Category Archives: FamilyTreeDNA

In Search of…Vendor Features, Strengths, and Testing Strategies

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This is the third in our series of articles about searching for unknown close family members, specifically; parents, grandparents, or siblings. However, these same techniques can be applied to ancestors further back in time too.

In this article, we are going to discuss your goals and why testing or uploading to multiple vendors is advantageous – even if you could potentially solve the initial mystery at one vendor. Of course, the vendor you test with first might not be the vendor where the mystery will be solved, and data from multiple vendors might just be the combination you need.

Testing Strategy – You Might Get Lucky

I recommended in the first article that you go ahead and test at the different vendors.

Some people asked why, and specifically, why you wouldn’t just test at one vendor with the largest database first, then proceed to the others if you needed to.

That’s a great question, and I want to discuss the pros and cons in this article more specifically.

Clearly, that is one strategy, but the approach you select might differ based on a variety of considerations:

  • You may only be interested in obtaining the name of the person you are seeking – or – you may be interested in finding out as much as possible.
  • You may find that your best match at one company is decidedly unhelpful, and may even block you or your efforts, while someone elsewhere may be exactly the opposite.
  • Solving your mystery may be difficult and painful at one vendor, but the answer may be infinitely easier at a different vendor where the answer may literally be waiting.
  • There may not be enough, or the right information, or matches, at any one vendor, but the puzzle may be solvable by combining information from multiple vendors and tests. Every little bit helps.
  • You may have a sense of urgency, especially if you hope to meet the person and you’re searching for parents, siblings or grandparents who may be aging.
  • You may be cost-sensitive and cannot afford more than one test at a time. Fortunately, our upload strategy helps with that too. Also, watch for vendor sales or bundles.

From the time you order your DNA test, it will be about 6-8 weeks, give or take a week or two in either direction, before you receive results.

When those results arrive, you might get lucky, and the answer you seek is immediately evident with no additional work and just waiting for you at the first testing company.

If that’s the case, you got lucky and hit the jackpot. If you’re searching for both parents, that means you still have one parent to go.

Unidentified grandparents can be a little more difficult, because there are four of them to sort between.

If you discover a sibling or half-sibling, you still need to figure out who your common parent is. Sometimes X, Y, and mitochondrial DNA provides an immediate answer and is invaluable in these situations.

It’s more likely that you’ll find a group of somewhat more distant relatives. You may be able to figure out who your common grandparents or great-grandparents are, but not your parent(s) initially. Often, the closer generation or two is actually the most difficult because you’re dealing with contemporary records which are not publicly available, fewer descendants, and the topic may be very uncomfortable for some people. It’s also complicated because you’re often not dealing with “full” relationships, but “half,” as in half-sibling, half-niece, half-1C, etc.

You may spend a substantial amount of time trying to solve this puzzle at the first vendor before ordering your next test.

That second test will also take about 6-8 weeks, give or take. I recommend that you order the first two autosomal tests, now.

Order Your First Two Autosomal Tests

The two testing companies with the largest autosomal databases for comparison, Ancestry, and 23andMe, DO NOT accept DNA file uploads from other companies, so you’ll need to test with each individually.

Fortunately, you CAN transfer your autosomal DNA tests to both MyHeritage and FamilyTreeDNA, for free.

You will have different matches at each company. Some people will be far more responsive and helpful than others.

I recommend that you go ahead and order both the Ancestry and 23andMe tests initially, then upload the first one that comes back with results to both FamilyTreeDNA and MyHeritage. Complete, step-by-step download/upload instructions can be found here.

You can also upload your DNA file to a fifth company, Living DNA, but they are significantly smaller and heavily focused on England and Great Britain. However, if that’s where you’re searching, this might be where you find important matches.

You can also upload to GEDMatch, a popular third-party database, but since you’re going to be in the databases of the four major testing companies, there is little to be gained at GEDMatch in terms of people who have not tested at one of the major companies. Do NOT upload to GEDMatch INSTEAD of testing or uploading to the four major sites, as GEDMatch only has a small fraction of the testers in each of the vendor databases.

What GEDMatch does offer is a chromosome browser – something that Ancestry does NOT offer, along with other clustering tools which you may find useful. I recommend GEDMatch in addition to the others, if needed or desired.

Ordering Y and Mitochondrial DNA Tests

We reviewed the basics of the different kinds of DNA, here.

Some people have asked why, if autosomal DNA shows relatives on all of your lines, would one would want to order specific tests that focus on just one line?

It just so happens that the two lines that Y and mitochondrial DNA test ARE the two lines you’re seeking – direct maternal – your mother (and her mother), and direct paternal, your father (and his father.)

These two tests are different kinds of DNA tests, testing a different type of DNA, and provide very focused information, and matches, not available from autosomal DNA tests.

For men, Y DNA can reveal your father’s surname, which can be an invaluable clue in narrowing paternal candidates. Knowing that my brother’s Y DNA matched several men with the surname of Priest made me jump for joy when he matched a woman of that same last name at another vendor.

Here’s a quote from one of the members of a Y DNA project where I’m the volunteer administrator:

“Thank you for your help understanding and using all 4 kinds of my DNA results. By piecing the parts together, I identified my father. Specifically, without Y DNA testing, and the Big Y test, I would not have figured out my parental connection, and then that my paternal line had been assigned to the wrong family. STR testing gave me the correct surname, but the Big Y test showed me exactly where I fit, and disproved that other line. I’m now in touch with my father, and we both know who our relatives are – two things that would have never happened otherwise.”

If you fall into the category of, “I want to know everything I can now,” then order both Y and mitochondrial DNA tests initially, along with those two autosomal tests.

You will need to order Y (males only) and mitochondrial DNA tests separately from the autosomal Family Finder test, although you should order on the same account as your Family Finder test at FamilyTreeDNA.

If you take the Family Finder autosomal test at FamilyTreeDNA or upload your autosomal results from another vendor, you can simply select to add the Y and mitochondrial DNA tests to your account, and they will send you a swab kit.

Conversely, you can order either a Y or mitochondrial DNA test, and then add a Family Finder or upload a DNA file if you’ve already taken an autosomal DNA test to that account too. Note – these might not be current prices – check here for sales.

You will want all 3 of your tests on the same account so that you can use the Advanced Matches feature.

Using Advanced Matches, you’ll be able to view people who match you on combinations of multiple kinds of tests.

For example, if you’re a male, you can see if your Y DNA matches also match you on the Family Finder autosomal test, and if so, how closely?

Here’s an example.

In this case, I requested matches to men with 111 markers who also match the tester on the Family Finder test. I discovered both a father and a full sibling, plus a few more distant matches. There were ten total combined matches to work with, but I’ve only shown five for illustration purposes.

This information is worth its weight in gold.

Is the Big Y Test Worth It?

People ask if the Big Y test is really worth the extra money.

The answer is, “it depends.”

If all you’re looking for are matching surnames, then the answer is probably no. A 37 or 111 marker test will probably suffice. Eventually, you’ll probably want to do the Big Y, though.

If you’re looking for exact placement on the tree, with an estimated distance to other men who have taken that test, then the answer is, “absolutely.” I wish the Big Y test had been available back when I was hunting for my brother’s biological family.

The Big Y test provides a VERY specific haplogroup and places you very accurately in your location on the Y DNA tree, along with other men of your line, assuming they have tested. You may find the surname, as well as being placed within a generation or a few of current in that family line.

Additionally, the Discover page provides estimates of how far in the past you share a common ancestor with other people that share the same haplogroup. This can be a HUGE boon to a male trying to figure out his surname line and how closely in time he’s related to his matches.

Big Y NPE Examples

Y DNA SNP mutations tested with the Big Y test accrue a mutation about every generation, or so. Sometimes we see mutations in every generation.

Here’s an example from my Campbell line. Haplogroups are listed in the top three rows.

I created this spreadsheet, but FamilyTreeDNA provides a block tree for Big Y testers. I’ve added the genealogy of the testers, with the various Big Y testers at the bottom and common ancestors above, in bold.

We have two red NPE lines showing. The MacFarlane tester matches M. Campbell VERY closely, and two Clark males match W. Campbell and other Campbells quite closely. We utilized autosomal plus the Y results to determine where the unknown parentage events occurred. Today, if you’re a Clark or MacFarlane male, or a male by any other surname who was fathered by a Y chromosome Campbell male (by any surname), you’ll know exactly where you fit in this group of testers on your direct paternal line.

Y DNA is important because men often match other men with the same surname, which is a HUGE clue, especially in combination with autosomal DNA results. I say “often,” because it’s possible that no one in your line has tested, or that your father’s surname is not his biological surname either.

Y and mitochondrial DNA matches can be HUGELY beneficial pieces of information either by confirming a close autosomal relationship on that line, or eliminating the possibility.

Lineage-Specific Population Information

In addition to matching other people, both Y and mitochondrial DNA tests provide you with lineage-specific population or “ethnicity” information for this specific line which helps you focus your research.

For example, if you view the Y DNA Haplogroup Origins shown for this tester, you’ll discover that these matches are Jewish.

The tester might not be Jewish on any other genealogical line, but they definitely have Jewish ancestry on their Y DNA, paternal, line.

The same holds true for mitochondrial DNA as well. The main difference with mitochondrial DNA is that the surname changes with each generation, haplogroups today (pre-Million Mito) are less specific, and fewer people have been tested.

Y and Mitochondrial DNA Benefits

Knowing your Y and mitochondrial DNA haplogroups not only arm you with information about yourself, they provide you with matching tools and an avenue to include or exclude people as your direct line paternal or maternal ancestors.

Your Y and mitochondrial DNA can also provide CRITICALLY IMPORTANT information about whether that direct line ancestor belonged to an endogamous population, and where they came from.

For example, both Jewish and Native populations are endogamous populations, meaning highly intermarried for many generations into the past.

Knowing that helps you adjust your autosomal relationship analysis.

Why Order Multiple Tests Initially Instead of Waiting?

If you’ve been adding elapsed time, two autosomal tests (Ancestry and 23andMe), two uploads (to FamilyTreeDNA and MyHeritage,) a Y DNA test, and a mitochondrial DNA test, if all purchased serially, one following the other, means you’ll be waiting approximately 6-8 months.

Do you want to wait 6-8 months for all of your results? Can you afford to?

Part of this answer has to do with what, exactly, you’re seeking, and how patient you are.

Only you can answer that question.

A Name or Information?

Are you seeking the name or identity of a person, or are you seeking information about that person?

Most people don’t just want to put a name to the person they are seeking – they want to learn about them and the rest of the family that door opens.

You will have different matches at each company. Even after you identify the person you seek, the people you match may have trees you can view, with family photos and other important information. (Remember, you can’t see living people in trees.) Your matches may have first-person information about your relative and may know them if they are living, or have known them.

Furthermore, you may have the opportunity to meet that person. Time delayed may not be able to be recovered or regained.

One cousin that I assisted discovered that his father had died just six weeks before he broke through that wall and made the connection.

Working with data from all vendors simultaneously will allow you to combine that data and utilize it together. Using your “best” matches at each company, augmented by X, Y, and/or mitochondrial DNA, can make MUCH shorter work of this search.

Your closest autosomal matches are the most important and insightful. In this series, I will be working with the top 15 autosomal results at each vendor, at least initially. This approach provides me with the best chance of meaningful close relationship discoveries.

Data and Vendor Results Integration

Here’s a table of my two closest maternal and paternal matches at the four major vendors. I can assign these to maternal or paternal sides, because I know the identity of my parents, and I know some of these people. If an adoptee was doing this, the top 4 could all be from one parent, which is why we work with the top 15 or so matches.

Vendor Closest Maternal Closest Paternal Comments
Ancestry 1C, 1C1R Half-1C, 2C I recognized both of the maternal and neither of the paternal.
23andMe 2C, 2C 1C1R, half-gr-niece Recognized both maternal, one paternal
MyHeritage Mother uploaded, 1C Half-niece, half-1C Recognized both maternal, one paternal
FamilyTreeDNA Mother tested, 1C1R Parent/child, half-gr-niece uploaded Recognized all 4

To be clear, I tested my mother’s mitochondrial DNA before she passed away, but because FamilyTreeDNA archives DNA samples for 25 years, as the owner/manager of her DNA kit, I was able to order the Family Finder test after she had passed away. Her tests are invaluable today.

Then, years later, I uploaded her results to MyHeritage.

If I was an adopted child searching for my mother, I would find her results in both databases today. She’ll never be at either 23andMe or Ancestry because she passed away before she could test there and they don’t accept uploads.

Looking at the other vendors, my half-niece at MyHeritage is my paternal half-sibling’s daughter. My half-sibling is deceased, so this is as close as I’ll ever get to matching her.

At 23andMe, the half-great-niece is my half-siblings grandchild.

It’s interesting that I have no matches to descendants of my other half-sibling, who is also deceased. Maybe I should ask if any of his children or grandchildren have tested. Hmmmm…..

You can see that I stand a MUCH BETTER chance of figuring out close relatives using the combined closest matches of all four databases instead of the top matches from just one database. It doesn’t matter if the database is large if the right person or people didn’t test there.

Combine Resources

I’ll be providing analysis methodologies for working with results from all of the vendors together, just in case your answer is not immediately obvious. Taking multiple DNA tests facilitates using all of these tools immediately, not months later. Solving the puzzle sooner means you may not miss valuable opportunities.

You may also discover that the door slams shut with some people, or they may not respond to your queries, but another match may be unbelievably helpful. Don’t limit your possibilities.

Let’s take a look at the strengths of each vendor.

Vendor Strengths and Things to Know

Every vendor has product strengths and idiosyncracies that the others do not. All vendors provide matches and shared matches. Each vendor provides ethnicity tools which certainly can be useful, but the features differ and will be covered elsewhere.

  • Ancestry – Ancestry has the largest autosomal database and includes ThruLines, but no Y or mitochondrial DNA testing, no clusters, no chromosome browser, no triangulation, and no X chromosome matching or reporting. Ancestry provides genealogical records, advanced tools, and full tree access to your matches’ trees with an Ancestry subscription. Ancestry does not allow downloading your match list or segment match information, but the other vendors do.
  • 23andMe – 23andMe has the second largest database. They provide triangulation and genetic trees that include your closest matches. Many people test at 23andMe for health and wellness information, so 23andMe has people in their database who are not specifically interested in genealogy and probably won’t have tested elsewhere, but may be invaluable to your search. 23andMe provides Y and mtDNA high-level haplogroups only, but no matching or other haplogroup information. If you purchase a new test or have a V5 ancestry+health current test, you can expand your matches from a limit of 1500 to about 5000 with an annual membership. For seeking close relatives, you don’t need those features, but you may want them for genealogy. 23andMe is the only vendor that limits their customers’ matches.
  • MyHeritage – MyHeritage has the third largest database that includes lots of European testers. MyHeritage provides triangulation, Theories of Family Relativity, and an integrated cluster tool* but does not report X matches and does not offer Y or mitochondrial DNA testing. MyHeritage accepts autosomal DNA file uploads from other testing companies for free and provides access to advanced DNA features for a one-time unlock fee. MyHeritage includes genealogical records and full feature access to advanced DNA tools with a Complete Subscription. (Free 15 days trial subscription, here.)
  • FamilyTreeDNA Family Finder (autosomal) – FamilyTreeDNA is the oldest DNA testing company, meaning their database includes people who initially tested 20+ years ago and have since passed away. This, in essence, gets you one generation further back in time, with the possibility of stronger matches. Their Family Matching feature buckets and triangulates your matches, assigning them to your maternal or paternal sides if you link known matches to their proper place in your tree, even if your parents have not tested. FamilyTreeDNA accepts uploads from other testing companies for free and provides advanced DNA features for a one time unlock fee.
  • FamilyTreeDNA – FamilyTreeDNA is the only company that offers both Y and mitochondrial DNA testing products that include matching, integration with autosomal test results, and other tools. These two tests are lineage-specific and don’t have to be sorted from your other ancestral lines.

I wrote about using Y DNA results, here.

I wrote about using mitochondrial DNA results, here.

*Third parties such as Genetic Affairs provide clustering tools for both 23andMe and FamilyTreeDNA. Clustering is integrated at MyHeritage. Ancestry does not provide a tool for nor allow third-party clustering. If the answer you seek isn’t immediately evident, Genetic Affairs clustering tools group people together who are related to each other, and you, and create both genetic and genealogical trees based on shared matches. You can read more about their tools, here.

Fish in all the Ponds and Use All the Bait Possible

Here’s the testing and upload strategy I recommend, based on the above discussion and considerations. The bottom line is this – if you want as much information as possible, as quickly as possible, order the four tests in red initially. Then transfer the first autosomal test results you receive to the two companies identified in blue. Optionally, GEDMatch may have tools you want to work with, but they aren’t a testing company.

What When Ancestry 23andMe MyHeritage FamilyTreeDNA
Order autosomal Initially X X    
Order Y 111 or Big-Y DNA test if male Initially       X
Order mitochondrial DNA test Initially if desired       X
Upload free autosomal When Ancestry or 23andMe results are available     X X
Unlock Advanced Tools When you upload     $29 $19
Optional GEDMatch free upload If desired, can subscribe for advanced tools

When you upload an autosomal DNA file to a vendor site, only upload one file per site, per tester. Otherwise, multiple tests simply glom up everyone’s match list with multiple matches to the same person.

Multiple vendor sites will hopefully provide multiple close matches, which increase your opportunity to discover INFORMATION about your family, not just the identity of the person you seek.

Or maybe you prefer to wait and order these DNA tests serially, waiting until one set of results is back and you’re finished working with them before ordering the next one. If so, that means you’re a MUCH more patient person than me. 😊

Our next article in this series will be about endogamy, how to know if it applies to you, and what that means to your search.

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DNA: In Search of…What Do You Mean I’m Not Related to My Family? – and What Comes Next?

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Welcome to the second in our series of articles about how to search for unknown family members.

I introduced the series in the article, DNA: In Search of…New Series Launches.

This article addresses the question of “How did this happen?” and introduces the tools we need to answer that question. I’ve combined two articles into one because I really didn’t want to leave you hanging after introducing you to the problem.

We discuss the various kinds of DNA tests, when they are appropriate for your biological sex, and how one can use them to discover information about the person or people you’re seeking.

In other words, we begin at the point of making the discovery that there is something amiss, then review possible glitches. Once we confirm there is someone you need to search for, we discuss how to use genetic testing reasonably and in a planned fashion to solve that mystery.

Please note that I am NOT referring to unexpected ethnicity results in this article. This article refers to your match list and who you do and don’t match on that list. We will discuss ethnicity and how it can help you in a different context in a future article.

The Unknown

Some people have known all their lives that they were adopted, or that they didn’t know the identity of one parent, generally their father.

Other people have made or will make that discovery in a different way. Sometimes, that realization happens when they take an autosomal DNA test and don’t match people they expect to match, either not at all or in a different way.

For example:

  • You might not match a parent or a sibling.
  • You could match only people on your mother’s side, but no known relatives on your father’s side.
  • Your parents or siblings have tested, but you don’t match any of them.
  • Your immediate family hasn’t tested, but your first and second cousins have tested, and you don’t match any of them.
  • You recognize no people, families, or family names on your match list.
  • You think you know your genealogy, but nothing on your match list looks familiar.
  • If your parents and close relatives haven’t tested, not recognizing families might be explained if your family is part of a community of undertested individuals.
  • You might not recognize anyone or surnames if you know absolutely nothing about your family genealogy.
  • Sometimes, a sibling is reported as a half-sibling instead of a full sibling, which is an unexpected finding. This means that you only share one parent, not two. I wrote about this in the article Full or Half Siblings. The non-matching parent is generally the father. The question that follows is, which one of you, if not both, weren’t fathered by the man you thought was your biological father?

These discoveries are generally unexpected and unwelcome – a horrible shock followed by some level of disbelief.

I’ve been there.

My half-brother turned out to not be my half-brother, so we weren’t biologically related at all, although that didn’t change how much I loved him one iota.

Later, I did identify his father, but it was too late for them. My brother had passed on by that time.

Ironically, his biological family would have welcomed him with open arms.

If you’re interested, I wrote about our journey in a series of articles:

The Shock of Discovery

It’s difficult discovering that your full sibling isn’t a full sibling or not a sibling at all, but it’s even worse when you discover that one or both of your parents are not your biological parent(s) when you weren’t expecting that. Obviously, sometimes those two shockers accompany each other.

And no, if you don’t match your parents, siblings, first or second cousins, DNA tests can’t be “that” wrong in terms of matching. That’s generally the first question everyone asks.

Yes, we have seen a couple of instances of test mix-ups at the labs, many years ago, among the millions of tests taken. Better quality control procedures were introduced, and a mix-up hasn’t happened in a very long time. However, if you really think that’s a possibility, or you need peace of mind – order another test from the same vendor. If the second test comes back with the same match list as the first test, there is no lab mix-up.

Or, you can order a test from another vendor – something you’re going to need anyway to solve the mystery and for your genealogy. Hint – the two vendors you must test at directly are Ancestry and 23andMe because they don’t accept uploads. If you’re going to order another test, make it one or both of those.

Before deciding you’ve discovered a genetic disconnect, let’s take a deep breath and look at a couple of other possibilities first.

Be Sure the Vial or Transfer Wasn’t Confused

If you’re encountering a situation where you’re not matching relatives that you know have tested, or for some reason, you suspect something isn’t right, the first things that need to be considered are:

  • Are you positive that your relative(s) have taken a DNA test? You wouldn’t believe how many times someone has told me that they don’t match their mother/father/sibling and come to find out, their family member hasn’t tested. Did they order a test but never send it in? Did they send it in, but their results arent’ back yet?
  • Are you positive that your relative(s) tested at the same company where you did? Many times we discover that they’ve tested, but at a different company. Have your relative show you their results, take a screenshot, or give you their login to confirm you’re at the same vendor.
  • Are you missing all of your relatives or just one or two in the same line? If the answer is one or two, they, not you, may have a disconnect, especially if you match other people on the same side of your family.
  • Did you and a friend or spouse both swab or spit at the same time? If so, is there any possibility that your and their vials were inadvertently swapped when you put them in envelopes and mailed them?

If there is any doubt, check with that other person and see if they are experiencing the same issue. If you look at their results, you may recognize your own family. I’ve seen this occur at family reunions and at the holidays, where several DNA tests were taken by various family members.

  • This last situation is much more common and is caused by confusing files during a download/upload to another vendor. Do you manage multiple kits, and did you inadvertently download the wrong DNA file, or upload the wrong person’s DNA file to a different vendor?

If so, you’re looking at someone else’s results, thinking they are your own. If that person is a cousin, you may be even more confused because you may match some of the same people, just at very different levels. This could make your sibling look like a half-sibling or first cousin, for example.

If there is any possibility of an upload mix-up, or any doubt whatsoever:

  1. Delete the suspect file at the vendor where you uploaded the DNA file
  2. Delete the downloaded files from your computer
  3. Start over by downloading the DNA file again from the original vendor
  4. Label the downloaded file clearly, and immediately, with the tester’s name and date.
  5. Upload the new file to the target vendor before you download another person’s DNA file.

Step-by-step upload/download instructions can be found, here.

Not Parent Expected

If you discover that one of two parents is not the expected biological parent, you’ve discovered a genetic disconnect that is known by a number of different terms. Initially, the term NPE was used, but other terms have been added over the years, and they are sometimes used differently, depending on who is speaking.

  • NPE – Non-Parental Event, Not Parent Expected
  • MPE – Misattributed Paternal/Parental Event or Misattributed Parentage Experience
  • Undocumented Adoption – Regardless of how the situation occurred, it was not documented.

Please, please do NOT jump to conclusions and make assumptions about infidelity and duplicity. There can be many reasons for this occurrence, including:

  • Agreed upon “open” relationships
  • Intentional impregnation when one partner is infertile
  • Surrogacy
  • Infidelity
  • Rape
  • Sperm donor
  • Adoption
  • Unknown first marriage, with step-father raising a child as his own
  • Illegitimate birth of a child before marriage
  • Lifestyle choices
  • Intoxication
  • Coercion

In other words, the situation may have been known to the involved parties, even if they did not share that information with you or others. Prior to the last 20 years, no one would ever have considered that this information might ever be revealed. Social norms and judgments were very different a generation or more ago.

I wrote about this in the article, Things That Need To Be said: Adoption, Adultery, Coercion, Rape, and DNA.

Of course, these events could happen in any generation, but the closer to you, in time, the more evident it will be when looking at your matches.

Now that we’ve determined that we have an unknown parent or grandparent, how do we sort this out?

Let’s Start with the Basics

I’m going to begin by explaining the basics of the different kinds of tests, and when each test can be used.

In this series, we will be focused on searching for six individuals, separately – both parents and all four grandparents.

You will be able to use the same techniques for ancestors in more distant generations by following the same instructions and methodologies, just adapting to include more matches to reach further back in time.

We will be taking the search step-by-step in each article.

Four Kinds of DNA

For genealogy, we can work with four kinds of DNA:

We can potentially use each of these when searching for unknown ancestors, including parents and grandparents. Each type of DNA has specific characteristics and uses in different situations because it’s inherited differently by the son and daughter, below.

In these examples, everything is from the perspective of the son and daughter.

Y DNA testing is only available to males, because only males have a Y chromosome which is inherited directly from the father, shown by the blue arrow. In other words, the son has the father’s Y chromosome (and generally his surname,) but the daughter does not.

The Y chromosome can provide surnames and very close matches, or reach far back in time, or both. Ideally, Y DNA is used in conjunction with autosomal testing when searching for unknown individuals.

Mitochondrial DNA can be tested by everyone since males and females both receive mitochondrial DNA from their mother, passed to her from her direct maternal line, shown by the pink arrows and the yellow hearts. Both the son and daughter can test for their mother’s mitochondrial DNA.

Both Y DNA and mitochondrial DNA can reach far back in time, but can also be informative of recent connections. Neither are ever mixed with the DNA of the other parent, so the DNA is not diluted over the generations.

Think of Y DNA and mitochondrial DNA as having the ability to provide recent genealogy information and connections, plus a deep dive on just one particular line. Fortunately, when you’re looking for parents, the lines they test are the direct maternal (or matrilineal) line and the direct paternal (or patrilineal) lines.

Both Y DNA and mitochondrial DNA tests are deep, not broad. One line each.

Y DNA and mitochondrial DNA will both be able to tell you if that specific ancestral line is European, African, Native American, Asian, Jewish, and so forth. Additionally, both offer matching at FamilyTreeDNA, information about where other testers’ ancestors are found in the world, and more.

If you want more information about what these tests have to offer, now, I provide a Y DNA Resource page, here, and a Mitochondrial DNA Resource page, here.

Autosomal DNA is the DNA contributed to you on chromosomes 1-22 by your ancestors from across all your ancestral lines in your tree, shown by the green arrow.

Everyone receives half of their autosomal DNA from each parent, with the exception of the X chromosome, which we’ll discuss in a minute.

This means that because the parent’s DNA is cut in half in each generation, the contributions of more distant ancestors’ DNA are reduced over time, with each generational division, until it’s no longer discernable or disappears altogether.

Autosomal DNA is broad across many lines, but not deep.

This figure provided by Dr. Paul Maier at FamilyTreeDNA, in the MyOrigins 3.0 White Paper, illustrates that by the 7th generation, you won’t receive DNA from a few of your ancestors. Some may be contained in segments too small to be reported by DNA testing vendors.

Translated, this means that autosomal DNA matching is most reliable in the closest generations, which is where we are working.

There is no documented occurrence of second cousins who don’t match each other. 90% of third cousins match, and about 50% of fourth cousins. I wrote about that in the article, Why Don’t I Match My Cousin?

The 23rd Chromosome – Sex Determination

Autosomal DNA generally refers to chromosomes 1-22. The 23rd chromosome is the sex selection chromosome.

Males have a Y chromosome contributed by their father, and an X contributed by their mother. The Y chromosome is what makes males, male.

Females have an X chromosome contributed by both their mother and father, which recombines just like chromosomes 1-22, but women have no Y chromosome.

In this graphic, you can see that a male child receives the father’s Y chromosome and the mother’s X. The female child receives an X chromosome from both parents.

Only FamilyTreeDNA and 23andMe report X chromosome results by including them with their autosomal DNA test.

Let’s take a look at how the X chromosome works in a little more detail.

X Chromosome DNA is another type of autosomal DNA, meaning it can be inherited from both parents in some circumstances. However, the X chromosome has a different inheritance path which means we analyze it differently for genealogy.

The father gives an X or a Y chromosome to his offspring, but not both.

If the child inherits the Y chromosome from the father, the child becomes a male. If the child inherits the X chromosome from the father, the child becomes a female.

Men only receive an X chromosome from their mother since they receive a Y chromosome from their father. Men can inherit a mixture of their mother’s X chromosomes that were contributed to their mother from both her mother (peach) and father (green.) Conversely, men can inherit their maternal grandmother’s or maternal grandfather’s X chromosome intact.

In this example, the mother and father have three sons. None of the sons can inherit an X chromosome from their father, whose X chromosome is shown in yellow. The father gives the sons his Y chromosome, not shown here, instead of an X, which is how they become males. Males only inherit their X chromosome from their mother.

The mother inherited one copy of her X chromosome from her father, shown in green, and one copy from her mother, shown in peach.

  1. The first son inherited his maternal grandfather’s green X chromosome, intact, from his mother, and none of his maternal grandmother’s peach X chromosome.
  2. The second son inherited a portion of his maternal grandmother’s peach X chromosome and a portion of his maternal grandfather’s green X chromosome. I’ve shown the portions as half, but the division could vary.
  3. The third son inherited his maternal grandmother’s peach X chromosome, intact, and none of his maternal grandfather’s green X chromosome.

This means if you match a man on his X chromosome, assuming it’s a valid match and not identical by chance, that match MUST come from his mother’s line.

In a future article, I’ll provide some X-specific fan charts and tips to help you easily discern potential X inheritance paths.

Women inherit an X chromosome from both their mother and father. They inherit their father’s X chromosome intact that he received from his mother, because he only has one X to give his daughter. Therefore, daughters inherit their paternal grandmother’s X chromosome from their father, because he passes on exactly what he received from his mother.

In this graphic, the father and mother have three daughters. You can see that each daughter receives the father’s yellow X chromosome that he inherited from his mother.

He doesn’t have a second copy of an X chromosome to mix with his mother’s.

Women inherit their mother’s X chromosome in the same fashion that men do. You can see in our example that:

  • The first daughter inherited her father’s yellow X chromosome, plus her maternal grandmother’s peach X chromosome, intact, and none of her maternal grandfather’s green X chromosome.
  • The second daughter inherited her father’s yellow X chromosome, plus part of her maternal grandfather’s green X chromosome and part of her maternal grandmother’s peach X chromosome from her mother. The portions of the mother’s pink and green chromosomes inherited by the daughter can vary widely.
  • The third daughter inherited her father’s yellow X chromosome, plus her maternal grandfather’s green X chromosome, intact, which is his mother’s X chromosome, of course. This daughter inherited none of her maternal grandmother’s peach X chromosome.

Women inherit two X chromosomes, one from each parent, while men only inherit one X, contributed from their mother. This means that X matches have different inheritance paths for women and men.

Because the X inheritance path involves the mother, many people confuse mitochondrial DNA inheritance with X inheritance. I wrote about that in the article, X Matching and Mitochondrial DNA is NOT the Same Thing.

Testing Strategies and Vendor Strengths

In the next article, we will be discussing detailed testing strategies based on multiple factors:

  • Who you are searching for in your tree
  • Who, other than you, is available to test
  • Sex of the tester(s)
  • Vendor strengths and unique offerings
  • Urgency, or not
  • Using combinations of vendor results and why you want to

Getting lucky may be what you hope for, but it’s not a strategy.😊

_____________________________________________________________

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DNA-eXplained Celebrates Tenth Anniversary!

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This blog, DNA-eXplained, is celebrating its 10th anniversary today. How time flies!

I never thought for a minute about a 10th anniversary when I launched that first article.

I started blogging to teach people and literally “explain” about genetic genealogy – which is why I selected the name DNA-eXplained. Over time, it has also been nicknamed DNAeXplain, which is fine.

I hoped to be able to answer questions once, with graphics and examples, instead of over and over again off-the-cuff. I needed someplace where people could be referred for answers. Blogging seemed like the perfect medium for achieving exactly that.

Blogs allow writers to publish content attractively and react to changes and announcements quickly.

Blogs encourage readers to subscribe for email delivery or use RSS reader aggregation and can publish to social media.

Content can be located easily using browser searches.

Everything, all content, is indexed and searchable by keyword or phrase.

Blogging certainly seemed like the right solution. Still, I was hesitant.

I vividly remember working at my desk that day, a different desk in a different location, and anguishing before pressing the “publish” button that first time. Was I really, REALLY sure? I had the sense that I was sitting in one of those life-defining fork-in-the-road moments and once embarked upon, there would be no turning back.

I’m so glad I closed my eyes and pushed that button!

I knew we were going to be in for an incredible journey. Of course, I had no idea where that roller coaster ride was going, but we would be riding together, regardless. What a journey it has been!

A decade later, I’ve had the opportunity to meet and become friends with so many of you, both online and in person. I’ve met countless cousins I never knew I had, thanks to various blog articles, including the 52 Ancestors series which has turned out to be 365 and counting.

I am incredibly grateful for this opportunity! I thought I was giving to others, yet I’ve been greatly enriched by this experience and all of you.

So much has changed in all of our lives.

Looking Back

Today, as I look back at that very short first article, I can’t help but think just how unbelievably far we’ve come.

There was one Y and mitochondrial DNA testing vendor in 2012, FamilyTreeDNA, and that’s still the case today.

There were three autosomal testing companies, 23andMe, FamilyTreeDNA, and Ancestry, in addition to the Genographic Project, which was sunset in 2019 after an amazing 15-year run. GEDmatch was two years old in 2012 and had been formed to fill the need for advanced autosomal matching tools. In 2016, MyHeritage joined the autosomal testing market. All of those companies have since been acquired.

In 2012, FamilyTreeDNA broke ground by accepting uploaded DNA files from other vendors. Autosomal DNA tests cost about $300 although prices were dropping. I don’t anticipate prices dropping much further now, because companies have to maintain a reasonable profit margin to stay in business.

In 2013, when DNA-eXplained celebrated its first anniversary, I had published 162 articles.

That first year was VERY busy with lots of innovation occurring in the industry. You can read my end-of-year article, 2012 Top 10 Genetic Genealogy Happenings if you’d like to reminisce a bit. For comparison, here’s my Genetic Genealogy at 20 Years summary.

The World is Our Oyster

In the past decade, I’ve penned articles in a wide variety of locations, in several countries, on 5 continents.

I’ve written in my offices, of course, but also in cars, on buses, trains, and planes. I’ve crafted several articles on ships while cruising. In fact, writing is one of my favorite “sea-day” things to do, often sitting on deck if it’s a nice day.

I’ve written in cemeteries, which shouldn’t surprise you, on the hood of my car, and cross-legged on the floor at innumerable conferences.

I’ve composed at picnic tables and in countless hotel lobbies, libraries, laboratories, restaurants, and coffee shops. And, in at least 3 castles.

I’ve written while on archaeology digs, balancing my laptop on my knees while sitting on an inverted bucket, trying to keep dirt, sand, and ever-present insects away.

I’ve even written in hospitals, both as a visitor and a patient. Yea, I might not have told you about that.

I’ve pretty much taken you with me everyplace I’ve gone for the past decade. And we are no place near finished!

Today

This article is number 1531 which means I’ve published an article every 2.3 days for a decade. Truthfully, I’m stunned. I had no idea that I have been that prolific. I never have writer’s block. In fact, I have the opposite problem. So many wonderful topics to write about and never enough time.

A huge, HUGE thank you to all of my readers. Writers don’t write if people don’t read!

DNA-eXplained has received millions and millions of views and is very popular, thanks to all of you.

There have been more than 48,000 comments, 4,800 a year or about 13 each day, and yes, I read every single one before approving it for publication.

Akismet, my spam blocker only reports for 45 months, but in that time alone, there have been about 100,000 attempted SPAM comments. That equates to about 75 each day and THANK GOODNESS I don’t have to deal with those.

WordPress doesn’t count “pages,” as such, but if my articles average 10 pages each, and each page averages 500 words, then we’re looking at someplace between 7 and 8 million words. That’s 13 times the size of War and Peace😊. Not only do I write each article, but I proofread it several times too.

Peering Into the Future

Genetic genealogy as a whole continues to produce the unexpected and solve mysteries.

Tools like triangulation in general, Family Matching at FamilyTreeDNA, genetic trees at 23andMe, Theories of Family Relativity at MyHeritage, and ThruLines at Ancestry have provided hints and tools to both suggest and confirm relationships and break through brick walls.

Ethnicity chromosome painting at both 23andMe and FamilyTreeDNA help unravel ancestral mysteries, especially for people with combinations of fundamentally different ancestries, as does Genetic Communities at Ancestry and Genetic Groups at MyHeritage.

Third-party tools that we love today weren’t even a twinkle in a developer’s eye in 2012. Products like DNAPainter, Genetic Affairs, and DNAGedcom pick up where the vendors leave off and are widely utilized by genealogists.

I hope that all of our vendors continue to invest in product development and provide the genetic genealogy community with new and innovative tools that assist us with breaking down those pesky brick walls.

Primarily, though, I hope you continue to enjoy your genealogy journey and make steady progress, with a rocket boost from genetic testing.

The vendors can provide wonderful tools, but it’s up to us to use them consistently, wringing out every possible drop. Don’t neglect paternal (male surname) Y DNA and matrilineal mitochondrial DNA testing for people who carry those important lines for your ancestors. All 4 kinds of DNA have a very specific and unique genealogical use.

I encourage you to test every relative you can and check their and your results often. New people test every single day. You never know where that critical piece of information will come from, or when that essential puzzle piece will drop into place.

Be sure to upload to both FamilyTreeDNA and MyHeritage (plus GEDMatch) so you are in the database of all the vendors. (Instructions here.) Fate favors the prepared.

Thank You!!

Thank you from the bottom of my heart for supporting me by reading and sharing my articles with your friends, organizations, and family members, by purchasing through the affiliate links, by buying my book, and by graciously sharing your own experiences.

Thank you for your suggestions and questions which plant the seeds of new articles and improvements.

I hope you’ve made progress with your research, unraveled some thorny knots, and that you’ve enjoyed this decade as much as I have. Tell me in the comments what you enjoyed the most or found most useful?

Here’s to another wonderful 10 years together!

___________________________________________________________

Follow DNAexplain on Facebook, here or follow me on Twitter, here.

Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an email whenever I publish by clicking the “follow” button on the main blog page, here.

You Can Help Keep This Blog Free

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Uploads

Genealogy Products and Services

My Book

Genealogy Books

Genealogy Research

 

FamilyTreeDNA DISCOVER™ Launches – Including Y DNA Haplogroup Ages

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FamilyTreeDNA just released an amazing new group of public Y DNA tools.

Yes, a group of tools – not just one.

The new Discover tools, which you can access here, aren’t just for people who have tested at FamilyTreeDNA . You don’t need an account and it’s free for everyone. All you need is a Y DNA haplogroup – from any source.

I’m going to introduce each tool briefly because you’re going to want to run right over and try Discover for yourself. In fact, you might follow along with this article.

Y DNA Haplogroup Aging

The new Discover page provides seven beta tools, including Y DNA haplogroup aging.

Haplogroup aging is THE single most requested feature – and it’s here!

Discover also scales for mobile devices.

Free Beta Tool

Beta means that FamilyTreeDNA is seeking your feedback to determine which of these tools will be incorporated into their regular product, so expect a survey.

If you’d like changes or something additional, please let FamilyTreeDNA know via the survey, their support line, email or Chat function.

OK, let’s get started!

Enter Your Haplogroup

Enter your Y DNA haplogroup, or the haplogroup you’re interested in viewing.

If you’re a male who has tested with FamilyTreeDNA , sign on to your home page and locate your haplogroup badge at the lower right corner.

If you’re a female, you may be able to test a male relative or find a haplogroup relevant to your genealogy by visiting your surname group project page to locate the haplogroup for your ancestor.

I’ll use one of my genealogy lines as an example.

In this case, several Y DNA testers appear under my ancestor, James Crumley, in the Crumley DNA project.

Within this group of testers, we have two different Big Y haplogroups, and several estimated haplogroups from testers who have not upgraded to the Big Y.

If you’re a male who has tested at either 23andMe or LivingDNA, you can enter your Y DNA haplogroup from that source as well. Those vendors provide high-level haplogroups.

The great thing about the new Discover tool is that no matter what haplogroup you enter, there’s something for you to enjoy.

I’m going to use haplogroup I-FT272214, the haplogroup of my ancestor, James Crumley, confirmed through multiple descendants. His son John’s descendants carry haplogroup I-BY165368 in addition to I-FT272214, which is why there are two detailed haplogroups displayed for this grouping within the Crumley haplogroup project, in addition to the less-refined I-M223.

Getting Started

When you click on Discover, you’ll be asked to register briefly, agree to terms, and provide your email address.

Click “View my report” and your haplogroup report will appear.

Y DNA Haplogroup Report

For any haplogroup you enter, you’ll receive a haplogroup report that includes 7 separate pages, shown by tabs at the top of your report.

Click any image to enlarge

The first page you’ll see is the Haplogroup Report.

On the first page, you’ll find Haplogroup aging. The TMRCA (time to most recent common ancestor) is provided, plus more!

The report says that haplogroup I-FT272214 was “born,” meaning the mutation that defines this haplogroup, occurred about 300 years ago, plus or minus 150 years.

James Crumley was born about 1710. We know his sons carry haplogroup I-FT272214, but we don’t know when that mutation occurred because we don’t have upstream testers. We don’t know who his parents were.

Three hundred years before the birth of our Crumley tester would be about 1670, so roughly James Crumley’s father’s generation, which makes sense.

James’ son John’s descendants have an additional mutation, so that makes sense too. SNP mutations are known to occur approximately every 80 years, on average. Of course, you know what average means…may not fit any specific situation exactly.

The next upstream haplogroup is I-BY100549 which occurred roughly 500 years ago, plus or minus 150 years. (Hint – if you want to view a haplogroup report for this upstream haplogroup, just click on the haplogroup name.)

There are 5 SNP confirmed descendants of haplogroup I-FT272214 claiming origins in England, all of whom are in the Crumley DNA project.

Haplogroup descendants mean this haplogroup and any other haplogroups formed on the tree beneath this haplogroup.

Share

If you scroll down a bit, you can see the share button on each page. If you think this is fun, you can share through a variety of social media resources, email, or copy the link.

Sharing is a good way to get family members and others interested in both genealogy and genetic genealogy. Light the spark!

I’m going to be sharing with collaborative family genealogy groups on Facebook and Twitter. I can also share with people who may not be genealogists, but who will think these findings are interesting.

If you keep scrolling under the share button or click on “Discover More” you can order Y DNA tests if you’re a biological male and haven’t already taken one. The more refined your haplogroup, the more relevant your information will be on the Discover page as well as on your personal page.

Scrolling even further down provides information about methods and sources.

Country Frequency

The next tab is Country Frequency showing the locations where testers with this haplogroup indicate that their earliest known ancestors are found.

The Crumley haplogroup has only 5 people, which is less than 1% of the people with ancestors from England.

However, taking a look at haplogroup R-M222 with many more testers, we see something a bit different.

Ireland is where R-M222 is found most frequently. 17% of the men who report their ancestors are from Ireland belong to haplogroup R-M222.

Note that this percentage also includes haplogroups downstream of haplogroup R-M222.

Mousing over any other location provides that same information for that area as well.

Seeing where the ancestors of your haplogroup matches are from can be extremely informative. The more refined your haplogroup, the more useful these tools will be for you. Big Y testers will benefit the most.

Notable Connections

On the next page, you’ll discover which notable people have haplogroups either close to you…or maybe quite distant.

Your first Notable Connection will be the one closest to your haplogroup that FamilyTreeDNA was able to identify in their database. In some cases, the individual has tested, but in many cases, descendants of a common ancestor tested.

In this case, Bill Gates is our closest notable person. Our common haplogroup, meaning the intersection of Bill Gates’s haplogroup and my Crumley cousin’s haplogroup is I-L1195. The SNP mutation that defines haplogroup I-L1145 occurred about 4600 years ago. Both my Crumley cousin and Bill Gates descend from that man.

If you’re curious and want to learn more about your common haplogroup, remember, you can enter that haplogroup into the Discover tool. Kind of like genetic time travel. But let’s finish this one first.

Remember that CE means current era, or the number of years since the year “zero,” which doesn’t technically exist but functions as the beginning of the current era. Bill Gates was born in 1955 CE

BCE means “before current era,” meaning the number of years before the year “zero.” So 2600 BCE is approximately 4600 years ago.

Click through each dot for a fun look at who you’re “related to” and how distantly.

This tool is just for fun and reinforces the fact that at some level, we’re all related to each other.

Maybe you’re aware of more notables that could be added to the Discover pages.

Migration Map

The next tab provides brand spanking new migration maps that show the exodus of the various haplogroups out of Africa, through the Middle East, and in this case, into Europe.

Additionally, the little shovel icons show the ancient DNA sites that date to the haplogroup age for the haplogroup shown on the map, or younger. In our case, that’s haplogroup I-M223 (red arrow) that was formed about 16,000 years ago in Europe, near the red circle, at left. These haplogroup ancient sites (shovels) would all date to 16,000 years ago or younger, meaning they lived between 16,000 years ago and now.

Click to enlarge

By clicking on a shovel icon, more information is provided. It’s very interesting that I-L1145, the common haplogroup with Bill Gates is found in ancient DNA in Cardiff, Wales.

This is getting VERY interesting. Let’s look at the rest of the Ancient Connections.

Ancient Connections

Our closest Ancient Connection in time is Gen Scot 24 (so name in an academic paper) who lived in the Western Isles of Scotland.

These ancient connections are more likely cousins than direct ancestors, but of course, we can’t say for sure. We do know that the first man to develop haplogroup I-L126, about 2500 years ago, is an ancestor to both Gen Scot 24 and our Crumley ancestor.

Gen Scot 24 has been dated to 1445-1268 BCE which is about 3400 years ago, which could actually be older than the haplogroup age. Remember that both dating types are ranges, carbon dating is not 100% accurate, and ancient DNA can be difficult to sequence. Haplogroup ages are refined as more branches are discovered and the tree grows.

The convergence of these different technologies in a way that allows us to view the past in the context of our ancestors is truly amazing.

All of our Crumley cousin’s ancient relatives are found in Ireland or Scotland with the exception of the one found in Wales. I think, between this information and the haplogroup formation dates, it’s safe to say that our Crumley ancestors have been in either Scotland or Ireland for the past 4600 years, at least. And someone took a side trip to Wales, probably settled and died there.

Of course, now I need to research what was happening in Ireland and Scotland 4600 years ago because I know my ancestors were involved.

Suggested Projects

I’m EXTREMELY pleased to see suggested projects for this haplogroup based on which projects haplogroup members have joined.

You can click on any of the panels to read more about the project. Remember that not everyone joins a project because of their Y DNA line. Many projects accept people who are autosomally related or descend from the family through the mitochondrial line, the direct mother’s line.

Still, seeing the Crumley surname project would be a great “hint” all by itself if I didn’t already have that information.

Scientific Details

The Scientific Details page actually has three tabs.

The first tab is Age Estimate.

The Age Estimate tab provides more information about the haplogroup age or TMRCA (Time to Most Recent Common Ancestor) calculations. For haplogroup I-FT272214, the most likely creation date, meaning when the SNP occurred, is about 1709, which just happens to align well with the birth of James Crumley about 1710.

However, anyplace in the dark blue band would fall within a 68% confidence interval (CI). That would put the most likely years that the haplogroup-defining SNP mutation took place between 1634 and 1773. At the lower end of the frequency spectrum, there’s a 99% likelihood that the common ancestor was born between 1451 and 1874. That means we’re 99% certain that the haplogroup defining SNP occurred between those dates. The broader the date range, the more certain we can be that the results fall into that range.

The next page, Variants, provides the “normal” or ancestral variant and the derived or mutated variant or SNP (Single Nucleotide Polymorphism) in the position that defines haplogroup I-FT272214.

The third tab displays FamilyTreeDNA‘s public Y DNA Tree with this haplogroup highlighted. On the tree, we can see this haplogroup, downstream haplogroups as well as upstream, along with their country flags.

Your Personal Page

If you have already taken a DNA test at FamilyTreeDNA, you can find the new Discover tool conveniently located under “Additional Tests and Tools.”

If you are a male and haven’t yet tested, then you’ll want to order a Y DNA test or upgrade to the Big Y for the most refined haplogroup possible.

Big Y tests and testers are why the Y DNA tree now has more than 50,000 branches and 460,000 variants. Testing fuels growth and growth fuels new tools and possibilities for genealogists.

What Do You Think?

Do you like these tools?

What have you learned? Have you shared this with your family members? What did they have to say? Maybe we can get Uncle Charley interested after all!

Let me know how you’re using these tools and how they are helping you interpret your Y DNA results and assist your genealogy.

_____________________________________________________________

Follow DNAexplain on Facebook, here or follow me on Twitter, here.

Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an email whenever I publish by clicking the “follow” button on the main blog page, here.

You Can Help Keep This Blog Free

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Uploads

Genealogy Products and Services

My Book

Genealogy Books

Genealogy Research

Just Released – Mitochondrial Haplogroup L7 Video!

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I’m still VERY excited about the haplogroup L7 discovery. Mitochondrial Eve’s new 100,000-year-old great-granddaughter. So is the rest of the Million Mito Team

We’ve created a short video explaining just why this is so cool.

Paul, Dr. Maier, the Population Geneticist on our Million Mito team did a great job as producer. He’s certainly multi-talented! Thanks Paul.

Please understand that this is “just us,” no professional production, editors or anything like that. You’re seeing the real deal here. This video is something we wanted to do for all of you. We’re excited to tell this amazing story – one that we’ve explained in terms that everyone can understand and enjoy. We want you to love mitochondrial DNA as much as we do.

Please share this video far and wide with your family and friends. Remind them that everyone inherits their mother’s (and only their mother’s) mitochondrial DNA. They can make cool discoveries too.

But wait, there’s more!

Dr. Miguel Vilar’s Article

FamilyTreeDNA just published a guest blog article titled A 100,000Year-Old Human Lineage Rediscovered, written by genetic anthropologist Dr. Miguel Villar.

You’ll recognize Miguel as one of the four Million Mito team members in the video, but you may also remember him as the Senior Program Officer for the National Geographic Society and the Lead Scientist for the Genographic Project.

I think you’ll agree, he’s a great writer too!

What’s Your Story?

Not only is mitochondrial DNA (mtDNA) useful genealogically, it’s the story of all womankind. You don’t have to be a genealogist to appreciate and enjoy your mtDNA journey.

Mitochondrial DNA tells a story about each of us that we would never know otherwise.

The best part is that every single person can test their own mitochondrial DNA to learn more about their family story – and very specifically about their mother’s direct line ancestry that may be eclipsed or overshadowed in autosomal DNA by more recent admixture.

Where does your mitochondrial DNA lead?

What Else Can You Do?

You, your mother, and your maternal siblings all share the same mitochondrial DNA, passed to you by your mother. But what about your father? He inherited HIS mother’s mitochondrial DNA, but you didn’t.

You can discover your paternal grandmother’s mtDNA story by testing your father’s mtDNA, or his maternal line siblings if he’s not available for testing.

Your paternal grandmother’s story is your family story too!

Let me know if you like the video and if it makes mtDNA easier to understand and explain to your relatives. I hope this discovery and video help sew the seeds of curiosity.

_____________________________________________________________

Follow DNAexplain on Facebook, here or follow me on Twitter, here.

Share the Love!

You’re always welcome to forward articles or links to friends and share on social media.

If you haven’t already subscribed (it’s free,) you can receive an email whenever I publish by clicking the “follow” button on the main blog page, here.

You Can Help Keep This Blog Free

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Uploads

Genealogy Products and Services

My Book

Genealogy Books

Genealogy Research

Mitochondrial Eve Gets a Great-Granddaughter: African Mitochondrial Haplogroup L7 Discovered

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Such wonderful news today!

We have a birth announcement, of sorts, detailed in our new paper released just today,  “African mitochondrial haplogroup L7: a 100,000-year-old maternal human lineage discovered through reassessment and new sequencing.”

Woohoo, Mitochondrial Eve has a new great-granddaughter!

Back in 2018, Goran Runfeldt and Bennett Greenspan at FamilyTreeDNA noticed something unusual about a few mitochondrial DNA sequences, but there weren’t enough sequences to be able to draw any conclusions. As time went on, more sequences became available, both in the FamilyTreeDNA database and in the academic community, including an ancient sequence.

This group of sequences did not fit cleanly into the phylogenetic tree as structured and seemed to cluster together, but more research and analysis were needed.

Were these unique sequences a separate branch? One branch or several? What would creating that branch do to the rest of the tree?

Given that Phylotree, last updated in 2016, did not contain an applicable branch, what were we to do with these puzzle pieces that really didn’t fit?

These discussions, and others similar, led to the decision to launch the Million Mito Project to update the mitochondrial phylogenetic tree which is now 6 years old and seriously out-of-date. For the record, phylogenetics on this scale is EXTREMELY challenging, which is probably why Phylotree hasn’t been updated, but that’s a topic for another article, another day. Today is the day to celebrate haplogroup L7.

Haplogroup L7

The Million Mito team knew there were lots of candidate haplogroups waiting to be formed near the ends of the branches of the phylotree, but what we didn’t expect was a new haplogroup near the root of the tree.

Put another way, in terms that genealogists are used to, the new branch is Eve’s great-granddaughter.

Haplogroup L now has 8 branches, instead of 7, beginning with L0. We named this new branch haplogroup L7 in order not to disrupt the naming patterns in the existing tree.

Let’s take a look.

I used the phylogenetic tree from our paper and added Eve.

Just to be clear, we aren’t talking literal daughters and granddaughters. These are phylogenetic daughters which represent many generations between each (known) branch. Of course, we can only measure the branches that survived and are tested today or are found in ancient DNA.

The only way we have of discovering and deciphering Eve and her “tree” of descendants is identifying mutations that occurred, providing breadcrumbs back in time that allow us to reconstruct Eve’s mitochondrial DNA sequence.

Those mutations are then carried forever in daughter branches (barring a back-mutation). This means that, yes, you and I have all of those mutations today – in addition to several more that define our individual branches.

You can see that Eve has two daughter branches. One branch, at left, is L0.

Eve’s daughter to the right, which I’ve labeled, is the path to the new L7 branch.

Before this new branch was identified, haplogroup L5 existed. Now, Eve has a new great-granddaughter branch L5’7 that then splits into two branches; L5 and L7.

L5 is the existing branch, but L7 is the new branch that includes a few sequences formerly misattributed to L5.

Even more exciting, the newly discovered haplogroup L7 has sub-branches too, including L7a, L7a1, L7b1 and L7b2.

In fact, haplogroup L7 has a total of 13 sublineages.

How Cool is This?!!

Haplogroup L7 is 100,000 years old. This is the oldest lineage since haplogroup L5 was discovered 20 years ago. To put this in perspective, that’s about the same time the first full sequence mitochondrial DNA test was offered to genealogists.

It took 20 years for enough people to test, and two eagle-eyed scientists to notice something unusual.

Hundreds of thousands of people have had their mitochondrial DNA tested, and so far, only 19 people are assigned to haplogroup L7 or a subgroup.

One of those people, shown as L7a* on the tree above, is 80,000 years removed from their closest relative. Yes, their DNA is hens-teeth rare. No, they don’t have any matches at FamilyTreeDNA, just in case you were wondering😊

However, in time, as more people test, they may well have matches. This is exactly why I encourage everyone to take a mitochondrial DNA test. If someone is discouraged from testing, you never know who they might have matched – or how rare their DNA may be. If they don’t test, that opportunity is lost forever – to them, to other people waiting for a match, and to science.

Are there other people out there with this haplogroup, in either Africa or the diaspora? Let’s hope so!

With so few L7 people existing today, it looks like this lineage might have been on the verge of extinction at some point, but somehow survived and is now found in a few places around the world.

Ancient DNA

One 16,000-year-old ancient DNA sample from Malawi has been reclassified from L5 to L7.

This figure from the paper shows the distribution of haplogroup L within Africa, and the figure below shows the Haplogroup L7 range within Africa, with Tanzania having the highest frequency. Malawi abuts Tanzania on the Southwest corner.

Where in the World?

Checking on the public tree at FamilyTreeDNA, you can see the new L5’7 branch with L7 and sub-haplogroups beneath.

We find L7 haplogroups in present-day testers from:

  • South Africa
  • Kenya
  • Ethiopia
  • Sudan
  • United Arab Emirates
  • Yemen
  • Tanzania

It’s also found in people who live in two European countries now, but with their roots reaching back into Africa. Surprisingly, no known African-Americans have yet tested with this haplogroup. I suspect finding the haplogroup in the Americas is just a matter of time, and testing.

The FamilyTreeDNA customers who are lucky enough to be in haplogroup L7 have had their haplogroup badges updated.

If you are haplogroup L at FamilyTreeDNA, check and see if you have a new badge.

Credit Where Credit is Due

I want to give a big shout-out to my colleagues and co-authors. Dr. Paul Maier (lead author,) Dr. Miguel Vilar and Goran Runfeldt.

I can’t even begin to express the amount of heavy lifting these fine scientists did on the long journey from initial discovery to publication. This includes months of analysis, writing the paper, creating the graphics, and recording a video which will be available soon.

I’m especially grateful to people like you who test their DNA, and academic researchers who continue to sequence mitochondrial DNA in both contemporary and ancient samples. Without testers, there would be no scientific discoveries, nor genealogy matching. If you haven’t yet tested, you can order (or upgrade) a mitochondrial DNA test here.

I also want to thank both Bennett Greenspan, Founder, and President, Emeritus of FamilyTreeDNA who initially greenlit the Million Mito Project in early 2020, and Dr. Lior Rauschberger, CEO who continues to support this research.

FamilyTreeDNA paid the open access fees so the paper is free for everyone, here, and not behind a paywall. If you’re downloading the pdf, be sure to download the supplements too. Lots of graphics and images that enhance the article greatly.

Congratulations to Mitochondrial Eve for this new branch in her family tree. Of course, her family tree is your family and mine – the family of man and womankind!

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Ancestry Only Shows Shared Matches of 20 cM and Greater – What That Means & Why It Matters

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Recently, I’ve noticed an uptick in confused people who’ve taken Ancestry’s DNA test.

They are using shared matches, which is a great tool and exactly what they should be doing, but they become confused when no shared matches appear with some specific people.

This is especially perplexing when they know through information sharing or because they manage multiple DNA kits that those two people who both match them actually do share DNA and match each other, meaning they “should” appear on a shared match list. Or worse, yet, conflicting match information is displayed, with one person showing the shared match, but the other person reciprocally does not.

What gives?

That’s exactly what this article addresses. It’s not quite as simple as it sounds, but it’s certainly easier once you understand.

What Are Matches and Shared Matches?

Matches occur when two people match each other. From your perspective as a DNA tester, matches are people who have taken DNA tests and appear on your match list because you share some level of DNA equal to or greater than the match threshold of the vendor in question.

At Ancestry, that minimum matching threshold is 8 cM (centimorgans) of matching DNA.

Individual matches are always one-to-one. Your match list is a list of people who all match you.

So, you match person 1, and you match person 2, individually.

Your matches may or may not also match each other. If they do match each other in addition to matching you, that’s a shared match which is a hint as to a potential common ancestor between all three people.

Shared matches are a list of people who match you PLUS any one other match on your list. In other words, shared matches are three-way matches.

In the diagram above, you can see that you match Match 1 and you also match Match 2. In this case, Match 1 and Match 2 also match each other, so all three of you match each other, but not necessarily on the same segment. Therefore, you’re all three shared matches, as shown in the center of the three circles.

Viewing Shared Matches

To view a list of people who match you and Match 1, you would request shared matches with Match 1 by clicking on “View Match” or “Learn More” on your match list, then on “Shared Matches” on the next screen.

The resulting shared match list consist of people who match you AND Match 1, both. It’s easy to make assumptions about why you have shared matches, but don’t.

Shared Matches are Hints

A shared match CAN mean:

  • That all three people share a common ancestral line.
  • You share a common ancestor with Match 1 and Match 2, but Match 1 and 2 match each other because they share an entirely different ancestor.
  • You match Match 1 because you share DNA from Ancestor A and you match Match 2 because you share DNA from Ancestor B. Match 1 and 2 match each other either because they share one or both of those common ancestors.
  • Match 1 and Match 2 might match because Match 1 and Match 2 share an ancestor that isn’t related to you.
  • That one (or more) of the matches is identical by chance, meaning the DNA combined from two parents in a random way that just happens to match with someone else.

Shared matches are great hints to be sifted for relevance. The operative word here is hint.

What If We Don’t Have Shared Matches?

Conversely, NOT having a shared match doesn’t mean you don’t share a common ancestor.

Sorry about the triple negative. Let me say that another way, because this is important.

Even though you and someone else aren’t on a shared match list, you might still share DNA and you may share a common ancestor, whether you share their DNA or not.

Ancestry’s shared matches work differently than shared matches at other vendors. Before we discuss that, let’s talk about why shared matches are important.

Why Do Shared Matches Matter Anyway?

Matches and shared matches are how genealogists perform two critically important functions:

  • Verifying “known” ancestors. Sometimes paper trails aren’t accurate and certainly, neither are trees.
  • Identifying unknown ancestors. Looking for common families among shared DNA matches is a HUGE hint when tracking down those pesky unknown ancestors.

I wrote about shared matches, here, when Ancestry purged segments under 8 cM, but I think the message about the limitations of shared matches and how the process actually works deserves its own article, especially for new users. Shared matches and segment cM numbers can be quite confusing, but they don’t need to be.

I wrote an article titled DNA Beginnings: Matching at Ancestry and What It Means that includes lots of useful information.

Ok, now let’s look specifically at using shared matches and why sometimes shared matches just don’t seem to make sense.

Matches

By far, the majority of your matches at any vendor will be more distant matches. That’s because you have thousands of distant relatives, most of whom you don’t know (yet).

You’ll only have a few closer relatives.

At Ancestry, I have 102,000+ total matches, of which more than 97,000 are distant matches. Based on these numbers, keep in mind that about 95.74% of my matches are distant, meaning 20 cM or below, and yours probably are too. You’ll need that number later.

Note that 20 cM is Ancestry’s threshold between close matches and distant matches.

That’s about exactly where you’d expect, on average, to see a 20 cM match – generally at or further back than 4th cousins. 20 cM is roughly the 4th to 6th cousin level.

Of course, you won’t match most of your 5th cousins at all, yet you’ll match some with more than 20 cM. That’s just the roll of the genetic dice.

Closer ancestors (meaning closer matches) is also the area of genealogy where much of the lower-hanging fruit has been plucked.

In my case, the closest unknown ancestor in my tree occurs at the 6th generation level and I have 5 or 6 missing sixth-generation ancestors – all females with no surnames. Two have no names at all.

Click to enlarge any image

How Much DNA Do Cousins Share?

One of my priorities as a genealogist is to identify those unknown people, which is why matches, and shared matching at that level are critical for me.

Ancestry tells me that this 20 cM match is likely my 4th-6th cousin.

At DNAPainter, in the Shared cM Tool, you can enter the total cM number of a match, which is the total amount of DNA that you share after Ancestry’s Timber algorithm has been applied. The range of relationship probabilities for 20 cM is shown below.

For a total match of 20 cM with another individual, several relationships ranging between half 3C2R/3C3R and 8th cousins are the most probable relationships at 58%.

For the record, this is total cM, which does not necessarily mean one segment. Ancestry reports the number of segments, but Ancestry does not show you the segment locations, nor do they have a chromosome browser. Without a chromosome browser, you have no way of determining whether or not you match with shared matches on the same segment(s). In other words, there is no triangulation at Ancestry, meaning confirmation of a specific shared DNA segment descended from a common ancestor. You can find triangulation resources, here.

Close Matches

The best way to figure out how you are related to closer matches (assuming you don’t already know them and Ancestry has not found a common ancestor) is using shared matches. Hopefully, you will share matches with people you do know or with whom you’ve already identified your common ancestor.

One of my relatively close DNA matches at Ancestry is Lonnie. I don’t know Lonnie, but it looks like I should because he’s probably a 1st or 2nd cousin. We share 357 cM of DNA over 20 segments.

I thought I knew all of my 1st and 2nd cousins. Let’s see if I can figure out how I’m related to Lonnie.

By clicking on Lonnie’s name on my match list, then on Shared Matches, I can determine that Lonnie and I connect through my Estes and Vannoy lines based on who we both match, which means that our common ancestor is either my paternal grandfather or my great-grandparents, Lazarus Estes and Elizabeth Vannoy.

You can see the notes I’ve made about these matches I share with Lonnie.

Viewing Lonnie’s unlinked tree verifies the ancestral line that shared matches suggest. An unlinked tree means that Lonnie has not linked his DNA test to himself in his tree. Since Ancestry doesn’t know who he is in the tree, they can’t find a common ancestor for me and Lonnie. However, I can by viewing his tree.

Our common ancestor is Lazarus Estes and his wife, Elizabeth Vannoy. Therefore, Lonnie is my 2nd cousin.

That wasn’t difficult, in part because I had already worked on the genealogy of our common matches and Lonnie had a small unlinked tree where I could confirm our common ancestor.

Now let’s move to more distant, not-so-easy matches.

Distant Matches

I’ve spent a lot of time over the years identifying common ancestors with my matches.

When I make that connection, whether or not Ancestry has been able to identify our common ancestor, I make notes about common ancestors and anything else that seems relevant. Notes very conveniently show on my match list so I don’t need to open each match to see how we are related.

Ancestry does identify potential common ancestors using ThruLines. Note the word potential. Ancestry compares the trees of you and your matches searching for common ancestors and suggests connections. It’s up to you to verify. ThruLines are hints, not gospel. Additionally, you may have multiple ancestral links to your matches. Ancestry can only work with the fact that you have a DNA match with someone AND the user-provided trees of your matches.

Ancestry’s ThruLines only reach back a maximum of 7 generations to suggest common ancestors. At 7 generations distance, you’d be a 5th cousin to a descendant who is also 7 generations downstream from that ancestor.

The information from DNAPainter, who utilizes the Shared CM Project compiled data shows that the most likely amount of shared DNA for 5th cousins, is, you’ve guessed it – 20 cM.

Jacob Dobkins is my 7th generation ancestor. I have ThruLines for him and his wife, but not for their parents who are one generation too distant for ThruLines. I’d LOVE to see Ancestry extend ThruLines another 2 or 3 generations.

ThruLines matches me with people who descend from Jacob through his other children. Other children are important because the only ancestors you share with those people are (presumably) that ancestral couple.

Matches with Jacob’s descendants range from 8 cM (the smallest amount Ancestry reports) to 32 cM.

Here’s an example.

Ancestry displays some shared matches with all of your matches, regardless of the size of your match to that person. However, Ancestry ONLY shows shared matches to a third person if you share more than 20 cM of DNA with that third person.

For example, I match KO with 8 cM of DNA. Ancestry shows my shared matches with KO, below.

I only have 3 shared matches with KO. I only match KO at 8 cM, but I match our shared matches at 39, 31 and 21 cM, respectively.

Ancestry does NOT show shared matches below 20 cM, so it’s unknown how many additional shared matches KO and I actually have if shared matches less than 20 cM were displayed.

Perspective is Critical

Whether you see a shared match or not is sometimes a matter of perspective, meaning which of two people you request shared matches with.

In this case, I requested shared matches with KO. I only share 8 cM of DNA with KO, but that doesn’t matter. The amount of DNA you share with the person you’re requesting shared matches with is irrelevant.

Ancestry’s Shared Matches with KO include Ker

I will see shared matches with KO to anyone we mutually share as matches above 20 cM, including Ker.

If I request shared matches with Ker, with whom I share 39 cM of DNA, I will see all of our mutual matches at 20 cM (or greater) of DNA. However, that does NOT include KO because I only share 8 cM of DNA with KO.

This restriction applies regardless of how much DNA KO and Ker share, which is an unknown to me of course.

Ancestry’s Shared Matches with Ker does NOT include KO

Nothing has changed between these matches, yet KO does not appear on my shared matches list with Ker when I request shared matches with Ker.

I still share 8 cM with KO and 39 cM with Ker. KO and Ker still both match each other. The only difference is that Ker shows up on my shared match list with KO because I share more than 20 cM with Ker. However, when I request a match list with Ker, KO does NOT appear because I only share 8 cM with KO.

This is the source of the confusion and often, why people disagree about shared matches. It’s kind of a “now you see it, now you don’t” situation.

If a person shows as a shared match depends on:

  1. Whether the third person actually does share DNA with the tester and the person they’ve asked for shared matches with
  2. Whether the third person shares 20 cM DNA or more with the tester, the person requesting the shared match list with one of their matches

Whether someone appears on a shared match list can literally be a matter of perspective unless the match and the shared matches all match the tester at 20 cM or larger.

Another Example

Let’s look at a larger match to a descendant of the same ancestor.

I share exactly 20 cM with Joyce, my 5C1R.

Viewing my shared matches with Joyce, I match 50 other people that she matches as well.

I only share 25 cM of DNA with the smallest match with Joyce. Apparently, there are no matches with Joyce with whom I share between 20 and 25 cM of DNA.

Bottom Line

Here’s the bottom line.

Ancestry NEVER shows any shared matches below 20 cM from the perspective of the tester, meaning people who match you and someone else, both.

If you recall our earlier math, that means that approximately 95.74% of my shared matches aren’t shown.

This puts shared matches in a different perspective because now I realize just how many matches I’m not seeing.

Why is This Confusing?

If you aren’t aware of this shared match limitation, and that a majority of your shared matches are actually below 20 cM, you may interpret shared match results to mean you actually DON’T share specific matches with that other person. That isn’t necessarily true, as we saw above with KO and Ker.

Furthermore, let’s say you manage your DNA kit plus 3 more, A, B and C. Because you manage all 4 kits, that means you can see the results for all 4 people.

  • A – 10 cM
  • B – 20 cM
  • C – 40 cM

From the perspective of YOUR kit, you will see some shared matches FOR all of those matches.

What you won’t see is shared matches if you don’t match the shared match (third person) at 20 cM or greater.

Always remember, shared match information at Ancestry is ALWAYS from the perspective of your DNA kit combined with the person with whom you request the match.

I’ve put this information in a grid because that’s how I make sense of things like this.

Here are your matches. When you click on shared matches with person A who you match at 10 cM, you’ll see both person B and person C as shared matches since you match both of those people at 20 cM or larger. You WILL see 20 cM shared matches, but you will not see 19 cM shared matches.

When you request shared matches for A, you will see both B and C.

When you request shared matches with kits B and C, you will not see A because you only match them at 10 cM.

However, from the perspective of DNA kits A, B and C, shared matches look different.

Let’s look at shared matches from the perspective of Kits A, B and C.

Kit A matches you, Kit B and C, but can only see Kit B as a shared match because matches with you and Kit C are under 20 cM.

Kit B doesn’t match C at all, so they clearly won’t have shared matches. However, they do match you and Kit A, both at 20 cM and over, so Kit B will see you as a shared match with Kit A, and Kit A as a shared match with you.

Kit C doesn’t match Kit B, so no shared matches with that person at all. Kit C does match you and Kit A. However, when Kit C clicks on shared matches for you, Kit A doesn’t show up because they only match Kit A on 9 cM. When Kit C clicks on Kit A for shared matches, you ARE listed as a shared match because you share 40 cM of DNA with Kit C.

There’s no way to discern whether two of your matches match each other unless they show as a match in the shared match tool. You can’t tell if their absence on the shared match list means they actually don’t match, or their shared match absence is because they match you at less than 20 cM.

Whew, that was a mouthful.

You may need to refer back to this from time to time if you’re confused by your shared matches at Ancestry.

If you need to remember rules, remember this.

  1. You can obtain shared matches with yourself plus any match, regardless of how much or how little DNA you share with that one match. Prove this to yourself by finding a match under 20 cM, like my 8 cM match, and viewing your shared matches.
  2. No one will show on a shared match list with another person unless they match you at 20 cM or greater. Prove this to yourself by viewing the smallest shared match with anyone.

Strategy

The takeaway of this is if you have a larger (20 cM or over) and smaller match (under 20 cM), always request shared matches from the perspective of the smaller match because the smaller match won’t show up as a shared match on any shared match list.

The only way you can see shared matches that includes people under 20 cM is to request to view shared matches with individual people who match you below 20 cM. 

In my case, I will never see KO on any shared match list because I only match KO at 8 cM. However, I can request my shared matches with KO in which case I’ll see all 20 cM or greater shared matches with KO.

Alternatives

Every vendor provides a shared match feature, and each functions differently.

In the chart below, I’ve provided basic shared match information for each vendor.

If you’re interested in uploading your DNA file from Ancestry or another vendor, I’ve provided upload/download step-by-step instructions for each vendor, here.

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Million Mito Project Team – Introduction and Progress Update

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Let me introduce you to the Million Mito Project team.

Left to right, Goran Runfeldt, Dr. Paul Maier, me, and Dr. Miguel Vilar. And yes, I know we look kind of like a band😊. The Merry Mito Band maybe, except, trust me, I can’t sing.

Yes, we finally, finally got to meet in person recently, and let me tell you, that was one joyful meeting. I hadn’t realized that while I know everyone, not everyone else had met in person before.

We have been working for almost two years together via Zoom, but separately. Just 10 days after the Million Mito Project was announced, we went into Covid lockdown.

It’s difficult to work remotely on such a huge collaborative project, but we have been making inroads, albeit slower than we had initially hoped.

Complicating this was the merger of FamilyTreeDNA with myDNA in January of 2021, with Bennett Greenspan stepping down as the CEO in that process. Bennett greenlit the Million Mito project initially. (Thank you, Bennett!)

Thankfully, the new CEO, Dr. Lior Rauschberger continued that greenlight without hesitation as soon our team was able to inform him about this wonderful scientific project that was underway. (Thank you, Lior!)

I can’t tell you what a HUGE relief that was.

While all change is challenging, and complicated by the Covid landscape, life events, and geographic distance, that merger really was the right decision. Lior is committed to scientific research, discovery, and the genealogy marketspace. He’s looking to expand, not contract.

You’re probably wondering where we are now in the Million Mito process.

Million Mito Project Update

I’d like to provide a brief update.

  • We have an academic paper in the final stages of the submission process, but this paper is not the final tree. It is, however, something extremely cool and important to the history of womankind! I can’t say more until publication, but I’ll write an article when the paper is published.
  • The team hopes to work with a million samples between all sources including FamilyTreeDNA testers, research-consented Genographic samples, Genbank, and other academic samples. Not all samples from those sources are full mitochondrial sequences, or necessarily pass our QC checks.

If you haven’t yet taken a full sequence test, you can help reach the one million goal by ordering a mitochondrial DNA test at FamilyTreeDNA, here. If you tested at a lower level some years back, please sign on to your account and upgrade so you can be a part of this scientific frontier.

  • We discovered that the authors of Phylotree never documented the “recipe” for reconstructing the tree behind the scenes, so we can’t exactly use the recipe for Phylotree as the basis for constructing a future tree.
  • We have been in the process of writing phylogenetic software that arrives at a similar tree to use as a baseline reference structure in order to preserve as many of the current Phylotree haplogroup names as possible.

Hand curation and placement is possible for hundreds or a few thousand samples, but it’s not possible for large numbers. While phylogenetic software to do this kind of work has existed for a long time, it typically can’t handle huge trees like what we are building.

Phylogenetic methods also struggle with highly recurrent mutations, and rapid star-burst expansions that we see on the human trees. A phylogenetic problem of this magnitude requires lots of innovations to correctly interpret lineage history from complex mutations.

Automated software to handle very large numbers of sequences must be adapted or developed.

  • Furthermore, simply building upon an existing scaffold without automating the process does not provide an ongoing, sustainable procedure to discover where new dividing branches are discovered internally within the tree, versus at the tips. In other words, adding new branches based on common mutations is only easy when you’re simply appending a new haplogroup to an existing one.

For example, I might have a new haplogroup J1c2f1 derived from J1c2f. That’s easy. It’s another matter entirely if haplogroup J1 itself, high up in the tree, were broken into multiple new branches. Only automated software can “reconstruct” the tree regularly to discover new major branches as the results of more testers become available.

Challenges

Let me share some examples of the kinds of challenges that we’ve encountered. Not only are these interesting, but they are also educational.

These figures are from Paul Maier’s RootsTech presentation, which I strongly recommend that you view, here.

Mitochondrial DNA is both fascinating and habit-forming. The more you know, the more you want to know.

Let’s start with the basics. Haplogroups are defined by one or more mutations that everyone upstream does NOT have, and everyone downstream DOES have.

Pretty simple so far, right!

Haplogroup-Defining Mutations

Here’s an example of a nice simple mutation that is one of the multiple mutations that define haplogroup L1, near the base of the mitochondrial tree (Mitochondrial Eve) in the center. At location 3666, the “normal” value is G, but in this branch, the G in that position has been replaced by an A.

You can see that the other haplogroups shown in the circle by black dots don’t have the G-to-A mutation at location 3666, but the red dot locations do carry that mutation. Therefore, G3666A is one of the mutations that defines haplogroup L1. Haplogroups can be defined by only one unique mutation, or multiple mutations.

Multiple Haplogroup-Defining Mutations

Haplogroups with multiple mutations that define that specific haplogroup are candidates to be split into multiple branches forming new haplogroups at some point in the future when other people test who have:

  1. One or the other of those mutations if there are only two
  2. A subset of the mutations
  3. But not all of the mutations

Click on images to enlarge

For example, in the view of the public mitochondrial haplotree at FamilyTreeDNA which you can view here, you see that haplogroup L1 is defined by a total of 6 mutations. Someday, people may test that only have half (or a portion) of those mutations which would cause haplogroup L1 to split or branch into two separate haplogroups.

Unstable Mutations

Some mitochondrial locations are unstable, such as 16519C, along with a few other hypervariable locations. By unstable, I mean that they have mutated back and forth in the tree many times. The historical branching patterns of such unstable mutations can be difficult to decipher (the technical term is “saturation”), suggesting perhaps that they should not be the foundation for a new haplogroup.

Do we ignore those unstable locations entirely?

After discounting those well-known unstable locations, we still find some mutations, often in the HVR (hypervariable) regions that occur close to 100 times in the full tree.

This mutation at location 150 from C to T occurred four distinct times just in this small subset of haplogroup L. You can see the 4 locations I’ve bracketed with red boxes.

Is C150T stable enough to form a haplogroup? Multiple haplogroups? Should it be used high in the tree if this affects the complete downstream structure?

This same mutation occurs additional times further downstream in the tree, as well.

Reverse Mutations

Of course, some haplogroups are defined by reverse mutations, where the original mutation reverts back to its original state.

What about locations that have as many as 3 reverse mutations, which means that one location mutates back and forth 6 times in total? Kind of like a drunken sailor zigging and zagging along the street.

If we counted each mutation and reversal as a new haplogroup, we would have 6 new haplogroups based on this one single location in one parent haplogroup. Is that accurate, or should we ignore it altogether?

Here’s an example of one mutation and a corresponding back mutation.

In this scenario, the mutation of location 7055 from A to G occurred once in the formation of haplogroup L1. However, a back mutation took place, signified by the ! (exclamation mark) after the A, which is a defining mutation for haplogroup L1c3. All of the other L1c haplogroups still carry the A to G mutation, while L1c3 does not.

In some scenarios, the same location bounces back and forth. Should it still be counted as a haplogroup defining mutation, or is it simply “noise”?

Heteroplasmies

How do heteroplasmies play into this scenario?

Heteroplasmies occur when more than one value is discerned in an individual’s DNA at a specific location. Heteroplasmies do not define haplogroups, but they are reported in your personal results.

To be reported as a heteroplasmy, both values need to be detected at a level of over 20%. In the above scenario, if both G and A were found greater than 20% of the time, it would be counted at a heteroplasmy with a special notation.

For example, if G and A are both found more than 20% of the time, the notation would be R instead of either G or A. If the location was G7055, above, and G and A were both found above 20%, the notation would be G7055R.

However, if G was found 81% of the time or more, then it would be counted as G, which is “normal,” and if A was found 81% of the time or more, then the value would be reported as A, a mutation. If we see the normal state of G, then an A, then a G, is that a mutation and a back mutation? How many samples would need to contain that back mutation to count it as a mutation and not an aberration, an undetected borderline heteroplasmy slipping back and forth over the threshold, or simply noise?

Transitions Versus Transversions

There are two types of mutations, transitions and transversions, that probably should be weighted differently – but how differently, and why?

Some types of mutations occur more easily than others and are therefore more common. Paul explains this very well in his RootsTech video, but in a nutshell, transitions between T/C and A/G are much more common than transversions between A/C, G/T, C/G, and A/T. Therefore, transversions are noted with a small letter, shown above as T7624a.

In phylogenetics, the rarer mutation which is chemically less likely to occur (transversion) is weighted more heavily than the likelier mutations (transitions).

Insertions

Insertions are another type of challenge. Insertions happen when extra DNA is inserted at a specific location, kind of like the genetic equivalent of cutting in line.

In this graphic, we see that at location 5899, there’s an extension of .XC, written as 5899.XC. This means that at this location, you’ll find an unknown or varying number of additional Cs inserted. Paul showed several example sequences in the box at upper left. In some people who have this mutation, there are only one or two inserted Cs. In other people, there are several Cs, shown in the bottom two sequences.

You might recognize this as a phenomenon similar to Y DNA STRs which are short tandem repeats. Of course, we don’t use STRs for haplogroup identification in Y DNA. How should we handle insertions, especially multiple insertions, in building the Mitotree?

Deletions

We see deletions of DNA too, indicated by a small “d” after the location. In some cases, we find large deletions.

At location 8281, there is a 9 base-pair deletion (8281 through 8289) that is one of the haplogroup defining mutations for haplogroup L0a2. We find a 9 base-pair deletion in exactly the same location again within subclades of haplogroups B and U.

Is there something about this specific location that makes it more prone to deletions, and specifically a deletion of exactly 9 base pairs?

Seeking Answers

Of course, we’re seeking all of these answers.

The team has been writing code to create structural trees based on various scenarios and trying to determine which ones make the most sense, all factors considered.

The current official tree, meaning the 2016 Build 17 version of Phylotree, is based on about 8,000 samples. Working with one million versus 8,000 is a challenge that ramps exponentially, necessitating substantial computing power.

Working with 125 times more data provides amazing potential, but it has also introduced challenges that never had to be addressed before. It’s evident, to us at least, why Phylotree wasn’t updated after 2016. The tools simply don’t exist.

Sneak Peek

We fully expect hundreds if not thousands of new haplogroups to form. Today, Paul’s haplogroup is U5a2b2a which was formed about 5,000 years ago during the Bronze Age.

The haplogroup itself is useful to determine roughly where your ancestors were at that time, and often provide information about more recent population group history, but you need mitochondrial DNA matching to provide more genealogically useful information.

Paul’s test results show that he has 8 extra mutations, which means those mutations are in addition to his haplogroup-defining mutations. These extra mutations are what make genealogical matching so useful.

Paul has 16 full sequence matches that match him at a genetic distance of 3 mutations or less, although due to privacy restrictions at FamilyTreeDNA, we can’t see which matches share which mutations.

Given that Paul has 8 extra mutations, this means that it’s possible that one or more new haplogroups will be formed using some or all of those 8 extra mutations, and that those people who match him at a GD of 3 or less will very likely be members of a newly formed haplogroup.

Here’s a comparison of Paul’s haplogroup today, at left, with the newly created U5a2b2a branch and resulting subclades in a beta version of our experimental Mitotree, at right. This moves Paul’s new haplogroup, the pink node at right, from 5,000 to 500 years ago which is clearly within a genealogically relevant timeframe.

The single haplogroup, U5a2b2a, now has been expanded to 7 subgroups. If U5a2b2a is representative of the expansion capability of the entire tree, that’s a 7-fold increase.

Of Paul’s 16 matches, those with the same new haplogroup are those where he needs to focus his genealogical research.

Where Are We?

This is not a commitment, but we expect to release a sneak preview of the new Mitotree this year.

If you have extra or missing mutations, especially in the coding region, you and your close matches may very well receive a new, expanded haplogroup.

Highly refined haplogroups will improve the ability to use mitochondrial DNA for genealogical purposes – similar to what the Big Y-700 SNP testing and the expanded haplotree have done for Y DNA.

Like with Y DNA, you’ll want to use your new haplogroup in combination with genealogical trees.

The more people that test, the more success stories emerge, and the more people that WILL test. Just think what would happen if everyone who took a Y or autosomal DNA test also took a mitochondrial DNA test. We’d be bulldozing through brick walls every day.

I don’t know about you, but I have so many women in my trees with no parents. I need more tools and can hardly wait.

Resources

The new Mitotree is fueled by the Million Mito Project which is fueled by full sequence DNA testing, so please purchase yours today.

And yes, in case you were wondering, the new Mitotree will be free and public, just like the existing Mitochondrial DNA Tree and Y DNA Tree are at FamilyTreeDNA today.

You can read more about the Million Mito project here and here.

You can watch Paul’s Million Mito RootsTech presentation, here.

Paul, Miguel and I will be co-presenting Mitochondrial DNA Academy on Saturday, April 23, during the ECCGC Conference which you can read about here and register here.

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Hurry: Relatives at RootsTech Ends March 25 – Search for Y & Mitochondrial DNA Cousins While You Can

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Relatives for RootsTech is still available through March 25th, even though RootsTech, the event, is over for this year. (Obviously, the video sessions are still available.)

Relatives at RootsTech provides participants with the opportunity to see cousins, organized in different ways, including by ancestor, with a path for both of you drawn back to your common ancestors.

Be sure to fully utilize the Relatives at RootsTech connections to easily find cousins who descend appropriately to be testing candidates for Y DNA and mitochondrial DNA for your ancestors. I’ve included step-by-step instructions in this article along with a few hints I’ve discovered.

Just navigate to RootsTech, here, and scroll down to the relatives at RootsTech button.

Click that button, then on “view relatives” and voila, here you are.

FamilySearch has made this easy by displaying your relatives by ancestor, at least for several generations back in time. You can see how many of your cousins descend from any particular ancestor.

While my closest ancestors are showing few cousins, more distant ancestors further down my relatives list, (and further back in my tree,) have hundreds.

It’s Easy Peasy

Eventually, every single line brick walls. Y DNA and mitochondrial DNA are the ONLY types of DNA you can use that doesn’t divide in every generation and remains as reliable 10 or more generations ago as today. Y DNA and mitochondrial DNA are laser lights shining back through time. We need them for every single ancestral line to push beyond that brick wall, whenever and wherever we hit it.

I’ve spent time in the past few days fishing for cousins and messaging people who are good candidates to represent lines that I don’t have represented in my DNA pedigree chart.

In my own desktop software, I enter my ancestor’s haplogroup as a middle name. The * means I’ve written a 52 Ancestors series article about this person. (I don’t do this in public trees, just my own.)

I can see at a glance which ancestors don’t have haplogroups, which means I need to find cousins who descend appropriately to have inherited either the Y DNA or the mitochondrial DNA of that ancestor.

The blue boxes above represent the Y DNA inheritance path, and the red, mitochondrial inheritance. You can read more about Y and mitochondrial DNA inheritance paths, here.

Neither Y nor mitochondrial DNA are admixed with the DNA of the other parent, so it’s a rich source of information that never divides during meiosis. This gives us the ability to see far back in time without dilution.

Focus

I created a small spreadsheet so I wouldn’t lose track of whose DNA I’m looking for and the message I sent to various cousins.

By focusing only on ancestral lines I specifically need, I’ve eliminated a lot of busy work. Initially, I was going to record every cousin, but there are too many for me to be able to complete that task. Now I’m focused on:

  • Lines where I have very few matches. These may represent closer cousins I haven’t yet met, or people in the Netherlands who are now participating. I found a new Dutch cousin. Hopefully they will reply to my message.
  • Y DNA lines
  • Mitochondrial DNA lines

Timesaving Hint

When searching in this manner, find your most distant ancestor on the relatives list in that line. For example, I only have two cousins on my Lazarus Estes list, but as I look at ancestors on up that Estes line, I have several more by the time you get to Moses Estes, 4 generations earlier. My two cousins who descend from Lazarus will ALSO be on the Moses Estes list – as will all the rest of my cousins who descend from Estes males between Lazarus Estes and Moses Estes.

Moving to the earliest ancestors in a line immediately saves you a heap of time because you don’t need to view your cousins in the closer generations.

Y DNA

Finding appropriate cousins for Y DNA is easy. They will generally carry the surname of the ancestor in question. If I’m searching for a descendant of Andrew McKee (c1766-1814), I’ll just look for McKee surname cousins on my list.

To see how your cousin descends from your common ancestor click on Relationship. A nice dual path is shown to your common ancestors.

I found a female, so I messaged her and ask if she has a father or brother or uncle who would be willing to test to represent the McKee Y DNA line.

In my message, I briefly explain how beneficial this would be for everyone in that line and might well help break down those upstream brick walls. Who were Andrew’s parents?

I don’t know now, but I’d surely know more after a Y DNA test. So would she!

In this next example, my cousin is male, and the last male shown descending from Andrew is Robert Clayton McKee. I “presume” my cousin descends through two upstream males, but sometimes that’s not the case. Either of those two greyed out people could be females. I’m always “gentle” in these messages and say that “It appears that you descend from Andrew through all males. FamilySearch conceals the identity of your closest generations for privacy.”

I ask my cousin to confirm how they descend and ask if they have tested or are interested in DNA testing. I also provide my email address and offer a testing scholarship.

Mitochondrial DNA

Locating mitochondrial DNA testing candidates takes slightly more effort, but can be VERY productive.

Let’s say I’m searching for a mitochondrial DNA candidate for Andrew McKee’s wife.

Notice, I said “wife” and did not mention her name. All we really know, from a deed signature releasing her dower right, is that her first name is Elizabeth. The reason I would be seeking her mitochondrial DNA is to figure out who her parents were.

At FamilySearch, Elizabeth has been assigned a full name, including surname, but there are no sources that provide her surname.

DO NOT DISREGARD THIS RECORD!

My first inclination is to disregard this record because there is no evidence that Barnes is Elizabeth’s surname, at least not that I’ve ever seen. If any reader has actual evidence, please do share.

However, in this case, we are searching for anyone descended from the wife of Andrew McKee, REGARDLESS OF HER NAME. Her name, in this context and for this purpose does not matter.

In other words, if we can find a candidate for Andrew’s wife’s mitochondrial DNA, we may then be able to determine if indeed she does match someone in the Barnes family line.

It’s very easy to skim your matches ancestral line. If you see any blue in their lineage, indicating a male in your cousin’s line, that’s an immediate “no,” so you can just proceed to the next cousin in your list.

Mitochondrial DNA is only passed from women to their children. Men don’t pass it on, so a male in that line is a blocker. Andrew McKee Jones, in this example, inherited his mother’s mitochondrial DNA, but his children inherited the mitochondrial DNA of their mother.

Fortunately, FamilySearch also identifies daughter or son when names are ambiguous.

Scholarships

I always offer a DNA testing scholarship at FamilyTreeDNA for the appropriate Y DNA or mitochondrial test. FamilyTreeDNA also offers their autosomal Family Finder test, of course, and I often include that test in the scholarship.

Other vendors do not offer Y and mitochondrial DNA testing. However, if your cousins have already tested autosomally at Ancestry, 23andMe, or MyHeritage, they can upload their DNA files to FamilyTreeDNA for free after you order their scholarship test. Step-by-step upload instructions can be found, here.

I always check to see if Y DNA and mtDNA testers’ matches are also autosomal matches. That too can provide valuable clues.

March 25th

Don’t wait. The Relatives at RootsTech tool is only available until March 25th. It will take you some time to review the lists, but it’s fun because it’s like mining for buried ancestral gold nuggets. Except it’s not just a game. There is real genealogical gold hiding there, just itching to be discovered.

If you message someone, or click on the contact button, they will be added to your list which remains available after March 25th.

Do you have ancestors whose Y DNA and mitochondrial DNA you need? Your gold-nugget cousin may be waiting for you!

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Top Ten RootsTech 2022 DNA Sessions + All DNA Session Links

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The official dates of RootsTech 2022 were March 3-5, but the sessions and content in the vendor booths are still available. I’ve compiled a list of the sessions focused on DNA, with web links on the RootsTech YouTube channel

YouTube reports the number of views, so I was able to compile that information as of March 8, 2022.

I do want to explain a couple of things to add context to the numbers.

Most speakers recorded their sessions, but a few offered live sessions which were recorded, then posted later for participants to view. However, there have been glitches in that process. While the sessions were anticipated to be available an hour or so later, that didn’t quite happen, and a couple still aren’t posted. I’m sure the presenters are distressed by this, so be sure to watch those when they are up and running.

The Zoom rooms where participants gathered for the live sessions were restricted to 500 attendees. The YouTube number of views does not include the number of live viewers, so you’ll need to add an additional number, up to 500.

When you see a number before the session name, whether recorded or live, that means that the session is part of a series. RootsTech required speakers to divide longer sessions into a series of shorter sessions no longer than 15-20 minutes each. The goal was for viewers to be able to watch the sessions one after the other, as one class, or separately, and still make sense of the content. Let’s just say this was the most challenging thing I’ve ever done as a presenter.

For recorded series sessions, these are posted as 1, 2 and 3, as you can see below with Diahan Southard’s sessions. However, with my live session series, that didn’t happen. It looks like my sessions are a series, but when you watch them, parts 1, 2 and 3 are recorded and presented as one session. Personally, I’m fine with this, because I think the information makes a lot more sense this way. However, it makes comparisons difficult.

This was only the second year for RootsTech to be virtual and the conference is absolutely HUGE, so live and learn. Next year will be smoother and hopefully, at least partially in-person too.

When I “arrived” to present my live session, “Associating Autosomal DNA Segments With Ancestors,” my lovely moderator, Rhett, told me that they were going to livestream my session to the RootsTech page on Facebook as well because they realized that the 500 Zoom seat limit had been a problem the day before with some popular sessions. I have about 9000 views for that session and more than 7,400 of them are on the RootsTech Facebook page – and that was WITHOUT any advance notice or advertising. I know that the Zoom room was full in addition. I felt kind of strange about including my results in the top ten because I had that advantage, but I didn’t know quite how to otherwise count my session. As it turns out, all sessions with more than 1000 views made it into the top ten so mine would have been there one way or another. A big thank you to everyone who watched!

I hope that the RootsTech team notices that the most viewed session is the one that was NOT constrained by the 500-seat limited AND was live-streamed on Facebook. Seems like this might be a great way to increase session views for everyone next year. Hint, hint!!!

I also want to say a huge thank you to all of the presenters for producing outstanding content. The sessions were challenging to find, plus RootsTech is always hectic, even virtually. So, I know a LOT of people will want to view these informative sessions, now that you know where to look and have more time. Please remember to “like” the session on YouTube as a way of thanking your presenter.

With 140 DNA-focused sessions available, you can watch a new session, and put it to use, every other day for the next year! How fun is that! You can use this article as your own playlist.

Please feel free to share this article with your friends and genealogy groups so everyone can learn more about using DNA for genealogy.

Ok, let’s look at the top 10. Drum roll please…

Top 10 Most Viewed RootsTech Sessions

Session Title Presenter YouTube Link Views
1 1. Associating Autosomal DNA Segments With Ancestors Roberta Estes (live) https://www.youtube.com/watch?v=_IHSCkNnX48

 

 ~9000: 1019 + 500 live viewers + 7,400+ Facebook
2 1. What to Do with Your DNA Test Results in 2022 (part 1 of 3) Diahan Southard https://www.youtube.com/watch?v=FENAKAYLXX4 7428
3 Who Is FamilyTreeDNA? FamilyTreeDNA – Bennett Greenspan https://www.youtube.com/watch?v=MHFtwoatJ-A 2946
4 2. What to Do with Your DNA Test Results in 2022 (part 2 of 3) Diahan Southard https://www.youtube.com/watch?v=mIllhtONhlI 2448
5 Latest DNA Painter Releases DNAPainter Jonny Perl (live) https://www.youtube.com/watch?v=iLBThU8l33o 2230 + live viewers
6 DNA Painter Introduction DNAPainter – Jonny Perl https://www.youtube.com/watch?v=Rpe5LMPNmf0 1983
7 3. What to Do with Your DNA Test Results in 2022 (part 3 of 3) Diahan Southard https://www.youtube.com/watch?v=hemY5TuLmGI 1780
8 The Tree of Mankind Age Estimates Paul Maier https://www.youtube.com/watch?v=jjkL8PWAEwk 1638
9 A Sneak Peek at FamilyTreeDNA Coming Attractions FamilyTreeDNA (live) https://www.youtube.com/watch?v=K9sKqNScvnE 1270 + live viewers

 
10 Extending Time Horizons with DNA Rob Spencer (live) https://www.youtube.com/watch?v=wppXD1Zz2sQ 1037 + live viewers

 

All DNA-Focused Sessions

I know you’ll find LOTS of goodies here. Which ones are your favorites?

  Session Presenter YouTube Link Views
1 Estimating Relationships by Combining DNA from Multiple Siblings Amy Williams https://www.youtube.com/watch?v=xs1U0ohpKSA 201
2 Overview of HAPI-DNA.org Amy Williams https://www.youtube.com/watch?v=FjNiJgWaBeQ 126
3 How do AncestryDNA® Communities help tell your story? | Ancestry® Ancestry https://www.youtube.com/watch?v=EQNpUxonQO4 183

 
4 AncestryDNA® 201 Ancestry – Crista Cowan https://www.youtube.com/watch?v=lbqpnXloM5s

 

 494
5 Genealogy in a Minute: Increase Discoveries by Attaching AncestryDNA® Results to Family Tree Ancestry – Crista Cowan https://www.youtube.com/watch?v=iAqwSCO8Pvw 369
6 AncestryDNA® 101: Beginner’s Guide to AncestryDNA® | Ancestry® Ancestry – Lisa Elzey https://www.youtube.com/watch?v=-N2usCR86sY 909
7 Hidden in Plain Sight: Free People of Color in Your Family Tree Cheri Daniels https://www.youtube.com/watch?v=FUOcdhO3uDM 179
8 Finding Relatives to Prevent Hereditary Cancer ConnectMyVariant – Dr. Brian Shirts https://www.youtube.com/watch?v=LpwLGgEp2IE 63
9 Piling on the chromosomes Debbie Kennett https://www.youtube.com/watch?v=e14lMsS3rcY 465
10 Linking Families With Rare Genetic Condition Using Genealogy Deborah Neklason https://www.youtube.com/watch?v=b94lUfeAw9k 43
11 1. What to Do with Your DNA Test Results in 2022 Diahan Southard https://www.youtube.com/watch?v=FENAKAYLXX4 7428
12 1. What to Do with Your DNA Test Results in 2022 Diahan Southard https://www.youtube.com/watch?v=hemY5TuLmGI 1780
13 2. What to Do with Your DNA Test Results in 2022 Diahan Southard https://www.youtube.com/watch?v=mIllhtONhlI 2448
14 DNA Testing For Family History Diahan Southard https://www.youtube.com/watch?v=kCLuOCC924s 84

 
15 Understanding Your DNA Ethnicity Estimate at 23andMe Diana Elder

 

 https://www.youtube.com/watch?v=xT1OtyvbVHE 66
16 Understanding Your Ethnicity Estimate at FamilyTreeDNA Diana Elder https://www.youtube.com/watch?v=XosjViloVE0 73
17 DNA Monkey Wrenches Katherine Borges https://www.youtube.com/watch?v=Thv79pmII5M 245
18 Advanced Features in your Ancestral Tree and Fan Chart DNAPainter – Jonny Perl https://www.youtube.com/watch?v=4u5Vf13ZoAc 425
19 DNA Painter Introduction DNAPainter – Jonny Perl https://www.youtube.com/watch?v=Rpe5LMPNmf0 1983
20 Getting Segment Data from 23andMe DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=8EBRI85P3KQ 134
21 Getting segment data from FamilyTreeDNA DNA matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=rWnxK86a12U 169
22 Getting segment data from Gedmatch DNA matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=WF11HEL8Apk 163
23 Getting segment data from Geneanet DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=eclj8Ap0uK4 38
24 Getting segment data from MyHeritage DNA matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=9rGwOtqbg5E 160
25 Inferred Chromosome Mapping: Maximize your DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=tzd5arHkv64 688
26 Keeping track of your genetic family tree in a fan chart DNAPainter – Jonny Perl https://www.youtube.com/watch?v=W3Hcno7en94 806

 
27 Mapping a DNA Match in a Chromosome Map DNAPainter – Jonny Perl https://www.youtube.com/watch?v=A61zQFBWaiY 423
28 Setting up an Ancestral Tree and Fan Chart and Exploring Tree Completeness DNAPainter – Jonny Perl https://www.youtube.com/watch?v=lkJp5Xk1thg 77
29 Using the Shared cM Project Tool to Evaluate DNA Matches DNAPainter – Jonny Perl https://www.youtube.com/watch?v=vxhn9l3Dxg4 763
30 Your First Chromosome Map: Using your DNA Matches to Link Segments to Ancestors DNAPainter – Jonny Perl https://www.youtube.com/watch?v=tzd5arHkv64 688
31 DNA Painter for absolute beginners DNAPainter (Jonny Perl) https://www.youtube.com/watch?v=JwUWW4WHwhk 1196
32 Latest DNA Painter Releases DNAPainter (live) https://www.youtube.com/watch?v=iLBThU8l33o 2230 + live viewers
33 Unraveling your genealogy with DNA segment networks using AutoSegment from Genetic Affairs Evert-Jan Blom https://www.youtube.com/watch?v=rVpsJSqOJZI

 

 162
34 Unraveling your genealogy with genetic networks using AutoCluster Evert-Jan Blom https://www.youtube.com/watch?v=ZTKSz_X7_zs 201

 

 
35 Unraveling your genealogy with reconstructed trees using AutoTree & AutoKinship from Genetic Affairs Evert-Jan Blom https://www.youtube.com/watch?v=OmDQoAn9tVw 143
36 Research Like a Pro with DNA – A Genealogist’s Guide to Finding and Confirming Ancestors with DNA Family Locket Genealogists https://www.youtube.com/watch?v=NYpLscJJQyk 183
37 How to Interpret a DNA Network Graph Family Locket Genealogists – Diana Elder https://www.youtube.com/watch?v=i83WRl1uLWY 393
38 Find and Confirm Ancestors with DNA Evidence Family Locket Genealogists – Nicole Dyer https://www.youtube.com/watch?v=DGLpV3aNuZI 144
39 How To Make A DNA Network Graph Family Locket Genealogists – Nicole Dyer https://www.youtube.com/watch?v=MLm_dVK2kAA 201
40 Create A Family Tree With Your DNA Matches-Use Lucidchart To Create A Picture Worth A Thousand Words Family Locket Genealogists – Robin Wirthlin https://www.youtube.com/watch?v=RlRIzcW-JI4 270
41 Charting Companion 7 – DNA Edition Family Tree Maker https://www.youtube.com/watch?v=k2r9rkk22nU 316

 
42 Family Finder Chromosome Browser: How to Use FamilyTreeDNA https://www.youtube.com/watch?v=w0_tgopBn_o 750

 

 
43 FamilyTreeDNA: 22 Years of Breaking Down Brick Walls FamilyTreeDNA https://www.familysearch.org/rootstech/session/familytreedna-22-years-of-breaking-down-brick-walls Not available
44 Review of Autosomal DNA, Y-DNA, & mtDNA FamilyTreeDNA  – Janine Cloud https://www.youtube.com/watch?v=EJoQVKxgaVY 77
45 Who Is FamilyTreeDNA? FamilyTreeDNA – Bennett Greenspan https://www.youtube.com/watch?v=MHFtwoatJ-A 2946
46 Part 1: How to Interpret Y-DNA Results, A Walk Through the Big Y FamilyTreeDNA – Casimir Roman https://www.youtube.com/watch?v=ra1cjGgvhRw 684

 
47 Part 2: How to Interpret Y-DNA Results, A Walk Through the Big Y FamilyTreeDNA – Casimir Roman https://www.youtube.com/watch?v=CgqcjBD6N8Y

 

 259
48 Big Y-700: A Brief Overview FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=IefUipZcLCQ 96
49 Mitochondrial DNA & The Million Mito Project FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=5Zppv2uAa6I 179
50 Mitochondrial DNA: What is a Heteroplasmy FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=ZeGTyUDKySk 57
51 Y-DNA Big Y: A Lifetime Analysis FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=E6NEU92rpiM 154
52 Y-DNA: How SNPs Are Added to the Y Haplotree FamilyTreeDNA – Janine Cloud https://www.youtube.com/watch?v=CGQaYcroRwY 220
53 Family Finder myOrigins: Beginner’s Guide FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=VrJNpSv8nlA 88
54 Mitochondrial DNA: Matches Map & Results for mtDNA FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=YtA1j01MOvs 190
55 Mitochondrial DNA: mtDNA Mutations Explained FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=awPs0cmZApE 340

 
56 Y-DNA: Haplotree and SNPs Page Overview FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=FOuVhoMD-hw 432
57 Y-DNA: Understanding the Y-STR Results Page FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=gCeZz1rQplI 148
58 Y-DNA: What Is Genetic Distance? FamilyTreeDNA – Katy Rowe https://www.youtube.com/watch?v=qJ6wY6ILhfg 149
59 DNA Tools: myOrigins 3.0 Explained, Part 1 FamilyTreeDNA – Paul Maier https://www.youtube.com/watch?v=ACgY3F4-w78 74

 
60 DNA Tools: myOrigins 3.0 Explained, Part 2 FamilyTreeDNA – Paul Maier https://www.youtube.com/watch?v=h7qU36bIFg0 50
61 DNA Tools: myOrigins 3.0 Explained, Part 3 FamilyTreeDNA – Paul Maier https://www.youtube.com/watch?v=SWlGPm8BGyU 36
62 African American Genealogy Research Tips FamilyTreeDNA – Sherman McRae https://www.youtube.com/watch?v=XdbkM58rXIQ 153

 
63 Connecting With My Ancestors Through Y-DNA FamilyTreeDNA – Sherman McRae https://www.youtube.com/watch?v=xbo1XnLkuQU 200
64 Join The Million Mito Project FamilyTreeDNA (Join link) https://www.familysearch.org/rootstech/session/join-the-million-mito-project link
65 View the World’s Largest mtDNA Haplotree FamilyTreeDNA (Link to mtDNA tree) https://www.familytreedna.com/public/mt-dna-haplotree/L n/a
66 View the World’s Largest Y Haplotree FamilyTreeDNA (Link to Y tree) https://www.familytreedna.com/public/y-dna-haplotree/A link
67 A Sneak Peek at FamilyTreeDNA Coming Attractions FamilyTreeDNA (live) https://www.youtube.com/watch?v=K9sKqNScvnE 1270 + live viewers

 
68 DNA Upload: How to Transfer Your Autosomal DNA Data FamilyTreeDNA -Katy Rowe https://www.youtube.com/watch?v=CS-rH_HrGlo 303
69 Family Finder myOrigins: How to Compare Origins With Your DNA Matches FamilyTreeDNA -Katy Rowe https://www.youtube.com/watch?v=7mBmWhM4j9Y 145
70 Join Group Projects at FamilyTreeDNA FamilyTreeDNA link to learning center article) https://www.familysearch.org/rootstech/session/join-group-projects-at-familytreedna link

 
71 Product Demo – Unraveling your genealogy with reconstructed trees using AutoKinship GEDmatch https://www.youtube.com/watch?v=R7_W0FM5U7c 803
72 Towards a Genetic Genealogy Driven Irish Reference Genome Gerard Corcoran https://www.youtube.com/watch?v=6Kx8qeNiVmo 155

 
73 Discovering Biological Origins in Chile With DNA: Simple Triangulation Gonzalo Alexis Luengo Orellana https://www.youtube.com/watch?v=WcVby54Uigc 40
74 Cousin Lynne: An Adoption Story International Association of Jewish Genealogical Societies https://www.youtube.com/watch?v=AptMcV4_B4o 111
75 Using DNA Testing to Uncover Native Ancestry Janine Cloud https://www.youtube.com/watch?v=edzebJXepMA 205
76 1. Forensic Genetic Genealogy Jarrett Ross https://www.youtube.com/watch?v=0euIDZTmx5g 58
77 Reunited and it Feels so Good Jennifer Mendelsohn https://www.youtube.com/watch?v=X-hxjm7grBE 57

 
78 Genealogical Research and DNA Testing: The Perfect Companions Kimberly Brown https://www.youtube.com/watch?v=X82jA3xUVXk 80
79 Finding a Jewish Sperm Donor Kitty Munson Cooper https://www.youtube.com/watch?v=iKRjFfNcpug 164
80 Using DNA in South African Genealogy Linda Farrell https://www.youtube.com/watch?v=HXkbBWmORM0 141
81 Using DNA Group Projects In Your Family History Research Mags Gaulden https://www.youtube.com/watch?v=0tX7QDib4Cw 165
82 2. The Expansion of Genealogy Into Forensics Marybeth Sciaretta https://www.youtube.com/watch?v=HcEO-rMe3Xo 35

 
83 DNA Interest Groups That Keep ’em Coming Back McKell Keeney (live) https://www.youtube.com/watch?v=HFwpmtA_QbE 180 plus live viewers
84 Searching for Close Relatives with Your DNA Results Mckell Keeney (live) https://www.familysearch.org/rootstech/session/searching-for-close-relatives-with-your-dna-results Not yet available
85 Top Ten Reasons To DNA Test For Family History Michelle Leonard https://www.youtube.com/watch?v=1B9hEeu_dic 181
86 Top Tips For Identifying DNA Matches Michelle Leonard https://www.youtube.com/watch?v=-3Oay_btNAI 306
87 Maximising Messages Michelle Patient https://www.youtube.com/watch?v=4TRmn0qzHik 442
88 How to Filter and Sort Your DNA Matches MyHeritage https://www.youtube.com/watch?v=fmIgamFDvc8 88
89 How to Get Started with Your DNA Matches MyHeritage https://www.youtube.com/watch?v=JPOzhTxhU0E 447

 
90 How to Track DNA Kits in MyHeritage` MyHeritage https://www.youtube.com/watch?v=2W0zBbkBJ5w 28

 
91 How to Upload Your DNA Data to MyHeritage MyHeritage https://www.youtube.com/watch?v=nJ4RoZOQafY 82
92 How to Use Genetic Groups MyHeritage https://www.youtube.com/watch?v=PtDAUHN-3-4 62
My Story: Hope MyHeritage https://www.youtube.com/watch?v=qjyggKZEXYA 133
93 MyHeritage Keynote, RootsTech 2022 MyHeritage https://www.familysearch.org/rootstech/session/myheritage-keynote-rootstech-2022 Not available
94 Using Labels to Name Your DNA Match List MyHeritage https://www.youtube.com/watch?v=enJjdw1xlsk 139

 
95 An Introduction to DNA on MyHeritage MyHeritage – Daniel Horowitz https://www.youtube.com/watch?v=1I6LHezMkgc 60
96 Using MyHeritage’s Advanced DNA Tools to Shed Light on Your DNA Matches MyHeritage – Daniel Horowitz https://www.youtube.com/watch?v=Pez46Xw20b4 110
97 You’ve Got DNA Matches! Now What? MyHeritage – Daniel Horowitz https://www.youtube.com/watch?v=gl3UVksA-2E 260
98 My Story: Lizzie and Ayla MyHeritage – Elizbeth Shaltz https://www.youtube.com/watch?v=NQv6C8G39Kw 147
99 My Story: Fernando and Iwen MyHeritage – Fernando Hermansson https://www.youtube.com/watch?v=98-AR0M7fFE 165

 
100 Using the Autocluster and the Chromosome Browser to Explore Your DNA Matches MyHeritage – Gal Zruhen https://www.youtube.com/watch?v=a7aQbfP7lWU 115

 
101 My Story : Kara Ashby Utah Wedding MyHeritage – Kara Ashby https://www.youtube.com/watch?v=Qbr_gg1sDRo 200
102 When Harry Met Dotty – using DNA to break down brick walls Nick David Barratt https://www.youtube.com/watch?v=8SdnLuwWpJs 679
103 How to Add a DNA Match to Airtable Nicole Dyer https://www.youtube.com/watch?v=oKxizWIOKC0 161
104 How to Download DNA Match Lists with DNAGedcom Client Nicole Dyer https://www.youtube.com/watch?v=t9zTWnwl98E 124
105 How to Know if a Matching DNA Segment is Maternal or Paternal Nicole Dyer https://www.youtube.com/watch?v=-zd5iat7pmg 161
106 DNA Basics Part I Centimorgans and Family Relationships Origins International, Inc. dba Origins Genealogy https://www.youtube.com/watch?v=SI1yUdnSpHA 372
107 DNA Basics Part II Clustering and Connecting Your DNA Matches Origins International, Inc. dba Origins Genealogy https://www.youtube.com/watch?v=ECs4a1hwGcs 333
108 DNA Basics Part III Charting Your DNA Matches to Get Answers Origins International, Inc. dba Origins Genealogy https://www.youtube.com/watch?v=qzybjN0JBGY 270
109 2. Using Cluster Auto Painter Patricia Coleman https://www.youtube.com/watch?v=-nfLixwxKN4 691
110 3. Using Online Irish Records Patricia Coleman https://www.youtube.com/watch?v=mZsB0l4z4os 802
111 Exploring Different Types of Clusters Patricia Coleman https://www.youtube.com/watch?v=eEZBFPC8aL4 972

 
112 The Million Mito Project: Growing the Family Tree of Womankind Paul Maier https://www.youtube.com/watch?v=cpctoeKb0Kw 541
113 The Tree of Mankind Age Estimates Paul Maier https://www.youtube.com/watch?v=jjkL8PWAEwk 1638
114 Y-DNA and Mitochondrial DNA Testing Plans Paul Woodbury https://www.youtube.com/watch?v=akymSm0QKaY 168
115 Finding Biological Family Price Genealogy https://www.youtube.com/watch?v=4xh-r3hZ6Hw 137
116 What Y-DNA Testing Can Do for You Richard Hill https://www.youtube.com/watch?v=a094YhIY4HU 191
117 Extending Time Horizons with DNA Rob Spencer (live) https://www.youtube.com/watch?v=wppXD1Zz2sQ 1037 + live viewers
118 DNA for Native American Ancestry by Roberta Estes Roberta Estes https://www.youtube.com/watch?v=EbNyXCFfp4M 212
119 1. Associating Autosomal DNA Segments With Ancestors Roberta Estes (live) https://www.youtube.com/watch?v=_IHSCkNnX48

 

 ~9000: 1019 + 500 live viewers + 7,400+ Facebook
120 1. What Can I Do With Ancestral DNA Segments? Roberta Estes (live) https://www.youtube.com/watch?v=Suv3l4iZYAQ 325 plus live viewers

 
121 Native American DNA – Ancient and Contemporary Maps Roberta Estes (live) https://www.youtube.com/watch?v=dFTl2vXUz_0 212 plus 483 live viewers

 
122 How Can DNA Enhance My Family History Research? Robin Wirthlin https://www.youtube.com/watch?v=f3KKW-U2P6w 102
123 How to Analyze a DNA Match Robin Wirthlin https://www.youtube.com/watch?v=LTL8NbpROwM 367
124 1. Jewish Ethnicity & DNA: History, Migration, Genetics Schelly Talalay Dardashti https://www.youtube.com/watch?v=AIJyphGEZTA 82

 
125 2. Jewish Ethnicity & DNA: History, Migration, Genetics Schelly Talalay Dardashti https://www.youtube.com/watch?v=VM3MCYM0hkI 72
126 Ask us about DNA Talking Family History (live) https://www.youtube.com/watch?v=kv_RfR6OPpU 96 plus live viewers
127 1. An Introduction to Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=WNhErW5UVKU

 

 183
128 2. An Introduction to Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=CRpQ8EVOShI 110

 
129 Common Problems When Doing Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=hzFxtBS5a8Y 68
130 Cross Visual Phasing to Go Back Another Generation Tanner Blair Tolman https://www.youtube.com/watch?v=MrrMqhfiwbs 64
131 DNA Basics Tanner Blair Tolman https://www.youtube.com/watch?v=OCMUz-kXNZc 155
132 DNA Painter and Visual Phasing Tanner Blair Tolman https://www.youtube.com/watch?v=2-eh1L4wOmQ 155
133 DNA Painter Part 2: Chromosome Mapping Tanner Blair Tolman https://www.youtube.com/watch?v=zgOJDRG7hJc 172
134 DNA Painter Part 3: The Inferred Segment Generator Tanner Blair Tolman https://www.youtube.com/watch?v=96ai8nM4lzo

 

 100
135 DNA Painter Part 4: The Distinct Segment Generator Tanner Blair Tolman https://www.youtube.com/watch?v=Pu-WIEQ_8vc 83
136 DNA Painter Part 5: Ancestral Trees Tanner Blair Tolman https://www.youtube.com/watch?v=dkYDeFLduKA 73
137 Understanding Your DNA Ethnicity Results Tanner Blair Tolman https://www.youtube.com/watch?v=4tAd8jK6Bgw 518
138 What’s New at GEDmatch Tim Janzen https://www.youtube.com/watch?v=AjA59BG_cF4

 

 515
139 What Does it Mean to Have Neanderthal Ancestry? Ugo Perego https://www.youtube.com/watch?v=DshCKDW07so 190
140 Big Y-700 Your DNA Guide https://www.youtube.com/watch?v=rIFC69qswiA 143
141 Next Steps with Your DNA Your DNA Guide – Diahan Southard (live) https://www.familysearch.org/rootstech/session/next-steps-with-your-dna Not yet available

Additions:

142  Adventures of an Amateur Genetic Genealogist – Geoff Nelson https://www.familysearch.org/rootstech/session/adventures-of-an-amateur-genetic-genealogist     291 views

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