to see something in the ballpark of 40-45% Native for John if his grandmother was 100% Native and his grandfather was 75%.

Using simple logic here, for John’s grandmother to be 100% Native, she would almost assuredly have been a registered tribal member, and the same if his grandfather was 75% Native. I would think that information would be readily available and well-known to the family – so I doubt that this percentage is accurate. It would be easy to check, though, on various census records during their lifetimes where they would likely have been recorded as “Indian.” They might have been in the special “Indian Census” taken and might be living on a reservation.

It should also be relatively easy to find their parents since all family members were listed every ten years in the US beginning with the 1850 census.

The simple answer is that if John’s grandparents had as much Native as reported, he would be more than 3/16th – so both of these factoids cannot simultaneously be accurate. But that does NOT mean neither is accurate.

John could be 7/8th or 40ish%, 3/16th or 18ish%, or some other percentage. Sometimes, where there is smoke, there is fire. And that seems to be the quandary John is seeking to resolve.

Would  Ethnicity/Population Tests Show This Much Native?

Any of the four major testing companies would show Native for someone whose percentage would be in the 40% or 18% ballpark.

The easiest ethnicities to tell apart from one another are continental-level populations. John also stated that he thinks he may also have Black ancestry, plus Dutch, Pennsylvania Dutch (German), and Scots-Irish. It’s certainly possible to verify that using genealogy, but what can DNA testing alone tell us?

How far back can we expect to find ethnicities descending from particular ancestors?

In this table, you can see at each generation how many ancestors you have in that generation, plus the percentage of DNA, on average, you would inherit from each ancestor.

All of the major DNA testing companies can potentially pick up small trace percentages, but they don’t always. Sometimes one company does, and another doesn’t. So, if John has one sixth-generation Native American ancestor, he would carry about 1.56% Native DNA, if any.

  • Sometimes a specific ethnicity is not found because, thanks to random recombination, you didn’t inherit any of that DNA from those ancestors. This is why testing your parents, grandparents, aunts, uncles, and siblings can be very important. They share your same ancestors and may have inherited DNA that you didn’t that’s very relevant to your search.
  • Sometimes it’s not found because the reference populations and algorithms at that testing company aren’t able to detect that population or identify it accurately, especially at trace levels. Every DNA testing company establishes their own reference populations and writes internal, proprietary ethnicity analysis algorithms.
  • Sometimes it’s not found because your ancestor wasn’t Native or from that specific population.
  • Sometimes it’s there, but your population is called something you don’t expect.

For example, you may find Scandinavian when your ancestor was from England or Ireland. The Vikings raided the British Isles, so while some small amount of Scandinavian is not what you expect, that doesn’t mean it‘s wrong. However, if all of your family is from England, it’s not reasonable to have entirely Scandinavian ethnicity results.

It’s also less likely as each generation passes by that the information about their origins gets handed down accurately to following generations. Most non-genealogists don’t know the names of their great-grandparents, let alone where their ancestors were from.

Using a 25-year average generation length, by the 4th generation, shown in the chart above, you have 16 ancestors who lived approximately 100 years before your parents were born, so someplace in the mid-1800s. It’s unlikely for oral history from that time to survive intact. It’s even less likely from a century years earlier, where in the 7th generation, you have 128 total ancestors.

The best way to validate the accuracy of your ethnicity estimates is by researching your genealogy. Of course, you need to take an ethnicity test, or two, in order to have results to validate.

Ethnicity has a lot more to offer than just percentages.

Best Autosomal Tests for Native Ethnicity

Based on my experience with people who have confirmed Native ancestry, the two best tests to detect Native American ethnicity, especially in smaller percentages, are both FamilyTreeDNA and 23andMe.

Click images to enlarge

In addition to percentages, both 23andMe and FamilyTreeDNA provide chromosome painting for ethnicity, along with segment information in download files. In other words, they literally paint your ethnicity results on your chromosomes.

They then provide you with a file with the “addresses” of those ethnicities on your chromosomes, which means you can figure out which ancestors contributed those ethnicity segments.

The person in the example above, a tester at FamilyTreeDNA, is highly admixed with ancestors from European regions, African regions and Native people from South America.

Trace amounts of Native American with a majority of European heritage would appear more like this.

You can use this information to paint your chromosome segments at DNAPainter, along with your matching segments to other testers where you can identify your common ancestors. This is why providing trees is critically important – DNA plus ancestor identification with our matches is how we confirm our ancestry.

This combination allows you to identify which Native (or another ethnicity) segments descended from which ancestors. I was able to determine which ancestor provided that pink Native American segment on chromosome 1 on my mother’s side.

I’ve provided instructions for painting ethnicity segments to identify their origins in specific ancestors, here.

Autosomal and Genealogy

You may have noticed that we’ve now drifted into the genealogy realm of autosomal DNA testing. Ethnicity is nice, but if you want to know who those segments came from, you’ll need:

  • Autosomal test matching to other people
  • To identify your common ancestor with as many matches as you can
  • To match at a company who provides you with segment information for each match
  • To work with DNAPainter, which is very easy

The great news is that you can do all of that using the autosomal tests you took for ethnicity, except at Ancestry who does not provide segment information.

Best Autosomal Test for Matching Other Testers

The best autosomal test for matching may be different for everyone. Let’s look at some of the differentiators and considerations.

If you’re basing a testing recommendation solely on database size, which will probably correlate to more matches, then the DNA testing vendors fall into this order:

If you’re basing that recommendation on the BEST, generally meaning the closest matches for you, there’s no way of knowing ahead of time. At each of the four DNA testing companies, I have very good matches who have not tested elsewhere. If I weren’t in all four databases, I would have missed many valuable matches.

If you’re basing that recommendation on which vendor began testing earliest, meaning they have many tests from people who are now deceased, so you won’t find their autosomal tests in other databases that don’t accept uploads, the recommended testing company order would be:

If you’re basing that recommendation on matches to people who live in other countries, the order would be:

Ancestry and 23andMe are very distant third/fourth because they did not sell widely outside the US initially and still don’t sell in as many countries as the others, meaning their testers’ geography is more limited. However, Ancestry is also prevalent in the UK.

If you’re basing that recommendation on segment information and advanced tools that allow you to triangulate and confirm your genetic link to specific ancestors, the order would be:

Ancestry does NOT provide any segment information.

If you’re basing that recommendation on unique tools provided by each vendor, every vendor has something very beneficial that the others don’t.

In other words, there’s really no clear-cut answer for which single autosomal DNA test to order. The real answer is to be sure you’re fishing in all the ponds. The fish are not the same. Unique people test at each of those companies daily who will never be found in the other databases.

Test at or upload your DNA to all four DNA testing companies, plus GEDmatch. Step-by-step instructions for downloading your raw data file and uploading it to the DNA testing companies who accept uploads can be found, here.

Test or Upload

Not all testing companies accept uploads of raw autosomal DNA data files from other companies. The good news is that some do, and it’s free to upload and receive matches.

Two major DNA testing companies DO NOT accept uploads from other companies. In other words, you have to test at that company:

Two testing companies DO accept uploads from the other three companies. Uploads and matching are free, and advanced features can be unlocked very cost effectively.

  • FamilyTreeDNA – free matching and $19 unlock for advanced features
  • MyHeritage – free matching and $29 unlock.for advanced features

I recommend testing at both 23andMe and Ancestry and uploading one of those files to both FamilyTreeDNA and MyHeritage, then purchasing the respective unlocks.

GEDmatch

GEDmatch is a third-party matching site, not a DNA testing company. Consider uploading to GEDmatch because you may find matches from Ancestry who have uploaded to GEDmatch, giving you access to matching segment information.

Other Types of DNA

John provided additional information that may prove to be VERY useful. Both Y-DNA and mitochondrial DNA can be tested as well and may prove to be more useful than autosomal to positively identify the origins of those two specific lines.

Let’s assume that John takes an autosomal test and discovers that indeed, the 3/16th Native estimate was close. 3/16th equates to about 18% Native which would mean that three of his 16 great-great-grandparents were Native.

John told us that his Grandmother Jones was supposed to be 100% Native.

At the great-great-grandparent level, John has 16 ancestors, so eight on his mother’s side, four from maternal grandmother Jones and four from his maternal grandfather Davis.

John carries the mitochondrial DNA of his mother (red boxes and arrows,) and her mother, through a direct line of females back in time. John also carries the Y-DNA of his father (dark blue box, at left above, and blue arrows below.)

Unlike autosomal DNA which is admixed in every generation, mitochondrial DNA (red arrows) is inherited from that direct matrilineal line ONLY and never combines with the DNA of the father. Mothers give their mitochondrial DNA to both sexes of their children, but men never contribute their mitochondrial DNA to offspring. Everyone has their mother’s mitochondrial DNA.

Because it never recombines with DNA from the father, so is never “watered down,” we can “see” much further back in time, even though we can’t yet identify those ancestors.

However, more importantly, in this situation, John can test his own mitochondrial DNA that he inherited from his mother, who inherited it from her mother, to view her direct matrilineal line.

John’s mitochondrial DNA haplogroup that will be assigned during testing tells us unquestionably whether or not his direct matrilineal ancestor was Native on her mother’s line, or not. If not, it may well tell us where that specific line originated.

You can view the countries around the world where Y-DNA haplogroups are found, here, and mitochondrial haplogroups, here.

If John’s mitochondrial DNA haplogroup is Native, that confirms that one specific line is Native. If he can find other testers in his various lines to test either their Y-DNA or mitochondrial DNA, John can determine if other ancestors were Native too. If not, those tests will reveal the origins of that line, separate from the rest of his genealogical lines.

Although John didn’t mention his father’s line, if he takes a Y-DNA test, especially at the Big Y-700 level, that will also reveal the origins of his direct paternal line. Y-DNA doesn’t combine with the other parent’s DNA either, so it reaches far back in time too.

Y-DNA and mitochondrial DNA tests are laser-focused on one line each, and only one line. You don’t have to try to sort it out of the ethnicity “pot,” wondering which ancestor was or was not Native.

My Recommendation

When putting together a testing strategy, I recommend taking advantage of free uploads and inexpensive unlocks when possible.

  • To confirm Native American ancestry via ethnicity testing, I recommend testing at 23andMe and uploading to FamilyTreeDNA, then purchasing the $19 unlock. The free upload and $19 unlock are less expensive than testing there directly.
  • For matching, I recommend testing at Ancestry and uploading to MyHeritage, then unlocking the MyHeritage advanced features for $29, which is less expensive than retesting. Ancestry does not provide segment information, but MyHeritage (and the others) do.

At this point, John will have taken two DNA tests, but is now in all four databases, plus GEDmatch if he uploads there.

  • For genealogy research on John’s lines to determine whether or not his mother’s lines were Native, I recommend an Ancestry and a MyHeritage records subscription, plus using WikiTree, which is free.
  • To determine if John’s mother’s direct matrilineal female line was Native, I recommend that John order the mitochondrial DNA test at FamilyTreeDNA.
  • When ordering multiple tests, or uploading at FamilyTreeDNA, be sure to upload/order all of one person’s tests on the same DNA kit so that those results can be used in combination with each other.

Both males and females can take autosomal and mitochondrial DNA tests.

  • To discover what he doesn’t know about his direct paternal, meaning John’s surname line – I recommend the Big Y-700 test at FamilyTreeDNA.

Only males can take a Y-DNA test, so women would need to ask their father, brother, or paternal uncle, for example, to test their direct paternal line.

  • If John can find a male Davis from his mother’s line, I recommend that he purchase the Big Y-700 test at FamilyTreeDNA for that person, or check to see if someone from his Davis line may have already tested by viewing the Davis DNA Project. Like with mitochondrial DNA, the Y-DNA haplogroup will tell John the origins of his direct Davis male ancestor – plus matching of course. He will be able to determine if they were Native, and if not, discover the origins of the Davis line.
  • For assigning segments to ancestors and triangulating to confirm descent from a common ancestor, I recommend 23andMe, MyHeritage, FamilyTreeDNA and GEDmatch, paired with DNAPainter as a tool.

Shopping and Research List

Here are the tests and links recommended above:

More Than He Asked

I realize this answer is way more than John expected or even knew to ask. That’s because there is often no “one” or “one best” answer. There are many ways to approach the question after the goal is defined, and the first “answer” received may be a bit out of context.

For example, let’s say John has 2% Native ancestry and took a test at a vendor who didn’t detect it. John would believe he had none. But a different vendor might find that 2%. If it’s on his mother’s direct matrilineal line, mitochondrial DNA testing will confirm, or refute Native, beyond any doubt, regardless of autosomal ethnicity results – but only for that specific ancestral line.

Autosomal DNA can suggest Native across all your DNA, but Y-DNA and mitochondrial DNA confirm it for each individual ancestor.

Even when autosomal testing does NOT show Native American, or African, for example, it’s certainly possible that it’s just too far back in time or has not been passed down during random recombination, but either Y-DNA or mitochondrial DNA will unquestionably confirm (or refute) the ancestry in question if the right person is tested.

This is exactly why I attempt to find a cousin who descends appropriately from every ancestor and provide testing scholarships. It’s important to obtain Y-DNA and mitochondrial DNA information for each ancestor.

Which Test Should I Order?

What steps will help you decide which test or tests to take?

  1. Define your testing goal.
  2. Determine if your Y-DNA or mitochondrial DNA will help answer the question.
  3. Determine if you need to find ancestors another generation or two back in time to get the most benefit from DNA testing. In our example, if John discovered that both of his grandparents were enrolled tribal members, that’s huge, and the tribe might have additional information about his family.
  4. Subscribe to Ancestry and MyHeritage records collections as appropriate to perform genealogical research. Additional information not only provides context for your family, it also provides you with the ability to confirm or better understand your ethnicity results.
  5. Extend your tree so that you can obtain the best results from the three vendors who support trees; Ancestry, FamilyTreeDNA, and MyHeritage. All three use trees combined with DNA tests to provide you with additional information.
  6. Order 23andMe and Ancestry autosomal DNA tests.
  7. Either test at or upload one of those tests to MyHeritage, FamilyTreeDNA, and GEDmatch.
  8. If a male, order the Big Y-700 DNA test. Or, find a male from your ancestral line who has taken or will take that test. I always offer a testing scholarship and, of course, share the exciting results!
  9. Order a mitochondrial DNA test for yourself and for appropriately descended family members to represent other ancestors. Remember that your father (and his siblings) all carry your paternal grandmother’s mitochondrial DNA. That’s often a good place to start after testing your own DNA.
  10. If your parents or grandparents are alive, or aunts and uncles, test their autosomal DNA too. They are (at least) one generation closer to your ancestors than you are and will carry more of your ancestors’ DNA.
  11. Your siblings will carry some of your ancestors’ DNA that you do not, so test them too if both of your parents aren’t available for testing.

Enjoy!!!

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