Charlemagne (742/748-814), Holy Roman Emperor, 52 Ancestors #103

Charlemagne statue

“Charlemagne Agostino Cornacchini Vatican 2” by Photo: Myrabella / Wikimedia Commons. Licensed under CC BY-SA 3.0 via Commons

Above, a 1725 statue representing Charlemagne housed in St. Peter’s Basilica in Rome.

Wow, do I ever have a lot of cousins.  According to Graham Coop, everyone in Europe today is descended from Charlemagne.  Which either means I’m special and so is everyone else, or we’re all just normal.  National Geographic wrote an article about the results of the Coop study in more easily readable verbiage, here.  The Coop team also wrote a nontechnical FAQ, here.

In 2002, Steven Olson wrote this verbiage in the Atlantic magazine about the work of statistician Joseph Chang:

The most recent common ancestor of every European today (except for recent immigrants to the Continent) was someone who lived in Europe in the surprisingly recent past—only about 600 years ago. In other words, all Europeans alive today have among their ancestors the same man or woman who lived around 1400. Before that date, according to Chang’s model, the number of ancestors common to all Europeans today increased, until, about a thousand years ago, a peculiar situation prevailed: 20 percent of the adult Europeans alive in 1000 would turn out to be the ancestors of no one living today (that is, they had no children or all their descendants eventually died childless); each of the remaining 80 percent would turn out to be a direct ancestor of every European living today.

Now, granted, Charlemagne was indeed prolific, siring 18 known children.  I guess I’m just lucky in that I descend from two known children, one who was the King of Italy and one who was the King of France.  I must tell you, all this king stuff sounds very surreal to me.

Charlemagne chart crop

Now, the bad news.  In spite of all of these children, it appears that none of Charlemagne’s male lines survived more than a dozen or so generations.  In generation 8, only two descendants produced a male child, so that severely limited the possibilities for his Y DNA to reproduce – and it didn’t.  It apparently died out in 1122 with the death of the last male in an illegitimate line through Charlemagne’s son, Pepin of Italy.  This means that today, we don’t know what Charlemagne’s Y DNA looked like.  That’s very disappointing.

By the same token, Charlemagne could not pass on his own mtDNA.  To find that information, we’d have to find a female sibling or someone from his mother’s line who descends from a matrilineal female through all females to the current generation.  We don’t have that either.

And as for autosomal DNA…well, we have two problems.  The first is that Charlemagne is so far back in my or anyone else’s lineage – between 40 and 49 generations roughly – that we would carry very little if any of his DNA today…and there is no way to assure that we don’t have other common lines too.  In fact, at that distant point, it far more likely that we do share other common lines that we don’t, especially given what Graham Coop’s paper indicates.

So, Charlemagne’s autosomal DNA hasn’t been identified either.  Short of digging him up, I’m doubting we’ll know much more.  Actually, poor Charlemagne has been dug up, several times in fact, and parts of him given away as religious relics.  In 1988 scientists tried to reassemble him, and their report was delivered 26 years later, without DNA unfortunately, but confirming as best they can that the remains they have, shown in the photo below, are indeed Charlemagne.  I must say, it’s a very odd feeling to look at the bones of my ancestor, reassembled during the study.  In fact, I don’t think I’ve ever seen the bones or even one bone of any of my ancestors before now.  I’m not exactly sure what I think of this.

Charlemagne bones

I’m striking out here genetically, although I’m hopeful for the future since his bones are already exhumed.  To begin with, DNA could tell us if all of those bones are really from the same person.  If they are, or most of them are from the same person, it’s more likely to be Charlemagne.  We could potentially tell when and where this skeletal person lived from isotope testing as well, which could help us confirm or eliminate the possibility that these skeletal remains are Charlemagne.  Y and mitochondrial DNA would tell us a lot about his ancestors, and therefore, ours too.  I hope this avenue is being pursued.

Let’s see what we can discover about Charlemagne outside of genetic genealogy.

Charlemagne’s Birth and Ascent to Power

Charlemagne was born on April 2, in either 742, 747 or 748 and died on January 28, 814.  No, those aren’t typos, they are genuinely three digit years.  It’s hard for me to come to grips with the fact that I have ancestors that I can identify that were born 1270 years ago.

Charlemagne’s father was Pepin the Short and his mother, Bertrada of Laon.  He was of the Carolingian dynasty and was, of course, Catholic. In fact, Charlemagne was DEVOUTLY Catholic, which plays a big part in the decisions he made in his lifetime.  Either that, or he used his religious fervor as an excuse for his invasions of other non-Christian domains.  It’s much easier to track the history of what he did rather than to discern his motivations.

The Carolingian dynasty was a Frankish noble family with origins in the Arnulfing and Pippinid clans of the 7th century AD.

Charlemagne Carolingian chart

This Carolingian family tree, above, dates from the Chronicon Universale of Ekkehard of Aura from the 12th century, but reflects earlier generations.  The Carolingian empire came to a close not long after after Charlemagne’s rule, about 888.

The name “Carolingian” was in Medieval Latin, karolingi, an altered form of an unattested Old High German karling or kerling, meaning “descendant of Charles.”

Charlemagne’s birth year remains uncertain.  The most likely year of Charlemagne’s birth is reconstructed from several sources. The date of 742, calculated from Einhard’s date of death of January 814 at age 72, predates the marriage of his parents in 744.  Einhard was a Frankish scholar and servant of Charlemagne (and his son) who also served as Charlemagne’s biographer – thankfully.

The year given in the Annales Petaviani, a year by year history of the Carolingina empire, 747, would be more likely, except that it contradicts Einhard and a few other sources in making Charlemagne seventy years old at his death. The month and day of April 2 is established by a calendar from Lorsch Abbey.

In 747, that day fell on Easter, a coincidence that likely would have been remarked upon by chroniclers but was not. If Easter was being used as the beginning of the calendar year, then April 2, 747 could have been, by modern reckoning, April 2, 748 (not on Easter). The date favored by the preponderance of evidence is April 2, 742, based on Charlemagne’s being a septuagenarian at the time of his death. This date would appear to suggest that Charlemagne was born illegitimately, which is not mentioned by Einhard.

Einhard said the following:

It would be folly, I think, to write a word concerning Charles’ birth and infancy, or even his boyhood, for nothing has ever been written on the subject, and there is no one alive now who can give information on it. Accordingly, I determined to pass that by as unknown, and to proceed at once to treat of his character, his deeds, and such other facts of his life as are worth telling and setting forth, and shall first give an account of his deeds at home and abroad, then of his character and pursuits, and lastly of his administration and death, omitting nothing worth knowing or necessary to know.

We also don’t know where Charlemagne was born, but Aachen in today’s Germany has been suggested.

Charlemagne became king in 768 following the death of his father. He was initially co-ruler with his brother Carloman I. Carloman’s sudden death in 771 under unexplained circumstances, left Charlemagne as the undisputed ruler of the Frankish Kingdom. Charlemagne continued his father’s policy towards the papacy and became its protector, removing the Lombards from power in northern Italy, and leading an incursion into Muslim Spain. He also campaigned against the Saxons to his east, Christianizing them upon penalty of death, leading to events such as the Massacre of Verden in which 4500 captive Saxons were slaughtered.  Charlemagne was not always a kind man – but history remembers him as an exceedingly effective ruler.

Charlemagne and Pipin the Hunchback

Above, Charlemagne on the left and his son, Pepin the Hunchback, who revolted against his father.  Pepin the Hunchback was subsequently censured and exiled to a monastery instead of put to death after his father commuted his death sentence.

We don’t know how much this drawing actually looks like Charlemagne since it is a 10th century copy of a lost original from about 830.

In this next drawing, Charlemagne instructs his son, Louis the Pious.

Charlemagne and Louis the Pious

Charlemagne was also known as both Charles the Great (Carolus Magnus) or Charles the First.  He became the King of the Franks beginning in 768 with the death of his father and King of Italy beginning in 774.  He was crowned Holy Roman Emperor (Imperator Augustus) in the now demolished Old St. Peter’s Basilica in Rome (shown sketched below between 1483-1506) on Christmas Day in the year 800 and he ruled in that position until his death.

Charlemagne Old St. Peters

This fresco below shows a cutaway view of the Old St. Peter’s from the 4th century, so it looked much like it would have to Charlemagne.  This basilica is built over the location believed to be the burial site of St. Peter.

Charlemagne St Peters cutaway

Today, a new St. Peter’s Basilica stands on this site, the dome visible from the Ponte Umberto I on the Tiber River, below.

"Vatican City at Large" by Sébastien Bertrand from Paris, France - Flickr. Licensed under CC BY 2.0 via Commons

“Vatican City at Large” by Sébastien Bertrand from Paris, France – Flickr. Licensed under CC BY 2.0 via Commons

Although he already ruled both Italy and France, becoming the Holy Roman Emperor bestowed upon him divine grace and a Godly legitimacy sanctioned by the Pope.

This painting from 1516-1517 by Raphael by depicts Charlemagne’s coronation.

"Raphael Charlemagne" by Raphael - Licensed under Public Domain via Commons

“Raphael Charlemagne” by Raphael – Licensed under Public Domain via Commons

Charlemagne’s Rule

Charlemagne map 800

Called the “Father of Europe” (pater Europae), Charlemagne united most of Western Europe for the first time since the Roman Empire and laid the foundations for both modern France and Germany. His rule spurred the Carolingian Renaissance, a period of energetic cultural and intellectual activity within the Church. Both the French and German monarchies considered their kingdoms to be descendants of Charlemagne’s empire.

Charlemagne didn’t seem to stay home much.  In fact, this military and political history reads like a soap opera, with intrigue, betrayals, a brother who died in unexplained circumstances, but apparently of natural causes, invasions, rebellions and saving an injured Pope.  His life was assuredly interesting and it’s nothing short of amazing that he managed to live past 70 and did not die on the battlefield.

Charlemagne was engaged in almost constant warfare throughout his reign, often at the head of his elite scara bodyguard squadrons, with his legendary sword Joyeuse in hand.

Charlemagne sword

“Épée de charlemagne” by Chatsam – Own work. Licensed under CC BY-SA 3.0 via Commons

Joyeuse is stunningly beautiful and is on display in the Louvre today.

"Epée Joyeuse" by Siren-Com - Own work. Licensed under CC BY-SA 3.0 via Commons

“Epée Joyeuse” by Siren-Com – Own work. Licensed under CC BY-SA 3.0 via Commons

It just kills me that I have been in this building with this sword but didn’t know at that time that Charlemagne was my ancestor.

The 11th century “Song of Roland” describes the sword:

[Charlemagne] was wearing his fine white coat of mail and his helmet with gold-studded stones; by his side hung Joyeuse, and never was there a sword to match it; its colour changed thirty times a day.

Some seven hundred years later, Bulfinch’s Mythology described Charlemagne using Joyeuse to behead the Saracen commander Corsuble as well as to knight his comrade Ogier the Dane.

The town of Joyeuse, in Ardèche, is supposedly named after the sword.  Joyeuse was allegedly lost in a battle and retrieved by one of the knights of Charlemagne; to thank him, Charlemagne granted him an appanage (estate) named Joyeuse.

Today, Joyeuse is used as the French coronation sword.

Charlemagne’s Additions to His Empire

Charlemagne spent his entire life increasing the size and power of his empire, some of which was done under the banner of expanding Christianity to the Muslim world and to the pagan Saxons as well.

The map below shows the land that Charlemagne added to the Frankish Kingdom.

"Frankish Empire 481 to 814-en" by Sémhur - Own work, from Image:Frankish empire.jpg, itself from File:Growth of Frankish Power, 481-814.jpg, from the Historical Atlas by William R. Shepherd (Shepherd, William. Historical Atlas. New York: Henry Holt and Company, 1911.). Licensed under CC BY-SA 3.0 via Commons

“Frankish Empire 481 to 814-en” by Sémhur – Own work, from Image:Frankish empire.jpg, itself from File:Growth of Frankish Power, 481-814.jpg, from the Historical Atlas by William R. Shepherd (Shepherd, William. Historical Atlas. New York: Henry Holt and Company, 1911.). Licensed under CC BY-SA 3.0 via Commons

Charlemagne’s reign of Aquitaine began in 768 with the death of his father, although it was initially a joint reign with his brother, Carloman, with whom he had, at best, lukewarm relations fostered by his mother.

It didn’t take long after Charlemagne’s father death for trouble to brew.

In 769, a small uprising in the Basque region was subdued, but that region was unstable for years, a constant thorn in Charlemagne’s side.  Finally, in 781, Charlemagne proclaimed his son, Louis the Pious, then a young child, the first Frankish king of the area, assuring loyalty and displacing those whose loyalty he had reason to doubt.

In 770 Charlemagne married the daughter, Desiderada, of a Lombard King as a political move to form an alliance with her father and in doing so, surround  brother Carloman with Charlemagne’s allies.  However, by the end of 771, Carloman was dead and Charlemagne no longer needed the marriage with Desiderada, so he repudiated her and set her aside to marry 13 year old Hildegard.

In rejecting Desiderada, Charlemagne incurred the wrath of her father, the Italian King Desiderius.  Carloman’s widow and children took refuge in King Desiderius’ court at Pavia for protection.

This drawing is of the Carolingian cavalry from that timeframe.

"Karolingische-reiterei-st-gallen-stiftsbibliothek 1-330x400". Licensed under Public Domain via Commons

“Karolingische-reiterei-st-gallen-stiftsbibliothek 1-330×400”. Licensed under Public Domain via Commons

Sometimes Charlemagne’s military campaigns overlapped each other.  In the Saxon Wars, spanning thirty years and eighteen battles, Charlemagne eventually conquered and subdued Saxonia.  The conquering part wasn’t terribly difficult, most of the time, but the subdueing part proved nearly impossible.  Charlemagne proceeded to convert the conquered to Christianity, beginning in 773 with his campaign against the Engrians where he cut down the Saxon pagan pillar of Irminsul.  However, trouble in Italy caused that campaign to be cut short.

In 773, Charlemagne and his uncle crossed the Alps, chasing the Lombards back to Pavia which he then besieged.

Charlemagne met the Lombards at the pass in Susa Valley, shown below.

Charlemagne Susa Valley

“Susatal” by Fotogian from it. Licensed under CC BY-SA 3.0 via Commons

By 774, the siege of Pavia was over and Charlemagne had himself declared King of the Franks and Lombards and crowned with the traditional “Iron Crown of the Lombards.”

"Iron Crown" by James Steakley - photographed in the Theodelinda Chapel of the cathedral of Monza. Licensed under CC BY-SA 3.0 via Commons

“Iron Crown” by James Steakley – photographed in the Theodelinda Chapel of the cathedral of Monza. Licensed under CC BY-SA 3.0 via Commons

This crown is called “The Iron Crown” because of the narrow band of iron within the crown said to have been beaten out of the nail used at the Crucifixion of Jesus Christ.

In 776, Charlemagne was back in Saxony, having subdued the Saxons and causing their leader, Widukind, to seek refuge in Denmark.  Many Saxons were baptized as Christians.

Although the Lombards surrendered, all was not quiet on that front.  In 776, Charlemagne rushed back from Saxony to squelch a rebellion in Lombardy.

In 778, Charlemagne turned back southwards and tried to overpower the Muslim Saracen rulers of Barcelona and nearby areas.  He marched to face them, meeting them at Saragossa.  He received homage from them, but their cities did not fall.

Charlemagne was facing the toughest battle of his career where the Muslims had the upper hand and forced him to retreat. He decided to go home, since he could not trust the Basques, whom he had subdued by conquering Pamplona. He turned to leave Iberia, but as he was passing through the Pass of Roncesvalles (shown below) one of the most famous events of his long reign occurred.

Charlemagne Roncesvalles

The Basques fell on his rearguard and baggage train, utterly destroying it. The Battle of Roncevaux Pass, though less a battle than a mere skirmish, left many famous dead, including the seneschal Eggihard, the count of the palace Anselm, and the warden of the Breton March, Roland, inspiring the subsequent creation of the Song of Roland (La Chanson de Roland).

Charlemagne battle tapestry

This tapestry portraying the Battle of Roncevaux Pass was woven between 1475 and 1500.

In 779, while Charlemagne was focused elsewhere, Saxony again revolted and he again invaded and reconquered.  I’m sure by now he wondered how many times he had to do this.  He divided the land into missionary districts and personally assisted with the baptisms of masses at Lippe.

From 780-782, Saxony was quiet.  Charlemagne was back in Italy during this time.

In 782, Charlemagne returned to Saxony and was not pleased that the majority of the population was still pagan.  He implemented draconian laws prescribing death to Saxon pagans who refused to convert to Christianity.  This spurred the return of their leader, Widukind, and was followed by three years of bloody battles precipitated by the Massacre of Verden wherein Charlemagne executed 4500 trapped Saxon soldiers.  Three long years later, Widukind, defeated, accepted baptism.  At this time, the Frisians were also brought to heel.

In 787, Charlemagne focused on bringing southern Italy into the fold.  He besieged Salerno where Arechis who reigned independently submitted to vassalage.  However, upon his death in 792, Arechis’ son proclaimed independence and Charlemagne never personally returned, and was never able to bring this region fully under his control.

In 788, Charlemagne was back in Gascony trying to once again reign in a rebellion.  He had appointed his son “King” in that region and replaced Gascon individuals in power with his Frankish officers.

In 788, the pagan Asian Avars (Einhard called them Huns) had settled in Hungary and invaded Fruili and Bavaria.  Charlemagne was busy elsewhere until 790, but at the time he marched down the Danube and ravaged Avar territory.  A Lombard army did the same to Pannonia.

In 789, Charlemagne seeing an opportunity, marched into the Slavic Obotrite territory north of the Elbe, encountered little resistance, and subdued the Obotrites, sending in missionaries to convert them.  They became loyal allies, fighting alongside Charlemagne in 795 when the Saxons broke the peace.

In 792, the Saxons rebelled again in Westphalia, breaking several years of peace and distracted Charlemagne from the Avars, occupying him instead with those relentless Saxons.

However, Charlemagne’s troops continued to assault the Avars’ ring-shaped strongholds.  The great Ring of the Avars, their capital fortress, was taken twice. The booty was sent to Charlemagne at his capital, Aachen, and redistributed to all his followers and even to foreign rulers, including King Offa of Mercia. Soon the Avars had lost the will to fight and traveled to Aachen to subject themselves to Charlemagne as vassals and Christians. Charlemagne accepted their surrender and sent one native chief, baptized as Abraham, back to Avaria with the ancient title of khagan, meaning someone who rules an empire. Abraham kept his people in line, but in 800, the Bulgarians under Khan Krum also attacked the remains of Avar state.

Charlemagne Ring of Avars

From time to time, rebellions would flare up in the conquered Saxon territory.  In 793 a rebellion erupted in Eastplania and Bordalbingia, but that uprising was over by 794.  In 796, an Engrian rebellion followed.

In 794, Charlemagne set his eye upon Bavaria and was shortly thereafter dividing the land into Frankish counties, as he had done with Saxony.

From 791 to 806, Charlemagne was focused on taking the County of Toulouse for a power base and asserting his authority over the Pyrenees, making those counties vassals.

In 797, Barcelona, the greatest city of the region, previously held by the Moors, fell to Charlemagne, but was retaken in 799.  However, Louis of Aquitaine marched the entire army of his kingdom over the Pyrenees and besieged it for two years, wintering there from 800 to 801, when it capitulated.  Today, the Moorish influence can be seen in Barcelona in the architecture of the buildings.

Barcelona building

The Medieval influence can be felt in any portion of the old city and in the plazas.

barcelona plaza

I absolutely loved Barcelona when I visited in 2011, having no idea that I had ancestral history here.

Barcelona 2011

Charlemagne viewed his battle with the Muslim Moors as the battle of and for Christianity.  He wanted to convert the Muslims to Christianity.

In 799, Charlemagne conquered the Balaeric Islands, often attacked by Saracen (Moorish and Muladi) pirates.

In either 797 or 801, Charlemagne send a delegation to Baghdad where the caliph of Baghdad presented Charlemagne with an Asian elephant and a clock.  The elephant, Abul-Abbas, was transported to Charlemagne’s headquarters in Aachen where his life was chronicled.  He died in 810, possibly a war elephant, although others report that the elephant was more than 40 years old, had rheumatism, developed pneumonia while on campaign with Charlemagne, and died suddenly.  I don’t think any of my other ancestors had a pet elephant.

In 799, Pope Leo III had been mistreated by the Romans, who tried to put out his eyes and tear out his tongue.  Pope Leo escaped and fled to Charlemagne at Paderborn, asking Charlemagne to intervene in Rome and restore him. Charlemagne, advised by scholar Alcuin of York, agreed to travel to Rome, doing so in November 800 and holding a council on December 1.  On December 23, Leo swore an oath of innocence relative to the charges brought against him, and his accusers were exiled.  Two days later, at Mass, on Christmas Day, December 25th, when Charlemagne knelt at the altar to pray, the Pope crowned him Imperator Romanorum, “Emperor of the Romans,” in Saint Peter’s Basilica.  It is unclear whether Charlemagne knew this was going to happen or if the coronation was unexpected, a point debated by historians for hundreds of years.

Charlemagne coronation

Miniature of Charlemagne crowned emperor by Pope Leo III, from Chroniques de France ou de Saint Denis, vol. 1; France, second quarter of 14th century.

In 803, Charlemagne sent a Bavarian army into Pannonia ending the Avar confederation.

In 804, one last rebellion occurred in Saxony, but by this time, 30 years after being conquered, most of the original inhabitants were dead and the rest had never known anything but Charlemagne’s rule and warfare.  They were tired of fighting in their homeland.

Einhard tells us:

The war that had lasted so many years was at length ended by their acceding to the terms offered by the King; which were renunciation of their national religious customs and the worship of devils, acceptance of the sacraments of the Christian faith and religion, and union with the Franks to form one people.

In 808, an attack arrived from an unexpected source, the pagan Danes, “a race almost unknown to his ancestors, but destined to be only too well known to his sons,” as Charles Oman described them.  Wikukind, whose wife was Danish, and his allies had taken refuge among the Danes.  The king of the Danes, Godfred, built the vast Danevirke (shown below) across the isthmus of Schleswig. This defense was at its beginning a 30 km (19 mi) long defensive earthenwork rampart. The Danevirke protected Danish land and gave Godfred the opportunity to harass Frisia and Flanders with pirate raids.

Charlemagne danevirke

Godfred invaded Frisia, joked of visiting Aachen, but was murdered before he could do any more, either by a Frankish assassin or by one of his own men. Godfred was succeeded by his nephew Hemming, who concluded the Treaty of Heiligen with Charlemagne in late 811.

Europe at the end of Charlemagne’s rein looked a lot different than it did at the beginning.  The balance of power had shifted dramatically.

"Europe in 814, Charlemagne, Krum, Nicephorus I" by Stolichanin - Europe_plain_rivers.pngThe map is made according to:"World Atlas", part 3: Europe in Middle Ages, Larrouse, Paris, 2002, O. RenieAtlas "History of Bulgaria", Sofia, 1988, Bulgarian Academy of Sciences, V. Kamburova"World Atlas", N. Ostrovski, Rome, 1992, p.55Атлас "История на средните векове", Sofia, 1982, G. Gavrilov"History in maps", Johannes Herder, Berlin, 1999, p. 20"European Historical Globus", R. Rusev, 2006, p.117. Licensed under CC BY-SA 3.0 via Commons

“Europe in 814, Charlemagne, Krum, Nicephorus I” by Stolichanin – Europe_plain_rivers.pngThe map is made according to:”World Atlas”, part 3: Europe in Middle Ages, Larrouse, Paris, 2002, O. RenieAtlas “History of Bulgaria”, Sofia, 1988, Bulgarian Academy of Sciences, V. Kamburova”World Atlas”, N. Ostrovski, Rome, 1992, p.55Атлас “История на средните векове”, Sofia, 1982, G. Gavrilov”History in maps”, Johannes Herder, Berlin, 1999, p. 20″European Historical Globus”, R. Rusev, 2006, p.117. Licensed under CC BY-SA 3.0 via Commons

Education

Charlemagne was determined to have his children educated, including his daughters, as he himself was not. Below, we see Charlemagne’s monogram from the subscription of a royal diploma.  Signum Karolvs Karoli gloriosissimi regis.

Charlemagne signum

For an uneducated man, he made amazing changes, including the standardization of the monetary system and instituting principles of accounting practice.

Charlemagne’s children were taught all the arts, and his daughters were learned in the “ways of being a woman.” whatever that meant at the time. His sons participated in archery, horsemanship, and other outdoor activities.  This renaissance of education was referred to as the Carolingian Renaissance because it ushered in a new cultural era in which scholarship, literature and art thrived.

Charlemagne was brought into contact with the culture and learning of other countries (especially Moorish Spain, Anglo-Saxon England, and Lombard Italy) as a result of his vast conquests.  He greatly increased the provision of monastic schools and scriptoria (centres for book-copying) in Francia.

Most of the presently surviving works of classical Latin were copied and preserved by Carolingian scholars. Indeed, the earliest manuscripts available for many ancient texts are Carolingian. It is almost certain that a text which survived to the Carolingian age survives still, thanks to Charlemagne.

Charlemagne took a serious interest in scholarship, promoting the liberal arts at the court, ordering that his children and grandchildren be well-educated.  In a time when even leaders who promoted education did not take time to learn, Charlemagne studied.  Under the tutelage of Peter of Pisa, Charlemagne learned grammar; with Alcuin, he studied rhetoric, dialectic (logic), and astronomy (he was particularly interested in the movements of the stars); and Einhard assisted him in his studies of arithmetic.

Charlemagne’s great scholarly failure, as Einhard relates, was his inability to write: when in his old age he began attempts to learn – practicing the formation of letters in his bed during his free time on books and wax tablets he hid under his pillow – “his effort came too late in life and achieved little success.”  Charlemagne’s ability to read – which Einhard is silent about, and which no contemporary source supports – has also been called into question.

It appears that the man who was personally responsible for the salvation of so much literature was, himself, illiterate.  Charlemagne was however very foresighted and progressive.  What an amazing legacy.

Children and Heirs

Charlemagne had eighteen children over the course of his life with eight of his ten known wives or concubines.  Nonetheless, he only had four legitimate grandsons, the four sons of his fourth son, Louis. In addition, he had a grandson (Bernard of Italy, the only son of his third son, Pippin of Italy), who was born illegitimate, but included in the line of inheritance. So, despite eighteen children, the claimants to his inheritance were few.

Charlemagne’s personal life is somewhat colorful for a devout Catholic, although maybe the cultural aspect of the church at that time was different.  Then again, being the protector of the Pope may have gained Charlemagne special favors or caused some behaviors to be overlooked.Charlemagne children

As I look at the dates, I have to wonder if these women and children all lived in one place and knew each other.  Did the wife and concubine glare at each other when they met in the hallway, or were they more like compatriot sisters?  Did they live in different locations?  Did they consider themselves sucky to be Charlemagne’s partner, or did they wish things were different and that he was monogamous, as they had surely be raised in the Catholic church to expect of a husband.

I found the many references to Charlemagne’s concubines confusing, given his Catholicism and marriages at the same time, so I turned to the Catholic encyclopedia for clarification:

The Council of Toledo, held in 400, in its seventeenth canon legislates as follows for laymen (for ecclesiastical regulations on this head with regard to clerics see Celibacy): after pronouncing sentence of excommunication against any who in addition to a wife keep a concubine, it says: “But if a man has no wife, but a concubine instead of a wife, let him not be refused communion; only let him be content to be united with one woman, whether wife or concubine” (Can. “Is qui”, dist. xxxiv; Mansi, III, col. 1001). The refractory are to be excommunicated until such time as they shall obey and do penance.

It would appear, based on that edict, that Charlemagne’s concubines and extra-curricular activities were overlooked by the church, although many of his children were very active within the church as abbots and abbesses.  Charlemagne recognized and provided in some way for all of his children, legitimate or otherwise.

Charlemagne’s sons fought many wars on behalf of their father when they came of age.

Charles was mostly preoccupied with the Bretons, whose border he shared and who insurrected on at least two occasions and were easily put down, but he was also sent against the Saxons on multiple occasions. In 805 and 806, he was sent into the Böhmerwald (modern Bohemia) to deal with the Slavs living there (Bohemian tribes, ancestors of the modern Czechs). He subjected them to Frankish authority and devastated the valley of the Elbe, forcing a tribute on them.

Pippin had to hold the Avar and Beneventan borders but also fought the Slavs to his north. He was uniquely poised to fight the Byzantine Empire when finally that conflict arose after Charlemagne’s imperial coronation and a Venetian rebellion.

Finally, Louis was in charge of the Spanish March and also went to southern Italy to fight the duke of Benevento on at least one occasion. He took Barcelona in a great siege in 797.

Charlemagne’s attitude toward his daughters has been the subject of much discussion. He kept them at home with him and refused to allow them to contract sacramental marriages – possibly to prevent the creation of cadet branches of the family to challenge the main line, as had been the case with Tassilo of Bavaria – yet he tolerated their extramarital relationships, even rewarding their common-law husbands, and treasured the illegitimate grandchildren they produced for him.  At least one of them, Bertha, had a recognized relationship, if not a marriage, with Angilbert, a member of Charlemagne’s court circle.

Charlemagne also refused to believe stories of their wild behavior. He’s certainly not the first father to do that!

After Charlemagne’s death the surviving daughters were banished from the court by their brother, Louis, to take up residence in the convents they had been bequeathed by their father.  That’s what happens when you are a bit rowdy and your brother who is known as Louis the Pious becomes king.

Charlemagne’s Death

In 806, Charlemagne first made provision for the traditional division of the empire on his death. For Charles the Younger he designated Austrasia and Neustria, Saxony, Burgundy, and Thuringia. To Pippin he gave Italy, Bavaria, and Swabia. Louis received Aquitaine, the Spanish March, and Provence. There was no mention of the imperial title however, which has led to the suggestion that, at that particular time, Charlemagne regarded the title as an honorary achievement which held no hereditary significance.

This division might have worked, but it was never to be tested. Pippin died in 810 and Charles in 811. Charlemagne then reconsidered the matter, and in 813, called Louis the Pious, king of Aquitaine, his only surviving legitimate son, to his court. There Charlemagne crowned his son with his own hands as co-emperor, granting him a half-share of the empire with the rest to follow up on Charlemagne’s death.  Louis the Pious then returned to Aquitaine.  The only part of the Empire which Louis was not promised was Italy, which Charlemagne specifically bestowed upon Pippin’s illegitimate son Bernard.

Charlemagne then spent the autumn hunting before returning to Aachen on November 1st.  In January, he fell ill with pleurisy.  In a deep depression (mostly because many of his plans were not yet realized), he took to his bed on January 21st.  With all Charlemagne did achieve, I can’t imagine what he yet wanted that was severe enough to induce a depression of that magnitude.  Historians attribute his depression to his plans not being realized, but I have to wonder if the deaths of his three sons and two daughters in two years (810-811) didn’t contribute to his depression.  Another daughter had died in 808.  That’s a lot of death in a short time.

Einhard tells us:

He died January twenty-eighth, the seventh day from the time that he took to his bed, at nine o’clock in the morning, after partaking of the Holy Communion, in the seventy-second year of his age and the forty-seventh of his reign.

Charlemagne was buried the same day as his death, in Aachen Cathedral, although the cold weather and the nature of his illness made such a hurried burial unnecessary.  This causes me to wonder why he was buried so quickly.

Charlemagne Aachen Cathedral

Above, the Aachen Cathedral today.

Charlemagne is buried in the Palatine Chapel.  He commissioned its construction in 792 and it was consecrated in 805 by Pope Leo III in honor of the Virgin Mary.

"Aachener Dom Pfalzkapelle vom Münsterplatz 2014" by CaS2000 - Own work. Licensed under CC BY-SA 3.0 via Commons

“Aachener Dom Pfalzkapelle vom Münsterplatz 2014” by CaS2000 – Own work. Licensed under CC BY-SA 3.0 via Commons

Charlemagne’s floor plan, below, includes a sixteen-sided ambulatory with a gallery overhead encircling the central octagonal dome.

Charlemagne Aachen floor plan

The plan and decoration owe much to the sixth-century Basilica of San Vitale in Ravenna. Indeed, Charlemagne visited Ravenna three times, the first in 787. In that year he wrote to Pope Hadrian I and requested “mosaic, marbles, and other materials from floors and walls” in Rome and Ravenna, for his palace.

"Aix dom int vue cote" by Velvet - Own work. Licensed under CC BY-SA 3.0 via Commons

“Aix dom int vue cote” by Velvet – Own work. Licensed under CC BY-SA 3.0 via Commons

Interior view of the chapel.  Charlemagne was buried someplace here, although the exact location evades detection.  Theories abound and I have to believe that Charlemagne enjoys every minute of this mystery that his bones visit upon his descendants – which remember, includes all or most of Europe today.

"Königsthron Aachener Dom" by ​German Wikipedia user Holger Weinandt. Licensed under CC BY-SA 3.0 via Commons

“Königsthron Aachener Dom” by ​German Wikipedia user Holger Weinandt. Licensed under CC BY-SA 3.0 via Commons

Charlemagne’s throne, above, resides in the chapel as well.

Charlemagne shroud Aachen

This piece of fabric is part of Charlemagne’s death shroud, manufactured in Constantinople (present day Istanbul), and represents a quadriga, a cart or chariot that was drawn by 4 horses abreast.  Typically Gods were depicted in this manner.

A later story, told by Otho of Lomello, Count of the Palace at Aachen in the time of Otto III, about the year 1000, would claim that he and Emperor Otto had discovered Charlemagne’s tomb.  They claimed that Charlemagne was seated upon a throne, wearing a crown and holding a sceptre, his flesh almost entirely incorrupt.

In 1165, Frederick I re-opened the tomb again and placed the emperor in a sarcophagus beneath the floor of the cathedral.

Charlemagne sarcophagus

Sarcophagus, above and below.

"Karl Martell" by J. Patrick Fischer - Own work. Licensed under CC BY 2.5 via Commons

“Karl Martell” by J. Patrick Fischer – Own work. Licensed under CC BY 2.5 via Commons

In 1215 Frederick II re-interred him in a casket made of gold and silver.

Charlemagne casket

Charlemagne’s death greatly affected many of his subjects, particularly those of the literary clique who had surrounded him at Aachen.  An anonymous monk of Bobbio lamented:

From the lands where the sun rises to western shores, people are crying and wailing … the Franks, the Romans, all Christians, are stung with mourning and great worry … the young and old, glorious nobles, all lament the loss of their Caesar … the world laments the death of Charles … O Christ, you who govern the heavenly host, grant a peaceful place to Charles in your kingdom. Alas for miserable me.

Charlemagne was succeeded by his surviving son, Louis, who had been crowned the previous year.  Charlemagne’s empire lasted only another generation in its entirety; its division, according to custom, between Louis’s own sons after their father’s death laid the foundation for the modern states of Germany and France.

Below, the mask reliquary of Charlemagne.  If this is a death mask of the man, he was in wonderful shape for 72 years of age and the battles he had been through.

"Aachen Domschatz Bueste1" by Beckstet - Own work. Licensed under CC BY-SA 3.0 via Commons

“Aachen Domschatz Bueste1” by Beckstet – Own work. Licensed under CC BY-SA 3.0 via Commons

Reliquary’s were and remain very popular in the Catholic Church.  Below, Charlemagne’s arm reliquary.

"Charlemagne arm at Cathedral Treasury Aachen Germany" by Prof-Declercq - Own work. Licensed under CC BY-SA 4.0 via Commons

“Charlemagne arm at Cathedral Treasury Aachen Germany” by Prof-Declercq – Own work. Licensed under CC BY-SA 4.0 via Commons

Charlemagne’s throne remains today in the Achen Cathedral as well.

Charlemagne throne

“AachenerDomKarlsthron 1661a” by Bojin. Licensed under CC BY-SA 3.0 via Commons

Below, the interior of Charlemagne’s chapel in the Aachen Cathedral.

"Aachen-cathedral-inside". Licensed under CC BY-SA 3.0 via Commons

“Aachen-cathedral-inside”. Licensed under CC BY-SA 3.0 via Commons

What Was Charlemagne Like?

We’re exceedingly lucky that Einhard was Charlemagne’s biographer.  Otherwise we would know very little about him.

Charlemagne spoke French and German in addition to Latin and understood some Greek but spoke it very poorly.  He mandated that sermons be preached in either “Romance” (French) or “Theotiscan” (German) and not in Latin so that the common person could understand the lessons being imparted.

How Did He Look?

Charlemagne’s personal appearance is known from a description by Einhard in his biography of Charlemagne titled “Vita Karoli Magni.” Einhard describes in his twenty-second chapter:

He was heavily built, sturdy, and of considerable stature, although not exceptionally so, since his height was seven times the length of his own foot. He had a round head, large and lively eyes, a slightly larger nose than usual, white but still attractive hair, a bright and cheerful expression, a short and fat neck, and he enjoyed good health, except for the fevers that affected him in the last few years of his life. Toward the end, he dragged one leg. Even then, he stubbornly did what he wanted and refused to listen to doctors, indeed he detested them, because they wanted to persuade him to stop eating roast meat, as was his wont, and to be content with boiled meat.

The physical portrait provided by Einhard is confirmed by contemporary depictions of the emperor, such as coins and his 8-inch (20 cm) bronze statue kept in the Louvre.

"Charlemagne denier Mayence 812 814" by PHGCOM - Own work by uploader, photographed at Cabinet des Médailles, Paris.. Licensed under CC BY-SA 3.0 via Commons

“Charlemagne denier Mayence 812 814” by PHGCOM – Own work by uploader, photographed at Cabinet des Médailles, Paris.. Licensed under CC BY-SA 3.0 via Commons

It’s uncertain whether this bronze statue from the Louvre depicts Charlemagne or his grandson, Charles the Bald, who reportedly favored his grandfather, Charlemagne.

Charlemagne at Louvre

Photographs credited © RMN, Musée du Louvre / [etc.] are the property of the RMN. Non-commercial re-use is authorized, provided the source and author are acknowledged. Photo by Jean-Giles Berizzi

In 1861, Charlemagne’s tomb was opened by scientists who reconstructed his skeleton and estimated it to be measured 1.95 metres (6 ft 5 in).  A later estimate of his height from a X-ray and CT scan of his tibia performed in 2010 is 1.84 metres (6 ft 0 in). This puts him in the 99th percentile of tall people of his period, given that average male height of his time was 1.69 metres (5 ft 7 in). The width of the bone suggested he was gracile but not robust in body build.

Charlemagne wore the traditional costume of the Frankish people, described by Einhard thus:

He used to wear the national, that is to say, the Frank, dress—next his skin a linen shirt and linen breeches, and above these a tunic fringed with silk; while hose fastened by bands covered his lower limbs, and shoes his feet, and he protected his shoulders and chest in winter by a close-fitting coat of otter or marten skins.

He wore a blue cloak and always carried a sword with him. The typical sword was of a golden or silver hilt. He wore fancy jeweled swords to banquets or ambassadorial receptions.  To Charlemagne, a sword was his ever-present and indispensable weapon, just in case, and sometimes a fine piece of jewelry too.

Charlemagne robes

Nevertheless:

He despised foreign costumes, however handsome, and never allowed himself to be robed in them, except twice in Rome, when he donned the Roman tunic, chlamys, and shoes; the first time at the request of Pope Hadrian, the second to gratify Leo, Hadrian’s successor.

He could rise to the occasion when necessary. On great feast days, he wore embroidery and jewels on his clothing and shoes. He had a golden buckle for his cloak on such occasions and would appear with his great diadem, but he despised such apparel, according to Einhard, and usually dressed like the common people.

The Beautification of the Blessed Charles Augustus

Charlemagne was accorded sainthood inside the Holy Roman Empire after the twelfth century. His canonization by Antipope Paschal III, to gain the favor of Frederick Barbarossa in 1165, was never recognized by the Holy See, which annulled all of Paschal’s ordinances at the Third Lateran Council in 1179. Charlemagne’s name does not appear among the 28 saints named Charles who are listed in the Roman Martyrology. However, his beatification has been acknowledged as cultus confirmed and is celebrated on 28 January.  Even in death, Charlemagne was contentious and deeply involved in the politics of religion.

Ummmm…so how does one act appropriately if one is twice descended from a King who was also a Saint???  Do I need to learn how to curtsey or do that Queen wave maybe?  No one prepared me for this when I was learning proper manners.  I had no idea how proper “proper” was!

Afterlife

Charlemagne, being a model knight as one of the Nine Worthies, enjoyed an important afterlife in European culture.

The Nine Worthies are nine historical, scriptural and legendary personages who personify the ideals of chivalry as were established in the Middle Ages.

The Nine Worthies include three good pagans: Hector, Alexander the Great, and Julius Caesar, three good Jews: Joshua, David, and Judas Maccabeus, and three good Christians: King Arthur, Charlemagne, and Godfrey of Bouillon.

Gateway Ancestors

If you’d like to see if you too are descended from Charlemagne through known gateway ancestors, please click here for a list.  But beware, you just might have to learn how to behave “properly.”  If you figure out exactly what that means, let me know.

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Irene Charitas (c1665-c1694) and Her Aching Mother’s Soul, 52 Ancestors #100

For a very long time, and on most online trees, Irene Charitas is listed as the wife of Johann Michael Mueller who was born about 1655 in Zollikoffen, Switzerland and who died in 1695 in Steinwenden, Germany.  Her last name is listed as Charitas, but it isn’t.  Charitas is Irene’s middle name.

At this time in history, in Germany and the Germanic speaking Protestant regions of Europe, females were given two names, a first “saints” name and a second name by which they were typically called.  Irene is quite unusual for a Saint’s name and Charitas is very unusual for a middle name.  So unusual in fact that I’ve only seen it one other time, ever.

Charitas is a Latin word meaning charity and “for the love of God.”  Charitas, or charity, is one of the 7 virtues.

Steinwenden 5

We don’t know where Irene was born, but what we do know is that we first find her as Johann Michael Mueller’s wife in Steinwenden, Germany, shown above and below, when she gives birth to the first child recorded to this couple in the church records in 1685.  Could this couple have lived there, or had children elsewhere, previously?  Of course.  Could they have had other children that were baptized in a different church?  Yes.  Michael was born in 1655, so if Irene was his age, they could have married by about 1673-1675 and had another 5 children or so before they appear in the Steinwenden records.  But did they?  We’ll never know for sure, but there is no evidence today to suggest such.

Steinwenden 6

Unfortunately, the only part of the original Steinwenden church that survives today is the bell tower.

Steinwenden 4

Sometime in the early 1680s, the Mueller family arrived in Steinwenden from the Zollikoffen area near Bern, Switzerland. It’s likely that Irene’s family was among the same immigrant group – but we just don’t know and to the best of my knowledge, no research has been done on that topic.  Furthermore, the Johann Michael Mueller family could have made an intermittent stop along the way that we are unaware of.  In other words, Michael could have married Irene Charitas anyplace between Zollikoffen and Steinwenden.

I’m not quite sure how Charitas became her last name on the internet.  Perhaps it’s an assumption based on the fact that her middle name is an unfamiliar name and someone assumed it was her last name.  In any event, it’s been that way for years now and I’m hopeful that records from the actual church can help reduce or eliminate this misinformation.  I’m currently in the process of having the church records retranslated by a professional German genealogist, just to be sure.

When our cousin, the Reverend Richard Miller visited the church in Steinwenden in 1996, the church historians and a German genealogist prepared a summary of the church records involving Johann Michael Mueller, shown below.

In all of the birth records of children born to Michael, Irene Charitas was his wife, and Charitas was not her birth name.  If the child born in 1685 was their first, then Irene Charitas was likely born about 1665, give or take a couple years in either direction.

Steinwenden 7

Recently, Richard sent me the original record of Johann Michael Mueller’s birth from the Steinwenden church.  It’s the second to last entry, below.

Miller 1792 birth steinwenden

Needless to say, I can’t read this, on two fronts, the language and the script, which is why I’m having this and the other records retranslated.

The next we hear of Irene is a church record for a confirmation of Irene Charitas Schlosser, a daughter of Conrad Schlosser, of Steinwinden.  Often, children were named after their godparents with the idea that the Godparents were relatives and they were the appointed relatives responsible for the religious education of the child – and whether spoken or unspoken, it was also expected that if the parents died, the Godparents would raise the children – or at least the one(s) named for them.  Unfortunately, in the age of marginal medical care, no antibiotics and an era where every pregnancy was high risk, that happened all too often.  So, it appears that Conrad Schlosser’s daughter was named for Michael Mueller’s wife, Irene Charitas.

It’s likely that Irene was in some way related to Conrad.  She could have been his sister or aunt or a favorite cousin.  Or, Conrad could have been related to Johann Michael Mueller.  One way or another Conrad trusted Irene enough to name his daughter after her, making Irene Charitas Mueller the first in line to raise her namesake should something happen to Conrad and his wife.  Additional research on the Schlosser family church records is in order.

Steinwenden 1

The first record on this transcriptions says that Jacob Ringeisen of Schweitz was “serving for his cousin” Michael Muller.

In other words, even though the daughter was named for Irene, Michael’s wife, Irene wasn’t present, possibly due to pregnancy herself, and apparently neither was Michael.  However, Michael’s cousin in essence represented Michael and the couple’s commitment at the baptism.

Of course, now this makes me ask just how Michael and Jacob were cousins, and was it through marriage via Irene Charitas?  It looks like we may have yet another family connection hint.  So often in these old church records there is so much more buried in the details that is missed if all you get is a translation of the actual “event.”

In genealogy, always, always, more questions.

Irene Charitas’ life was short.  She probably died before she was 30.  There are no more known records of her, at least not directly.

What we do know is that the last child in these church records is born to Irene and Michael in 1692.  This is the only one of the six children she bore that lived.  This is an incredibly sad story that seems to stretch beyond just “bad luck.”   

Child Birth Death Age at Death
Johann Nicholas Muller June 5, 1685 June 6, 1685 1 day
Johann Abraham Muller July 9, 1688 1696 Less than 6 months
Samuel Muller April 30, 1687 April 30, 1687 Shortly after birth
Catherine Barbara Muller June 7, 1688 June 21, 1691 3 years, 2 weeks
Eva Catherine Muller April 24, 1691 June 29, 1691 2 months
Johann Michael Muller October 5, 1692 1771 78 years

Looking at these children’s deaths, I find the month of June, 1691 particularly heartbreaking.  Clearly, something contagious was occurring and both of Irene’s children died, 8 days apart.  I wonder if the church records reflect a rash of deaths within the village.  Just 11 months later, she would bear her 6th child.  I bet those months between June of 1691 and May of 1692 were living Hell for Irene, between the sorrow and grief of losing her children and the uncertainly of the one she was carrying.

Fortunately for me, Johann Michael Mueller, the second, born in 1692, named after his father, did live, as he is my ancestor.

Johann Michael Mueller Sr. died in Steinwenden in 1695, just three years later. For some reason, from 1692 to 1695, there were no more children born to Johann Michael Mueller and Irene Charitas – nor to Johann Michael Mueller and anyone else.

Why is this important?  Because another rumor that has been rampant over the years is that Johann Michael Mueller was married to Anna Loysa Regina and that she was the mother of Johann Michael Mueller.  At least I was able to figure out where this information originated.

On September 29, 1695, Anna Loysa Regina married Jacob Stutzman in Steinwenden, although I have not seen the original record myself.  She is noted at that time as being the widow of Michael Mueller.

For Anna Loysa Regina to be the window of Michael Mueller, that means that Irene Charitas died sometime after giving birth to Michael Jr. on October 5, 1692 and sometime before Michael Sr.’s death on January 31, 1695, just 2 years and 3 months later, allowing Michael Sr. enough time to remarry after Irene’s death and before his own.  Remarriage often didn’t take much time actually, given that most people already knew each other through church and it was simply a matter of taking stock of the available spouses and making a choice from the willing and most compatible selection.  No, it was not about love but one would hope it was at least about like and that love evolved.  Regardless, marriage was a practical matter of survival as men and women needed each other’s assistance in the daily activities of living and raising children.  Michael would have had a small child who needed a mother.

Michael and Anna Loysa Regina must not have been married long, because there are no children recorded to she and Michael, but she did go on to have children with Jacob Stutzman, one as late as 1706.  Jacob Stutzman Jr. born in 1706, and Johann Michael Mueller Jr., born in 1692, step-brothers of a sort, would in 1727 immigrate to America together.

If all of these records have been accurately translated, Irene Charitas probably died about 1694, possibly in childbirth.  In the natural order of things, March or April 1694 would be about 18 months after Michael was born, representing the typical spacing between children.  Why no record of her death exists in the church records is a mystery.  Perhaps we need to look again, and maybe in the surrounding church records as well.

Cemetery plots in Germany, as is customary in Europe, are reused.  In some cases, they continue within the family, with generation upon generation (pardon the pun) being buried in the same location.  In other cases, the grave is considered “abandoned” if no one pays upkeep, and the site is reused at the discretion of the church.  Gravesites that aren’t abandoned are still reused, but generally by the family and perhaps not as quickly as abandoned graves.  While this is very foreign to those of us in the US, if Europeans did not employ some “recycling” burial strategy, the entire continent would be blanketed with cemeteries and there would no room for the living.

Being someone who wonders about everything, I asked at a Dutch church during a European visit in 2014 about what happened if there were still bones in the grave when they set about burying the next person.  I’m glad I asked, because I then discovered that those little buildings in or near cemeteries weren’t what I thought.  I assumed they were the gardener’s or sextant’s shed, containing things like shovels, lawnmowers, etc.  Well, I was wrong.  Those little buildings are ossuaries containing the bones of the former inhabitants of graves.  The photo below is the ossuary in Wolsum, the Netherlands.

Ossuary Wolsum

This is truly the final resting place until the bones turn to dust, generally stacked something like cordwood with similar types of bones stacked with like bones on shelves.  Yes, seriously.  Once moved from the grave to the ossuary, the bones are not kept together as a “person.”  This photo is an ossuary in Hallstatt, Austria.

Ossuary Austria

From a DNA perspective, these ossuaries, found in almost all cemeteries, are just torture to me, because I can just see the DNA of my ancestral lines in that ossuary, all mixed in with the DNA of the other families…which are probably mine as well, given that these people married their neighbors in the community for generations.  There they are, my ancestors and their DNA, right in front of me, but entirely anonymous and completely unidentifiable.  If we knew who they were, we could obtain the Y and mtDNA lineage of every family in the village, including mine!

The bones in the ossuaries are just waiting to finish turning to dust – a process that takes longer than they are allowed to rest in the ground.  So a grave in Europe is not a place of perpetual rest, it’s a temporary resting point but not the last stop on the journey.  I just can’t help but think what a wonderful scientific study it would be to analyze the bones in an ossuary and compare the results to the DNA of the current village inhabitants, and those descendants who moved away.  And yes, you know I’d be in the front of the line, volunteering.  You could reconstruct an entire village in the 1700s or maybe 1800s from their DNA – maybe even further back.  You could tell who settled there, where they were from originally… you could learn so much.  But back to reality….

Not only do we not know where Irena Charitas and her infant children were buried, their dust assuredly shares that location today with several subsequent generations of Germans, most likely not her descendants because her only known descendant immigrated to America in 1727 with his Stutzman step-brother.  Irene Charitas’ son Johann Michael Mueller, Jr. never knew his mother or father, never remembered seeing his mother’s face, beaming down at him, so joyous that he was alive.  He had no memory of her loving touch.  He was raised by his step-mother and her subsequent husband, Jacob Stutzman, after both of Michael’s parents died by the time he was three.

There was no happy ending for Irene Charitas.  In fact – it seems that her entire adult lifetime was filled with serial grief, except for those few brief months when she and baby Michael both lived.  Irene Charitas’ grief was caused by the births and deaths of 5 children in 5 years, followed by her own death not long after her 6th child was born and survived.  Then, the terrible irony.  When a child finally lives, she herself succumbs.

I can only imagine the excitement Irene felt about her first pregnancy, followed by the shattering death of the baby.  Surely, she would have told herself that it wouldn’t happen again.  It was a first birth, probably difficult.  The second one would be easier.  Just a few months later, she became pregnant again and full of hope, only to have her dreams shattered again with the death of that child.  And then again…and again and again, year after year after year.  Just five years after that first baby died, she was pregnant for her sixth child.  I wonder if she started out in dread when she discovered she was pregnant again, never allowing herself to be excited, to plan, to hope for that baby to live.  I could understand how she might feel that way after 5 dead babies in 5 years.  I know how frightened I was when I was pregnant for my third child after my second child died.

And then the baby lived but she died.  Oh, the horrible irony.  Poor Irene.  In death, leaving behind her one child that lived.  She must have fought the grim reaper with every ounce of her being until the very end.  But it wasn’t enough.  It just wasn’t enough.

I hope that Irene Charitas was able to see, from afar, her son, Johann Michael Mueller Jr. growing up strong, being raised by his step-mother and step-father in a pious pietist home and that it helped sooth her aching mother’s soul.

4-15-2018 Update – We now have a surname and parents for Irene.  Click here to read the next article!

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I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

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Mass Pre-Contact Native Grave in California Yields Disappointing Results

In 2012 during excavation for a shopping mall near San Francisco, a mass grave containing 7 men was unearthed.  The manner in which they were buried led archaeologists to believe that they had been murdered, and quickly buried, not ceremonially buried as tribal members would be.  They were found among more than 200 other burials.

The victims ages ranged from about 18 to about 40 and scientists concentrated on analyzing the wounds, cause of death and DNA of these men.  In part, they wanted to see if they were related to each other and if they originated in this area or came from elsewhere.  In other words, were they unsuccessful invaders as suggested by the circumstances of their burials?

This article tells more about the excavations and includes some photos.

Analysis suggests the men lived about 1200 years ago, clearly before European contact.  Analysis of the men’s teeth provided information about their history.  These men had spent their lives together, but their isotope signatures were clearly different than the individuals in the balance of the burials.  Indeed, they look to have been invaders.

An academic paper titled “Isotopic and genetic analysis of a mass grave in central California: Implications for precontact hunter-gatherer warfare” was published a few weeks ago in the American Journal of Physical Anthropology.  The article itself is behind a paywall available here.  The abstract is provided below:

Abstract

OBJECTIVES:

Analysis of a mass burial of seven males at CA-ALA-554, a prehistoric site in the Amador Valley, CA, was undertaken to determine if the individuals were “locals” or “non-locals,” and how they were genetically related to one another.

METHODS:

The study includes osteological, genetic (mtDNA), and stable (C, N, O, S) and radiogenic (Sr) isotope analyses of bone and tooth (first and third molars) samples.

RESULTS:

Isotopes in first molars, third molars, and bone show they spent the majority of their lives living together. They are not locals to the Amador Valley, but were recently living to the east in the San Joaquin Valley, suggesting intergroup warfare as the cause of death. The men were not maternally related, but represent at least four different matrilines. The men also changed residence as a group between age 16 and adult years.

CONCLUSIONS:

Isotope data suggest intergroup warfare accounts for the mass burial. Genetic data suggest the raiding party included sets of unrelated men, perhaps from different households. Generalizing from this case and others like it, we hypothesize that competition over territory was a major factor behind ancient warfare in Central California. We present a testable model of demographic expansion, wherein villages in high-population-density areas frequently fissioned, with groups of individuals moving to lower-population-density areas to establish new villages. This model is consistent with previous models of linguistic expansion. Am J Phys Anthropol, 2015. © 2015 Wiley Periodicals, Inc.

http://www.ncbi.nlm.nih.gov/pubmed/26331533

Genetic Information

I was extremely disappointed with the genetic information.  Working with the local Ohlone community, the scientists did attempt to extract DNA from the 7 individuals in the mass grave, with 6 extractions being successful.

They only analyzed the HVR1 region of the mitochondrial DNA.

Eerkens 2015 table

In the paper, the authors indicate that nuclear DNA which would include the Y chromosome as well as autosomal DNA was too degraded to recover.  While disappointing, there is nothing they can do about that.

However, only analyzing the mitochondrial DNA, which they clearly were able to amplify, at the HVR1 level is an incredible lost opportunity.  They obtained enough resolution in 6 of the individuals to obtain general haplogroup assignments.  However, the HVR2 and coding regions would have provided the defining information about extended haplogroups and individual mutations, including, perhaps, haplogroups rarely or never seen previously in the Americas.

Furthermore, given the information above, we can’t tell if the D1 individuals are related to each other matrilineally or not.  The B2 individuals are clearly not related in a recent timeframe nor are the A2, B2 and D1 people related to each other on their matrilineal line.  What a shame more information wasn’t obtained.

While I’m grateful that DNA testing was undertaken, I’m saddened by the partial results, especially in this day of full genomic sequencing for ancient DNA specimens.  I’m perplexed as to why they would not have obtained as much information as was possible, given the significant effort expended in recovering any ancient DNA specimen.

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Some Native Americans Had Oceanic Ancestors

This week has seen a flurry of new scientific and news articles.  What has been causing such a stir?  It appears that Australian or more accurately, Australo-Melanese DNA has been found in South America’s Native American population. In addition, it has also been found in Aleutian Islanders off the coast of Alaska.  In case you aren’t aware, that’s about 8,500 miles as the crow flies.  That’s one tired crow.  As the person paddles or walks along the shoreline, it’s even further, probably about 12,000 miles.

Aleutians to Brazil

Whatever the story, it was quite a journey and it certainly wasn’t all over flat land.

This isn’t the first inkling we’ve had.  Just a couple weeks ago, it was revealed that the Botocudo remains from Brazil were Polynesian and not admixed with either Native, European or African.  This admixture was first discovered via mitochondrial DNA, but full genome sequencing confirmed their ancestry and added the twist that they were not admixed – an extremely unexpected finding.  This is admittedly a bit confusing, because it implies that there were new Polynesian arrivals in the 1600s or 1700s.

Unlikely as it seems, it obviously happened, so we set that aside as relatively contemporary.

The findings in the papers just released are anything but contemporary.

The First Article

The first article in Science, “Genomic evidence for the Pleistocene and recent population history of Native Americans” by Raghaven et al published this week provides the following summary (bolding is mine):

How and when the Americas were populated remains contentious. Using ancient and modern genome-wide data, we find that the ancestors of all present-day Native Americans, including Athabascans and Amerindians, entered the Americas as a single migration wave from Siberia no earlier than 23 thousand years ago (KYA), and after no more than 8,000-year isolation period in Beringia. Following their arrival to the Americas, ancestral Native Americans diversified into two basal genetic branches around 13 KYA, one that is now dispersed across North and South America and the other is restricted to North America. Subsequent gene flow resulted in some Native Americans sharing ancestry with present-day East Asians (including Siberians) and, more distantly, Australo-Melanesians. Putative ‘Paleoamerican’ relict populations, including the historical Mexican Pericúes and South American Fuego-Patagonians, are not directly related to modern Australo-Melanesians as suggested by the Paleoamerican Model.

This article in EurekAlert and a second one here discuss the Science paper.

Raghaven 2015

Migration map from the Raghaven paper.

The paper included the gene flow and population migration map, above, along with dates.

The scientists sequenced the DNA of 31 living individuals from the Americas, Siberia and Oceana as follows:

Siberian:

  • Altai – 2
  • Buryat – 2
  • Ket – 2
  • Kiryak – 2
  • Sakha – 2
  • Siberian Yupik – 2

North American Native:

  • Tsimshian (number not stated, but by subtraction, it’s 1)

Southern North American, Central and South American Native:

  • Pima – 1
  • Huichol -1
  • Aymara – 1
  • Yakpa – 1

Oceana:

  • Papuan – 14

The researchers also state that they utilized 17 specimens from relict groups such as the Pericues from Mexico and Fuego-Patagonians from the southernmost tip of South America.  They also sequenced two pre-Columbian mummies from the Sierra Tarahumara in northern Mexico.  In total, 23 ancient samples from the Americas were utilized.

They then compared these results with a reference panel of 3053 individuals from 169 populations which included the ancient Saqqaq Greenland individual at 400 years of age as well as the Anzick child from Montana from about 12,500 years ago and the Mal’ta child from Siberia at 24,000 years of age.

Not surprisingly, all of the contemporary samples with the exception of the Tsimshian genome showed recent western Eurasian admixture.

As expected, the results confirm that the Yupik and Koryak are the closest Eurasian population to the Americas.  They indicate that there is a “clean split” between the Native American population and the Koryak about 20,000 years ago.

They found that “Athabascans and Anzick-1, but not the Greenlandis Inuit and Saqqaq belong to the same initial migration wave that gave rise to present-day Amerindians from southern North America and Central and South America, and that this migration likely followed a coastal route, given our current understanding of the glacial geological and paleoenvironmental parameters of the Late Pleistocene.”

Evidence of gene flow between the two groups was also found, meaning between the Athabascans and the Inuit.  Additionally, they found evidence of post-split gene flow between Siberians and Native Americans which seems to have stopped about 12,000 years ago, which meshes with the time that the Beringia land bridge was flooded by rising seas, cutting off land access between the two land masses.

They state that the results support all Native migration from Siberia, contradicting claims of an early migration from Europe.

The researchers then studied the Karitiana people of South America and determined that the two groups, Athabascans and Karitiana diverged about 13,000 years ago, probably not in current day Alaska, but in lower North America.  This makes sense, because the Clovis Anzick child, found in Montana, most closely matches people in South America.

By the Clovis period of about 12,500 years ago, the Native American population had already split into two branches, the northern and southern, with the northern including Athabascan and other groups such as the Chippewa, Cree and Ojibwa.  The Southern group included people from southern North America and Central and South America.

Interestingly, while admixture with the Inuit was found with the Athabascan, Inuit admixture was not found among the Cree, Ojibwa and Chippewa.  The researchers suggest that this may be why the southern branch, such as the Karitiana are genetically closer to the northern Amerindians located further east than to northwest coast Amerindians and Athabascans.

Finally, we get to the Australian part.  The researchers when trying to sort through the “who is closer to whom” puzzle found unexpected results.  They found that some Native American populations including Aleutian Islanders, Surui (Brazil) and Athabascans are closer to Australo-Melanesians compared to other Native Americans, such as Ojibwa, Cree and Algonquian and South American Purepecha (Mexico), Arhuaco (Colombia) and Wayuu (Colombia, Venezuela).  In fact, the Surui are one of the closest populations to East Asians and Australo-Melanese, the latter including Papuans, non-Papuan Melanesians, Solomon Islanders and hunter-gatherers such as Aeta. The researchers acknowledge these are weak trends, but they are nonetheless consistently present.

Dr. David Reich, from Harvard, a co-author of another paper, also published this past week, says that 2% of the DNA of Amazonians is from Oceana.  If that is consistent, it speaks to a founder population in isolation, such that the 2% just keeps getting passed around in the isolated population, never being diluted by outside DNA.  I would suggest that is not a weak signal.

The researchers suggest that the variance in the strength of this Oceanic signal suggests that the introduction of the Australo-Melanese occurred after the initial peopling of the Americas.  The ancient samples cluster with the Native American groups and do not show the Oceanic markers and show no evidence of gene flow from Oceana.

The researchers also included cranial morphology analysis, which I am omitting since cranial morphology seems to have led researchers astray in the past, specifically in the case of Kennewick man.

One of the reasons cranial morphology is such a hotly debated topic is because of the very high degree of cranial variance found in early skeletal remains.  One of the theories evolving from the cranial differences involving the populating of the Americans has been that the Australo-Melanese were part of a separate and earlier migration that gave rise to the earliest Americans who were then later replaced by the Asian ancestors of current day Native Americans.  If this were the case, then the now-extinct Fuego-Patagonains samples from the location furthest south on the South American land mass should have included DNA from Oceana, but it didn’t.

The Second Article

A second article published this week, titled “’Ghost population’ hints at long lost migration to the Americas” by Ellen Callaway discusses similar findings, presented in a draft letter to Nature titled “Genetic evidence for two founding populations of the Americas” by Skoglund et al.  This second group discovers the same artifact Australo-Melanesian DNA in Native American populations but suggests that it may be from the original migration and settlement event or that there may have been two distinct founding populations that settled at the same time or that there were two founding events.

EurekAlert discusses the article as well.

It’s good to have confirmation and agreement between the two labs who happened across these results independently that the Australo-Melanesian DNA is present in some Native populations today.

Their interpretations and theories about how this Oceanic DNA arrived in some of the Native populations vary a bit, but if you read the details, it’s really not quite as different as it first appears from the headlines.  Neither group claims to know for sure, and both discuss possibilities.

Questions remain.  For example, if the founding group was small, why, then, don’t all of the Native people and populations have at least some Oceanic markers?  The Anzick Child from 12,500 years ago does not.  He is most closely related to the tribes in South America, where the Oceanic markers appear with the highest frequencies.

In the Harvard study, the scientists fully genome sequenced 63 individuals without discernable evidence of European or African ancestors in 21 Native American populations, restricting their study to individuals from Central and South America that have the strongest evidence of being entirely derived from a homogenous First American ancestral population.

Their results show that the two Amazonian groups, Surui and Karitians are closest to the “Australasian populations, the Onge from the Andaman Island in the Bay of Bengal (a so-called ‘Negrito’ group), New Guineans, Papuans and indigenous Australians.”  Within those groups, the Australasian populations are the only outliers – meaning no Africans, Europeans or East Asian DNA found in the Native American people.

When repeating these tests, utilizing blood instead of saliva, a third group was shown to also carry these Oceanic markers – the Xavante, a population from the Brazilian plateau that speaks a language of the Ge group that is different from the Tupi language group spoke by the Karitians and Surui.

Skoglund 2015-2

The closest populations that these Native people matched in Oceana, shown above on the map from the draft Skoglund letter, were, in order, New Guineans, Papuans and Andamanese.  The researchers further state that populations from west of the Andes or north of the Panama isthmus show no significant evidence of an affinity to the Onge from the Andaman Islands with the exception of the Cabecar (Costa Rica).

That’s a very surprising finding, given that one would expect more admixture on the west, which is the side of the continent where the migration occurred.

The researchers then compared the results with other individuals, such as Mal’ta child who is known to have contributed DNA to the Native people today, and found no correlation with Oceanic DNA.  Therefore, they surmised that the Oceanic admixture cannot be explained by a previously known admixture event.

They propose that a mystery population they have labeled as “Population Y” (after Ypykuera which means ancestor in the Tupi language family) contributed the Australasian lineage to the First Americans and that is was already mixed into the lineage by the time it arrived in Brazil.

According to their work, Population Y may itself have been admixed, and the 2% of Oceanic DNA found in the Brazilian Natives may be an artifact of between 2 and 85% of the DNA of the Surui, Karitiana and Xavante that may have come from Population Y.  They mention that this result is striking in that the majority of the craniums that are more Oceanic in Nature than Asiatic, as would be expected from people who migrated from Siberia, are found in Brazil.

They conclude that the variance in the presence or absence of DNA in Native people and remains, and the differing percentages argue for more than one migration event and that “the genetic ancestry of Native Americans from Central and South America cannot be due to a single pulse of migration south of the Late Pleistocene ice sheets from a homogenous source population, and instead must reflect at least two streams of migration or alternatively a long drawn out period of gene flow from a structured Beringian or Northeast Asian source.”

Perhaps even more interesting is the following statement:

“The arrival of population Y ancestry in the Americas must in any scenario have been ancient: while Population Y shows a distant genetic affinity to Andamanese, Australian and New Guinean populations, it is not particularly closely related to any of them, suggesting that the source of population Y in Eurasia no longer exists.”

They further state they find no admixture indication that would suggest that Population Y arrived in the last few thousand years.

So, it appears that perhaps the Neanderthals and Denisovans were not the only people who were our ancestors, but no longer exist as a separate people, only as an admixed part of us today.  We are their legacy.

The Take Away

When I did the Anzick extractions, we had hints that something of this sort might have been occurring.  For example, I found surprising instances of haplogroup M, which is neither European, African nor Native American, so far as we know today.  This may have been a foreshadowing of this Oceanic admixture.  It may also be a mitochondrial artifact.  Time will tell.  Perhaps haplogroup M will turn out to be Native by virtue of being Oceanic and admixed thousands of years ago.  There is still a great deal to learn.  Regardless of how these haplogroups and Oceanic DNA arrived in Brazil in South America and in the Aleutian Islands off of Alaska, one thing is for sure, it did.

We know that the Oceanic DNA found in the Brazilian people studied for these articles is not contemporary and is ancient.  This means that it is not related to the Oceanic DNA found in the Botocudo people, who, by the way, also sport mitochondrial haplogroups that are within the range of Native people, meaning haplogroup B, but have not been found in other Native people.  Specifically, haplogroups B4a1a1 and B4a1a1a.  Additionally, there are other B4a1a, B4a1b and B4a1b1 results found in the Anzick extract which could also be Oceanic.  You can see all of the potential and confirmed Native American mitochondrial DNA results in my article “Native American Mitochondrial Haplogroups” that I update regularly.

We don’t know how or when the Botocudo arrived, but the when has been narrowed to the 1600s or 1700s.  We don’t know how or when the Oceanic DNA in the Brazilian people arrived either, but the when was ancient.  This means that Oceanic DNA has arrived in South America at least twice and is found among the Native peoples both times.

We know that some Native groups have some Oceanic admixture, and others seem to have none, in particular the Northern split group that became the Cree, Ojibwa, Algonquian, and Chippewa.

We know that the Brazilian Native groups are most closely related to Oceanic groups, but that the first paper also found Oceanic admixture in the Aleutian Islands.  The second paper focused on the Central and South American tribes.

We know that the eastern American tribes, specifically the Algonquian tribes are closely related to the South Americans, but they don’t share the Oceanic DNA and neither do the mid-continent tribes like the Cree, Ojibwa and Chippewa.  The only Paleolithic skeleton that has been sequenced, Anzick, from 12,500 years ago in Montana also does not carry the Oceanic signature.

In my opinion, the disparity between who does and does not carry the Oceanic signature suggests that the source of the Oceanic DNA in the Native population could not have been a member of the first party to exit out of Beringia and settle in what is now the Americas.  Given that this had to be a small party, all of the individuals would have been thoroughly admixed with each other’s ancestral DNA within just a couple of generations.  It would have been impossible for one ancestor’s DNA to only be found in some people.  To me, this argues for one of two scenarios.

First, a second immigration wave that joined the first wave but did not admix with some groups that might have already split off from the original group such as the Anzick/Montana group.

Second, multiple Oceanic immigration events.  We still have to consider the possibility that there were multiple events that introduced Oceanic DNA into the Native population.  In other words, perhaps the Aleutian Islands Oceanic DNA is not from the same migration event as the Brazilian DNA which we know is not from the same event as the Botocudo.  I would very much like to see the Oceanic DNA appear in a migration path of people, not just in one place and then the other.  We need to connect the dots.

What this new information does is to rule out the possibility that there truly was only one wave of migration – one group of people who settled the Americas at one time.  More likely, at least until the land bridge submerged, is that there were multiple small groups that exited Beringia over the 8,000 or so years it was inhabitable.  Maybe one of those groups included people from Oceana.  Someplace, sometime, as unlikely as it seems, it happened.

The amazing thing is that it’s more than 10,000 miles from Australia to the Aleutian Islands, directly across the Pacific.  Early adventurers would have likely followed a coastal route to be sustainable, which would have been significantly longer.  The fact that they survived and sent their DNA on a long adventure from Australia to Alaska to South America – and it’s still present today is absolutely amazing.

Australia to Aleutians

We know we still have a lot to learn and this is the tip of a very exciting iceberg.  As more contemporary and ancient Native people have their full genomes sequenced, we’ll learn more answers.  The answer is in the DNA.  We just have to sequence enough of it and learn how to understand the message being delivered.

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I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

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Botocudo Ancient Remains from Brazil

Update: Please note that I am leaving this article because the scientific information is accurate, BUT, it was subsequently discovered that the remains were mislabeled in the museum and were not Native.

One thing you can always count on in the infant science of population genetics…  whatever you think you know, for sure, for a fact…well….you don’t.  So don’t say too much, too strongly or you’ll wind up having to decide if you’d like catsup with your crow!  Well, not literally, of course.  It’s an exciting adventure that we’re on together and it just keeps getting better and better.  And the times…they are a changin’.

We have some very interesting news to report.  Fortunately, or unfortunately – the news weaves a new, but extremely interesting, mystery.

Ancient Mitochondrial DNA

Back in 2013, a paper, Identification of Polynesian mtdNA haplogroups in remains of Botocudo Amerindians from Brazil, was published that identified both Native American and Polynesian haplogroups in a group of 14 skeletal remains of Botocudo Indians from Brazil whose remains arrived at a Museum in August of 1890 and who, the scientists felt, died in the second half of the 19th century.

Twelve of their mitochondrial haplogroups were the traditional Native haplogroup of C1.

However, two of the skulls carried Polynesian haplogroups, downstream of haplogroup B, specifically B4a1a1a and B4a1a1, that compare to contemporary individuals from Polynesian, Solomon Island and Fijian populations.  These haplotypes had not been found in Native people or previous remains.

Those haplogroups include what is known as the Polynesian motif and are found in Indonesian populations and also in Madagascar, according to the paper, but the time to the most common recent ancestor for that motif was calculated at 9,300 years plus or minus 2000 years.  This suggests that the motif arose after the Asian people who would become the Native Americans had already entered North and South America through Beringia, assuming there were no later migration waves.

The paper discusses several possible scenarios as to how a Polynesian haplotype found its way to central Brazil among a now extinct Native people. Of course, the two options are either pre-Columbian (pre-1500) contact or post-Columbian contact which would infer from the 1500s to current and suggests that the founders who carried the Polynesian motif were perhaps either slaves or sailors.

In the first half of the 1800s, the Botocudo Indians had been pacified and worked side by side with African slaves on plantations.

Beyond that, without full genome sequencing there was no more that could be determined from the remains at that time.  We know they carried a Polynesian motif, were found among Native American remains and at some point in history, intermingled with the Native people because of where they were found.  Initial contact could have been 9,000 years ago or 200.  There was no way to tell.  They did have some exact HVR1 and HVR2 matches, so they could have been “current,” but I’ve also seen HVR1 and HVR2 matches that reach back to a common ancestor thousands of years ago…so an HVR1/HVR2 match is nothing you can take to the bank, certainly not in this case.

Full Genome Sequencing and Y DNA

This week, one on my subscribers, Kalani, mentioned that Felix Immanuel had uploaded another two kits to GedMatch of ancient remains.  Those two kits are indeed two of the Botocudo remains – the two with the Polynesian mitochondrial motif which have now been fully sequenced.  A corresponding paper has been published as well, “Two ancient genomes reveal Polynesian ancestry among the indigenous Botocudos of Brazil” by Malaspinas et al with supplemental information here.

There are two revelations which are absolutely fascinating in this paper and citizen scientist’s subsequent work.

First, their Y haplogroups are C-P3092 and C-Z31878, both equivalent to C-B477 which identifies former haplogroup C1b2.  The Y haplogroups aren’t identified in the paper, but Felix identified them in the raw data files that are available (for those of you who are gluttons for punishment) at the google drive links in Felix’s article Two Ancient DNA from indigenous Botocudos of Brazil.

I’ve never seen haplogroup C1b2 as Native American, but I wanted to be sure I hadn’t missed a bus, so I contacted Ray Banks who is one of the administrators for the main haplogroup C project at Family Tree DNA and also is the coordinator for the haplogroup C portion of the ISOGG tree.

ISOGG y tree

You can see the position of C1b2, C-B477 in yellow on the ISOGG (2015) tree relative to the position of C-P39 in blue, the Native American SNP shown several branches below, both as branches of haplogroup C.

Ray maintains a much more descriptive tree of haplogroup C1 at this link and of C2 at this link.

Ray Banks C1 tree

The branch above is the Polynesian (B477) branch and below, the Native American (P39) branch of haplogroup C.

Ray Banks C2 treeIn addition to confirming the haplogroup that Felix identified, when Ray downloaded the BAM files and analyzed the contents, he found that both samples were also positive for M38 and M208, which moves them downstream two branches from C1b2 (B477).

Furthermore, one of the samples had a mutation at Z32295 which Ray has included as a new branch of the C tree, shown below.

Ray Banks Z32295

Ray indicated that the second sample had a “no read” at Z32295, so we don’t know if he carried this mutation.  Ray mentions that both men are negative for many of the B459 equivalents, which would move them down one more branch.  He also mentioned that about half of the Y DNA sites are missing, meaning they had no calls in the sequence read.  This is common in ancient DNA results.  It would be very interesting to have a Big Y or equivalent test on contemporary individuals with this haplogroup from the Pacific Island region.

Ray notes that all Pacific Islanders may be downstream of Z33295.

Not Admixed

The second interesting aspect of the genomic sequencing is that the remains did not show any evidence of admixture with European, Native American nor African individuals.  More than 97% of their genome fits exactly with the Polynesian motifs.  In other words, they appear to be first generation Polynesians.  They carry Polynesian mitochondrial, Y and autosomal (nuclear) DNA, exclusively.

Botocudo not admixed

In total, 25 Botocudo remains have been analyzed and of those, two have Polynesian ancestry and those two, BOT15 and BOT17, have exclusively Polynesian ancestry as indicated in the graphic above from the paper.

When did they live?  Accelerator mass spectrometry radiocarbon dating with marine correction gives us dates of 1479-1708 AD and 1730-1804 for specimen BOT15 and 1496-1842 for BOT17.

The paper goes on to discuss four possible scenarios for how this situation occurred and the pros and cons of each.

The Polynesian Peru Slave Trade

This occurred between 1862-1864 and can be ruled out because the dates for the skulls predate this trade period, significantly.

The Madagascar-Brazil Slave Trade

The researchers state that Madagascar is known to have been peopled by Southeast Asians and not by Polynesians.  Another factor excluding this option is that it’s known that the Malagasy ancestors admixed with African populations prior to the slave trade.  No such ancestry was detected in the samples, so these individuals were not brought as a result of the Madagascar-Brazil slave trade – contrary to what has been erroneously inferred and concluded.

Voyaging on European Ships as Crew, Passengers or StowAways

Trade on Euroamerican ships in the Pacific only began after 1760 AD and by 1760, Bot15 and Bot17 were already deceased with a probability of .92 and .81, respectively, making this scenario unlikely, but not entirely impossible.

Polynesian Voyaging

Polynesian ancestors originated from East Asia and migrated eastwards, interacting with New Guineans before colonizing the Pacific.  These people did colonize the Pacific, as unlikely as it seems, traveling thousands of miles, reaching New Zealand, Hawaii and Easter Island between 1200 and 1300 AD.  Clearly they did not reach Brazil in this timeframe, at least not as related to these skeletal remains, but that does not preclude a later voyage.

Of the four options, the first two appear to be firmly eliminated which leaves only the second two options.

One of the puzzling aspects of this analysis it the “pure” Polynesian genome, eliminating admixture which precludes earlier arrival.

The second puzzling aspect is how the individuals, and there were at least two, came to find themselves in Minas Gerais, Brazil, and why we have not found this type of DNA on the more likely western coastal areas of South America.

Minas Gerais Brazil

Regardless of how they arrived, they did, and now we know at least a little more of their story.

GedMatch

At GedMatch, it’s interesting to view the results of the one-to-one matching.

Both kits have several matches.  At 5cM and 500 SNPs, kit F999963 has 86 matches.  Of those, the mitochondrial haplogroup distribution is overwhelmingly haplogroup B, specifically B4a1a1 with a couple of interesting haplogroup Ms.

F999963 mito

Y haplogroups are primarily C2, C3 and O.   C3 and O are found exclusively in Asia – meaning they are not Native.

F999963 Y

Kit F999963 matches a couple of people at over 30cM with a generation match estimate just under 5 generations.  Clearly, this isn’t possible given that this person had died by about 1760, according to the paper, which is 255 years or about 8.5-10 generations ago, but it says something about the staying power of DNA segments and probably about endogamy and a very limited gene pool as well.  All matches over 15cM are shown below.

F999963 largest

Kit F999964 matches 97 people, many who are different people that kit F999963 matched.  So these ancient Polynesian people,  F999963 and F999964 don’t appear to be immediate relatives.

F999964 mito

Again, a lot of haplogroup B mitochondrial DNA, but less haplogroup C Y DNA and no haplogroup O individuals.

F999964 Y

Kit F999964 doesn’t match anyone quite as closely as kit F999963 did in terms of total cM, but the largest segment is 12cM, so the generational estimate is still at 4.6,  All matches over 15cM are shown below.

F999964 largest

Who are these individuals that these ancient kits are matching?  Many of these individuals know each other because they are of Hawaiian or Polynesian heritage and have already been working together.  Several of the Hawaiian folks are upwards of 80%, one at 94% and one believed to be 100% Hawaiian.  Some of these matches are to Maori, a Polynesian people from New Zealand, with one believed to be 100% Maori in addition to several admixed Maori.  So obviously, these ancient remains are matching contemporary people with Polynesian ancestry.

The Unasked Question

Sooner or later, we as a community are going to have to face the question of exactly what is Native or aboriginal.  In this case, because we do have the definitive autosomal full genome testing that eliminates admixture, these two individuals are clearly NOT Native.  Without full genomic testing, we would have never known.

But what if they had arrived 200 years earlier, around 1500 AD, one way or another, possibly on an early European ship, and had intermixed with the Native people for 10 generations?  What if they carried a Polynesian mitochondrial (or Y) DNA motif, but they were nearly entirely Native, or so much Native that the Polynesian could no longer be found autosomally?  Are they Native?  Is their mitochondrial or Y DNA now also considered to be Native?  Or is it still Polynesian?  Is it Polynesian if it’s found in the Cook Islands or on Hawaii and Native if found in South America?  How would we differentiate?

What if they arrived, not in 1500 AD, but about the year 500 AD, or 1000 BCE or 2000 BCE or 3000 BCE – after the Native people from Asia arrived but unquestionably before European contact?  Does that make a difference in how we classify their DNA?

We don’t have to answer this yet today, but something tells me that we will, sooner or later…and we might want to start pondering the question.

Acknowledgements: 

I want to thank all of the people involved whose individual work makes this type of comparative analysis possible.  After all, the power of genetic genealogy, contemporary or ancient, is in collaboration.  Without sharing, we have nothing. We learn nothing.  We make no progress.

In addition to the various scientists and papers already noted, special thanks to Felix Immanual for preparing and uploading the ancient files.  This is no small task and the files often take a month of prep each.  Thanks to Kalani for bringing this to my attention.  Thanks to Ray Banks for his untiring work with haplogroup C and for maintaining his haplogroup webpage with specifics about where the various subgroups are found.  Thanks to ISOGG’s volunteers for the haplotree.  Thanks to GedMatch for providing this wonderful platform and tools.  Thanks to everyone who uploads their DNA, and that of their relatives and works on specific types of projects – like Hawaiian and Maori.  Thanks to my haplogroup C-P39 co-administrators, Dr. David Pike and Marie Rundquist, for their contributions to this discussion and for working together on the Native American Haplogroup C-P39 Project.  It’s important to have other people who are passionate about the same subjects to bounce things off of and to work with.  This is the perfect example of the power of collaboration!

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

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Kennewick Man is Native American

Finally, an answer, after almost 20 years and very nearly losing the opportunity of ever knowing.

Today, in Nature, a team of scientists released information about the full genomic sequencing of Kennewick Man who was discovered in 1996 in Washington state.  Previous DNA sequencing attempts had failed, and 8000 year old Kennewick Man was then embroiled in years of legal battles.  Ironically, the only reason DNA testing was allowed is because, based on cranial morphology it was determined that he was likely more closely associated with Asian people or the Auni than the Native American population, and therefore NAGPRA did not apply.  However, subsequent DNA testing has removed all question about Kennewick Man’s history.  He truly is the Ancient One.

Kennewick man is Native American.  His Y haplogroup is Q-M3 and his mitochondrial DNA is X2a.  This autosomal DNA was analyzed as well, and compared to some current tribes, where available.

From the paper:

We find that Kennewick Man is closer to modern Native Americans than to any other population worldwide. Among the Native American groups for whom genome-wide data are available for comparison, several seem to be descended from a population closely related to that of Kennewick Man, including the Confederated Tribes of the Colville Reservation (Colville), one of the five tribes claiming Kennewick Man. We revisit the cranial analyses and find that, as opposed to genomic-wide comparisons, it is not possible on that basis to affiliate Kennewick Man to specific contemporary groups. We therefore conclude based on genetic comparisons that Kennewick Man shows continuity with Native North Americans over at least the last eight millennia.

Interestingly enough, the Colville Tribe, located near where Kennewick Man was found, decided to participate in the testing by submitting DNA for comparison.

Kennewick Colville

The ancestry and affiliations of Kennewick Man by Rasmussen, et al, Nature (2015) doi:10.1038/nature14625

Also from the paper:

Our results are in agreement with a basal divergence of Northern and Central/Southern Native American lineages as suggested from the analysis of the Anzick-1 genome12. However, the genetic affinities of Kennewick Man reveal additional complexity in the population history of the Northern lineage. The finding that Kennewick is more closely related to Southern than many Northern Native Americans (Extended Data Fig. 4) suggests the presence of an additional Northern lineage that diverged from the common ancestral population of Anzick-1 and Southern Native Americans (Fig. 3). This branch would include both Colville and other tribes of the Pacific Northwest such as the Stswecem’c, who also appear symmetric to Kennewick with Southern Native Americans (Extended Data Fig. 4). We also find evidence for additional gene flow into the Pacific Northwest related to Asian populations (Extended Data Fig. 5), which is likely to post-date Kennewick Man. We note that this gene flow could originate from within the Americas, for example in association with the migration of paleo-Eskimos or Inuit ancestors within the past 5 thousand years25, or the gene flow could be post colonial19.

The authors go on to say that Kennewick Man is significiantly different than Anzick Child, which matches closely with many Meso and South American samples.  Kennewick on the other hand, is closely related to the Chippewa and Anzick was not.

This divergence may suggest a population substructure and migration path within the Americas, although I would think significantly more testing of Native people would be in order before a migration path would be able to be determined or even suggested. It is very interesting that Anzick from Montana, 12,500 years ago, would match Meso American samples so closely.  I would have expected Kennewick to perhaps match Meso Americans more closely because I would have expected the migration pathway to be down the coastline.  Perhaps that migration had already happened by the time Kennewick man came onto the scene some 8000 years ago.

You can read the entire paper at this link.

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Yamnaya, Light Skinned, Brown Eyed….Ancestors???

Late last fall, I reported that scientists had discovered a European ghost population.  This group of people then referred to as the ANE, Ancient Northern Europeans, was a previously unknown population from the north that had mixed into the known European populations, the Hunter-Gatherers and the farmers from the Middle East, the Neolithic.

That discovery came as a result of the full genome sequencing of a few ancient specimens, including one from the Altai.

Recently, several papers have been published as a result of ongoing sequencing efforts of another 200 or so ancient specimens.  As a result, scientists now believe that this ghost population has been identified as the Yamnaya and that they began a mass migration in different directions, including Europe, about 5,000 years ago.  Along with their light skin and brown eyes, they brought along with them their gene(s) for lactose tolerance.  So, if you have European heritage and are lactose tolerant, then maybe you can thank your Yamnaya ancestors.

1.Haak et al. http://doi.org/z9d (2015) from Feb. 18, 2015 “Steppe migration rekindles debate on language origin” by Ellen Callaway

1.Haak et al. http://doi.org/z9d (2015) from Feb. 18, 2015 “Steppe migration rekindles debate on language origin” by Ellen Callaway

For those of us who avidly follow these types of discoveries, this is not only amazing, it’s wonderful news.  It helps to continue to explain how and why some haplogroups are found in the Native American population and in the Northern European population as well.  For example, haplogroup Q is found in both places – not exact duplicates, but certainly close enough for us to know they were at one time related.  It also explains how people from Germany, for example, are showing small percentages of Native American ancestry.  Their common ancestors were indeed from central Asia, thousands of years ago, and we can still see vestiges of that population today in both groups of people.

So, if the Yamnaya people are the ghost people, the ANE, who are they?

The Yamna culture was primarily nomadic and was found in Russia in the Ural Region, the Pontic Steppe, dating to the 36th-23rd century BC.  It is also known as the Pit Grave Culture, the Ochre Grave Culture and feeds into the Corded Ware Culture.

"Corded Ware culture" by User:Dbachmann - Own work based based on Image:Europe 34 62 -12 54 blank map.png. Licensed under CC BY-SA 3.0 via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Corded_Ware_culture.png#/media/File:Corded_Ware_culture.png

“Corded Ware culture” by User:Dbachmann – Own work based based on Image:Europe 34 62 -12 54 blank map.png. Licensed under CC BY-SA 3.0 via Wikimedia Commons – https://commons.wikimedia.org/wiki/File:Corded_Ware_culture.png#/media/File:Corded_Ware_culture.png

Characteristics for the culture are burials in kurgans (tumuli) in pit graves with the dead body placed in a supine position with bent knees. The bodies were covered in ochre. Multiple graves have been found in these kurgans, often as later insertions.  The first known cart burial is also found in a kurgan grave.  A kurgan often appears as a hill, example shown below, and have been found in locations throughout eastern and northern Europe..

Hallstatt-era tumulus in the Sulm valley necropolis in Austria, photo by Hermann A. M. Mucke.

Hallstatt-era tumulus in the Sulm valley necropolis in Austria, photo by Hermann A. M. Mucke.

Additionally, some scientists believe that the Yamna culture was responsible for the introduction of PIE, Proto-Indo-European-Language, the now defunct mother-tongue of European languages.  Others think it’s way too soon to tell, and that suggestion is jumping the gun a bit.

Why might these recent discoveries be important to many genetic genealogists?  Primarily, because Y haplogroup R has been identified in ancient Russian remains dating from 2700-3400 BCE.  Haplogroup R and subgroups had not been found in the ancient European remains sequenced as of last fall.  In addition, subgroups of mitochondrial haplogroups U, W, H, T and W have been identified as well.

Keep in mind that we are still dealing with less than 300 skeletal remains that have been fully sequenced.  This trend may hold, or a new discovery may well cause the thought pattern to be “reconfigured” slightly or significantly.  Regardless, it’s exciting to be part of the learning and discovery process.

Oh yes, and before I forget to mention it…it seems that your Neanderthal ancestors may not be as far back in your tree as you thought.  They have now found 40,000 year old skeletal remains that suggest that person’s great-great-grandfather was in fact, full Neanderthal.  That’s significantly later than previously thought, by 10,000 or 20,000 years, and in Europe, not the Near East…and who knows what is just waiting to be found.  The new field of ancient DNA is literally bursting open as we watch.

I’ve accumulated several recent articles and some abstracts so that you can read about these interesting developments, in summary, and not have to do a lot of searching.  Enjoy!

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Modern Europe was formed by milk-drinking Russians: Mass migration brought new genetic makeup to continent 5,000 years ago
http://www.dailymail.co.uk/news/article-3119310/How-white-Europeans-arrived-5-000-years-ago-Mass-migration-southern-Russia-brought-new-technology-dairy-farming-continent.html

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DNA Deciphers Roots of Modern Europeans
http://www.nytimes.com/2015/06/16/science/dna-deciphers-roots-of-modern-europeans.html?smid=fb-nytimes&smtyp=cur&_r=1

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Science – Nomadic Herders Left a Strong Genetic Mark on Europeans and Asians
http://news.sciencemag.org/archaeology/2015/06/nomadic-herders-left-strong-genetic-mark-europeans-and-asians

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Nature – DNA Data Explosion Light Up the Bronze Age
http://www.nature.com/news/dna-data-explosion-lights-up-the-bronze-age-1.17723

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From the European Nucleotide Archive.  http://www.ebi.ac.uk/ena/data/view/PRJEB9021

Investigation of Bronze Age in Eurasia by sequencing from 101 ancient human remains. We show that around 3 ka BC, Central and Northern Europe and Central Asia receive genetic input through people related to the Yamnaya Culture from the Pontic-Caspian Steppe, resulting in the formation of the Corded Ware Culture in Europe and the Afanasievo Culture in Central Asia. A thousand years later, genetic input from North-Central Europe into Central Asia gives rise to the Sintashta and Andronovo Cultures. During the late BA and Iron Age, the European-derived populations in Asia are gradually replaced by multi-ethnic cultures, of which some relate to contemporary Asian groups, while others share recent ancestry with Native American

Description

The Bronze Age (BA) of Eurasia (c. 3,000-1,000 years BC, 3-1 ka BC) was a period of major cultural changes. Earlier hunter-gathering and farming cultures in Europe and Asia were replaced by cultures associated with completely new perceptions and technologies inspired by early urban civilization. It remains debated if these cultural shifts simply represented the circulation of ideas or resulted from large-scale human migrations, potentially also facilitating the spread of Indo-European languages and certain phenotypic traits. To investigate this and the role of BA in the formation of Eurasian genetic structure, we used new methodological improvements to sequence low coverage genomes from 101 ancient humans (19 > 1X average depth) covering 3 ka BC to 600 AD from across Eurasia. We show that around 3 ka BC, Central and Northern Europe and Central Asia receive genetic input through people related to the Yamnaya Culture from the Pontic-Caspian Steppe, resulting in the formation of the Corded Ware Culture in Europe and the Afanasievo Culture in Central Asia. A thousand years later, genetic input from North-Central Europe into Central Asia gives rise to the Sintashta and Andronovo Cultures. During the late BA and Iron Age, the European-derived populations in Asia are gradually replaced by multi-ethnic cultures, of which some relate to contemporary Asian groups, while others share recent ancestry with Native Americans. Our findings are consistent with the hypothesised spread of Indo-European languages during early BA and reveal that major parts of the demographic structure of present-day Eurasian populations were shaped during this period. We also demonstrate that light skin pigmentation in Europeans was already present at high frequency during the BA, contrary to lactose tolerance, indicating a more recent onset of positive selection in the latter than previously believed.

Abstract

The Bronze Age (BA) of Eurasia (c. 3,000-1,000 years BC, 3-1 ka BC) was a period of major cultural changes. Earlier hunter-gathering and farming cultures in Europe and Asia were replaced by cultures associated with completely new perceptions and technologies inspired by early urban civilization. It remains debated if these cultural shifts simply represented the circulation of ideas or resulted from large-scale human migrations, potentially also facilitating the spread of Indo-European languages and certain phenotypic traits. To investigate this and the role of BA in the formation of Eurasian genetic structure, we used new methodological improvements to sequence low coverage genomes from 101 ancient humans (19 > 1X average depth) covering 3 ka BC to 600 AD from across Eurasia. We show that around 3 ka BC, Central and Northern Europe and Central Asia receive genetic input through people related to the Yamnaya Culture from the Pontic-Caspian Steppe, resulting in the formation of the Corded Ware Culture in Europe and the Afanasievo Culture in Central Asia. A thousand years later, genetic input from North-Central Europe into Central Asia gives rise to the Sintashta and Andronovo Cultures. During the late BA and Iron Age, the European-derived populations in Asia are gradually replaced by multi-ethnic cultures, of which some relate to contemporary Asian groups, while others share recent ancestry with Native Americans. Our findings are consistent with the hypothesised spread of Indo-European languages during early BA and reveal that major parts of the demographic structure of present-day Eurasian populations were shaped during this period. We also demonstrate that light skin pigmentation in Europeans was already present at high frequency during the BA, contrary to lactose tolerance, indicating a more recent onset of positive selection in the latter than previously believed.

The findings echo those of a team that sequenced 69 ancient Europeans3. Both groups speculate that the Yamnaya migration was at least partly responsible for the spread of the Indo-European languages into Western Europe.

The report on the 69 ancient remains sequenced is below.

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Steppe migration rekindles debate on language origin
http://www.nature.com/news/steppe-migration-rekindles-debate-on-language-origin-1.16935

The Harvard team collected DNA from 69 human remains dating back 8,000 years and cataloged the genetic variations at almost 400,000 different points. The Copenhagen team collected DNA from 101 skeletons dating back about 3,400 years and sequenced the entire genomes.

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Population genetics of Bronze Age Eurasia
http://www.nature.com/nature/journal/v522/n7555/full/nature14507.html

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Dienekes Anthropology Blog
http://dienekes.blogspot.com/2014/06/ancient-dna-from-bronze-age-altai.html

Forensic Science International: Genetics Received 2 January 2014; received in revised form 21 May 2014; accepted 25 May 2014. published online 04 June 2014.

The Altai Mountains have been a long term boundary zone between the Eurasian Steppe populations and South and East Asian populations. Mitochondrial DNA analyses revealed that the ancient Altaians studied carried both Western (H, U, T) and Eastern (A, C, D) Eurasian lineages. In the same way, the patrilineal gene pool revealed the presence of different haplogroups (Q1a2a1-L54, R1a1a1b2-Z93 and C), probably marking different origins for the male paternal lineages.

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Dienekes Anthropology Blog
http://dienekes.blogspot.com/2013/06/mtdna-from-late-bronze-age-west-siberia.html

Includes mitochondrial haplogroups C, U2e, T, U5a, T1, A10.

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Population Genetics copper and Bronze Age populations of Eastern Steppe, thesis by Sandra Wilde
http://ubm.opus.hbz-nrw.de/volltexte/2015/3975/ (in German)

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Eurogenes blog discusses
http://eurogenes.blogspot.com/2015/03/population-genetics-of-copper-and.html

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Polish Genes Blog
http://polishgenes.blogspot.com/2015/05/r1a1a-from-early-bronze-age-warrior.html

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Early European May Have Had Neanderthal Great-Great-Greandparent
http://www.nature.com/news/early-european-may-have-had-neanderthal-great-great-grandparent-1.17534

40,000 year old Romanian skeleton with 5 – 11% Neanderthal, including large parts of some chromosomes – as close as a great-grandparent.  Previously thought that interbreeding was in the Middle East and 10,000 or 20,000 years earlier.

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How is this all happening?

The Scientist Magazine has a great overview in the June 1, 2015 edition, in “What’s Old is New Again.”
http://www.the-scientist.com/?articles.view/articleNo/43069/title/What-s-Old-Is-New-Again/

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George Estes (1763-1859), 3 Times Revolutionary War Veteran, 52 Ancestors #66

George Estes was born in Amelia County, Virginia to Moses Estes and Luremia Combs on February 3, 1763.  He tells us his birth date and his father’s name, among several other very interesting things, in his application for a Revolutionary War pension.

In 1832, Congress passed an act making men who served in the Revolution eligible for a pension.  Thank goodness they did, because it caused records to be created documenting the service and lives of these men that would otherwise never have existed.

George applied for his pension on September 14, 1833.  In his owns words, he tells us about his 3 tours of duty.  Yes, three separate tours of duty.

George Estes pension

George Estes pension 2

George Estes pension 3

“I entered the service in January 1781 as a substitute for my father Moses Estes and marched from Halifax County where I then lived under Captain Wall through the counties of Charlotte, Lunenburg, Dinwiddie and Petersburg to Cabin Point on the James River.”

I would think the word “marched” implies on foot.  And there is a lot of marching going on.

Cabin point

“At that place I was transferred to Capt. Long’s company of infantry and marched with him to Suffolk on the Nansemond River where I was stationed for some time under Colonel Dick and Gen. Michlenburg.  From there we marched to Portsmouth and many other places and arrived at Barrett’s Neck where I was discharged by Capt. Lewis in the month of April 1781 having served 3 months on this tour.  My discharge is lost and cannot be found but the service record is proved by Elias Palmer who was a soldier with me during the whole time.

In the month of May 1781 I was drafted to serve my own tour and marched from Halifax County in Capt. Clark’s company through Richmond to New Kent Courthouse where we joined General Mechlenburg’s Company.  I was then attached to Capt. Read’s company of cavalry and continued with him marching in various directions until our time of service for 3 months expired.  I was discharged by General Waine in the County of Charles City in the month of August 1781.  My discharge is lost and cannot be found and I do not know any person living who was in that service with me.

In the fall of 1781 I moved a family of people to the state of Tennessee staid in that country upwards of a year and in the month of October 1782 I entered the service of the United States as a volunteer and marched from the county of Washington in state of North Carolina in Capt. Cox’s company of mounted horsemen under Col. Campbell and Col. Shelby into the Cherokee Nation of Indians.  We marched in various directions in the said nation until we arrived at the shoemake town.  At that place we received information that a treaty had been reached with the indians and we were discharged.  The whole time of service on this term was 2 months and 20 days.  I was discharged by Capt. Cox about the end of December and came to Virginia where I have lived in the county of Halifax ever since.  My discharge is lost and cannot be found and no person [is] in this country that was in that service with me.

I was born in the county of Amelia on the third day of February 1763.  My age was recorded in a family bible that was in my father’s possession but I don’t know what became of it.  I lived in the county of Halifax when I entered the service in the said county when the war ended and have lived in the county ever since.  Christopher White, Thomas Conner and Peter F. Kent and many others can testify as to my character for veracity and their belief of my service as a soldier of the Revolution.  There is no clergyman living in my neighborhood.”

George (X) Estes

      (his mark)

Sept. 14, 1833

So George served three times in total, twice by obligation, when his father and his numbers came due, and once as a volunteer.  He served in place of his father.  War is difficult enough for a young man.

When George filed for his pension, he was 70 years old.  While he signed with an X in 1833, in earlier documents, he signed his name, so he was capable of writing.

George Estes signature 1

George’s signature is shown on a petition dated Dec. 10, 1785, above, for an assessment for religious teachers.  Note that his name appears very near that of William Younger who lived adjacent his father Moses Estes.  George would marry Mary Younger a year later, although a connection between the two Younger families has never been proven.

George Estes signature 2

This petition dated November 17, 1795 shows Moses and his son George Estes both of whom are opposed to the sale of the church glebe lands, in addition to the signature of their neighbor William Younger.  Note that George actually spells his own name in two different ways, Estes and Eastis.  And we wonder why we are confused today.

Documenting George’s first two tour service records in Virginia was difficult, but finding the third one was next to impossible.  Then, quite by accident, when looking for my Dodson family records, I stumbled across the documentation for George’s third tour, where he is listed as George Eastis, in the North Carolina archives, of all places.

From the Book “Tennessee Soldiers in the Revolution” by Penelope Johnson Allen, now digitized at Ancestry.com.

George Estes rev war accounts

Look at this, George is right across the page, directly from Lazarus Dodson, the man I was looking for.  Talk about serendipity.

George Estes Army account

My cousin, Debbie, wrote to the NC archives and was sent the following document that tells us that George Estes was paid in a specie certificate, a type of credit voucher, on June 12, 1783.  His name appears on the 10th line in the third column.  Ironically, Lazarus Dodson, whose name appears two entries below George’s is the father of Lazarus Dodson, whose daughter, Rutha or Ruthy, would marry George’s grandson John Y. Estes in Claiborne County, Tennessee in January 1841, 58 years after their grandfathers  served together in the Revolutionary War.  I wonder if they ever figured that out.

George Estes specie certificates

I called the North Carolina archives and asked if the original pay rosters and additional information were available.  They said they were, but they did not do “lookup work.”  A week later, I was standing at the research desk in the archives in Raleigh, with these papers in hand, and an amazed librarian kind of stuttered and stammered around when I introduced myself and told her where I came from (Michigan) and why I was there.  I think they are far more used to people “going away” when told the archives doesn’t do “lookup work” than showing up 1000 miles and a week later.  Sadly, that trip was for naught, because while they did have additional records for some soldiers, there was nothing more for George.  Don’t even ask how upset I was.

Why, I was then forced to do research on some of my NC lines since I was there in the archives with nothing else to do.  I mean…you can’t waste a trip like that!

George’s certificate was issued by the auditors, Bledsoe and Williams, and by referencing the attached documents, you can determine the location where the soldiers served. In this case, exactly as described by George Estes, he served in the Morgan District which included the Washington and Sullivan County areas which eventually became Tennessee.

George Estes army districts

By putting these three pieces of information together, George’s pay list, which includes the auditor, the auditor and their districts – we can confirm where George was when he served his third service term.

George Estes district auditors

In 1833, from Jasper Co., GA, Clarissa C. Boyd declares that her brother, George Easters, a resident of Halifax Co., VA in 1781, served 6 months in the Virginia militia. On January 15, 1784. George Estes, infantry, Continental Line, was issued a certificate for the balance of his pay.

George was placed on Virginia pension roll at $31.38 per annum, certificate 16886 issued on Oct. 12, 1833.

On April 5, 1855 in Halifax Co., George (X) Estes of said county, age 92, applies for bounty land.  He obtains the land and signs the bounty certificate over to his daughter Susannah immediately.

What do we know about what happened to George during his Revolutionary War service?

In his first term of service, serving in place of his father, Moses, George spent time at Cabin Point on the James River about which we discover the following:

By late summer 1780 with South Carolina under their control, the British were ready to push into Virginia and Maryland and deal Washington a final blow. In Virginia, Governor Thomas Jefferson had placed General Steuben in charge of the state’s defense. By January 1, 1781, the British were in Chesapeake Bay and Jefferson was calling up county militiamen to repel the impending attack. Benedict Arnold, now in charge of the British fleet, sailed up the James River and burned Richmond then moved back downriver to settle in at Portsmouth on the Chesapeake Bay.  The Halifax County Militia and was sent to Cabin Point on the James River to watch for Arnold’s next anticipated raid up the river. The militia had little to do but sit and wait and worry about the news coming in daily of Cornwallis’ raids in the Carolinas and his impending threat to Virginia.

It seems that all was not well at home in Halifax County during this time.  Boyd’s Ferry is the present city of South Boston and the Boyd’s Ferry crossing was very close to the Estes homestead, which was located just above the crossing on the main road.

In a letter to Governor Jefferson dated February 15th, 1781, camped at Boyd’s Ferry on the Dan River, Greene called for reinforcement of militia:

“We have crossed the Dan, and I am apprehensive they will cross it above us…If they should they will oblige us to cross the Stanton branch of the Roanoke…It is by no means certain, that Lord Cornwallis will not push through Virginia.”

Jefferson dispatched letters on February 17 and 18 to a long list of county Lieutenants and Baron von Steuben asking for militia to join General Greene who had “crossed the Dan at Boyd’s Ferry and was retreating before the enemy.” News of the alarming activities of Greene and Cornwallis aligned along either side of the Dan near Boyd’s Ferry must have reached the Halifax County Militiamen shortly after February 18. While they sat on the James River waiting for Arnold to make a move, Cornwallis and his army was camped at the doorstep of their homes in Halifax County.

The record is dated February 23, 1781 Cabin Point, Virginia and states:

“A list of the mens names belonging to Major Jones Battalion of Militia who have deserted. Distinguishing those who carried off their arms from those who did not. Also those who deserted from their post.”

The list of names does not include George Estes.  He had a decision to make, and he chose to remain at his post, although one could scarcely have blamed him had he returned home to protect and defend his home place and family.  Perhaps the knowledge that his father and siblings were there relieved his mind somewhat.

Now let’s turn to George’s third tour of duty from what would become eastern Tennessee, but was at that time western North Carolina..

In 1782, the Cherokee, who had sided with the British continued to raid.  John Sevier banded together a group of men in western North Carolina, now eastern Tennessee, and with Colonels Campbell and Shelby marched on the Cherokee towns.  Shoemake town, as it was called by whites, was located in upper Georgia and had previously been burned in May of 1781.  The Indians allied with the British because the British assured them that they would stop the encroachment of the Europeans into their traditional territory.  The Indians did not fare well in the Revolutionary War, nor afterwards.  This “march on the Cherokee” appears to have been one last final grandstand that gave the Cherokee the final nudge to end their part in the war.

Overhill towns map

Rather miraculously, George does not seem to have engaged in any actual battles during his 3 tours of duty.  By this late date in the war, most of the actual fighting was in North and South Carolina.

Back Home in Halifax County

George Estes street sign

After returning to Halifax County, George Estes spent most of his life on his father’s original land.  His father Moses died in 1813, but the estate was contested and not settled until 1837, long after many of Moses’s children had died as well.

That land is located in the city of South Boston at the intersection of Estes and Main Street.  The following photo is standing in the Oak Ridge cemetery, originally part of the Estes land, looking down Estes Street.  Note the blue water tower.  It’s a landmark we’ll reference later.

George Estes land

The Estes farm used to be beyond the blue tank on the left and the houses on the right. Today Estes Street is gated, not because it’s an upscale gated community, but because that land is now the landfill.  This was heartbreaking to me, until I learned that the graves had been moved.  It still makes me sad.

Below is what’s left of the Estes land taken from behind the area (yes, I was in the landfill but I cropped that portion from the photo.)  We are looking at the original Estes woods.

George Estes landfill

In the above photo, for perspective, notice the blue water tower in the upper right corner. In the photo below, you can see the ‘other end” of the now gated “Estes Street” emerging that originates near the blue water tower that can also be seen in the left upper corner of the photo.

George Estes landfill 2

The Estes family in Halifax County, Virginia tells the story of when the family moved the graves from the old Estes land shown above to the Estes plot in the Oak Ridge cemetery. This apparently happened in the early 1900s and the only graves not moved were those of two unrelated people, one being an unrelated child whose parents had no place to bury the child and the second, an “in-law” of a descendant whose family did not want them moved.

It turns out that when Moses Estes’ children fought so bitterly over his land, they also apparently established separate cemeteries. One cemetery was the “original” Estes cemetery where Ezekiel, Susannah,  Ezekiel’s mother who is George’s daughter, George and probably old Moses himself are buried. The other cemetery was located behind the houses, apparently, down Estes street. I believe that the Oak Ridge Estes plot is the original Estes cemetery, but I cannot definitively prove this through records still in existence today, although an early cemetery history states that this is the case. Oral history says that when they moved Moses’s grave, only a collar bone and a casket hinge were left. Whether this is accurate or a tall tale, we’ll never know, but indeed, whatever remains of the elder Estes clan is buried in the Oak Ridge cemetery directly across the street from the old Estes homestead and at the end of Estes Street. The rest, well, it’s under the landfill or dispersed.

Today Main Street is paved. When they removed the cobblestones to pave Main Street, they used them to construct the beautiful stone wall around the cemetery. George Estes served on many “road crews” as documented in court records and it is entirely possible that he laid these very cobblestones, shaped from the stones found on the Estes land. George was probably glad to get rid of them as they would have made plowing difficult.

The bright white monuments in the cemetery are the Estes family stones, made of marble apparently, after they were cleaned by family members about 2006. Ezekiel who died in 1885 has a stone that proclaims him “an honorable man,” but none of the earlier family members have stones. Ezekiel’s mother Susannah died in 1870 and his grandfather George died in July of 1859, an amazing 96 years of age.

Oak Ridge cem entrance

The Halifax County Estes family has a clearly remembered oral history of “Granpappy George who lived to be 108 (or 106 or 115).” Sometimes stories grow with time, and that one certainly did, but he was quite elderly when he passed and obviously legendary.

George lived far from a sedentary lifestyle. He was obviously not afraid of adventure or danger, serving three separate terms in the Revolutionary War, one as a substitute for his father and one as a volunteer. George returned home and married Mary Younger on December 19, 1786 the same day that his brother Bartlett Estes married Rachel Pounds. I wonder if they were married in a double ceremony.

estes younger marriage

Younger marcus signature

When I first started researching this couple, everyone in the family said that George Estes and Mary Younger could not have been the father of John R. Estes because they only had one child, Susannah. As a novice, I figured those researchers had a lot more information and years of experience, but as one by one, I worked through and eliminated many of the alternative parents, the options became fewer and fewer and I began to wonder how “they” knew that George only had one child. I certainly hadn’t found anything that said he had only one child. And having found only one child doesn’t mean there was only one child. In fact, I’ve become very suspicious of any record before the days of modern birth control that suggests that someone had only one or two children, unless the wife or husband died.

As it turns out, Susannah was the only child that was easily evident. And “they” didn’t know how “they” knew – trying to find the source of that information was like trying to find the elusive fountain of youth. And that was before the days of quick-click trees on Ancestry. If the researchers had looked at the few census records we do have, they would have seen a discrepancy that screamed for an explanation – multiple children living with George and Mary.

George and Mary positively had 7 children who survived to adulthood and probably at least two who didn’t, based on a combination of records, including the 1820 and 1830 census.

It seems that several of George’s children regularly pushed the envelope of the day and would have brushes with the law or, perhaps better stated, the court system and “polite society.” It’s thanks to those records that we can add color to our family portrait. I love lawsuits – well – historical lawsuits anyway. I extracted probably 75-100 years worth of court, deed and tax records from Halifax County and reassembled them, like a big puzzle, into family groups.

Of particular interest was the information from the “Younger, Marcus Chancery Suit 1842-057, Halifax Co. Va.” In the documents from that suit, I found the payments made to the various heirs of Marcus Younger, who had died in 1816. In the case of Mary Younger Estes, her heirs are listed in 1842 because she is deceased. This suit was filed almost 30 years after Marcus’s death.  Normally would never think to look that far out – but chancery suits are often quite different. It’s not at all unusual for chancery suits to reach back 2 generations, to a grandparent’s will, especially if unmarried children are involved, as was the case with Marcus’s will. When the unmarried child dies, Mary’s sister in this case, sometimes the assets revert to the other children or their heirs.

In the suit papers, it is noted that Mary Younger Estes’s children will receive one sixth of her one quarter share of the 83 acres to be sold following the death of Mary’s unmarried sister.

The children of Mary Younger Estes were listed as: John, William, Susannah, Sally wife of T. Estes, Polly wife of James Smith and a grandchild name Mark Estes. This means that Mary had 6 children either living or who had died but who have heirs. In this case, one child has died leaving one heir, Mark.

Of course, I found this list AFTER I had reassembled what I believed to be the family of Mary Younger and George Estes. You know it didn’t match up perfectly, or I wouldn’t even be mentioning it.

I had all of those children listed, but in addition, I had a Bartlett and Rebecca.

There is no son Bartlett listed in the 1842 document, but there is instead a grandchild named Mark Estes. This implies that Mark’s parent is of the Estes surname, the parent is dead and Mark is the only living child. We know through various records that daughter Susannah has a son, Mark, but this cannot be that Mark because Susannah is very clearly included as living. We also know that George’s son, Marcus, died in 1815 leaving a widow and no children. The Bartlett I have attributed as the son of Mary and George had 7 children, and none known to be Mark, although one male name is unknown.

There are several Bartletts living in this vicinity and I could have the various Bartlett’s confused. However, if daughter Rebecca died and left a son Mark, this would fit perfectly. But, if it is the same Rebecca, she is prosecuted in 1844 for living with a black man, which precludes her from being dead in 1842, so Rebecca is not the child of George Estes, but more likely George’s niece.

This family makes me pull my hair out.

Thankfully, it seems that several of George’s children have lived a bit of a colorful life, meaning they have records that remain about them having had brushes with the law or, perhaps better stated, the court system and running afoul of “polite society.” Here is what is known about the children of George Estes and Mary Younger.

  • John R. Estes whose photo we believe is shown below was probably the oldest child and was born sometime between March and June of 1787. He married Ann Moore in 1811 and was on the way to Claiborne Co., TN by 1820 where he died in 1885, like his father, nearly reaching 100 years of age. Obviously, there is a longevity gene in the Estes family. John R.’s great grandson, William George Estes lived to be 98 years of age and William George’s two daughter both lived to be just shy of 100.  There’s hope that I’ll live long enough to finish my genealogy research:)

John R. Estes restored

  • Marcus Estes was born about 1788 as well and is shown on tax lists in Halifax County from 1811-1814 when he dies, leaving a widow with the beautiful name of Quintinney. In 1815, his estate is assigned to the sheriff to administer. There is also a War of 1812 record for Marcus, but there may be no further service records since no one applied for either bounty land or a pension based on his service. He served in the same unit as his brother, John R. Estes and I have to wonder if he died during that time.
  • William Y. Estes was also born sometime in this timeframe. The census says 1785 or 1786, but the census is also often notoriously wrong. In 1815, William married Rebecca Miller and drank to the point where his wife’s father commented on his behavior in his will in a very unflattering manner, forbidding William to ever have any control over Rebecca’s inheritance. William died in Halifax County between 1860 and 1870.
  • Susannah Y. Estes was born about 1800 and never married. She had 5 illegitimate children between 1814 and 1835, 2 males and three females. She lived on the old home place and cared for her elderly father, George, until his death in 1859.
  • Polly Estes, born between 1801 and 1808 married in 1824 to James Smith. She died in Halifax County after 1880, having had 4 children. We know very little about Polly, because, she was apparently one of the few well-behaved Estes’s. You know that old saying about “well behaved women seldom make history.”  I relish my ill-behaved ancestors and their family members because that is often the only way we learn about their lives and put meat on their bones.  Below, George’s signature along with James Smith when Polly marries.

George Estes Polly marriage bond

This photo is of George’s grandchild, J. E. and wife Mary Anne Smith, the youngest child of Polly Estes Smith.

JE and Mary Ann Smith

I’m dying to know about that eye patch.

  • Sally Estes was born sometime around 1800 and married her first cousin, Thomas Estes, son of Bartlett Estes and Rachel Pounds. Marrying cousins was a common practice of the time. They removed to Tennessee shortly after their marriage.  George and Thomas both sign the marriage bond, below.

George Estes Sally marriage bond

I initially thought Rebecca Estes was George’s child because of her proximity in the census where in 1830, a Rebecca Estridge with 3 daughters is living near George Estes and Susan Estes, all living in separate households. In 1835, a Rebecca Estes is in the court notes with Robert Rickman for support of her child, and in 1844, Rebecca is “indicted for felony, report of grand jury – a white woman living together in open adultery with a negro man, James Bird, free man of color, as presented by Jacob W. Farguson and William Ingram.” I cannot find Rebecca nor James Bird after this time. If this is the same Rebecca in 1844 as in 1830 and 1835, then she cannot be the child of George Estes because in 1842, Rebecca would have been dead.

It’s very unlikely that either Bartlett or Rebecca are George’s children and we are simply missing one child who had son Mark. It is certainly possible that this Marcus was born posthumously to George’s son Marcus. Given that Marcus’s estate went entirely to debt, there would have been nothing left to leave to a child, so no guardian would have been appointed.  We’ll likely never know, but this is the most likely explanation.  There is no Mark or Marcus Estes in the 1840 or 1850 census.

Life in Halifax County with Daughter Susannah

We don’t have a lot of information about life as George knew it, but thanks to Susannah, we do have a couple of glimpses into what their life was like.

Susannah Estes never married, lived on the old homeplace and wound up with all of George’s assets which caused problems with his other children. By the time George Estes died in 1859, there was nothing left, so he had no will. He had already deeded his land to Susannah, plus anything left from his pension or his Revolutionary War service.

On February 12, 1833, George Estes grants to Susan Y. Eastes, “my daughter, all my right, title, claim and interest which I have for military services rendered during the War of the Revolution.”

Much to my shock, in early 1837, Susannah brings suit against her father forcing him to answer to the court why he, as executor, has not distributed his father, Moses’s estate.

On March 25, 1837, George Estes deeds to Susannah Y. Estes “for $100 land on both sides of road from Halifax to S. Boston on Dan River adjoining Adam Toot, John Ransom, John Jinnett, tract of land that my father Moses died seized of.” This occurs immediately after George’s father’s estate was settled.

If you look at a map of South Boston plotting the locations we know, this is a huge tract of land.

Estes land South Boston map

We know the land went as far north at present day Waddell Woods (top arrow) because Waddell spring is mentioned in deeds.  The Oak Ridge Cemetery is the green area pointed out by the second arrow from the top.  The blue water tower is across the street, to the right of that arrow.  The main road is 129 and is pointed to by the third arrow from the top, running from the Dan River (at the bottom) through the Estes land and on North.  Today, this land includes most of South Boston, then Boyd’s Ferry.

We get a glimpse of their possessions, when, in 1842, Susannah, who now owns her parents land, takes a mortgage which is void if it is paid. Apparently, the mortgage is paid, because nothing more is ever mentioned in any of the deed or court books.

“Tract of land where we now live, one three-horse wagon and gear, 1 bay mare, 1 grey horse, hogs and sheep, all of our present crop of corn and fodder, tobacco, 4 feather beds and furniture, household and kitchen furniture, plantation tools for debt of $50.16.”

In addition to the land George inherited from Moses, George continues to assist Susannah.

On April 15, 1857, George Estes deeds to Susan Y. Estes the bounty lands he is entitled to “by late acts of Congress and a part of proceeds being in the hands of Easley Holt and Co. In consideration of natural love and affection and value received…all right and interest to any balance that is remaining at my death after paying my debts with him.”

When she died on August 23, 1870, Susannah was not a poor woman and left a nontrivial estate, including land. Her personal property inventory probably included many items inherited from her father and mother:

Appraisement of property of Susan Estes:

  • cow
  • yearling
  • loom
  • potatoes
  • walnut chest
  • barrels
  • flax wheel
  • 3 pots
  • 2 skillets
  • oven
  • brass kettle
  • tea kettle
  • 4 jars
  • 4 jugs
  • 2 water buckets
  • 3 axes
  • lot tin
  • 2 pitchers and bottles
  • 1 jar vinegar
  • lot tableware
  • hoes
  • wedges
  • pot rack
  • candlesticks
  • 1 press
  • 1 desk
  • 1 looking glass
  • 7 chairs
  • 1 bed
  • bolster
  • pillar
  • 1 blanket
  • 1 counterpin and sheet
  • 1 quilt
  • 1 barrell cider
  • small chest
  • basket
  • 2 bee hives

I can’t help but wonder what the quilt looked like and who made it.  Was it from a time when she and her mother and sisters perhaps gathered around a quilting frame?

After Susan’s death, a lawsuit followed regarding a debt incurred before her death and the validity of the debt based on her mental state.  She was deemed competent.  Aside from the depositions, which were in themselves very enlightening as to Susannah’s life, and death, the list of items she purchased at the store, on account, I found very interesting as well:

The following are items appearing on the store account of “Miss Susan Estes”:

  • Coffee
  • Sugar
  • Bucket
  • Linen shirt
  • 2 linen collars
  • 5 yards calico (total 1.06)
  • 3 yard gingham
  • 1 bottle ? oil
  • 20 yards oznaburg
  • 75 yard pant goods
  • Weeding hoe
  • Shelves for buster
  • Coffee pot
  • Tin bucket
  • Sugar
  • Rice
  • Candles
  • Molasses
  • Coffee
  • Bacon
  • Molasses
  • Coffee
  • Nails
  • Shoes
  • 1 oz indigo
  • 1 # soda
  • Coffee
  • Sole leather
  • 2 oz indigo
  • Pale cotton
  • Sugar
  • Copperons?
  • Rubber tuck combs
  • 2 yd cambric
  • Flex thread
  • 6 8×10 window glass
  • Bacon
  • Seed oats
  • Bags
  • Frt and drayage
  • Paid on acct with bacon from house
  • Goods box
  • Plow point
  • Coffee
  • Fine iron
  • Goods box
  • Molasses
  • Hat for Buster
  • Pants for Buster
  • Coat for Buster
  • Vest for Buster
  • Bacon sides
  • Pole exe
  • Pale Box
  • Stamped envelope (.04)
  • Bacon sides
  • 2 doz henning??
  • Paid with Reg. 162 old casting

Obviously, Buster is a nickname for someone, but who?  Whoever, he was, he had a vest, hat, coat, pants and shelves.

In addition, Ezekiel Estes submitted a bill to the estate for $21.18 for shingling the house and Susannah’s doctor bill was $51.  She died a slow death of a heart ailment.

Mary Mildred Estes

Above, George Estes’s granddaughter, Susannah’s daughter, Mary Mildred Estes born April 3, 1828 and died Jan. 20, 1917 in Lynchburg, VA., married William Greenwood and second, Jesse Jacobs..

Susannah’s son, Ezekiel Estes, below, born in 1814 and died in 1885 in Halifax County, married Martha Barley.

Ezekiel Estes

A few years after I published this original article, Jerry, one of Ezekiel’s descendants contacted me and provided this amazing watch case that was owned by Ezekiel.

Ezekiel Estes watch case 2.jpg

Ezekiel Estes watch case

This beautiful pocket-watch case is made of Iroquoian beadwork, causing me to wonder how he obtained it, and if there is more significance than a simple watch case. Regardless, it’s beautiful and a huge thank you to Jerry for sharing so that everyone can enjoy this treasure.

The Court

George Estes himself had a few encounters with the legal system. People at that time seemed to be quite litigious, and George was involved with no fewer than 14 nonfamily cases, generally as a defendant, and went to court even more often as a witness.

Court days, which initially happened quarterly, then monthly, were quite the social event in the 1700s and 1800s in Virginia. Anyone who was anyone attended, and much business was transacted outside the courthouse and in the taverns. It was also one of the best ways to hear the news as well as see the news being made. The original reality TV!

I recall that when my daughter and I first went to Halifax County, we visited the clerk’s office asking asked about the various record books and such. My daughter had the book of court notes out, and was looking in the plaintiff’s index. We told the lady that we were looking for Estes and she said “Oh, well then, your people are in this book”, and retrieved the defendants ledger. Things haven’t changed much over the years apparently. The Estes family is legendary, or at least infamous!

George’s first court appearance was in 1786 when he was prosecuted for “profane swearing.” In one case, George and his father Moses were involved as witnesses in a lawsuit where someone signed a document they later regretted after partaking of the fruit brandy at the Estes home. The Estes family was well known for its fine orchards. The fruit brandies were kept cool in a special compartment under the foundation of the house.

In 1802, George put a mortgage on his household items which included 2 feather beds and furniture for 9 pounds, 2 shillings and 2 pence. You can tell that of their household goods, the coveted items were the feather beds.

In 1837, George gave a deposition in the chancery case of Light vs Yuonger wherein the descendants of Thomas Younger battled, for years, over the estate of Thomas Younger after his daughter Rachel Younger died. Thomas was likely the great-uncle of George’s wife, Mary Younger. While the outcome of the case actually doesn’t involve our family directly, we do find a deposition given by George in 1837 over the value of a slave named Peter who in 1812 had been disabled with elephantitis. George, age 74 at that time, signed his deposition.

George Estes 1837 deposition in Light vs Younger.jpg

Moses’s Land

Most of the court cases, not included in the 15 non-family cases mentioned above, involved years and years of appearances having to do with Moses estate settlement which was finally settled in 1837, 24 years after Moses’s death. George, the eldest son, was 74 years old when his father’s estate was settled and he immediately deeded his portion of the land to Susannah.

This family battled over land and inheritance for generations, beginning in 1813 with Moses’ death, followed by George’s children and then Susannah’s and continuing into the present generations whose parents were still involved with that land until the county took the land by eminent domain. At least one person refused to sell the land and instead has a ‘long-term lease”, although what they think they’ll do with a stinky landfill is beyond me. I suspect it was a matter of principle.

When I visited Halifax County, two elderly living cousins, Doug and Shirley, both now deceased, remembered the land from their childhood. Shirley told me that the original home burned in about 1933, complete with all of the family photos, Bibles, etc. She remembers that someone on the school bus told her that her grandparents’ house burned the night before.

Shirley explained that the original home had 8 rooms with 2 fireplaces. Photos of “all the family” hung there – but of course burned in the fire which is why none exist today, according to Shirley. The home had a cellar – which is reflected in a chancery suit where it’s mentioned that Moses Estes stored his fruit brandy in the cellar. Apparently, one night, someone drank too much and signed something they regretted at Moses’s house.  Although Moses wasn’t the signer, Shirley mentioned that the Estes men liked their alcohol a bit too much and alcoholism ran in the family.

Shirley said there were blackberries, raspberries, huge mulberry trees “three foot through,” grapes and 5 cherry trees. I suspect there were apple and peach trees too back when Moses planted his orchard.

Shirley said that there were three springs, making this property quite desirable for homesteading. The Walker spring is about half a mile away, the Waddell spring, and a small spring by the house which had a spring-house built over it to cool things like milk. They grew gourds, and there were always “dipping gourds” by the springs so anyone could get a cool drink of water.

The Estes family used to haul ice, cut from the river, hoping it would last until at least partway through the summer. One time Ezekiel Estes, Moses’s great-grandson through granddaughter Susannah was hauling ice for one Mr. Willingham using steers instead of oxen. When asked if he couldn’t make those steers go faster, Zeke said “no, cause I need them tomorrow.”

The man who bulldozed the property after the city purchased it told me there were 3 houses “back there,” all “farm type” homes. Apparently the first home built was a log cabin, probably about 1782 when the family first arrived from Amelia County, and it was later used for the young couples after they were first married.

The home that burned was described as a large 2 story home with upper and lower porches all around. Porches are important in the south.

There has been a great deal of speculation about why George provided only for his daughter Susannah. It could be because she was not married and he felt protective towards her, wanting to provide for her and his grandchildren after his passing. She was very young, 13 or 14 when she became pregnant, and it would be easy to see how he could have been especially protective of her and her children whom he had lived with for their entire lives. In essence, George raised her children as his own, especially Ezekiel who was the eldest. Ezekiel was born right about the time that George and Mary stopped having children, so Ezekiel probably just fit perfectly into the stair-steps of children.

It could also be that George gave his worldly good to Susannah because she took care of George in his old age – although that wouldn’t explain the 1830s deeds. George’s wife Mary probably died sometime between 1820 and 1830, and certainly before George started deeding to Susannah in 1833, because Mary signed no release  of dower rights.

Others have suggested that perhaps Susannah might have been an opportunist and perhaps manipulative or devious. Some have questioned the propriety of the situation. Susannah had only two male children. Her oldest, Ezekiel, has descendants who have DNA tested and they match a Moore family that lived in the area, although not the same Moore family that Susannah’s brother, John R. Estes married into.

I think it suffices to say that George, Susannah and Ezekiel were extremely close and given the social stigma attached to illegitimate birth in that era, let alone 5 illegitimate children, the family was probably increasingly subject to harsh scrutiny, discrimination, criticism and were socially marginalized. One hint may be held in George’s 1833 Revolutionary War pension application where he states there is no clergy in his neighborhood, but the oldest church in the county is but a few blocks down the street from his home, within walking distance. One can certainly understand why and how George could and would feel a great deal of affection for his grandchildren in particular, as he apparently lived with them as they grew up. There are several records that involve both George and Ezekiel who probably looked up to his grandfather as a role model.

In fact, it was Ezekiel Estes who reported the death of George Estes and said that he was 100 years and 4 months old, born in Amelia County. I hope, for George’s sake, that the family had a bang up 100 year old birthday celebration where everyone came to visit and eat that fine southern food, even if we know today they were a few years early. Or maybe George really was 100 years old in 1859 and simply misstated his birth year in 1833. Regardless, I hope they had a wonderful celebration and he had many guests who sat and visited and imbibed some of that fine Estes brandy! I wish I could hear the stories of his hundred years of life.  What a gift that would be.

Estes Cem white stones

George is reportedly buried here in the Estes section of the Oak Ridge Cemetery immediately to the right just inside the entrance.  The Estes family markers are all bright white here after being cleaned by now deceased cousin Nancy Osborne.  We don’t know exactly where Susannah, George with his wife Mary Younger and Moses with his wife Luremia Combs are buried, but rest assured that they are here among their descendants and family members.

It’s believed that George and Mary are buried in the unmarked area, below.

Estes cem vacant stop

In the following photograph, the picture is taken from behind the stones, before they were cleaned and restored, with the original Estes land showing across the street.  The Estes homestead was behind these houses which stand on part of Moses’ land that was sold off by descendants.  The original homestead is now the landfill, although some forest was preserved as a barrier between these homes and the landfill the last time I in visited in 2006 or so.  The cobblestones showing in the wall below are the original road cobblestones that George probably helped to lay.

Estes cem and wall

I would like to have a Revolutionary War marker placed for George Estes in the cemetery so that he will be honored and his grave will be marked for future generations.

George certainly lived an amazing life.  He was born in Amelia County during the French and Indian war, as his father and uncles serving in that conflict.  About 1770, the Moses Estes family migrated in mass, it seems, to Halifax County where his father and grandfather, both named Moses, established homes, albeit a few miles apart.

About the time George came of age, he volunteered to take his father’s place in the Revolutionary War.  After returning home, just a month later, his own “slot” came up, so he then served for himself.

Many Estes men were pushing the new frontier.  In fact, George moved an Estes family to Hawkins County, TN, probably offering to help in order to see a bit of the world.  He stayed for almost a year, and it was from there in October of 1782 that he enlisted as a volunteer to serve his third stint in the military in the Revolutionary War.  George obviously saw a lot and probably talked about that part of the country to his children when telling tales about his great adventure.  He’s one of the very few men I’ve ever heard of going BACK home from the frontier, and staying there.  His son, John R. Estes would eventually settle in Claiborne County, TN himself, some 30+ years later, near where his father was in what would become Eastern Tennessee.

We don’t know much about George’s religious leanings.  When he was young and first married, church attendance was required in the Anglican church.  That’s also about the time he was prosecuted for “profane swearing.”

We know that his wife, Mary Younger’s family was probably Methodist, a dissenting religion, but one that was “legal” by the 1780s.  Given that his son, John R. Estes married the minister’s daughter, in all likelihood, this family was Methodist.  Whether George was enthusiastically Methodist too, “went along” begrudgingly and slept through services in the back row or simply stayed at home, we’ll never know.  We do know, per a deposition, that George Estes was with the Reverend William Moore’s family on Christmas Day, 1811.  George’s son, John R. Estes was married to Reverend William Moore’s daughter, Ann Moore.

At least two of George’s children ran badly afoul of either the law of the social norms of the time.  Son William drank to excess and daughter Susannah had five children out of wedlock, as a pattern occurrence.  This would have made it difficult for the rest of George’s children to “marry well” because something like that paints the entire family with the same brush.

Today, it’s inconceivable to us, but at that time, people who were born “out-of-wedlock” really could only marry others of their same social status.  Interracial marriages were outlawed and the choices people had, both legally and in reality were much more limited than today.  Remember, I told you that the county clerk still knew that the Estes’s would be found in the “defendants” book???  Maybe this is part of why so many descendants left for lands where there was less judgment waiting and one could start anew, without stigma already attached from the behavior of others.

George’s wife Mary would pass away sometime between about 1820 and 1830.  George would have been between 60 and 70 years old at that time, and would live almost another 30-40 years.

After Mary’s death, it appears that Susannah took care of George.  Given that by this time, Susannah had 5 illegitimate children she had to provide for, George’s pension probably took care of Susannah as well.  I wonder how military pensions were figured at that time.  I would have thought they would all have been relatively equal for the same rank (private), and if unequal, perhaps George received something for each of his three stints in the military.  By way of contrast, his son, John R. Estes who served in the War of 1812 was collecting a pension at the same time received $8 a month as compared to George’s $31 year, which breaks down to $2.58 per month.  In the end, Susannah wound up with all of George’s assets although, clearly, his pension stopped when he died.

By the time George died, his son Marcus had passed away, possibly in the War of 1812, and there are a couple of children I lose in the records, but as far as we know, most of George’s children outlived him. Some had moved west but George still had Polly, Susannah and William Y. nearby, although William Y. seemed unable to even help himself, due to his drinking, based on numerous court records.

The good news is that because of where Moses’s land was located, and the ability to locate the Oak Ridge Cemetery today, then track through the landfill deeds and family records, we were able to find the original Estes land.

Furthermore, we know that graves were moved from the Estes cemetery, now under the landfill, to the Estes plot in the Oak Ridge Cemetery, which may have been the original Estes cemetery in the first place.

All I know is that when cousin Nancy started talking about having moved the graves and finding the collar bone of Moses Estes, I just couldn’t stop myself from thinking about DNA.  I know fully well that today, even with enough money, that the retrieval of ancient DNA for consumer purposes really isn’t a viable option.  But I also know that in another decade, with the advances in technology and the associated drop in prices, combined with what has been able to be accomplished with sequencing ancient genomes – that eventually – that collarbone would have been useful.

I know, bad genealogist, bad genealogist.  Bad, bad, bad.  I can’t help it.  It’s that nonconformant Estes side coming out!  It’s in my genes.  I can’t help it.  In fact, I know where there’s a bone we can dig up to prove it….

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A Study Utilizing Small Segment Matching

There has been quite a bit of discussion in the last several weeks, both pro and con, about how to use small matching DNA segments in genetic genealogy.  A couple of people are even of the opinion that small segments can’t be used at all, ever.  Others are less certain and many of us are working our way through various scenarios.  Evidence certainly exists that these segments can be utilized.

I’ve been writing foundation articles, in preparation for this article, for several weeks now.  Recently, I wrote about how phasing works and determining IBD versus IBS matches and included guidelines for telling the difference between the different kinds of matches.  If you haven’t read that article, it’s essential to understanding this article, so now would be a good time to read or review that article.

I followed that with a step by step article, Demystifying Autosomal DNA Matching, on how to do phasing and matching in combination with the guidelines about how to determine IBD (identical by descent) versus IBS (identical by chance) and identical by population matches when evaluating your own matches.

Now that we understand IBS, IBD, Phasing and how matching actually works on a case by case basis, let’s look at applying those same matching and IBS vs IBD guidelines to small data segments as well.

A Little History

So those of you who haven’t been following the discussion on various blogs and social media don’t feel like you’ve been dropped into the middle of a conversation with no context, let me catch you up.

On Thanksgiving Day, I published an article about identifying one of my ancestors, after many years of trying, Sarah Hickerson.

That article spurred debate, which is just fine when the debate is about the science, but it subsequently devolved into something less pleasant.  There are some individuals with very strong opinions that utilizing small segments of DNA data can “never be done.”

I do not agree with that position.  In fact, I strongly disagree and there are multiple cases with evidence to support small segments being both accurate and useful in specific types of genealogical situations.  We’ll take a look at several.

I do agree that looking at small segment data out of context is useless.  To the best of my knowledge, no genealogist begins with their smallest segments and tries to assemble them, working from the bottom up.  We all begin with the largest segments, because they are the most useful and the closest connections in our tree, and work our way down.  Generally, we only work with small segments when we have to – and there are times that’s all we have.  So we need to establish guidelines and ways to know if those small segments are reliable or not.  In other words, how can we draw conclusions and how much confidence can we put in those conclusions?

Ultimately, whether you choose to use or work with small segment data will be your own decision, based on your own circumstances.  I simply wanted to understand what is possible and what is reasonable, both for my own genealogy and for my readers.

In my projects, I haven’t been using small segment data out of context, or randomly.  In other words, I don’t just pick any two small segment matches and infer or decide that they are valid matches.  Fortunately, by utilizing the IBD vs IBS guidelines, we have tools to differentiate IBD (Identical by Descent) segments from IBS (Identical by State) by chance segments and IBD/IBS by population for matching segments, both large and small.

Studying small segment data is the key to determining exactly how small segments can reasonably be utilized.  This topic probably isn’t black or white, but shades of gray – and assuming the position that something can’t be done simply assures that it won’t be.

I would strongly encourage those involved and interested in this type of research to retain those small segments, work with them and begin to look for patterns.  The only way we, as a community, are ever going to figure out how to work with small segments successfully and reliably is to, well, work with them.

Discussing the science and scenarios surrounding the usage of small data segments in various different situations is critical to seeing our way through the forest.  If the answers were cast in concrete about how to do this, we wouldn’t be working through this publicly today.

Negative personal comments and inferences have no place in the scientific community.  It discourages others from participating, and serves to stifle research and cooperation, not encourage it.  I hope that civil scientific discussions and comparisons involving small segment data can move forward, with decorum, because they are critically needed in order to enhance our understanding, under varying circumstances, of how to utilize small segment data.  As Judy Russell said, disagreeing doesn’t have to be disagreeable.

Two bloggers, Blaine Bettinger and CeCe Moore wrote articles following my Hickerson article.  Blaine subsequently wrote a second article here.  Felix Immanuel wrote articles here and here.

A few others have weighed in, in writing, as well although most commentary has been on Facebook.  Israel Pickholtz, a professional genealogist and genetic consultant, stated on his blog, All My Foreparents, the following:

It is my nature to distrust rules that put everything into a single category and that’s how I feel about small segments. Sometimes they are meaningful and useful, sometimes not.

When I reconstructed my father’s DNA using Lazerus (described last week in Genes From My Father), I happily accepted all small segments of whatever size because those small segments were in the DNA of at least one of his children and at least one of his brother/sister/first cousin. If I have a particular small segment, I must have received it from my parents. If my father’s brother (or sister) has it as well, then it is eminently clear to me that I got it from my father and that it came to him and his brother from my grandfather. And it is not reasonable to say that a sliver of that small segment might have come from my mother, because my father’s people share it.

After seeing Israel’s commentary about Lazarus, I reconstructed the genome of both Roscoe and John Ferverda, brothers, which includes both large and small segments.  Working with the Ferverda DNA further, I wrote an article, Just One Cousin, about matching between two siblings and a first cousin, which includes lots of small data segments, some of which were proven to triangulate, meaning they are genuine, and some which did not.  There are lots more examples in the demystifying article, as well.

What Not To Do 

Before we begin, I want to make it very clear that am not now, and never have, advocated that people utilize small data segments out of context of larger matching segments and/or at least suspected matching genealogy.  For example, I have never implied or even hinted that anyone should go to GedMatch, do a “one to many” compare at 1 cM and then contact people informing them that they are related.  Anyone who has extrapolated what I’ve written to mean that either simply did not understand or intentionally misinterpreted the articles.

Sarah Hickerson Revisited

If I thought Sarah Hickerson caused me a lot of heartburn in the decades before I found her, little did I know how much heartburn that discovery would cause.

Let’s go back to the Sarah Hickerson article that started the uproar over whether small data segments are useful at all.

In that article, I found I was a member of a new Ancestry DNA Circle for Charles Hickerson and Mary Lytle, the parents of Sarah Hickerson.

Ancestry Hickerson match

Because there are no tools at Ancestry to prove DNA connections, I hurried over to Family Tree DNA looking for any matches to Hickersons for myself and for my Vannoy cousins who also (potentially) descended from this couple.  Much to my delight, I found  several matches to Hickersons, in fact, more than 20 – a total of 614 rows of spreadsheet matches when I included all of my Vannoy cousins who potentially descend from this couple to their Hickerson matches.  There were 64 matching clusters of segments, both small and large.  Some matches were as large as 20cM with 6000 SNPs and more than 20 were over 10cM with from 1500 to 6000 SNPs.  There were also hundreds of small segments that matched (and triangulated) as well.

By the time I added in a few more Vannoy cousins that we’ve since recruited, the spreadsheet is now up to 1093 rows and we have 52 Vannoy-Hickerson TRIANGULATED CLUSTERS utilizing only Family Tree DNA tools.

Triangulated DNA, found in 3 or more people at the same location who share a common ancestor is proven to be from that ancestor (or ancestral couple.)  This is the commonly accepted gold standard of autosomal DNA triangulation within the industry.

Here’s just one example of a cluster of three people.  Charlene and Buster are known (proven, triangulated) cousins and Barbara is a descendant of Charles Hickerson and Mary Lytle.

example triang

What more could you want?

Yes, I called this a match.  As far as I’m concerned, it’s a confirmed ancestor.  How much more confirmed can you get?

Some clusters have as many as 25 confirmed triangulated members.

chr 13 group

Others took issue with this conclusion because it included small segment data.  This seems like the perfect opportunity in which to take a look at how small segments do, or don’t stand up to scrutiny.  So, let’s do just that.  I also did the same type of matching comparison in a situation with 2 siblings and a known cousin, here.

To Trash…or Not To Trash

Some genetic genealogists discard small segments entirely, generally under either 5 or 7cM, which I find unfortunate for several reasons.

  1. If a person doesn’t work with small segments, they really can’t comment on the lack of results, and they’ll never have a success because the small segments will have been discarded.
  2. If a person doesn’t work with small segments, they will never notice any trends or matches that may have implications for their ancestry.
  3. If a person doesn’t work with small segments, they can’t contribute to the body of evidence for how to reasonably utilize these segments.
  4. If a person doesn’t work with small segments, they may well be throwing the baby out with the bathwater, but they’ll never know.
  5. They encourage others to do the same.

The Sarah Hickerson article was not meant as a proof article for anything – it was meant to be an article encouraging people to utilize genetic genealogy for not only finding their ancestor and proving known connections, but breaking down brick walls.  It was pointing the way to how I found Sarah Hickerson.  It was one of my 52 Ancestors Series, documenting my ancestors, not one of the specifically educational articles.  This article is different.

If you are only interested in the low hanging fruit, meaning within the past 5 or 6 generations, and only proving your known pedigree, not finding new ancestors beyond that 5-6 generation level, then you can just stop reading now – and you can throw away your small segments.  But if you want more, then keep reading, because we as a community need to work with small segment data in order to establish guidelines that work relative to utilizing small segments and identifying the small segments that can be useful, versus the ones that aren’t.

I do not believe for one minute that small segments are universally useless.  As Israel said, if his family did not receive those segments from a common family member, then where did they all get those matching segments?

In fact, utilizing triangulated and proven DNA relationships within families is how adoptees piece together their family trees, piggybacking off of the work of people with known pedigrees that they match genetically.  My assumption had been that the adoptee community utilized only large DNA segments, because the larger the matching segments, generally the closer in time the genealogy match – and theoretically the easier to find.

However, I discovered that I was wrong, and the adoptee community does in fact utilize small segments as well.  Here’s one of the comments posted on my Chromosome Browser War blog article.

“Thanks for the well thought out article, Roberta, I have something to add from the folks at DNAadoption. Adoptees are not just interested in the large segments, the small segments also build the proof of the numerous lines involved. In addition, the accumulation of surnames from all the matches provides a way to evaluate new lines that join into the tree.”

Diane Harman-Hoog (on behalf of the 6 million adoptees in this country, many of who are looking for information on medical records and family heritage).

Diane isn’t the only person who is working with small segment data.  Tim Janzen works with small segments, in particular on his Mennonite project, and discusses small segments on the ISOGG WIKI Phasing page.  Here is what Tim has to say:

“One advantage of Family Finder is that FF has a 1 cM threshold for matching segments. If a parent and a child both have a matching segment that is in the 2 to 5 cM range and if the number of matching SNPs is 500 or more then there is a reasonably high likelihood that the matching segment is IBD (identical by descent) and not IBS (identical by state).”

The same rules for utilizing larger segment data need to be applied to small segment data to begin with.

Are more guidelines needed for small segments?  I don’t know, but we’ll never know if we don’t work with many individual situations and find the common methods for success and identify any problematic areas.

Why Do Small Segments Matter?

In some cases, especially as we work beyond the 6 generation level, small segments may be all we have left of a specific ancestor.  If we don’t learn to recognize and utilize the small segments available to us, those ancestors, genetically speaking, will be lost to us forever.

As we move back in time, the DNA from more distant ancestors will be divided into smaller and smaller segments, so if we ever want the ability to identify and track those segments back in time to a specific ancestor, we have to learn how to utilize small segment data – and if we have deleted that data, then we can’t use it.

In my case, I have identified all of my 5th generation ancestors except one, and I have a strong lead on her.  In my 6th generation, however, I have lots of walls that need to be broken through – and DNA may be the only way I’ll ever do that.

Let’s take a look at what I can expect when trying to match people who also descend from an ancestor 5 generations back in time.  If they are my same generation, they would be my fourth cousins.

Based on the autosomal statistics chart at ISOGG, 4th cousins, on the average, would expect to share about 13.28 cM of DNA from their common ancestor.  This would not be over the match threshold at FTDNA of approximately 20 cM total, and if those segments were broken into three pieces, for example, that cousin would not show as a match at either FTDNA or 23andMe, based on the vendors’ respective thresholds.

% Shared DNA Expected Shared cM Relationship
0.781% 53.13 Third cousins, common ancestor is 4 generations back in time
0.391% 26.56 Third cousins once removed
20 cm Family Tree DNA total cM Threshold
0.195% 13.28 Fourth cousins, common ancestor is 5 generations back in time
7 cM 23andMe individual segment cM match threshold
0.0977% 6.64 Fourth cousins once removed
0.0488% 3.32 Fifth cousins, common ancestor is 6 generations back in time
0.0244 1.66 Fifth cousins once removed

If you’re lucky, as I was with Hickerson, you’ll match at least some relative who carries that ancestral DNA line above the threshold, and then they’ll match other cousins above the threshold, and you can build a comparison network, linking people together, in that fashion.  And yes you may well have to utilize GedMatch for people testing at various different vendors and for those smaller segment comparisons.

For clarification, I have never “called” a genealogy match without supporting large segment data.  At the vendors, you can’t even see matches if they don’t have larger segments – so there is no way to even know you would match below the threshold.

I do think that we may be able to make calls based on small segments, at least in some instances, in the future.  In fact, we have to figure out how to do this or we will rarely be able to move past the 5th or 6th generation utilizing genetics.

At the 5th generation, or third cousins, one expects to see approximately 26 cM of matching DNA, still over the threshold (if divided correctly), but from that point further back in time, the expected shared amount of DNA is under the current day threshold.  For those who wonder why the vendors state that autosomal matches are reliable to about the 5th or 6th generation, this is the answer.

I do not discount small segments without cause.  In other words, I don’t discount small segments unless there is a reason.  Unless they are positively IBS by chance, meaning false, and I can prove it, I don’t disregard them.  I do label them and make appropriate notes.  You can’t learn from what’s not there.

Let me give you an example.  I have one area of my spreadsheet where I have a whole lot of segments, large and small, labeled Acadian.  Why?  Because the Acadians are so intermarried that I can’t begin to sort out the actual ancestor that DNA came from, at least not yet…so today, I just label them “Acadian.”

This example row is from my master spreadsheet.  I have my Mom’s results in my spreadsheet, so I can see easily if someone matches me and Mom both. My rows are pink.  The match is on Mom’s side, which I’ve color coded purple.  I don’t know which ancestor is the most recent common ancestor, but based on the surnames involved, I know they are Acadian.  In some cases, on Acadian matches, I can tell the MRCA and if so, that field is completed as well.

Me Mom acadian

As a note of interest, I inherited my mother’s segment intact, so there was no 50% division in this generation.

I also have segments labeled Mennonite and Brethren.  Perhaps in the future I’ll sort through these matches and actually be able to assign DNA segments to specific ancestors.  Those segments aren’t useless, they just aren’t yet fully analyzed.  As more people test, hopefully, patterns will emerge in many of these DNA groupings, both small and large.

In fact, I talked about DNA patterns and endogamous populations in my recent article, Just One Cousin.

For me, today, some small segment matches appear to be central European matches.  I say “appear to be,” because they are not triangulated.  For me this is rather boring and nondescript – but if this were my African American client who is trying to figure out which line her European ancestry came from, this could be very important.  Maybe she can map these segments to at least a specific ancestral line, which she would find very exciting.

Learning to use small segments effectively has the potential to benefit the following groups of people:

  • People with colonial ancestry, because all that may be left today of colonial ancestors is small segments.
  • People looking to break down brick walls, not just confirm currently known ancestors.
  • People looking for minority ancestors more than 5 or 6 generations back in their trees.
  • Adoptees – although very clearly, they want to work with the largest matches first.
  • People working with ethnic identification of ancestors, because you will eventually be able to track ethnicity identifying segments back in time to the originating ancestor(s).

Conversely, people from highly endogamous groups may not be helped much, if at all, by small segments because they are so likely to be widely shared within that population as a group from a common ancestor much further back in time.  In fact, the definition of a “small segment” for people with fully endogamous families might be much larger than for someone with no known endogamy.

However, if we can identify segments to specific populations, that may help the future accuracy of ethnicity testing.

Let’s go back and take a look at the Hickerson data using the same format we have been using for the comparisons so far.

Small Segment Examples

These Hickerson/Vannoy examples do not utilize random small segment matches, but are utilizing the same matching rules used for larger matches in conjunction with known, triangulated cousin groups from a known ancestor.  Many cousins, including 2 brothers and their uncle all carry this same DNA.  Like in Israel’s case, where did they get that same DNA if not from a common ancestor?

In the following examples, I want to stress that all of the people involved DO HAVE LARGER SEGMENT MATCHES on other chromosomes, which is how we knew they matched in the first place, so we aren’t trying to prove they are a match.  We know they are.  Our goal is to determine if small segments are useful in the same situation, proving matches, as with larger segments.  In other words, do the rules hold true?  And how do we work with the data?  Could we utilize these small segment matches if we didn’t have larger matching segments, and if so, how reliable would they be?

There is a difference between a single match and a triangulated group:

  • Matches between two people are suggestive of a common ancestor but could be IBS by chance or population..
  • Multiple matches, such as with the 6 different Hickersons who descend from Charles Hickerson and Mary Lytle, both in the Ancestry DNA Circle and at Family Tree DNA, are extremely suggestive of a specific common ancestor.
  • Only triangulated groups are proof of a common ancestor, unless the people are  closely related known relatives.

In our Hickerson/Vannoy study, all participants match at least to one other (but not to all other) group members at Family Tree DNA which means they match over the FTDNA threshold of approximately 20 cM total and at least one segment over 7.7cM and 500 SNPs or more.

In the example below, from the Hickerson article, the known Vannoy cousins are on the left side and the Hickerson matches to the Vannoy cousins are across the top.  We have several more now, but this gives you an idea of how the matching stacked up initially.  The two green individuals were proven descendants from Charles Hickerson and Mary Lytle.

vannoy hickerson higginson matrix

The goal here is to see how small data segments stack up in a situation where the relationship is distant.  Can small segments be utilized to prove triangulation?  This is slightly different than in the Just One Cousin article, where the relationship between the individuals was close and previously known.  We can contrast the results of that close relationship and small segments with this more distant connection and small segments.

Sarah Hickerson and Daniel Vannoy

The Vannoy project has a group of about a dozen cousins who descend from Elijah Vannoy who have worked together to discover the identify of Elijah’s parents.  Elijah’s father is one of 4 Vannoy men, all sons of the same man, found in Wilkes County, NC. in the late 1700s.  Elijah Vannoy is 5 generations upstream from me.

What kind of evidence do we have?  In the paper genealogy world, I have ruled out one candidate via a Bible record, and probably a second via census and tax records, but we have little information about the third and fourth candidates – in spite of thoroughly perusing all existent records.  So, if we’re ever going to solve the mystery, short of that much-wished-for Vannoy Bible showing up on e-Bay, it’s going to have to be via genetic genealogy.

In addition to the dozen or so Vannoy cousins who have DNA tested, we found 6 individuals who descend from Sarah Hickerson’s parents, Charles Hickerson and Mary Lytle who match various Vannoy cousins.  Additionally, those cousins match another 21 individuals who carry the Hickerson or derivative surnames, but since we have not proven their Hickerson lineage on paper, I have not utilized any of those additional matches in this analysis.  Of those 26 total matches, at Family Tree DNA, one Hickerson individual matches 3 Vannoy cousins, nine Hickerson descendants match 2 Vannoy cousins and sixteen Hickerson descendants match 1 Vannoy cousin.

Our group of Vannoy cousins matching to the 6 Charles Hickerson/Mary Lytle descendants contains over 60 different clusters of matching DNA data across the 22 chromosomes.  Those 6 individuals are included in 43 different triangulated groups, proving the entire triangulation group shares a common ancestor.  And that is BEFORE we add any GedMatch information.

If that sounds like a lot, it’s not.  Another recent article found 31 clusters among siblings and their first cousin, so 60 clusters among a dozen known Vannoy cousins and half a dozen potential Hickerson cousins isn’t unusual at all.

To be very clear, Sarah Hickerson and Daniel Vannoy were not “declared” to be the parents of Elijah Vannoy, born in 1784, based on small segment matches alone.  Larger segment matches were involved, which is how we saw the matches in the first place.  Furthermore, the matches triangulated.  However, small segments certainly are involved and are more prevalent, of course, than large segments.  Some cousins are only connected by small segments.  Are they valid, and how do we tell?  Sometimes it’s all we have.

Let me give you the classic example of when small segments are needed.

We have four people.  Person A and B are known Vannoy cousins and person C and D are potential Hickerson cousins.  Potential means, in this case, potential cousins to the Vannoys.  The Hickersons already know they both descend from Charles Hickerson and Mary Lytle.

  • Person A matches person C on chromosome 1 over the matching threshold.
  • Person B matches person D on chromosome 2 over the matching threshold.

Both Vannoy cousins match Hickerson cousins, but not the same cousin and not on the same segments at the vendor.  If these were same segment matches, there would be no question because they would be triangulated, but they aren’t.

So, what do we do?  We don’t have access to see if person C and D match each other, and even if we did, they don’t match on the same segments where they match persons A and B, because if they did we’d see them as a match too when we view A and B.

If person A and B don’t match each other at the vendor, we’re flat out of luck and have to move this entire operation to GedMatch, assuming all 4 people have or are willing to download their data.

a and b nomatch

If person A and B match each other at the vendor, we can see their small segment data as compared to each other and to persons C and D, respectively which then gives us the ability to see if A matches C on the same small segment as B matches D.

a and b match

If we are lucky, they will all show a common match on a small segment – meaning that A will match B on a small segment of chromosome 3, for example, and A will match C on that same segment.  In a perfect world, B will also match D on that same segment, and you will have 4 way triangulation – but I’m happy with the required 3 way match to triangulate.

This is exactly what happened in the article, Be Still My H(e)art.  As you can see, three people match on chromosomes 1 and 8, below – two of whom are proven cousins and the third was the wife surname candidate line.

Younger Hart 1-8

The example I showed of chromosome 2 in the Hickerson article was where all participants of the 5 individuals shown on the chromosome browser were matching to the Vannoy participant.  I thought it was a good visual example.  It was just one example of the 60+ clusters of cousin matches between the dozen Vannoy cousins and 6 Hickerson descendants.

This example was criticized by some because it was a small segment match.  I should probably have utilized chromosome 15 or searched for a better long segment example, but the point in my article was only to show how people that match stack up together on the chromosome browser – nothing more.   Here’s the entire chromosome, for clarity.

hickerson vannoy chr 2

Certainly, I don’t want to mislead anyone, including myself.  Furthermore, I dislike being publicly characterized as “wrong” and worse yet, labeled “irresponsible,” so I decided to delve into the depths of the data and work through several different examples to see if small segment data matching holds in various situations.  Let’s see what we found.

Chromosome 15

I selected chromosome 15 to work with because it is a region where a lot of Vannoy descendants match – and because it is a relatively large segment.  If the Hickersons do match the Vannoys, there’s a fairly good change they might match on at least part of that segment.  In other words, it appears to be my best bet due to sheer size and the number of Elijah Vannoy’s descendants who carry this segment.  In addition to the 6 individuals above who matched on chromosome 15, here are an additional 4.  As you can see, chromosome 15 has a lot of potential.

Chrom 15 Vannoy

The spreadsheet below shows the sections of chromosome 15 where cousins match.  Green individuals in the Match column are descendants of Charles Hickerson and Mary Lytle, the parents of Sarah Hickerson.  The balance are Vannoys who match on chromosome 15.

chr 15 matches ftdna v4

As you can see, there are several segments that are quite large, shown in yellow, but there are also many that are under the threshold of 7cM, which are all  segments that would be deleted if you are deleting small segments.  Please also note that if you were deleting small segments, all of the Hickerson matches would be gone from chromosome 15.

Those of you with an eagle eye will already notice that we have two separate segments that have triangulated between the Vannoy cousins and the Hickerson descendants, noted in the left column by yellow and beige.  So really, we could stop right here, because we’ve proven the relationship, but there’s a lot more to learn, so let’s go on.

You Can’t Use What You Can’t See

I need to point something out at this point that is extremely important.

The only reason we see any segment data below the match threshold is because once you match someone on a larger segment at Family Tree DNA, over the threshold, you also get to view the small segment data down to 1cM for your match with that person. 

What this means is that if one person or two people match a Hickerson descendant, for example you will see the small segment data for their individual matches, but not for anyone that doesn’t match the participant over the matching threshold.

What that means in the spreadsheet above, is that the only Hickerson that matches more than one Vannoy (on this segment) is Barbara – so we can see her segment data (down to 1cM ) as compared to Polly and Buster, but not to anyone else.

If we could see the smaller segment data of the other participants as compared to the Hickerson participants, even though they don’t match on a larger segment over the matching threshold, there could potentially be a lot of small segment data that would match – and therefore triangulate on this segment.

This is the perfect example of why I’ve suggested to Family Tree DNA that within projects or in individuals situations, that we be allowed to reduce the match threshold – especially when a specific family line match is suspected.

This is also one of the reasons why people turn to GedMatch, and we’ll do that as well.

What this means, relative to the spreadsheet is that it is, unfortunately, woefully incomplete – and it’s not apples to apples because in some cases we have data under the match threshold, and in some, we don’t.  So, matches DO count, but nonmatches where small segment data is not available do NOT count as a non-match, or as disproof.  It’s only negative proof IF you have the data AND it doesn’t match.

The Vannoys match and triangulate on many segments, so those are irrelevant to this discussion other than when they match to Hickerson DNA.  William (H), descends from two sons of Charles Hickerson and Mary Lytle.  Unfortunately, he only matches one Vannoy, so we can only see his small segments for that one Vannoy individual, William (V).  We don’t know what we are missing as compared to the rest of the Vannoy cousins.

To see William (H)’s and William (V)’s DNA as compared to the rest of the Vannoy cousins, we had to move to GedMatch.

Matching Options

Since we are working with segments that are proven to be Vannoy, and we are trying to prove/disprove if Daniel Vannoy and Sarah Hickerson are the parents of Elijah through multiple Hickerson matches, there are only a few matching options, which are:

  1. The Hickerson individuals will not triangulate with any of the Vannoy DNA, on chromosome 15 or on other chromosomes, meaning that Sarah Hickerson is probably not the mother of Elijah Vannoy, or the common ancestor is too far back in time to discern that match at vendor thresholds.
  2. The Hickerson individuals will not triangulate on this segment, but do triangulate on other segments, meaning that this segment came entirely from the Vannoy side of the family and not the Hickerson side of the family. Therefore, if chromosome 15 does not triangulate, we need to look at other chromosomes.
  3. The Hickerson individuals triangulate with the Vannoy individuals, confirming that Sarah Hickerson is the mother of Elijah Vannoy, or that there is a different common unknown ancestor someplace upstream of several Hickersons and Vannoys.

All of the Vannoy cousins descend from Elijah Vannoy and Lois McNiel, except one, William (V), who descends from the proven son of Sarah Hickerson and Daniel Vannoy, so he would be expected to match at least some Hickerson descendants.  The 6 Hickerson cousins descend from Charles Hickerson and Mary Lytle, Sarah’s parents.

hickerson vannoy pedigree

William (H), the Hickerson cousin who descends from David, brother to Sarah Hickerson, is descended through two of David Hickerson’s sons.

I decided to utilize the same segment “mapping comparison” technique with a spreadsheet that I utilized in the phasing article, because it’s easy to see and visualize.

I have created a matching spreadsheet and labeled the locations on the spreadsheet from 25-100 based on the beginning of the start location of the cluster of matches and the end location of the cluster.

Each individual being compared on the spreadsheet below has a column across the top.  On the chart below, all Hickerson individuals are to the right and are shown with their cells highlighted yellow in the top row.

Below, the entire colorized chart of chromosome 15 is shown, beginning with location 25 and ending with 100, in the left hand column, the area of the Vannoy overlap.  Remember, you can double click on the graphics to enlarge.  The columns in this spreadsheet are not fully expanded below, but they are in the individual examples.

entire chr 15 match ss v4

I am going to step through this spreadsheet, and point out several aspects.

First, I selected Buster, the individual in the group to begin the comparison, because he was one of the closest to the common ancestor, Elijah Vannoy, genealogically, at 4 generations.  So he is the person at Family Tree DNA that everyone is initially compared against.

Everyone who matches Buster has their matching segments shown in blue.  Buster is shown furthest left.

When participants match someone other than Buster, who they match on that segment is typed into their column.  You can tell who Buster matches because their columns are blue on matching locations.  Here’s an example.

Me Buster match

You can see that in my column, it’s blue on all segments which means I match Buster on this entire region.  In addition, there are names of Carl, Dean, William Gedmatch and Billie Gedmatch typed into the cell in the first row which means at that location, in addition to Buster, I also match Carl and Dean at Family Tree DNA and William (descended from the son of Daniel Vannoy and Sarah Hickerson) at Gedmatch and Billie (a Hickerson) at Gedmatch.  Their name is typed into my column, and mine into theirs.  Please note that I did not run everyone against everyone at GedMatch.  I only needed enough data to prove the point and running many comparisons is a long, arduous process even when GedMatch isn’t experiencing problems.

On cells that aren’t colorized blue, the person doesn’t match Buster, but may still match other Vannoy cousin segments.  For example, Dean, below, matches Buster on location 25-29, along with some other cousins.  However, he does not match Buster on location 30 where he instead matches Harold and Carl who also don’t match Buster at that location. Harold, Carl and Dean do, however, all descend from the same son of Elijah so they may well be sharing DNA from a Vannoy wife at this location, especially since no one who doesn’t share that specific wife’s line matches those three at this location.

Me Buster Dean match

Remember, we are not working with random small data segments, but with a proven matching segment to a common Vannoy ancestor, with a group of descendants from a possible/probable Hickerson ancestor that we are trying to prove/disprove.  In other words, you would expect either a lot of Hickerson matches on the same segments, if Hickerson is indeed a Vannoy ancestral family, or virtually none of them to match, if not.

The next thing I’d like to point out is that these are small segments of people who also have larger matching segments, many of whom do triangulate on larger segments on other chromosomes.  What we are trying to discern is whether small segment matches can be utilized by employing the same matching criteria as large segment matching.  In other words, is small segment data valid and useful if it meets the criteria for an IBD match?

For example, let’s look at Daniel.  Daniel’s segments on chromosome 15, were it not for the fact that he matches on larger segments on other chromosomes, would not be shown as matches, because they are not individually over the match threshold.

Look at Daniel’s column for Polly and Warren.

Daniel matches 2

The segments in red show a triangulated group where Daniel and Warren, or Daniel, Warren and Polly match.  The segments where all 3 match are triangulated.

This proves, unquestionably, that small segments DO match utilizing the normal prescribed IBD matching criteria.  This spreadsheet, just for chromosome 15, is full of these examples.

Is there any reason to think that these triangulated matches are not identical by descent?  If they are not IBD, how do all of these people match the same DNA? Chance alone?  How would that be possible?  Two people, yes, maybe, but 3 or more?  In some cases, 5 or 6 on the same segment?  That is simply not possible, or we have disproven the entire foundation that autosomal DNA matching is based upon.

The question will soon be asked if small segments that triangulate can be useful when there are no larger matching segments to put the match over the initial vendor threshold.

Triangulated Groups

As you can see, most of the people and segments on the spreadsheet, certainly the Elijah descendants, are heavily triangulated, meaning that three or more people match each other on the same locations.  Most of this matching is over the vendor threshold at Family Tree DNA.

You can see that Buster, Me, Dean, Carl and Harold all match each other on the same segments, on the left half of the spreadsheet where our names are in each other’s columns.

triangulated groups

Remember when I said that the spreadsheet was incomplete?  This is an example.  David and Warren don’t match each other at a high enough total of segments to get them over the matching threshold when compared to each other, so we can’t see their small segment data as compared to each other.  David matches Buster, but Warren doesn’t, so I can’t even see them both in relationship to a common match.  There are several people who fall into this category.

Let’s select one individual to use as an example.

I’ve chosen the Vannoy cousin, William(V), because his kit has been uploaded to Gedmatch, he has Vannoy matches and because William is proven to descend from Sarah Hickerson and Daniel Vannoy through their son Joel – so we expect some Hickerson DNA to match William(V).

If William (V) matches the Hickersons on the same DNA locations as he matches to Elijah’s descendants, then that proves that Elijah’s descendant’s DNA in that location is Hickerson DNA.

At GedMatch, I compared William(V) with me and then with Dean using a “one to one” comparison at a low threshold, simply because I wanted as much data as I could get.  Family Tree DNA allows for 1 cM and I did the same, allowing 100 SNPs at GedMatch.  Family Tree DNA’s lowest SNP threshold is 500.

In case you were wondering, even though I did lower the GedMatch threshold below the FTDNA minimum, there were 45 segments that were above 1cM and above 500 SNPs when matching me to William(V), which would have been above the lowest match threshold at FTDNA (assuming we were over the initial match threshold.)  In other words, had we not been below the original match threshold (20cM total, one segment over 7.7cM), these segments would have been included at FTDNA as small segments.  As you can see in the chart below, many triangulated.

I colorized the GedMatch matches, where there were no FTDNA matches, in dark red text.  This illustrates graphically just how much is missed when the small segments are ignored in cases with known or probable cousins.  In the green area, the entry that says “Me GedMatch” could not be colorized red (because you can’t colorize only part of the text of a cell) so I added the Gedmatch designation to differentiate between a match through FTDNA and one from GedMatch.  I did the same with all Gedmatch matches, whether colorized or not.

Let’s take a look and see how small segments from GedMatch affect our Hickerson matching.  Note that in the green area, William (V) matches William (H), the Hickerson descendant, and William (V) matches to me and Dean as well.  This triangulates William (V)’s Hickerson DNA and proves that Elijah’s descendants DNA includes proven Hickerson segments.

William (V) gedmatch matches v2

In this next example, I matched William (H), the Hickerson cousin (with no Vannoy heritage) against both Buster and me.

William (H) gedmatch me buster

Without Gedmatch data, only two segments of chromosome 15 are triangulated between Vannoy and Hickerson cousins, because we can’t see the small data segments of the rest of the cousins who don’t match over the threshold.

You can see here that nearly the entire chromosome is triangulated using small segments.  In the chart below, you can see both William(V) and William (H) as they match various Vannoy cousins.  Both triangulate with me.

William V and William H

I did the same thing with the Hickerson descendant, Billie, as compared to both me and Dean, with the same type of results.

The next question would be if chromosome 15 is a pileup area where I have a lot of IBS matches that are really population based matches.  It does not appear to be.  I have identified an area of my chromosomes that may be a pileup area, but chromosome 15 does not carry any of those characteristics.

So by utilizing the small segments at GedMatch for chromosome 15 that we can’t otherwise see, we can triangulate at least some of the Hickerson matches.  I can’t complete this chart, because several individuals have not uploaded to GedMatch.

Why would the Hickerson descendant match so many of the Vannoy segments on chromosome 15?  Because this is not a random sample.  This is a proven Vannoy segment and we are trying to see which parts of this segment are from a potential Hickerson mother or the Vannoy father.  If from the Hickerson mother, then this level of matching is not unexpected.  In fact, it would be expected.  Since we cheated and saw that chromosome 15 was already triangulated at Family Tree DNA, we already knew what to expect.

In the spreadsheet below, I’ve added the 2 GedMatch comparisons, William (V) to me and Dean, and William (H) to me and Buster.  You can see the segments that triangulate, on the left.  We could also build “triangulated groups,” like GedMatch does.  I started to do this, but then stopped because I realized most cells would be colored and you’d have a hard time seeing the individual triangulated segments.  I shifted to triangulating only the individuals who triangulate directly with the Hickerson descendant, William(H), shown in green.  GedMatch data is shown in red.

chr 15 with gedmatch

I would like to make three points.

1.  This still is not a complete spreadsheet where everyone is compared to everyone.  This was selectively compared for two known Hickerson cousins, William (V) who descends from both Vannoys and Hickersos and William (H) who descends only from Hickersons.

2. There are 25 individually triangulated segments to the Hickerson descendant on just this chromosome to the various Vannoy cousins.  That’s proof times 25 to just one Hickerson cousin.

3.  I would NEVER suggest that you select one set of small segments and base a decision on that alone.  This entire exercise has assembled cumulative evidence.  By the same token, if the rules for segment matching hold up under the worst circumstances, where we have an unknown but suspected relationship and the small segments appear to continue to follow the triangulation rules, they could be expected to remain true in much more favorable circumstances.

Might any of these people have random DNA matches that are truly IBS by chance on chromosome 15?  Of course, but the matching rules, just like for larger segments, eliminates them.  According to triangulation rules, if they are IBS by chance, they won’t triangulate.  If they do triangulate, that would confirm that they received the same DNA from a common ancestor.

If this is not true, and they did not receive their common DNA from a common ancestor, then it disproves the fundamental matching rule upon which all autosomal DNA genetic genealogy is based and we all need to throw in the towel and just go and do something else.

Is there some grey area someplace?  I would presume so,  but at this point, I don’t know how to discern or define it, if there is.  I’ve done three in-depth studies on three different families over the past 6 weeks or so, and I’ve yet to find an area (except for endogamous populations that have matches by population) where the guidelines are problematic.  Other researchers may certainly make different discoveries as they do the same kind of studies.  There is always more to be discovered, so we need to keep an open mind.

In this situation, it helps a lot that the Hickerson/Vannoy descendants match and triangulate on larger segments on other chromosomes.  This study was specifically to see if smaller segments would triangulate and obey the rules. We were fortunate to have such a large, apparently “sticky” segment of Vannoy DNA on chromosome 15 to work with.

Does small segment matching matter in most cases, especially when you have larger segments to utilize?  Probably not. Use the largest segments first.  But in some cases, like where you are trying to prove an ancestor who was born in the 1700s, you may desperately need that small segment data in order to triangulate between three people.

Why is this important – critically important?  Because if small segments obey all of the triangulation rules when larger segments are available to “prove” the match, then there is no reason that they couldn’t be utilized, using the same rules of IBD/IBS, when larger segments are not available.  We saw this in Just One Cousin as well.

However, in terms of proof of concept, I don’t know what better proof could possibly be offered, within the standard genetic genealogy proofs where IBD/IBS guidelines are utilized as described in the Phasing article.  Additional examples of small segment proof by triangulation are offered in Just One Cousin, Lazarus – Putting Humpty Dumpty Together Again, and in Demystifying Autosomal DNA Matching.

Raising Elijah Vannoy and Sarah Hickerson from the Dead

As I thought more about this situation, I realized that I was doing an awful lot of spreadsheet heavy lifting when a tool might already be available.  In fact, Israel’s mention of Lazarus made me wonder if there was a way to apply this tool to the situation at hand.

I decided to take a look at the Lazarus tool and here is what the intro said:

Generate ‘pseudo-DNA kits’ based on segments in common with your matches. These ‘pseudo-DNA kits’ can then be used as a surrogate for a common ancestor in other tests on this site. Segments are included for every combination where a match occurs between a kit in group1 and group2.

It’s obvious from further instructions that this is really meant for a parent or grandparent, but the technique should work just the same for more distant relatives.

I decided to try it first just with the descendants of Elijah Vannoy.  At first, I thought that recreated Elijah would include the following DNA:

  • DNA segments from Elijah Vannoy
  • DNA segments from Elijah Vannoy’s wife, Lois McNiel
  • DNA segments that match from Elijah’s descendants spouse’s lines when individuals come from the same descendant line. This means that if three people descend from Joel Vannoy and Phoebe Crumley, Elijah’s son and his wife, that they would match on some DNA from Phoebe, and that there was no way to subtract Phoebe’s DNA.

After working with the Lazarus tool, I realized this is not the case because Lazarus is designed to utilize a group of direct descendants and then compare the DNA of that group to a second group of know relatives, but not descendants.

In other words, if you have a grandson of a man, and his brother.  The DNA shared by the brother and the grandson HAS to be the DNA contributed to that grandson by his grandfather, from their common ancestor, the great grandfather.  So, in our situation above, Phoebe’s DNA is excluded.

The chart below shows the inheritance path for Lazarus matching.

Lazarus inheritance

Because Lazarus is comparing the DNA of Son Doe with Brother Doe – that eliminates any DNA from the brother’s wives, Sarah Spoon or Mary – because those lines are not shared between Brother Doe and Son Doe.  The only shared ancestors that can contribute DNA to both are Father Doe and Methusaleh Fisher.

The Lazarus instructions allow you to enter the direct descendants of the person/couple that you are reconstructing, then a second set of instructions asks for remaining relatives not directly descended, like siblings, parents, cousins, etc. In other words, those that should share DNA through the common ancestor of the person you are recreating.

To recreate Elijah, I entered all of the Vannoy cousins and then entered William (V) as a sibling since he is the proven son of Daniel Vannoy and Sarah Hickerson.

Here is what Lazarus produced.

lazarus elijah 1

Lazarus includes segments of 4cM and 500 SNPs.

The first thing I thought was, “Holy Moly, what happened to chromosome 15?”  I went back and looked, and sure enough, while almost all of the Elijah descendants do match on chromosome 15, William (V), kit 156020, does not match above the Lazarus threshold I selected.  So chromosome 15 is not included.  Finding additional people who are known to be from this Vannoy line and adding them to the “nondescendant” group would probably result in a more complete Elijah.

lazarus elijah 2

Next, to recreate Sarah Hickerson, I added all of the Vannoy cousins plus William (V) as descendants of Sarah Hickerson and then I added just the one Hickerson descendant, William, as a sibling.  William’s ancestor is proven to be the sibling of Sarah.

I didn’t know quite what to expect.

Clearly if the DNA from the Hickerson descendant didn’t match or triangulate with DNA from any of the Vannoy cousins at this higher level, then Sarah Hickerson wasn’t likely Elijah’s mother.  I wanted to see matching, but more, I wanted to see triangulation.

lazarus elijah 3

I was stunned.  Every kit except two had matches, some of significant size.

lazarus elijah 4

lazarus elijah 5 v2

Please note that locations on chromosomes 3, 4 and 13, above, are triangulated in addition to matching between two individuals, which constitutes proof of a common ancestor.  Please also note that if you were throwing away segments below 7cM, you would lose all of the triangulated matches and all but two matches altogether.

Clearly, comparing the Vannoy DNA with the Hickerson DNA produced a significant number of matches including three triangulated segments.

lazarus elijah 6

Where Are We?

I never have, and I never would recommend attempting to utilize random small match segments out of context.  By out of context, I mean simply looking at all of your 1cM segments and suggesting that they are all relevant to your genealogy.  Nope, never have.  Never would.

There is no question that many small segments are IBS by chance or identical by population.  Furthermore, working with small segments in endogamous populations may not be fruitful.

Those are the caveats.  Small segments in the right circumstances are useful.  And we’ve seen several examples of the right circumstances.

Over the past few weeks, we have identified guidelines and tools to work with small segments, and they are the same tools and guidelines we utilize to work with larger segments as well.  The difference is size.  When working with large segments, the fact that they are large serves an a filter for us and we don’t question their authenticity.  With all small segments, we must do the matching and analysis work to prove validity.  Probably not worthwhile if you have larger segments for the same group of people.

Working with the Vannoy data on chromosome 15 is not random, nor is the family from an endogamous population.  That segment was proven to be Vannoy prior to attempts to confirm or disprove the Hickerson connection.  And we’ve gone beyond just matching, we’ve proven the ancestral link by triangulation, including small segments.  We’ve now proven the Hickerson connection about 7 ways to Sunday.  Ok, maybe 7 is an exaggeration, but here is the evidence summed up for the Vannoy/Hickerson study from multiple vendors and tools:

  • Ancestry DNA Circle indicating that multiple Hickerson descendants match me and some that don’t match me, match each other. Not proof, but certainly suggestive of a common ancestor.
  • A total of 26 Hickerson or derivative family name matches to Vannoy cousins at Family Tree DNA. Not proof, but again, very suggestive.
  • 6 Charles Hickerson/Mary Lytle descendants match to Vannoy cousins at Family Tree DNA. Extremely suggestive, needs triangulation.
  • Triangulation of segments between Vannoy and Hickerson cousins at Family Tree DNA. Proof, but in this study we were only looking to determine whether small segment matches constituted proof.
  • Triangulation of multiple Hickerson/Vannoy cousins on chromosome 15 at GedMatch utilizing small segments and one to one matching. More proof.
  • Lazarus, at higher thresholds than the triangulation matching, when creating Sarah Hickerson, still matched 19 segments and triangulated three for a total of 73.2cM when comparing the Hickerson descendant against the Vannoy cousins. Further proof.

So, can small segment matching data be useful? Is there any reason NOT to accept this evidence as valid?

With proper usage, small segment data certainly looks to provide value by judiciously applying exactly the same rules that apply to all DNA matching.  The difference of course being that you don’t really have to think about utilizing those tools with large segment matches.  It’s pretty well a given that a 20cM match is valid, but you can never assume anything about those small segment matches without supporting evidence. So are larger segments easier to use?  Absolutely.

Does that automatically make small segments invalid?  Absolutely not.

In some cases, especially when attempting to break down brick walls more than 5 or 6 generations in the past, small segment data may be all we have available.  We must use it effectively.  How small is too small?  I don’t know.  It appears that size is really not a factor if you strictly adhere to the IBD/IBS guidelines, but at some point, I would think the segments would be so small that just about everyone would match everyone because we are all humans – so the ultimate identical by population scenario.

Segments that don’t match an individual and either or both parents, assuming you have both parents to test, can safely be disregarded unless they are large and then a look at the raw data is in order to see if there is a problem in that area.  These are IBS by chance.  IBS segments by chance also won’t triangulate further up the tree.  They can’t, because they don’t match your parents so they cannot come from an ancestor.  If they don’t come from an ancestor, they can’t possibly match two other people whose DNA comes from that ancestor on that segment.

If both parents aren’t available, or your small segments do match with your parents, I would suggest that you retain your small segments and map them.

You can’t recognize patterns if the data isn’t present and you won’t be able to find that proverbial needle in the haystack that we are all looking for.

Based on what we’ve seen in multiple case studies, I would conclude that small segment data is certainly valid and can play a valid role in a situation where there is a known or suspected relationship.

I would agree that attempting to utilize small segment data outside the context of a larger data match is not optimal, at least not today, although I wish the vendors would provide a way for us to selectively lower our thresholds.  A larger segment match can point the way to smaller segment matches between multiple people that can be triangulated.  In some situations, like the person A, B, C, D Hickerson-Vannoy situation I described earlier in this article, I would like to be able to drop the match threshold to reveal the small segment data when other matches are suggestive of a family relationship.

In the Hickerson situation, having the ability to drop the matching thresholds would have been the key to positively confirming this relationship within the vendor’s data base and not having to utilize third party tools like GedMatch – which require the cooperation of all parties involved to download their raw data files.  Not everyone transferred their data to Gedmatch in my Vannoy group, but enough did that we were able to do what we needed to do.  That isn’t always the case.  In fact, I have an nearly identical situation in another line but my two matches at Ancestry have declined to download their data to Gedmatch.

This not the first time that small segment data has played a successful role in finding genealogy solutions, or confirming what we thought we knew – although in all cases to date, larger segments matched as well – and those larger segment matches were key and what pointed me to the potential match that ultimately involved the usage of the small segments for triangulation.

Using larger data segments as pointers probably won’t be the case forever, especially if we can gain confidence that we can reliably utilize small segments, at least in certain situations.  Specifically, a small segment match may be nothing, but a small segment triangulated match in the context of a genealogical situation seems to abide by all of the genetic genealogy DNA rules.

In fact, a situation just arose in the past couple weeks that does not include larger segments matching at a vendor.

Let’s close this article by discussing this recent scenario.

The Adoptee

An adoptee approached me with matching data from GedMatch which included matches to me, Dean, Carl and Harold on chromosome 15, on segments that overlap, as follows.

adoptee chr 15

On the spreadsheet above, sent to me by the adoptee, we can see some matches but not all matches. I ran the balance of these 4 people at GedMatch and below is the matching chart for the segment of chromosome 15 where the adoptee matches the 4 Vannoy cousins plus William(H), the Hickerson cousin.

  Me Carl Dean Harold Adoptee
Me NA FTDNA FTDNA GedMatch GedMatch
Carl FTDNA NA FTDNA FTDNA GedMatch
Dean FTDNA FTDNA NA FTDNA GedMatch
Harold GedMatch FTDNA FTDNA NA GedMatch
Adoptee GedMatch GedMatch GedMatch GedMatch NA
William (H) GedMatch GedMatch GedMatch GedMatch GedMatch

I decided to take the easy route and just utilize Lazarus again, so I added all of the known Vannoy and Hickerson cousins I utilized in earlier Lazarus calculations at Gedmatch as siblings to our adoptee.  This means that each kit will be compared to the adoptees DNA and matching segments will be reported.  At a threshold of 300 SNPs and 4cM, our adoptee matches at 140cM of common DNA between the various cousins.

adoptee vannoy match

Please note that in addition to matching several of the cousins, our adoptee also triangulates on chromosomes 1, 11, 15, 18, 19 and 21.  The triangulation on chromosome 21 is to two proven Hickerson descendants, so he matches on this line as well.

I reduced the threshold to 4cM and 200 SNPs to see what kind of difference that would make.

adoptee vannoy match low threshold

Our adoptee picked up another triangulation on chromosome 1 and added additional cousins in the chromosome 15 “sticky Vannoy” cluster and the chromosome 18 cluster.

Given what we just showed about chromosome 15, and the discussions about IBD and IBS guidelines and small matching segments, what conclusions would you draw and what would you do?

  1. Tell the adoptee this is invalid because there are no qualifying large match segments that match at the vendors.
  2. Tell the adoptee to throw all of those small segments away, or at least all of the ones below 7cM because they are only small matching segments and utilizing small matching segments is only a folly and the adoptee is only seeing what he wants to see – even though the Vannoy cousins with whom he triangulates are proven, triangulated cousins.
  3. Check to see if the adoptee also matches the other cousins involved, although he does clearly already exceeds the triangulation criteria to declare a common ancestor of 3 proven cousins on a matching segment. This is actually what I did utilizing Lazarus and you just saw the outcome.

If this is a valid match, based on who he does and doesn’t match in terms of the rest of the family, you could very well narrow his line substantially – perhaps by utilizing the various Vannoy wives’ DNA, to an ancestral couple.  Given that our adoptee matches both the Vannoys and the Hickersons, I suspect he is somehow descended from Daniel Vannoy and Sarah Hickerson.

In Conclusion

What is the acceptable level to utilize small segments in a known or suspected match situation?

Rather than look for a magic threshold number, we are much better served to look at reliable methods to determine the difference between DNA passed from our ancestors to us, IBD, and matches by chance.  This helps us to establish the reliability of DNA segments in individual situations we are likely to encounter in our genealogy.  In other words, rather that throw the entire pile of wheat away because there is some percentage of chaff in the wheat, let’s figure out how to sort the wheat from the chaff.

Fortunately, both parental phasing and triangulation eliminate the identical by chance segments.

Clearly, the smaller the segments, even in a known match situation, the more likely they are identical by population, given that they triangulate.  In fact, this is exactly how the Neanderthal and Denisovan genomes have been reconstructed.

Furthermore, given that the Anzick DNA sample is over 12,000 years old, Identical by population must be how Anzick is matching to contemporary humans, because at least some of these people do clearly share a common ancestor with Anzick at some point, long ago – more than 12,000 years ago.  In my case, at least some of the Anzick segments triangulate with my mother’s DNA, so they are not IBS by chance.  That only leaves identical by population or identical by descent, meaning within a genealogical timeframe, and we know that isn’t possible.

There are yet other situations where small segment matches are not IBS by chance nor identical by population.  For example, I have a very hard time believing that the adoptee situation is nothing but chance.  It’s not a folly.  It’s identical by descent as proven by triangulation with 10 different cousins – all on segments below the vendor matching thresholds.

In fact, it’s impossible to match the Vannoy cousins, who are already triangulated individually, by chance.  While the adoptee match is not over the vendor threshold, the segments are not terribly small and they do all triangulate with multiple individuals who also triangulate with larger segments, at the vendors and on different chromosomes.

This adoptee triangulated match, even without the Hickerson-Vannoy study disproves the blanket statement that small segments below 5cM cannot be used for genealogy.  All of these segments are 7.1cM or below and most are below 5.

This small segment match between my mother and her first cousins also disproves that segments under 5cM can never be used for genealogy.

Two cousins combined

This small segment passed from my mother to me disproves that statement too – clearly matching with our cousin, Cheryl.  If I did not receive this from my mother, and she from her parent, then how do we match a common cousin???

me mother small seg

More small segment proof, below, between my mother and her second cousin when Lazarus was reconstructing my mother’s father.

2nd cousin lazarus match

And this Vannoy Hickerson 4 cousin triangulated segment also disproves that 5cM and below cannot be used for genealogy.

vannoy hickerson triang

Where did these small segments come from if not a common ancestor, either one or several generations ago?  If you look at the small segment I inherited from my mother and say, “well, of course that’s valid, you got it from your mother” then the same logic has to apply that she inherited it from her parent.  The same logic then applies that the same small segment, when shared by my mother’s cousin, also came from the their common grandparents.  One cannot be true without the others being true.  It’s the same DNA. I got it from my mother.  And it’s only a 1.46cM segment, shown in the examples above.

Here are my observations and conclusions:

  • As proven with hundreds of examples in this and other articles cited, small segments can be and are inherited from our ancestors and can be utilized for genetic genealogy.
  • There is no line in the sand at 7cM or 5cM at which a segment is viable and useful at 5.1cM and not at 4.9cM.
  • All small segment matches need to be evaluated utilizing the guidelines set forth for IBD versus IBS by chance versus identical by population set forth in the articles titled How Phasing Works and Determining IBD Versus IBS Matches and Demystifying Autosomal DNA Matching.
  • When given a choice, large segment matches are always easier to use because they are seldom IBS by chance and most often IBD.
  • Small segment matches are more likely to be IBS by chance than larger matches, which is why we need to judiciously apply the IBD/IBS Guidelines when attempting to utilize small segment matches.
  • All DNA matches, not just small segments, must be triangulated to prove a common ancestor, unless they are known close relatives, like siblings, first cousins, etc.
  • When working in genetic genealogy, always glean the information from larger matches and assemble that information.  However, when the time comes that you need those small segments because you are working 5, 6 or 7 generations back in time, remember that tools and guidelines exist to use small segments reliably.
  • Do not attempt to use small segments out of context.  This means that if you were to look only at your 1cM matches to unknown people, and you have the ability to triangulate against your parents, most would prove to be IBS by chance.  This is the basis of the argument for why some people delete their small segments.  However, by utilizing parental phasing, phasing against known family members (like uncles, aunts and first cousins) and triangulation, you can identify and salvage the useable small segments – and these segments may be the only remnants of your ancestors more than 5 or 6 generations back that you’ll ever have to work with.  You do not have to throw all of them away simply because some or many small segments, out of context, are IBS by chance.  It doesn’t hurt anything to leave them just sit in your spreadsheet untouched until the day that you need them.

Ultimately, the decision is yours whether you will use small segments or not – and either decision is fine.  However, don’t make the decision based on the belief that small segments under some magic number, like 5cM or 7cM are universally useless.  They aren’t.

Whether small segments are too much work and effort in your individual situation depends on your personal goals for genetic genealogy and on factors like whether or not you descend from an endogamous population.  People’s individual goals and circumstances vary widely.  Some people test at Ancestry and are happy with inferential matching circles and nothing more.  Some people want to wring every tidbit possible out of genealogy, genetic or otherwise.

I hope everyone will begin to look at how they can use small segment data reliably instead of simply discarding all the small segments on the premise that all small segment data is useless because some small segments are not useful.  All unstudied and discarded data is indeed useless, so discarding becomes a self-fulfilling prophecy.

But by far, the worst outcome of throwing perfectly good data away is that you’ll never know what genetic secrets it held for you about your ancestors.  Maybe the DNA of your own Sarah Hickerson is lurking there, just waiting for the right circumstances to be found.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

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Anzick Matching Update

In response to my article about haplogroup C3*, a regular contributor, Armando, left the following comment:

“Roberta, there was a problem with the way Felix was processing files and he had to change the Clovis Anzick file three times at Gedmatch. The last one is kit F999919 uploaded October 8, 2014. You can see his post on that at http://www.fc.id.au/2014/10/new-clovis-anzick-1-kit-in-gedmatch.html

If you do one-to-many matching on Clovis Anzick F999919 at Gedmatch there is not a single person that reports to have mtDNA M. Your extracts for Clovis Anzick are from September 24, 2014 and therefore are based on a bad file which was kit F999912. The older bad kits F999912 and F999913 have been deleted from Gedmatch. Felix mentions the updates at http://www.fc.id.au/2014/09/clovis-anzick-1-dna-match-living-people.html

This comment came in on Christmas Eve, and I replied that I would look into this after the holidays.

Given that it was Christmas Eve, I certainly wasn’t going to bother anyone over the holidays with questions, so I quickly ran a one to many compare for the current Anzick kit, F999919, and found at 5cM and below that there were 4 haplogroup M matches.

ancient match

As I did before, I sent emails to those who provided e-mail addresses asking about their matrilineal heritage.

The first thing I wanted to do, of course, was to check with Felix.  I knew that Felix had updated the kits, but my understanding was that he added SNPs from the various companies to create a single file with all the SNPs from all three testing companies, not that any file was bad, so to speak.

I asked Felix if the original files had problems or were bad, and here is his response.

“I can assure you none of the earlier/older versions uploaded to GEDmatch (kit# F999912 and F999913) of Clovis Anzick was bad.

  • F999912 – Contains only FTDNA SNPs extracted from VCF file provided by authors.
  • F999913 – Contains all SNPs used by DNA testing companies extracted from VCF file provided by authors.
  • F999919 – Contains all SNPs used by DNA testing companies processed from BAM file provided by authors.

Source files: 

I removed the earlier versions not because they are bad but only to avoid redundancy for the same sample kit, and processed BAM file (which is a 41 GB file) contains significantly more SNPs compared to VCF source. Because the latest file has more SNPs, it is possible that some missing SNPs in earlier uploads (which was assumed as matching in GEDmatch) may actually have mismatches in new file and thus, could fall below the thresholds or could break the previously matching segment.

The difference in matches between F999912 and F999919 kit for Clovis Anzick is similar to difference in matches between a 23andMe V4 kit and V3 kit for the same person.”

After thinking about this some, it occurred to me that perhaps GedMatch was treating different files from different vendors differently in their matching and sorting routines. That might account for a difference in matching. So, I asked John Olson at GedMatch.

John’s reply is as follows:

“At one time, I did use different thresholds depending on which vendor was being compared to which other vendor.  That was a holdover from when FTDNA had Affymetrix kits that were producing somewhat different results than Illumina kits.  I have since changed the one-to-many thresholds to 5cM/500 SNPs for all comparisons.  The one-to-one thresholds default to 7cm/700 SNPs.  I believe I made that change about a year ago, but it may have been longer.  At any rate, they are all the same now, and I’m pretty sure they are all the same since Felix has introduced the F9999xx kits.  Another change made within the past year is to treat A=T and C=G for all comparisons.  This was done to get rid of single SNP errors in the few cases where one vendor was reporting a different strand than another vendor.  In a few cases, I have observed that this “heals” some single-SNP breaks in otherwise continuous matching segments.

It is possible that older one-to-many comparisons may have been made under slightly different conditions than newer ones.  Older comparisons made with a 3cm/300 SNP threshold may show larger total segment match if they contained many very small matching segments.  This usually happens with endogamous populations.  Comparisons affected by the change to A=T, C=G may show a larger matching segment where 2 smaller matching segments existed previously.

Another issue to be aware of when comparing artificial kits is that there may be large gaps between the defined SNPs.  So, even if there is a gap of a million SNPs, the GEDmatch comparison algorithm will treat them as contiguous.  This works OK when everybody is using the same SNPs, but when the list of SNPs is significantly different, it may produce matches that are bogus.  This is particularly obvious when generating artificial kits that are missing large segments of data.  I have had to deal with this issue with phased kits and Lazarus kits by introducing the concept of a “hard break” that forces a break between smaller matching segments.”

I wanted to know how the three files that Felix prepared compared relative to the matches they produced.  I originally ran several comparisons with each of the first two versions, kits F999912 and F999913, and I didn’t save all of the original files, but I do have at least one file saved from each version.  Therefore, I dropped all three sets of results (F999912, F999913 and F999919) into a spreadsheet to see how matching compared between the three Anzick file versions.

Keep in mind that the first file (F999912) contained just the FTDNA SNPs, while the second (F999913) and third (F999919) files contain the SNPs from all of the testing companies.  This could potentially make the participant files appear to have missing segments when the matching routine at GedMatch sees SNPs in the Anzick file not in the participant files.  However, this shouldn’t be much different than comparing a file from two different vendors except that the Anzick file has the SNPs from all three vendors combined.

The first file from 9-23 at the default threshold had 491 matches, but I subsequently lowered the threshold so I could see as many matches as possible.

GedMatch only shows you your closest 1500 matches, although I now know that as of 12-31-2014, there were a total of 3442 Anzick matches at the 5cM threshold.

The second file from 9-29, run at 6cM had more than 1500 matches.  I ran the third kit at default settings on December 27th and it has 720 matches.

One would expect that the second and third files would have the effect of including more matches from both 23andMe and Ancestry since all of the SNPs utilized by those companies are included (if they are available in the Anzick sample.)  We also have to remember that there are new files being uploaded from all three vendor sites on a daily basis, so the total available to match is also increasing.  Of the 721 kit matches to F999919, 31 were shades of green which indicate that they have been uploaded during the last 30 days, so we could probably presume that about double that number were uploaded (and match) in two months or triple in three months, so probably about 100 new kits.  Those kits would show in the match extraction for this month but not for the first month and possibly not for the second.  However, all the kits that matched the first month at the highest threshold should still be showing in the second and third month.  Let’s see if that holds true.

I dropped all three sets of data into a spreadsheet and colorized the rows.

ancient match 1

  • Blue = F999912, first extraction, 9-23-2014
  • Yellow = F999913, second extraction, 9-29-2014
  • Pink = F999919, third extraction, 12-27-2014

Then I counted the number of blue rows, which are the first extraction, that had matches to both yellow and pink, or only yellow, the second extraction, or only pink, the third (current) extraction, or no matches at all.

You can see that the green grouping shows that all three match each other.  The match between A003479 in both the second and third extraction could be because the kit was not present when the first extraction was done.

All 3 match 1st to 2nd Only 1st  to 3rd Only No Match
Percent First Extraction Matches to Other Extractions 54% 36% 5% 5%

By percent, this is how the matching between kits worked.  About half of the kits in the first extraction continued to match kits in both subsequent extractions.  Of the remaining half, three quarters of the balance matches the second extraction only and a few match just the third extraction or no extraction at all.  For the most part, there is no evident reason upon inspection why the kits would not match the second or third extraction, so the cause has to be a result of the additional SNPs or the matching routine or both.  This is not to imply that the results are problematic, just that they are different than I would have expected.

A very low percentage of kits matched only between the first and third extracts and the same percentage had no matches in either the second or third extraction.

I took a closer look at the kits with no matches at all.  All of them had relatively low threshold total cM and largest segment size.  The smallest total cM was 7.1 and the largest was 8.2.  The smallest segment was 7.1 and the largest segment was also 8.2.  All of these entries had the total cM equal to the largest cM.  It appears that these simply slipped below the match threshold, but that doesn’t appear to be the case because in the current (pink) extract, a total of 171 entries were at or below 8.2 total cM and 8.2 largest cM and several kits had the exact same cM as the kits that didn’t show up from the first (blue) extract as a match – so obviously something truly was different in the SNPs or how the matching was done.

Is there any correlation to the kits in the original extract that didn’t match any other extract in terms of which testing company the participants utilized?

One Ancestry kit (4%), 18 23andMe kits (64%), 7 Family Tree DNA kits (25%) and 2 FN kits (7%) didn’t match anyone.  But how many kits were in the original extract from the various companies?

Original Kit Matches Second KitMatches Current Kit Matches
Ancestry Kits (A) 26 (5%) 438 (29%) 199 (28%)
FTDNA Kits (F) 94 (19%) 295 (20%) 121 (17%)
Other F+ Kits* 15 (3%) 35 (2%) 15 (2%)
23andMe Kits (M) 354 (72%) 732 (49%) 382 (53%)

*FB, FN, FE, FV

The effect of the additional SNPs in the kits seems to have been to increase the Ancestry kit matches significantly.

It was interesting to see how the same person’s kit from different vendors compared as well.  In this random example, the Family Finder kit has a higher total cM and largest segment than the 23andMe v3 kit.

ancient match 2

Here’s a kit from one person at all three vendors, but the 23andMe kit is version 4, in which 23andMe significantly reduced the number of SNPs tested by about one third, from about 900,000 to about 600,000.

ancient match 3

I wondered if there is a difference in what is reported based on the threshold selected.  Now at first glance, one would think, “well of course there is a difference,” but the difference should be on the bottom end of the list.  In other words, the top matches should be the top matches at 7cM, 6cM, 5cM, etc.  The top matches at 7cM would still be the top at 6cM, just more smaller matches appended to the end of the match list – or that is what I would expect.

Let’s see if this holds true with the current file.

I ran the “one to many” option for the current Anzick kit, F999919, at seven different levels, on the same day, one right after the other, as follows:

  • 7cM, 700 SNPs
  • 6cM, 600 SNPs
  • 5cM, 500 SNPs
  • 4cM, 400 SNPs
  • 3cM, 300 SNPs
  • 2cM, 200 SNPs
  • 1cM, 100 SNPs

The first extract produced 719 records.  The rest were all over the 1500 threshold, so we only see the first 1500.  Normally, for genealogy the 1500 threshold would certainly be adequate, but for research, the threshold is frustrating.

To make this easier let me say that the extracts from 5cM down through 1cM were exactly the same, but the extracts at 7, 6 and 5cM, respectively, were not.

Discussions with John Olson at GedMatch shed some light on why the 5cM through 1cM extracts were exactly the same.

 “For the past year or so, the database has only stored matches down to 5 cM.”

I sure wish I had known that BEFORE I did all of those extracts.

I combined and color coded all 7 extractions into a spreadsheet.

Most of the grouping look like this where blue=7cm, pink=6cM, grn=5cM, purple=4cm, teal=3cm,apricot=2cm, yellow=1cm.  Nice rainbows.

ancient match 4

All of the matches from the 7cM extraction, with the exception of a few X matches at the end, some of which have no matches on chromosomes 1-22, are included in the 6cM and 5cM extractions, but after the first several records, they are not in the same position.  In other words, they are not the top 719, in the same order, in either the 5 or 6cM extraction, but the 5cM through 1cM extractions are identical.  Of course, now we know why the 5cM through 1cM matches are exact. From here forth in the article, I won’t mention the 4cM-1cM extracts because they are the same as the 5cM extract.

For example, looking at the kit in position 712, the last non-X match in the 7cM extract – you find this same kit at row 1140 in the 6cM extract and row 1489 in the 5cM extract.

The 6cM extract appears to have some issues.  I ran this twice with the same parameters to be sure there wasn’t an error in how it was set up, and the two runs were identical.

There are about 350 individuals who show up in the 6cM extract who should  show up in the 5cM extract as well, but who don’t show in the 5cM extract.  They are under the threshold for the 7cM extract, so that is correct, but why are these 350 individuals not appearing as matches at the 5cM threshold?

ancient match 5

The kits noted above are the largest non-matching total cM and largest cM that don’t show up in the 5cM extract.  The smallest matches are 6.1 and 6.1, respectively.

Checking the 5cM extract, below, there are files with smaller total cMs and a smaller largest segment that are showing as matches.

ancient match 6

However, looking at the kits with the smallest cMs at the 5cM level, the smallest total cMs is 6.9 and it is combined with the largest segment of 6.9 as well, so that is above the 6.8 and 6.8 shown above.  The smallest individual segment is 5.1 but the total cM for that individual is 10.1.  So obviously the matching threshold at GedMatch is some combination of both the total cM and the largest segment.  This is somewhat unexpected, but doesn’t seem to be a red flag, just how this system works.

So, where are we?

I am glad to have Felix confirm that the files weren’t “bad,” only truly “new and improved,” and that the matching between the various files is pretty much as expected – and from various tests run, everything pretty much looks kosher.  The newer files with all of the SNPs utilized by the companies seem to level the playing field, allowing Ancestry kits a better chance of matching.

Aside from my intense interest due to the Native American connection, this is also how I’ve been extracting potential Native American mitochondrial haplogroups from the Anzick matches, including haplogroup M, for my research notes.  M is potentially a Native American haplogroup, but is as yet unproven.  With haplogroup M showing up in these people who are often heavily Native, and often from Mexico, Central and South America where 80% of the mitochondrial population is believed to be of Native American heritage, it seems prudent to add them to my research notes for further research and possible proof in the future.  I contact individuals and ask about their matrilineal heritage.  If they don’t have Asian or genealogically proven heritage elsewhere, and their families emerge from the areas with high Native frequencies, I include them on the research list.

In the three days between the two extracts this past week, three of the four haplogroup M individuals were pushed below the match threshold and are no longer visible at the default level.  Yes, I have confirmed hat they are still there just not visible at the 1500 match threshold.

I have contacted the individuals with e-mail addresses, asking about their matrilineal heritage.  One person said the tester’s mother’s heritage was from India, so that haplogroup M is not on the research list, of course, because it is proven to be from elsewhere – a place where haplogroup M and subgroups are quite common.

In total, there were 15 new potentially Native DNA mitochondrial DNA haplogroups listed in the 12-27 extract.  I’ll be adding those to my research notes as soon as I have the opportunity to contact these folks and ask about their known matrilineal genealogy.

I didn’t really anticipate that there would be so much change, nor so quickly, so it looks like I’m going to have to check the Anzick matches for potential Native mitochondrial haplogroups much more often.

Since it looks like there may be lots of additions over time, far more than I expected, I’ll also be going back and making better notes in my research file.  I will, for example, note the kit number and date for all of the extractions.  For this and future extractions, I’ll also be listing the number of results per haplogroup.  I think that would be valuable information as well.

I’d like to thank Armando for raising this topic.  The research into matching with a kit that has the entire spectrum of SNPs from all three of the companies has been quite interesting.  In fact, unless Felix has added all of the SNPs to the other ancient kits, this is the only kit in existence that has all of the SNPs from all of the companies included.

My thanks to Felix Immanuel (formerly Felix Chandrakumar) and John Olson for assistance with research for this article.

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Disclosure

I receive a small contribution when you click on some of the links to vendors in my articles. This does NOT increase the price you pay but helps me to keep the lights on and this informational blog free for everyone. Please click on the links in the articles or to the vendors below if you are purchasing products or DNA testing.

Thank you so much.

DNA Purchases and Free Transfers

Genealogy Services

Genealogy Research