Mitochondrial DNA – Your Mom’s Story

Have you ever wondered why you would want to test your mitochondrial DNA? What would a mitochondrial DNA test tell you about your ancestors? What would it mean to you and how would it help your genealogy?

If you’re like most genealogists, you want to know every single tidbit you can discover about your ancestors – and mitochondrial DNA not only tells us about people we match that are currently living, that share ancestors with us at some point in time, but it also reaches back beyond the range of what genealogy in the traditional sense can tell us – past the time when surnames were adopted, peering into the misty veil of the past!

Every single one of your ancestors has their own individual story to tell – and if you really want to know who you are and where each ancestral line came from, mitochondrial DNA is the insider story on your mother’s matrilineal line.

What Is Mitochondrial DNA?

Mitochondrial DNA a special type of DNA that tells the direct line story of your mother’s mother’s mother’s heritage – all the way back as far as we can go – beyond genealogy– to the woman from whom we are all descended that we call “mitochondrial Eve.”

Mitochondrial DNA is never mixed with the father’s DNA, so the red circle pedigree line above remains unbroken and intact and is passed from mothers to all of their children, as you can see in the brother and sister at the bottom. Only females pass mitochondrial DNA on to their children, so all children carry their mother’s mtDNA. The great news is that everyone can test for mitochondrial DNA, unlike Y DNA where only males can test, shown by the blue square pedigree line above.

However, because of the surname changes in every generation for females, you can’t tell at a glance by looking at your mitochondrial matches’ surname if you are or might be related, like you can with Y DNA which tracks the direct paternal line which means the surname typically doesn’t change. If your match doesn’t list a common ancestor that you recognize, you may need to do some genealogy work to search for that ancestor.

This doesn’t mean mitochondrial DNA isn’t useful, because it can provide you with lots of information – some of which is useful genealogically and some that provides you with knowledge of where your matrilineal line came from and their course of travel through time, over hundreds and thousands of years.

Mitochondrial DNA is an extremely underutilized resource that gives us the ability to peer down the periscope of one family line for thousands of years.

Not to mention, it’s just plain fun!  Who doesn’t want to know more about our ancestors, and especially when the information resides within us and is so easy to retrieve.

Family Tree DNA provides 10 great mitochondrial tools for every customer. Let’s take a look at what you receive and how to utilize this information.

Haplogroup

Everyone who tests their mitochondrial DNA at Family Tree DNA receives a haplogroup assignment. Think of a haplogroup as your genetic clan. Haplogroups have a history and a pedigree chart, just like people do. Haplogroups and their branches can identify certain groups of people, such as people of African descent, European, Asian, Jewish and Native American.

While the matrilineal DNA is passed intact with no admixture from the father, occasionally mutations do happen, and it’s those historical mutations that form clans and branches of clans as generation after generation is born and continues to migrate to new areas.

If you take the entry level mtDNA Plus test which only tests about 6% of the available mitochondrial markers, those most likely to mutate, you will receive a base haplogroup, because that’s all that can be determined by those markers. If you take the mtFull Sequence test which tests all of the 16,569 mitochondrial locations, you will receive a full haplogroup designation, plus a lot more.

What’s the difference? In my case, my full haplogroup is J1c2f, meaning that my branch of haplogroup J is the result of 4 branching events from mother haplogroup J. Haplogroup J itself was formed by a defining set of mutations. The first branch was J1, then J1c, and so forth.

Haplogroup J was formed someplace in the Middle East and its branches are found primarily in the Mediterranean, Europe and western Asia today, plus, of course, diaspora regions like the Americas, Canada, Australia and New Zealand.

The mitochondrial Haplogroup J Project at Family Tree DNA provides a map of the most distant known ancestors of Haplogroup J members, including all branches, shown below.

My branch, haplogroup J1c2f, a rare haplogroup, is found in a much more restricted geography. It has taken 10 years or so to accumulate 10 pins on the map.  Of course, there would be more if everyone tested and joined their haplogroup project.

How Old is Haplogroup J?

With the mtFull Sequence test, you receive a lot more information than with the mtPlus test, for not a lot more investment, as you can see in the chart below and as we work through results.

Haplogroup Born Years Ago Receive With Test
J 34,000 mtPlus
J1 27,000 mtFull Sequence
J1c 13.000 mtFull Sequence
J1c2 10,000 mtFull Sequence
J1c2f 1,000 mtFull Sequence

Estimated dates for each haplogroup branches “birth” have been provided in the paper, A “Copernican” Reassessment of the Human Mitochondrial DNA Tree from its Root by Behar et al.

Haplogroup J itself was born about 34,000 years ago, someplace in the Middle East or near the Black Sea.

Haplogroup J1c2f was born about 1000 years ago, and utilizing the map of J1c2f in combination with the known history of my full sequence matches allows me to learn where my ancestors were in more recent times. In my case, I’m fascinated by that cluster in Sweden and Norway, all of whom I’m related to with in the last 1000 years or so. Is there a message there for me about where my ancestor lived, perhaps, before the first documentation of my ancestral line in Germany in 1799?

More Please

Are you starting to see the benefit to mitochondrial DNA testing? We’ve only scratched the surface.

At Family Tree DNA, your haplogroup is shown in the upper right hand corner on your personal page dashboard.

In the mtDNA section, additional tools are shown. Let’s look at each one and what it can tell you about your matrilineal line.

Please note that you can click to enlarge any image.

You can always navigate to the Dashboard or any other option by clicking on the myFTDNA button on the upper left hand corner.

Matches

The first place most people look is at their Matches page. In my case, I have 38 full sequence matches. Full sequence matches are the most likely to match in a genealogical time frame. You can see by just looking at my matches below why entering the name of your earliest known ancestor (under Manage Personal Information, Account Settings, Genealogy) is so important!!! That’s the first thing people see and the best indication of a common ancestor. I always include a name, birth/death date and location.

As you can see, most of my matches (names obscured for privacy) have trees attached to their results and many have also taken the family finder test. Both are great news for me!

I can then view at my HVR1+HVR2 matches, which is equivalent to the mtPlus test today.

I have 266 HVR1+HVR2 matches, many of whom have also taken the full sequence test. Those who have taken the higher level test, I can disregard because their results, if they match, are already included on the full sequence match page. I do review the people who have not yet taken the full sequence test because a valuable match may be lurking there.

I can e-mail my matches by clicking on the envelope.

Results

Next, let’s look at our results. This page should really probably say “raw results,” because as many people say, “it’s just a page of numbers.”  Yes, it is, but there is magic in these numbers because they are the key to “everything else mitochondrial.”

This page shows your mutations – in other words, what makes you both different from other people and the same as people you match, which isolates your matches to people with whom you share a common ancestor at some point in time. The fewer mutations difference, generally the closer in time your common ancestor. If you match someone exactly, it means you share all of the same mutations, including “extra” and “missing” mutations typically found in people who carry your hapologroup.

There are two formats provided, the RSRS and the CRS, which I explained in the article, The CRS and the RSRS. You don’t need to know these details, but they are available if you are interested.

Some of these mutations shown are your haplogroup and subgroup defining mutations. For example, haplogroup J1c2f is defined by the mutation at location 9055, shown above. If you have all these mutations but don’t have G9055A, then you’re not haplgroup J1c2f, you’re J1c2.

Haplogroup Haplogroup Defining Mutations
J C295T, T489C, A10398G!, A12612G, G13708A, C16069T
J1 C462T, G3010A
J1c T14798C
J1c2 A188G
J1c2f G9055A

Most mutations shown, other than haplogroup defining mutations, are typically found in your subgroup, but others are “rare.” It’s those rare extra or missing mutations that are your family-line-defining mutations. In my case, both G185A and G228A are family line defining. But you really don’t need to worry about this unless you are going to take a deep dive, because the matching and other tools included by Family Tree DNA provide further analysis in ways far easier to understand and without you having to understand or worry about the nitty-gritty details.

The beauty of these numbers, is, of course, in the underlying story they tell us. You can’t have matches without these numbers. You also can’t have maps or anything else without the raw mutation information.

Let’s look at the story they tell.

Matches Maps

One of my favorite tools is the Matches Map because it shows the most distant ancestor for all of your matches that have provided that information.

Hint: You MUST enter the geographic information through the “Update Ancestor’s Location” link at the bottom of this map for YOUR ancestor to be displayed on THIS map (white pin) and also on the maps of your matches. You can see how useful this information is!  I wish everyone would do this, even if they are adopted and the only information they have is where they were born! Clusters are important for genealogy matching as well as for more distant origins.

You can also display your match list by clicking on the “Show Match List” link under the map. You can click on the pins on the map to display the accompanying information.

On the full sequence map, your exact matches are shown in red, 1 step mutations in orange, 2 steps in yellow, so you can easily look for clusters.

Once again, the Scandinavian group stands out because many are exact matches to my German ancestor. Do you think there might be a message there?

If not for my mitochondrial DNA, how else would I ever obtain this information, given that the German church records ended in 1799 for my matrilineal line? Did they end in 1799 because my ancestral line wasn’t in Germany before that?

Migration and Frequency Maps

Are you wondering how your ancestor and her ancestors arrived in the location where you first find them?

The haplogroup Migration Maps show you the ancestral path from Africa to, in my case, Europe.

The Frequency Map then shows you how much of the European population is haplogroup J, which includes subgroups.

Haplogroup Origins

The Haplogroup Origins page shows me the distribution of my haplogroup, by region, by match type.

For example, I have 7 exact matches in Norway and 1 in Poland. Only a portion of my Haplogroup Origins page is shown here, and only the Full Sequence Matches.  HVR1 and HVR1+HVR2 matches are displayed as well.

Ancestral Origins

The Ancestral Origins page shows my matches by Country along with any comments. My matches shown don’t have any comments, but comments might be Ashkenazi or MDKO (most distant known origin) when US is given as the most distant ancestral location.

Again, I’ve only shown my full sequence matches.

Advanced Matching Combines Tools

Another of my favorite tools is Advanced Matches, available under the Tools and Apps tab.

Advanced Matches is a wonderful tool that allows you to combine test types. For example, let’s say that you want to know if any of the people you match on the mtDNA test are also showing up as a match on the Family Finder test. You could further limit this by project as well.

Be sure to click on “show only people I match in all selected tests” or you’ll receive the combined list of all matches, not just the people who match on BOTH tests, which is what you want.

There aren’t any people that match me on BOTH the Family Finder test and the full sequence mtDNA test, which tells me that these matches are several generations back in time.  For purposes of example, I’m showing my two matches on both the HVR1 and the Family Finder test, below – just so you can see how the tool works.

Because both of these people tested at the HVR2 level, where we don’t match, the mitochondrial part of this match is likely hundreds to thousands of years ago and isn’t connected to the Family Finder match.  However, if these two matches had NOT tested at a higher level, where I know we don’t match, the combined match of mtDNA and the Family Finder test might be a significant hint as to our common ancestral line.

Of course, for adoptees, finding someone with whom you match closely on the Family Finder test AND match exactly on the full sequence test would be very suggestive of a matrilineal common ancestor in a recent timeframe.

Combination matching is a powerful tool.

Projects

We started our discussion about mitochondrial haplogroups by referencing the MtDNA Haplogroup J project. Family Tree DNA has over 9000 projects for you to select from.

Thankfully, you don’t have to browse through them all, as they are broken down into categories.

  • Haplogroup projects are categorized by Y or mtDNA and then by subhaplogroup where appropriate.
  • Surname projects exist as well and are searchable for your genealogy lines.
  • Geographical projects cover everything else, from geographies such as the Cumberland Gap region of Appalachia to the American Indian project. Some projects focus on Y DNA, some on mtDNA, some both plus include people with autosomal results that pertain to that project.

Project administrators can enter surnames that pertain to their project so that Family Tree DNA can match the tester’s surname to the project list to provide the tester with a menu.

Please do READ the project description before joining, as lot of people join every project listed, even though the surname listed in that project in no way pertains to their family.  For example, in the Estes list above, my Estes line is in no way connected to the Estis family of the Ukraine or Fairfield County, SC nor are they haplogroup I, so joining the haplogroup I-L161(Isles) Y DNA project would be futile even if I was an Estes male.

Needless to say, if you’re a female who did not test under your birth surname, the project menu won’t be relevant to you, so you’ll need to use the “Search by Surname” function, at the bottom of the menu to find projects for your surname.

You can also scroll down and browse in a number of ways, in addition to surname.

All testers should join their haplogroup project so that everyone can benefit from collaboration. Testing in isolation without collaboration benefits no one.  We all benefit from matching and sharing, both individually and as a larger group.  Think of those maps and clusters!

You can join and manage your projects from your home page by clicking on the Projects tab on the upper left.

Mitochondrial Summary

I hope this overview has provided you with some good reasons to test your mitochondrial DNA or to better understand your results if you’ve already tested.

Mitochondrial DNA holds the secrets of your matrilineal line. You never know what you don’t know unless you test. You don’t know what kind of surprises are waiting for you – and let’s face it, our ancestors are always full of surprises!

You can order or upgrade your mitochondrial DNA test by clicking here.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

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Which DNA Test is Best?

If you’re reading this article, congratulations. You’re a savvy shopper and you’re doing some research before purchasing a DNA test. You’ve come to the right place.

The most common question I receive is asking which test is best to purchase. There is no one single best answer for everyone – it depends on your testing goals and your pocketbook.

Testing Goals

People who want to have their DNA tested have a goal in mind and seek results to utilize for their particular purpose. Today, in the Direct to Consumer (DTC) DNA market space, people have varied interests that fall into the general categories of genealogy and medical/health.

I’ve approached the question of “which test is best” by providing information grouped into testing goal categories.  I’ve compared the different vendors and tests from the perspective of someone who is looking to test for those purposes – and I’ve created separate sections of this article for each interest..

We will be discussing testing for:

  • Ethnicity – Who Am I? – Breakdown by Various World Regions
  • Adoption – Finding Missing Parents or Close Family
  • Genealogy – Cousin Matching and Ancestor Search/Verification
  • Medical/Health

We will be reviewing the following test types:

  • Autosomal
  • Y DNA (males only)
  • Mitochondrial DNA

I have included summary charts for each section, plus an additional chart for:

  • Additional Vendor Considerations

If you are looking to select one test, or have limited funds, or are looking to prioritize certain types of tests, you’ll want to read about each vendor, each type of test, and each testing goal category.

Each category reports information about the vendors and their products from a different perspective – and only you can decide which of these perspectives and features are most important to you.

You might want to read this short article for a quick overview of the 4 kinds of DNA used for genetic genealogy and DTC testing and how they differ.

The Big 3

Today, there are three major players in the DNA testing market, not in any particular order:

Each of these companies offers autosomal tests, but each vendor offers features that are unique. Family Tree DNA and 23andMe offer additional tests as well.

In addition to the Big 3, there are a couple of new kids on the block that I will mention where appropriate. There are also niche players for the more advanced genetic genealogist or serious researcher, and this article does not address advanced research.

In a nutshell, if you are serious genealogist, you will want to take all of the following tests to maximize your tools for solving genealogical puzzles. There is no one single test that does everything.

  • Full mitochondrial sequence that informs you about your matrilineal line (only) at Family Tree DNA. This test currently costs $199.
  • Y DNA test (for males only) that informs you about your direct paternal (surname) line (only) at Family Tree DNA. This test begins at $169 for 37 markers.
  • Family Finder, an autosomal test that provides ethnicity estimates and cousin matching at Family Tree DNA. This test currently costs $89.
  • AncestryDNA, an autosomal test at Ancestry.com that provides ethnicity estimates and cousin matching. (Do not confuse this test with Ancestry by DNA, which is not the same test and does not provide the same features.) This test currently costs $99, plus the additional cost of a subscription for full feature access. You can test without a subscription, but nonsubscribers can’t access all of the test result features provided to Ancestry subscribers.
  • 23andMe Ancestry Service test, an autosomal test that provides ethnicity estimates and cousin matching. The genealogy version of this test costs $99, the medical+genealogy version costs $199.

A Word About Third Party Tools

A number of third party tools exist, such as GedMatch and DNAGedcom.com, and while these tools are quite useful after testing, these vendors don’t provide tests. In order to use these sites, you must first take an autosomal DNA test from a testing vendor. This article focuses on selecting your DNA testing vendor based on your testing goals.

Let’s get started!

Ethnicity

Many people are drawn to DNA testing through commercials that promise to ‘tell you who you are.” While the allure is exciting, the reality is somewhat different.

Each of the major three vendors provide an ethnicity estimate based on your autosomal DNA test, and each of the three vendors will provide you with a different result.

Yep, same person, different ethnicity breakdowns.

Hopefully, the outcomes will be very similar, but that’s certainly not always the case. However, many people take one test and believe those results wholeheartedly. Please don’t. You may want to read Concepts – Calculating Ethnicity Percentages to see how varied my own ethnicity reports are at various vendors as compared to my known genealogy.

The technology for understanding “ethnicity” from a genetic perspective is still very new. Your ethnicity estimate is based on reference populations from around the world – today. People and populations move, and have moved, for hundreds, thousands and tens of thousands of years. Written history only reaches back a fraction of that time, so the estimates provided to people today are not exact.

That isn’t to criticize any individual vendor. View each vendor’s results not as gospel, but as their opinion based on their reference populations and their internal proprietary algorithm of utilizing those reference populations to produce your ethnicity results.

To read more about how ethnicity testing works, and why your results may vary between vendors or not be what you expected, click here.

I don’t want to discourage anyone from testing, only to be sure consumers understand the context of what they will be receiving. Generally speaking, these results are accurate at the continental level, and less accurate within continents, such as European regional breakdowns.

All three testing companies provide additional features or tools, in addition to your ethnicity estimates, that are relevant to ethnicity or population groups.

Let’s look at each company separately.

Ethnicity – Family Tree DNA

Family Tree DNA’s ethnicity tool is called myOrigins and provides three features or tools in addition to the actual ethnicity estimate and associated ethnicity map.

Please note that throughout this article you can click on any image to enlarge.

On the myOrigins ethnicity map page, above, your ethnicity percentages and map are shown, along with two additional features.

The Shared Origins box to the left shows the matching ethnic components of people on your DNA match list. This is particularly useful if you are trying to discover, for example, where a particular minority admixture comes from in your lineage. You can select different match types, for example, immediate relatives or X chromosome matches, which have special inheritance qualities.

Clicking on the apricot (mitochondrial DNA) and green (Y DNA) pins in the lower right corner drops the pins in the locations on your map of the most distant ancestral Y and mitochondrial DNA locations of the individuals in the group you have selected in the Shared Origins match box. You may or may not match these individuals on the Y or mtDNA lines, but families tend to migrate in groups, so match hints of any kind are important.

A third unique feature provided by Family Tree DNA is Ancient Origins, a tool released with little fanfare in November 2016.

Ancient Origins shows the ancient source of your European DNA, based on genome sequencing of ancient DNA from the locations shown on the map.

Additionally, Family Tree DNA hosts an Ancient DNA project where they have facilitated the upload of the ancient genomes so that customers today can determine if they match these ancient individuals.

Kits included in the Ancient DNA project are shown in the chart below, along with their age and burial location. Some have matches today, and some of these samples are included on the Ancient Origins map.

Individual Approx. Age Burial Location Matches Ancient Origins Map
Clovis Anzick 12,500 Montana (US) Yes No
Linearbandkeramik 7,500 Stuttgart, Germany Yes Yes
Loschbour 8,000 Luxembourg Yes Yes
Palaeo-Eskimo 4,000 Greenland No No
Altai Neanderthal 50,000 Altai No No
Denisova 30,000 Siberia No No
Hinxton-4 2,000 Cambridgeshire, UK No No
BR2 3,200 Hungary Yes Yes
Ust’-Ishim 45,000 Siberia Yes No
NE1 7,500 Hungary Yes Yes

Ethnicity – Ancestry

In addition to your ethnicity estimate, Ancestry also provides a feature called Genetic Communities.

Your ethnicity estimate provides percentages of DNA found in regions shown on the map by fully colored shapes – green in Europe in the example above. Genetic Communities show how your DNA clusters with other people in specific regions of the world – shown with dotted clusters in the US in this example.

In my case, my ethnicity at Ancestry shows my European roots, illustrated by the green highlighted areas, and my two Genetic Communities are shown by yellow and red dotted regions in the United States.

My assigned Genetic Communities indicate that my DNA clusters with other people whose ancestors lived in two regions; The Lower Midwest and Virginia as well as the Alleghenies and Northeast Indiana.

Testers can then view their DNA matches within that community, as well as a group of surnames common within that community.

The Genetic Communities provided for me are accurate, but don’t expect all of your genealogical regions to be represented in Genetic Communities. For example, my DNA is 25% German, and I don’t have any German communities today, although ancestry will be adding new Genetic Communities as new clusters are formed.

You can read more about Genetic Communities here and here.

Ethnicity – 23andMe

In addition to ethnicity percentage estimates, called Ancestry Composition, 23andMe offers the ability to compare your Ancestry Composition against that of your parent to see which portions of your ethnicity you inherited from each parent, although there are problems with this tool incorrectly assigning parental segments.

Additionally, 23andMe paints your chromosome segments with your ethnic heritage, as shown below.

You can see that my yellow Native American segments appear on chromosomes 1 and 2.

In January 2017, 23andMe introduced their Ancestry Timeline, which I find to be extremely misleading and inaccurate. On my timeline, shown below, they estimate that my most recent British and Irish ancestor was found in my tree between 1900 and 1930 while in reality my most recent British/Irish individual found in my tree was born in England in 1759.

I do not view 23andMe’s Ancestry Timeline as a benefit to the genealogist, having found that it causes people to draw very misleading conclusions, even to the point of questioning their parentage based on the results. I wrote about their Ancestry Timeline here.

Ethnicity Summary

All three vendors provide both ethnicity percentage estimates and maps. All three vendors provide additional tools and features relevant to ethnicity. Vendors also provide matching to other people which may or may not be of interest to people who test only for ethnicity. “Who you are” only begins with ethnicity estimates.

DNA test costs are similar, although the Family Tree DNA test is less at $89. All three vendors have sales from time to time.

Ethnicity Vendor Summary Chart

Ethnicity testing is an autosomal DNA test and is available for both males and females.

Family Tree DNA Ancestry 23andMe
Ethnicity Test Included with $89 Family Finder test Included with $99 Ancestry DNA test Included with $99 Ancestry Service
Percentages and Maps Yes Yes Yes
Shared Ethnicity with Matches Yes No Yes
Additional Feature Y and mtDNA mapping of ethnicity matches Genetic Communities Ethnicity phasing against parent (has issues)
Additional Feature Ancient Origins Ethnicity mapping by chromosome
Additional Feature Ancient DNA Project Ancestry Timeline

 

Adoption and Parental Identity

DNA testing is extremely popular among adoptees and others in search of missing parents and grandparents.

The techniques used for adoption and parental search are somewhat different than those used for more traditional genealogy, although non-adoptees may wish to continue to read this section because many of the features that are important to adoptees are important to other testers as well.

Adoptees often utilize autosomal DNA somewhat differently than traditional genealogists by using a technique called mirror trees. In essence, the adoptee utilizes the trees posted online of their closest DNA matches to search for common family lines within those trees. The common family lines will eventually lead to the individuals within those common trees that are candidates to be the parents of the searcher.

Here’s a simplified hypothetical example of my tree and a first cousin adoptee match.

The adoptee matches me at a first cousin level, meaning that we share at least one common grandparent – but which one? Looking at other people the adoptee matches, or the adoptee and I both match, we find Edith Lore (or her ancestors) in the tree of multiple matches. Since Edith Lore is my grandmother, the adoptee is predicted to be my first cousin, and Edith Lore’s ancestors appear in the trees of our common matches – that tells us that Edith Lore is also the (probable) grandmother of the adoptee.

Looking at the possibilities for how Edith Lore can fit into the tree of me and the adoptee, as first cousins, we fine the following scenario.

Testing the known child of daughter Ferverda will then provide confirmation of this relationship if the known child proves to be a half sibling to the adoptee.

Therefore, close matches, the ability to contact matches and trees are very important to adoptees. I recommend that adoptees make contact with www.dnaadoption.com. The volunteers there specialize in adoptions and adoptees, provide search angels to help people and classes to teach adoptees how to utilize the techniques unique to adoption search such as building mirror trees.

For adoptees, the first rule is to test with all 3 major vendors plus MyHeritage. Family Tree DNA allows you to test with both 23andMe and Ancestry and subsequently transfer your results to Family Tree DNA, but I would strongly suggest adoptees test on the Family Tree DNA platform instead. Your match results from transferring to Family Tree DNA from other companies, except for MyHeritage, will be fewer and less reliable because both 23andMe and Ancestry utilize different chip technology.

For most genealogists, MyHeritage is not a player, as they have only recently entered the testing arena, have a very small data base, no tools and are having matching issues. I recently wrote about MyHeritage here. However, adoptees may want to test with MyHeritage, or upload your results to MyHeritage if you tested with Family Tree DNA, because your important puzzle-solving match just might have tested there and no place else. You can read about transfer kit compatibility and who accepts which vendors’ tests here.

Adoptees can benefit from ethnicity estimates at the continental level, meaning that regional (within continent) or minority ethnicity should be taken with a very large grain of salt. However, knowing that you have 25% Jewish heritage, for example, can be a very big clue to an adoptee’s search.

Another aspect of the adoptees search that can be relevant is the number of foreign testers. For many years, neither 23andMe, nor Ancestry tested substantially (or at all) outside the US. Family Tree DNA has always tested internationally and has a very strong Jewish data base component.

Not all vendors report X chromosome matches. The X chromosome is important to genetic genealogy, because it has a unique inheritance path. Men don’t inherit an X chromosome from their fathers. Therefore, if you match someone on the X chromosome, you know the relationship, for a male, must be from their mother’s side. For a female, the relationship must be from the mother or the father’s mother’s side. You can read more about X chromosome matching here.

Neither Ancestry nor MyHeritage have chromosome browsers which allow you to view the segments of DNA on which you match other individuals, which includes the X chromosome.

Adoptee Y and Mitochondrial Testing

In addition to autosomal DNA testing, adoptees will want to test their Y DNA (males only) and mitochondrial DNA.

These tests are different from autosomal DNA which tests the DNA you receive from all of your ancestors. Y and mitochondrial DNA focus on only one specific line, respectively. Y DNA is inherited by men from their fathers and the Y chromosome is passed from father to son from time immemorial. Therefore, testing the Y chromosome provides us with the ability to match to current people as well as to use the Y chromosome as a tool to look far back in time. Adoptees tend to be most interested in matching current people, at least initially.

Working with male adoptees, I have a found that about 30% of the time a male will match strongly to a particular surname, especially at higher marker levels. That isn’t always true, but adoptees will never know if they don’t test. An adoptee’s match list is shown at 111 markers, below.

Furthermore, utilizing the Y and mitochondrial DNA test in conjunction with autosomal DNA matching at Family Tree DNA helps narrows possible relatives. The Advanced Matching feature allows you to see who you match on both the Y (or mitochondrial) DNA lines AND the autosomal test, in combination.

Mitochondrial DNA tests the matrilineal line only, as women pass their mitochondrial DNA to all of their children, but only females pass it on. Family Tree DNA provides matching and advanced combination matching/searching for mitochondrial DNA as well as Y DNA. Both genders of children carry their mother’s mitochondrial DNA. Unfortunately, mitochondrial DNA is more difficult to work with because of the surname changes in each generation, but you cannot be descended from a woman, or her direct matrilineal ancestors if you don’t substantially match her mitochondrial DNA.

Some vendors state that you receive mitochondrial DNA with your autosomal results, which is only partly accurate. At 23andMe, you receive a haplogroup but no detailed results and no matching. 23andMe does not test the entire mitochondria and therefore cannot provide either advanced haplogroup placement nor Y or mitochondrial DNA matching between testers.

For additional details on the Y and Mitochondrial DNA tests themselves and what you receive, please see the Genealogy – Y and Mitochondrial DNA section.

Adoption Summary

Adoptees should test with all 4 vendors plus Y and mitochondrial DNA testing.

  • Ancestry – due to their extensive data base size and trees
  • Family Tree DNA – due to their advanced tools, chromosome browser, Y and mitochondrial DNA tests (Ancestry and 23andMe participants can transfer autosomal raw data files and see matches for free, but advanced tools require either an unlock fee or a test on the Family Tree DNA platform)
  • 23andMe – no trees and many people don’t participate in sharing genetic information
  • MyHeritage – new kid on the block, working through what is hoped are startup issues
  • All adoptees should take the full mitochondrial sequence test.
  • Male adoptees should take the 111 marker Y DNA test, although you can start with 37 or 67 markers and upgrade later.
  • Y and mitochondrial tests are only available at Family Tree DNA.

Adoptee Vendor Feature Summary Chart

Family Tree DNA Ancestry 23andMe MyHeritage
Autosomal DNA – Males and Females
Matching Yes Yes Yes Yes – problems
Relationship Estimates* Yes – May be too close Yes – May be too distant Yes – Matches may not be sharing Yes –  problematic
International Reach Very strong Not strong but growing Not strong Small but subscriber base is European focused
Trees Yes Yes No Yes
Tree Quantity 54% have trees, 46% no tree (of my first 100 matches) 56% have trees, 44% no tree or private (of my first 100 matches) No trees ~50% don’t have trees or are private (cannot discern private tree without clicking on every tree)
Data Base Size Large Largest Large – but not all opt in to matching Very small
My # of Matches on 4-23-2017 2,421 23,750 1,809 but only 1,114 are sharing 75
Subscription Required No No for partial, Yes for full functionality including access to matches’ trees, minimal subscription for $49 by calling Ancestry No No for partial, Yes for full functionality
Other Relevant Tools New Ancestor Discoveries
Autosomal DNA Issues Many testers don’t have trees Many testers don’t have trees Matching opt-in is problematic, no trees at all Matching issues, small data base size is problematic, many testers don’t have trees
Contact Methodology E-mail address provided to matches Internal message system – known delivery issues Internal message system Internal message system
X Chromosome Matching Yes No Yes No
Y-DNA – Males Only
Y DNA STR Test Yes- 37, 67, and 111 markers No No No
Y Haplogroup Yes as part of STR test plus additional testing available No Yes, basic level but no additional testing available, outdated haplogroups No
Y Matching Yes No No No
Advanced Matching Between Y and Autosomal Yes No No No
Mitochondrial DNA- Males and Females
Test Yes, partial and full sequence No No No
Mitochondrial DNA Haplogroup Yes, included in test No Yes, basic but full haplogroup not available, haplogroup several versions behind No
Advanced Matching Between Mitochondrial and Autosomal Yes No No No

Genealogy – Cousin Matching and Ancestor Search/Verification

People who want to take a DNA test to find cousins, to learn more about their genealogy, to verify their genealogy research or to search for unknown ancestors and break down brick walls will be interested in various types of testing

Test Type Who Can Test
Y DNA – direct paternal line Males only
Mitochondrial DNA – direct matrilineal line Males and Females
Autosomal – all lines Males and Females

Let’s begin with autosomal DNA testing for genealogy which tests your DNA inherited from all ancestral lines.

Aside from ethnicity, autosomal DNA testing provides matches to other people who have tested. A combination of trees, meaning their genealogy, and their chromosome segments are used to identify (through trees) and verify (through DNA segments) common ancestor(s) and then to assign a particular DNA segment(s) to that ancestor or ancestral couple. This process, called triangulation, then allows you to assign specific segments to particular ancestors, through segment matching among multiple people. You then know that when another individual matches you and those other people on the same segment, that the DNA comes from that same lineage. Triangulation is the only autosomal methodology to confirm ancestors who are not close relatives, beyond the past 2-3 generations or so.

All three vendors provide matching, but the tools they include and their user interfaces are quite different. 

Genealogy – Autosomal –  Family Tree DNA

Family Tree DNA entered DNA testing years before any of the others, initially with Y and mitochondrial DNA testing.

Because of the diversity of their products, their website is somewhat busier, but they do a good job of providing areas on the tester’s personal landing page for each of the products and within each product, a link for each feature or function.

For example, the Family Finder test is Family Tree DNA’s autosomal test. Within that product, tools provided are:

  • Matching
  • Chromosome Browser
  • Linked Relationships
  • myOrigins
  • Ancient Origins
  • Matrix
  • Advanced Matching

Unique autosomal tools provided by Family Tree DNA are:

  • Linked Relationships that allows you to connect individuals that you match to their location in your tree, indicating the proper relationship. Phased Family Matching uses these relationships within your tree to indicate which side of your tree other matches originate from.
  • Phased Family Matching shows which side of your tree, maternal, paternal or both, someone descends from, based on phased DNA matching between you and linked relationship matches as distant as third cousins. This allows Family Tree DNA to tell you whether matches are paternal (blue icon), maternal (red icon) or both (purple icon) without a parent’s DNA. This is one of the best autosomal tools at Family Tree DNA, shown below.

  • In Common With and Not In Common With features allow you to sort your matches in common with another individual a number of ways, or matches not in common with that individual.
  • Filtered downloads provide the downloading of chromosome data for your filtered match list.
  • Stackable filters and searches – for example, you can select paternal matches and then search for a particular surname or ancestral surname within the paternal matches.
  • Common ethnicity matching through myOrigins allows you to see selected groups of individuals who match you and share common ethnicities.
  • Y and mtDNA locations of autosomal matches are provided on your ethnicity map through myOrigins.
  • Advanced matching tool includes Y, mtDNA and autosomal in various combinations. Also includes matches within projects where the tester is a member as well as by partial surname.
  • The matrix tool allows the tester to enter multiple people that they match in order to see if those individuals also match each other. The matrix tool is, in combination with the in-common-with tool and the chromosome browser is a form of pseudo triangulation, but does not indicate that the individuals match on the same segment.

  • Chromosome browser with the ability to select different segment match thresholds to display when comparing 5 or fewer individuals to your results.
  • Projects to join which provide group interaction and allow individuals to match only within the project, if desired.

To read more about how to utilize the various autosomal tools at Family Tree DNA, with examples, click here.

Genealogy – Autosomal – Ancestry

Ancestry only offers autosomal DNA testing to their customers, so their page is simple and straightforward.

Ancestry is the only testing vendor (other than MyHeritage who is not included in this section) to require a subscription for full functionality, although if you call the Ancestry support line, a minimal subscription is available for $49. You can see your matches without a subscription, but you cannot see your matches trees or utilize other functions, so you will not be able to tell how you connect to your matches. Many genealogists have Ancestry subscriptions, so this is minimally problematic for most people.

However, if you don’t realize you need a subscription initially, the required annual subscription raises the effective cost of the test quite substantially. If you let your subscription lapse, you no longer have access to all DNA features. The cost of testing with Ancestry is the cost of the test plus the cost of a subscription if you aren’t already a subscriber.

This chart, from the Ancestry support center, provides details on which features are included for free and which are only available with a subscription.

Unique tools provided by Ancestry include:

  • Shared Ancestor Hints (green leaves) which indicate a match with whom you share a common ancestor in your tree connected to your DNA, allowing you to display the path of you and your match to the common ancestor. In order to take advantage of this feature, testers must link their tree to their DNA test. Otherwise, Ancestry can’t do tree matching.  As far as I’m concerned, this is the single most useful DNA tool at Ancestry. Subscription required.

  • DNA Circles, example below, are created when several people whose DNA matches also share a common ancestor. Subscription required.

  • New Ancestor Discoveries (NADs), which are similar to Circles, but are formed when you match people descended from a common ancestor, but don’t have that ancestor in your tree. The majority of the time, these NADs are incorrect and are, when dissected and the source can be determined, found to be something like the spouse of a sibling of your ancestor. I do not view NADs as a benefit, more like a wild goose chase, but for some people these could be useful so long as the individual understands that these are NOT definitely ancestors and only hints for research. Subscription required.
  • Ancestry uses a proprietary algorithm called Timber to strip DNA from you and your matches that they consider to be “too matchy,” with the idea that those segments are identical by population, meaning likely to be found in large numbers within a population group – making them meaningless for genealogy. The problem is that Timber results in the removal of valid segments, especially in endogamous groups like Acadian families. This function is unique to Ancestry, but many genealogists (me included) don’t consider Timber a benefit.
  • Genetic Communities shows you groups of individuals with whom your DNA clusters. The trees of cluster members are then examined by Ancestry to determine connections from which Genetic Communities are formed. You can filter your DNA match results by Genetic Community.

Genealogy – Autosomal – 23and Me

Unfortunately, the 23andMe website is not straightforward or intuitive. They have spent the majority of the past two years transitioning to a “New Experience” which has resulted in additional confusion and complications when matching between people on multiple different platforms. You can take a spin through the New Experience by clicking here.

23andMe requires people to opt-in to sharing, even after they have selected to participate in Ancestry Services (genealogy) testing, have opted-in previously and chosen to view their DNA Relatives. Users on the “New Experience” can then either share chromosome data and results with each other individually, meaning on a one by one basis, or globally by a one-time opt-in to “open sharing” with matches. If a user does not opt-in to both DNA Relatives and open sharing, sharing requests must be made individually to each match, and they must opt-in to share with each individual user. This complexity and confusion results in an approximate sharing rate of between 50 and 60%. One individual who religiously works their matches by requesting sharing now has a share rate of about 80% of their matches in the data base who HAVE initially selected to participate in DNA Relatives. You can read more about the 23andMe experience at this link.

Various genetic genealogy reports and tools are scattered between the Reports and Tools tabs, and within those, buried in non-intuitive locations. If you are going to utilize 23andMe for matching and genealogy, in addition to the above link, I recommend Kitty Cooper’s blogs about the new DNA Relatives here and on triangulation here. Print the articles, and use them as a guide while navigating the 23andMe site.

Note that some screens (the Tools, DNA Relatives, then DNA tab) on the site do not display/work correctly utilizing Internet Explorer, but do with Edge or other browsers.

The one genealogy feature unique to 23andMe is:

  • Triangulation at 23andMe allows you to select a specific match to compare your DNA against. Several pieces of information will be displayed, the last of which, scrolling to the bottom, is a list of your common relatives with the person you selected.

In the example below, I’ve selected to see the matches I match in common with known family member, Stacy Den (surnames have been obscured for privacy reasons.)  Please note that the Roberta V4 Estes kit is a second test that I took for comparison purposes when the new V4 version of 23andMe was released.  Just ignore that match, because, of course I match myself as a twin.

If an individual does not match both you and your selected match, they will not appear on this list.

In the “relatives in common” section, each person is listed with a “shared DNA” column. For a person to be shown on this “in common” list, you obviously do share DNA with these individuals and they also share with your match, but the “shared DNA” column goes one step further. This column indicates whether or not you and your match both share a common DNA segment with the “in common” person.

I know this is confusing, so I’ve created this chart to illustrate what will appear in the “Shared DNA” column of the individuals showing on the list of matches, above, shared between me and Stacy Den.

Clicking on “Share to see” sends Sarah a sharing request for her to allow you to see her segment matches.

Let’s look at an example with “yes” in the Shared DNA column.

Clicking on the “Yes” in the Shared DNA column of Debbie takes us to the chromosome browser which shows both your selected match, Stacy in my case, and Debbie, the person whose “yes” you clicked.

All three people, meaning me, Stacy and Debbie share a common DNA segment, shown below on chromosome 17.

What 23andMe does NOT say is that these people. Stacy and Debbie, also match each other, in addition to matching me, which means all three of us triangulate.

Because I manage Stacy’s kit at 23andMe, I can check to see if Debbie is on Stacy’s match list, and indeed, Debbie is on Stacy’s match list and Stacy does match both Debbie and me on chromosome 17 in exactly the same location shown above, proving unquestionably that the three of us all match each other and therefore triangulate on this segment. In our case, it’s easy to identify our common relative whose DNA all 3 of us share.

Genealogy – Autosomal Summary

While all 3 vendors offer matching, their interfaces and tools vary widely.

I would suggest that Ancestry is the least sophisticated and has worked hard to make their tools easy for the novice working with genetic genealogy. Their green leaf DNA+Tree Matching is their best feature, easy to use and important for the novice and experienced genealogist alike.  Now, if they just had that chromosome browser so we could see how we match those people.

Ancestry’s Circles, while a nice feature, encourage testers to believe that their DNA or relationship is confirmed by finding themselves in a Circle, which is not the case.

Circles can be formed as the result of misinformation in numerous trees. For example, if I were to inaccurately list Smith as the surname for one of my ancestor’s wives, I would find myself in a Circle for Barbara Smith, when in fact, there is absolutely no evidence whatsoever that her surname is Smith. Yet, people think that Barbara Smith is confirmed due to a Circle having been formed and finding themselves in Barbara Smith’s Circle. Copying incorrect trees equals the formation of incorrect Circles.

It’s also possible that I’m matching people on multiple lines and my DNA match to the people in any given Circle is through another common ancestor entirely.

A serious genealogist will test minimally at Ancestry and at Family Tree DNA, who provides a chromosome browser and other tools necessary to confirm relationships and shared DNA segments.

Family Tree DNA is more sophisticated, so consequently more complex to use.  They provide matching plus numerous other tools. The website and matching is certainly friendly for the novice, but to benefit fully, some experience or additional education is beneficial, not unlike traditional genealogy research itself. This is true not just for Family Tree DNA, but GedMatch and 23andMe who all three utilize chromosome browsers.

The user will want to understand what a chromosome browser is indicating about matching DNA segments, so some level of education makes life a lot easier. Fortunately, understanding chromosome browser matching is not complex. You can read an article about Match Groups and Triangulation here. I also have an entire series of Concepts articles, Family Tree DNA offers a webinar library, their Learning Center and other educational resources are available as well.

Family Tree DNA is the only vendor to provide Phased Family Matches, meaning that by connecting known relatives who have DNA tested to your tree, Family Tree DNA can then identify additional matches as maternal, paternal or both. This, in combination with pseudo-phasing are very powerful matching tools.

23andMe is the least friendly of the three companies, with several genetic genealogy unfriendly restrictions relative to matching, opt-ins, match limits and such. They have experienced problem after problem for years relative to genetic genealogy, which has always been a second-class citizen compared to their medical research, and not a priority.

23andMe has chosen to implement a business model where their customers must opt-in to share segment information with other individuals, either one by one or by opting into open sharing. Based on my match list, roughly 60% of my actual DNA matches have opted in to sharing.

Their customer base includes fewer serious genealogists and their customers often are not interested in genealogy at all.

Having said that, 23andMe is the only one of the three that provides actual triangulated matches for users on the New Experience and who have opted into sharing.

If I were entering the genetic genealogy testing space today, I would test my autosomal DNA at Ancestry and at Family Tree DNA, but I would probably not test at 23andMe. I would test both my Y DNA (if a male) and mitochondrial at Family Tree DNA.

Thank you to Kitty Cooper for assistance with parent/child matching and triangulation at 23andMe.

Genealogy Autosomal Vendor Feature Summary Chart

Family Tree DNA Ancestry 23andMe
Matching Yes Yes Yes – each person has to opt in for open sharing or authorize sharing individually, many don’t
Estimated Relationships Yes Yes Yes
Chromosome Browser Yes No – Large Issue Yes
Chromosome Browser Threshold Adjustment Yes No Chromosome Browser No
X Chromosome Matching Yes No Yes
Trees Yes Yes – subscription required so see matches’ trees No
Ability to upload Gedcom file Yes Yes No
Ability to search trees Yes Yes No
Subscription in addition to DNA test price No No for partial, Yes for full functionality, minimal subscription for $49 by calling Ancestry No
DNA + Ancestor in Tree Matches No Yes – Leaf Hints – subscription required – Best Feature No
Phased Parental Side Matching Yes – Best Feature No No
Parent Match Indicator Yes No Yes
Sort or Group by Parent Match Yes Yes Yes
In Common With Tool Yes Yes Yes
Not In Common With Tool Yes No No
Triangulated Matches No – pseudo with ICW, browser and matrix No Yes – Best Feature
Common Surnames Yes Yes – subscription required No
Ability to Link DNA Matches on Tree Yes No No
Matrix to show match grid between multiple matches Yes No No
Match Filter Tools Yes Minimal Some
Advanced Matching Tool Yes No No
Multiple Test Matching Tool Yes No multiple tests No multiple tests
Ethnicity Matching Yes No Yes
Projects Yes No No
Maximum # of Matches Restricted No No Yes – 2000 unless you are communicating with the individuals, then they are not removed from your match list
All Customers Participate Yes Yes, unless they don’t have a subscription No – between 50-60% opt-in
Accepts Transfers from Other Testing Companies Yes No No
Free Features with Transfer Matching, ICW, Matrix, Advanced Matching No transfers No transfers
Transfer Features Requiring Unlock $ Chromosome Browser, Ethnicity, Ancient Origins, Linked Relationships, Parentally Phased Matches No Transfers No transfers
Archives DNA for Later Testing Yes, 25 years No, no additional tests available No, no additional tests available
Additional Tool DNA Circles – subscription required
Additional Tool New Ancestor Discoveries – subscription required
Y DNA Not included in autosomal test but is additional test, detailed results including matching No Haplogroup only
Mitochondrial DNA Not included in autosomal test but is additional test, detailed results including matching No Haplogroup only
Advanced Testing Available Yes No No
Website Intuitive Yes, given their many tools Yes, very simple No
Data Base Size Large Largest Large but many do not test for genealogy, only test for health
Strengths Many tools, multiple types of tests, phased matching without parent DNA + Tree matching, size of data base Triangulation
Challenges Website episodically times out No chromosome browser or advanced tools Sharing is difficult to understand and many don’t, website is far from intuitive

 

Genealogy – Y and Mitochondrial DNA

Two indispensable tools for genetic genealogy that are often overlooked are Y and mitochondrial DNA.

The inheritance path for Y DNA is shown by the blue squares and the inheritance path for mitochondrial DNA is shown by the red circles for the male and female siblings shown at the bottom of the chart.

Y-DNA Testing for Males

Y DNA is inherited by males only, from their father. The Y chromosome makes males male. Women instead inherit an X chromosome from their father, which makes them female. Because the Y chromosome is not admixed with the DNA of the mother, the same Y chromosome has been passed down through time immemorial.

Given that the Y chromosome follows the typical surname path, Y DNA testing is very useful for confirming surname lineage to an expected direct paternal ancestor. In other words, an Estes male today should match, with perhaps a few mutations, to other descendants of Abraham Estes who was born in 1647 in Kent, England and immigrated to the colony of Virginia.

Furthermore, that same Y chromosome can look far back in time, thousands of years, to tell us where that English group of Estes men originated, before the advent of surnames and before the migration to England from continental Europe. I wrote about the Estes Y DNA here, so you can see an example of how Y DNA testing can be used.

Y DNA testing for matching and haplogroup identification, which indicates where in the world your ancestors were living within the past few hundred to few thousand years, is only available from Family Tree DNA. Testing can be purchased for either 37, 67 or 111 markers, with the higher marker numbers providing more granularity and specificity in matching.

Family Tree DNA provides three types of Y DNA tests.

  • STR (short tandem repeat) testing is the traditional Y DNA testing for males to match to each other in a genealogically relevant timeframe. These tests can be ordered in panels of 37, 67 or 111 markers and lower levels can be upgraded to higher levels at a later date. An accurate base haplogroup prediction is made from STR markers.
  • SNP (single nucleotide polymorphism) testing is a different type of testing that tests single locations for mutations in order to confirm and further refine haplogroups. Think of a haplogroup as a type of genetic clan, meaning that haplogroups are used to track migration of humans through time and geography, and are what is utilized to determine African, European, Asian or Native heritage in the direct paternal line. SNP tests are optional and can be ordered one at a time, in groups called panels for a particular haplogroup or a comprehensive research level Y DNA test called the Big Y can be ordered after STR testing.
  • The Big Y test is a research level test that scans the entire Y chromosome to determine the most refined haplogroup possible and to report any previously unknown mutations (SNPs) that may define further branches of the Y DNA tree. This is the technique used to expand the Y haplotree.

You can read more about haplogroups here and about the difference between STR markers and SNPs here, here and here.

Customers receive the following features and tools when they purchase a Y DNA test at Family Tree DNA or the Ancestry Services test at 23andMe. The 23andMe Y DNA information is included in their Ancestry Services test. The Family Tree DNA Y DNA information requires specific tests and is not included in the Family Finder test. You can click here to read about the difference in the technology between Y DNA testing at Family Tree DNA and at 23andMe. Ancestry is not included in this comparison because they provide no Y DNA related information.

Y DNA Vendor Feature Summary Chart

Family Tree DNA 23andMe
Varying levels of STR panel marker testing Yes, in panels of 37, 67 and 111 markers No
Test panel (STR) marker results Yes Not tested
Haplogroup assignment Yes – accurate estimate with STR panels, deeper testing available Yes –base haplogroup by scan – haplogroup designations are significantly out of date, no further testing available
SNP testing to further define haplogroup Yes – can purchase individual SNPs, by SNP panels or Big Y test No
Matching to other participants Yes No
Trees available for your matches Yes No
E-mail of matches provided Yes No
Calculator tool to estimate probability of generational distance between you and a match Yes No
Earliest known ancestor information Yes No
Projects Surname, haplogroup and geographic projects No
Ability to search Y matches Yes No Y matching
Ability to search matches within projects Yes No projects
Ability to search matches by partial surname Yes No
Haplotree and customer result location on tree Yes, detailed with every branch Yes, less detailed, subset
Terminal SNP used to determine haplogroup Yes Yes, small subset available
Haplogroup Map Migration map Heat map
Ancestral Origins – summary by ancestral location of others you match, by test level Yes No
Haplogroup Origins – match ancestral location summary by haplogroup, by test level Yes No
SNP map showing worldwide locations of any selected SNP Yes No
Matches map showing mapped locations of your matches most distant ancestor in the paternal line, by test panel Yes No
Big Y – full scan of Y chromosome for known and previously unknown mutations (SNPs) Yes No
Big Y matching Yes No
Big Y matching known SNPs Yes No
Big Y matching novel variants (unknown or yet unnamed SNPs) Yes No
Filter Big Y matches Yes No
Big Y results Yes No
Advanced matching for multiple test types Yes No
DNA is archived so additional tests or upgrades can be ordered at a later date Yes, 25 years No

Mitochondrial DNA Testing for Everyone

Mitochondrial DNA is contributed to both genders of children by mothers, but only the females pass it on. Like the Y chromosome, mitochondrial DNA is not admixed with the DNA of the other parent. Therefore, anyone can test for the mitochondrial DNA of their matrilineal line, meaning their mother’s mother’s mother’s lineage.

Matching can identify family lines as well as ancient lineage.

You receive the following features and tools when you purchase a mitochondrial DNA test from Family Tree DNA or the Ancestry Services test from 23andMe. The Family Tree DNA mitochondrial DNA information requires specific tests and is not included in the Family Finder test. The 23andMe mitochondrial information is provided with the Ancestry Services test. Ancestry is omitted from this comparison because they do not provide any mitochondrial information.

Mitochondrial DNA Vendor Feature Summary Chart

Family Tree DNA 23andMe
Varying levels of testing Yes, mtPlus and Full Sequence No
Test panel marker results Yes, in two formats, CRS and RSRS No
Rare mutations, missing and extra mutations, insertions and deletions reported Yes No
Haplogroup assignment Yes, most current version, Build 17 Yes, partial and out of date version
Matching to other participants Yes No
Trees of matches available to view Yes No
E-mail address provided to matches Yes No
Earliest known ancestor information Yes No
Projects Surname, haplogroup and geographic available No
Ability to search matches Yes No
Ability to search matches within project Yes No projects
Ability to search match by partial surname Yes No
Haplotree and customer location on tree No Yes
Mutations used to determine haplogroup provided Yes No
Haplogroup Map Migration map Heat map
Ancestral Origins – summary by ancestral location of others you match, by test level Yes No
Haplogroup Origins –match ancestral location summary by haplogroup Yes No
Matches map showing mapped locations of your matches most distant ancestor in the maternal line, by test level Yes No
Advanced matching for multiple test types Yes No
DNA is archived so additional tests or upgrades can be ordered at a later date Yes, 25 years No

 

Overall Genealogy Summary

Serious genealogists should test with at least two of the three major vendors, being Family Tree DNA and Ancestry, with 23andMe coming in as a distant third.

No genetic genealogy testing regimen is complete without Y and mitochondrial DNA for as many ancestral lines as you can find to test. You don’t know what you don’t know, and you’ll never know if you don’t test.

Unfortunately, many people, especially new testers, don’t know Y and mitochondrial DNA testing for genetic genealogy exists, or how it can help their genealogy research, which is extremely ironic since these were the first tests available, back in 2000.

You can read about finding Y and mitochondrial information for various family lines and ancestors and how to assemble a DNA Pedigree Chart here.

You can also take a look at my 52 Ancestors series, where I write about an ancestor every week. Each article includes some aspect of DNA testing and knowledge gained by a test or tests, DNA tool, or comparison. The DNA aspect of these articles focuses on how to use DNA as a tool to discover more about your ancestors.

Testing for Medical/Health or Traits

The DTC market also includes health and medical testing, although it’s not nearly as popular as genetic genealogy.

Health/medical testing is offered by 23andMe, who also offers autosomal DNA testing for genealogy.

Some people do want to know if they have genetic predispositions to medical conditions, and some do not. Some want to know if they have certain traits that aren’t genealogically relevant, but might be interesting – such as whether they carry the Warrior gene or if they have an alcohol flush reaction.

23andMe was the first company to dip their toes into the water of Direct to Consumer medical information, although they called it “health,” not medicine, at that time. Regardless of the terminology, information regarding Parkinson’s and Alzheimer’s, for example, were provided for customers. 23andMe attempted to take the raw data and provide the consumer with something approaching a middle of the road analysis, because sometimes the actual studies provide conflicting information that might not be readily understood by consumers.

The FDA took issue with 23andMe back in November of 2013 when they ordered 23andMe to discontinue the “health” aspect of their testing after 23andMe ignored several deadlines. In October 2015, 23andMe obtained permission to provide customers with some information, such as carrier status, for 36 genetic disorders.

Since that time, 23andMe has divided their product into two separate tests, with two separate prices. The genealogy only test called Ancestry Service can be purchased separately for $99, or the combined Health + Ancestry Service for $199.

If you are interested in seeing what the Health + Ancestry test provides, you can click here to view additional information.

However, there is a much easier and less expensive solution.

If you have taken the autosomal test from 23andMe, Ancestry or Family Tree DNA, you can download your raw data file from the vendor and upload to Promethease to obtain a much more in-depth report than is provided by 23andMe, and much less expensively – just $5.

I reviewed the Promethease service here. I found the Promethease reports to be very informative and I like the fact that they provide information, both positive and negative for each SNP (DNA location) reported. Promethease avoids FDA problems by not providing any interpretation or analysis, simply the data and references extracted from SNPedia for you to review.

I would be remiss if I didn’t mention that you should be sure you really want to know before you delve into medical testing. Some mutations are simply indications that you could develop a condition that you will never develop or that is not serious. Other mutations are not so benign. Promethease provides this candid page before you upload your data.

Different files from different vendors provide different results at Promethease, because those vendors test different SNP locations in your DNA. At the Promethease webpage, you can view examples.

Traits

Traits fall someplace between genealogy and health. When you take the Health + Ancestry test at 23andMe, you do receive information about various traits, as follows:

Of course, you’ll probably already know if you have several of these traits by just taking a look in the mirror, or in the case of male back hair, by asking your wife.

At Family Tree DNA, existing customers can order tests for Factoids (by clicking on the upgrade button), noted as curiosity tests for gene variants.

Family Tree DNA provides what I feel is a great summary and explanation of what the Factoids are testing on their order page:

“Factoids” are based on studies – some of which may be controversial – and results are not intended to diagnose disease or medical conditions, and do not serve the purpose of medical advice. They are offered exclusively for curiosity purposes, i.e. to see how your result compared with what the scientific papers say. Other genetic and environmental variables may also impact these same physiological characteristics. They are merely a conversational piece, or a “cocktail party” test, as we like to call it.”

Test Price Description
Alcohol Flush Reaction $19 A condition in which the body cannot break down ingested alcohol completely. Flushing, after consuming one or two alcoholic beverages, includes a range of symptoms: nausea, headaches, light-headedness, an increased pulse, occasional extreme drowsiness, and occasional skin swelling and itchiness. These unpleasant side effects often prevent further drinking that may lead to further inebriation, but the symptoms can lead to mistaken assumption that the people affected are more easily inebriated than others.
Avoidance of Errors $29 We are often angry at ourselves because we are unable to learn from certain experiences. Numerous times we have made the wrong decision and its consequences were unfavorable. But the cause does not lie only in our thinking. A mutation in a specific gene can also be responsible, because it can cause a smaller number of dopamine receptors. They are responsible for remembering our wrong choices, which in turn enables us to make better decisions when we encounter a similar situation.
Back Pain $39 Lumbar disc disease is the drying out of the spongy interior matrix of an intervertebral disc in the spine. Many physicians and patients use the term lumbar disc disease to encompass several different causes of back pain or sciatica. A study of Asian patients with lumbar disc disease showed that a mutation in the CILP gene increases the risk of back pain.
Bitter Taste Perception $29 There are several genes that are responsible for bitter taste perception – we test 3 of them. Different variations of this gene affect ability to detect bitter compounds. About 25% of people lack ability to detect these compounds due to gene mutations. Are you like them? Maybe you don’t like broccoli, because it tastes too bitter?
Caffeine Metabolism $19 According to the results of a case-control study reported in the March 8, 2006 issue of JAMA, coffee is the most widely consumed stimulant in the world, and caffeine consumption has been associated with increased risk for non-fatal myocardial infarction. Caffeine is primarily metabolized by the cytochrome P450 1A2 in the liver, accounting for 95% of metabolism. Carriers of the gene variant *1F allele are slow caffeine metabolizers, whereas individuals homozygous for the *1A/*1A genotype are rapid caffeine metabolizers.
Earwax Type $19 Whether your earwax is wet or dry is determined by a mutation in a single gene, which scientists have discovered. Wet earwax is believed to have uses in insect trapping, self-cleaning and prevention of dryness in the external auditory canal of the ear. It also produces an odor and causes sweating, which may play a role as a pheromone.
Freckling $19 Freckles can be found on anyone no matter what the background. However, having freckles is genetic and is related to the presence of the dominant melanocortin-1 receptor MC1R gene variant.
Longevity $49 Researchers at Harvard Medical School and UC Davis have discovered a few genes that extend lifespan, suggesting that the whole family of SIR2 genes is involved in controlling lifespan. The findings were reported July 28, 2005 in the advance online edition of Science.
Male Pattern Baldness $19 Researchers at McGill University, King’s College London and GlaxoSmithKline Inc. have identified two genetic variants in Caucasians that together produce an astounding sevenfold increase of the risk of male pattern baldness. Their results were published in the October 12, 2008 issue of the Journal of Nature Genetics.
Monoamine Oxidase A (Warrior Gene) $49.50 The Warrior Gene is a variant of the gene MAO-A on the X chromosome. Recent studies have linked the Warrior Gene to increased risk-taking and aggressive behavior. Whether in sports, business, or other activities, scientists found that individuals with the Warrior Gene variant were more likely to be combative than those with the normal MAO-A gene. However, human behavior is complex and influenced by many factors, including genetics and our environment. Individuals with the Warrior Gene are not necessarily more aggressive, but according to scientific studies, are more likely to be aggressive than those without the Warrior Gene variant. This test is available for both men and women, however, there is limited research about the Warrior Gene variant amongst females. Additional details about the Warrior Gene genetic variant of MAO-A can be found in Sabol et al, 1998.
Muscle Performance $29 A team of researchers, led by scientists at Dartmouth Medical School and Dartmouth College, have identified and tested a gene that dramatically alters both muscle metabolism and performance. The researchers say that this finding could someday lead to treatment of muscle diseases, including helping the elderly who suffer from muscle deterioration and improving muscle performance in endurance athletes.
Nicotine Dependence $19 In 2008, University of Virginia Health System researchers have identified a gene associated with nicotine dependence in both Europeans and African Americans.

Many people are interested in the Warrior Gene, which I wrote about here.

At Promethease, traits are simply included with the rest of the conditions known to be associated with certain SNPs, such as baldness, for example, but I haven’t done a comparison to see which traits are included.

 

Additional Vendor Information to Consider

Before making your final decision about which test or tests to purchase, there are a few additional factors you may want to consider.

As mentioned before, Ancestry requires a subscription in addition to the cost of the DNA test for the DNA test to be fully functional.

One of the biggest issues, in my opinion, is that both 23andMe and Ancestry sell customer’s anonymized DNA information to unknown others. Every customer authorizes the sale of their information when they purchase or activate a kit – even though very few people actually take the time to read the Terms and Conditions, Privacy statements and Security documents, including any and all links. This means most people don’t realize they are authorizing the sale of their DNA.

At both 23andMe and Ancestry, you can ALSO opt in for additional non-anonymized research or sale of your DNA, which you can later opt out of. However, you cannot opt out of the lower level sale of your anonymized DNA without removing your results from the data base and asking for your sample to be destroyed. They do tell you this, but it’s very buried in the fine print at both companies. You can read more here.

Family Tree DNA does not sell your DNA or information.

All vendors can change their terms and conditions at any time. Consumers should always thoroughly read the terms and conditions including anything having to do with privacy for any product they purchase, but especially as it relates to DNA testing.

Family Tree DNA archives your DNA for later testing, which has proven extremely beneficial when a family member has passed away and a new test is subsequently introduced or the family wants to upgrade a current test.  Had my mother’s DNA not been archived at Family Tree DNA, I would not have Family Finder results for her today – something I thank Mother and Family Tree DNA for every single day.

Family Tree DNA also accepts transfer files from 23andMe, Ancestry and very shortly, MyHeritage – although some versions work better than others. For details on which companies accept which file versions, from which vendors, and why, please read Autosomal DNA Transfers – Which Companies Accept Which Tests?

If you tested on a compatible version of the 23andMe Test (V3 between December 2010 and November 2013) or the Ancestry V1 (before May 2016) you may want to transfer your raw data file to Family Tree DNA for free and pay only $19 for full functionality, as opposed to taking the Family Finder test. Family Tree DNA does accept later versions of files from 23andMe and Ancestry, but you will receive more matches if you test on the same chip platform that Family Tree DNA utilizes instead of doing a transfer.

Additional Vendor Considerations Summary Chart

Family Tree DNA Ancestry 23andMe
Subscription required in addition to cost of DNA test No Yes for full functionality, partial functionality is included without subscription, minimum subscription is $49 by calling Ancestry No
Customer Support Good and available Available, nice but often not knowledgeable about DNA Poor
Sells customer DNA information No Yes Yes
DNA raw data file available to download Yes Yes Yes
DNA matches file available to download including match info and chromosome match locations Yes No Yes
Customers genealogically focused Yes Yes Many No
Accepts DNA raw data transfer files from other companies Yes, most, see article for specifics No No
DNA archived for later testing Yes, 25 years No No
Beneficiary provision available Yes No No

 

Which Test is Best For You?

I hope you now know the answer as to which DNA test is best for you – or maybe it’s multiple tests for you and other family members too!

DNA testing holds so much promise for genealogy. I hesitate to call DNA testing a miracle tool, but it often is when there are no records. DNA testing works best in conjunction with traditional genealogical research.

There are a lot of tests and options.  The more tests you take, the more people you match. Some people test at multiple vendors or upload their DNA to third party sites like GedMatch, but most don’t. In order to make sure you reach those matches, which may be the match you desperately need, you’ll have to test at the vendor where they tested. Otherwise, they are lost to you. That means, of course, that eventually, if you’re a serious genealogist, you’ll be testing at all 3 vendors.  Don’t forget about Y and mitochondrial tests at Family Tree DNA.

Recruit family members to test and reach out to your matches.  The more you share and learn – the more is revealed about your ancestors. You are, after all, the unique individual that resulted from the combination of all of them!

Update: Vendor prices updated June 22, 2017.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

Concepts – Percentage of Ancestors’ DNA

A very common question is, “How much DNA of an ancestor do I carry and how does that affect my ethnicity results?”

This question is particularly relevant for people who are seeking evidence of a particular ethnicity of an ancestor several generations back in time. I see this issue raise its head consistently when people take an ethnicity test and expect that their “full blood” Native American great-great-grandmother will show up in their results.

Let’s take a look at how DNA inheritance works – and why they might – or might not find the Native DNA they seek, assuming that great-great-grandma actually was Native.

Inheritance

Every child inherits exactly 50% of their autosomal DNA from each parent (except for the X chromosome in males.) However, and this is a really important however, the child does NOT inherit exactly half of the DNA of each ancestor who lived before the parents. How can this be, you ask?

Let’s step through this logically.

The number of ancestors you have doubles in each generation, going back in time.

This chart provides a summary of how many ancestors you have in each generation, an approximate year they were born using a 25 year generation and a 30 year generation, respectively, and how much of their DNA, on average, you could expect to carry, today. You’ll notice that by the time you’re in the 7th generation, you can be expected, on average, to carry 0.78% meaning less than 1% of that GGGGG-grandparent’s DNA.

Looking at the chart, you can see that you reach the 1% level at about the 6th generation with an ancestor probably born in the late 1700s or early 1800s.

It’s also worth noting here that generations can be counted differently. In some instances, you are counted as generation one, so your GGGGG-grandparent would be generation 8.

In general, DNA showing ethnicity below about 5% is viewed as somewhat questionable and below 2% is often considered to be “noise.” Clearly, that isn’t always the case, especially if you are dealing with continental level breakdowns, as opposed to within Europe, for example. Intra-continental (regional) ethnicity breakdowns are particularly difficult and unreliable, but continental level differences are easier to discern and are considered to be more reliable, comparatively.

If you want to learn more about how ethnicity calculations are derived and what they mean, please read the article Ethnicity Testing – A Conundrum.

On Average May Not Mean You

On average, each child receives half of the DNA of each ancestor from their parent.

The words “on average” are crucial to this discussion, because the average assumes that in fact each generation between your GGGGG-grandmother and you inherited exactly half of the DNA in each generation from their parent that was contributed by that GGGGG-grandmother.

Unfortunately, while averages are all that we have to work with, that’s not always how ancestral DNA is passed in each generation.

Let’s say that your GGGGG-grandmother was indeed full Native, meaning no admixture at all.

You can click to enlarge images.

Using the chart above, you can see that your GGGGG-grandmother was full native on all 20 “pieces” or segments of DNA used for this illustration. Those segments are colored red. The other 10 segments, with no color, were contributed by the father.

Let’s say she married a person who was not Native, and in every generation since, there were no additional Native ancestors.

Her child, generation 6, inherited exactly 50% of her DNA, shown in red – meaning 10 segments..

Generation 5, her grandchild, inherited exactly half of her DNA that was carried by the parent, shown in red – meaning 5 segments..

However, in the next generation, generation 4, that child inherited more than half of the Native DNA from their parent. They inherited half of their parent’s DNA, but the half that was randomly received included 3 Native segments out of a possible 5 Native segments that the parent carried.

In generation 3, that child inherited 2 of the possible 3 segments that their parent carried.

In generation 2, that person inherited all of the Native DNA that their parent carried.

In generation 1, your parent inherited half of the DNA that their parent carried, meaning one of 2 segments of Native DNA carried by your grandparent.

And you will either receive all of that one segment, part of that one segment, or none of that one segment.

In the case of our example, you did not inherit that segment, which is why you show no Native admixture, even though your GGGGG-grandmother was indeed fully Native..

Of course, even if you had inherited that Native segment, and that segment isn’t something the population reference models recognize as “Native,” you still won’t show as carrying any Native at all. It could also be that if you had inherited the red segment, it would have been too small and been interpreted as noise.

The “Received” column at the right shows how much of the ancestral DNA the current generation received from their parent.

The “% of Original” column shows how the percentage of GGGGG-grandmother’s DNA is reduced in each generation.

The “Expected” column shows how much DNA, “on average” we would expect to see in each generation, as compared to the “% of Original” which is how much they actually carry.

I intentionally made the chart, above, reflect a scenario close to what we could expect, on average. However, it’s certainly within the realm of possibility to see something like the following scenario, as well.

In the second example, above, neither you nor your parent or grandparent inherited any of the Native segments.

It’s also possible to see a third example, below, where 4 generations in a row, including you, inherited the full amount of Native DNA segments carried by the GG-grandparent.

Testing Other Relatives

Every child of every couple inherits different DNA from their parents. The 50% of their parents’ DNA that they inherit is not all the same. The three example charts above could easily represent three children of the GG-Grandparent and their descendants.

The pedigree chart below shows the three different examples, above.  The great-great-grandparent in the 4th generation who inherited 3 Native DNA segments is shown first, then the inheritance of the Native segments through all 3 children to the current generation.

Therefore, you may not have inherited the red segment of GGGGG-grandmother’s Native DNA, but your sibling might, or vice versa. As you can see in the chart above, one of your third cousins received 3 native segments from GGGGG-grandmother. but your other third cousin received none.

You can see why people are always encouraged to test their parents and grandparents as well as siblings. You never know where your ancestor’s DNA will turn up, and each person will carry a different amount, and different segments of DNA from your common ancestors.

In other words, your great-aunt and great-uncle’s DNA is every bit as important to you as your own grandparent’s DNA – so test everyone in older generations while you can, and their children if they are no longer available.

Back to Great-Great-Grandma

Going back to great-great-grandma and her Native heritage. You may not show Native ethnicity when you expected to see Native, but you may have other resources and recourses. Don’t give up!

Reason Resources and Comments
She really wasn’t Native. Genealogical research will help and mitochondrial DNA testing of an appropriate descendant will point the way to her true ethnic heritage, at least on her mother’s side.
She was Native, but the ethnicity test doesn’t show that I am. Test relatives and find someone descended from her through all females to take a mitochondrial test. The mitochondrial test will answer the question for her matrilineal line unquestionably.
She was partly, but not fully Native. This would mean that she had less Native DNA than you thought, which would mean the percentage coming to you is lower on average than anticipated. Mitochondrial DNA testing someone descended from her through all females to the current generation, which can be male, would reveal whether her mother was Native from her mother’s line.
She was Native, but several generations back in time. You or your siblings may show small percentages of Native or other locations considered to be a component of Native admixture in the absence of any other logical explanation for their presence, such as Siberian or Eastern Asian.

Using Y and Mitochondrial DNA Testing to Supplement Ethnicity Testing

When in doubt about ethnicity results, find an appropriately descended person to take a Y DNA test (males only, for direct paternal lineage) or a mitochondrial DNA test, for direct matrilineal results. These tests will yield haplogroup information and haplogroups are associated with specific world regions and ethnicities, providing a more definitive answer regarding the heritage of that specific line.

Y DNA reflects the direct male line, shown in blue above, and mitochondrial DNA reflects the direct matrilineal line, shown in red. Only males carry Y DNA, but both genders carry mitochondrial DNA.

For a short article about the different kinds of DNA and how they can help genealogists, please read 4 Kinds of DNA for Genetic Genealogy.

Ethnicity testing is available from any of the 3 major vendors, meaning Family Tree DNA, Ancestry or 23andMe. Base haplogroups are provided with 23andMe results, but detailed testing for Y and mitochondrial DNA is only available from Family Tree DNA.

To read about the difference between the two types of testing utilized for deriving haplogroups between 23andMe and Family Tree DNA, please read Haplogroup Comparisons between Family Tree DNA and 23andMe.

For more information on haplogroups, please read What is a Haplogroup?

For a discussion about testing family members, please read Concepts – Why DNA Testing the Oldest Family Members is Critically Important.

If you’d like to read a more detailed explanation of how inheritance works, please read Concepts – How Your Autosomal DNA Identifies Your Ancestors.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

Concepts – “Who To Test?” Series

I often receive questions about who to test to obtain (discover) the Y or mitochondrial DNA of a particular ancestor in one’s tree. The question often arises when people are attempting to find either Y or mitochondrial DNA to confirm that an ancestor descends from or belongs to a particular population.

For example, “My great-great-grandmother was supposed to be Cherokee.  How can I tell if she was?”

The answer would be that if she was Cherokee on her mother’s direct maternal side, testing the mitochondrial DNA of specific descendants would yield the answer.

Regardless of origins, the concept and techniques apply to everyone. People of Native American, African, Jewish, European and Asian heritage carry specific haplogroups and match people who have similar roots.

You may want to read this short article, 4 Kinds of DNA for Genetic Genealogy to understand the difference between Y, mitochondrial and autosomal DNA, what testing can tell you, and how they can help your genetic genealogy.

At a very basic level:

  • Y DNA testing tests the direct paternal (typically surname) line only, for males only. The Y chromosome is only passed from fathers to sons, so it is not divided nor mixed with the mother’s DNA. Females don’t have a Y chromosome, which is why they can’t test.
  • Mitochondrial DNA testing tests the direct matrilineal line only, for everyone, males and females both. The mitochondria is passed from mothers to all of her children, but is only passed on by females. It is not mixed with the father’s DNA, so it is not divided during the inheritance process.
  • Autosomal DNA testing tests all of your DNA, providing cousin matches and ethnicity estimates – but does not provide you with specifics about any individual line. You inherit half the autosomal DNA of each of your parents, so ancestral DNA diminishes by half in each generation. Autosomal testing is a great overview of all of your DNA lineages, but can’t tell you where any particular line comes from.

Testing the appropriate descendants of each ancestor allows us to build a DNA pedigree chart in order to determine the proven, specific heritage and origins of each individual line.

Here’s what my DNA Pedigree Chart looks like through my 8 great-grandparents where I’ve successfully obtained the Y and mitochondrial DNA of their descendants. Y and mitochondrial DNA, of course, has special properties and reaches back hundreds and thousands of years in time, because the Y and mitochondrial DNA is not diluted by the DNA of the other parent during inheritance.

I’ve converted the relationships in my pedigree chart above to an Ancestor Pedigree Chart, below, because we will be working with each individual and adding lines for other family members as we determine who we can test. You can click to enlarge the image.

In the Ancestor Pedigree Chart, shown above, there are 16 different people who all carry mitochondrial DNA, representing 8 different mitochondrial lines. Mitochondrial contributors, all women, shown in pink both carry and contribute mitochondrial DNA. Mothers contribute their mitochondrial DNA to the males, shown by pink hearts, but the men don’t pass it on. The daughters pass their mitochondrial DNA to all of their children.

There are 8 people, shown in blue, who carry and contribute Y DNA, representing 4 different Y lines.

Each mitochondrial and Y line of DNA has a story to tell that can’t be told any other way. Autosomal DNA does not provide specific information about the genesis or ethnicity of any particular line, but Y and mtDNA does. If you want to know specifically where, what part of the world, or what clan that particular ancestor descended from, Y and mitochondrial DNA may tell you.

The question becomes, who can be tested that is living today to obtain that specific information about each particular ancestor.

Of course, the answer of who to test to find the ancestral Y and mitochondrial DNA varies depending on the gender of the person, and where they are located in your tree.

If the person in the tree is no longer living, the answer about who to test may hinge on their siblings, and the descendants of their siblings or maybe cousins. Or perhaps you’ll need to go back up the tree a generation or two to find appropriately descended relatives to test.

For each of the individuals in this tree, I’m going to answer the question of whom to test to obtain their Y and mitochondrial DNA – and how to find a suitable candidate. Talking them into testing, however, is all up to you:)

If you haven’t tested your Y or mitochondrial DNA, and you want to, you can order those tests at Family Tree DNA.  I suggest a minimum of 37 markers for Y DNA. You can always upgrade later to 67 or 111 markers.  Regardless of your testing level, you’ll receive haplogroup estimates, matches and other information.  For mitochondrial DNA, order the full sequence test so you’ll receive your full haplogroup designation. Several Y and mitochondrial haplogroups originated in Asia, with some lines settling in Europe, some in Asia and some in the Americas – so you need as much information as you can extract from your DNA.

Please join me for the “Concepts – Who To Test?” Series – coming soon to a this blog, so stay tuned!!!

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

800 Articles Strong

800-strong

Today is something of a red-letter day. This is the 801st article published on this blog.

This blog, DNA-Explained, was christened on July 11, 2012 and will soon be 5 years old, as hard as that is to believe. In some ways, it feels like this blog has been around “forever” and in other ways, it feels like it’s very new, because there is always some interesting topic to write about.

Truthfully, I can’t believe I’ve written 800 articles. No wonder some of the letters are worn off of my keyboard. And it’s my second keyboard!

My original goal was one article per week, which would have been about 235 articles by now. I wasn’t sure I could accomplish that. It’s amazing what inspiration can do! I love genetic genealogy every bit as much today as I did then, if not more. What an incredibly exciting time to be alive with an unbelievable opportunity to participate in an unfolding field with new discoveries being made on an almost daily basis.

I had been considering a DNA blog when Spencer Wells, then Scientist in Residence at the National Geographic Society, suggested that I SHOULD author a blog. That encouragement was all it took to motivate me. Thanks so much Spencer for that final nudge!!!

spencer and me

Just 12 days after DNA-Explained’s launch, the Genographic 2.0 product was introduced and I was privileged to participate in that announcement.

I started writing articles in self-defense, truthfully, because I was receiving the same questions over and over again. I figured if I could write the answer once, I could then just point the next person with that same question to an answer that included graphics and illustrations and was a much better answer than I could provide in an e-mail.

Plus, repetitively recreating the same answer was a time-waster – and blogging to share publicly with the goal of helping lots of people learn seemed the perfect solution.

I had no idea, and I mean none, that DNA testing in the direct to consumer marketplace would explode like it has. I’m glad I started writing when I did, because there are ever-more people asking questions. That’s a good thing, because it means people are testing and learning what messages their DNA has for them.

Our DNA is the most personal record of our ancestors that we’ll ever have – and today more and more tools exist to interpret what those ancestors are telling us. We are still panning for gold on the frontier of science although we know infinitely more than we did a decade or 5 years ago, and we know less than we will 5 or 10 years from now. We are still learning every single day. That’s what makes this field so exciting, and infinitely personal.

Here’s part of what I said in my introductory article:

Genetic genealogy is a world full of promise, but it changes rapidly and can be confusing. People need to understand how to use the numerous tools available to us to unravel our ancestral history.

People also love to share stories. We become inspired by the successes of others, and ideas are often forthcoming that we would not have otherwise thought of.

In light of that, I’ve tried to include a wide variety of articles at every level so that there is something for everyone. I hope I’ve managed to make genetics interesting and shared some of my enthusiasm with you over the years.

In Celebration

To celebrate this 800 article-versary, I’m going to share a few things.

  • Article organization and how to find what you want
  • The 10 most popular articles of those 800
  • Two things people can do to help themselves
  • Articles I wish people would read
  • Questions asked most frequently

Then, I’m going to ask you what you’d like for me to write about in the future.

Articles Organization aka How To Find What You Want

Blogs allow you to group articles by both categories and tags, two ways of organizing your articles so that people and search engines can find them.

Each article is identified by categories. You can click on any of the categories, below, to see which articles fall into that category. These are also some of the keywords for the blog search feature.

I’ve also grouped articles by tags as shown on the sidebar of the blog. The larger text indicates tags with more articles.

800-tags

You can click on any of those as well (on the actual blog page) to view all the articles that fall into that tag group.

For example, one of my 52 Ancestor Stories would be tagged with “52 Weeks of Ancestors” but if it discussed Y DNA, that would be one of the categories selected.

At the end of every blog article, you can see the category or categories the article is posted under, tags and other pertinent information about that article.

800-end-of-article

The Top 10 Articles

  1. Proving Native American Ancestry Using DNA
  2. 4 Kinds of DNA for Genetic Genealogy
  3. Ethnicity Results – True or Not?
  4. Mythbusting – Women, Fathers and DNA
  5. Genealogy and Ethnicity DNA Testing – 3 Legitimate Companies
  6. How Much Indian Do I Have in Me???
  7. What is a Haplogroup?
  8. Thick Hair, Small Boobs, Shovel Shaped Teeth and More
  9. Ethnicity Testing and Results
  10. 23andMe, Ancestry and Selling Your DNA Information

The Two Things People Can Do To Help Themselves

  1. Search first.

Before asking a question, I wish people would try searching my blog for the answer. Using the search box in the upper right hand corner, the blog is fully key word searchable.

800-search

Furthermore, even if you can’t figure out the right key word to search, you can also find articles on my blog by searching for phrases using google.

2. Upload GEDCOM files.

Your DNA testing is only as good as the comparisons you can make, and the ancestors and ancestral links you can find. Please, please, PLEASE upload GEDCOM files to Family Tree DNA and GedMatch. If you don’t have a tree, you can create one at Family Tree DNA. Link your tree to your DNA results on Ancestry and share your results. 23andMe has no tree ability at this time.

The Articles I Wish People Would Read

In addition to some of the articles already listed in the top 10, I wish people would read:

Questions Asked Most Frequently

  • Questions relating to Native American heritage and testing.
  • Questions relating to ethnicity, especially when the results are unexpected or don’t seem to align with what is known or family oral history.
  • Overwhelmed newbies who receive results and don’t have any idea how to interpret what they’ve received, which is why I created the Help page.

The Future – What Articles Would You Like to See?

It’s your turn.

What topics would you like to see me cover in upcoming articles? Is there something in particular that you find confusing, or enticing, or exciting?

I’m not promising that I’ll write about every topic, and some may be combined, but articles are often prompted by questions and suggestions from readers.

And speaking of readers…

Thank You

A very big thank you to all of my subscribers and followers for making DNA-Explained so popular and such a success. You folks are amazing, infinitely giving and helpful. We really are a community!

thank-you

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

 

Native American and First Nations DNA Testing – Buyer Beware

Native DNA in Feathers

This week, a woman in North Carolina revealed that she descends from the extinct Beothuk tribe in Canada as a result of a DNA test from a Canadian DNA testing company. This has caused quite an uproar, in both genetic genealogy and Native American research communities, and has been resoundingly discredited by geneticists.

People’s motivation for wanting to know if they have Native heritage generally falls into the following categories:

  • Curiosity and a desire to confirm a family story
  • Desire to recover lost heritage
  • Desire to identify or join a tribe
  • Desire to obtain services provided to eligible tribal members, such as educational benefits
  • Desire to obtain benefits provided to eligible tribal members, such as a share of casino profits

Questions about DNA testing to reveal Native ancestry are the most common questions I receive and my Native DNA articles are the most visited on my website and blog.

Legitimate DNA Tests for Native Heritage

There are completely legitimate tests for Native ancestry, including the Y DNA and mitochondrial DNA tests for direct paternal (blue box genealogy line, below) and direct matrilineal lines (red circle genealogy line, below). Both Y and mitochondrial DNA have scientifically identified and confirmed haplogroups found only in Native Americans, as discussed in this article. Both Y and mitochondrial DNA at appropriate testing levels can identify a Native ancestor back in time thousands of years.

Y and mito

However, if the Native ancestor does not descend from the direct paternal or direct matrilineal lines, the only DNA test left is an autosomal test which tests all of your ancestral lines, but which can only reliably identify ancestral heritage for the past 5 or 6 generations in any of those lines due to recombination of DNA with the other parent in each generation. Autosomal tests provide you with percentage estimates of your ethnicity although they can vary widely between companies for various reasons. All three of these tests are available from Family Tree DNA as part of their normal product offering.

If you’d like to see an example of genealogy research combined with all three types of DNA testing for a Native Sioux man, please read about John Iron Moccasin.

Less Than Ethical DNA Tests for Native Heritage

Because of the desire within the consuming public to know more about their Native heritage, several specialty testing services have emerged to offer “Native American” tests. Recently, one, Accu-Metrics out of Canada has been highly criticized in the media for informing a woman that she was related to or descended from the extinct Beothuk tribe based on a match to a partial, damaged, mitochondrial sample from skeletal remains, now in housed in Scotland.

When you look at some of these sites, they spend a lot of time convincing you about the qualifications of the lab they use, but the real problem is not with the laboratory, but their interpretation of what those results mean to their clients – e.g. Beothuk.

Those of us who focus on Native American ancestry know unequivocally that “matching” someone with Native ancestry does NOT equate to being from that same tribe. In fact, we have people in the American Indian Project and various Native haplogroup projects who match each other with either Native Y or mitochondrial results who are tribally enrolled or descended from tribes from very different parts of the Americas, as far distant as Canada and South America.

Based on this 2007 paper, A Preliminary Analysis of the DNA and Diet of the Extinct Beothuk: A Systematic Approach to Ancient Human DNA, describing the analysis of the Beothuk remains, it appears that only the HVR1 region of the Beothuk skeletal remains were able to be partially sequenced. An HVR1 level only match between two people could be from thousands to tens of thousands of years ago.

According to Dr. Doron Behar’s paper, A ‘‘Copernican’’ Reassessment of the Human Mitochondrial DNA Tree from its Root, dating haplogroup formation, haplogroup C was formed about 24,000 years ago, give or take 5,000 years in either direction, and haplogroup X was formed about 32,000 years ago, give or take 12,000 years in either direction. There are individuals living in Europe and Asia, as well as the Americans who fall into various subgroups of haplogroup C and X, which are impossible to differentiate without testing beyond the HVR1 region. A match at the HVR1 level which only indicates C or X, without subgroups, could be from a very ancient common ancestor, back in Asia and does not necessarily indicate Native American heritage without additional testing. What this means is that someone whose ancestors have never lived outside of China, for example, would at the basic haplogroup level, C, match to the Beothuk remains because they shared a common ancestor 24,000 years ago.

Furthermore, many people are tribally enrolled whose mitochondrial or Y DNA would not be historically Native, because their tribal membership is not based on that ancestral line. Therefore, tribal membership alone is not predictive of a Native American Y or mitochondrial haplogroup. Matching someone who is tribally enrolled does not mean that your DNA is from that tribe, because their DNA from that line may not be historically Native either.

Tribes historically adopted non-Native people into the tribe, so finding a non-Native, meaning a European or African haplogroup in a tribal member is not unusual, even if the tribal member’s enrollment is based on that particular genealogical line. European or African DNA does not delegitimize their Native heritage or status, but finding a European or African haplogroup in a tribal member also does NOT mean that those haplogroups were historically Native, meaning pre-Columbian contact.

Worse yet, one company is taking this scenario a step further and is informing their clients that carry non-Native haplogroups that they have Native heritage because a group of their clients who “self-identified” as “Native,” meaning they believe their ancestor is Native, carry that haplogroup. The American myth of the “Indian Princess” is legendary and seldom do those stories pan out as accurate with DNA testing and traditional genealogical research. Basing one client’s identification as Native on another client’s family myth without corroboration is a mind-boggling stretch of logic. Most consumers who receive these reports never go any further, because they have achieved what they sought; “confirmation” of their Native heritage through DNA.

A match, even in the best of circumstances where the match does fall into the proven Native haplogroups does not automatically equal to tribal affiliation, and any company who suggests or says it does is substantially misleading their customers.

From the Accu-Metric site, the company that identified the woman as Beothuk:

Native American linkage is based on a sample comparison to a proven member of the group, which identifies specific tribal linkage.

New for 2016: We can also determine if you belong to the 56 Native tribes from Mexico.

The DNA results can be used in enrollment, disenrollment, claiming social benefits, or simply for a peace of mind. We understand the impact that this testing service has on the First Nation and Native American community and we try to use our expertise for the community’s overall interests.

From Dr. Steven Carr, a geneticist at Memorial University in St. John’s (Canada) who has studied the Beothuk:

We do not have enough of a database to identify somebody as being Beothuk, so if somebody is told [that] by a company, I think we call that being lied to.

I would certainly agree with Dr. Carr’s statement.

According to the 2007 Beothuk paper, the Beothuk mitochondrial DNA fell into two of the 5 typical haplogroups for Native American mitochondrial DNA, C and X. However, only portions or subgroups of those 5 haplogroups are Native, and all Native people fall someplace in those 5 haplogroup subgroups, as documented here.

The Beothuk remains would match, at the basic haplogroup level, every other Native person in haplogroup C or X across all of North and South America. In fact, the Beothuk remains match every other person world-wide at the basic haplogroup level that fall into haplogroups C or X.  It would take testing of the Beothuk remains at the full sequence level, which was not possible due to degradation of the remains, to be more specific.  So telling a woman that she matches the Beothuk was irresponsible at best, because those Beothuk remains match every other person in haplogroup C or X, Native or not.  Certainly, a DNA testing company knows this.

Accu-Metrics isn’t the only company stretching or twisting the truth for their own benefit, exploiting their clients. Dr Jennifer Raff, a geneticist who studies Native American DNA, discusses debunking what she terms pseudogenetics, when genetic information is twisted or otherwise misused to delude the unsuspecting. You can view her video here. About minute 48 or 49, she references another unethical company in the Native American DNA testing space.

Unfortunately, unethical companies are trying to exploit and take advantage of the Native people, of our ancestors, and ultimate of us, the consumers in our quest to find those ancestors.

Reputable DNA Testing

If you want to test for your Native heritage, be sure you understand what various tests can and cannot legitimately tell you, which tests are right for you based on your gender and known genealogy, and stay with a reputable testing company. I recommend Family Tree DNA for several reasons.

  • Family Tree DNA is the founding company in genetic genealogy
  • They have been in business since 1999
  • They are reputable
  • They are the only company to offer all three types of DNA tests
  • They offer matching between their clients whose DNA matches each other, giving you the opportunity to work together to identify your common link
  • They sponsor various free projects for customers to join to collaborate with other researchers with common interests

When evaluating tests from any other companies, if it sounds too good to be true, and no other company can seem to provide that same level of specificity, it probably is too good to be true. No company can identify your tribe through DNA testing. Don’t be a victim.

These three articles explain about DNA testing, and specifically Native DNA testing, and what can and cannot be accomplished.

For other articles about Native American DNA testing, this blog is fully key-word searchable by utilizing the search box in the upper right hand corner.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

Concepts – Segment Size, Legitimate and False Matches

Matchmaker, matchmaker, make me a match!

One of the questions I often receive about autosomal DNA is, “What, EXACTLY, is a match?”  The answer at first glance seems evident, meaning when you and someone else are shown on each other’s match lists, but it really isn’t that simple.

What I’d like to discuss today is what actually constitutes a match – and the difference between legitimate or real matches and false matches, also called false positives.

Let’s look at a few definitions before we go any further.

Definitions

  • A Match – when you and another person are found on each other’s match lists at a testing vendor. You may match that person on one or more segments of DNA.
  • Matching Segment – when a particular segment of DNA on a particular chromosome matches to another person. You may have multiple segment matches with someone, if they are closely related, or only one segment match if they are more distantly related.
  • False Match – also known as a false positive match. This occurs when you match someone that is not identical by descent (IBD), but identical by chance (IBC), meaning that your DNA and theirs just happened to match, as a happenstance function of your mother and father’s DNA aligning in such a way that you match the other person, but neither your mother or father match that person on that segment.
  • Legitimate Match – meaning a match that is a result of the DNA that you inherited from one of your parents. This is the opposite of a false positive match.  Legitimate matches are identical by descent (IBD.)  Some IBD matches are considered to be identical by population, (IBP) because they are a result of a particular DNA segment being present in a significant portion of a given population from which you and your match both descend. Ideally, legitimate matches are not IBP and are instead indicative of a more recent genealogical ancestor that can (potentially) be identified.

You can read about Identical by Descent and Identical by Chance here.

  • Endogamy – an occurrence in which people intermarry repeatedly with others in a closed community, effectively passing the same DNA around and around in descendants without introducing different/new DNA from non-related individuals. People from endogamous communities, such as Jewish and Amish groups, will share more DNA and more small segments of DNA than people who are not from endogamous communities.  Fully endogamous individuals have about three times as many autosomal matches as non-endogamous individuals.
  • False Negative Match – a situation where someone doesn’t match that should. False negatives are very difficult to discern.  We most often see them when a match is hovering at a match threshold and by lowing the threshold slightly, the match is then exposed.  False negative segments can sometimes be detected when comparing DNA of close relatives and can be caused by read errors that break a segment in two, resulting in two segments that are too small to be reported individually as a match.  False negatives can also be caused by population phasing which strips out segments that are deemed to be “too matchy” by Ancestry’s Timber algorithm.
  • Parental or Family Phasing – utilizing the DNA of your parents or other close family members to determine which side of the family a match derives from. Actual phasing means to determine which parts of your DNA come from which parent by comparing your DNA to at least one, if not both parents.  The results of phasing are that we can identify matches to family groups such as the Phased Family Finder results at Family Tree DNA that designate matches as maternal or paternal based on phased results for you and family members, up to third cousins.
  • Population Based Phasing – In another context, phasing can refer to academic phasing where some DNA that is population based is removed from an individual’s results before matching to others. Ancestry does this with their Timber program, effectively segmenting results and sometimes removing valid IBD segments.  This is not the type of phasing that we will be referring to in this article and parental/family phasing should not be confused with population/academic phasing.

IBD and IBC Match Examples

It’s important to understand the definitions of Identical by Descent and Identical by Chance.

I’ve created some easy examples.

Let’s say that a match is defined as any 10 DNA locations in a row that match.  To keep this comparison simple, I’m only showing 10 locations.

In the examples below, you are the first person, on the left, and your DNA strands are showing.  You have a pink strand that you inherited from Mom and a blue strand inherited from Dad.  Mom’s 10 locations are all filled with A and Dad’s locations are all filled with T.  Unfortunately, Mother Nature doesn’t keep your Mom’s and Dad’s strands on one side or the other, so their DNA is mixed together in you.  In other words, you can’t tell which parts of your DNA are whose.  However, for our example, we’re keeping them separate because it’s easier to understand that way.

Legitimate Match – Identical by Descent from Mother

matches-ibd-mom

In the example above, Person B, your match, has all As.  They will match you and your mother, both, meaning the match between you and person B is identical by descent.  This means you match them because you inherited the matching DNA from your mother. The matching DNA is bordered in black.

Legitimate Match – Identical by Descent from Father

In this second example, Person C has all T’s and matches both you and your Dad, meaning the match is identical by descent from your father’s side.

matches-ibd-dad

You can clearly see that you can have two different people match you on the same exact segment location, but not match each other.  Person B and Person C both match you on the same location, but they very clearly do not match each other because Person B carries your mother’s DNA and Person C carries your father’s DNA.  These three people (you, Person B and Person C) do NOT triangulate, because B and C do not match each other.  The article, “Concepts – Match Groups and Triangulation” provides more details on triangulation.

Triangulation is how we prove that individuals descend from a common ancestor.

If Person B and Person C both descended from your mother’s side and matched you, then they would both carry all As in those locations, and they would match you, your mother and each other.  In this case, they would triangulate with you and your mother.

False Positive or Identical by Chance Match

This third example shows that Person D does technically match you, because they have all As and Ts, but they match you by zigzagging back and forth between your Mom’s and Dad’s DNA strands.  Of course, there is no way for you to know this without matching Person D against both of your parents to see if they match either parent.  If your match does not match either parent, the match is a false positive, meaning it is not a legitimate match.  The match is identical by chance (IBC.)

matches-ibc

One clue as to whether a match is IBC or IBD, even without your parents, is whether the person matches you and other close relatives on this same segment.  If not, then the match may be IBC. If the match also matches close relatives on this segment, then the match is very likely IBD.  Of course, the segment size matters too, which we’ll discuss momentarily.

If a person triangulates with 2 or more relatives who descend from the same ancestor, then the match is identical by descent, and not identical by chance.

False Negative Match

This last example shows a false negative.  The DNA of Person E had a read error at location 5, meaning that there are not 10 locations in a row that match.  This causes you and Person E to NOT be shown as a match, creating a false negative situation, because you actually do match if Person E hadn’t had the read error.

matches-false-negative

Of course, false negatives are by definition very hard to identify, because you can’t see them.

Comparisons to Your Parents

Legitimate matches will phase to your parents – meaning that you will match Person B on the same amount of a specific segment, or a smaller portion of that segment, as one of your parents.

False matches mean that you match the person, but neither of your parents matches that person, meaning that the segment in question is identical by chance, not by descent.

Comparing your matches to both of your parents is the easiest litmus paper test of whether your matches are legitimate or not.  Of course, the caveat is that you must have both of your parents available to fully phase your results.

Many of us don’t have both parents available to test, so let’s take a look at how often false positive matches really do occur.

False Positive Matches

How often do false matches really happen?

The answer to that question depends on the size of the segments you are comparing.

Very small segments, say at 1cM, are very likely to match randomly, because they are so small.  You can read more about SNPs and centiMorgans (cM) here.

As a rule of thumb, the larger the matching segment as measured in cM, with more SNPs in that segment:

  • The stronger the match is considered to be
  • The more likely the match is to be IBD and not IBC
  • The closer in time the common ancestor, facilitating the identification of said ancestor

Just in case we forget sometimes, identifying ancestors IS the purpose of genetic genealogy, although it seems like we sometimes get all geeked out by the science itself and process of matching!  (I can hear you thinking, “speak for yourself, Roberta.”)

It’s Just a Phase!!!

Let’s look at an example of phasing a child’s matches against those of their parents.

In our example, we have a non-endogamous female child (so they inherit an X chromosome from both parents) whose matches are being compared to her parents.

I’m utilizing files from Family Tree DNA. Ancestry does not provide segment data, so Ancestry files can’t be used.  At 23andMe, coordinating the security surrounding 3 individuals results and trying to make sure that the child and both parents all have access to the same individuals through sharing would be a nightmare, so the only vendor’s results you can reasonably utilize for phasing is Family Tree DNA.

You can download the matches for each person by chromosome segment by selecting the chromosome browser and the “Download All Matches to Excel (CSV Format)” at the top right above chromosome 1.

matches-chromosomr-browser

All segment matches 1cM and above will be downloaded into a CSV file, which I then save as an Excel spreadsheet.

I downloaded the files for both parents and the child. I deleted segments below 3cM.

About 75% of the rows in the files were segments below 3cM. In part, I deleted these segments due to the sheer size and the fact that the segment matching was a manual process.  In part, I did this because I already knew that segments below 3 cM weren’t terribly useful.

Rows Father Mother Child
Total 26,887 20,395 23,681
< 3 cM removed 20,461 15,025 17,784
Total Processed 6,426 5,370 5,897

Because I have the ability to phase these matches against both parents, I wanted to see how many of the matches in each category were indeed legitimate matches and how many were false positives, meaning identical by chance.

How does one go about doing that, exactly?

Downloading the Files

Let’s talk about how to make this process easy, at least as easy as possible.

Step one is downloading the chromosome browser matches for all 3 individuals, the child and both parents.

First, I downloaded the child’s chromosome browser match file and opened the spreadsheet.

Second, I downloaded the mother’s file, colored all of her rows pink, then appended the mother’s rows into the child’s spreadsheet.

Third, I did the same with the father’s file, coloring his rows blue.

After I had all three files in one spreadsheet, I sorted the columns by segment size and removed the segments below 3cM.

Next, I sorted the remaining items on the spreadsheet, in order, by column, as follows:

  • End
  • Start
  • Chromosome
  • Matchname

matches-both-parents

My resulting spreadsheet looked like this.  Sorting in the order prescribed provides you with the matches to each person in chromosome and segment order, facilitating easy (OK, relatively easy) visual comparison for matching segments.

I then colored all of the child’s NON-matching segments green so that I could see (and eventually filter the matchname column by) the green color indicating that they were NOT matches.  Do this only for the child, or the white (non-colored) rows.  The child’s matchname only gets colored green if there is no corresponding match to a parent for that same person on that same chromosome segment.

matches-child-some-parents

All of the child’s matches that DON’T have a corresponding parent match in pink or blue for that same person on that same segment will be colored green.  I’ve boxed the matches so you can see that they do match, and that they aren’t colored green.

In the above example, Donald and Gaff don’t match either parent, so they are all green.  Mess does match the father on some segments, so those segments are boxed, but the rest of Mess doesn’t match a parent, so is colored green.  Sarah doesn’t match any parent, so she is entirely green.

Yes, you do manually have to go through every row on this combined spreadsheet.

If you’re going to phase your matches against your parent or parents, you’ll want to know what to expect.  Just because you’ve seen one match does not mean you’ve seen them all.

What is a Match?

So, finally, the answer to the original question, “What is a Match?”  Yes, I know this was the long way around the block.

In the exercise above, we weren’t evaluating matches, we were just determining whether or not the child’s match also matched the parent on the same segment, but sometimes it’s not clear whether they do or do not match.

matches-child-mess

In the case of the second match with Mess on chromosome 11, above, the starting and ending locations, and the number of cM and segments are exactly the same, so it’s easy to determine that Mess matches both the child and the father on chromosome 11. All matches aren’t so straightforward.

Typical Match

matches-typical

This looks like your typical match for one person, in this case, Cecelia.  The child (white rows) matches Cecelia on three segments that don’t also match the child’s mother (pink rows.)  Those non-matching child’s rows are colored green in the match column.  The child matches Cecelia on two segments that also match the mother, on chromosome 20 and the X chromosome.  Those matching segments are boxed in black.

The segments in both of these matches have exact overlaps, meaning they start and end in exactly the same location, but that’s not always the case.

And for the record, matches that begin and/or end in the same location are NOT more likely to be legitimate matches than those that start and end in different locations.  Vendors use small buckets for matching, and if you fall into any part of the bucket, even if your match doesn’t entirely fill the bucket, the bucket is considered occupied.  So what you’re seeing are the “fuzzy” bucket boundaries.

(Over)Hanging Chad

matches-overhanging

In this case, Chad’s match overhangs on each end.  You can see that Chad’s match to the child begins at 52,722,923 before the mother’s match at 53,176,407.

At the end location, the child’s matching segment also extends beyond the mother’s, meaning the child matches Chad on a longer segment than the mother.  This means that the segment sections before 53,176,407 and after 61,495,890 are false negative matches, because Chad does not also match the child’s mother of these portions of the segment.

This segment still counts as a match though, because on the majority of the segment, Chad does match both the child and the mother.

Nested Match

matches-nested

This example shows a nested match, where the parent’s match to Randy begins before the child’s and ends after the child’s, meaning that the child’s matching DNA segment to Randy is entirely nested within the mother’s.  In other words, pieces got shaved off of both ends of this segment when the child was inheriting from her mother.

No Common Matches

matches-no-common

Sometimes, the child and the parent will both match the same person, but there are no common segments.  Don’t read more into this than what it is.  The child’s matches to Mary are false matches.  We have no way to judge the mother’s matches, except for segment size probability, which we’ll discuss shortly.

Look Ma, No Parents

matches-no-parents

In this case, the child matches Don on 5 segments, including a reasonably large segment on chromosome 9, but there are no matches between Don and either parent.  I went back and looked at this to be sure I hadn’t missed something.

This could, possibly, be an instance of an unseen a false negative, meaning perhaps there is a read issue in the parent’s file on chromosome 9, precluding a match.  However, in this case, since Family Tree DNA does report matches down to 1cM, it would have to be an awfully large read error for that to occur.  Family Tree DNA does have quality control standards in place and each file must pass the quality threshold to be put into the matching data base.  So, in this case, I doubt that the problem is a false negative.

Just because there are multiple IBC matches to Don doesn’t mean any of those are incorrect.  It’s just the way that the DNA is inherited and it’s why this type of a match is called identical by chance – the key word being chance.

Split Match

matches-split

This split match is very interesting.  If you look closely, you’ll notice that Diane matches Mom on the entire segment on chromosome 12, but the child’s match is broken into two.  However, the number of SNPs adds up to the same, and the number of cM is close.  This suggests that there is a read error in the child’s file forcing the child’s match to Diane into two pieces.

If the segments broken apart were smaller, under the match threshold, and there were no other higher matches on other segments, this match would not be shown and would fall into the False Negative category.  However, since that’s not the case, it’s a legitimate match and just falls into the “interesting” category.

The Deceptive Match

matches-surname

Don’t be fooled by seeing a family name in the match column and deciding it’s a legitimate match.  Harrold is a family surname and Mr. Harrold does not match either of the child’s parents, on any segment.  So not a legitimate match, no matter how much you want it to be!

Suspicious Match – Probably not Real

matches-suspicious

This technically is a match, because part of the DNA that Daryl matches between Mom and the child does overlap, from 111,236,840 to 113,275,838.  However, if you look at the entire match, you’ll notice that not a lot of that segment overlaps, and the number of cMs is already low in the child’s match.  There is no way to calculate the number of cMs and SNPs in the overlapping part of the segment, but suffice it to say that it’s smaller, and probably substantially smaller, than the 3.32 total match for the child.

It’s up to you whether you actually count this as a match or not.  I just hope this isn’t one of those matches you REALLY need.  However, in this case, the Mom’s match at 15.46 cM is 99% likely to be a legitimate match, so you really don’t need the child’s match at all!!!

So, Judge Judy, What’s the Verdict?

How did our parental phasing turn out?  What did we learn?  How many segments matched both the child and a parent, and how many were false matches?

In each cM Size category below, I’ve included the total number of child’s match rows found in that category, the number of parent/child matches, the percent of parent/child matches, the number of matches to the child that did NOT match the parent, and the percent of non-matches. A non-match means a false match.

So, what the verdict?

matches-parent-child-phased-segment-match-chart

It’s interesting to note that we just approach the 50% mark for phased matches in the 7-7.99 cM bracket.

The bracket just beneath that, 6-6.99 shows only a 30% parent/child match rate, as does 5-5.99.  At 3 cM and 4 cM few matches phase to the parents, but some do, and could potentially be useful in groups of people descended from a known common ancestor and in conjunction with larger matches on other segments. Certainly segments at 3 cM and 4 cM alone aren’t very reliable or useful, but that doesn’t mean they couldn’t potentially be used in other contexts, nor are they always wrong. The smaller the segment, the less confidence we can have based on that segment alone, at least below 9-15cM.

Above the 50% match level, we quickly reach the 90th percentile in the 9-9.99 cM bracket, and above 10 cM, we’re virtually assured of a phased match, but not quite 100% of the time.

It isn’t until we reach the 16cM category that we actually reach the 100% bracket, and there is still an outlier found in the 18-18.99 cM group.

I went back and checked all of the 10 cM and over non-matches to verify that I had not made an error.  If I made errors, they were likely counting too many as NON-matches, and not the reverse, meaning I failed to visually identify matches.  However, with almost 6000 spreadsheet rows for the child, a few errors wouldn’t affect the totals significantly or even noticeably.

I hope that other people in non-endogamous populations will do the same type of double parent phasing and report on their results in the same type of format.  This experiment took about 2 days.

Furthermore, I would love to see this same type of experiment for endogamous families as well.

Summary

If you can phase your matches to either or both of your parents, absolutely, do.  This this exercise shows why, if you have only one parent to match against, you can’t just assume that anyone who doesn’t match you on your one parent’s side automatically matches you from the other parent. At least, not below about 15 cM.

Whether you can phase against your parent or not, this exercise should help you analyze your segment matches with an eye towards determining whether or not they are valid, and what different kinds of matches mean to your genealogy.

If nothing else, at least we can quantify the relatively likelihood, based on the size of the matching segment, in a non-endogamous population, a match would match a parent, if we had one to match against, meaning that they are a legitimate match.  Did you get all that?

In a nutshell, we can look at the Parent/Child Phased Match Chart produced by this exercise and say that our 8.5 cM match has about a 66% chance of being a legitimate match, and our 10.5 cM match has a 95% change of being a legitimate match.

You’re welcome.

Enjoy!!

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

John Iron Moccasin, The Story of a Sioux Man

Occasionally, the project administrators of the American Indian project are presented with a rare opportunity to test an individual who is either full-blooded Native or nearly so. Recently, a Native Sioux man, John Iron Moccasin, born Earl White Weasel, stepped forward.

In order to facilitate testing, project members and others contributed funds with the agreement that we could publish John’s results and story. Now that the original tests are complete and we are publishing his results, we would like to upgrade John’s Y markers to 111 (from 37) and add the Big Y test – so if you’re inclined to contribute to the American Indian Project for this advanced testing – you can do so by clicking here.

But first, perhaps you’d like to hear John’s story. The results of the research into John’s history, both genealogically and genetically are fascinating. I hope you’ll get a cup of coffee or tea and enjoy this journey. Come along – we’re going on an adventure to South Dakota and we’ll be visiting the Sioux people!

In the Beginning…

A few months ago, John Iron Moccasin was talking to his friend and told her that he would like to share not only his oral history, but his genealogy and genetic history, with his daughter. He didn’t know how to go about doing either, but that friend, Pam, did, and she turned to me.

John was born as Earl White Weasel on Eagle Butte Reservation in South Dakota. He then lived at Cherry Creek Reservation in South Dakota. After adoption, he relocated to Pine Ridge Reservation, Kyle Reservation and then Oglala Reservation.

Unlike many adoptees, John always knew the identity of his birth parents and has given permission to use both his birth and adopted surnames. He takes pride in both, as well as his heritage. However, since John’s genetic genealogy is connected only with his biological parents, that’s where this article will focus.

Both of John’s biological parents belonged to the Cheyenne Sioux tribe. His birth father was Timothy Urban White Weasel and his birth mother was Martha Hale.

John is tribally enrolled with the Cheyenne Sioux based on his birth parents. John’s card shows his “degree of blood” to be at least 15/16ths.

Let’s take a look at tracking both John’s maternal and paternal ancestry. Many people ask how to work with Native records, and this article will follow my step-by-journey with both John’s traditional genealogy as well as his genetic genealogy, tracking each line back in time. But first, let’s look at the history of the Sioux people.

The Sioux

The Sioux are groups of Native American tribes and First Nations peoples in North America. The term can refer to any ethnic group within the Great Sioux Nation or to any of the nation’s many language dialects. The Sioux comprise three major divisions based on language divisions: the Dakota, Lakota, and Nakota.

The Santee Dakota reside in the extreme east of the Dakotas, Minnesota and northern Iowa. The Yankton and Yanktonai Dakota reside in the Minnesota River area. They are considered to be the middle Sioux, and have in the past been erroneously classified as Nakota. The actual Nakota are the Assiniboine and Stoney of Western Canada and Montana. The Lakota, also called Teton are the westernmost Sioux, known for their hunting and warrior culture.

The Treaty of Fort Laramie in 1868 established the Great Sioux Reservation, shown below, much of which has been whittled away today.

Today, the Sioux maintain many separate tribal governments scattered across several reservations, communities, and reserves in North Dakota, South Dakota, Nebraska, Minnesota, and Montana in the United States; and Manitoba and southern Saskatchewan in Canada.

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By User:Nikater – Own work by Nikater, submitted to the public domain. Background map courtesy of Demis, http://www.demis.nl., Public Domain, https://commons.wikimedia.org/w/index.php?curid=2309029

The Dakota are first recorded to have resided at the source of the Mississippi River during the seventeenth century. The source of the Mississippi trickled out of Lake Itasca in present day South Clearwater, Minnesota. On the map below, you can see that location as well as Eagle Butte, to the west (larger white circle in South Dakota), some 300 or more miles as the crow flies, where John Iron Moccasin was born. The third location, Wilsall, Montana, on further west (red balloon), is where the remains of the 12,500 year old Anzick Child were found with Clovis tools.

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By 1700 some Sioux had migrated to present-day South Dakota. John’s Native ancestors were born in North Dakota, South Dakota, Montana, Nebraska and reportedly, Canada.

Late in the 17th century, the Dakota entered into an alliance with French merchants. The French were trying to gain advantage in the struggle for the North American fur trade against the English, who had recently established the Hudson’s Bay Company.

The first recorded encounter between the Sioux and the French occurred when Radisson and Groseilliers reached what is now Wisconsin during the winter of 1659-60. Later visiting French traders and missionaries included Claude-Jean Allouez, Daniel Greysolon Duluth, and Pierre-Charles Le Sueur who wintered with Dakota bands in early 1700. In 1736 a group of Sioux killed Jean Baptiste de La Vérendrye and twenty other men on an island in Lake of the Woods. However, trade with the French continued until after the French gave up North America in 1763.

For the most part, Sioux contact with Europeans was very limited until in the 1800s, and then, it turned deadly in a series of “wars” as the Sioux tried to protect their land and way of life. Europeans were equally as determined to eradicate the Indians, take their land and eliminate their way of life – and ultimately – they succeeded by containing the Sioux on reservations.

Records, other than oral history in the Sioux tongue, didn’t begin until Europeans began keeping them, so our earliest genealogical records of the Sioux only reach back into the 1800s. Thankfully, genetic records can reach back infinitely into time.

Let’s visit John Iron Moccasin’s ancestors, beginning with John’s paternal line.

The White Weasel Line

John’s father was Timothy Urban White Weasel, born August 1, 1939 to Oscar White Weasel and his wife, Esther (also called Estella) Ward. Timothy died March 28, 2004 in Eagle Butte, Dewey County, SD, the same location where he was born.

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John’s grandfather, Oscar White Weasel is listed as a farmer in the 1930 census in Ziebach County, South Dakota, in Township 8, district 59 as a full blood Sioux male with a note “74-5,” speaking Sioux, as is his wife, Esther, age 24. They have been married 5 years and have two children, Margie age 4 & 9/12 and Beatrice, age 2 & 5/12th. Oscar is a veteran.

Please note that you can click to enlarge any graphic.

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This means John’s grandfather was born about 1898 and his grandmother about 1906. It should be noted that many traditional Native people have only a general idea of when they were born.

The US Department of Veterans Affairs Death File shows that Oscar Weasel was born on Feb. 22, 1898 and died on February 12, 1979. His military service was from March 28, 1917 to May 12, 1919.

The 1940 census from the same location shows Oscar J. White Weasel, age 42, wife Esther M., age 38, both Indian, both born in South Dakota, both educated through 7th grade, with 5 children including baby Urban J. White Weasel, age 7/12th. They live in Cherry Creek in Ziebach County, SD in the same place they lived in 1935.

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The Rapid City, SD obituary index shows that two obituaries for Oscar exist.

Weasel, Oscar J. 80 12 Feb 1979 Fort Meade, SD BHN 14 Feb 1979 p.31

16 Feb 1979 p.5

BHN means that Oscar is buried in the Black Hills National Cemetery. Find-A-Grave shows that he is buried in Section C, site 455 and that he was a PFC in WWI.

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im-black-hillsThe Social Security Claims Index shows that Oscar’s wife was Esther Ward and their child that filed the claim is Beatrice Louise Janis.

The 1927 Indian Census of the Cheyenne River Sioux Agency provides a little more information.

Joseph, also known as Oscar White Weasel is listed as born in 1898 and with two numbers instead of an English name. 322986 and 328110. I suspect these are the governmental identification numbers assigned to his parents when they were paid from the settlement fund – although one of those numbers could he his. His wife is listed as born in 1903 and as Mrs. Joseph White Weasel, nee Esther Ward, and she has one number listed in place of English name, 359087. Their daughter Margie is listed as born in 1925 and has no number listed by her name. There are no additional White Weasel individuals listed.

The 1925 Indian Census (below) shows us that he is listed as Joseph with Oscar penciled in above the name, with the number 322986 beside his name – which is evidently his number.

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The numbers probably related to the numbers assigned to Indians on the Dawes Rolls resulting from the Dawes Act of 1887 which allotted tribal lands in severalty to individual tribal members in exchange for Native Americans becoming US citizens and giving up some forms of tribal self-government.

In the South Dakota 1925 census, Joseph White Weasel is listed as married in 1924 and as Catholic. The South Dakota Marriages lists them as having married on October 18, 1924 in Cherry Creek.

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Many of the Native people were “converted” to Catholicism by missionaries. The French were Catholic and the traders in this region and throughout the Great Lakes were French.

The 1900 federal census (below) lists Joseph White Weasel, born in 1898 as the son of Charley White Weasel born in April of 1866 in South Dakota. They are living on the Cheyenne River Indian Reservation, district 48 in Dewey, County, South Dakota. Joseph’s mother is “Follows” and she was born in July of 1869 in Montana, as were both children. They have been married 12 years, had 5 children, and 2 are living. Joseph’s older brother is Wakes (probably Makes) Believe his (probably he’s) Running. Charley is listed as “Indian Police” and Follows is listed as “Ration Indian.” They have not attended school, cannot read or write and do not speak English.

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The bottom of the census document includes an area called “special inquiries relating to Indians.”

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This entire family is listed as Sioux, with no white blood. The mother and father of both Charley and Follows are listed as Sioux as well. They are not polygamous and they lived in a fixed, as opposed to moveable, structure. In other words, a “house” of some sort, not a teepee.

Polygamy was considered a grave sin by most Christian religions, and clearly someone still practicing the Native ways, which includes both polygamy and living in teepees, was highly encouraged to abandon those practices.

Note in the Indian census as late as 1902, some households are still listed with wife 1 and wife 2. It’s impossible to tell which child was born to which wife.

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Also note that the Native name and English name may have nothing to do with each other. They are not always literal translations. Please also note that Follows Him, above, is not the same person as Follows.

Christianity, and specifically Catholicism, along with “civility,” meant taking English names and living in established locations in structures. These behaviors were strongly encouraged and then forced upon the Native people with the Indian Citizenship Act of 1924 when their children were sent to “boarding schools” to learn the white ways, renamed, and it became illegal to practice the Native ways, including spiritual practices, powwows and speaking their own language. These restrictions lasted until the Native American Languages Act of 1990 which once again allowed Native people to speak their own language and the 1978 American Indian Religious Freedom Act allowing Native people once again to hold events such as powwows and practice their own belief system.  Unfortunately, the half century plus between 1924 and 1978/1990 successfully eroded and destroyed much of the Native cultural heritage.

Follows continues to be listed in the Indian census documents. 1895 is shown below.

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The 1897 Indian census (below) shows Follows and White Weasel with Makes Believe he is Running and a new child, aged 2. This child is not yet named, which makes sense in the Indian culture because children are not named until they “earn” a name of some sort. In some tribes, names are changed as new names are earned.

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The family is also shown in the Indian Census of 1899 (below) where Joseph has been named, in 1900, in 1902 when Lucy has been born, in 1903, in 1904, in 1906 when Lucy is no longer with them, and in 1907.

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The June 30, 1909 Indian Census shows Follows, age 40, but White Weasel is gone and she is shown with both sons, below.

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The 1910 federal census shows a Louise Weasel on the Cheyenne River Indian Reservation, with sons Peter age 17 and Oscar, age 11. I don’t know if this is the same family with white names, or this is a different family. I suspect that Follows has been “renamed” Louise for the federal census document.

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The 1910 Indian census shows Follows with both boys again as well as in 1911, 1913, 1914, 1915, 1917.  In 1918, Follows is shown with only Joseph.

I cannot find either Follows or Joseph (Oscar) White Weasel in the 1920 census, although he was clearly living because he married in 1924. It’s unclear when Follows died.

The Ward Line

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John’s grandmother, Esther Ward is listed as Esther White Weasel born in 1904 on the 1945 South Dakota Census, with both of her parents born in South Dakota.

On the 1910 federal census, Esther Ward is 6 years old living with her father, Alfred Ward, age 32, married 13 years, and his wife Nellie age 28. They have another daughter, Mary, age 12 and (apparently) a son, Alec Chasing Hawk, age 2. Alec’s father is listed as having been born in Montana and mother South Dakota, white everyone else and their parents are listed as born in South Dakota – so Alec is a bit of an enigma. They also live with a man I would presume to be Alfred Ward’s’s father, although he could be Nellie’s father, as he is listed only as “father” but generally that is the relationship to the head of the household. Jerome Chasing Hawk, age 78, so born in about 1832, widowed, Sioux, a Ration Indian. However, we later discover that Alfred Ward’s father is Clarence “Roan Bear” Ward and his mother is Estella DuPris, so the identity of Jerome Chasing Hawk is quite a mystery.

Ration Indian means that they are receiving rations from the Bureau of Indian affairs, often in exchange for land traded by the tribe.

Alfred raises stock and both Alfred and Nellie can read and write, but Jerome cannot.

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In the special inquiries section, Alfred Ward is listed as ¾ Indian and ¼ white, married once, not living in polygamy, received an allotment in 1908 and is living on his own land.

Nellie is listed as full Indian, received an allotment in 1909 and has been married once.

Jerome Chasing Hawk is listed as full, married twice, not living in polygamy, and received an allotment in 1903. He is not living on his own land.

The 1900 federal census shows Chasing Hawk, a widower, as the father-in-law of Dirt Kettle, whose wife is Woman Eagle. Chasing Hawk is 68 and was born in May of 1832 in South Dakota. His father was born in an unknown location and his mother was born in North Dakota. He is a Ration Indian and does not read, write or speak English. In the special inquiries section, Chasing Hawk is noted with other name as “Cetan, unknown” and that he is full Native.

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I did not solve the mystery of Chasing Hawk’s relationship to this family.

If Alfred Ward is indeed ¼ white, then John Iron Moccasin is 1/32nd white, assuming all other ancestors were full Native.

The 1900 federal census shows Alfred Ward, age 22, with wife Pretty Voice, age 16 and daughter Irelia Ward, age 1.

Pretty Voice appears to be Nellie’s Native name.

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In the special inquiries section, Alfred is listed with both parents being Sioux, but listed as half white. Pretty Voice is listed as Sioux, all Indian with no white. He can speak English, she cannot. Alfred is shown in the photo below.

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On the 1925 Indian Census Roll, Alfred and Pretty Voice are both shown. He has number 246235 or 246285 next to his name and she has 248261 beside her name. They have 3 children.

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On the 1931 Indian Census Roll, Joseph White Weasel is listed with his wife, Esther, with their roll numbers and the identification numbers of their allotment, annuity and identification numbers.

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On the 1895 Indian census, Pretty Voice is listed as the child of Hump and White Calf is listed as Hump’s wife, although we will see in a minute why that may not mean that White Calf is Pretty Voice’s mother.

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This is a very interesting development, because Hump and White Calf are also in John Iron Moccasin’s mother’s line, as are Clarence Ward and Estella DuPris.

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The 1886 Indian Census shows Hump, age 45, with wife Beautiful Hail, age 26, and daughter Pretty Voice age 3 and Her Voice, age 2. This strongly suggests that Pretty Voice’s mother was Beautiful Hail and not White Calf.

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The 1891 Indian Census labeled “Sioux of different bands” shows Hump, age 43, his wife designated only as “Mrs.” age 21, With Pretty Voice, age 9, Sun age 6 and Hope or Hoop age 2.

The 1892 Indian Census shows that Hump, age 42, married to White Calf, with daughter Pretty Voice, age 11, Sun age 8 and Hope age 2. Her Voice is not with the family, so presumably has died.

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Pretty Voice is reported on another tree maintained by YanktonSiouxTribe, who indicates they are a professional genealogist, to be the daughter of Chief Hump, friend and mentor to Crazy Horse. YanktonSiouxTribe reports that Pretty Voice married Alfred Ward, son of Roan Bear also known as Clarence Ward and Estella Dupris, the daughter of Fred Dupris and Good Elk Woman whose photo is shown below.

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Good Elk Woman

In the 1895 Indian Census, Alfred Ward is shown living with his parents, Clarence Ward and Estelle Ward, ages 44 and 40, respectively. They would have been born in 1851 and 1855. Clarence and Estelle’s youngest son, Willie, is also John’s ancestor through his mother’s line, having married Hope (Dora) Hump.

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It’s interesting to note in reviewing the Indian census records that in the mid-1890s, many Native people did not have an English name. Some had both, but far less than half in this tribe. However, by the 1920 federal census, they all had white names.

The 1900 census shows us that Clarence Ward was born in July of 1850 in Nebraska and his parents were both born in South Dakota. He is listed as Missionary R and his wife is listed as a Ration Indian. The “R” is noted beside a number of occupations, so I would presume he is a missionary and the R may indicate “ration Indian” as well. They have been married 21 years and she has had 5 children, 4 of whom are living.

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In the special inquiries section, Clarence is listed as Sioux, as are his parents. Estella and her parents are also listed as Sioux, but she is listed as one half Native.

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In 1920, Clarence Ward was living, age 67, no occupation, wife Stella, age 64. Both were born in South Dakota and are living on the Cheyenne River Indian Reservation in SD.

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Clarence is reported to have died in 1933.

Stella, or Estella DuPris, was born in August 1854 to Frederick DuPris and Good Elk Woman and died on July 6, 1927. Stella married Clarence Ward (shown below), who was born in 1851 in Nebraska.

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In the 1886 Indian Census, Clarence is shown as 35, Estelle as 31 and Alfred as 9.

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The 1900 Federal census shows Clarence as a Missionary, Estelle as born in South Dakota, her father born in France and her mother born in South Dakota.

DuPris Line

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Stella’s father, Frederick Dupris, was born in 1813 in Quebec City, Quebec and died in 1898. He had 10 children with Good Elk Woman between 1845 and 1870. He died on June 16, 1898 in South Dakota. Good Elk Woman, also known as Mary Ann DuPris, died on February 13, 1900.

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Photo of Fred DuPris and his wife, Good Elk Woman and Son, Xavier Dupris, courtesy, South Dakota Historical Society.

In case there is any question about whether Fred DuPris was 100% white, the 1900 census lists his son, Fred Dupris as Sioux, father white, mother Sioux and he being one half Native. This, of course, indicates that Fred Sr. was all white.

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In the Indian Census of 1894, Good Elk Woman is listed as age 68 and is living with her daughter.

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Good Elk Woman was the daughter of One Iron Horn born about 1805 in South Dakota and Red Dressing born about 1810. Good Elk Woman was originally married to hereditary chief, Henry Makes Room and had a son, Henry Makes Room Junior.

The following information was provided by Calvin Dupree.

“The First Dupree Into South Dakota”

Frederick Dupuis came from Longueil, Quebec to Kaskaskia, Illinois and from there to the Cheyenne River area. One Dupuis brother, Pierre (known as Peter), went on up into Montana where he married an Assiniboin Sioux woman.

A French-Canadian, Fred Dupuis arrived at Fort Pierre in 1838 and was in employee of the American Fur Company under Pierre Choteau, Jr. Letters from the winter of 1861 were written to Charles Primeau from Fred Dupuis by M. C. Rousseau at the mouth of Cherry Creek. The letters were concerned with reports of the Indian bands and the number of buffalo robes Fred was sending in and a list of the materials he needed for trading and maintaining his small outpost at the mouth of Cherry Creek. The trader (Fred) was concerned that the buffalo were becoming scarce and that the Indians and their horses were “poor”.

By 1860, we must assume that Fred was married and busy with the affairs of a husband and father. He married a Minniconjou, Good Elk Woman, who became Mary Ann Dupuis. She had one son, Henry Makes Room, from a previous marriage who was adopted by Fred. Mary was the daughter of One Iron Horn and Red Dressing. Some elders in the family remember that Mary was from Cherry Creek. Mary and Fred had nine children. They were: Peter; Maggie (Fisherman); Esther (Ward); Edward; David Xavier; Alma (Blue Eyes); Fred, Jr.; Josephine (Vollin); Vetal; and Marcella (Carlin). “Not one of whom could speak English, with the exception of Edward, who was a student at Hampton, Va.”

After being an independent trader for some time (and probably as the buffalo dwindled and the Indians were put on reservations) Fred became a stock grower. He built the family home in a beautiful wooded flat on the north side of the Cheyenne River, thirty-five miles west of where it emptied into the Missouri. The patriarchal home was described as being 20 feet by 60 feet, and built of cottonwood logs. As each son or daughter married, a new small log house (called a tipi by the family) was built. These homes had dirt floor and gumbo roofs and were placed in a row near the main house. In addition there were usually a dozen tipis nearby, pitched by the full blood relatives of Mary Dupuis. The living arrangement was truly communal; the women had a large vegetable garden; the men worked the stock; all the cooking and eating was done in one cabin. One of the women baked all the bread, another cooked the meat and vegetables, and another made coffee and served the food. Three times a day 52 people ate together, along with any strangers or friends who might happen along.

The Dupuis home was known as a place for sharing good times and good food in the true Indian way. This was the era of government ration dispensing and all 52 of the family members collected their share which was hauled home in wagons from Fort Bennett, even though Old Fred was reputed to be wealthy with “several thousand head of cattle and 500 horses, a small herd of domesticated buffalo and a large amount of other property.”

The marriage of Marcella Dupuis, Old Fred’s youngest daughter, to Douglas F. Carlin, a non-Indian, of Pierre must have been a noteworthy event since newspapers from Deadwood and Pierre covered the event. Mr. Carlin was noted as the issue clerk at Cheyenne Agency. The ceremony was performed at the Dupuis home on the Cheyenne River with many important persons from the city, including the Pierre City Council, and unknown numbers of Sioux present. Forty fat steers were to be roasted. All the wedding gifts were put on exhibition after the supper, the most impressive being five hundred head of cattle and fifty ponies from Old Fred, father of the bride, and a decorated buffalo robe from sisters of the bride. The Sioux dancing continued for three days with the only interruption being a pause for more eating every three hours.

The Dupuis family’s contribution to saving the buffalo.

In 1883 (or possibly earlier) Old Fred and some of his sons and possibly Basil Clement (Claymore) went on a hunt for some buffalo calves in order to start a herd. By this time the great “surrounds” of the past were over and I can imagine that the desire to preserve at least a few of these animals, so necessary and so sacred to the Indian people, was strong. The group headed northwest from the Cheyenne River and was gone for many months and in Montana, or near Slim Buttes (reports differ), they located a small herd. They finally secured five calves (one report says nine), which were loaded into wagons brought along for that purpose. The calves were taken back to Cheyenne River.

By 1888 from this small start the Dupuis had nine pure-blood buffaloes. By the time of Old Fred’s death in 1898 the herd had grown considerably, and was purchased by James (Scotty) Philip of Fort Pierre. By 1918 (the herd) had increased to approximately 500 head. The State of South Dakota purchased 46 of these buffalo and transferred them to the State Game Park in Fall River County. Hearsay has it that Scotty Philip sold buffalo to other states and parks also, spreading the original Dupuis stock back into many areas where the buffalo once roamed free by the millions.

Old Fred died in 1898 at about age 80. Then, as now, a death was the occasion for sharing through a Give-Away of all the deceased’s belongings. From Aunt Molly Dupris Annis Rivers, Old Fred’s grand-daughter, I have heard the colorful story of how some of the Dupuis wealth was distributed. It is said that according to Lakota custom, any one who happened by was entitled to a gift and this even included a group of Crow Indians, traditional enemies of the Sioux since anyone can remember. The Crows were invited to join the other guests as they filed by a horse whose saddle bags had been filled with silver dollars. Each person took a silver dollar until they were gone; the next person in line was given the saddle, and the last person received the horse. And in this way, and probably by several other methods, Old Fred’s money and property were shared with the people. None of his oft mentioned wealth was inherited by any of his family.

Records indicate that Good Elk Woman, Mary Dupuis, died in 1900 at the home of her daughter, Mrs. Tom (Alma) Blue Eyes. One can only wonder about her life after Old Fred died, just as one wonders about her years of living, first as a child at Cherry Creek, then as a young wife of Makes Room and finally as Mary Ann Dupuis, mother of nine half French and half Lakota children. No stories about Mary have come down to me. Her life during the early time of tragedy and defeat for the Indian people cannot have been an easy one.

Old Fred and Mary, and many of their descendants, are buried in the Dupuis Cemetery on the hill above the river flat where their family home once was. Nearby is the old ”Buffalo Church”.

Old Fred and Mary may be gone, but South Dakota will not forget them. Dupree Creek runs into Rudy Creek and then into the Cheyenne River near the old home site, and the (town) of Dupree is located about 40 miles north of Cherry Creek where Old Fred carried on his fur trading. Just west of the Dupuis cemetery and the old church, in a draw filled with wild plums and chokecherries, the Dupree Spring (called the Circle P Spring, or Garrett Spring today) still furnishes clear, sweet water.

Imagine the hundreds of trips made to this spring, winter and summer, to haul water for the Dupuis family living down the hill by the river in the 1800’s.

The name, though changed from Dupuis to Dupris and in some cases to Dupree, has been carried all over South Dakota and to probably every state in the U.S. by their hundreds of descendants.

Calvin Dupree is the son of Adelia Fielder and Jonas E. Dupris; son of Sarah Red Horse and Frank Dupris; son of Harriet Cadotte and Xavier (David) Dupuis; son of Mary Ann Good Elk Woman and Frederick Dupuis. Calvin Dupree is presently a member of the faculty of Education at the University of Lethbridge in Alberta, Canada.

According to Suzanne DuPree, a descendant, Fred DuPris (in later generations spelled DePree), and Good Elk Woman are buried in the DuPris Memorial Cemetery on the hill above the river flat where their family one was once location, near the old “Buffalo Church.”

FindAGrave lists Fred DuPris’s birth date as September 5, 1819 and his death as July 16, 1898. His wife, Mary Ann, born as Good Elk Woman, is shown as being born in 1824 and passing over on February 13, 1900. The maps below are from FindAGrave.

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The Sioux Chief, Hump’s Line

John descends from Chief Hump twice, apparently through two different wives; Beautiful Hail and White Calf. John Iron Moccasin’s family information indicates that Hump had 4 wives: Good Voice/Good Woman, Brings Her, Stands As A Woman and Bessie/White Calf Woman. The census provides information about Beautiful Hail and White Calf, but we have no further information about Humps’s other two wives.

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Hump, also known as Thomas Hump, lived until December 11, 1908 where he died in Cherry Creek, SD.

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Photo courtesy of the South Dakota State Historical Society

Born in Montana, Hump became a leader of the Cherry Creek Band of Minneconjou Sioux. In 1876 he fought in the Battle of the Rosebud against Gen. Crook, shown below in the wood engraving below depicting the Sioux charging Colonel Royall’s attachment on June 17th.

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Hump also fought on Calhoun Hill in the Battle of the Little Big Horn with Crazy Horse, Gall and others against Custer and the 7th Calvary on June 25th where he received a bullet wound in his leg, according to the National Park Service.

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The Lakota Museum and Cultural Center tells us the following about Hump.

Etokeah, a Minniconjou Lakota war chief, was a great leader. He is especially known for his skills during the 19th Century Lakota-US Government battles. His exact birth date and facts of parentage were not recorded. However, he first came into public notice in 1866. Then, he led the charge against Captain William Fetterman’s soldiers outside Fort Phil Kearney in Wyoming.

Hump did not sign the Treaty of Fort Laramie in 1866. Because of his action, he was deemed a hostile or “non-treaty” chief by the US Government. He was a comrade-in-arms of Crazy Horse, Red Cloud and other great Sioux chiefs of the period. In 1876, he led his warriors into battle against Generals George Crook and George Custer.

After the defeat of the Sioux in the 1880s, he briefly lived in Canada. He eventually returned to the United States but remained hostile to the whites. In company with most of the Sioux, his band was intrigued by the Ghost Dance religion, which culminated in the massacre at Wounded Knee Creek in 1890.

Although Hump seems never to have become a true believer, he did lead his people in the Ghost Dance raids until early December of 1890. The US Army was alarmed by the Ghost Dance, and they sent emissaries to all of the major chiefs.

Captain Ezra Ewers – an old friend – was sent to speak with Hump. Ewers convinced Hump of the futility in armed resistance. At this point, Hump separated his band from the Dancers and led them to the Pine Ridge Agency.

As Hump was breaking camp, refugees from Sitting Bull’s group arrived and related how their leader had been killed during an arrest attempt. Sitting Bull’s people were eager to find allies as they sought revenge. Hump refused to help, and the refugees set out to join Big Foot near Wounded Knee Creek.

After the infamous massacre and subsequent events in 1890, Hump and several other Sioux chiefs went to Washington, D.C. They pleaded for fair treatment of their people.

Some of their requests were honored; however, the chiefs failed to gain concessions in other important areas. Reservation confinement continued, effectively ending the old way of life.

Hump died at Cherry Creek, South Dakota on the Cheyenne River Sioux Reservation in December 1908 at the age of 70. He is buried in the Episcopal Cemetery near there.

According to records provided by John Iron Moccasin’s family, Hump’s father was Iron Bull “TaTankaMaza”, and his mother was Ziti “Yellow Lodge”. Hump was born about 1848 when his father was 28 and his mother was 21.

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This photo was taken ca. 1879 by photographer by L.A. Huffman. The notation is that the photo is of Hump and his favorite wives. One of these women could well have been Beautiful Hail given that she appears to have had children in both 1882 and 1883 with Hump. He does look to be significantly older than the women.

Hump is shown with other Sioux leaders in this 1891 photograph.

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1891 Sioux Delegation LA-NA-DA-Kota

Front Row Seated; L to R: High Hawk, Fire Lightning, Little Wound, Two Strike, Young Man Afraid of His Horses, Spotted Elk (Oglala), Big Road; (2nd row standing) F.D. Lewis, He Dog, Spotted Horse, American Horse, Maj Gen Sword, Louis Shangreaux, Bat Pourier; (3rd row, standing) Dave Zephier, Hump, High Pipe, Fast Thunder, Rev. Charles Cook, and P.T. Johnson. Denver Public Library

In the 1900 federal census of the Cheyenne River Indian Reservation, district 48 in Sterling County, SD, the last census in which Hump was alive, he is shown on the census as having been married 20 years, born in April 1850 in Montana, with both of his parents born in the same place. He is a Ration Indian and he does not read, write or speak English. In the special inquiries section, he is listed as Sioux, his father as Sioux Cheyenne and his mother as Sioux. He is listed as entirely Native and in this census, is not listed as polygamous.

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His wife is listed on the next page as White Calf to whom he has been married for 20 years, so dating back to 1880. Of course, as suggested by the picture taken circa 1879 and the 1886 census in which Hump is married to 26 year old Beautiful Hail, White Calf was not his only wife. Given that Pretty Voice appeared in the census in 1876 with Beautiful Hail as a young child, I would presume that Beautiful Hail is Pretty Voice’s mother.

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Dora, who was born in 1891, is not shown living with Hump. I cannot find her elsewhere on the census. However, remember that Native people changed their names. Hope is listed as being born in July of 1889 in Montana.

In the 1917 Indian Census, Hope Hump is also listed as Dora, age 26, married to Willie Ward who was born in 1889. This shows us that Dora is Hope or Hoop Hump on the earlier census records.

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According to the 1900 census, Hope was born in July of 1889 in Montana, as were both of her parents. She does not read, write or speak English. She is 100% Sioux.

The following information was provided by http://files.usgwarchives.org/sd/ziebach/history/chap16-2.txt

Born in Montana in 1848 or 1850, Hump became a leader of the Cherry Creek band of Minneconjou Sioux.   In 1876 he fought in the Battle of the Rose bud against General George Crook and in the Battle of the Little Big Horn.

He later joined Sitting Bull’s band and other exiles in Canada.  Being considered American Indians, the exiles received no rations from the Canadian government. By 1881 the buffalo and other game were disappearing and the exiles returned to Fort Buford where they surrendered. They were taken to Fort Yates by steamboat. Later the Minneconjou under Hump and Fool Heart and the Sans Arc, led by Spotted Eagle and Circle Bear, were taken down the Missouri River to the Cheyenne River Agency, near their traditional camping grounds along the Cherry Creek and Cheyenne River.  They arrived at the Cheyenne River by May of 1882 and many of the Minneconjou settled near Cherry Creek, 50 miles west of the agency.

Hump and Big Foot became the most influential men on the Cheyenne River.  The Cherry Creek/Hump Band greatly opposed the land agreements of 1888 and 1889.  In 1890, the Ghost Dance found its greatest following in the Cherry Creek camps.

After Sitting Bull was killed on the Grand River, many of his followers fled south and camped a few miles above the junction of the Cherry Creek and Cheyenne River.  When the army at Fort Bennett moved to suppress the Ghost Dancing, Hump used his influence against the Ghost Dance. In the dead of winter he rode with two men from the garrison and two other scouts, 40 miles to persuade the Sitting Bull camp to surrender and move to Fort Bennett.  Those who did not surrender joined Spotted Elk, also known as Big Foot. When his band later fled toward Pine Ridge, they were met by the Army at Wounded Knee.

Hump was given 500 heifers for his service to the United States Government. These he turned loose, to share with his people. The heifers wandered near Leslie and many died of pinkeye.

Hump continued to work for his tribe until his death in 1908. He is buried in Cherry Creek.

HUMP

Told by John Hump

Hump (Thomas) was born in 1850 to Mashes His Nails/Iron Bull and Ziti/Mrs. Iron Bull (1827-1917) in Montana.

Hump’s brother, Little Crow, had been born in 1844. Hump’s sister, White Cow, married Fish (d. 1919) and had a son, James Fish (b. 1889) and a daughter. They lived on Rosebud.

Hump grew up in Montana. He had three or four wives, some of whom lived in Montana and were Crow.

While the Indians still roved in bands, he started to gather them together, to settle down and become ‘civilized’. Hump came down the Missouri River when the Army brought them to the Cheyenne River on boats. Their stock were driven over land.  Bertha Lyman Hump’s mother’s family came from Montana with Hump’s band.

Hump even joined the Army to work toward settling down. He was a scout from December of 1890 until June of 1891. He was discharged at Fort Bennett.

There were three Hump Flats. One east of Bridger, one by Iron Lightning and one across from Cherry Creek. All are so named because he lived on them. On the way to Montana for a visit, Hump camped with Iron Lightning on the Moreau River. At that time they chose their allotments. Iron Lightning community was later named for Iron Lightning after he moved there.

Hump had several wives. His son, by Good Voice/Good Woman, was Samuel Helper/ Stand by of Oglala, born in 1876.

Hump’s wife, White Calf/Bessie (d. 1915) was the mother of Pretty Voice/Nellie (b. 1882: Mrs. Alfred Ward); Important Woman/Sarah (b.1884: Mrs. Silas Yellow Owl); Spotted Bear who died in infancy; Dora (b.1891: Mrs. William Ward); Didn’t Drop/Nelson Hump, born in 1898 (no issue); William Miles Hump, born in 1900 and died in 1917 at Dupree, (no issue); and John Hump, born in 1904.

JOHN HUMP

John Hump was born at Cherry Creek, four years before his father’s death in 1908. Hump is buried at the Episcopal Cemetery in Cherry Creek.  John went to Carson Day School, Pierre Indian School and Rapid City Indian School.  In 1935 or 1936, he married Bertha Lyman, daughter of Ed Lyman. John transferred his heir ship lands from the Moreau River to Red Scaffold.

John and Bertha lived on the flat south of the (Cherry) creek, on her folks’ allotments. In 1954/1957 they moved north to their present home.  John went into the cattle business on the Rehab program. John and

Bertha’s sons, Duane and Darrell, now run the ranch.

Darrell is married to Alvina Runs After and Duane is married to Doris Halfred.

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The 1910 census taken at Cherry Creek station shows us that White Calf’s mother was Roan Hair, age 72, so born about 1838. She shows the birth of only one child.

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The special inquiries section tells us that she is Teton Sioux, full Native, married once, not polygamous, lived in an aboriginal dwelling and received her allotment in 1903.

Roan Hair is shown in the Indian census of the Cheyenne River Sioux in 1896 as the wife of Ragged, both age 56.

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Four years later, in 1901, they are shown again.

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Hump died on December 10, 1908 and is buried in the Episcopal Cemetery in Cherry Creek, SD.

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Hump’s Memorial at FindAGrave adds some additional information not found elsewhere.

Native American Chief. Sioux name “Etokeah.” Although very little is known about Hump’s early life, he eventually rose to become a Chief among the Miniconjou Sioux and was an active participant in the Red Cloud war. With Crazy Horse at the Rosebud Battle against George Crook, Hump led his Miniconjou Sioux, helping stop the column in their trek to meet Custer prior to the Little Big Horn. At the Little Big Horn, when the alarm was sounded, Hump jumped onto an unknown mount, and it which threw him to the ground. Hump rushed, mounted another horse and charged toward the soldiers. His horse was shot from under him and a bullet entered above the horse’s knee and went further into Hump’s hip. Hump was strained there due to the wound and did not participate in the main battle. Later, Hump went to Canada, and his band returned to the United States, the last of all the bands to return. On the reservation when other tribes had adopted white dress and housing, Hump’s band settled at Cherry Creek in South Dakota and maintained the old ways using lodges and traditional clothing. On the reservation when the authority of other chiefs wained, Hump continued to assert leadership over his band. Some said that Hump was feared by the whites even more than Sitting Bull. When the Ghost Dance religion surfaced among the Sioux, the military did not dare arrest Hump. Instead, they reassigned Captain Ezra Ewers, a trusted friend of the chief, to Fort Bennet in South Dakota. Ewers rode the 60 miles to Hump’s camp at Cherry Creek. Impressed with Ewer’s courage, Hump listened to his message and avoided the Ghost Dance religion. After the Wounded Knee Massacre, Hump along with other prominent Sioux went to Washington, DC pleading for a peaceful end to the tragedy. Interestingly enough, it was also Hump who taught the basic lessons of warfare to his better-known student, Crazy Horse. His grave is located on the west edge of the town of Cherry Creek.

This photo of Cherry Creek, probably in the early 1900s, shows both traditional teepees and more stationary buildings. This lends understanding to the special inquiries section of the census, and shows us what “fixed” dwellings look like as compared to “moveable.”

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The Hale Line

John’s mother was the daughter of Isabelle Ward and Robert Hale.

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South Dakota Marriage records show that Robert Clifford Hale, age 23, married Isabel Ward on May 3, 1946. Both lived in Cherry Creek, SD.

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Robert died on August 1, 2008. His photo and obituary are shown below.

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Death: Aug. 1, 2008 Sturgis Meade County South Dakota, USA
Robert “Bob” Clifford Hale, who lived in Cherry Creek, had the Lakota name Min A’ Kyan, which translates to Flies Over the Sea. While he may not have flown over the sea, he did ride the sea as a sailor in the U.S. Navy during World War II. Bob, at age 85, died Friday, Aug. 1, 2008, at the Fort Meade Veterans Hospital near Sturgis. He is survived by Larry (Delia) Hale, Theresa Hale, Herbert Hale and Cleo Hale, all of Cherry Creek, Martha (Erick) Hernandez of Chicago, Ill., Richard Hale of Rapid City, Connie (James) Bear Stops of Red Scaffold and Lavinia Hale-Eagle Chasing of Eagle Butte; grandchildren, Maude Hale, Denise and Richard Crow Ghost, Dawn Kills Crow, Angelic and Willard Demery of Cherry Creek, Amber and Alton Blacktail Deer Sr. of Manderson, Timothy Jr., Earl and Mary Iron Moccasin of Rosebud, Teno, Taun and Krista Bear Stops of Red Scaffold, Rhiana, Richard Jr. and Joshua Hale of Cherry Creek, Angel Prendergast and Aberham White Weasel of Rapid City, Maxine Flying By, Marsha Eagle Chasing of Eagle Butte, Sarah, Elizabeth, Mark and Posey Garter of Albuquerque, N.M., and Clinton and Kyle Harrison of Takini. Also surviving are his great great grandchildren, Morgan and Jasmine Hale, Eric Jarvis and Dewey Kills Crow, Kyra, Danieal and Alyssa Hayes, Adrienne and Royce Jr. Marrow Bone, Eric, Jarvis, Dewey, Drake and Autumn Kills Crow, Shantay Crow Ghost, Alton Blacktail Deer Jr., La’tia, Tyree and Lashae Bear Stops, D’Nica Ducheneaux, Tretyn Red Elk, Sage Bowker, Sarah Patryas, Jordan and Sierra Iron Moccasin, and Kleigh, Dawnelle and Deaconn Garter. Robert was preceded in death by his parents, Joseph and Ellen Hale; sisters, Claira Hale-Fritz, Myrtle Hale-Little Shield, Don’ta Black Tail Bear, Drazen Black Tail Bear, Mary Isabbella Kills Crow, Clifford Merle Hale; brothers, Martin and Wilson Hale; one daughter, Charmaine Hale Harrison; and his paternal grandparents. Funeral services for Robert were Saturday, Aug. 9, at the new Community Building in Cherry Creek. Ted Knife, Erick Hernandez and Elmer Zimmerman officiated. Hernandez read Matthew 7:7. Special music was provided by Buzzy Yellow Hawk, Daryl Whipple, the Tiospaye Singers, Michelle White Wolf and the Mennonite Singers. Harvey Eagle Horse played the Honor Song. Casketbearers were Bob’s grandsons, Joshua Hale, Taun Bear Stops, Timothy White Weasel Hr., Clinton Harrison, Posey Garter, Maris Reindall, Richard Hale Jr., Teno Bear Stops, Eric V. Kills Crow, Kyle Harrison, Mark Garter and Danny Hayes Sr. Honorary bears included all military veterans and all Bob’s other friends and relatives. Burial was at the UCC Cemetery in Cherry Creek under the direction of Oster Funeral home of Mobridge. Mobridge Tribune Wednesday, August 13, 2008

The US Department of Veteran’s Affairs BIRLS Death File lists Robert Hale’s birth and death dates and his service branch as Navy from July 24, 1942 to November 27, 1942.

The Social Security death index shows that Robert was born on Sept. 7, 1922.

I cannot find this family in 1920, 1930 or 1940 in the census, nor in the Indian census. It’s possible that the parents and siblings names are incorrect or nicknames.

Robert’s parents were given as Joseph Hale and Ellen in his obituary. John’s mother reports that Joseph Hale’s name was Joseph “Blows on Himself” and that this is the end of that line because they migrated from Canada on “the big trail.” I found nothing about this family at Ancestry or utilizing Google. It’s possible that the family was not living as a nuclear family as a recognizable unit.

The 1940 census shows a Joseph Hale, age 48, widowed, an Indian, as an inmate in the Davison County, South Dakota Jail, but we don’t know if this is the same Joseph Hale.  However, this is the only Joseph Hale in South Dakota, or for that matter, in that part of the country.

im-1940-census-hale

This Joseph was widowed, an Indian and born on an Indian Reservation, so it may well be the correct Joseph. It would be interesting to see if any court records still exist relative to this case.

I found scanty information on the following individuals from the obituary listing them as siblings of Robert Clifford Hale.

  • Claira Hale – married Elmer Fritz on February 27, 1962 , born about 1926.
  • Mytrle Hale – Myrtle Faye Hale married Theophil Little Shield and died in SD at age 65.
  • Don’ta Black Tail Bear – nothing
  • Drazen Black Tail Bear – nothing
  • Mary Isabella Hale Kills Crow – nothing
  • Clifford Merle Hale – nothing
  • Martin Hale – if the same Martin, died in 1935 of appendicitis, age 20.
  • Wilson Hale born about 1921 married Eunice Eagle Horse. He died in 1950 in Ziebach County. In the 1940 census he is living with the Straight Head family which would make sense if his mother was deceased and his father was in jail.

The Second Ward Line

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John Iron Moccasin’s grandmother on his mother’s side was Isabella Ward, born in 1925 or 1927.

The 1930 Federal census shows Isabella Ward, age 5, living with her parents in Ziebach County, SD. Her mother, Dora is listed as a full blood and her father, William, a mixed blood, all born in South Dakota and Sioux.

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Her father is listed as a farmer.

We’ve already met Dora (Hope) Hump, daughter of Chief Hump and probably White Calf and William Ward, son of Clarence “Roan Bear” Ward and Estella Dupris.

DNA Results

Now for the most exciting part – the DNA results. Do John’s DNA results bear out his genealogy?

John’s tribal card says that he is at least 15/16th Native. That is accurate, given that he is 1/16th French on both his mother and father’s sides, from the same ancestor.

In percentages, for autosomal DNA, that translates into 6.25% white and 93.75% Native.

When I’m working with descendants of tribes located east of the Mississippi, I understand that they are very likely heavily admixed with (primarily) European males, and significantly so prior to 1800 and in most cases, prior to 1700. However, the Sioux are somewhat different. Except for occasional traders and missionaries, they essentially escaped the widespread influence of Europeans until the 1800s. With few exceptions, I would not expect to find earlier mixing with Europeans, meaning English, French or Spanish, or Africans.

Because of the history of the Sioux tribe, the sheer number of Sioux across a wide geography, and the lack of early European admixture, John’s DNA represents an opportunity to obtain a genetic view of a people not significantly admixed.

Endogamy

We know from John’s family tree that he shares at least 3 ancestors and possibly 4 on both his mother’s and father’s side of the family. Those ancestors are 4 generations up the tree from John.

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In most cases, one’s great-great-grandparents would each contribute, on average, 6.25% of your DNA. In John’s case, he received a double dose of the DNA of each of those ancestors. If John received the exact same DNA from those ancestors, from both sides, he would still only have 6.25 % of their DNA. This is very unlikely, because normally siblings share part of their parent’s DNA, but not all of it. Conversely, it would be very unlikely for John to inherit none of the same DNA from that ancestor from both lines. Therefore, it’s most likely that instead of 6.25% of the DNA from that each ancestor who is found twice at 4 generations, he would carry about 9.38% of their DNA, or about half a generation closer than one would expect.

And that goes for all 3 common ancestors. We’re not sure which of Hump’s wives gave birth to which children, so this could also apply to Hump’s wife, a 4th ancestor.

Furthermore, these individuals in the tribes are likely already very heavily inter-married and related to each other, long before any records. There were only a limited number of people to select as mates, and all of those people also descended from the same ancestors, who were part of a very small foundation population that migrated from Asia some 10,000 to 25,000 years ago, depending on which model you subscribe to.

Therefore, endogamy and pedigree collapse where one shares common known ancestors would be a phenomenon that has occurred since the time of Anzick Child, and before.

John’s Tests

We tested John’s DNA at Family Tree DNA where his Y, mitochondrial and autosomal DNA was tested. John’s Y DNA shows us the deep ancestry of the White Weasel line. The mitochondrial shows us the deep ancestry of Dora (Hope or Hoop) Hump, daughter of Hump, presumably through wife, White Calf.

John’s autosomal DNA shows us an overall ethnicity view, plus matches to autosomal cousins. Let’s see what we have.

Autosomal Results

John’s myOrigins results show that he is roughly 17% European and the rest a combination of Native and Asian that together represents 84%.

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One of the aspects that I find most interesting is that the portion of Europe that shows a genetic link is Finland, not France where 6.25% of John’s paper trail ancestry is from.

Finland is particularly interesting in light of the result of the Clovis Anzick Child burial found in Montana that dates from about 12,500 years ago. We have the Anzick Child’s results in the Family Tree DNA data base, compliments of both Felix Immanuel and Family Tree DNA.

The Anzick child’s myOrigins results are shown below.

im-anzick-myorigins

The Anzick Child’s DNA ethnic results are very similar to John’s. Anzick Child matches the reference population for Finland at 11%, where John matches at 17%.

Furthermore, John Iron Moccasin is one of 110 people in the data base today that actually match the Anzick Child’s DNA at contemporary levels.

The match threshold at Family Tree DNA today is:

  • No minimum number of shared cM required, but if the cM total is less than 20, then at least one segment must be 9cM or larger.
  • If the longest block of shared DNA is greater than 9cM, the match will show regardless of total shared cM or the number of matching segments.

Lowering the match threshold to 3cM, we can see several small segments that match between John and the Anzick Child.

im-anzick-browser-5cm

I downloaded their common matching segments.

Chromosome Start Location End Location centiMorgans (cM) # of Matching SNPs
1 4282649 5290332 2.56 500
2 98863262 101324606 1.69 600
2 112439588 114460466 1.71 500
2 169362301 170609544 2.27 500
3 8964806 10632877 3.03 600
3 14230971 16121247 2.83 600
3 46655067 53174054 1.28 1000
4 12866760 14721835 1.85 500
5 78642903 80323930 1.64 500
5 158757557 162829228 3.82 1000
6 34609507 36812814 2.88 600
6 127839067 130105402 2 500
7 76597648 78055762 2.84 500
7 99319352 101758792 2.05 600
8 10455449 12975017 2.68 700
8 30301880 34206702 3.45 799
9 26018352 27374204 2.37 500
9 104470303 106854637 3.76 777
10 71258510 72644677 1.46 600
10 102514460 106018240 2.65 800
10 110936823 113553555 3.83 700
11 32265994 34530393 3.35 700
11 91619854 94670011 3.71 800
11 102068510 103853340 1.76 500
12 27332778 29165805 1.66 500
12 96875639 99784589 2.74 700
13 55048728 58723000 1.66 600
13 78707414 80906921 1.34 500
14 22564888 24752111 3.59 800
14 68418807 70225737 1.65 500
14 76767325 78038237 1.71 500
16 12528330 14375990 5.49 659
18 33126219 35069488 1.37 500
19 8284870 13355259 7.87 1278
20 45913972 47494552 3.17 500

Their largest matching segments are on chromosome 19 for 7.87 cM and on 16 for 5.49 cM.

The genetic connection between the Anzick Child and John Iron Moccasin is evident. John’s tribe is descended from the same people as the Anzick Child who was buried in present day Montana. John’s ancestors, Hump, Roan Hair and Follows were all born in Montana, and the Sioux homelands stretched across this entire region.

This begs the question of whether John is simply lucky to have inherited these segments, or if they are found widely in the Native, particularly Sioux, population as a whole.

To help answer this question, I looked at John’s closest 4 matches along with the Anzick Child in the chromosome browser, compared to John’s DNA.

im-anzick-match-compare

At 5cM there is no overlap with John’s closest matches and the Anzick Child, whose DNA is shown in green, above. However, dropping the threshold to 3, below, shows overlap with Thomas’s closest match on chromosome 19 at 4.98 cM and other chromosomes in smaller amounts. This would suggest that perhaps the DNA that is the same as the Anzick Child’s does not repose in the entire tribal population.

im-match-compare-3cm

Let’s take a look another way.

John and the Anzick Child at GedMatch

At GedMatch, John matches the Anzick Child on slightly different segments than at Family Tree DNA. It’s not unusual for different vendors to produce slightly different results. In this case, the match on chromosome 16 is absent altogether, and there are larger segment matches on chromosomes 8 and 14 using a 5cM and 500 SNP threshold.  Chromosome 22 shows a match not present at Family Tree DNA.

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I was curious to see how many people matched John on his segments shared with the Anzick Child.

John matches a total of 2119 people at GedMatch at 5cM and 500 SNPs.

John’s results for his two largest segments, chromosome 16 (at FTDNA) and 19 were different. Chromosome 16, the smaller match, was generally unremarkable, but his chromosome 19 was a different story, carrying many names and surnames that I recognize.

Let’s take a look at the triangulation tool and see what we find there. We are looking for anyone who triangulates with both John and Anzick Child. This tool reports every triangulated match in excess of 5cM.

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Using the triangulation tool, no one triangulates, meaning matches both John and the Anzick child, on either chromosome 16 or 19. This suggests that all of John’s matches showing are on the “other” chromosome and that this chromosome segment is fairly rare.

If one of John’s parents were to test, we could identify which of John’s parents was matching Anzick, so we would know which side of John’s family these individuals are matching on these segments, assuming these matches are not identical by chance.

Out of curiosity, I triangulated Anzick Child’s kit to see if there were any triangulated groups. There were, but none that included John.

At GedMatch, let’s use the “Are Your Parents Related?” utility. We know that John’s parents are related, but are any of the segments that came from both parents the same segment that is found in John’s Anzick match? The match threshold at GedMatch for this tool is 7cM and 700 SNPs, so the only segment that would qualify would be this segment on chromosome 19, shown above in green.

19 8284870 13355259 7.87 1278

The “Are Your Parents Related?” tool at GedMatch shows the following results.

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According to GedMatch, this segment of chromosome 19 was not contributed by both of John’s parents, so this portion of the Anzick DNA is not found universally in the entire Native population in that region.

One last look at John’s DNA by comparing to the Ancient group contributed at GedMatch shows no segments 4cM or above that match with any ancient specimen other than the Clovis (Anzick) Child, including no match to the Paleo Eskimo in Greenland from 4,000 years ago and no match to Kennewick Man. The tiny orange bars represent matching segments at 400 SNPs and 4cM.

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John’s Mitochondrial DNA

John’s mitochondrial DNA comes directly from his matrilineal line, meaning from his mother, her mother, her mother, on up the tree until you run out of direct line mothers.

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In this case, that person winds up being Hump’s wife. We think that person is probably  White Calf, but it could be one of Hump’s other wives. We just don’t know for sure given that Hump was polygamous.

Mitochondrial DNA is passed intact in each generation, doesn’t get combined with the father’s DNA so it’s a direct line back in time.

Johns’s mitochondrial haplogroup is clearly Native, C4c1.

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Haplogroup C4c1 was originally reported in the Suswap by Ripan Malhi; in the Chippewa Creek and in Jasper House, Alberta Canada, in 2015 by Roberta Estes from the American Indian project.

At the HVR1 level, John has 62 exact matches, but he has no matches at the HVR2 or full sequence levels. This means that of the people who have tested at that level, he has more than 4 differences at the full sequence level. Translated, this means they don’t share common ancestors in hundreds to thousands of years.

Only 8 of John’s HVR1 matches have tested at the full sequence level, unfortunately.

Of those, the earliest ancestors are Spanish, indicating that they are probably from either the American southwest, or further south, and their haplogroup C ancestor was eventually associated with the Spanish. One is from New Mexico. One is from Michigan.

Few of John’s matches have entered the location of their most distant ancestor, but those who have provided that information are shown below at the HVR1 level, understanding that a common ancestor at that level could predate the migration into the Americas.

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Utilizing the information provided through the Genographic project, we find the following information about haplogroup C4c1.

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This provides very interesting geographic distribution information, but it also begs the question of how haplogroup C4c1 was found in Germany or Sweden. Of course, we are relying on participant-reported information and it’s certainly possible that two individuals misunderstood the directions. It’s also possible that one or both are legitimate. I have wondered for a long time about a link between the northern Scandinavian populations, especially subarctic, and the Native subarctic populations in North America.

According to Dr. Doron Behar in the supplement to his paper titled, “A Copernican” Reassessment of the Human Mitochondrial DNA Tree from its Root,” haplogroup C4c1 was born about 10,095 years ago with a standard deviation of 4550 years, meaning the range of time in which C4c1 was born in likely 5,545 to 14,645 years ago. Clearly, there is enough latitude in this date range for some C4c1 to be found in either Asia or Europe, and C4c1 to be found in the Americas as well. If this is indeed the case, one would expect for the variants of C4c1 found on the differing continents to contain a significant difference in mutations, exceeding the 4 mutations allowed for genealogical matching purposes at Family Tree DNA.

To date, there has been no ancient DNA recovered bearing this haplogroup.

Other Mitochondrial Results

Individuals descending from several of John’s maternal lines would be perfect candidates to test for the mitochondrial DNA of those lines. One must descend from these women through all females to the current generation:

  • Follows
  • Esther Ward – Nellie “Pretty Voice” – Beautiful Hail or White Calf
  • Ellen (wife of Joseph Hale)

Testing a female descended through Pretty Voice, mother of Esther Ward, would determine whether or not White Calf was the mother of Pretty Voice, or if it was another woman, probably Beautiful Hail.

John’s Y DNA

John inherited his Y DNA chromosome from Charley White Weasel.

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John’s Y haplogroup is Q-M242, a Native haplogroup.

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John tested to the 67 marker level, but has no matches at 67 markers. At 12, 25 and 37 markers, he matches a gentleman whose ancestor was from Fort Thomson, SD who also tested at 67 markers. That is John’s only match, so apparently John carries some unusual mutations in his Y DNA as well that are probably isolated to people from the Sioux tribe or their ancestors in the past a few hundred to thousands of years.

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On the map above, John’s match is shown and on the map below, John’s white balloon is shown where he was born in relation to that of his red balloon match.

im-y-matches-map-2

To obtain additional information about John’s Y DNA haplogroup, the Big Y test would need to be run on his sample. By running the Big Y, we could obtain a more granular haplogroup, meaning further down the tree, and we could also see who matches him more distantly, meaning further back in time. That information could well provide us with information indicating which groups of Native people John is most closely related to. That suggests a migration route or pathway and tells us about social interactions at some level hundreds to thousands of years in the past.

Anzick Child’s Y DNA haplogroup is Q-L54, a subgroup of Q-M242, shown on the haplotree below. You can also see that many subgroups below L54 have been discovered.

im-hap-q-tree

I strongly suspect that John’s haplogroup would be Q-L54 or a subgroup further downstream. I’m betting on a subgroup, meaning that mutations have occurred in John’s line that define a newer, younger haplogroup since the time that Anzick Child and John shared a common ancestor.

Other Y Line Results

I was hopeful that I would find results for John’s Ward or Hale line in the projects at Family Tree DNA, but I did not. I checked in the American Indian project for Hump, with the hope that one of his descendants has tested as well, but did not find that Hump is yet represented in the data base. Of course, anyone paternally descended from Hump’s father, Iron Bull or his father, Black Buffalo would carry the same Y DNA.

If anyone descends from these direct Y lines, please do let us know.

Summary

What we have been able to discover about John’s ancestry both through traditional genealogy and genetic genealogy has been both amazing and fascinating.

John now knows that he is connected to the Anzick Child, the Ancient One. John’s ancestors and Anzick’s were one and the same. Some 12,500 years later, John was born on the same land where his ancestors have literally lived “forever.”

Anzick has given John a wonderful gift, and John has given that gift to the rest of us. We continue to learn through both John and Anzick’s contributions. Thank you to both.

What’s Next?

I would very much like to upgrade John’s Y DNA to 111 markers and order a Big Y test while the holiday sale is in effect. If you would like to contribute to these tests of discovery, please donate to the American Indian project general fund at this link. If we raise more than we need for John’s tests, we have implemented an application process for other Native people. Every donation helps, and helps to build our knowledge base – so please contribute if you can.

Acknowledgements

My gratitude to the following people:

John Iron Moccasin for testing, providing family information and allowing us to work with and publish his results.

John’s mother, Martha Hale, for providing the original genealogical information, below.

im-original-pedigree

Johns’ friend, Pam, for bringing us this opportunity.

John’s wife, Carolyn, for coordinating information.

Family Tree DNA for testing and facilitating the Ancient DNA Project, the American Indian Project and various Native American haplogroup projects.

nat-geo-logoThe National Geographic Society Genographic Project for providing data base access to the project administrators of the American Indian Project as Affiliate Researchers

Project members and others for contributions to facilitate John’s testing.

My American Indian project co-administrators, Marie Rundquist and Dr. David Pike for their never-failing support.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

Concepts – Sorting Spreadsheets for Autosomal DNA

This article covers both sorting in Excel and how to identify an overlapping segment, and what that means to you as a genetic genealogist.

I swore I wasn’t going to teach Excel, but there have been so many questions about sorting Excel spreadsheets that I am going to a very basic “how to sort and not hurt yourself” article. This does NOT replace actually understanding how to use Excel, but it will at least get you through the knothole of sorting for genetic genealogy.

I wrote more about sorting and filtering in the concepts article about assigning parental sides.

There are some advanced ways to accomplish the same thing, and I’m not discussing those. If you already know how to use Excel those are fine, but this article provides the basics for those who don’t.

Sorting

I am going to use, as an example, my matches to only a few people which gives us enough information to sort, but isn’t overwhelming.

When you download your results from Family Tree DNA, your spreadsheet will be in match name order, like the spreadsheet below.

SS Raw

I want you to notice that while the primary order is by match, there is a secondary order too (chromosome), and a third (start location) and fourth (end location) as well.

Within each match, the order is by chromosome, and then by start and end location.

What this means that you can look at Alice and see that chromosome 1 is first, and that the lowest value start location is shown first within chromosome order.

That’s not the order you’ll likely be working with all the time, so let’s take a look at how to sort the spreadsheet in a different way.

The row highlighted in red contain column headers.

SS column headers

When you sort an individual column you will select the header for that column, shown below, if you’re going to sort the Matching SNPs column.

SS Column select

The cell on your spreadsheet won’t be red, but I’ve colored it red here so you can see that I’m selecting this column header and only this column header.

When you select a column header, you put the cursor on that cell and click once.

SS column select 2

The cell you’ve selected will be bordered in black.  A screen shot of my spreadsheet is shown above.

I want you to watch what happens to these two rows colored green when I sort in Matching SNP order.

SS rows green

At this point, you will click on the sort and filter button on the upper right hand side of the toolbar.

SS sort dropdown

Here’s a closeup.

SS sort dropdown closeup

Selecting the “Sort A to Z” option sorts the contents of the entire spreadsheet in Matching SNP order, smallest to largest, because that’s the column header and sort option combination you selected. I use lowest to highest (A-Z) but you can also sort in reverse order, highest to lowest (Z-A) but that isn’t terribly useful for what we will be doing.

SS SNP column sorted

Notice that all of the rows are sorted into smallest to larger order by the Matching SNP column. So while the two green rows were originally together, now the rows all appear in order by the Matching SNPs column values.

The first green row match to Alice on chromosome 3 with 1300 cMs falls between the SNP value of 850 and 1458.  The second green row with a value of 2000 falls between 1638 and 2355.  This is exactly as it should be.  The contents of the entire spreadsheet are sorted by the values in the Matching SNPs column.

The statement “sorts the contents of the entire spreadsheet” is very important, because if you perform this task incorrectly, you will bollux up your entire spreadsheet, as in irrecoverably and forever.  What follows is an example of what NOT TO DO.

DO NOT DO THIS

DO NOT, and I repeat, DO NOT select the entire column to sort.

SS - Do Not Sort

This is an example of WHAT NOT TO DO.

If you select the entire column, as shown above, then sort, here’s what happens.

SS example bad sort

Notice that the green rows are now split apart – in other words they no longer form a row from left to right. That means that ONLY the data in the Matching cM column was sorted, but not rest of the data which is still in the same location on the spreadsheet as it was before the sort. Therefore, Alice’s green row Matching cM value of 1300 is no longer with Alice, since only the data in the Matching SNPs column was sorted. Now Alice’s 1300 cMs connected to Stacy’s red row on chromosome 4. Alice now has 500 SNPs instead, which as you can see, clearly isn’t accurate.

This is what I meant by selecting the entire column instead of just the header will forever ruin your data. If you do this, there is no recovery, unless you JUST did it, SS undo
realize the error, and can selecte the blue backarrow on the top of the toolbar on the left to “undo” your action. If you’re beyond that, the only recovery is to download your data again, or move to a backup if you have one.

What’s even worse if you do this and don’t realize it, so you’re working with incorrect data trying to find overlapping segments.  Of course, everything will be wrong.  I periodically do a sanity check and look at a couple people in the chromosome browser just to make sure that everything is as it should be on my spreadsheet and I haven’t done something like this.

To Sort Correctly – DO This

To use this spreadsheet effectively for genetic genealogy, we need the spreadsheet to be sorted in this viewing order:

  • Chromosome number
  • Start location
  • End location

In other words, we need the spreadsheet to look like this with all of the green cells remaining in their row with their match:

SS example good sort

You’ll notice that all matches on each chromosome are grouped together, with the smallest start location first, as illustrated by the red groupings of chromsomes 1 and 6. I do realize these are small segments, but the process is the same for large or small segments, so for our sorting example, just ignore any genealogical relevance associated with segment size.

You will be looking for overlapping segments. Notice that you have to be cognizatnt of the end location. In the case of chromsome 1, above, there are no overlapping segments for the two chromsome one matches, so they can’t match each other on this segment.

However, on chromsome 6, we have a different situation. Stacy’s segment match with me is quite long, 104cM. Stacy’s segment overlaps with everyone else’s on chromsone 6 that matches to me, either fully or part way. She matches Alice on all of the segments fully except for the last one. Stacy’s match to me ends at 108,000,000. Alice’s last segment matches to me from 107,779,220 which is included in Stacy’s match, but Alice’s match extends beyond Stacys, to 110,175,307.

Keep in mind that we don’t know at this point whether or not Stacy and Alice are from my mother or father’s side, based on matching. In other words, to draw any conclusions, we also have to know if Stacy and Alice match each other on this segment which we can’t tell from this spreadsheet.

Because I have access to Stacy’s account, I can indeed tell you that Stacy and Alice do not match each other on this segment, so they would be from different sides of my family tree. Stacy is a known relative from my father’s side and Alice does match my mother as well, so we now know that Stacy and Alice don’t match each other.

If you don’t have access to the accounts to see if your matches match each other, two tools at Family Tree DNA are partial substitutes.

  • The ICW tool tells you if two of your matches match each other, just not on which segments.
  • The maternal/paternal Family Matching tool, if you have connected the DNA of relatives who have tested, tell you which side your matches are from, maternal or paternal.

You can read about how to use those tools here.

If there are multiple matches with the smallest start location then they will be in order by the smallest end location first, shown in the yellow cells.

Sort Order

The sort order is exactly the opposite of the viewing order. If you want to SEE the data in this order:

  • Chromosome
  • Start
  • End

Then you must sort in this order:

  • End
  • Start
  • Chromosome

The last column you sort will be the primary viewing order.

Let’s look at our spreadsheet utilizing these three steps, in order.

Step 1 – First Sort

Selecting End Location to sort:

SS sort end location

After sorting by end location, below.

SS end location sorted

You will notice that all of the data is now in order by the values in the End Location column – smallest at the top, largest at the bottom.

The data in the other columns is not in any particular order at all.

Step 2 – Second Sort

Now selecting Start Location to sort that column in order, shown below.

SS sort by start location

Having sorted by Start Location, below:

SS sorted by start location

You will notice that now all of the data is sorted by start location. In the case where there is a common start location between two rows, highlighted in red, the end row with the lower end location will show first, noted in yellow, because you sorted first by end location in smallest to largest order.

Step 3 – Third Sort

Last, you’ll select the Chromosome column header to sort in chromosome order.

Sort by chromosome

Below, the result of sorting the third time in chromsome order.  After sorting, I bordered all segments on the same chromosome.

Sorted by chromosome

You can see that the entire spreadsheet is grouped by chromsome, and within chromsome number, the Start Location is grouped smallest to largest. If there are multiple people with the same start location, then the End Location comes into play, with the smallest end location listed first, as shown in the red and yellow rows.

If you want to sort your spreadsheet in another order for some reason, you can do so using the same methodology. Once you understand about sorting spreadsheets, you understand about sorting all spreadsheets.

Now, you’re ready to look for your overlapping segments.

What is an Overlap?

An overlap is two segments of your matches that are partially or completely overlapping each other.  When you have overlapping segments, assuming they are of decent size, that indicates that the two people who match you on your spreadsheet potentially match each other too.  Remember, there are three matching possibilities:

  • Your matches will either match each other, in addition to you, because you and both of them share a common ancestor or…
  • They both match you, but they won’t match each other because one is from your mother’s side and one is from your father’s side or…
  • One or both are identical by chance.  In you need a refresher on what identical by chance, descent and population mean, click here.

Ss no overlap

In this first example, above, there is no overlap between these two people on chromosome 17.  One begins at 31,000,000 and ends at 36,000,000 while the second person’s match with you doesn’t begin until 40,000,000, which is clearly beyond the end of 36,000,000, so there is no possibility of overlaps between these two individuals.  In other words, they cannot match each other on these segments.  However, clearly they both match you because they are both on your matching spreadsheet.

SS overlap 1

In the example above, the overlapping portion of the segment is from 38,000,000 – 40,000,000.  The second person’s match with you extends to 53,000,000, but the area between 40,000,000 and 53,000,000 does not overlap.

SS overlap 2

In the example above, the start number is lower for the top row than the second row, so the overlapping area is still from 38,000,000 – 40,000,000, because the matches don’t match from 36,000,000 to 38,000,000.

SS overlap 3

Occasionally, you have an overlap that is fairly miniscule, which I generally ignore unless they are in a group that has a larger overlap that overlaps or covers both smaller matches, as in the example above. You can see that our red and yellow rows have a very small overlap from 39,500,000 – 40,000,000. However, the top row includes the entire areas of both red and yellow rows, reaching from 33,000,000 to 55,000,000 which begins before either red/yellow row and ends after both red/yellow rows.  So either all 3 individuals will match each other, indicating a common ancestor, or the top row will match one of the red/yellow rows and not the other.

Combining Spreadsheets From Different Sources

The good news is that you can download your matches into a spreadsheet format from  23andMe, Family Tree DNA and GedMatch, but you do need to understand something about the basics of sorting and how to stay out of spreadsheet trouble. I am careful about combining spreadsheets sources for a couple of reasons.

  • First, the formatting is not exactly the same, so you may need to move columns to be in the correct order for your spreadsheet before actually combining them.
  • Second, there may be overlapping people between 23andMe, Family Tree DNA and GedMatch. You’ll need to figure out how you want to deal with that, especially on an ongoing basis when you need to add to or update your spreadsheet without overwriting or eliminating your matching work and notes relative to common ancestors and ancestral lines in the columns you’ll be adding.

I always make a backup file with a date name in the file name before doing combinations, and sometimes before sorting as well.

Learning Excel

If you want to learn more about how to use Excel, here are some additional resources to utilize.

I found some training videos for Excel including “Twenty with Tessa, Tips and Suggestions for Spreadsheets” which is focused on using spreadsheets with one name studies and genetic genealogy, but the principles are the same.  https://www.youtube.com/watch?v=Ll_cfhOZTl0&feature=youtu.be

When discussing this online, one person mentioned that they joined www.lynda.com and took the basic Excel class which she found very useful.

Kitty Cooper has instructions on her blog for how to make a matches spreadsheet as well.

www.DNAadoption.com has some good courses.  Their DNA for beginners covers using spreadsheets and is not just for adoptees!

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research

Additional Relatives Added to Phased Family Matches at Family Tree DNA

Family Tree DNA has been rolling out updates and upgrades fast and furious.

On July 7th, Family Tree DNA released Phased Family Matches which included phasing to people linked to your tree who have DNA tested who are related to you.  These phased matches allow Family Tree DNA to assign matches to maternal or paternal buckets, or both.  The people that could be utilized for this phased matching were as follows:

  • Parent(s)
  • Aunts
  • Uncles
  • First Cousins
  • Grandparents

Of course, because everyone wants the most people possible in their assigned parental buckets, the first clamor was for the addition of:

  • Half siblings
  • Half “other relatives” such as aunts, uncles, first cousins, etc.
  • Second Cousins
  • Third Cousins

Family Tree DNA said that there would be additional new developments shortly, and exactly 20 days later, they quietly rolled updated capabilities that includes matching to…..you guess it….all of the above, plus more, including:

  • Great-great-grandparents
  • Great-grandparents
  • Grand uncles
  • Grand aunts
  • Great-grandaunts
  • Great-granduncles

I’m certainly envious of anyone who can test their great-grandmother – although my grandchildren have their great-grandmother, grandmother and both parents in the system.

In my case, before this change, the only relative that I had in the system that originally qualified was my mother. I was very excited to have people in my maternal bucket and was wishing for people in my paternal bucket. I do have several cousins who have tested on my paternal side, but none as close as 1st cousins.

Imagine my delight when I signed on to my account and discovered 359 individuals in my paternal bucket and one in both, in addition to my 256 maternal phased matches.

Both Buckets

These 359 phased paternal matches come from the combination of the following 8 individuals that have tested and I had previouisly linked to me in my tree:

  • Half sister’s granddaughter
  • Two first cousins once removed
  • One first cousin twice removed
  • One second cousin
  • One second cousin once removed
  • Two third cousins

Of course, now I’m searching through my DNA matches to see if I have anyone else who qualifies that has tested.

And I’m thinking about any other cousins that would benefit my phased parental bucket assignments if I were to be able to convince them to test.

I unlinked and relinked a few people to see how many people were added to the buckets because of them.

The second cousin once removed added 12 new people. Yet, one of the third cousins added 82, so you really never know. Some of the people who might have been added to a bucket by the second cousin may have already been added to the parental bucket by an earlier match.

Regardless, the more people linked to your tree from third cousins closer, the better your chances for having people assigned to maternal and paternal sides of your tree, even without having your parents.

Keep linking people in your tree when you know where and how they connect to you – regardless of where they are located in your tree.  You never know how that may benefit you – which morning you may wake up and find additional information or more people in your buckets.  What a great surprise!!!

This is a pretty amazing feat if you think about it, given that just a few years ago autosomal testing wasn’t available at all, and even today, no other vendor does phased matching, assigning individuals to maternal or paternal buckets utilizing parents and other relatives when parents aren’t available.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research